Journal of cell science最新文献_第3页

DRAK2 regulates myosin light chain phosphorylation in T cells. DRAK2 可调节 T 细胞中肌球蛋白轻链的磷酸化。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-10-18 DOI: 10.1242/jcs.261813

Benjamin A Wilander, Tarsha L Harris, Alexandra H Mandarano, Cliff S Guy, Mollie S Prater, Shondra M Pruett-Miller, Stacey K Ogden, Maureen A McGargill

{"title":"DRAK2 regulates myosin light chain phosphorylation in T cells.","authors":"Benjamin A Wilander, Tarsha L Harris, Alexandra H Mandarano, Cliff S Guy, Mollie S Prater, Shondra M Pruett-Miller, Stacey K Ogden, Maureen A McGargill","doi":"10.1242/jcs.261813","DOIUrl":"https://doi.org/10.1242/jcs.261813","url":null,"abstract":"Death-associated protein kinase-related apoptosis-inducing kinase-2 (DRAK2 or STK17B) is a serine/threonine kinase expressed in T cells. Drak2-deficient (Drak2-/-) mice respond effectively to tumors and pathogens while displaying resistance to T cell-mediated autoimmune disease. However, the molecular mechanisms by which DRAK2 impacts T cell function remain unclear. Gaining further insight into the function of DRAK2 in T cells will shed light on differentially regulated pathways in autoreactive and pathogen-specific T cells, which is critical for improving autoimmune therapies. Here, we demonstrate that DRAK2 contributes to activation of myosin light chain (MLC) in both murine and human T cells. In the absence of Drak2, the amount of polymerized actin was decreased, suggesting that DRAK2 modulates actomyosin dynamics. We further show that myosin-dependent T cell functions, such as migration, T cell receptor microcluster accumulation, and conjugation to antigen presenting cells are decreased in the absence of Drak2. These findings reveal that DRAK2 plays an important role in regulating MLC activation within T cells.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

cellPLATO - an unsupervised method for identifying cell behaviour in heterogeneous cell trajectory data. cellPLATO：在异质细胞轨迹数据中识别细胞行为的无监督方法。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-10-15 Epub Date: 2024-06-12 DOI: 10.1242/jcs.261887

Michael J Shannon, Shira E Eisman, Alan R Lowe, Tyler F W Sloan, Emily M Mace

{"title":"cellPLATO - an unsupervised method for identifying cell behaviour in heterogeneous cell trajectory data.","authors":"Michael J Shannon, Shira E Eisman, Alan R Lowe, Tyler F W Sloan, Emily M Mace","doi":"10.1242/jcs.261887","DOIUrl":"10.1242/jcs.261887","url":null,"abstract":"Advances in imaging, segmentation and tracking have led to the routine generation of large and complex microscopy datasets. New tools are required to process this 'phenomics' type data. Here, we present 'Cell PLasticity Analysis Tool' (cellPLATO), a Python-based analysis software designed for measurement and classification of cell behaviours based on clustering features of cell morphology and motility. Used after segmentation and tracking, the tool extracts features from each cell per timepoint, using them to segregate cells into dimensionally reduced behavioural subtypes. Resultant cell tracks describe a 'behavioural ID' at each timepoint, and similarity analysis allows the grouping of behavioural sequences into discrete trajectories with assigned IDs. Here, we use cellPLATO to investigate the role of IL-15 in modulating human natural killer (NK) cell migration on ICAM-1 or VCAM-1. We find eight behavioural subsets of NK cells based on their shape and migration dynamics between single timepoints, and four trajectories based on sequences of these behaviours over time. Therefore, by using cellPLATO, we show that IL-15 increases plasticity between cell migration behaviours and that different integrin ligands induce different forms of NK cell migration.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11213520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140911834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Making the most of bioimaging data through interdisciplinary interactions. 通过跨学科互动充分利用生物成像数据。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-10-15 Epub Date: 2024-10-23 DOI: 10.1242/jcs.262139

Virginie Uhlmann, Matthew Hartley, Josh Moore, Erin Weisbart, Assaf Zaritsky

引用次数: 0

Gut Analysis Toolbox - automating quantitative analysis of enteric neurons. 肠道分析工具箱：肠道神经元定量分析自动化

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-10-15 Epub Date: 2024-10-30 DOI: 10.1242/jcs.261950

Luke Sorensen, Adam Humenick, Sabrina S B Poon, Myat Noe Han, Narges S Mahdavian, Matthew C Rowe, Ryan Hamnett, Estibaliz Gómez-de-Mariscal, Peter H Neckel, Ayame Saito, Keith Mutunduwe, Christie Glennan, Robert Haase, Rachel M McQuade, Jaime P P Foong, Simon J H Brookes, Julia A Kaltschmidt, Arrate Muñoz-Barrutia, Sebastian K King, Nicholas A Veldhuis, Simona E Carbone, Daniel P Poole, Pradeep Rajasekhar

{"title":"Gut Analysis Toolbox - automating quantitative analysis of enteric neurons.","authors":"Luke Sorensen, Adam Humenick, Sabrina S B Poon, Myat Noe Han, Narges S Mahdavian, Matthew C Rowe, Ryan Hamnett, Estibaliz Gómez-de-Mariscal, Peter H Neckel, Ayame Saito, Keith Mutunduwe, Christie Glennan, Robert Haase, Rachel M McQuade, Jaime P P Foong, Simon J H Brookes, Julia A Kaltschmidt, Arrate Muñoz-Barrutia, Sebastian K King, Nicholas A Veldhuis, Simona E Carbone, Daniel P Poole, Pradeep Rajasekhar","doi":"10.1242/jcs.261950","DOIUrl":"10.1242/jcs.261950","url":null,"abstract":"The enteric nervous system (ENS) consists of an extensive network of neurons and glial cells embedded within the wall of the gastrointestinal (GI) tract. Alterations in neuronal distribution and function are strongly associated with GI dysfunction. Current methods for assessing neuronal distribution suffer from undersampling, partly due to challenges associated with imaging and analyzing large tissue areas, and operator bias due to manual analysis. We present the Gut Analysis Toolbox (GAT), an image analysis tool designed for characterization of enteric neurons and their neurochemical coding using two-dimensional images of GI wholemount preparations. GAT is developed in Fiji, has a user-friendly interface, and offers rapid and accurate segmentation via custom deep learning (DL)-based cell segmentation models developed using StarDist, as well as a ganglia segmentation model in deepImageJ. We apply proximal neighbor-based spatial analysis to reveal differences in cellular distribution across gut regions using a public dataset. In summary, GAT provides an easy-to-use toolbox to streamline routine image analysis tasks in ENS research. GAT enhances throughput, allowing rapid unbiased analysis of larger tissue areas, multiple neuronal markers and numerous samples.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of a novel uropod-like cell membrane protrusion in the pathogenesis of the parasite Trichomonas vaginalis. 新型尿囊样细胞膜突起在阴道毛滴虫致病过程中的作用。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-10-15 Epub Date: 2024-09-09 DOI: 10.1242/jcs.262210

Manuela Blasco Pedreros, Nehuen Salas, Tuanne Dos Santos Melo, Abigail Miranda-Magalhães, Thainá Almeida-Lima, Antonio Pereira-Neves, Natalia de Miguel

{"title":"Role of a novel uropod-like cell membrane protrusion in the pathogenesis of the parasite Trichomonas vaginalis.","authors":"Manuela Blasco Pedreros, Nehuen Salas, Tuanne Dos Santos Melo, Abigail Miranda-Magalhães, Thainá Almeida-Lima, Antonio Pereira-Neves, Natalia de Miguel","doi":"10.1242/jcs.262210","DOIUrl":"10.1242/jcs.262210","url":null,"abstract":"Trichomonas vaginalis causes trichomoniasis, the most common non-viral sexually transmitted disease worldwide. As an extracellular parasite, adhesion to host cells is essential for the development of infection. During attachment, the parasite changes its tear ovoid shape to a flat ameboid form, expanding the contact surface and migrating through tissues. Here, we have identified a novel structure formed at the posterior pole of adherent parasite strains, resembling the previously described uropod, which appears to play a pivotal role as an anchor during the attachment process. Moreover, our research demonstrates that the overexpression of the tetraspanin T. vaginalis TSP5 protein (TvTSP5), which is localized on the cell surface of the parasite, notably enhances the formation of this posterior anchor structure in adherent strains. Finally, we demonstrate that parasites that overexpress TvTSP5 possess an increased ability to adhere to host cells, enhanced aggregation and reduced migration on agar plates. Overall, these findings unveil novel proteins and structures involved in the intricate mechanisms of T. vaginalis interactions with host cells.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multiscale chromatin dynamics and high entropy in plant iPSC ancestors. 植物 iPSC 祖先的多尺度染色质动力学和高熵。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-10-15 Epub Date: 2024-06-24 DOI: 10.1242/jcs.261703

Kinga Rutowicz, Joel Lüthi, Reinoud de Groot, René Holtackers, Yauhen Yakimovich, Diana M Pazmiño, Olivier Gandrillon, Lucas Pelkmans, Célia Baroux

{"title":"Multiscale chromatin dynamics and high entropy in plant iPSC ancestors.","authors":"Kinga Rutowicz, Joel Lüthi, Reinoud de Groot, René Holtackers, Yauhen Yakimovich, Diana M Pazmiño, Olivier Gandrillon, Lucas Pelkmans, Célia Baroux","doi":"10.1242/jcs.261703","DOIUrl":"10.1242/jcs.261703","url":null,"abstract":"Plant protoplasts provide starting material for of inducing pluripotent cell masses that are competent for tissue regeneration in vitro, analogous to animal induced pluripotent stem cells (iPSCs). Dedifferentiation is associated with large-scale chromatin reorganisation and massive transcriptome reprogramming, characterised by stochastic gene expression. How this cellular variability reflects on chromatin organisation in individual cells and what factors influence chromatin transitions during culturing are largely unknown. Here, we used high-throughput imaging and a custom supervised image analysis protocol extracting over 100 chromatin features of cultured protoplasts. The analysis revealed rapid, multiscale dynamics of chromatin patterns with a trajectory that strongly depended on nutrient availability. Decreased abundance in H1 (linker histones) is hallmark of chromatin transitions. We measured a high heterogeneity of chromatin patterns indicating intrinsic entropy as a hallmark of the initial cultures. We further measured an entropy decline over time, and an antagonistic influence by external and intrinsic factors, such as phytohormones and epigenetic modifiers, respectively. Collectively, our study benchmarks an approach to understand the variability and evolution of chromatin patterns underlying plant cell reprogramming in vitro.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11234377/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140911837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expansion microscopy reveals characteristic ultrastructural features of pathogenic budding yeast species. 膨胀显微镜揭示了致病芽殖酵母物种特有的超微结构特征。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-10-15 Epub Date: 2024-09-09 DOI: 10.1242/jcs.262046

Md Hashim Reza, Srijana Dutta, Rohit Goyal, Hiral Shah, Gautam Dey, Kaustuv Sanyal

{"title":"Expansion microscopy reveals characteristic ultrastructural features of pathogenic budding yeast species.","authors":"Md Hashim Reza, Srijana Dutta, Rohit Goyal, Hiral Shah, Gautam Dey, Kaustuv Sanyal","doi":"10.1242/jcs.262046","DOIUrl":"10.1242/jcs.262046","url":null,"abstract":"Candida albicans is the most prevalent fungal pathogen associated with candidemia. Similar to other fungi, the complex life cycle of C. albicans has been challenging to study with high-resolution microscopy due to its small size. Here, we employed ultrastructure expansion microscopy (U-ExM) to directly visualise subcellular structures at high resolution in the yeast and during its transition to hyphal growth. N-hydroxysuccinimide (NHS)-ester pan-labelling in combination with immunofluorescence via snapshots of various mitotic stages provided a comprehensive map of nucleolar and mitochondrial segregation dynamics and enabled the resolution of the inner and outer plaque of spindle pole bodies (SPBs). Analyses of microtubules (MTs) and SPBs suggest that C. albicans displays a side-by-side SPB arrangement with a short mitotic spindle and longer astral MTs (aMTs) at the pre-anaphase stage. Modifications to the established U-ExM protocol enabled the expansion of six other human fungal pathogens, revealing that the side-by-side SPB configuration is a plausibly conserved feature shared by many fungal species. We highlight the power of U-ExM to investigate subcellular organisation at high resolution and low cost in poorly studied and medically relevant microbial pathogens.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11423813/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141758958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Illuminating cellular architecture and dynamics with fluorescence polarization microscopy. 用荧光偏振显微镜观察细胞结构和动态。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-10-15 Epub Date: 2024-10-14 DOI: 10.1242/jcs.261947

William F Dean, Alexa L Mattheyses

{"title":"Illuminating cellular architecture and dynamics with fluorescence polarization microscopy.","authors":"William F Dean, Alexa L Mattheyses","doi":"10.1242/jcs.261947","DOIUrl":"10.1242/jcs.261947","url":null,"abstract":"Ever since Robert Hooke's 17th century discovery of the cell using a humble compound microscope, light-matter interactions have continuously redefined our understanding of cell biology. Fluorescence microscopy has been particularly transformative and remains an indispensable tool for many cell biologists. The subcellular localization of biomolecules is now routinely visualized simply by manipulating the wavelength of light. Fluorescence polarization microscopy (FPM) extends these capabilities by exploiting another optical property - polarization - allowing researchers to measure not only the location of molecules, but also their organization or alignment within larger cellular structures. With only minor modifications to an existing fluorescence microscope, FPM can reveal the nanoscale architecture, orientational dynamics, conformational changes and interactions of fluorescently labeled molecules in their native cellular environments. Importantly, FPM excels at imaging systems that are challenging to study through traditional structural approaches, such as membranes, membrane proteins, cytoskeletal networks and large macromolecular complexes. In this Review, we discuss key discoveries enabled by FPM, compare and contrast the most common optical setups for FPM, and provide a theoretical and practical framework for researchers to apply this technique to their own research questions.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":null,"pages":null},"PeriodicalIF":3.3,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11529880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Imaging interorganelle contacts at a glance. 器官间接触成像一目了然。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-10-15 Epub Date: 2024-10-23 DOI: 10.1242/jcs.262020

Maria Clara Zanellati, Chih-Hsuan Hsu, Sarah Cohen

引用次数: 0

I am not a microscopist. 我不是显微镜专家。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-10-15 Epub Date: 2024-10-23 DOI: 10.1242/jcs.263587

Seema Grewal, Helen L Zenner

引用次数: 0