Journal of cell science最新文献_第3页

An image-based RNAi screen identifies the EGFR signaling pathway as a regulator of Imp RNP granules. 基于图像的 RNAi 筛选确定 EGF.R 信号通路是 Imp/ IGF2BP RNP 颗粒的调节因子。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-10 DOI: 10.1242/jcs.262119

Fabienne De Graeve, Eric Debreuve, Kavya Vinayan Pushpalatha, Xuchun Zhang, Somia Rahmoun, Djampa Kozlowski, Nicolas Cedilnik, Jeshlee Vijayakumar, Paul Cassini, Sebastien Schaub, Xavier Descombes, Florence Besse

{"title":"An image-based RNAi screen identifies the EGFR signaling pathway as a regulator of Imp RNP granules.","authors":"Fabienne De Graeve, Eric Debreuve, Kavya Vinayan Pushpalatha, Xuchun Zhang, Somia Rahmoun, Djampa Kozlowski, Nicolas Cedilnik, Jeshlee Vijayakumar, Paul Cassini, Sebastien Schaub, Xavier Descombes, Florence Besse","doi":"10.1242/jcs.262119","DOIUrl":"10.1242/jcs.262119","url":null,"abstract":"Biomolecular condensates have recently retained much attention given that they provide a fundamental mechanism of cellular organization. Among those, cytoplasmic ribonucleoprotein (RNP) granules selectively and reversibly concentrate RNA molecules and regulatory proteins, thus contributing to the spatiotemporal regulation of associated RNAs. Extensive in vitro work has unraveled the molecular and chemical bases of RNP granule assembly. The signaling pathways controlling this process in a cellular context are, however, still largely unknown. Here, we aimed at identifying regulators of cytoplasmic RNP granules characterized by the presence of the evolutionarily conserved Imp RNA-binding protein (a homolog of IGF2BP proteins). We performed a high-content image-based RNAi screen targeting all Drosophila genes encoding RNA-binding proteins, phosphatases and kinases. This led to the identification of dozens of genes regulating the number of Imp-positive RNP granules in S2R+ cells, among which were components of the MAPK pathway. Combining functional approaches, phospho-mapping and generation of phospho-variants, we further showed that EGFR signaling inhibits Imp-positive RNP granule assembly through activation of the MAPK-ERK pathway and downstream phosphorylation of Imp at the S15 residue. This work illustrates how signaling pathways can regulate cellular condensate assembly by post-translational modifications of specific components.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The dual Ras-association domains of Drosophila Canoe have differential roles in linking cell junctions to the cytoskeleton during morphogenesis. 果蝇 Canoe 的双 Ras 关联（RA）域在形态发生过程中连接细胞连接和细胞骨架方面具有不同的作用。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-11 DOI: 10.1242/jcs.263546

Emily D McParland, Noah J Gurley, Leah R Wolfsberg, T Amber Butcher, Abhi Bhattarai, Corbin C Jensen, Ruth I Johnson, Kevin C Slep, Mark Peifer

{"title":"The dual Ras-association domains of Drosophila Canoe have differential roles in linking cell junctions to the cytoskeleton during morphogenesis.","authors":"Emily D McParland, Noah J Gurley, Leah R Wolfsberg, T Amber Butcher, Abhi Bhattarai, Corbin C Jensen, Ruth I Johnson, Kevin C Slep, Mark Peifer","doi":"10.1242/jcs.263546","DOIUrl":"10.1242/jcs.263546","url":null,"abstract":"During development cells must change shape and move without disrupting dynamic tissue architecture. This requires robust linkage of cell-cell adherens junctions to the force-generating actomyosin cytoskeleton. Drosophila Canoe and mammalian afadin play key roles in the regulation of such linkages. One central task for the field is defining mechanisms by which upstream inputs from Ras-family GTPases regulate Canoe and afadin. These proteins are unusual in sharing two tandem Ras-association (RA) domains - RA1 and RA2 - which when deleted virtually eliminate Canoe function. Work in vitro has suggested that RA1 and RA2 differ in GTPase affinity, but their individual functions in vivo remain unknown. Combining bioinformatic and biochemical approaches, we find that both RA1 and RA2 bind to active Rap1 with similar affinities, and that their conserved N-terminal extensions enhance binding. We created Drosophila canoe mutants to test RA1 and RA2 function in vivo. Despite their similar affinities for Rap1, RA1 and RA2 play strikingly different roles. Deleting RA1 virtually eliminates Canoe function, whereas mutants lacking RA2 are viable and fertile but have defects in junctional reinforcement in embryos and during pupal eye development. These data significantly expand our understanding of the regulation of adherens junction-cytoskeletal linkages.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Topology surveillance of the lanosterol demethylase CYP51A1 by signal peptide peptidase. 信号肽肽酶对羊毛甾醇脱甲基酶 CYP51A1 的拓扑监测

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-12 DOI: 10.1242/jcs.262333

Nikita Sergejevs, Dönem Avci, Michael L van de Weijer, Robin A Corey, Marius K Lemberg, Pedro Carvalho

引用次数: 0

Cajal body formation is regulated by coilin SUMOylation.

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-11 DOI: 10.1242/jcs.263447

Sara K Tucker, Douglas M McLaurin, Michael D Hebert

引用次数: 0

The small ARF-like 2 GTPase TITAN5 is linked with the dynamic regulation of IRON-REGULATED TRANSPORTER 1. 小型类 ARF 2 GTPase TITAN 5 与铁调节运输器 1 的动态调控有关。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-10 DOI: 10.1242/jcs.263645

Inga Mohr, Monique Eutebach, Marie C Knopf, Naima Schommen, Regina Gratz, Kalina Angrand, Lara Genders, Tzvetina Brumbarova, Petra Bauer, Rumen Ivanov

{"title":"The small ARF-like 2 GTPase TITAN5 is linked with the dynamic regulation of IRON-REGULATED TRANSPORTER 1.","authors":"Inga Mohr, Monique Eutebach, Marie C Knopf, Naima Schommen, Regina Gratz, Kalina Angrand, Lara Genders, Tzvetina Brumbarova, Petra Bauer, Rumen Ivanov","doi":"10.1242/jcs.263645","DOIUrl":"10.1242/jcs.263645","url":null,"abstract":"Iron acquisition is crucial for plants. The abundance of IRON-REGULATED TRANSPORTER 1 (IRT1) is controlled through endomembrane trafficking, a process that requires small ARF-like GTPases. Only few components that are involved in the vesicular trafficking of specific cargo are known. Here, we report that the ARF-like GTPase TITAN5 (TTN5) interacts with the large cytoplasmic variable region and protein-regulatory platform of IRT1. Heterozygous ttn5-1 plants can display reduced root iron reductase activity. This activity is needed for iron uptake via IRT1. Fluorescent fusion proteins of TTN5 and IRT1 colocalize at locations where IRT1 sorting and cycling between the plasma membrane and the vacuole are coordinated. TTN5 can also interact with peripheral membrane proteins that are components of the IRT1 regulation machinery, like the trafficking factor SNX1, the C2 domain protein EHB1 and the SEC14-GOLD protein PATL2. Hence, the link between iron acquisition and vesicular trafficking involving a small GTPase of the ARF family opens up the possibility to study the involvement of TTN5 in nutritional cell biology and the endomembrane system.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CAMSAP3 forms dimers via its α-helix domain that directly stabilize non-centrosomal microtubule minus ends. CAMSAP3 通过其 α-helix 结构域形成二聚体，直接稳定非中心粒微管负端。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-09 DOI: 10.1242/jcs.263609

Yuejia Li, Rui Zhang, Jinqi Ren, Wei Chen, Zhengrong Zhou, Honglin Xu, Dong Li, Haisu Cheng, Qi Xie, Wei Ji, Wei Feng, Xin Liang, Wenxiang Meng

{"title":"CAMSAP3 forms dimers via its α-helix domain that directly stabilize non-centrosomal microtubule minus ends.","authors":"Yuejia Li, Rui Zhang, Jinqi Ren, Wei Chen, Zhengrong Zhou, Honglin Xu, Dong Li, Haisu Cheng, Qi Xie, Wei Ji, Wei Feng, Xin Liang, Wenxiang Meng","doi":"10.1242/jcs.263609","DOIUrl":"10.1242/jcs.263609","url":null,"abstract":"Microtubules are vital components of the cytoskeleton. Their plus ends are dynamic and respond to changes in cell morphology, whereas the minus ends are stable and serve a crucial role in microtubule seeding and maintaining spatial organization. In mammalian cells, the calmodulin-regulated spectrin-associated proteins (CAMSAPs), play a key role in directly regulating the dynamics of non-centrosomal microtubules minus ends. However, the molecular mechanisms are not yet fully understood. Our study reveals that CAMSAP3 forms dimers through its C-terminal α-helix; this dimerization not only enhances the microtubule-binding affinity of the CKK domain but also enables the CKK domain to regulate the dynamics of microtubules. Furthermore, CAMSAP3 also specializes in decorating at the minus end of microtubules through the combined action of the microtubule-binding domain (MBD) and the C-terminal α-helix, thereby achieving dynamic regulation of the minus ends of microtubules. These findings are crucial for advancing our understanding and treatment of diseases associated with non-centrosomal microtubules.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142545665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Borg5 restricts contractility and motility in epithelial MDCK cells. Borg5/Cdc42EP1 限制了上皮 MDCK 细胞的收缩性和运动性。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-10 DOI: 10.1242/jcs.261705

David Cohen, Dawn Fernandez, Francisco Lázaro-Diéguez, Beatrix Überheide, Anne Müsch

引用次数: 0

'Iterative Bleaching Extends Multiplexity' facilitates simultaneous identification of all major retinal cell types. 迭代漂白扩展复用技术（IBEX）可同时识别所有主要视网膜细胞类型。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-10 DOI: 10.1242/jcs.263407

Aanandita A Kothurkar, Gregory S Patient, Nicole C L Noel, Aleksandra M Krzywańska, Brittany J Carr, Colin J Chu, Ryan B MacDonald

{"title":"'Iterative Bleaching Extends Multiplexity' facilitates simultaneous identification of all major retinal cell types.","authors":"Aanandita A Kothurkar, Gregory S Patient, Nicole C L Noel, Aleksandra M Krzywańska, Brittany J Carr, Colin J Chu, Ryan B MacDonald","doi":"10.1242/jcs.263407","DOIUrl":"10.1242/jcs.263407","url":null,"abstract":"To understand the multicellular composition of tissues, and how it is altered during development, ageing and/or disease, we must visualise the complete cellular landscape. Currently, this is hindered by our limited ability to combine multiple cellular markers. To overcome this, we adapted a highly multiplexed immunofluorescence (IF) technique called 'Iterative Bleaching Extends Multiplexity' (IBEX) to the zebrafish retina. We optimised fluorescent antibody micro-conjugation to perform sequential rounds of labelling on a single tissue to simultaneously visualise all major retinal cell types with 11 cell-specific antibodies. We further adapted IBEX to be compatible with fluorescent transgenic reporter lines, in situ hybridisation chain reaction (HCR), and whole-mount immunofluorescence (WMIF). We applied IBEX at multiple stages to study the spatial and temporal relationships between glia and neurons during retinal development. Finally, we demonstrate the utility of IBEX across species by testing it on the turquoise killifish (Nothobranchius furzeri) and African clawed frog (Xenopus laevis) to glean large amounts of information from precious tissues. These techniques will revolutionise our ability to visualise multiple cell types in any organism where antibodies are readily available.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proximity labeling reveals interactions necessary to maintain the distinct apical domains of Drosophila photoreceptors. 近距离标记揭示了维持果蝇感光器独特顶端结构所需的相互作用。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-12-01 Epub Date: 2024-12-11 DOI: 10.1242/jcs.262223

Lalitha Sastry, Johnathan Rylee, Simpla Mahato, Andrew C Zelhof

{"title":"Proximity labeling reveals interactions necessary to maintain the distinct apical domains of Drosophila photoreceptors.","authors":"Lalitha Sastry, Johnathan Rylee, Simpla Mahato, Andrew C Zelhof","doi":"10.1242/jcs.262223","DOIUrl":"10.1242/jcs.262223","url":null,"abstract":"Specialized membrane and cortical protein regions are common features of cells and are utilized to isolate differential cellular functions. In Drosophila photoreceptors, the apical membrane domain is defined by two distinct morphological membranes: the rhabdomere microvilli and the stalk membrane. To define the apical cortical protein complexes, we performed proximity labeling screens utilizing the rhabdomeric-specific protein PIP82 as bait. We found that the PIP82 interactome is enriched in actin-binding and cytoskeleton proteins, as well as proteins for cellular trafficking. Analysis of one target, Bifocal, with PIP82 revealed two independent pathways for localization to the rhabdomeric membrane and an additional mechanism of crosstalk between the protein complexes of the rhabdomeric and stalk membranes. The loss of Bifocal, and enhancement in the PIP82, bifocal double mutant, resulted in the additional distribution of Crumbs, an apical stalk membrane protein, to the lateral basal photoreceptor membrane. This phenotype was recapitulated by the knockdown of the catalytic subunit of Protein phosphatase 1, a known interactor with Bifocal. Taken together, these results expand our understanding of the molecular mechanisms underlying the generation of the two distinct photoreceptor apical domains.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-29 DOI: 10.1242/jcs.261920

Yihao Li, Yuwan Zhao, Haining Zhang, Peiwei Liu, Haiyun Ren

{"title":"The Ras-related nuclear GTPase RAN1 ensures pollen size and tube growth via maintaining actin cytoskeleton.","authors":"Yihao Li, Yuwan Zhao, Haining Zhang, Peiwei Liu, Haiyun Ren","doi":"10.1242/jcs.261920","DOIUrl":"https://doi.org/10.1242/jcs.261920","url":null,"abstract":"Controlling organ size in plants is a complex biological process influenced by various factors, including gene expression, genome ploidy, and environmental conditions. Despite its importance for plant growth and development, the mechanisms underlying organ size regulation remain unknown. Here, we investigated the role of RAN1, a member of the Ras-related nuclear GTPases family, in regulating pollen size. A RAN1 knockdown mutant (ran1-1) exhibited a significant reduction in pollen size, accompanied by impaired germination and reduced pollen tube growth. RAN1 mutation caused disruptions in actin filament organization such as aberrant structure of actin collar due to the dysregulation of actin-binding proteins expression. Furthermore, we identified the transcription activator SHB1 (SHORT HYPOCOTYL UNDER BLUE1), whose mutation showed similar but milder phenotypes in pollens compared to ran1-1. Genetic evidence suggested SHB1 acts downstream of RAN1. Transient expression assays in leaves showed that SHB1 was largely retained in the cytoplasm of the ran1-1 mutant, potentially affecting the expression of actin-binding proteins. These findings highlight the pivotal role of RAN1 in modulating pollen size and development, providing valuable insights into cell size regulation.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142750693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0