Journal of cell science最新文献_第10页

Bi-directional regulation between inflammation and stem cells in the respiratory tract. 呼吸道炎症和干细胞之间的双向调节。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-01 Epub Date: 2024-11-07 DOI: 10.1242/jcs.263413

Jinwook Choi, Jakub Chudziak, Joo-Hyeon Lee

{"title":"Bi-directional regulation between inflammation and stem cells in the respiratory tract.","authors":"Jinwook Choi, Jakub Chudziak, Joo-Hyeon Lee","doi":"10.1242/jcs.263413","DOIUrl":"10.1242/jcs.263413","url":null,"abstract":"Inflammation plays a crucial role in tissue injury, repair and disease, orchestrating a complex interplay of immune responses and cellular processes. Recent studies have uncovered the intricate connection between inflammation and stem cell dynamics, shedding light on the central role of stem cells in tissue regeneration. This Review highlights the significance of inflammation in shaping epithelial stem cell dynamics and its implications for tissue repair, regeneration and aging. We explore the multifaceted interactions between inflammation and stem cells, focusing on how inflammatory signals affect stem cell behavior and fate as well as the remodeling of their niche in the respiratory tract. We also discuss the concept of 'inflammatory memory' in epithelial stem cells, where prior inflammatory stimuli endow these cells with enhanced regenerative potential and confer long-lasting protective mechanisms for maintaining tissue integrity and function. Furthermore, we review the impact of cell senescence induced by inflammation on tissue regeneration and aging, delving into the molecular mechanisms underlying the modulation of signaling pathways, epigenetic modifications and cellular crosstalk. Understanding these dynamic processes not only deepens our knowledge of tissue homeostasis and repair but also holds profound implications for regenerative medicine strategies aimed at preventing pulmonary diseases.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":"137 21","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11574357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A single-particle analysis method for detecting membrane remodelling and curvature sensing. 检测膜重塑和曲率感应的单颗粒分析方法。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-01 Epub Date: 2024-11-07 DOI: 10.1242/jcs.263533

Adeline Colussi, Leonardo Almeida-Souza, Harvey T McMahon

{"title":"A single-particle analysis method for detecting membrane remodelling and curvature sensing.","authors":"Adeline Colussi, Leonardo Almeida-Souza, Harvey T McMahon","doi":"10.1242/jcs.263533","DOIUrl":"10.1242/jcs.263533","url":null,"abstract":"In biology, shape and function are related. Therefore, it is important to understand how membrane shape is generated, stabilised and sensed by proteins and how this relates to organelle function. Here, we present an assay that can detect curvature preference and membrane remodelling with free-floating liposomes using protein concentrations in physiologically relevant ranges. The assay reproduced known curvature preferences of BAR domains and allowed the discovery of high-curvature preference for the PH domain of AKT and the FYVE domain of HRS (also known as HGS). In addition, our method reproduced the membrane vesiculation activity of the ENTH domain of epsin-1 (EPN1) and showed similar activity for the ANTH domains of PiCALM and Hip1R. Finally, we found that the curvature sensitivity of the N-BAR domain of endophilin inversely correlates to membrane charge and that deletion of its N-terminal amphipathic helix increased its curvature specificity. Thus, our method is a generally applicable qualitative method for assessing membrane curvature sensing and remodelling by proteins.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11574359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142347441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

prominin-1-null Xenopus laevis develop subretinal drusenoid-like deposits, cone-rod dystrophy and RPE atrophy. Prominin-1缺失的爪蟾会出现视网膜下类风湿沉积、锥杆营养不良和 RPE 萎缩。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-01 Epub Date: 2024-11-12 DOI: 10.1242/jcs.262298

Brittany J Carr, Dominic Skitsko, Linnea M Kriese, Jun Song, Zixuan Li, Myeong Jin Ju, Orson L Moritz

{"title":"prominin-1-null Xenopus laevis develop subretinal drusenoid-like deposits, cone-rod dystrophy and RPE atrophy.","authors":"Brittany J Carr, Dominic Skitsko, Linnea M Kriese, Jun Song, Zixuan Li, Myeong Jin Ju, Orson L Moritz","doi":"10.1242/jcs.262298","DOIUrl":"10.1242/jcs.262298","url":null,"abstract":"Prominin-1 (PROM1) variants are associated with inherited, non-syndromic vision loss. We used CRISPR/Cas9 to induce prom1-null mutations in Xenopus laevis and then tracked retinal disease progression from the ages of 6 weeks to 3 years. We found that prom1-null-associated retinal degeneration in frogs was age-dependent and involved retinal pigment epithelium (RPE) dysfunction preceding photoreceptor degeneration. Before photoreceptor degeneration occurred, aging prom1-null frogs developed larger and increasing numbers of cellular debris deposits in the subretinal space and outer segment layer, which resembled subretinal drusenoid deposits (SDDs) in their location, histology and representation as seen by color fundus photography and optical coherence tomography (OCT). Evidence for an RPE origin of these deposits included infiltration of pigment granules into the deposits, thinning of the RPE as measured by OCT, and RPE disorganization as measured by histology and OCT. The appearance and accumulation of SDD-like deposits and RPE thinning and disorganization in our animal model suggests an underlying disease mechanism for prom1-null-mediated blindness that involves death and dysfunction of the RPE preceding photoreceptor degeneration, instead of direct effects upon photoreceptor outer segment morphogenesis, as was previously hypothesized.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A microtubule stability switch alters isolated vascular smooth muscle Ca2+ flux in response to matrix rigidity. 微管稳定性开关可改变离体血管平滑肌钙通量，以应对基质刚性。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-01 Epub Date: 2024-11-12 DOI: 10.1242/jcs.262310

Robert T Johnson, Finn Wostear, Reesha Solanki, Oliver Steward, Alice Bradford, Christopher Morris, Stefan Bidula, Derek T Warren

{"title":"A microtubule stability switch alters isolated vascular smooth muscle Ca2+ flux in response to matrix rigidity.","authors":"Robert T Johnson, Finn Wostear, Reesha Solanki, Oliver Steward, Alice Bradford, Christopher Morris, Stefan Bidula, Derek T Warren","doi":"10.1242/jcs.262310","DOIUrl":"10.1242/jcs.262310","url":null,"abstract":"During ageing, the extracellular matrix of the aortic wall becomes more rigid. In response, vascular smooth muscle cells (VSMCs) generate enhanced contractile forces. Our previous findings demonstrate that VSMC volume is enhanced in response to increased matrix rigidity, but our understanding of the mechanisms regulating this process remain incomplete. In this study, we show that microtubule stability in VSMCs is reduced in response to enhanced matrix rigidity via Piezo1-mediated Ca2+ influx. Moreover, VSMC volume and Ca2+ flux is regulated by microtubule dynamics; microtubule-stabilising agents reduced both VSMC volume and Ca2+ flux on rigid hydrogels, whereas microtubule-destabilising agents increased VSMC volume and Ca2+ flux on pliable hydrogels. Finally, we show that disruption of the microtubule deacetylase HDAC6 uncoupled these processes and increased α-tubulin acetylation on K40, VSMC volume and Ca2+ flux on pliable hydrogels, but did not alter VSMC microtubule stability. These findings uncover a microtubule stability switch that controls VSMC volume by regulating Ca2+ flux. Taken together, these data demonstrate that manipulation of microtubule stability can modify VSMC response to matrix stiffness.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142288099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An improved tetracycline-inducible expression system for fission yeast. 改进的裂殖酵母四环素诱导表达系统。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-01 DOI: 10.1242/jcs.263404

Xiao-Hui Lyu, Yu-Sheng Yang, Zhao-Qian Pan, Shao-Kai Ning, Fang Suo, Li-Lin Du

{"title":"An improved tetracycline-inducible expression system for fission yeast.","authors":"Xiao-Hui Lyu, Yu-Sheng Yang, Zhao-Qian Pan, Shao-Kai Ning, Fang Suo, Li-Lin Du","doi":"10.1242/jcs.263404","DOIUrl":"10.1242/jcs.263404","url":null,"abstract":"The ability to manipulate gene expression is valuable for elucidating gene function. In the fission yeast Schizosaccharomyces pombe, the most widely used regulatable expression system is the nmt1 promoter and its two attenuated variants. However, these promoters have limitations, including a long lag, incompatibility with rich media and unsuitability for non-dividing cells. Here, we present a tetracycline-inducible system free of these shortcomings. Our system features the enotetS promoter, which achieves a similar induced level and a higher induction ratio compared to the nmt1 promoter, without exhibiting a lag. Additionally, our system includes four weakened enotetS variants, offering an expression range similar to that of the nmt1 series promoters but with more intermediate levels. To enhance usability, each promoter is combined with a Tet-repressor-expressing cassette in an integration plasmid. Importantly, our system can be used in non-dividing cells, enabling the development of a synchronous meiosis induction method with high spore viability. Moreover, our system allows for the shutdown of gene expression and the generation of conditional loss-of-function mutants. This system provides a versatile and powerful tool for manipulating gene expression in fission yeast.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142347442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disordered hinge regions of the AP-3 adaptor complex promote vesicle budding from the late Golgi in yeast. AP-3适配体复合物的无序铰链区可促进酵母中囊泡从晚期高尔基体出芽。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-01 Epub Date: 2024-11-08 DOI: 10.1242/jcs.262234

Mitchell Leih, Rachael L Plemel, Matt West, Cortney G Angers, Alexey J Merz, Greg Odorizzi

{"title":"Disordered hinge regions of the AP-3 adaptor complex promote vesicle budding from the late Golgi in yeast.","authors":"Mitchell Leih, Rachael L Plemel, Matt West, Cortney G Angers, Alexey J Merz, Greg Odorizzi","doi":"10.1242/jcs.262234","DOIUrl":"10.1242/jcs.262234","url":null,"abstract":"Vesicles bud from maturing Golgi cisternae in a programmed sequence. Budding is mediated by adaptors that recruit cargoes and facilitate vesicle biogenesis. In Saccharomyces cerevisiae, the AP-3 adaptor complex directs cargoes from the Golgi to the lysosomal vacuole. The AP-3 core consists of small and medium subunits complexed with two non-identical large subunits, β3 (Apl6) and δ (Apl5). The C-termini of β3 and δ were thought to be flexible hinges linking the core to ear domains that bind accessory proteins involved in vesicular transport. We found by computational modeling that the yeast β3 and δ hinges are intrinsically disordered and lack folded ear domains. When either hinge is truncated, AP-3 is recruited to the Golgi, but vesicle budding is impaired and cargoes normally sorted into the AP-3 pathway are mistargeted. This budding deficiency causes AP-3 to accumulate on ring-like Golgi structures adjacent to GGA adaptors that, in wild-type cells, bud vesicles downstream of AP-3 during Golgi maturation. Thus, each of the disordered hinges of yeast AP-3 has a crucial role in mediating transport vesicle formation at the Golgi.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11574352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142347444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Graham A. Dunn (1944-2024) - a pioneer of cell migration analysis. 格雷厄姆-邓恩（Graham A. Dunn，1944-2024 年）--细胞迁移分析的先驱。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-01 Epub Date: 2024-11-07 DOI: 10.1242/jcs.263606

Gareth E Jones, Alexander Bershadsky

引用次数: 0

Spatiotemporal regulation of organelle transport by spindle position checkpoint kinase Kin4. 纺锤体位置检查点激酶Kin4对细胞器运输的时空调控

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-01 Epub Date: 2024-11-13 DOI: 10.1242/jcs.261948

Lakhan Ekal, Abdulaziz M S Alqahtani, Kathryn R Ayscough, Ewald H Hettema

引用次数: 0

Making the most of bioimaging data through interdisciplinary interactions. 通过跨学科互动充分利用生物成像数据。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-10-15 Epub Date: 2024-10-23 DOI: 10.1242/jcs.262139

Virginie Uhlmann, Matthew Hartley, Josh Moore, Erin Weisbart, Assaf Zaritsky

引用次数: 0

cellPLATO - an unsupervised method for identifying cell behaviour in heterogeneous cell trajectory data. cellPLATO：在异质细胞轨迹数据中识别细胞行为的无监督方法。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-10-15 Epub Date: 2024-06-12 DOI: 10.1242/jcs.261887

Michael J Shannon, Shira E Eisman, Alan R Lowe, Tyler F W Sloan, Emily M Mace

{"title":"cellPLATO - an unsupervised method for identifying cell behaviour in heterogeneous cell trajectory data.","authors":"Michael J Shannon, Shira E Eisman, Alan R Lowe, Tyler F W Sloan, Emily M Mace","doi":"10.1242/jcs.261887","DOIUrl":"10.1242/jcs.261887","url":null,"abstract":"Advances in imaging, segmentation and tracking have led to the routine generation of large and complex microscopy datasets. New tools are required to process this 'phenomics' type data. Here, we present 'Cell PLasticity Analysis Tool' (cellPLATO), a Python-based analysis software designed for measurement and classification of cell behaviours based on clustering features of cell morphology and motility. Used after segmentation and tracking, the tool extracts features from each cell per timepoint, using them to segregate cells into dimensionally reduced behavioural subtypes. Resultant cell tracks describe a 'behavioural ID' at each timepoint, and similarity analysis allows the grouping of behavioural sequences into discrete trajectories with assigned IDs. Here, we use cellPLATO to investigate the role of IL-15 in modulating human natural killer (NK) cell migration on ICAM-1 or VCAM-1. We find eight behavioural subsets of NK cells based on their shape and migration dynamics between single timepoints, and four trajectories based on sequences of these behaviours over time. Therefore, by using cellPLATO, we show that IL-15 increases plasticity between cell migration behaviours and that different integrin ligands induce different forms of NK cell migration.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11213520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140911834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0