Adherens junctions limit septate junction length in Drosophila midgut enterocytes but are not required for polarity.

IF 3.3 3区 生物学 Q3 CELL BIOLOGY
Cátia A Carvalho, Mihoko A Tame, Daniel St Johnston
{"title":"Adherens junctions limit septate junction length in Drosophila midgut enterocytes but are not required for polarity.","authors":"Cátia A Carvalho, Mihoko A Tame, Daniel St Johnston","doi":"10.1242/jcs.263644","DOIUrl":null,"url":null,"abstract":"<p><p>Adherens junctions formed by E-cadherin adhesion complexes play central roles in the organisation and apical-basal polarisation of both mammalian and insect epithelia. Here we investigate the function of the components of the E-cadherin adhesion complex in the Drosophila midgut epithelium, which establishes polarity by a different mechanism from other fly epithelia and has an inverted junctional arrangement, in which the adherens junctions lie below the septate junctions. Unlike other epithelial tissues, loss of E-cadherin, Armadillo (β-catenin) or ⍺-catenin has no effect on the polarity or organisation of the adult midgut epithelium. This is not due to redundancy with other cadherins, as enterocytes lacking E-cadherin, N-cadherin and CadN2 still polarise normally. However, E-cadherin (shg) and armadillo mutants have expanded septate junction domains and shorter lateral domains below the septate junctions, indicating that E-cadherin adhesion complexes limit the basal extent of the septate junctions. Thus, Cadherin-mediated adhesion is dispensable for apical-basal polarity and epithelial organisation in the Drosophila midgut, in contrast to all other epithelia that have been studied so far, but it is required to define the size of the septate junctions and cell height.</p>","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3000,"publicationDate":"2025-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of cell science","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1242/jcs.263644","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Adherens junctions formed by E-cadherin adhesion complexes play central roles in the organisation and apical-basal polarisation of both mammalian and insect epithelia. Here we investigate the function of the components of the E-cadherin adhesion complex in the Drosophila midgut epithelium, which establishes polarity by a different mechanism from other fly epithelia and has an inverted junctional arrangement, in which the adherens junctions lie below the septate junctions. Unlike other epithelial tissues, loss of E-cadherin, Armadillo (β-catenin) or ⍺-catenin has no effect on the polarity or organisation of the adult midgut epithelium. This is not due to redundancy with other cadherins, as enterocytes lacking E-cadherin, N-cadherin and CadN2 still polarise normally. However, E-cadherin (shg) and armadillo mutants have expanded septate junction domains and shorter lateral domains below the septate junctions, indicating that E-cadherin adhesion complexes limit the basal extent of the septate junctions. Thus, Cadherin-mediated adhesion is dispensable for apical-basal polarity and epithelial organisation in the Drosophila midgut, in contrast to all other epithelia that have been studied so far, but it is required to define the size of the septate junctions and cell height.

粘附连接限制了果蝇中肠肠细胞的分离连接长度,但不是极性所必需的。
由e -钙粘蛋白黏附复合物形成的粘附连接在哺乳动物和昆虫上皮的组织和顶基极化中起着核心作用。在这里,我们研究了e -钙粘蛋白粘附复合物在果蝇中肠上皮中的功能,该细胞通过不同于其他果蝇上皮的机制建立极性,并具有倒置的连接排列,其中粘附连接位于分隔连接下方。与其他上皮组织不同,E-cadherin、Armadillo (β-catenin)或-catenin的缺失对成年中肠上皮的极性或组织没有影响。这不是由于与其他钙粘蛋白的冗余,因为缺乏E-cadherin, N-cadherin和CadN2的肠细胞仍然正常极化。然而,E-cadherin (shg)和犰狳突变体具有扩大的间隔连接域和更短的间隔连接域,表明E-cadherin粘附复合物限制了间隔连接的基底范围。因此,与迄今为止研究的所有其他上皮细胞相比,钙粘蛋白介导的粘附对于果蝇中肠的顶基极性和上皮组织是必不可少的,但它需要确定分隔连接的大小和细胞高度。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of cell science
Journal of cell science 生物-细胞生物学
CiteScore
7.30
自引率
2.50%
发文量
393
审稿时长
1.4 months
期刊介绍: Journal of Cell Science publishes cutting-edge science, encompassing all aspects of cell biology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信