Journal of cell science最新文献_第2页

Removing barriers to advanced imaging and machine learning-based analysis. 消除高级成像和基于机器学习的分析的障碍。

IF 3.9 3区生物学

Journal of cell science Pub Date : 2026-11-01 Epub Date: 2026-08-12 DOI: 10.1242/jcs.264705

Jodie R Malcolm, Stuart Lacy, Andrea Papaleo, Le Liu, Suleiman H Kwairanga, Graeme Park, Joanne Marrison, Olympia Physouni, Richard Kasprowicz, Kerry Hallbrook, Richard Williams, Kildare Miranda, Teng-Leong Chew, Caron A Jacobs, Mahmoud Bukar Maina, Ana Paula C A Lima, Jeremy C Mottram, Beth A Cimini, Ben Powell, Peter O'Toole, Laura Wiggins, William J Brackenbury

{"title":"Removing barriers to advanced imaging and machine learning-based analysis.","authors":"Jodie R Malcolm, Stuart Lacy, Andrea Papaleo, Le Liu, Suleiman H Kwairanga, Graeme Park, Joanne Marrison, Olympia Physouni, Richard Kasprowicz, Kerry Hallbrook, Richard Williams, Kildare Miranda, Teng-Leong Chew, Caron A Jacobs, Mahmoud Bukar Maina, Ana Paula C A Lima, Jeremy C Mottram, Beth A Cimini, Ben Powell, Peter O'Toole, Laura Wiggins, William J Brackenbury","doi":"10.1242/jcs.264705","DOIUrl":"10.1242/jcs.264705","url":null,"abstract":"Global and community-driven initiatives have recently achieved considerable success in overcoming key challenges that hinder the widespread adoption of advanced microscopy and bioimage analysis tools in under-resourced settings. To build upon this progress, we held a workshop in May 2025 at the University of York, UK to address the needs and barriers associated with implementing time-lapse imaging and machine learning-based phenotyping in low-resource research environments. We focussed on identifying the specific challenges faced by the existing networks represented at the meeting, emphasising how integrating combined imaging hardware and machine learning-based approaches can solve these problems. This article summarises the key observations and actionable strategies made at the workshop. These proposed steps aim to significantly increase the dissemination and uptake of these powerful technologies to advance biological research in low-resource settings globally.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":"139 21","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7619436/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148712350","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

From the flagellum attachment zone to the tripartite attachment complex - widespread cytoskeletal engagement of T. brucei KMP11. 从FAZ到TAC：布鲁氏霉KMP11广泛的细胞骨架接合。

IF 3.9 3区生物学

Journal of cell science Pub Date : 2026-11-01 Epub Date: 2026-08-19 DOI: 10.1242/jcs.264898

Salome Aeschlimann, Clirim Jetishi, Caroline E Dewar, Philip Stettler, Markus Gerber, Silke Oeljeklaus, Bettina Warscheid, Torsten Ochsenreiter, André Schneider

{"title":"From the flagellum attachment zone to the tripartite attachment complex - widespread cytoskeletal engagement of T. brucei KMP11.","authors":"Salome Aeschlimann, Clirim Jetishi, Caroline E Dewar, Philip Stettler, Markus Gerber, Silke Oeljeklaus, Bettina Warscheid, Torsten Ochsenreiter, André Schneider","doi":"10.1242/jcs.264898","DOIUrl":"10.1242/jcs.264898","url":null,"abstract":"KMP11 is a conserved kinetoplastid protein yet its precise localization and function remained unclear. Using immunofluorescence, ultrastructure expansion microscopy (U-ExM), and proteomics, we comprehensively analyzed KMP11 in procyclic Trypanosoma brucei. Besides confirming localization to the flagellum attachment zone (FAZ), we identify previously unknown localizations at the paraflagellar rod (PFR), flagellar connector (FC), microtubule quartet (MtQ) and flagellar pocket collar/hook complex (FPC/HC) region. We further show that the previously described basal body localization corresponds to the tripartite attachment complex (TAC). Proteomic analyses identify multiple cytoskeletal interactors, including the TAC component p197. Immunoprecipitation and U-ExM analyses demonstrate that KMP11 associates with the central α-helical domain of p197 from both T. brucei and Trypanosoma cruzi. Furthermore, KMP11 interactors overall display significantly elevated α-helical content. Together, our findings establish KMP11 as a component of a broad cytoskeletal interaction network in trypanosomes and we speculate that KMP11 preferentially associates with α-helical proteins.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13539426/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148390466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 3D printable device for non-invasive longitudinal neuroimaging in adult zebrafish. 一种用于成年斑马鱼非侵入性纵向神经成像的3D可打印装置。

IF 3.9 3区生物学

Journal of cell science Pub Date : 2026-11-01 Epub Date: 2026-10-20 DOI: 10.1242/jcs.264788

Elizabeth M Haynes, Corey Steinhauser, Alexandra M Fister, Robert Swader, George Petry, Tyler K Ulland, Kevin W Eliceiri

{"title":"A 3D printable device for non-invasive longitudinal neuroimaging in adult zebrafish.","authors":"Elizabeth M Haynes, Corey Steinhauser, Alexandra M Fister, Robert Swader, George Petry, Tyler K Ulland, Kevin W Eliceiri","doi":"10.1242/jcs.264788","DOIUrl":"10.1242/jcs.264788","url":null,"abstract":"Zebrafish are a powerful model for imaging studies of development and neurobiology. However, most studies have focused on developing zebrafish because of the technical and biological challenges of imaging adult stages. These include increased tissue opacity and illumination depth limitations, and difficulty maintaining life support and anesthesia in a 2-4 cm-long fish. There are currently limited tools for intravital imaging of the adult zebrafish brain. The ability to image the brain in the same individual repeatedly without physical damage would facilitate the use of zebrafish to study aging and neurodegenerative disease. We designed and applied a 3D printable device for non-invasive, repeatable multiphoton neural imaging of genetically non-pigmented adult zebrafish across multiple ages and sizes. Animals successfully recovered after multi-hour imaging sessions and could be imaged repeatedly over periods of weeks to months. We show the utility of this approach through imaging the dynamic cells of the neuroimmune system, as well as radial glial cells and oligodendrocytes. This technique could be widely used and beneficial for other cell-scale neuroimaging studies in adult fish.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148818557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Septins as novel regulators of podosome cluster organization and dynamics in human immature dendritic cells. septin作为人类未成熟树突状细胞足小体簇组织和动力学的新调节剂。

IF 3.9 3区生物学

Journal of cell science Pub Date : 2026-10-16 DOI: 10.1242/jcs.264945

Koen van den Dries, Rozanne Straten, Alessandra Cambi

{"title":"Septins as novel regulators of podosome cluster organization and dynamics in human immature dendritic cells.","authors":"Koen van den Dries, Rozanne Straten, Alessandra Cambi","doi":"10.1242/jcs.264945","DOIUrl":"https://doi.org/10.1242/jcs.264945","url":null,"abstract":"Podosomes are actin-rich adhesions that enable cells to probe their environment, migrate across tissue boundaries, and degrade extracellular matrix. They are often organized in clusters, where their spatial organization is governed by complex actin networks, including a vinculin-associated ventral network and a myosin IIA-associated dorsal network. The molecular composition and mechanisms coordinating these networks, however, remain incompletely understood. Here, we identify septins as novel constituents of the podosome network in human immature dendritic cells. Using super-resolution microscopy, we show that septin 2, 7 and 9 are excluded from the actin core and do not colocalize with the canonical podosome ring. Instead, septins localize to the dorsal actin network that interconnects individual podosomes. Disruption of the septin network does not prevent podosome formation but alters the spatiotemporal organization of the podosome actin network. Furthermore, although septins and myosin IIA do not directly colocalize, septin perturbation leads to axial mislocalization and reduced activity of myosin IIA. Together, our findings identify septins as novel regulators of podosome cluster organization, adding an additional layer of complexity to the cytoskeletal coordination of these structures.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149193986","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Two-step regulation of bouquet formation by mating pheromone signaling and Pat1 activity reduction during fission yeast meiosis. 裂变酵母减数分裂过程中交配信息素信号和Pat1活性降低对花束形成的两步调控。

IF 3.9 3区生物学

Journal of cell science Pub Date : 2026-10-14 DOI: 10.1242/jcs.264805

Alimul Islam, Shinnosuke Kawai, Ayumu Yamamoto

{"title":"Two-step regulation of bouquet formation by mating pheromone signaling and Pat1 activity reduction during fission yeast meiosis.","authors":"Alimul Islam, Shinnosuke Kawai, Ayumu Yamamoto","doi":"10.1242/jcs.264805","DOIUrl":"https://doi.org/10.1242/jcs.264805","url":null,"abstract":"Upon meiosis initiation in the fission yeast Schizosaccharomyces pombe, centromeres detach from the spindle pole body (SPB), while telomeres cluster at the SPB, forming the bouquet, which promotes homologous chromosome pairing. Bouquet formation is regulated by mating pheromone signaling and Pat1 kinase, a central regulator of meiosis. Centromere detachment also requires telomere clustering-dependent SPB recruitment of the telomere component Taz1. However, the precise underlying regulatory mechanisms remain unclear. Here, using a haploid cell system that allows synchronous meiosis induction, we show that mating pheromone signaling induces transient bouquet formation, whereas bouquet maintenance additionally requires Pat1 activity reduction, which normally occurs after conjugation. Furthermore, impairment of telomere clustering inhibits centromere detachment regardless of Pat1 activity reduction, whereas SPB-recruited Taz1 restores centromere detachment only when Pat1 activity is reduced. Thus, bouquet formation proceeds in two steps: a transient establishment step dependent on mating pheromone signaling, followed by a maintenance step requiring Pat1 activity reduction and SPB recruitment of Taz1. We propose that this regulation enables rapid cell cycle resumption upon nutrient replenishment in unconjugated cells, while ensuring meiotic commitment in conjugated cells.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149182039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Visualisation of the regulation of CDK/cyclin complexes by p21 (CDKN1A) in living cells using FLIM-FRET. 利用flm - fret可视化活细胞中p21 （CDKN1A）对CDK/cyclin复合物的调控。

IF 3.9 3区生物学

Journal of cell science Pub Date : 2026-10-14 DOI: 10.1242/jcs.265097

Christian Melle, Birgit Hoffmann, Annett Wiesenburg, Christoph Biskup

{"title":"Visualisation of the regulation of CDK/cyclin complexes by p21 (CDKN1A) in living cells using FLIM-FRET.","authors":"Christian Melle, Birgit Hoffmann, Annett Wiesenburg, Christoph Biskup","doi":"10.1242/jcs.265097","DOIUrl":"https://doi.org/10.1242/jcs.265097","url":null,"abstract":"The cell cycle is controlled in G1-phase by activities of three cyclin-dependent kinases (CDKs), CDK4/6 and CDK2, and their regulatory partners, cyclin D and cyclin E, respectively. p21 (CDKN1A), a member of the KIP/CIP family, is involved in regulation of CDK/cyclin complexes. In the present study, we analysed the impact of p21 on CDK/cyclin complex formation in living cells by fluorescence lifetime imaging microscopy (FLIM)-based Förster resonance energy transfer (FRET) measurements. Depending on the additional expression of p21, both CDK4/cyclin D3 and CDK2/cyclin E1 interactions have been favoured reflected by increased FRET efficiencies (E). Furthermore, post-translational modifications (PTM) as well as the redox status of p21 can regulate the impact on CDK/cyclin complexes. We show that phosphorylation-mimicking mutants of p21 seem to be able to affect the CDK/cyclin protein-protein interactions (PPIs), and that the reduced p21C41S variant decreased binding between CDK4 and cyclin D3. In contrast, p21C41S increased the CDK2/cyclin E1 interaction such as wild-type p21. Hence, wild-type p21 and its specific variants have an impact on the formation of distinct CDK/cyclin complexes under physiological conditions.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149182054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FRET imaging of ERK activity in an orthotopic xenograft model of patient-derived pancreatic ductal adenocarcinoma using imaging window. 利用成像窗对患者源性胰腺导管腺癌原位异种移植模型中ERK活性的FRET成像。

IF 3.9 3区生物学

Journal of cell science Pub Date : 2026-10-09 DOI: 10.1242/jcs.265075

Houssam Al-Koussa, Daisuke Taniguchi, Masafumi Kuroda, Yukino Inomata, Ye Huaze, Akihito Machinaga, Nobuyuki Kakiuchi, Seishi Ogawa, Hiroshi Seno, Shigeki Higashiyama, Michiyuki Matsuda, Toru Hiratsuka

{"title":"FRET imaging of ERK activity in an orthotopic xenograft model of patient-derived pancreatic ductal adenocarcinoma using imaging window.","authors":"Houssam Al-Koussa, Daisuke Taniguchi, Masafumi Kuroda, Yukino Inomata, Ye Huaze, Akihito Machinaga, Nobuyuki Kakiuchi, Seishi Ogawa, Hiroshi Seno, Shigeki Higashiyama, Michiyuki Matsuda, Toru Hiratsuka","doi":"10.1242/jcs.265075","DOIUrl":"https://doi.org/10.1242/jcs.265075","url":null,"abstract":"Intravital imaging of cancer xenograft models enables the observation of cells in their tissue microenvironment. Application of molecular activity probes to intravital imaging, however, often poses technical difficulties. Here we sought to visualize Extracellular signal-regulated kinase (ERK) activity in an orthotopic xenograft model of pancreatic cancer. Our custom-made pancreatic imaging window (PIW) enabled long-term tracking (> 2 weeks) of cancer xenografts showing heterogeneous growth potential. Our validation approach of Förster resonance energy transfer (FRET) imaging by a kinase-dead mutant ERK biosensor suggested the local pH may affect the ratiometric interpretation of FRET efficiency. Nevertheless, our FRET analysis allowed to detect pharmacological inhibition of ERK activity, which supports the validity of the experimental model to dissect heterogeneous drug responses of cancer cells in their microenvironment. Of note, we found that collagen signal was negatively correlated with local ERK activity heterogeneity, highlighting the benefit of our imaging system to observe the intricate pharmacodynamics modified by fibrosis. Our intravital live imaging platform paves the way for a novel pre-clinical model that links the molecular dynamics to the living tissue environment of pancreatic cancer.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149136778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A phosphoinositide and RAB switch controls early macropinocytosis. 磷酸肌肽和RAB开关控制早期巨噬细胞增多症。

IF 3.9 3区生物学

Journal of cell science Pub Date : 2026-10-09 DOI: 10.1242/jcs.265349

Hélène Spangenberg, Emilie Løgith Moe, Øyvind Ødegård Fougner, Marte Sneeggen, Meng Pan, Kia Wee Tan, Maria Mateo Tortola, Camila Valenzuela, Yuen-Yan Chang, Harald Stenmark, Camilla Raiborg, Kay Oliver Schink

{"title":"A phosphoinositide and RAB switch controls early macropinocytosis.","authors":"Hélène Spangenberg, Emilie Løgith Moe, Øyvind Ødegård Fougner, Marte Sneeggen, Meng Pan, Kia Wee Tan, Maria Mateo Tortola, Camila Valenzuela, Yuen-Yan Chang, Harald Stenmark, Camilla Raiborg, Kay Oliver Schink","doi":"10.1242/jcs.265349","DOIUrl":"https://doi.org/10.1242/jcs.265349","url":null,"abstract":"Macropinocytosis is a non-selective endocytic process by which cells take up large amounts of extracellular fluid into giant vesicles known as macropinosomes. This mechanism is used by immune cells to sample the surroundings for antigens and can be exploited by cancer cells for nutrient uptake. What determines macropinosome fate after internalization is largely unknown. Here we investigate the role of the phosphatidylinositol 3-kinase VPS34/PIK3C3 and its product phosphatidylinositol 3-phosphate (PtdIns3P) in macropinosome fate determination. VPS34 inhibition reduced successful macropinosome maturation and fluid-phase uptake and prevented recruitment of early endosomal factors, including the small GTPase RAB5A and its effectors, to forming macropinosomes. Instead, forming macropinosomes under VPS34 inhibition accumulated regulators of endocytic recycling, including RAB8A, RAB10, RAB11A, and PtdIns4P, which led to fusion of macropinosomes with the plasma membrane. Whereas RAB5A was critical for establishment of endosomal identity, macropinosome fusion with the plasma membrane depended on RAB8A. Thus, macropinosome maturation is regulated by a VPS34-dependent phosphoinositide and RAB transition that determines whether newly formed macropinosomes acquire endosomal identity or recycle to the plasma membrane.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149136806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BRD8 deficiency leads to progressive impairment of spermatogenesis in mice. BRD8缺乏导致小鼠精子发生的进行性损害。

IF 3.9 3区生物学

Journal of cell science Pub Date : 2026-10-08 DOI: 10.1242/jcs.265013

Saya Nakagawa, Manabu Ozawa, Teh-Wei Wang, Tsuneo Ikenoue, Yumiko Isobe, Eigo Shimizu, Makoto Nakanishi, Seiya Imoto, Yoichi Furukawa, Kiyoshi Yamaguchi

{"title":"BRD8 deficiency leads to progressive impairment of spermatogenesis in mice.","authors":"Saya Nakagawa, Manabu Ozawa, Teh-Wei Wang, Tsuneo Ikenoue, Yumiko Isobe, Eigo Shimizu, Makoto Nakanishi, Seiya Imoto, Yoichi Furukawa, Kiyoshi Yamaguchi","doi":"10.1242/jcs.265013","DOIUrl":"https://doi.org/10.1242/jcs.265013","url":null,"abstract":"BRD8, a member of the bromodomain-containing protein family, is a chromatin-associated protein that recognizes acetylated histones and recruits regulatory factors to modulate gene expression. Despite extensive studies of BRD8 in tumor biology, its physiological roles in normal tissues remain largely unknown. Given its enriched expression in the testis, we investigated the role of Brd8 in male reproduction using a germ cell-specific Brd8 knockout mouse model. Brd8 deficiency resulted in a marked reduction in testicular size and sperm counts, accompanied by severe defects in germ cell differentiation. Immunofluorescence staining for germ cell markers demonstrated progressive spermatogenic defects, with a reduction in spermatids followed by declines in spermatocytes and spermatogonia at later ages. Single-cell transcriptome analysis of Brd8-knockout testes revealed transcriptional alterations in spermatogonia and spermatocytes, including dysregulation of translation-related pathways and reduced expression of genes involved in meiotic progression. In addition, VEGF-related signaling within the endothelial compartment was reduced in Brd8-knockout testes, suggesting secondary remodeling of the testicular microenvironment associated with progressive germ cell dysfunction. Our findings provide new insights into the role of BRD8, an epigenetic reader, in male fertility.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149136850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A distinct interphase microtubule state marks host cell permissiveness to Chlamydia pneumoniae entry. 明显的间期微管状态标志着宿主细胞允许肺炎衣原体进入。

IF 3.9 3区生物学

Journal of cell science Pub Date : 2026-10-07 DOI: 10.1242/jcs.265369

Katharina Schenk, Johannes Hegemann, Ursula Fleig

{"title":"A distinct interphase microtubule state marks host cell permissiveness to Chlamydia pneumoniae entry.","authors":"Katharina Schenk, Johannes Hegemann, Ursula Fleig","doi":"10.1242/jcs.265369","DOIUrl":"https://doi.org/10.1242/jcs.265369","url":null,"abstract":"Entry of intracellular pathogenic bacteria is widely considered an actin-driven process, potentially overlooking contributions of the microtubule cytoskeleton. Interphase microtubules exist in distinct states, but whether this heterogeneity influences bacterial entry is unknown. Here, using the obligate intracellular bacterium Chlamydia pneumoniae, we show that microtubules not only contribute to bacterial entry but that their pre-existing state influences host-cell permissiveness. Cells enriched in acetylated microtubules are preferentially infected, whereas detyrosinated microtubules show no such association. Taxol enhances entry, whereas selective elevation of microtubule acetylation with Tubacin does not, indicating that acetylation itself is insufficient and pointing instead to a permissive state associated with stabilized, long-lived microtubules. We next show that this relationship is reciprocal: infection induces a dose-dependent increase in microtubule acetylation that requires bacterial viability and is not reproduced by uptake of Yersinia pseudotuberculosis Invasin-coated beads, indicating that internalization alone is insufficient to trigger this response. Finally, to examine how early chlamydial proteins can alter microtubule behaviour, we assessed the effect of the secreted TarP-family actin-microtubule regulator CPn0572 on microtubule stability. In the Schizosaccharomyces pombe in vivo test system, CPn0572 promotes microtubule persistence and can act together with another chlamydial microtubule modulator to generate a combined microtubule phenotype. Together, these findings identify the pre-existing microtubule state as a determinant of Chlamydia pneumoniae entry, show that infection subsequently remodels the microtubule cytoskeleton, and demonstrate that an early chlamydial protein can directly alter microtubule behaviour.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149127572","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0