Journal of cell science最新文献_第2页

Beyond cysts - organization of epithelial networks in the murine thymus. 囊外：小鼠胸腺上皮网络的组织。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-10-15 Epub Date: 2025-10-06 DOI: 10.1242/jcs.264079

Stepan Vodopyanov, Leslie Gunther-Cummins, Sophia DesMarais, Xheni Nishku, Joseph Churaman, Hillary Guzik, Rotem Alon, Vera DesMarais, Frank Macaluso, George S Karagiannis

{"title":"Beyond cysts - organization of epithelial networks in the murine thymus.","authors":"Stepan Vodopyanov, Leslie Gunther-Cummins, Sophia DesMarais, Xheni Nishku, Joseph Churaman, Hillary Guzik, Rotem Alon, Vera DesMarais, Frank Macaluso, George S Karagiannis","doi":"10.1242/jcs.264079","DOIUrl":"10.1242/jcs.264079","url":null,"abstract":"The thymus originates from the third pharyngeal pouch endoderm, which also gives rise to respiratory tract elements. Here, we examined intrathymic cystic structures, long considered remnants of organogenesis. Through sequential histology and ultrastructural imaging, we uncovered that these 'cysts' are in fact continuous and structured epithelial networks embedded within the thymic parenchyma. These networks follow a conserved 'head-neck-funnel-tentacle' architecture spanning the trabeculae, cortex, corticomedullary junction (CMJ) and medulla. The head, typically glandular and ciliated, connects to a funnel enriched in diverse epithelial cell types - goblet, tuft, club, ionocyte-like, microfold and ciliated cells - at the CMJ. Tentacle-like projections sometimes extend into the medulla, often surrounding perivascular spaces. Luminal contents vary, with thymocytes and macrophages most abundant caudally. We also identified solitary medullary thymic epithelial cells with large ciliated cytoplasmic lumens, distinct from these epithelial networks. Electron microscopy suggested a respiratory identity and thymic-specific adaptations for the lining cells. These findings challenge the notion of thymic cysts as inert debris, and instead reveal a coherent, mimetic system with possible roles in thymocyte selection, maturation and egress.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primary cilia shape postnatal astrocyte development through Sonic Hedgehog signaling. 初级纤毛通过Sonic Hedgehog信号影响出生后星形胶质细胞的发育。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-10-15 Epub Date: 2025-05-19 DOI: 10.1242/jcs.263965

Rachel Bear, Steven A Sloan, Tamara Caspary

{"title":"Primary cilia shape postnatal astrocyte development through Sonic Hedgehog signaling.","authors":"Rachel Bear, Steven A Sloan, Tamara Caspary","doi":"10.1242/jcs.263965","DOIUrl":"10.1242/jcs.263965","url":null,"abstract":"Primary cilia function as specialized signaling centers that regulate many cellular processes including neuron and glia development. Astrocytes possess cilia, but the function of cilia in astrocyte development remains largely unexplored. Crucially, dysfunction of either astrocytes or cilia contributes to the molecular changes observed in neurodevelopmental disorders. Here, we show that a subpopulation of developing astrocytes in the prefrontal cortex are ciliated. This population corresponds to proliferating astrocytes and largely expresses the ciliary protein ARL13B. Genetic ablation of astrocyte cilia in vivo at two distinct stages of astrocyte development results in changes to Sonic Hedgehog (Shh) transcriptional targets. We show that Shh activity is decreased in immature and mature astrocytes upon loss of cilia. Furthermore, loss of cilia in immature astrocytes results in decreased astrocyte proliferation and loss of cilia in mature astrocytes causes enlarged astrocyte morphology. Together, these results indicate that astrocytes require cilia for Shh signaling throughout development and uncover functions for astrocyte cilia in regulating astrocyte proliferation and maturation. This expands our fundamental knowledge of astrocyte development and cilia function to advance our understanding of neurodevelopmental disorders.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12148023/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143663530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel approach to tagging tubulin reveals microtubule assembly dynamics of the axoneme in Trypanosoma brucei. 一种标记微管蛋白的新方法揭示了布鲁氏锥虫轴突体的MT组装动力学。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-10-15 Epub Date: 2025-07-07 DOI: 10.1242/jcs.264145

Daniel Abbühl, Martina Pružincová, Luděk Štěpánek, Eleonore Bouscasse, Rita Azevedo, Mariette Matondo, Vladimir Varga, Serge Bonnefoy, Philippe Bastin

{"title":"A novel approach to tagging tubulin reveals microtubule assembly dynamics of the axoneme in Trypanosoma brucei.","authors":"Daniel Abbühl, Martina Pružincová, Luděk Štěpánek, Eleonore Bouscasse, Rita Azevedo, Mariette Matondo, Vladimir Varga, Serge Bonnefoy, Philippe Bastin","doi":"10.1242/jcs.264145","DOIUrl":"10.1242/jcs.264145","url":null,"abstract":"The protozoan parasite Trypanosoma brucei assembles a new flagellum while maintaining the existing one in the same cell. Our group has previously proposed a model where the mature flagellum is locked after construction to full length. To test this hypothesis directly, we monitored flagellum assembly dynamics through inducible expression of tubulin marked with an intragenic tag. We found that addition of new tubulin occurs at the distal flagellum tip at a linear rate and is indeed restricted to the new flagellum in bi-flagellated cells. Depleting the locking protein CEP164C prior to induction resulted in simultaneous integration of new tubulin in both flagella. This is direct evidence that trypanosomes avoid competition between the two flagella by allowing tubulin incorporation only in the new organelle. However, by tracing flagella over several cell cycles we also found that flagella do not remain locked forever. An orthogonal approach with HaloTag-tagged radial spoke protein 4/6 (GeneID Tb927.11.4480) supported these findings. Given that flagellum length in trypanosomes is stable, this indicates regular events of transient disassembly, followed by assembly, at the distal tip.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144173944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sub-ciliary localization of CEP290 and effects of its loss in mouse photoreceptors during development. CEP290的纤毛下定位及其在小鼠光感受器发育过程中丢失的影响。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-10-15 Epub Date: 2025-09-03 DOI: 10.1242/jcs.263869

Abigail R Moye, Michael A Robichaux, Melina A Agosto, Alexandre P Moulin, Alexandra Graff-Meyer, Carlo Rivolta, Theodore G Wensel

{"title":"Sub-ciliary localization of CEP290 and effects of its loss in mouse photoreceptors during development.","authors":"Abigail R Moye, Michael A Robichaux, Melina A Agosto, Alexandre P Moulin, Alexandra Graff-Meyer, Carlo Rivolta, Theodore G Wensel","doi":"10.1242/jcs.263869","DOIUrl":"10.1242/jcs.263869","url":null,"abstract":"The most common genetic cause of the childhood blindness disease Leber congenital amaurosis is mutation of the ciliopathy gene CEP290. Despite extensive study, the photoreceptor-specific roles of CEP290 remain unclear. Using advanced microscopy techniques, we investigated the sub-ciliary localization of CEP290 and its role in mouse photoreceptors during development. CEP290 was found throughout the connecting cilium between the microtubules and membrane, with nine-fold symmetry. In the absence of CEP290 ciliogenesis occurs, but the connecting cilium membrane is aberrant, and sub-structures, such as the ciliary necklace and Y-links, are confined to the proximal connecting cilium. Transition zone (TZ) proteins AHI1 and NPHP1 were abnormally restricted to the proximal connecting cilium in the absence of CEP290, whereas other TZ proteins, like NPHP8 and CEP89 were unaffected. Although outer segment disc formation is inhibited in Cep290 mutant retina, we observed large numbers of extracellular vesicles. These results suggest roles for CEP290 in ciliary membrane structure, outer segment disc formation and photoreceptor-specific spatial distribution of a subset of TZ proteins, which collectively lead to failure of outer segment formation and photoreceptor degeneration.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of locally activated spindle-associated proteins in oocytes uncovers a phosphatase-driven mechanism. 鉴定卵母细胞中局部激活的纺锤体相关蛋白揭示了磷酸酶驱动的机制。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-10-06 DOI: 10.1242/jcs.264161

Xiang Wan, Gera Pavlova, C Fiona Cullen, Igor Dasuzhau, Aleksandra Ciszek, Hiroyuki Ohkura

{"title":"Identification of locally activated spindle-associated proteins in oocytes uncovers a phosphatase-driven mechanism.","authors":"Xiang Wan, Gera Pavlova, C Fiona Cullen, Igor Dasuzhau, Aleksandra Ciszek, Hiroyuki Ohkura","doi":"10.1242/jcs.264161","DOIUrl":"https://doi.org/10.1242/jcs.264161","url":null,"abstract":"The meiotic spindle forms only around the chromosomes in oocytes, despite the exceptionally large volume of the cytoplasm. This spatial restriction is likely to be governed by local activation of key microtubule regulators around the chromosomes in oocytes, but the identities of these microtubule regulators and the mechanisms remain unclear. To address this, we developed a novel assay to visualise spatial regulation of spindle-associated proteins in Drosophila oocytes by inducing ectopic microtubule clusters. This assay identified several proteins including the TPX2 homologue Mei-38 that localise more strongly to microtubules near the chromosomes than away from them. In Mei-38, we identified a microtubule-binding domain containing a region highly conserved also in humans. The domain itself is regulated spatially, and contains a conserved serine and a nearby PP2A-B56 docking motif. A non-phosphorylatable mutation of this serine allows the domain to localise to ectopic microtubules as well as spindle microtubules, while mutations in a PP2A-B56 docking motif greatly reduced the spindle localisation. As this phosphatase is concentrated at the kinetochores, it may act as a novel chromosomal signal spatially regulating spindle proteins within oocytes.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145232664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lamin B loss in nuclear blebs is rupture dependent while increased DNA damage is rupture independent. 核泡中纤层蛋白B的损失与破裂有关，而DNA损伤的增加与破裂无关。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-10-06 DOI: 10.1242/jcs.263945

Catherine G Chu, Nick Lang, Erin Walsh, Mindy D Zheng, Gianna Manning, Kiruba Shalin, Lyssa M Cunha, Kate E Faucon, Nicholas Kam, Sara N Folan, Arav P Desai, Emily Naughton, Jaylynn Abreu, Alexis M Carson, Zachary L Wald, Dasha Khvorova-Wolfson, Leena Phan, Hannah Lee, Mai Pho, Kelsey Prince, Katherine Dorfman, Michael Seifu Bahiru, Andrew D Stephens

{"title":"Lamin B loss in nuclear blebs is rupture dependent while increased DNA damage is rupture independent.","authors":"Catherine G Chu, Nick Lang, Erin Walsh, Mindy D Zheng, Gianna Manning, Kiruba Shalin, Lyssa M Cunha, Kate E Faucon, Nicholas Kam, Sara N Folan, Arav P Desai, Emily Naughton, Jaylynn Abreu, Alexis M Carson, Zachary L Wald, Dasha Khvorova-Wolfson, Leena Phan, Hannah Lee, Mai Pho, Kelsey Prince, Katherine Dorfman, Michael Seifu Bahiru, Andrew D Stephens","doi":"10.1242/jcs.263945","DOIUrl":"https://doi.org/10.1242/jcs.263945","url":null,"abstract":"The nucleus must maintain shape and integrity to protect the function of the genome. Nuclear blebs are deformations identified by decreased DNA density that commonly lead to rupture. Lamin B levels often vary drastically between blebs. We tracked rupture via time lapse imaging of NLS-GFP into immunofluorescence of lamins and known rupture markers. We find that lamin B1 loss consistently marks ruptured nuclear blebs better than lamin A/C, emerin and cGAS. Visualizing post-rupture lamin B1 loss and emerin enrichment reveals that cell lines display widely different propensities for nuclear bleb rupture. To determine how rupture affects DNA damage, we time lapse imaged ruptured and unruptured blebs, then conducted immunofluorescence on the same cells for DNA damage markers γH2AX and 53BP1. We find that DNA damage is increased in blebbed nuclei independent of rupture. This was verified in blebbed LNCaP nuclei, which do not rupture and maintain lamin B1, but still show increased DNA damage. Thus, we confirm that lamin B is the most consistent marker of nuclear rupture, and that blebbed nuclei have increased DNA damage regardless of rupture.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145232621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Distinct impacts of human co-chaperone UNC45 paralogues on Drosophila muscle development and function. 人类共同伴侣UNC45对果蝇肌肉发育和功能的不同影响。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-10-02 DOI: 10.1242/jcs.263919

Daniel A Smith, Morgan I Mullens, Raul Ramos, Girish C Melkani, Sanford I Bernstein

{"title":"Distinct impacts of human co-chaperone UNC45 paralogues on Drosophila muscle development and function.","authors":"Daniel A Smith, Morgan I Mullens, Raul Ramos, Girish C Melkani, Sanford I Bernstein","doi":"10.1242/jcs.263919","DOIUrl":"https://doi.org/10.1242/jcs.263919","url":null,"abstract":"Uncoordinated-45 (UNC45) is a conserved protein required for myosin accumulation during muscle development. Invertebrates have one unc-45 gene whereas vertebrates have two paralogues, UNC45A and UNC45B, which exhibit differential expression patterns. We used the Drosophila model to investigate the ability of the vertebrate proteins to function in an invertebrate system, as well as the potential evolutionary redundancy of its human paralogues. Transgenic expression of either human UNC45 paralogue early in indirect flight muscle development resulted in impaired flight, disordered muscle organization, and unique sub-sarcomere localizations. We then generated chimeric proteins that replaced each of three Drosophila Unc-45 domains with their human cognates. We found that a chimera containing the myosin-binding UCS domain of human UNC45A impaired muscle function, while none of the UNC45B domain chimeras significantly impacted flight ability. Overall, our study shows that there is significant evolutionary divergence between vertebrate and invertebrate paralogues and that the human proteins differentially disrupt Drosophila myofibril assembly and function, suggesting that they are functionally unique.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145206375","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular models of the sperm head-tail coupling apparatus. 精子头尾耦合装置的分子模型。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-10-01 Epub Date: 2025-10-08 DOI: 10.1242/jcs.264168

Danielle B Buglak, Brian J Galletta, Nasser M Rusan

引用次数: 0

Importin α regulates ciliogenesis and cilia length with implications for Xenopus nephrogenesis. 输入蛋白α调节纤毛发生和纤毛长度与爪蟾肾形成的关系。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-09-29 DOI: 10.1242/jcs.264441

Natalie Mosqueda, Patrick James Sutton, Christopher W Brownlee

{"title":"Importin α regulates ciliogenesis and cilia length with implications for Xenopus nephrogenesis.","authors":"Natalie Mosqueda, Patrick James Sutton, Christopher W Brownlee","doi":"10.1242/jcs.264441","DOIUrl":"https://doi.org/10.1242/jcs.264441","url":null,"abstract":"Cilia are microtubule-based organelles essential for a wide range of biological processes ranging from facilitating fluid flow to transducing developmental and growth signals. Defects in cilia structure or function can lead to ciliopathies. Ciliogenesis and cilia length regulation depend on protein transport to the ciliary base, but the underlying molecular mechanisms remain unclear. Here we identify that the nuclear adapter protein, importin α, has conserved localization in human epithelial primary cilia and Xenopus laevis (X. laevis) epidermal multiciliated cells. Importin α regulates both ciliogenesis and cilia length maintenance, dependent on its localization to the membrane via palmitoylation or in the cytoplasm when not palmitoylated. In addition, we identify key ciliary proteins, CEP164 and ARL13B, as candidate binding partners of importin α through their NLS sequence and the requirement of this binding interaction for proper ciliogenesis and cilia length. Disruption of importin α palmitoylation in X. laevis causes defects in nephrogenesis which is rescued by forced membrane localization of importin α. These findings reveal a previously unrecognized role for importin α in cilia biology and advances understanding of congenital kidney diseases.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145185970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cellular elasticity drives the mechano-adaptation against fluid shear stress. 细胞弹性驱动对流体剪切应力的机械适应。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-09-26 DOI: 10.1242/jcs.264293

Ditipriya Mallick, Indranil Ghosh, Tanmoy Mondal, Sourav Mondal, Rupa Mukhopadhyay, Jomon Joseph, Somiranjan Ghosh, Siddhartha Sankar Jana

{"title":"Cellular elasticity drives the mechano-adaptation against fluid shear stress.","authors":"Ditipriya Mallick, Indranil Ghosh, Tanmoy Mondal, Sourav Mondal, Rupa Mukhopadhyay, Jomon Joseph, Somiranjan Ghosh, Siddhartha Sankar Jana","doi":"10.1242/jcs.264293","DOIUrl":"https://doi.org/10.1242/jcs.264293","url":null,"abstract":"Cancer cells adapt to external biophysical cues but how the cytoskeletal remodeling facilitate this mechano-adaptation is largely unexplored. Here, we demonstrate that the intrinsic non-muscle myosinII (NMII) activity and self-organization in cancer cells regulate the cellular elastic property when cells are exposed to fluid shear stress (FSS). In association with the reorganized actin filament network, NMII bipolar filaments can assemble into aligned stacks, which allow cellular stretching upon exposure to FSS. Inhibition of NMIIs by siRNA, (-) blebbistatin or Y27632 impairs the stack formation and perturbs cellular elasticity. Moreover, NMII-mediated elasticity regulates cyto-nuclear coupling through its association with LINC complex protein, Nesprin-2, and regulates nuclear import of the mechanoresponsive proteins, YAP/TAZ, which induce differential expression of genes thus decreasing growth and migration in FSS-exposed cells. These findings reveal that the cellular elasticity mediated by NMII dynamics provides mechano-adaptation against a mechanical stress, like FSS.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145149222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0