Journal of cell science最新文献_第4页

Resolution in super-resolution microscopy - facts, artifacts, technological advancements and biological applications. 超分辨率显微镜的分辨率-事实，人工制品，技术进步和生物学应用。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-05-15 Epub Date: 2025-05-27 DOI: 10.1242/jcs.263567

Kirti Prakash, David Baddeley, Christian Eggeling, Reto Fiolka, Rainer Heintzmann, Suliana Manley, Aleksandra Radenovic, Hari Shroff, Carlas Smith, Lothar Schermelleh

{"title":"Resolution in super-resolution microscopy - facts, artifacts, technological advancements and biological applications.","authors":"Kirti Prakash, David Baddeley, Christian Eggeling, Reto Fiolka, Rainer Heintzmann, Suliana Manley, Aleksandra Radenovic, Hari Shroff, Carlas Smith, Lothar Schermelleh","doi":"10.1242/jcs.263567","DOIUrl":"10.1242/jcs.263567","url":null,"abstract":"Super-resolution microscopy (SRM) has undeniable potential for scientific discovery, yet still presents many challenges that hinder its widespread adoption, including technical trade-offs between resolution, speed and photodamage, as well as limitations in imaging live samples and larger, more complex biological structures. Furthermore, SRM often requires specialized expertise and complex instrumentation, which can deter biologists from fully embracing the technology. In this Perspective, a follow-up to our recent Q&A article, we aim to demystify these challenges by addressing common questions and misconceptions surrounding SRM. Experts offer practical insights into how biologists can maximize the benefits of SRM while navigating issues such as photobleaching, image artifacts and the limitations of existing techniques. We also highlight recent developments in SRM that continue to push the boundaries of resolution. Our goal is to equip researchers with the crucial knowledge they need to harness the full potential of SRM.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":"138 10","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12148039/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144150416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The P4-phospholipid flippase Atp11a is required for maintenance of eye and ear structure in zebrafish. p4 -磷脂翻转酶Atp11a是维持斑马鱼眼睛和耳朵结构所必需的。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-05-15 Epub Date: 2025-05-22 DOI: 10.1242/jcs.263657

Alexia Hawkey-Noble, Cameron Tobin, Muhammad T Ameen, Liam Osmond, Colby Gill, Christina S Bottaro, Terry-Lynn Young, Curtis R French

{"title":"The P4-phospholipid flippase Atp11a is required for maintenance of eye and ear structure in zebrafish.","authors":"Alexia Hawkey-Noble, Cameron Tobin, Muhammad T Ameen, Liam Osmond, Colby Gill, Christina S Bottaro, Terry-Lynn Young, Curtis R French","doi":"10.1242/jcs.263657","DOIUrl":"10.1242/jcs.263657","url":null,"abstract":"The atp11a gene encodes a phospholipid flippase protein required to flip phosphatidylserine (PS) and phosphatidylethanolamine (PE) from the outer leaflet of the cytoplasmic membrane to the inner leaflet. Mutations in ATP11A have been described in individuals with sensorineural hearing loss and neurological deterioration; however, little is known regarding the mechanism by which loss of atp11a results in such phenotypes. To this end, we created loss-of-function atp11a mutant zebrafish to characterize potential disease states. We demonstrate that mutant atp11a zebrafish display a reduced number of stereocilia in the larval ear and a reduced number of hair cells in some sensory neuromasts, indicating that these fish represent an ideal model for studying atp11a-attributable hearing loss. In addition, atp11a mutant zebrafish raised in a standard light cycle have reduced photoreceptor outer segments, the severity of which is lessened when mutant larvae are raised in the dark. Photoreceptors that do remain in homozygous atp11a mutants undergo mitochondrial fission and produce an increased number of mitochondria, suggesting that defects in energy homeostasis may contribute to or result from outer segment degradation.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12148042/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Abnormal nucleoli architecture and aggregate formation in nucleophosmin mutated acute myeloid leukaemia. 核蛋白突变的急性髓性白血病核仁结构和聚集形成异常。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-05-15 Epub Date: 2025-05-21 DOI: 10.1242/jcs.263553

Martin Grundy, Kellie Lucken, Xiaomeng Xing, Eva L Simpson, Alice Worker, Ahmed Bayyoomi, Alison J Beckett, Ian A Prior, Daniel G Booth, Claire H Seedhouse

{"title":"Abnormal nucleoli architecture and aggregate formation in nucleophosmin mutated acute myeloid leukaemia.","authors":"Martin Grundy, Kellie Lucken, Xiaomeng Xing, Eva L Simpson, Alice Worker, Ahmed Bayyoomi, Alison J Beckett, Ian A Prior, Daniel G Booth, Claire H Seedhouse","doi":"10.1242/jcs.263553","DOIUrl":"10.1242/jcs.263553","url":null,"abstract":"Mutations in the nucleophosmin (NPM1) gene represent the most common genetic alteration in acute myeloid leukaemia (AML) and result in mis-localisation of the mutated protein from a predominantly nucleolar localisation to a predominantly cytoplasmic distribution. Here, we use high resolution imaging to demonstrate that NPM1 is crucial for maintaining normal nucleoli architecture and specifically the integrity of the enigmatic nucleoli rim, the least understood nucleolar compartment. We demonstrate that cell lines and primary cells with NPM1 mutations from individuals with AML have aberrant nucleoli architecture; intriguingly this abnormal nucleolar phenotype is reversible. Using a surrogate for rRNA synthesis, we show that the aberrant phenotype is associated with differences in nucleolar function; specifically, activity of RNA polymerase I is increased in NPM1 mutated cells. Perinucleolar chromatin organisation is also markedly different in NPM1 mutant cells. Finally, we report the novel finding that NPM1 mutated protein forms distinct aggregates and characterise these for the first time. This work reveals how nucleolar organisation contributes to the molecular mechanisms underpinning NPM1-driven AML, revealing novel therapeutic vulnerabilities.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12148033/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144003120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coilin and SUMOylation influence PARP1 dynamics and the DNA damage response. Coilin和SUMOylation影响PARP1动力学和DNA损伤反应。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-05-15 Epub Date: 2025-05-21 DOI: 10.1242/jcs.263953

Sara K Tucker, Blaise C Seale, David T Brown, Michael D Hebert

{"title":"Coilin and SUMOylation influence PARP1 dynamics and the DNA damage response.","authors":"Sara K Tucker, Blaise C Seale, David T Brown, Michael D Hebert","doi":"10.1242/jcs.263953","DOIUrl":"10.1242/jcs.263953","url":null,"abstract":"Coilin is a nucleoplasmic protein that is enriched in some cell types in the Cajal body (CB). CBs take part in the biogenesis of many different types of ribonucleoproteins (RNPs), such as small nuclear RNPs. Coilin is known as the CB marker protein and is required for CB formation. The function of nucleoplasmic coilin is less understood and has been shown to impact protein modification by SUMO, the small ubiquitin-like modifier. Additionally, it is known that coilin is recruited to sites of DNA damage caused by UVA exposure or expression of herpes simplex viral protein. PARP1, a DNA damage response protein, has been shown to be SUMOylated by PIAS4, a SUMO E3 ligase that associates with coilin. Here, we show that SUMOylation of PARP1 is lessened when coilin is suppressed. We also found that coilin knockdown and a SUMO inhibitor drug, TAK-981, influence the dynamics of PARP1 in response to micro-irradiation. Additionally, we find that the SUMOylation status of coilin influences its mobility in the CB and recruitment to sites of DNA damage. These data demonstrate that coilin and SUMOylation both have an influence on the DNA damage response.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12148040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality control of un-imported mitochondrial proteins at a glance. 非进口线粒体蛋白的质量控制一目了然。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-05-01 Epub Date: 2025-05-12 DOI: 10.1242/jcs.263757

Megan Balzarini, John Kim, Hilla Weidberg

引用次数: 0

Drosophila Clu ribonucleoprotein particle dynamics rely on the availability of functional Clu and translating ribosomes. 果蝇Clu核糖核蛋白颗粒动力学依赖于功能性Clu和翻译核糖体的可用性。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-05-01 Epub Date: 2025-05-09 DOI: 10.1242/jcs.263730

Hye Jin Hwang, Kelsey M Sheard, Rachel T Cox

{"title":"Drosophila Clu ribonucleoprotein particle dynamics rely on the availability of functional Clu and translating ribosomes.","authors":"Hye Jin Hwang, Kelsey M Sheard, Rachel T Cox","doi":"10.1242/jcs.263730","DOIUrl":"10.1242/jcs.263730","url":null,"abstract":"Drosophila Clu is a conserved multi-domain ribonucleoprotein essential for mitochondrial function that forms dynamic particles within the cytoplasm. Unlike stress granules and processing bodies (P-bodies), Clu particles disassemble under nutritional or oxidative stress. However, it is unclear how disrupting protein synthesis affects Clu particle dynamics, especially given that Clu binds mRNA and ribosomes. Here, we capitalize on ex vivo and in vivo imaging of Drosophila female germ cells to determine what domains of Clu are necessary for Clu particle assembly and how manipulating translation affects particle dynamics. Using domain deletion analysis, we identified three domains of Clu essential for particle assembly. We also demonstrated that overexpressing functional Clu led to disassembly of particles. In addition, we inhibited translation using cycloheximide and puromycin. In contrast to P-bodies, cycloheximide treatment did not disassemble Clu particles yet puromycin treatment did. Surprisingly, cycloheximide stabilized particles under oxidative and nutritional stress. These findings demonstrate that Clu particles display novel dynamics in response to altered ribosome activity and support a model where they function as translation hubs whose assembly heavily depends on the dynamic availability of translating ribosomes.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":"138 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12136168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Snapshots of mitochondrial fission imaged by cryo-scanning transmission electron tomography. 低温扫描透射电子断层成像的线粒体裂变快照。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-05-01 Epub Date: 2025-05-14 DOI: 10.1242/jcs.263639

Peter Kirchweger, Sharon Grayer Wolf, Neta Varsano, Tali Dadosh, Guenter P Resch, Michael Elbaum

{"title":"Snapshots of mitochondrial fission imaged by cryo-scanning transmission electron tomography.","authors":"Peter Kirchweger, Sharon Grayer Wolf, Neta Varsano, Tali Dadosh, Guenter P Resch, Michael Elbaum","doi":"10.1242/jcs.263639","DOIUrl":"https://doi.org/10.1242/jcs.263639","url":null,"abstract":"Mitochondria undergo constant remodeling via fission, fusion, extension and degradation. Fission, in particular, depends on the accumulation of mitochondrial fission factor (MFF) and subsequent recruitment of the dynamin-related protein DRP1 (also known as DNM1L). We used cryo-scanning transmission electron tomography (cryo-STET) to investigate mitochondrial morphologies in MFF mutant (MFF-/-) mouse embryonic fibroblast (MEF) cells in ATP-depleting conditions that normally induce fission. The capability of cryo-STET to image through the cytoplasmic volume to a depth of 1 µm facilitated visualization of intact mitochondria and their surroundings. We imaged changes in mitochondrial morphology and cristae structure, as well as contacts with the endoplasmic reticulum (ER), degradative organelles and the cytoskeleton at stalled fission sites. We found disruption of the outer mitochondrial membrane at contact sites with the ER and degradative organelles at sites of mitophagy. We identified fission sites where the inner mitochondrial membrane is already separated while the outer membrane is still continuous. Although MFF is a general fission factor, these observations demonstrate that mitochondrial fission can proceed to the final stage in its absence. The use of cryo-STET allays concerns about the loss of structures due to sample thinning required for tomography using cryo-transmission electron microscopy.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":"138 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondria-membranous organelle contacts at a glance. 线粒体-膜细胞器接触一目了然。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-05-01 Epub Date: 2025-05-13 DOI: 10.1242/jcs.263895

Antigoni Diokmetzidou, Luca Scorrano

引用次数: 0

Definition of the human mitochondrial TOM interactome reveals TRABD as a new interacting protein. 人类线粒体TOM相互作用组的定义表明TRABD是一种新的相互作用蛋白。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-05-01 Epub Date: 2025-04-24 DOI: 10.1242/jcs.263576

Metin Özdemir, Silke Oeljeklaus, Alexander Schendzielorz, Marcel Morgenstern, Anusha Valpadashi, Roya Yousefi, Bettina Warscheid, Sven Dennerlein

引用次数: 0

Spatial regulation of mitochondrial membrane potential by α5β1 integrin engagement in collective cell migration. α5β1整合素参与细胞集体迁移对线粒体膜电位的空间调控。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2025-05-01 Epub Date: 2025-05-12 DOI: 10.1242/jcs.263665

Gustavo G Pacheco, Bette J Dzamba, Wakako Endo, Benjamin C Edwards, Minah Khan, Tien Comlekoglu, David R Shook, Keri Quasey, Maureen A Bjerke, Glen D Hirsh, David F Kashatus, Douglas W DeSimone

{"title":"Spatial regulation of mitochondrial membrane potential by α5β1 integrin engagement in collective cell migration.","authors":"Gustavo G Pacheco, Bette J Dzamba, Wakako Endo, Benjamin C Edwards, Minah Khan, Tien Comlekoglu, David R Shook, Keri Quasey, Maureen A Bjerke, Glen D Hirsh, David F Kashatus, Douglas W DeSimone","doi":"10.1242/jcs.263665","DOIUrl":"10.1242/jcs.263665","url":null,"abstract":"The mechanistic links between mechanical forces and bioenergetics remain elusive. We report an increase in mitochondrial membrane potential (MMP) along the leading row of collectively migrating Xenopus laevis mesendoderm cells at sites where fibronectin-α5β1 integrin substrate traction stresses are greatest. Real-time metabolic analyses reveal α5β1 integrin-dependent increases in respiration efficiency in cells on fibronectin substrates. Elevation of metabolic activity is reduced following pharmacologic inhibition of focal adhesion kinase (FAK; also known as PTK2) signaling. Attachment of mesendoderm cells to fibronectin fragments that support differing α5β1 integrin conformational and ligand-binding affinity states, increases MMP when both the Arg-Gly-Asp (RGD) and Pro-Pro-Ser-Arg-Asn (PPSRN) synergy sites of fibronectin are engaged by the receptor. Cell stretch on deformable fibronectin substrates also results in a FAK-dependent increase in MMP. Inhibition of MMP or ATP-synthase activity slows collective cell migration velocity in vivo, further suggesting that integrin-dependent adhesion and signaling contribute to metabolic changes. These data highlight an underexplored link between extracellular matrix (ECM)-integrin adhesion and metabolic activity in embryonic cell migration. We propose that fibronectin-integrin adhesion and signaling help shape the metabolic landscape of collectively migrating cells.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":"138 9","pages":""},"PeriodicalIF":3.3,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12136171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0