Journal of cell science最新文献_第5页

The integrin odyssey - a journey full of fundamental discoveries. 整合的奥德赛-一个充满基本发现的旅程。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-09-15 Epub Date: 2025-09-24 DOI: 10.1242/jcs.263999

Reinhard Fässler, Arnoud Sonnenberg

引用次数: 0

Correction: Approaches to reduce succinate accumulation by restoration of succinate dehydrogenase activity in cultured adrenal cells. 纠正：通过恢复培养肾上腺细胞中琥珀酸脱氢酶活性来减少琥珀酸积累的方法。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-09-15 Epub Date: 2025-10-01 DOI: 10.1242/jcs.264405

引用次数: 0

Microtubules regulate tissue-level navigation in skin-resident macrophages. 微管调节皮肤巨噬细胞的组织水平导航。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-09-15 Epub Date: 2025-09-18 DOI: 10.1242/jcs.264101

Eric Peterman, Andrew Murphy, Ian A Swinburne, Tor H Linbo, Sean G Megason, Jeffrey P Rasmussen

{"title":"Microtubules regulate tissue-level navigation in skin-resident macrophages.","authors":"Eric Peterman, Andrew Murphy, Ian A Swinburne, Tor H Linbo, Sean G Megason, Jeffrey P Rasmussen","doi":"10.1242/jcs.264101","DOIUrl":"10.1242/jcs.264101","url":null,"abstract":"Immune cells rapidly respond to tissue damage through dynamic properties of the cytoskeleton. How microtubules control immune cell functions during injury responses remains poorly understood. Within skin, tissue-resident macrophages known as Langerhans cells use dynamic dendrites to surveil the epidermis for damage and migrate through a densely packed epithelium to wounds. Here, we used Langerhans cells within the adult zebrafish epidermis as a model to investigate roles of microtubules in immune cell tissue surveillance, phagocytosis and directed migration. We describe microtubule organization within Langerhans cells and show that depolymerizing the microtubule cytoskeleton alters dendrite morphology, debris engulfment and migration efficiency. We found that the microtubule organizing center positions adjacent to engulfed debris and that its position correlates with navigational pathfinding during tissue-level migration. Stabilizing microtubules inhibits Langerhans cell motility during directed cell migration by impairing navigation around cellular obstacles. Collectively, our work demonstrates requirements for microtubules in the dynamic actions of tissue-resident macrophages during epithelial surveillance and wound repair.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144873373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Supracellular contractility in Xenopus embryo epithelia regulated by extracellular ATP and the purinergic receptor P2Y2. 胞外ATP和嘌呤能受体P2Y2对爪蟾胚胎上皮细胞上收缩性的调控。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-09-15 Epub Date: 2025-10-02 DOI: 10.1242/jcs.263877

Sagar D Joshi, Timothy R Jackson, Lin Zhang, Carsten Stuckenholz, Lance A Davidson

{"title":"Supracellular contractility in Xenopus embryo epithelia regulated by extracellular ATP and the purinergic receptor P2Y2.","authors":"Sagar D Joshi, Timothy R Jackson, Lin Zhang, Carsten Stuckenholz, Lance A Davidson","doi":"10.1242/jcs.263877","DOIUrl":"10.1242/jcs.263877","url":null,"abstract":"Extracellular signals regulate epithelial homeostasis, cell fate and the patterning of cell behaviors during embryogenesis, wound healing, regeneration and disease progression. Previous studies in our group found that cell lysate from intentionally wounded Xenopus laevis embryos triggers a strong but transient contraction in neighboring epithelia, whether contiguous to the wound site or in non-wounded embryos. We previously identified extracellular ATP (eATP) as a possible candidate signal. Here we test additional candidates and find that several nucleotides, including ADP, UTP and UDP, also trigger contractility. Through a temporal and spatial screen of lysate activity, an inhibitor screen and morpholino knockdown of candidate receptors, we find that contractility is mediated by a G-protein-coupled purinergic receptor, P2Y2 (P2RY2). Activated P2RY2 triggers F-actin assembly and myosin II contractility. Knockdown of P2RY2 or overexpression of mutant G protein effectors abrogate epithelial contractility when epithelia are exposed to eATP or lysate. We demonstrate that the major contributors to epithelial contractility in lysate are the extracellular nucleotide triphosphates ATP and UTP, which are sensed by P2RY2 and transduced through G proteins to contract the epithelium.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immune cell interactions in development, homeostasis and immunity. 免疫细胞在发育、体内平衡和免疫中的相互作用。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-09-15 Epub Date: 2025-10-01 DOI: 10.1242/jcs.264374

Edward Jenkins, Clare Latta, Hoang Anh Le

引用次数: 0

Surface protein machineries in Gram-negative bacteria. 革兰氏阴性菌的表面蛋白机制。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-09-15 Epub Date: 2025-09-24 DOI: 10.1242/jcs.263624

Claire Overly Cottom, Evan Billings, Michelle Bush, Dorothy DRozario, Wyatt Scherschel, Nicholas Noinaj

{"title":"Surface protein machineries in Gram-negative bacteria.","authors":"Claire Overly Cottom, Evan Billings, Michelle Bush, Dorothy DRozario, Wyatt Scherschel, Nicholas Noinaj","doi":"10.1242/jcs.263624","DOIUrl":"https://doi.org/10.1242/jcs.263624","url":null,"abstract":"The major feature that distinguishes Gram-negative bacteria from Gram-positive bacteria is the presence of both an inner and outer membrane. Both membranes contain lipoproteins, peripheral membrane proteins, α-helical membrane proteins and a host of β-barrel membrane proteins. However, the vast majority of outer membrane proteins (OMPs) are β-barrel proteins, with a few select α-helical exceptions. In this Review, we provide a general overview of many of the proteins and protein machineries essential for cell survival that are commonly found on the surface of Gram-negative bacteria. Many of these proteins also serve important roles in mediating virulence in pathogenic strains, making them promising drug and vaccine targets. Examples include the β-barrel assembly machinery (BAM) complex, which is involved in the biogenesis and assembly of OMPs; surface lipoprotein assembly modulator 1 (Slam1), which is involved in the presentation of lipoproteins at the surface; efflux pumps, which remove potentially harmful substrates; as well as the protein assemblies that comprise pili and flagella. The structures of these protein machineries and the underlying mechanisms responsible for their function create a complex and dynamic surface landscape that must adapt quickly to ever-changing environmental conditions.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":"138 18","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145131021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impaired biogenesis of renin granules in juxtaglomerular cells of Vps33a (D251E) mutant mice. 小鼠Vps33a （D251E）突变体肾小球旁细胞肾素颗粒生物发生受损。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-09-15 Epub Date: 2025-09-25 DOI: 10.1242/jcs.263767

Huipeng Wang, Zhenhua Hao, Wei Li

引用次数: 0

An acentrosomal aster with atypical microtubule polarity recruits cytokinesis signals to its center in Xenopus egg extracts. 在非洲爪蟾卵提取物中，具有非典型微管极性的胞内体aster向其中心招募细胞分裂信号。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-09-15 Epub Date: 2025-09-26 DOI: 10.1242/jcs.263766

Ling Jin, Muchen Liu, Xianrui Cheng

引用次数: 0

Charcot-Marie-Tooth type 2A variants of mitofusin 2 sensitize cells to apoptotic cell death. Charcot-Marie-Tooth 2A型丝裂酶2变异体使细胞对凋亡细胞死亡敏感。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-09-15 Epub Date: 2025-09-25 DOI: 10.1242/jcs.263691

Mariana Joaquim, Maria-Bianca Bulimaga, Marie A Mohn, Solenn Plouzennec, Leon Osinski, Selver Altin, Esther Mahabir, Arnaud Chevrollier, Mafalda Escobar-Henriques

{"title":"Charcot-Marie-Tooth type 2A variants of mitofusin 2 sensitize cells to apoptotic cell death.","authors":"Mariana Joaquim, Maria-Bianca Bulimaga, Marie A Mohn, Solenn Plouzennec, Leon Osinski, Selver Altin, Esther Mahabir, Arnaud Chevrollier, Mafalda Escobar-Henriques","doi":"10.1242/jcs.263691","DOIUrl":"https://doi.org/10.1242/jcs.263691","url":null,"abstract":"The neuropathy Charcot-Marie-Tooth (CMT) is an incurable disease with a lack of genotype-phenotype correlation. Variants of the mitochondrial protein mitofusin 2 (MFN2), a large GTPase that mediates mitochondrial fusion, are responsible for the subtype CMT type 2A (CMT2A). Interestingly, beyond membrane remodelling, additional roles of MFN2 have been identified, expanding the possibilities to explore its involvement in disease. Here, we investigated how cellular functions of MFN2 are associated with variants present in individuals with CMT2A. Using human cellular models, we observed that cells expressing CMT2A variants display increased endoplasmic reticulum (ER) stress and apoptotic cell death. Increased cleavage of PARP1, caspase 9, caspase 7 and caspase 3, alongside BAX translocation to mitochondria, pointed towards effects on intrinsic apoptosis. Moreover, although disruption of fusion and fission dynamics per se did not correlate with cell death markers, expression of MFN1 or MFN2 alleviated the apoptosis markers of CMT2A variant cell lines. In sum, our results highlight excessive cell death by intrinsic apoptosis as a potential target in CMT2A disease.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":"138 18","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145137435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Object Stitching by Clustering of Adjacent Regions for accurate quantification of three-dimensional tissues. 为精确量化三维组织而设计的相邻区域聚类目标拼接。

IF 3.6 3区生物学

Journal of cell science Pub Date : 2025-09-15 Epub Date: 2025-09-26 DOI: 10.1242/jcs.264316

Mario Ledesma-Terrón, Diego Pérez-Dones, Diego Mazo-Durán, Gemma Navarro-Martinez, Gonzalo G Gíron, David G Míguez

{"title":"Object Stitching by Clustering of Adjacent Regions for accurate quantification of three-dimensional tissues.","authors":"Mario Ledesma-Terrón, Diego Pérez-Dones, Diego Mazo-Durán, Gemma Navarro-Martinez, Gonzalo G Gíron, David G Míguez","doi":"10.1242/jcs.264316","DOIUrl":"10.1242/jcs.264316","url":null,"abstract":"The study of tissue organization and morphogenesis requires quantitative analysis of 3D biological samples, a challenging task owing to limitations in imaging dense organs at single-cell resolution. Current 3D segmentation and quantification tools often struggle with the low resolution and signal-to-noise ratios typical of images taken in vivo or deep within tissues. To address this, we developed Object Stitching by Clustering of Adjacent Regions (OSCAR), a framework that combines machine learning with non-linear fitting and statistical algorithms specifically designed to quantify biological 3D stacks with high cellular density and low signal-to-background ratio based on nuclear staining. As proof of principle, the framework is applied to quantify the growth and organizational dynamics of the developing zebrafish vertebrate retina, showing that the cell numbers increase exponentially, while cell density increases and average nuclear volume decreases. Overall, its high accuracy, ease of use and reduced computational requirements establish OSCAR as a valuable tool for automated image analysis of densely packed tissues composed of cell subtypes that can be distinguished by specific labeling.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0