Journal of cell science最新文献_第5页

UVB radiation suppresses Dicer expression through β-catenin. 紫外线辐射通过β-catenin抑制Dicer的表达。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-15 Epub Date: 2024-11-26 DOI: 10.1242/jcs.261978

Zackie Aktary, Valérie Petit, Irina Berlin, Jeremy Raymond, Frederique Berger, Nisamanee Charoenchon, Evelyne Sage, Juliette Bertrand, Lionel Larue

{"title":"UVB radiation suppresses Dicer expression through β-catenin.","authors":"Zackie Aktary, Valérie Petit, Irina Berlin, Jeremy Raymond, Frederique Berger, Nisamanee Charoenchon, Evelyne Sage, Juliette Bertrand, Lionel Larue","doi":"10.1242/jcs.261978","DOIUrl":"10.1242/jcs.261978","url":null,"abstract":"Ultraviolet (UV) rays prompt a natural response in epidermal cells, particularly within melanocytes. The changes in gene expression and related signaling pathways in melanocytes following exposure to UV radiation are still not entirely understood. Our findings reveal that UVB irradiation suppresses the expression of Dicer (also known as Dicer1). This repression is intricately linked to the activation of the phosphoinositide 3-kinase (PI3K), ribosomal S6 kinase (RSK) and Wnt-β-catenin signaling pathways, and is directly associated with transcriptional repression by β-catenin (also known as CTNNB1). Notably, we have identified specific binding sites for the TCF/LEF-β-catenin complex in the Dicer promoter. Collectively, these results emphasize the significance of the UV-induced pathway involving the TCF/LEF-β-catenin complex, which impacts Dicer expression. UV radiation also reduced the levels of specific microRNAs known to be important in the biology of melanocytes. This pathway holds potential importance in governing melanocyte physiology.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11634033/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142501123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decreased DNA density is a better indicator of a nuclear bleb than lamin B loss. DNA 密度的降低比 Lamin B 的缺失更能说明核出血点的存在。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-06 DOI: 10.1242/jcs.262082

Samantha Bunner, Kelsey Prince, Emily M Pujadas Liwag, Nebiyat Eskndir, Karan Srikrishna, Antonia Amonu McCarthy, Anna Kuklinski, Olivia Jackson, Pedro Pellegrino, Shrushti Jagtap, Imuetiyan Eweka, Colman Lawlor, Emma Eastin, Griffin Yas, Julianna Aiello, Nathan LaPointe, Isabelle Schramm von Blucher, Jillian Hardy, Jason Chen, Schuyler Figueroa, Vadim Backman, Anne Janssen, Mary Packard, Katherine Dorfman, Luay Almassalha, Michael Seifu Bahiru, Andrew D Stephens

{"title":"Decreased DNA density is a better indicator of a nuclear bleb than lamin B loss.","authors":"Samantha Bunner, Kelsey Prince, Emily M Pujadas Liwag, Nebiyat Eskndir, Karan Srikrishna, Antonia Amonu McCarthy, Anna Kuklinski, Olivia Jackson, Pedro Pellegrino, Shrushti Jagtap, Imuetiyan Eweka, Colman Lawlor, Emma Eastin, Griffin Yas, Julianna Aiello, Nathan LaPointe, Isabelle Schramm von Blucher, Jillian Hardy, Jason Chen, Schuyler Figueroa, Vadim Backman, Anne Janssen, Mary Packard, Katherine Dorfman, Luay Almassalha, Michael Seifu Bahiru, Andrew D Stephens","doi":"10.1242/jcs.262082","DOIUrl":"10.1242/jcs.262082","url":null,"abstract":"Nuclear blebs are herniations of the nucleus that occur in diseased nuclei that cause nuclear rupture leading to cellular dysfunction. Chromatin and lamins are two of the major structural components of the nucleus that maintain its shape and function, but their relative roles in nuclear blebbing remain elusive. To determine the composition of nuclear blebs, we compared the immunofluorescence intensity of DNA and lamin B in the main nucleus body to the nuclear bleb across cell types and perturbations. DNA density in the nuclear bleb was consistently decreased to about half of the nuclear body while lamin B levels in the nuclear bleb varied widely. Partial Wave Spectroscopic (PWS) microscopy recapitulated significantly decreased likelihood of high-density domains in the nuclear bleb versus body, independent of lamin B. Time lapse imaging into immunofluorescence reveals that decreased DNA density marks all nuclear blebs while decreased lamin B1 levels only occur in blebs that have recently ruptured. Thus, decreased DNA density is a better marker of a nuclear bleb than lamin B level.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142583444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extravasation of immune and tumor cells from an endothelial perspective. 从内皮角度看免疫细胞和肿瘤细胞的外渗。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-01 Epub Date: 2024-11-12 DOI: 10.1242/jcs.262066

Amandine Dupas, Jacky G Goetz, Naël Osmani

{"title":"Extravasation of immune and tumor cells from an endothelial perspective.","authors":"Amandine Dupas, Jacky G Goetz, Naël Osmani","doi":"10.1242/jcs.262066","DOIUrl":"https://doi.org/10.1242/jcs.262066","url":null,"abstract":"Crossing the vascular endothelium is a necessary stage for circulating cells aiming to reach distant organs. Leukocyte passage through the endothelium, known as transmigration, is a multistep process during which immune cells adhere to the vascular wall, migrate and crawl along the endothelium until they reach their exit site. Similarly, circulating tumor cells (CTCs), which originate from the primary tumor or reseed from early metastatic sites, disseminate using the blood circulation and also must cross the endothelial barrier to set new colonies in distant organs. CTCs are thought to mimic arrest and extravasation utilized by leukocytes; however, their extravasation also requires processes that, from an endothelial perspective, are specific to cancer cells. Although leukocyte extravasation relies on maintaining endothelial impermeability, it appears that cancer cells can indoctrinate endothelial cells into promoting their extravasation independently of their normal functions. In this Review, we summarize the common and divergent mechanisms of endothelial responses during extravasation of leukocytes (in inflammation) and CTCs (in metastasis), and highlight how these might be leveraged in the development of anti-metastatic treatments.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":"137 21","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142620926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mature microRNA-binding protein QKI suppresses extracellular microRNA let-7b release. 成熟 microRNA 结合蛋白 QKI 可抑制细胞外 microRNA let-7b 的释放。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-01 Epub Date: 2024-11-06 DOI: 10.1242/jcs.261575

Kyung-Won Min, Kyoung-Min Choi, Hyejin Mun, Seungbeom Ko, Ji Won Lee, Cari A Sagum, Mark T Bedford, Young-Kook Kim, Joe R Delaney, Jung-Hyun Cho, Ted M Dawson, Valina L Dawson, Waleed Twal, Dong-Chan Kim, Clarisse H Panganiban, Hainan Lang, Xin Zhou, Seula Shin, Jian Hu, Tilman Heise, Sang-Ho Kwon, Dongsan Kim, Young Hwa Kim, Sung-Ung Kang, Kyungmin Kim, Sydney Lewis, Ahmet Eroglu, Seonghyun Ryu, Dongin Kim, Jeong Ho Chang, Junyang Jung, Je-Hyun Yoon

{"title":"Mature microRNA-binding protein QKI suppresses extracellular microRNA let-7b release.","authors":"Kyung-Won Min, Kyoung-Min Choi, Hyejin Mun, Seungbeom Ko, Ji Won Lee, Cari A Sagum, Mark T Bedford, Young-Kook Kim, Joe R Delaney, Jung-Hyun Cho, Ted M Dawson, Valina L Dawson, Waleed Twal, Dong-Chan Kim, Clarisse H Panganiban, Hainan Lang, Xin Zhou, Seula Shin, Jian Hu, Tilman Heise, Sang-Ho Kwon, Dongsan Kim, Young Hwa Kim, Sung-Ung Kang, Kyungmin Kim, Sydney Lewis, Ahmet Eroglu, Seonghyun Ryu, Dongin Kim, Jeong Ho Chang, Junyang Jung, Je-Hyun Yoon","doi":"10.1242/jcs.261575","DOIUrl":"10.1242/jcs.261575","url":null,"abstract":"Argonaute (AGO), a component of RNA-induced silencing complexes (RISCs), is a representative RNA-binding protein (RBP) known to bind with mature microRNAs (miRNAs) and is directly involved in post-transcriptional gene silencing. However, despite the biological significance of miRNAs, the roles of other miRNA-binding proteins (miRBPs) remain unclear in the regulation of miRNA loading, dissociation from RISCs and extracellular release. In this study, we performed protein arrays to profile miRBPs and identify 118 RBPs that directly bind to miRNAs. Among those proteins, the RBP quaking (QKI) inhibits extracellular release of the mature microRNA let-7b by controlling the loading of let-7b into extracellular vesicles via additional miRBPs such as AUF1 (also known as hnRNPD) and hnRNPK. The enhanced extracellular release of let-7b after QKI depletion activates Toll-like receptor 7 (TLR7) and promotes the production of proinflammatory cytokines in recipient cells, leading to brain inflammation in the mouse cortex. Thus, this study reveals the contribution of QKI to the inhibition of brain inflammation via regulation of extracellular let-7b release.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11574364/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142288103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bi-directional regulation between inflammation and stem cells in the respiratory tract. 呼吸道炎症和干细胞之间的双向调节。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-01 Epub Date: 2024-11-07 DOI: 10.1242/jcs.263413

Jinwook Choi, Jakub Chudziak, Joo-Hyeon Lee

{"title":"Bi-directional regulation between inflammation and stem cells in the respiratory tract.","authors":"Jinwook Choi, Jakub Chudziak, Joo-Hyeon Lee","doi":"10.1242/jcs.263413","DOIUrl":"10.1242/jcs.263413","url":null,"abstract":"Inflammation plays a crucial role in tissue injury, repair and disease, orchestrating a complex interplay of immune responses and cellular processes. Recent studies have uncovered the intricate connection between inflammation and stem cell dynamics, shedding light on the central role of stem cells in tissue regeneration. This Review highlights the significance of inflammation in shaping epithelial stem cell dynamics and its implications for tissue repair, regeneration and aging. We explore the multifaceted interactions between inflammation and stem cells, focusing on how inflammatory signals affect stem cell behavior and fate as well as the remodeling of their niche in the respiratory tract. We also discuss the concept of 'inflammatory memory' in epithelial stem cells, where prior inflammatory stimuli endow these cells with enhanced regenerative potential and confer long-lasting protective mechanisms for maintaining tissue integrity and function. Furthermore, we review the impact of cell senescence induced by inflammation on tissue regeneration and aging, delving into the molecular mechanisms underlying the modulation of signaling pathways, epigenetic modifications and cellular crosstalk. Understanding these dynamic processes not only deepens our knowledge of tissue homeostasis and repair but also holds profound implications for regenerative medicine strategies aimed at preventing pulmonary diseases.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":"137 21","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11574357/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604274","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A single-particle analysis method for detecting membrane remodelling and curvature sensing. 检测膜重塑和曲率感应的单颗粒分析方法。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-01 Epub Date: 2024-11-07 DOI: 10.1242/jcs.263533

Adeline Colussi, Leonardo Almeida-Souza, Harvey T McMahon

{"title":"A single-particle analysis method for detecting membrane remodelling and curvature sensing.","authors":"Adeline Colussi, Leonardo Almeida-Souza, Harvey T McMahon","doi":"10.1242/jcs.263533","DOIUrl":"10.1242/jcs.263533","url":null,"abstract":"In biology, shape and function are related. Therefore, it is important to understand how membrane shape is generated, stabilised and sensed by proteins and how this relates to organelle function. Here, we present an assay that can detect curvature preference and membrane remodelling with free-floating liposomes using protein concentrations in physiologically relevant ranges. The assay reproduced known curvature preferences of BAR domains and allowed the discovery of high-curvature preference for the PH domain of AKT and the FYVE domain of HRS (also known as HGS). In addition, our method reproduced the membrane vesiculation activity of the ENTH domain of epsin-1 (EPN1) and showed similar activity for the ANTH domains of PiCALM and Hip1R. Finally, we found that the curvature sensitivity of the N-BAR domain of endophilin inversely correlates to membrane charge and that deletion of its N-terminal amphipathic helix increased its curvature specificity. Thus, our method is a generally applicable qualitative method for assessing membrane curvature sensing and remodelling by proteins.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11574359/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142347441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

prominin-1-null Xenopus laevis develop subretinal drusenoid-like deposits, cone-rod dystrophy and RPE atrophy. Prominin-1缺失的爪蟾会出现视网膜下类风湿沉积、锥杆营养不良和 RPE 萎缩。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-01 Epub Date: 2024-11-12 DOI: 10.1242/jcs.262298

Brittany J Carr, Dominic Skitsko, Linnea M Kriese, Jun Song, Zixuan Li, Myeong Jin Ju, Orson L Moritz

{"title":"prominin-1-null Xenopus laevis develop subretinal drusenoid-like deposits, cone-rod dystrophy and RPE atrophy.","authors":"Brittany J Carr, Dominic Skitsko, Linnea M Kriese, Jun Song, Zixuan Li, Myeong Jin Ju, Orson L Moritz","doi":"10.1242/jcs.262298","DOIUrl":"10.1242/jcs.262298","url":null,"abstract":"Prominin-1 (PROM1) variants are associated with inherited, non-syndromic vision loss. We used CRISPR/Cas9 to induce prom1-null mutations in Xenopus laevis and then tracked retinal disease progression from the ages of 6 weeks to 3 years. We found that prom1-null-associated retinal degeneration in frogs was age-dependent and involved retinal pigment epithelium (RPE) dysfunction preceding photoreceptor degeneration. Before photoreceptor degeneration occurred, aging prom1-null frogs developed larger and increasing numbers of cellular debris deposits in the subretinal space and outer segment layer, which resembled subretinal drusenoid deposits (SDDs) in their location, histology and representation as seen by color fundus photography and optical coherence tomography (OCT). Evidence for an RPE origin of these deposits included infiltration of pigment granules into the deposits, thinning of the RPE as measured by OCT, and RPE disorganization as measured by histology and OCT. The appearance and accumulation of SDD-like deposits and RPE thinning and disorganization in our animal model suggests an underlying disease mechanism for prom1-null-mediated blindness that involves death and dysfunction of the RPE preceding photoreceptor degeneration, instead of direct effects upon photoreceptor outer segment morphogenesis, as was previously hypothesized.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142361605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A microtubule stability switch alters isolated vascular smooth muscle Ca2+ flux in response to matrix rigidity. 微管稳定性开关可改变离体血管平滑肌钙通量，以应对基质刚性。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-01 Epub Date: 2024-11-12 DOI: 10.1242/jcs.262310

Robert T Johnson, Finn Wostear, Reesha Solanki, Oliver Steward, Alice Bradford, Christopher Morris, Stefan Bidula, Derek T Warren

{"title":"A microtubule stability switch alters isolated vascular smooth muscle Ca2+ flux in response to matrix rigidity.","authors":"Robert T Johnson, Finn Wostear, Reesha Solanki, Oliver Steward, Alice Bradford, Christopher Morris, Stefan Bidula, Derek T Warren","doi":"10.1242/jcs.262310","DOIUrl":"10.1242/jcs.262310","url":null,"abstract":"During ageing, the extracellular matrix of the aortic wall becomes more rigid. In response, vascular smooth muscle cells (VSMCs) generate enhanced contractile forces. Our previous findings demonstrate that VSMC volume is enhanced in response to increased matrix rigidity, but our understanding of the mechanisms regulating this process remain incomplete. In this study, we show that microtubule stability in VSMCs is reduced in response to enhanced matrix rigidity via Piezo1-mediated Ca2+ influx. Moreover, VSMC volume and Ca2+ flux is regulated by microtubule dynamics; microtubule-stabilising agents reduced both VSMC volume and Ca2+ flux on rigid hydrogels, whereas microtubule-destabilising agents increased VSMC volume and Ca2+ flux on pliable hydrogels. Finally, we show that disruption of the microtubule deacetylase HDAC6 uncoupled these processes and increased α-tubulin acetylation on K40, VSMC volume and Ca2+ flux on pliable hydrogels, but did not alter VSMC microtubule stability. These findings uncover a microtubule stability switch that controls VSMC volume by regulating Ca2+ flux. Taken together, these data demonstrate that manipulation of microtubule stability can modify VSMC response to matrix stiffness.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11586521/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142288099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An improved tetracycline-inducible expression system for fission yeast. 改进的裂殖酵母四环素诱导表达系统。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-01 DOI: 10.1242/jcs.263404

Xiao-Hui Lyu, Yu-Sheng Yang, Zhao-Qian Pan, Shao-Kai Ning, Fang Suo, Li-Lin Du

{"title":"An improved tetracycline-inducible expression system for fission yeast.","authors":"Xiao-Hui Lyu, Yu-Sheng Yang, Zhao-Qian Pan, Shao-Kai Ning, Fang Suo, Li-Lin Du","doi":"10.1242/jcs.263404","DOIUrl":"10.1242/jcs.263404","url":null,"abstract":"The ability to manipulate gene expression is valuable for elucidating gene function. In the fission yeast Schizosaccharomyces pombe, the most widely used regulatable expression system is the nmt1 promoter and its two attenuated variants. However, these promoters have limitations, including a long lag, incompatibility with rich media and unsuitability for non-dividing cells. Here, we present a tetracycline-inducible system free of these shortcomings. Our system features the enotetS promoter, which achieves a similar induced level and a higher induction ratio compared to the nmt1 promoter, without exhibiting a lag. Additionally, our system includes four weakened enotetS variants, offering an expression range similar to that of the nmt1 series promoters but with more intermediate levels. To enhance usability, each promoter is combined with a Tet-repressor-expressing cassette in an integration plasmid. Importantly, our system can be used in non-dividing cells, enabling the development of a synchronous meiosis induction method with high spore viability. Moreover, our system allows for the shutdown of gene expression and the generation of conditional loss-of-function mutants. This system provides a versatile and powerful tool for manipulating gene expression in fission yeast.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142347442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Disordered hinge regions of the AP-3 adaptor complex promote vesicle budding from the late Golgi in yeast. AP-3适配体复合物的无序铰链区可促进酵母中囊泡从晚期高尔基体出芽。

IF 3.3 3区生物学

Journal of cell science Pub Date : 2024-11-01 Epub Date: 2024-11-08 DOI: 10.1242/jcs.262234

Mitchell Leih, Rachael L Plemel, Matt West, Cortney G Angers, Alexey J Merz, Greg Odorizzi

{"title":"Disordered hinge regions of the AP-3 adaptor complex promote vesicle budding from the late Golgi in yeast.","authors":"Mitchell Leih, Rachael L Plemel, Matt West, Cortney G Angers, Alexey J Merz, Greg Odorizzi","doi":"10.1242/jcs.262234","DOIUrl":"10.1242/jcs.262234","url":null,"abstract":"Vesicles bud from maturing Golgi cisternae in a programmed sequence. Budding is mediated by adaptors that recruit cargoes and facilitate vesicle biogenesis. In Saccharomyces cerevisiae, the AP-3 adaptor complex directs cargoes from the Golgi to the lysosomal vacuole. The AP-3 core consists of small and medium subunits complexed with two non-identical large subunits, β3 (Apl6) and δ (Apl5). The C-termini of β3 and δ were thought to be flexible hinges linking the core to ear domains that bind accessory proteins involved in vesicular transport. We found by computational modeling that the yeast β3 and δ hinges are intrinsically disordered and lack folded ear domains. When either hinge is truncated, AP-3 is recruited to the Golgi, but vesicle budding is impaired and cargoes normally sorted into the AP-3 pathway are mistargeted. This budding deficiency causes AP-3 to accumulate on ring-like Golgi structures adjacent to GGA adaptors that, in wild-type cells, bud vesicles downstream of AP-3 during Golgi maturation. Thus, each of the disordered hinges of yeast AP-3 has a crucial role in mediating transport vesicle formation at the Golgi.","PeriodicalId":15227,"journal":{"name":"Journal of cell science","volume":" ","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11574352/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142347444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0