Hypothesis that ancestral eukaryotes sexually proliferated without kinetochores or mitosis.

Abstract

Eukaryotes possess two different mechanisms to transmit genetic material - mitosis and meiosis. Because mitosis is universal in all present-day eukaryotes, it has been widely assumed, despite the absence of definitive evidence, that meiosis evolved from mitosis during eukaryogenesis. In both processes, chromosome movement depends on interactions between spindle microtubules and a macromolecular protein complex called the kinetochore that assembles onto centromere DNA. Spindle microtubules consist of α- and β-tubulin subunits, which are conserved in all studied eukaryotes. Similarly, canonical kinetochore components are found in almost all eukaryotes. However, an evolutionarily divergent group of organisms called kinetoplastids has a unique set of kinetochore proteins. It remains unclear why and when different types of kinetochores evolved. In this Hypothesis article, I propose that the last eukaryotic common ancestor (LECA) did not have a kinetochore and that these two kinetochore systems evolved independently - one in the ancestor of kinetoplastids and another in the ancestor of all other eukaryotes. Based on the notion that archaea and the LECA possessed cell fusion and genetic exchange machineries, I further propose that key aspects of meiosis evolved prior to mitosis, challenging the dogma that meiosis evolved from mitosis.