Journal of Cell Biology最新文献_第7页

FGFR2 residence in primary cilia is necessary for epithelial cell signaling. FGFR2在初级纤毛中的驻留是上皮细胞信号传导所必需的。

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2025-04-21 DOI: 10.1083/jcb.202311030

Alexandru Nita,Sara P Abraham,Eman R Elrefaay,Bohumil Fafilek,Eliska Cizkova,Vlad Constantin Ursachi,Iva Gudernova,Adolf Koudelka,Pooja Dudeja,Tomas Gregor,Zuzana Feketova,Gustavo Rico,Katerina Svozilova,Canan Celiker,Aleksandra A Czyrek,Tomas Barta,Lukas Trantirek,Antoni Wiedlocha,Pavel Krejci,Michaela Bosakova

{"title":"FGFR2 residence in primary cilia is necessary for epithelial cell signaling.","authors":"Alexandru Nita,Sara P Abraham,Eman R Elrefaay,Bohumil Fafilek,Eliska Cizkova,Vlad Constantin Ursachi,Iva Gudernova,Adolf Koudelka,Pooja Dudeja,Tomas Gregor,Zuzana Feketova,Gustavo Rico,Katerina Svozilova,Canan Celiker,Aleksandra A Czyrek,Tomas Barta,Lukas Trantirek,Antoni Wiedlocha,Pavel Krejci,Michaela Bosakova","doi":"10.1083/jcb.202311030","DOIUrl":"https://doi.org/10.1083/jcb.202311030","url":null,"abstract":"Primary cilium projects from cells to provide a communication platform with neighboring cells and the surrounding environment. This is ensured by the selective entry of membrane receptors and signaling molecules, producing fine-tuned and effective responses to the extracellular cues. In this study, we focused on one family of signaling molecules, the fibroblast growth factor receptors (FGFRs), their residence within cilia, and its role in FGFR signaling. We show that FGFR1 and FGFR2, but not FGFR3 and FGFR4, localize to primary cilia of the developing mouse tissues and in vitro cells. For FGFR2, we demonstrate that the ciliary residence is necessary for its signaling and expression of target morphogenic genes. We also show that the pathogenic FGFR2 variants have minimal cilium presence, which can be rescued for the p.P253R variant associated with the Apert syndrome by using the RLY-4008 kinase inhibitor. Finally, we determine the molecular regulators of FGFR2 trafficking to cilia, including IFT144, BBS1, and the conserved T429V430 motif within FGFR2.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"43 1","pages":""},"PeriodicalIF":7.8,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143857292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microtubule-driven cell shape changes and actomyosin flow synergize to position the centrosome. 微管驱动的细胞形状改变和肌动球蛋白流动协同作用来定位中心体。

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2025-04-17 DOI: 10.1083/jcb.202405126

Alexandre Schaeffer,Simona Buracco,Morgan Gazzola,Matthieu Gelin,Benoit Vianay,Chiara de Pascalis,Laurent Blanchoin,Manuel Théry

引用次数: 0

ZBTB17/MIZ1 promotes peroxisome biogenesis by transcriptional regulation of PEX13. ZBTB17/MIZ1通过转录调控PEX13促进过氧化物酶体的生物发生。

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2025-04-17 DOI: 10.1083/jcb.202407198

Hongqin Liu,Xi Chen,Hanlin Wang,Guanglei Zhuang,Zheng-Jiang Zhu,Min Zhuang

{"title":"ZBTB17/MIZ1 promotes peroxisome biogenesis by transcriptional regulation of PEX13.","authors":"Hongqin Liu,Xi Chen,Hanlin Wang,Guanglei Zhuang,Zheng-Jiang Zhu,Min Zhuang","doi":"10.1083/jcb.202407198","DOIUrl":"https://doi.org/10.1083/jcb.202407198","url":null,"abstract":"Peroxisomes are integral metabolic organelles involved in both catabolic and anabolic processes in humans, with defects linked to diseases. The functions of peroxisomes are regulated at transcriptional, translational, and posttranslational levels. In this study, we employed the CRISPR/Cas9-based screening of a ubiquitin ligase library to identify regulators of human peroxisomes. We discovered that ZBTB17 (MIZ1) plays a role in regulating the import of proteins into peroxisomes. Independent of its ubiquitin ligase activity, ZBTB17/MIZ1 operates as a transcription factor to modulate the expression of key importer PEX13, influencing the localization of peroxisomal enzymes. Furthermore, metabolomic profiling reveals that knockdown of ZBTB17 or PEX13 results in similar metabolic alterations, with downregulated purine synthesis. Collectively, we identify ZBTB17 as a key regulator of peroxisomal protein import, thereby affecting peroxisomal function and nucleotide metabolism. Our findings provide insights into the multifaceted regulation of peroxisomes in complex human cells and shed light on the molecular mechanisms underlying ZBTB17's role as a transcriptional regulator.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"3 1","pages":""},"PeriodicalIF":7.8,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PML mutants from arsenic-resistant patients reveal SUMO1-TOPORS and SUMO2/3-RNF4 degradation pathways. 来自砷抗性患者的PML突变体揭示了SUMO1-TOPORS和SUMO2/3-RNF4降解途径。

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2025-04-16 DOI: 10.1083/jcb.202407133

Ellis G Jaffray,Michael H Tatham,Barbara Mojsa,Anna Plechanovová,Alejandro Rojas-Fernandez,Julio C Y Liu,Niels Mailand,Adel F M Ibrahim,Graeme Ball,Iain M Porter,Ronald T Hay

{"title":"PML mutants from arsenic-resistant patients reveal SUMO1-TOPORS and SUMO2/3-RNF4 degradation pathways.","authors":"Ellis G Jaffray,Michael H Tatham,Barbara Mojsa,Anna Plechanovová,Alejandro Rojas-Fernandez,Julio C Y Liu,Niels Mailand,Adel F M Ibrahim,Graeme Ball,Iain M Porter,Ronald T Hay","doi":"10.1083/jcb.202407133","DOIUrl":"https://doi.org/10.1083/jcb.202407133","url":null,"abstract":"Arsenic effectively treats acute promyelocytic leukemia by inducing SUMO and ubiquitin-dependent degradation of the promyelocytic leukemia (PML)-retinoic acid receptor alpha oncogenic fusion protein. However, some patients relapse with arsenic-resistant disease because of missense mutations in PML. To determine the mechanistic basis for arsenic resistance, PML-/- cells were reconstituted with YFP fusions of wild-type PML-V and two common patient mutants: A216T and L217F. Both mutants were resistant to degradation by arsenic but for different biochemical reasons. Arsenic did not trigger SUMOylation of A216T PML, which failed to recruit the SUMO-targeting ubiquitin ligases RNF4 and TOPORS. L217F PML did respond with increased SUMO2/3 conjugation that facilitated RNF4 engagement but failed to reach the threshold of SUMO1 conjugation required to recruit TOPORS. Thus, neither mutant accumulated the appropriate polyubiquitin signal required for p97 binding. These PML mutants have revealed a convergence of SUMO1, SUMO2/3, TOPORS, and RNF4 that facilitates the arsenic-induced degradation of PML.","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"65 1","pages":""},"PeriodicalIF":7.8,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143846433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mitochondrial mayhem: Disrupting conserved N-terminal motifs in TANGO2 impacts its localization and function. 线粒体混乱：破坏 TANGO2 的保守 N 端基团会影响其定位和功能。

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2025-04-15 DOI: 10.1083/jcb.202503010

Sarah E Sandkuhler,Samuel J Mackenzie

引用次数: 0

Rab GTPases as "eat me" signals for selective autophagy. Rab GTPases是选择性自噬的 "吃我 "信号。

IF 7.8 1区生物学

Journal of Cell Biology Pub Date : 2025-04-10 DOI: 10.1083/jcb.202502030

Yan G Zhao

引用次数: 0

Subversion of the host endocytic pathway by Legionella pneumophila-mediated ubiquitination of Rab5. 嗜肺军团菌介导的Rab5泛素化对宿主内吞途径的颠覆。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2025-04-07 Epub Date: 2025-03-04 DOI: 10.1083/jcb.202406159

Shino Tanaka, Hiromu Oide, Shumma Ikeda, Mitsuo Tagaya, Hiroki Nagai, Tomoko Kubori, Kohei Arasaki

引用次数: 0

Translation of unspliced retroviral genomic RNA in the host cell is regulated in both space and time. 宿主细胞中未剪接的逆转录病毒基因组 RNA 的翻译在空间和时间上都受到调控。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2025-04-07 Epub Date: 2025-01-27 DOI: 10.1083/jcb.202405075

Felipe Leon-Diaz, Célia Chamontin, Sébastien Lainé, Marius Socol, Edouard Bertrand, Marylène Mougel

引用次数: 0

Any1 is a phospholipid scramblase involved in endosome biogenesis. Any1 是一种磷脂扰乱酶，参与内质体的生物生成。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2025-04-07 Epub Date: 2025-03-06 DOI: 10.1083/jcb.202410013

Jieqiong Gao, Rico Franzkoch, Cristian Rocha-Roa, Olympia Ekaterini Psathaki, Michael Hensel, Stefano Vanni, Christian Ungermann

引用次数: 0

Cytoplasmic ribosomes on mitochondria alter the local membrane environment for protein import. 线粒体上的细胞质核糖体改变了蛋白质输入的局部膜环境。

IF 7.4 1区生物学

Journal of Cell Biology Pub Date : 2025-04-07 Epub Date: 2025-03-06 DOI: 10.1083/jcb.202407110

Ya-Ting Chang, Benjamin A Barad, Juliette Hamid, Hamidreza Rahmani, Brian M Zid, Danielle A Grotjahn

{"title":"Cytoplasmic ribosomes on mitochondria alter the local membrane environment for protein import.","authors":"Ya-Ting Chang, Benjamin A Barad, Juliette Hamid, Hamidreza Rahmani, Brian M Zid, Danielle A Grotjahn","doi":"10.1083/jcb.202407110","DOIUrl":"10.1083/jcb.202407110","url":null,"abstract":"<p><p>Most of the mitochondria proteome is nuclear-encoded, synthesized by cytoplasmic ribosomes, and targeted to the mitochondria posttranslationally. However, a subset of mitochondrial-targeted proteins is imported co-translationally, although the molecular mechanisms governing this process remain unclear. We employ cellular cryo-electron tomography to visualize interactions between cytoplasmic ribosomes and mitochondria in Saccharomyces cerevisiae. We use surface morphometrics tools to identify a subset of ribosomes optimally oriented on mitochondrial membranes for protein import. This allows us to establish the first subtomogram average structure of a cytoplasmic ribosome at the mitochondrial surface in the native cellular context, which showed three distinct connections with the outer mitochondrial membrane surrounding the peptide exit tunnel. Further, this analysis demonstrated that cytoplasmic ribosomes primed for mitochondrial protein import cluster on the outer mitochondrial membrane at sites of local constrictions of the outer and inner mitochondrial membranes. Overall, our study reveals the architecture and the spatial organization of cytoplasmic ribosomes at the mitochondrial surface, providing a native cellular context to define the mechanisms that mediate efficient mitochondrial co-translational protein import.</p>","PeriodicalId":15211,"journal":{"name":"Journal of Cell Biology","volume":"224 4","pages":""},"PeriodicalIF":7.4,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11893167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0