Journal of Artificial Organs最新文献_第4页

Artificial pancreas: the past and the future. 人工胰腺：过去与未来。

IF 1.1 4区医学

Journal of Artificial Organs Pub Date : 2025-05-25 DOI: 10.1007/s10047-025-01510-1

Hiroyuki Kitagawa, Masaya Munekage, Satoru Seo, Kazuhiro Hanazaki

引用次数: 0

Effect of ECM nanostructures in decellularized small intestine on differentiation of intestinal epithelial model cells. 脱细胞小肠ECM纳米结构对肠上皮模型细胞分化的影响。

IF 1.1 4区医学

Journal of Artificial Organs Pub Date : 2025-05-22 DOI: 10.1007/s10047-025-01509-8

Towa Fukada, Sin Lam U, Naoko Nakamura

{"title":"Effect of ECM nanostructures in decellularized small intestine on differentiation of intestinal epithelial model cells.","authors":"Towa Fukada, Sin Lam U, Naoko Nakamura","doi":"10.1007/s10047-025-01509-8","DOIUrl":"https://doi.org/10.1007/s10047-025-01509-8","url":null,"abstract":"Caco-2 cells are derived from human colon cancer and have the ability to differentiate into human intestinal epithelial-like cells. The 2D in vitro intestinal model of Caco-2 cells cultured on a semi-permeable membrane is widely used in drug development and the evaluation of absorption functions. However, these intestinal models lack the structural characteristics of the small intestine in vivo, and the cell behavior is not properly controlled. Previous studies have reported that the microstructure of the villi and crypts on a small intestine-mimicking scaffold promotes Caco-2 differentiation; however, the effect of the nanostructure of the small intestine-mimicking scaffold on Caco-2 differentiation remains unclear. This study aimed to elucidate the effects of nanostructures on the small intestine mimetic scaffold in Caco-2 differentiation. We fabricated a decellularized small intestine in which the basement membrane nanostructure was altered through a subtractive process. Caco-2 cells were cultured on decellularized small intestine for 21 days, and the differentiation of Caco-2 cells was assessed. The microvillus density of Caco-2 cultured on decellularized small intestine that retained the unique nanostructure of small intestinal basement membrane was significantly higher than that of Caco-2 cultured on decellularized small intestine that did not retain the unique nanostructure of small intestinal basement membrane. This indicates that nanostructures specific to the basement membrane of the small intestine enhanced Caco-2 cell maturation.","PeriodicalId":15177,"journal":{"name":"Journal of Artificial Organs","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144119758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survival and hospitalization in home versus Institutional hemodialysis-nine years of follow up. 生存和住院在家与机构血液透析- 9年随访。

IF 1.1 4区医学

Journal of Artificial Organs Pub Date : 2025-05-18 DOI: 10.1007/s10047-025-01511-0

Verica Todorov Sakic, Petar Djuric, Ana Bulatovic, Jelena Bjedov, Aleksandar Jankovic, Snezana Pesic, Zivka Djuric, Radomir Naumovic

{"title":"Survival and hospitalization in home versus Institutional hemodialysis-nine years of follow up.","authors":"Verica Todorov Sakic, Petar Djuric, Ana Bulatovic, Jelena Bjedov, Aleksandar Jankovic, Snezana Pesic, Zivka Djuric, Radomir Naumovic","doi":"10.1007/s10047-025-01511-0","DOIUrl":"https://doi.org/10.1007/s10047-025-01511-0","url":null,"abstract":"Increasing interest in home dialysis treatments are driven by better outcomes, less complications, patients desire and economic reasons. We compared 26 prevalent home hemodialysis (HHD) patients with 52 matched institutional hemodialysis patients (IHD) in survival and morbidity. Median age for HHD and IHD patients was 55,7 and 56 years respectively, and 77% were men. HHD patients had significantly better anemia control (Hgb level 12.2 ± 1.7 vs. 10.8 ± 1.3gr/dl; p = 0,001 respectively), and significantly higher albumin and cholesterol levels than IHD (42.5 ± 2 vs. 39 ± 3 g/l, p = 0.001; 5.1 ± 1 vs.4.7 ± 0.8 mmol/l, p = 0.05, respectively). During the nine years of follow up, there was no difference between groups in overall number of hospitalization (3.7 ± 3.3 vs. 3.9 ± 2.8; p = 0.47), nor in annual admission rate for everyone cause (0.5 ± 0.4 vs. 0.6 ± 0.4, p = 0.28), but IHD patients stayed longer in hospital (7.4 ± 9.8 days vs. 9.3 ± 8.7 days; p = 0.05). Cause-specific morbidity showed that IHD patients had more frequent annual admission rate for cardiovascular diseases (CVD) than HHD (0.4 ± 0.3 vs. 0.2 ± 0.1 respectively, p = 0.05), while there were no differences for infections (0.3 ± 0.3 vs. 0.3 ± 0.2 respectively, p = 0.9) nor vascular access (VA) dysfunction (0.4 ± 0.3 vs. 0.3 ± 0.3 respectively, p = 0.3). Also annual in-hospital stay for CVD (3.0 ± 3.1 vs. 4.0 ± 4.5 days; p = 0.5), infection (6,4 ± 7,5 vs. 5,7 ± 7,6 days; p = 0,6) and VA dysfunction (6.0 ± 7.0 vs. 7.7 ± 7.8 days; p = 0,5) did not differ between HHD and IHD group. As revealed by Kaplan Meier curve, survival in HHD and IHD patients were 92.3% vs. 90.4% at 3 years, 84.6% vs. 70.2% at 5 years, and 55.7% vs. 50% at 9 years (log-rank test p = 0,5). HHD provides better anemia and nutrition control; shorter hospitalizations and less frequent hospitalizations for CVD.","PeriodicalId":15177,"journal":{"name":"Journal of Artificial Organs","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144093620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel blood volume monitoring approach in a venous reservoir using a laser displacement sensor for blood surface distance measurement. 一种利用激光位移传感器测量血液表面距离的静脉血容量监测新方法。

IF 1.1 4区医学

Journal of Artificial Organs Pub Date : 2025-05-16 DOI: 10.1007/s10047-025-01505-y

Shinichi Fukuhara, Ken-Ichiro Yamamoto

{"title":"A novel blood volume monitoring approach in a venous reservoir using a laser displacement sensor for blood surface distance measurement.","authors":"Shinichi Fukuhara, Ken-Ichiro Yamamoto","doi":"10.1007/s10047-025-01505-y","DOIUrl":"https://doi.org/10.1007/s10047-025-01505-y","url":null,"abstract":"A venous reservoir (VR) is crucial for safe blood volume regulation during cardiopulmonary bypass (CPB) procedures. Currently, blood volume measurement in VRs relies heavily on visual inspection, which limits its accuracy and responsiveness. In this paper, a method that leverages a laser displacement sensor for blood surface distance change detection in VRs with high accuracy and simplicity is proposed. It enables precise, non-contact, real-time measurements for displacement and position analysis of blood surface. Hard-shell VRs maintain a constant relationship between blood volume and blood surface height. Therefore, we measured the blood surface distance and the distance between the sensors. The findings of this study demonstrate that blood volume correlates with the distance measured from the blood surface to the sensor, showing clear \"blood surface distance-volume characteristic\" curves specific to each VR. Experimental results confirm excellent reproducibility and low variability, even under different conditions, such as blood dilution and varying ambient light. In addition, the blood volume dynamic behavior during CPB can be captured with measurements that are in good agreement with theoretical predictions. Our findings confirm the potential of blood volume estimation based on blood surface distance measurements, making it a promising alternative for real-time blood volume monitoring during CPB. Integrating this method into CPB systems could improve safety, prevent air embolisms, and improve fluid management, paving the way for more precise and automated circulating blood volume control.","PeriodicalId":15177,"journal":{"name":"Journal of Artificial Organs","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144078273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current status and prospects of genetically modified porcine-to-human cardiac xenotransplantation. 转基因猪-人异种心脏移植的现状与展望。

IF 1.1 4区医学

Journal of Artificial Organs Pub Date : 2025-05-04 DOI: 10.1007/s10047-025-01504-z

Takuji Kawamura, Shunsuke Saito, Takura Taguchi, Daisuke Yoshioka, Ai Kawamura, Yusuke Misumi, Takashi Yamauchi, Shuji Miyagawa, Shigeru Miyagawa

{"title":"Current status and prospects of genetically modified porcine-to-human cardiac xenotransplantation.","authors":"Takuji Kawamura, Shunsuke Saito, Takura Taguchi, Daisuke Yoshioka, Ai Kawamura, Yusuke Misumi, Takashi Yamauchi, Shuji Miyagawa, Shigeru Miyagawa","doi":"10.1007/s10047-025-01504-z","DOIUrl":"https://doi.org/10.1007/s10047-025-01504-z","url":null,"abstract":"Cardiac xenotransplantation utilizing genetically modified pigs presents a promising avenue for treating end-stage heart failure, a leading cause of mortality worldwide. This paper delineates the current landscape of heart failure treatment in Japan, emphasizing the limitations of existing therapies such as heart transplantation and implantable left ventricular assist devices. It discusses the history and advancements in the development of genetically modified pigs for xenotransplantation, highlighting recent breakthroughs and challenges. The manuscript also addresses the specific challenges facing the implementation of xenotransplantation in Japan, including the selection of suitable genetically modified pigs, ensuring organ safety, patient selection criteria, transplantation protocols, and immunosuppression strategies. Drawing from international experiences and ongoing research efforts, the paper emphasizes the potential of xenotransplantation while acknowledging the hurdles that must be overcome for widespread clinical adoption.","PeriodicalId":15177,"journal":{"name":"Journal of Artificial Organs","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differences between xenotransplantation and allogeneic kidney transplantation: the current situation and future challenges in Japan. 异种移植和异体肾移植的区别：日本的现状和未来的挑战。

IF 1.1 4区医学

Journal of Artificial Organs Pub Date : 2025-05-02 DOI: 10.1007/s10047-025-01506-x

Soichi Matsumura, Yoichi Kakuta, Yoko Maegawa-Higa, Shota Fukae, Ryo Tanaka, Shigeaki Nakazawa, Kazuaki Yamanaka, Shuji Miyagawa, Norio Nonomura

{"title":"Differences between xenotransplantation and allogeneic kidney transplantation: the current situation and future challenges in Japan.","authors":"Soichi Matsumura, Yoichi Kakuta, Yoko Maegawa-Higa, Shota Fukae, Ryo Tanaka, Shigeaki Nakazawa, Kazuaki Yamanaka, Shuji Miyagawa, Norio Nonomura","doi":"10.1007/s10047-025-01506-x","DOIUrl":"https://doi.org/10.1007/s10047-025-01506-x","url":null,"abstract":"Kidney transplantation is the only curative option for patients with chronic renal failure, significantly improving their survival and quality of life. However, this treatment remains limited by the shortage of organ donors. The shortage of kidney donors remains a serious problem all over the world, and is particularly severe in Japan. While advancements in immunosuppressive therapies and histocompatibility testing have improved outcomes in allogeneic kidney transplantation, the rising number of dialysis patients has worsened the gap between the demand for and supply of suitable donor organs. In response to this pressing need, xenotransplantation has gained attention as a promising alternative solution. Recent progress driven by gene-editing technologies, including CRISPR-Cas9, has facilitated the development of genetically modified pigs suitable for potential human transplantation. This review provides an overview of the key differences in immune response and infection risks between xenogeneic and allogeneic kidney transplants. In addition, it comprehensively examines the challenges and potential of xenogeneic kidney transplantation from multiple perspectives, including differences in immunosuppressive therapies between allogeneic and xenogeneic transplantation. We also discuss the feasibility of xenogeneic kidney transplantation as a solution to the organ shortage in Japan and present directions for addressing challenges toward clinical application. We hope this review will provide valuable insights into the potential of xenogeneic kidney transplantation as a new treatment option for chronic renal failure and contribute to efforts to address the donor shortage problem in Japan.","PeriodicalId":15177,"journal":{"name":"Journal of Artificial Organs","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144025937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Admission hematocrit and fluctuating blood urea nitrogen levels predict the efficacy of blood purification treatment in severe acute pancreatitis patients. 入院时血细胞比容和波动血尿素氮水平预测严重急性胰腺炎患者血液净化治疗的疗效。

IF 1.1 4区医学

Journal of Artificial Organs Pub Date : 2025-04-25 DOI: 10.1007/s10047-025-01501-2

Bibi Chen, Junhuang Chen, Handong Huang, Liqun Yan, Ling Lin, Hongwei Huang

{"title":"Admission hematocrit and fluctuating blood urea nitrogen levels predict the efficacy of blood purification treatment in severe acute pancreatitis patients.","authors":"Bibi Chen, Junhuang Chen, Handong Huang, Liqun Yan, Ling Lin, Hongwei Huang","doi":"10.1007/s10047-025-01501-2","DOIUrl":"https://doi.org/10.1007/s10047-025-01501-2","url":null,"abstract":"This study aimed to evaluate the prognostic significance of the levels of admission hematocrit (HCT) and the changes in the initial blood urea nitrogen (BUN) levels in predicting the efficacy of blood purification (BP) therapy in ameliorating severe acute pancreatitis (SAP) patients at admission. A retrospective study was conducted on 139 SAP patients from the People's Hospital of Guangxi Zhuang Autonomous Region from 2013 to 2022 and the data retrieved from the Medical Information Mart for Intensive Care IV (MIMIC-IV) database for 346 SAP patients. The patients were stratified based on their HCT0 levels at admission; HCT < 44% (n = 93) and HCT ≥ 44% (n = 46) and ΔBUN levels within the first 24 h post-admission; ΔBUN ≤ 0 (n = 78) and ΔBUN > 0 (n = 61). Propensity score matching (PSM) was performed on factors such as age and gender to control for differences among the strata. The clinical outcomes of the patients receiving or not receiving BP therapy were compared based on the mentioned criteria. Patients with HCT0 ≥ 44%, who were treated with BP showed no significant difference in the 28-day mortality. However, a significant increase in hospital expenses and prolonged ICU stays was observed (P < 0.05). Conversely, patients with ΔBUN ≤ 0 who received BP therapy demonstrated relatively high 28-day mortality rates, prolonged ICU stays, increased hospital expenses, and low SOFA scores (P < 0.05). The analyses of MIMIC-IV database data corroborated these findings. The predictive efficacy of BP therapy in SAP patients was significantly influenced by the changes in BUN levels at 24 h post-admission compared to the initial levels of HCT on admission. Selecting SAP patients suitable for BP treatment should be based on the changes in BUN levels to enhance effective therapeutic outcomes.","PeriodicalId":15177,"journal":{"name":"Journal of Artificial Organs","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144010936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of hybrid thoracic endovascular aortic repair for aortic arch diseases on life expectancy. 主动脉弓疾病混合胸血管内主动脉修复术对预期寿命的影响。

IF 1.1 4区医学

Journal of Artificial Organs Pub Date : 2025-04-24 DOI: 10.1007/s10047-025-01503-0

Tomoaki Kudo, Toru Kuratani, Ryoto Sakaniwa, Yoshiki Sawa, Shigeru Miyagawa

{"title":"Impact of hybrid thoracic endovascular aortic repair for aortic arch diseases on life expectancy.","authors":"Tomoaki Kudo, Toru Kuratani, Ryoto Sakaniwa, Yoshiki Sawa, Shigeru Miyagawa","doi":"10.1007/s10047-025-01503-0","DOIUrl":"https://doi.org/10.1007/s10047-025-01503-0","url":null,"abstract":"This study aimed to investigate whether the efficacy of proximal landing zones (LZs) 0, 1, and 2 in hybrid thoracic endovascular aortic repair (TEVAR) without cardiopulmonary bypass (CPB) in elderly patients can broaden aortic arch surgical eligibility from the perspective of a lifetime loss. From April 2008 to March 2020, 254 patients who underwent LZs 0, 1, and 2 hybrid TEVAR without CPB were enrolled (zone 0 in 41, zone 1 in 82, and zone 2 in 131 patients). The patients had a median age of 73 years, with a median follow-up period of 5.8 years. The median operative time was 172 min, the median postoperative hospital stay was 11 days, and the discharge rate to home was 95.3%. The survival rate, aorta-related death-free rate, and aortic events-free rate at 10 years were 72.8%, 97.0%, and 88.5%. A multivariable Cox proportional hazard regression analysis showed that age (p < 0.001), preoperative stroke (p = 0.042), and an occurrence of 30-day aortic events (p = 0.022) were significant risk factors for all-cause death. The lifetime loss for 75, 80, 85, and ≥ 90-year-old patients at the time of surgery was 0.2, 0.1, 0, and 0 years. Despite the high risk of surgical management of aortic arch diseases, this study revealed that LZs 0, 1, and 2 hybrid TEVAR without CPB in ≥ 75-year-old patients can be expected to result in a life expectancy similar to the standard life expectancy. Advanced age alone should not exclude surgical treatment for patients with arch diseases.","PeriodicalId":15177,"journal":{"name":"Journal of Artificial Organs","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144007538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Survey on Japanese emergency physicians' awareness of advanced mechanical circulatory support upgrade therapy for patients with out-of-hospital cardiac arrest receiving extracorporeal cardiopulmonary resuscitation. 日本急诊医师对院外心脏骤停接受体外心肺复苏患者先进机械循环支持升级疗法认知的调查

IF 1.1 4区医学

Journal of Artificial Organs Pub Date : 2025-04-14 DOI: 10.1007/s10047-025-01502-1

Tasuku Hada, Toru Hifumi, Shutaro Isokawa, Yasumasa Tsukamoto, Norihide Fukushima, Norio Otani

{"title":"Survey on Japanese emergency physicians' awareness of advanced mechanical circulatory support upgrade therapy for patients with out-of-hospital cardiac arrest receiving extracorporeal cardiopulmonary resuscitation.","authors":"Tasuku Hada, Toru Hifumi, Shutaro Isokawa, Yasumasa Tsukamoto, Norihide Fukushima, Norio Otani","doi":"10.1007/s10047-025-01502-1","DOIUrl":"https://doi.org/10.1007/s10047-025-01502-1","url":null,"abstract":"Destination therapy (DT) using an implantable left ventricular assist device (i-LVAD) in Japan has expanded treatment options for patients with out-of-hospital cardiac arrest (OHCA) receiving extracorporeal cardiopulmonary resuscitation (ECPR). However, achieving hemodynamic stabilization and improved peripheral organ perfusion is essential. In patients with OHCA, percutaneous mechanical circulatory support (MCS) devices may be inadequate in some cases, necessitating surgical MCS. Despite the growing importance of MCS upgrading, awareness among Japanese emergency physicians remains unclear. We aimed to assess awareness of advanced MCS upgrading strategies in refractory patients after ECPR. A nationwide survey of 293 emergency and critical care centers in Japan assessed awareness of i-LVAD therapy after ECPR. The questionnaire collected data on demographics, LVAD strategy recognition, MCS upgrade practices, and barriers. Of 90 facilities (31%) that responded, 87 (30%) consented to using their responses in the analysis. Awareness of DT was 59.8%. Notably, 66.7% and 70.1% of the respondents recognized that MCS upgrading could lead to i-LVAD treatment and heart transplantation, respectively. The major barrier to MCS upgrade therapy was the facility's inability to implement it. DT awareness was similar between IMPELLA-registered and non-registered facilities, but i-LVAD indication recognition was higher in IMPELLA-registered facilities (78.0% vs. 51.4%, P = 0.009). The low survey response rate suggests limited MCS upgrade awareness among Japanese emergency physicians. Although facilities recognize pathways to i-LVAD and transplantation, barriers to their implementation persist. IMPELLA-registered facilities showed higher i-LVAD indication recognition, reflecting their accessibility to LV unloading devices and connections with backup hospitals.","PeriodicalId":15177,"journal":{"name":"Journal of Artificial Organs","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143997581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Elucidation of the mechanism of precipitate formation on mixing nafamostat mesylate solution with dialysate. 甲磺酸那莫他酯溶液与透析液混合沉淀形成机理的探讨。

IF 1.1 4区医学

Journal of Artificial Organs Pub Date : 2025-04-08 DOI: 10.1007/s10047-025-01500-3

Iori Okamoto, Koichiro Oji, Otoka Nakashima, Yoka Shu, Hisatsugu Takahara, Hitoshi Suzuki, Hitoshi Muguruma

{"title":"Elucidation of the mechanism of precipitate formation on mixing nafamostat mesylate solution with dialysate.","authors":"Iori Okamoto, Koichiro Oji, Otoka Nakashima, Yoka Shu, Hisatsugu Takahara, Hitoshi Suzuki, Hitoshi Muguruma","doi":"10.1007/s10047-025-01500-3","DOIUrl":"https://doi.org/10.1007/s10047-025-01500-3","url":null,"abstract":"This study clarifies the reagent preparation and administration conditions that lead to precipitation when nafamostat mesylate salt (NM) solution is mixed with dialysate. The objective is to elucidate the mechanism of precipitation. It was observed that the Carbostar® dialysate forms a precipitate immediately after mixing with the NM solution, whereas Kindaly® and Subpac® dialysates form a precipitate after approximately 30 min. This variance is attributed to the pH adjusters used: citric acid for Carbostar®, acetic acid for Kindaly®, and hydrochloric acid for Subpac®. As trivalent negative ions, citrate ions promote faster precipitation compared to acetic acid and hydrochloric acid, which are monovalent ions. Nafamostat acetate and nafamostat chloride salts are soluble, while nafamostat citrate is poorly soluble, leading to differences in precipitation timing. The bicarbonate ion's pKa value is 6.1, meaning precipitation does not occur if the pH is lowered below 6.1 using acetic acid or hydrogen chloride as pH adjusters. The solubility product of nafamostat bicarbonate is 8.1 × 10-7 (mol/L)3, making precipitation inevitable under dialysis conditions ([nafamostat2+] = 5 mg/mL (9.2 mM), [HCO3-] = 35 mM). The primary precipitate component is nafamostat bicarbonate salt. Although NM is initially water-soluble, it undergoes chemical transformation into a poorly soluble salt through ion exchange from mesylate ions to bicarbonate ions within the dialysate, resulting in precipitation. Based on these findings, we propose conditions to avoid precipitation.","PeriodicalId":15177,"journal":{"name":"Journal of Artificial Organs","volume":" ","pages":""},"PeriodicalIF":1.1,"publicationDate":"2025-04-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143803246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0