Journal of biotechnology最新文献_第9页

Unlocking new frontiers in healthcare: The impact of nano-optical biosensors on personalized medical diagnostics 开启医疗保健新领域：纳米光学生物传感器对个性化医疗诊断的影响

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-15 DOI: 10.1016/j.jbiotec.2025.02.005

Bakr Ahmed Taha , Ali J. Addie , Surjeet Chahal , Adawiya J. Haider , Sarvesh Rustagi , Norhana Arsad , Vishal Chaudhary

{"title":"Unlocking new frontiers in healthcare: The impact of nano-optical biosensors on personalized medical diagnostics","authors":"Bakr Ahmed Taha , Ali J. Addie , Surjeet Chahal , Adawiya J. Haider , Sarvesh Rustagi , Norhana Arsad , Vishal Chaudhary","doi":"10.1016/j.jbiotec.2025.02.005","DOIUrl":"10.1016/j.jbiotec.2025.02.005","url":null,"abstract":"<div><div>Nano-optical biosensors have emerged as transformative tools in healthcare and clinical research, offering rapid, portable, and specific diagnostic solutions. This review critically analyzes the recent advancements, translational challenges, and sustainable approaches in nano-optical biosensor implementation for biomedical applications. We explore the integration of innovative nanomaterials, microelectronics, and molecular biology techniques that have significantly enhanced biosensor sensitivity and specificity, enabling detection of biomarkers ranging from cancer indicators to cardiovascular markers. The potential of nanoplasmonic and silicon photonic biosensors in overcoming current limitations is discussed, alongside the promising integration of artificial intelligence and Internet of Things technologies for improved data analytics and clinical validation. We address key challenges, including size constraints, energy efficiency, and integration with existing technologies, and propose sustainable strategies for eco-friendly materials, energy-efficient designs, and circular economy approaches. The review also examines emerging trends such as multiplexed sensing platforms, wearable biosensors, and their applications in personalized medicine. By critically assessing these developments, we provide insights into the prospects of nano-optical biosensors and their potential to revolutionize point-of-care diagnostics and personalized healthcare, while emphasizing the need for interdisciplinary collaboration to overcome remaining obstacles in translating these technologies from laboratory research to real-world clinical applications.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"400 ","pages":"Pages 29-47"},"PeriodicalIF":4.1,"publicationDate":"2025-02-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143427774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Waste cooking oils as a sustainable feedstock for bio-based application: A systematic review 废弃食用油作为生物基应用的可持续原料：系统综述。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-13 DOI: 10.1016/j.jbiotec.2025.02.003

Antonio Caporusso , Matteo Radice , Antonino Biundo , Ruggiero Gorgoglione , Gennaro Agrimi , Isabella Pisano

{"title":"Waste cooking oils as a sustainable feedstock for bio-based application: A systematic review","authors":"Antonio Caporusso , Matteo Radice , Antonino Biundo , Ruggiero Gorgoglione , Gennaro Agrimi , Isabella Pisano","doi":"10.1016/j.jbiotec.2025.02.003","DOIUrl":"10.1016/j.jbiotec.2025.02.003","url":null,"abstract":"<div><div>Waste cooking oils (WCOs) are common wastes and promising green, eco-friendly and sustainable feedstocks for bio-based applications. While the primary valorisation strategy revolves around the concept of waste-to energy, new research trends have emerged in the last decade. This systematic review provides a comprehensive analysis of the current state of the art in the conversion of WCOs into bio-based molecules. Based on the PRISMA methodology, 64 papers were selected using different databases and sources, such as: PubMed, ScienceDirect, Scopus and MDPI. The data extraction process focused on studies reporting the biological and chemical conversion of WCOs into value-added bioproducts. Many of the selected publications deal with the development of bioactive molecules, including biosurfactants, with application in pharmaceuticals, food, cosmetics, and bioremediation. Bioconversion processes mainly featured engineered <em>Yarrowia lipolytica</em> and <em>Escherichia coli</em> strains, even if additional microorganisms were also employed. In the same way, different chemical processes have been thoroughly studied. A smaller segment of research is directed to the production of feed supplements and soaps. Regulatory constraints limit further development in feed supplements due to potential contaminants, while soap production needs further stability studies. The present systematic review shows promising outcomes in the valorisation of WCOs through the development of value-added molecules and products. Despite the wide range of applications, these findings identify that the scalability and economic sustainability of the selected processes require further investigation. This study seeks to summarize the current state of the art and identify potential gaps to advance the industrialization of WCOs valorisation.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"400 ","pages":"Pages 48-65"},"PeriodicalIF":4.1,"publicationDate":"2025-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143425341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of a sucrose phosphorylase variant from Streptococcus mutans for production of epigallocatechin gallate glucosides 一种用于生产表没食子儿茶素没食子酸酯糖苷的变形链球菌蔗糖磷酸化酶变体的鉴定。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-11 DOI: 10.1016/j.jbiotec.2025.02.004

Xiaona Chen , Jianjian Tong , Kai Chen , Ruiqi Ma , Zhen Gao , Yan Li , Honghua Jia

{"title":"Identification of a sucrose phosphorylase variant from Streptococcus mutans for production of epigallocatechin gallate glucosides","authors":"Xiaona Chen , Jianjian Tong , Kai Chen , Ruiqi Ma , Zhen Gao , Yan Li , Honghua Jia","doi":"10.1016/j.jbiotec.2025.02.004","DOIUrl":"10.1016/j.jbiotec.2025.02.004","url":null,"abstract":"<div><div>Epigallocatechin gallate (EGCG) primarily extracted from green tea, exhibits significant antioxidant, hypolipidemic, and anticancer properties. Nevertheless, the limited aqueous solubility and bioavailability of EGCG impede its practical applications. Glycosylation modification of EGCG is an effective method for enhancing its properties. In the present study, a thermally stable variant of sucrose phosphorylase from <em>Streptococcus mutans</em> (<em>Sm</em>SP) was identified as having catalytic activity for glycosylation of EGCG. The optimal temperature and pH for <em>Sm</em>SP were determined to be 45°C and 6, respectively. The mono-glycosylation product of EGCG was structurally characterized as (−)-epigallocatechin gallate 4′-O-α-D-glucopyranoside. Under optimal reaction conditions (8 % methanol, 10 g/L EGCG, 300 g/L sucrose, 40 U/mL crude enzyme, 35°C, and 24 h), the conversion rate of EGCG reached 80.79 %. Hydrogen bonding interactions between the enzyme and ligands may enhance the stability and catalytic activity of <em>Sm</em>SP. Two active site loops significantly influence the selective formation of EGCG glycosides. These insights expand our understanding of the structural basis of sucrose phosphorylases in the synthesis of EGCG glycosides.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"401 ","pages":"Pages 21-31"},"PeriodicalIF":4.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143414394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of liquid and solid-state cultures on hemoglobin production and oxidative state in Saccharomyces cerevisiae 液体和固体培养物对酿酒酵母血红蛋白生成和氧化状态的影响。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-08 DOI: 10.1016/j.jbiotec.2025.01.020

Facundo Muñiz-Paredes , Olena P. Ishchuk , Dina Petranovic

{"title":"Impact of liquid and solid-state cultures on hemoglobin production and oxidative state in Saccharomyces cerevisiae","authors":"Facundo Muñiz-Paredes , Olena P. Ishchuk , Dina Petranovic","doi":"10.1016/j.jbiotec.2025.01.020","DOIUrl":"10.1016/j.jbiotec.2025.01.020","url":null,"abstract":"<div><div>Recombinant human hemoglobin gained attention due to its potential use as a blood-free oxygen carrier substitute. To enhance human hemoglobin production in <em>Saccharomyces cerevisiae</em>, various genetic engineering strategies have been employed, including: increasing intracellular heme levels, minimizing heme and protein degradation pathways, and co-expressing the α-hemoglobin stabilizing protein (AHSP). Solid-state culture (SSC) may enhance hemoglobin production by increasing heme biosynthesis, as it relates to intracellular oxygen availability. A comparative analysis of heme and hemoglobin production was conducted between liquid culture (LC) and SSC using the <em>S. cerevisiae</em> AHSP strain. While both systems exhibited comparable heme and hemoglobin yields per cell, a significant 18 % increase in biomass was observed in SSC. The expression of the aerobic master gene <em>HAP1</em> remained consistent between both systems, however, <em>CYC1</em> (regulated by <em>HAP1</em>) was two-fold overexpressed in SSC, indicating higher oxygen transference and possibly more efficient electron transport. Several antioxidant genes were downregulated in the SSC, suggesting that LC may be more susceptible to electron leakage during oxidative phosphorylation, potentially due to the lower expression of <em>CYC1</em>. It is proposed that high expression of antioxidant genes in LC inhibits biomass production due to the metabolic burden of maintaining redox homeostasis. These differences between LC and SSC may explain the suitability of SSC as a platform for recombinant protein production.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"400 ","pages":"Pages 1-7"},"PeriodicalIF":4.1,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143391005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Progress on production of malic acid and succinic acid by industrially-important engineered microorganisms 工业上重要的工程微生物生产苹果酸和琥珀酸的研究进展。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-07 DOI: 10.1016/j.jbiotec.2025.02.001

Na Wu , Wenxin Wang , Jianguo Zhu

{"title":"Progress on production of malic acid and succinic acid by industrially-important engineered microorganisms","authors":"Na Wu , Wenxin Wang , Jianguo Zhu","doi":"10.1016/j.jbiotec.2025.02.001","DOIUrl":"10.1016/j.jbiotec.2025.02.001","url":null,"abstract":"<div><div>Organic acids are widely used as additives in the food, pharmaceutical, chemical, and plastic industries. Currently, the industrial production methods of organic acids mainly include plant extraction and chemical synthesis. The latter mainly uses petroleum-based compounds as raw materials to synthesize organic acids through a series of chemical reactions. All of these methods have problems such as environmental pollution, high cost, and unsustainability. By contrast, microbial fermentation can effectively utilize a variety of carbon sources. Due to its low production cost, environmental friendliness, and high product purity, microbial fermentation has received increasing attention in recent years. However, the low yield and long fermentation cycle of microbial fermentation limits its industrial application. With the development of genomics, transcriptomics, and other omics technologies, the metabolic pathways of various strains producing organic acids have gradually been elucidated. Based on this, new technologies such as synthetic biology and high-throughput screening have also been extensively studied. This review summarizes the latest research progress in improving organic acid biosynthesis through metabolic engineering, focusing on L-malic acid (L-MA) and succinic acid (SA). Finally, we also discuss the challenges and future prospects of this field. This review has important reference value in the fields of food, pharmaceuticals, and chemicals, providing a theoretical basis for the study of organic acid biosynthesis.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"400 ","pages":"Pages 8-19"},"PeriodicalIF":4.1,"publicationDate":"2025-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143382432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Experimental study on the mechanism of biological hydrogen sulfide generation from organic sulfur-rich coal 有机富硫煤生物生成硫化氢机理的实验研究。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-06 DOI: 10.1016/j.jbiotec.2025.02.002

Wenjie Zhao , Xianbo Su , Weizhong Zhao , Peipei Yan , Yixuan Zhou

{"title":"Experimental study on the mechanism of biological hydrogen sulfide generation from organic sulfur-rich coal","authors":"Wenjie Zhao , Xianbo Su , Weizhong Zhao , Peipei Yan , Yixuan Zhou","doi":"10.1016/j.jbiotec.2025.02.002","DOIUrl":"10.1016/j.jbiotec.2025.02.002","url":null,"abstract":"<div><div>Whether of primary or secondary origin, the presence of hydrogen sulfide (H<sub>2</sub>S) in coalbed methane (CBM) is commonly attributed to sulfate reduction facilitated by sulfate-reducing bacteria (SRB). However, the sulfate content in high-sulfur coal is exceptionally low, insufficient to function as a substrate for sulfate-reducing bacteria (SRB). In this study, an anaerobic digestion experiment was conducted with high-organic-sulfur coal collected from the Late Permian Longtan Formation in Guangxi Province as both the carbon and sulfur sources. The formation mechanism of H<sub>2</sub>S is revealed from the evolution rules of gas components, liquid organic matter, and microbial communities during the anaerobic digestion process. The findings indicate three distinct mechanisms contributing to the biological formation of H<sub>2</sub>S in coal seams: firstly, the degradation of readily degradable organic sulfur in coal by microorganisms possessing denitrification capabilities, primarily attributed to the activity of the <em>Wolinella</em>; secondly, The synergistic consortium involving SRB, Pseudomonas spp., and denitrifying Thiobacillus species mediates SO<sub>4</sub><sup>2-</sup> reduction and H₂S biogenesis through cross-metabolic interactions; thirdly, Methylotrophic methanogens employ the methyl groups of organic sulfides to produce CH<sub>4</sub> and H<sub>2</sub>S simultaneously. Therefore, biological H<sub>2</sub>S can be generated under the presence of a sulfur source, appropriate temperature, and conducive environmental conditions. This comprehension will contribute valuable insights to the discourse on the generation and enrichment patterns of H<sub>2</sub>S in natural coalbed methane. Additionally, it can offer practical avenues for the prevention and control of H<sub>2</sub>S through technological approaches.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"400 ","pages":"Pages 20-28"},"PeriodicalIF":4.1,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient production of spermidine from Bacillus amyloliquefaciens by enhancing synthesis pathway, blocking degradation pathway and increasing precursor supply 解淀粉芽孢杆菌通过增强合成途径、阻断降解途径和增加前体供应来高效生产亚精胺。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-01 DOI: 10.1016/j.jbiotec.2024.12.001

Ziyue Zhao, Ailing Guo, Dian Zou, Zhou Li, Xuetuan Wei

{"title":"Efficient production of spermidine from Bacillus amyloliquefaciens by enhancing synthesis pathway, blocking degradation pathway and increasing precursor supply","authors":"Ziyue Zhao, Ailing Guo, Dian Zou, Zhou Li, Xuetuan Wei","doi":"10.1016/j.jbiotec.2024.12.001","DOIUrl":"10.1016/j.jbiotec.2024.12.001","url":null,"abstract":"<div><div>Spermidine has broad application potential in food, medicine and other fields. In this study, a novel <em>Bacillus amyloliquefaciens</em> cell factory was constructed for production of spermidine from renewablebiomass resources. Firstly, the <em>speB</em> gene was found to be optimal for synthesis of spermidine, and the function of SpeB was explained by amino acid sequence analysis and molecular docking. By replacing the native promoter of the <em>speEB</em> operon with the P43, the synthesis of spermidine was significantly enhanced in <em>B. amyloliquefaciens</em> HSPM1-P43<em>speEB</em>. After knockout of the genes <em>yobN</em> and <em>bltD</em> associated with spermidine degradation, the spermidine titer of the strain HSPM2 was further improved to 115.96 mg/L, increased by 108 % compared to HSPM1-P43<em>speEB</em>. Subsequently, the titer of spermidine was further increased to 277.47 mg/L through enhancing the supply of the precursor methionine by overexpression of <em>speD</em>. Finally, the renewable biomass resources, xylose and feather meal were optimized to produce spermidine, and the maximum titer is up to 588.10 mg/L after optimization. In conclusion, an efficient spermidine producing <em>B. amyloliquefaciens</em> was constructed through combinatorial metabolic engineering strategies, and the sustainable production of spermidine was achieved using the biomass resources of xylose and feather meal.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"398 ","pages":"Pages 87-96"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142794715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review towards sustainable analyte detection: Biomimetic inspiration in biosensor technology 可持续分析物检测综述：生物传感器技术的仿生启示。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-01 DOI: 10.1016/j.jbiotec.2024.11.015

Pratistha Bhagat , Lata Sheo Bachan Upadhyay

{"title":"A review towards sustainable analyte detection: Biomimetic inspiration in biosensor technology","authors":"Pratistha Bhagat , Lata Sheo Bachan Upadhyay","doi":"10.1016/j.jbiotec.2024.11.015","DOIUrl":"10.1016/j.jbiotec.2024.11.015","url":null,"abstract":"<div><div>The branch of biomimetics has witnessed a profound impact on the field of biosensor technology, reflected in sustainable analyte detection. A vast array of biosensor platforms with improved/upgraded performance have been developed and reported. No wonder the motivation from the field of biomimetics has a huge impact on generating detection systems with escalated degrees of manipulation and tunability at different levels. More recently, biomimetic biosensor technology has found potential in constructing bio-inspired materials such as aptamers, MIPs, nanozymes, DNAzymes, Synzymes, etc. to be integrated with biosensor fabrication. The establishment of a sensing setup is not limited to the bioreceptor fabrication; the construction of transducing element using biomimetic material have been reported too. Moreover, to serve a biosensing of target analyte from a fatal diseased sample different biomimetic architectures can be designed that mimic in-vivo microenvironmental surroundings to get an exact microenvironment equivalent to natural conditions leading towards designing of a precise treatment strategy. This research area is ever-evolving as there is a scope for upgradation and refinement due to advancing technologies including nanotechnology, biomimetic nanomaterials, microfluidics, optical sensors, etc. This review is an attempt to comprehend and juxtapose the very primary innovations in the field of biomimetic biosensor technology to realize its comprehensive and wide-range scope and possibilities.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"398 ","pages":"Pages 51-65"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinformatics-assisted mining and design of novel pullulanase suitable for starch cold hydrolysis 生物信息学辅助下适用于淀粉冷水解的新型普鲁兰酶的挖掘与设计。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-01 DOI: 10.1016/j.jbiotec.2024.12.005

Xin Wang , Zixing Wang , Xueting Zhang , Yanli Zhang , Wenxia Zhang , Yu Zhang , Xuecheng Zhang , Yazhong Xiao , Yinliang Zhang , Wei Fang

{"title":"Bioinformatics-assisted mining and design of novel pullulanase suitable for starch cold hydrolysis","authors":"Xin Wang , Zixing Wang , Xueting Zhang , Yanli Zhang , Wenxia Zhang , Yu Zhang , Xuecheng Zhang , Yazhong Xiao , Yinliang Zhang , Wei Fang","doi":"10.1016/j.jbiotec.2024.12.005","DOIUrl":"10.1016/j.jbiotec.2024.12.005","url":null,"abstract":"<div><div>Cold-active pullulanases with good catalytic performance possess promising applications in cold hydrolysis of starch. Adopting bioinformatics-assisted mining strategies, 7 candidate cold-active pullulanases were initially screened out from IMG/MER database. Among the candidates, PulBs exhibited good thermostability and the highest specific activity of 147.4 U/mg. The half-life of PulBs was about 200 h at 35 °C. Employing PulBs as the initial enzyme, the active-site design of FuncLib was implemented to enhance the activity. The design PulBs-20 exhibited an enhanced specific activity of 209.9 U/mg, which was 1.4 times that of PulBs. Furthermore, the thermostability of PulBs-20 was augmented, with a half-life of 250 h at 35 °C. When applied in the cold hydrolysis of starch, PulBs-20 can effectively enhance the hydrolysis effect of raw starch. Supplemented with the raw starch-hydrolyzing α-amylase AmyZ1 and PulBs-20, the hydrolysis rate of raw corn starch increased to 53.5 %, which was 1.3 times that of using AmyZ1 alone. Due to its high hydrolysis activity and good thermostability, PulBs-20 can serve as an efficient accessory enzyme in starch cold hydrolysis.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"398 ","pages":"Pages 106-116"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142835780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Cell-free reduction of carboxylic acids with secreted carboxylic acid reductase” [J. Biotechnol. 382 (2024) 44–50] 用分泌的羧酸还原酶进行无细胞羧酸还原的勘误表[J]。生物工程学报，2016,44(2):444 - 451。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-01 DOI: 10.1016/j.jbiotec.2024.12.002

Dominic Goj , Stella Ebner , Melissa Horvat , Simon Arhar , Ludmila Martínková , Margit Winkler

引用次数: 0