Journal of biotechnology最新文献

{"title":"System-wide analysis of groundnut's salinity resilience: Integrating plant-cell interactions with environmental stress dynamics through cutting-edge transcriptomics","authors":"Meera K. Joshi ,&nbsp;Gopal V. Marviya ,&nbsp;Feba Jacob ,&nbsp;Umesh K. Kandoliya ,&nbsp;Priyanka M. Pandya ,&nbsp;Ashish G. Vala","doi":"10.1016/j.jbiotec.2024.07.023","DOIUrl":"10.1016/j.jbiotec.2024.07.023","url":null,"abstract":"<div><p>Salinity stress is a major concern in regions where irrigation relies on saline water. This study aimed to investigate the relative water content (RWC), electrolytic leakage (EL), total chlorophyll content, free amino acid content, and total soluble sugar content were analyzed in different groundnut species subjected to various salinity treatments. The results showed that salinity stress significantly reduced the RWC in groundnut leaves, with <em>A. duranensis</em> (wild type) exhibiting higher RWC values compared to the <em>Arachis hypogaea</em> species. RNA sequencing was performed to identify differentially expressed genes (DEGs) during salt stress. A total of 9079 DEGs were identified, with 1372 genes upregulated and 2509 genes downregulated. Genes belonging to transcription factor families, such as WRKY, MYB, bHLH, E2F, and Auxin efflux carrier proteins, were induced under salt stress in the tolerant genotype. Conversely, genes encoding NADH dehydrogenase, glutathione S-transferase, protein kinases, UDP-glycosyltransferase, and peroxidase were downregulated. Gene ontology and pathway analyses revealed several enriched categories and metabolic pathways associated with salt stress response, including catalytic activity, response to salt stress, ATP-dependent activity, and oxidative phosphorylation. The findings of this study provide insights into the physiological and molecular responses of groundnut to salinity stress. <em>A. duranensis</em> exhibited better salinity tolerance than <em>Arachis hypogaea</em>, as indicated by higher RWC values, lower electrolytic leakage, and differential gene expression patterns. These results contribute to our understanding of the mechanisms underlying salt stress tolerance in groundnut and may guide future efforts to develop salinity-tolerant groundnut species, ultimately improving crop yield in saline-affected regions.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"394 ","pages":"Pages 34-47"},"PeriodicalIF":4.1,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carboligation towards production of hydroxypentanones","authors":"Hana Dobiašová ,&nbsp;Valentina Jurkaš ,&nbsp;Frederika Kabátová ,&nbsp;Melissa Horvat ,&nbsp;Florian Rudroff ,&nbsp;Kvetoslava Vranková ,&nbsp;Peter Both ,&nbsp;Margit Winkler","doi":"10.1016/j.jbiotec.2024.08.004","DOIUrl":"10.1016/j.jbiotec.2024.08.004","url":null,"abstract":"<div><p>2-Hydroxy-3-pentanone and 3-hydroxy-2-pentanone are flavor molecules present in various foods, such as cheese, wine, durian, and honey, where they impart buttery, hay-like, and caramel-sweet aromas. However, their utilization as flavoring agents is constrained by a lack of developed synthesis methods. In this study, we present their synthesis from simple starting compounds available in natural quality, catalyzed by previously characterized ThDP-dependent carboligases. Additionally, we demonstrate that newly discovered homologues of pyruvate dehydrogenase from <em>E. coli</em> (<em>Ec</em>PDH E1), namely <em>La</em>PDH from <em>Leclercia adecarboxylata</em>, <em>Cn</em>PDH from <em>Cupriavidus necator</em>, and <em>Tc</em>PDH from <em>Tanacetum cinerariifolium</em>, exhibit promising potential for α-hydroxy pentanone synthesis in form of whole-cell biocatalysts. Enzyme stability at varying pH levels, kinetic parameters, and reaction intensification were investigated. <em>Cn</em>PDH, for example, exhibits superior stability across different pH levels compared to <em>Ec</em>PDH E1. Both α-hydroxy pentanones can be produced with <em>Cn</em>PDH in satisfactory yields (74% and 59%, respectively).</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"393 ","pages":"Pages 161-169"},"PeriodicalIF":4.1,"publicationDate":"2024-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168165624002141/pdfft?md5=0dfec778895df0278f5046cc0fadada4&pid=1-s2.0-S0168165624002141-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141912803","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Orthogonal characterization of rAAV9 reveals unexpected transgene heterogeneity","authors":"Peter Eisenhut ,&nbsp;Peter Andorfer ,&nbsp;Andrea Haid ,&nbsp;Beatrice Jokl ,&nbsp;Raffaela Manhartsberger ,&nbsp;Felix Fuchsberger ,&nbsp;Bernd Innthaler ,&nbsp;Johannes Lengler ,&nbsp;Barbara Kraus ,&nbsp;Robert Pletzenauer ,&nbsp;Juan A. Hernandez Bort ,&nbsp;Sabine Unterthurner","doi":"10.1016/j.jbiotec.2024.07.020","DOIUrl":"10.1016/j.jbiotec.2024.07.020","url":null,"abstract":"<div><p>Recombinant adeno-associated virus (rAAV) is the most widely used viral vector for <em>in vivo</em> human gene therapy. To ensure safety and efficacy of gene therapy products, a comprehensive analytical profile of the rAAVs is needed, which provides crucial information for therapeutic development and manufacturing. Besides information on rAAV quantities and possible contaminating DNA and protein species, assessing rAAV quality is of utmost importance. <em>In vitro</em> biopotency and methods to determine the full/empty ratio of rAAV capsids are commonly applied, but methods to assess the integrity of the viral genome are still rarely used. Here we describe an orthogonal approach to characterize rAAV quality. Two biologically different rAAV9s from different stages of the bioprocess, generated each with two different transfection reagents, were investigated. <em>In vitro</em> biopotency tests in all cases demonstrated that rAAV9s generated with transfection reagent FectoVIR® possessed a higher biological activity. Mass-based analytical methods, such as sedimentation velocity analytical ultracentrifugation (AUC) and mass photometry, showed a high share of full capsids (&gt;80 %) at late process stages but did not detect any differences in the rAAV9s from the different transfection reagents. Multiplex dPCR and Nanopore long-read sequencing both demonstrated that, also in late-stage process samples, sample heterogeneity was relatively high with a rather small share of full-length transgenes of ∼10–40 %. Intriguingly, both methods detected a higher share of complete transgenes in rAAV9 generated with transfection reagent FectoVIR® instead of Polyethylenimine (PEI), and thereby explain the differences already observed in the biopotency assays. This study therefore emphasizes the necessity to utilize multiple, orthogonal methods to gain a better understanding of recombinantly manufactured AAVs.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"393 ","pages":"Pages 128-139"},"PeriodicalIF":4.1,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168165624002074/pdfft?md5=69aa4a0ceab6cfa3f144ca9fefa5566a&pid=1-s2.0-S0168165624002074-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141897526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Co-expression of human sialyltransferase improves N-glycosylation in Leishmania tarentolae and optimizes the production of humanized therapeutic glycoprotein IFN-beta","authors":"Renato Lima Senra ,&nbsp;Higor Sette Pereira ,&nbsp;Luana Maria Pacheco Schittino ,&nbsp;Patrícia Pereira Fontes ,&nbsp;Tatiana Aparecida de Oliveira ,&nbsp;Andrea de Oliveira Barros Ribon ,&nbsp;Juliana Lopes Rangel Fietto ,&nbsp;Liza Figueiredo Felicori Vilela ,&nbsp;Jacqueline Araújo Fiúza ,&nbsp;Tiago Antônio de Oliveira Mendes","doi":"10.1016/j.jbiotec.2024.08.002","DOIUrl":"10.1016/j.jbiotec.2024.08.002","url":null,"abstract":"<div><p>The production of therapeutic glycoproteins is primarily expensive due to the necessity of culturing mammalian cells. These systems often require complex and costly culture media and typically yield low amounts of protein. <em>Leishmania tarentolae</em>, a non-pathogenic protozoan to mammals, has emerged as a cost-effective alternative system for heterologous glycoprotein expression due to its suitability for large-scale production using low-cost culture media, and its ability to perform mammalian-like post-translational modifications, including glycosylation. Nevertheless, differences in the carbohydrate residues at the end of N-glycan chains are observed in <em>Leishmania</em> compared to mammalian cells due to the absence of biosynthetic enzymes in <em>Leishmania</em> that are required for the incorporation of terminal sialic acid. In this study, a genetically optimized <em>L. tarentolae</em> cell line was engineered for the production of recombinant interferon-β (IFN-β) featuring a complete mammalian N-glycosylation profile. Genomic and metabolomic analyses revealed that heterologous expression of the sialyltransferase enzyme and cultivation in a medium containing sialic acid were sufficient to generate mammalian-like protein N-glycosylation. N-glycan mass spectrometry analysis demonstrated a glycosylation pattern compatible with the incorporation of sialic acid into the glycan structure. In vitro IFN-β activity indicated that the expressed protein exhibited reduced inflammatory effects compared to IFN-beta produced by other platforms, such as bacteria, non-optimized <em>L. tarentolae</em>, and mammalian cells.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"394 ","pages":"Pages 24-33"},"PeriodicalIF":4.1,"publicationDate":"2024-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141893501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a 2A peptide-based multigene expression system and its application for enhanced production of ganoderic acids in Ganoderma lucidum","authors":"Qiong Wang ,&nbsp;Hong-Jun Liu ,&nbsp;Yan Xu,&nbsp;Zi-Xu Wang,&nbsp;Bin Sun,&nbsp;Jun-Wei Xu","doi":"10.1016/j.jbiotec.2024.08.001","DOIUrl":"10.1016/j.jbiotec.2024.08.001","url":null,"abstract":"<div><p><em>Ganoderma</em> has received much attention for its medicinal value, but the manipulation of multiple genes remains a challenge, hindering the genetic engineering of this species for the development of cell factories. Here, we first showed that the presence of an intron is necessary for the efficient expression of the endogenous cDNA of carboxin-resistant gene (<em>cbx</em>) in <em>G. lucidum</em>. Then, the self-cleaving function of 2 A peptide was investigated in <em>G. lucidum</em> by linking <em>cbx</em> cDNA to the codon-optimized hygromycin B-resistant gene (op<em>hph</em>) using the 2A-peptide sequence. The results showed that <em>cbx</em> cDNA and op<em>hph</em> can be successfully expressed in <em>G. lucidum</em> in a bicistronic manner from a single transcript. Moreover, the expression of both genes was not affected by the order within the 2 A cassette. In addition, simultaneous expression of <em>cbx</em> cDNA, op<em>hph</em>, and codon-optimized yellow fluorescent protein gene (op<em>yfp</em>) was conducted for the first time in <em>G. lucidum</em> using the 2 A peptide-based approach. The developed method was successfully applied to express both cDNA of the 3-hydroxy-3-methylglutaryl coenzyme A reductase (<em>hmgr</em>) and squalene epoxidase gene (<em>se</em>) for enhanced production of ganoderic acids (GAs) in <em>G. lucidum</em>. The engineered strain produced the maximum content of GA-Mk, GA-T, GA-S, and GA-Me were 26.56±3.53,39.58±3.75, 16.54±2.16, and 19.1±1.87 μg/100 mg dry weight, respectively. These values were 3.85-, 4.74-, 3.65-, and 3.23-fold higher than those produced by the control strain. The developed method will be useful for the manipulation of complex metabolic or regulatory pathways involving multiple genes in <em>Ganoderma</em>.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"393 ","pages":"Pages 109-116"},"PeriodicalIF":4.1,"publicationDate":"2024-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141893502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient synthesis of 5-hydroxytryptophan in Escherichia coli by bifunctional utilization of whey powder as a substrate for cell growth and inducer production","authors":"Bowen Shen ,&nbsp;Lin Zhang ,&nbsp;Yu Zhou ,&nbsp;Feifei Song ,&nbsp;Shengping You ,&nbsp;Rongxin Su ,&nbsp;Wei Qi","doi":"10.1016/j.jbiotec.2024.07.021","DOIUrl":"10.1016/j.jbiotec.2024.07.021","url":null,"abstract":"<div><p>5-Hydroxytryptophan (5-HTP), a precursor of the neurotransmitter serotonin in mammals, has demonstrated efficacy in treating various diseases such as depression, fibromyalgia and obesity. However, conventional biosynthesis methods of 5-HTP are limited by low yield and high reagent and process costs. In this study, the strain C1T7-S337A/F318Y with optimized promoter distribution was obtained, and the 5-HTP yield was 60.30 % higher than that of the initial strain. An efficient fermentation process for 5-HTP synthesis was developed using strain C1T7-S337A/F318Y with whey powder as a substrate for cell growth and inducer production. Shake flask fermentation experiments yielded 1.302 g/L 5-HTP from 2.0 g/L L-tryptophan (L-Trp), surpassing the whole-cell biocatalysis by 42.86 %. Scale-up to a 5 L fermenter further increased the yield to 1.649 g/L. This fermentation strategy substantially slashed reagent cost by 95.39 %, providing a more economically viable and environmentally sustainable route for industrial biosynthesis of 5-HTP. Moreover, it contributes to the broader utilization of whey powder in various industries.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"393 ","pages":"Pages 100-108"},"PeriodicalIF":4.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889330","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic whole-cell biocatalyst based on intracellular lipases of Candida catenulata as promising technology for green synthesis of epoxy fatty acids","authors":"Elham Tohfegar,&nbsp;Alireza Habibi","doi":"10.1016/j.jbiotec.2024.07.019","DOIUrl":"10.1016/j.jbiotec.2024.07.019","url":null,"abstract":"<div><p>This study focuses on the development a green synthesis of epoxy fatty acids (EFAs) which are commonly used as the plasticizer in polymer industries. The intracellular lipases of <em>Candida catenulata</em> cells as a whole-cell biocatalyst (WCB) were examined in the bio-epoxidation of free fatty acids (FFAs) with hydrogen peroxide. The FFAs in soybean soap stock, an industrial by-product of vegetable oil factories, was used as the feedstock of the process. To remove phosphates from soap stock a degumming process was tested before the bio-epoxidation reaction and results revealed that the EFAs yield was improved using the degummed fatty acids (DFAs). The attachments of magnetic Fe₃O₄ nanoparticles to the surface of WCBs facilitated the recovery of the biocatalyst, and were improved stabilities. The activation energy for the magnetic whole-cell biocatalysts (MWCB) was 48.54 kJ mol⁻¹, which was lower than the WCB system (51.28 kJ mol⁻¹). The EFA yield was about 47.1 % and 33.8 % after 3 h for the MWCBs and 2 h for the WCBs, respectively. The MWCBs displayed acceptable reusability in the repetitious bio-epoxidation reaction with maintaining 59 % of the original activity after 5 cycles whereas the performance of the WCBs was 5.9 % at the same conditions. The effects of influential factors such as reaction time, molar ratio of H₂O₂ to C<img>C, and batch and semi-batch operations were investigated for both biocatalyst systems. The quality of EFAs was characterized by FTIR and GC-MS analyses.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"393 ","pages":"Pages 117-127"},"PeriodicalIF":4.1,"publicationDate":"2024-08-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141889329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Progress in application of cyclic single-stranded nucleic acids","authors":"Xin-yang Liu ,&nbsp;Jian-fei Tong ,&nbsp;Ming-yang Li ,&nbsp;Lian-fang Li ,&nbsp;Wen-wei Cai ,&nbsp;Jin-qian Li ,&nbsp;Liang-hua Wang ,&nbsp;Ming-juan Sun","doi":"10.1016/j.jbiotec.2024.07.017","DOIUrl":"10.1016/j.jbiotec.2024.07.017","url":null,"abstract":"<div><p>Cyclic nucleic acids are biologically stable against nucleic acid exonucleases due to the absence of 5′ and 3′ termini. Studies of cyclic nucleic acids mainly focus on cyclic single-stranded nucleic acids. Cyclic single-stranded nucleic acids are further divided into circular RNA (circRNA) and circular single-stranded DNA (cssDNA). The synthesis methods of circRNA include lasso-driven cyclization, intron-paired cyclization, intron cyclization, intron complementary pairing-driven cyclization, RNA-binding protein-driven cyclization, and artificial synthesis depending on the source. Its main role is to participate in gene expression and the treatment of some diseases. Circular single-stranded DNA is mainly synthesized by chemical ligation, template-directed enzyme ligation, and new techniques for the efficient preparation of DNA single loops and topologies based on CircLigase. It is mainly used in rolling circle amplification (RCA) technology and in the bioprotection of circular aptamers and second messengers. This review focuses on the types, synthesis methods, and applications of cyclic single-stranded nucleic acids, providing a reference for further research on cyclic single-stranded nucleic acids.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"393 ","pages":"Pages 140-148"},"PeriodicalIF":4.1,"publicationDate":"2024-07-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring genetic codon expansion for unnatural amino acid incorporation in filamentous fungus Aspergillus nidulans","authors":"Xueying Li ,&nbsp;Jing Wang ,&nbsp;Jingyi Li ,&nbsp;Yao Zhou ,&nbsp;Xiaofei Huang ,&nbsp;Lingyan Guo ,&nbsp;Renning Liu ,&nbsp;Yiqing Luo ,&nbsp;Xinyu Tan ,&nbsp;Xiaotao Hu ,&nbsp;Yan Gao ,&nbsp;Bingzi Yu ,&nbsp;Mingxin Fu ,&nbsp;Ping Wang ,&nbsp;Shengmin Zhou","doi":"10.1016/j.jbiotec.2024.07.018","DOIUrl":"10.1016/j.jbiotec.2024.07.018","url":null,"abstract":"<div><p>Genetic code expansion technology allows the incorporation of unnatural amino acids (UAAs) into proteins, which is useful in protein engineering, synthetic biology, and gene therapy. Despite its potential applications in various species, filamentous fungi remain unexplored. This study aims to address this gap by developing these techniques in <em>Aspergillus nidulans</em>. We introduced an amber stop codon into a specific sequence within the reporter gene expressed in <em>A. nidulans</em> and replaced the anticodon of the fungal tRNA^Tyr with CUA. This resulted in the synthesis of the target protein, confirming the occurrence of amber suppression in the fungus. When exogenous <em>E. coli</em> tRNA^Tyr_CUA (Ec. tRNA^Tyr_CUA) and <em>E. coli</em> tyrosyl-tRNA (Ec.TyrRS) were introduced into <em>A. nidulans</em>, they successfully synthesized the target protein via amber suppression and were shown to be orthogonal to the fungal translation system. By replacing the wild-type Ec.TyrRS with a mutant with a higher affinity for the UAA <em>O</em>-methyl-L-tyrosine, the fungal system was able to initiate the synthesis of the UAA-labeled protein (UAA-protein). We further increased the expression level of the UAA-protein through several rational modifications. The successful development of a genetic code expansion technique for <em>A. nidulans</em> has introduced a potentially valuable approach to the study of fungal protein structure and function.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"393 ","pages":"Pages 91-99"},"PeriodicalIF":4.1,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788142","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure changes of lignin and their effects on enzymatic hydrolysis for bioethanol production: a focus on lignin modification","authors":"Jinju Hou ,&nbsp;Qiuzhuo Zhang ,&nbsp;Fuxiang Tian ,&nbsp;Fuwen Liu ,&nbsp;Jingxian Jiang ,&nbsp;Jiaolong Qin ,&nbsp;Huifeng Wang ,&nbsp;Jing Wang ,&nbsp;Shufang Chang ,&nbsp;Xiaojun Hu","doi":"10.1016/j.jbiotec.2024.07.012","DOIUrl":"10.1016/j.jbiotec.2024.07.012","url":null,"abstract":"<div><p>Enzymatic hydrolysis contributes to obtaining fermentable sugars using pretreated lignocellulose materials for bioethanol generation. Unfortunately, the pretreatment of lignocellulose causes low substrate enzymatic hydrolysis, which is due to the structure changes of lignin to produce main phenolic by-products and non-productive cellulase adsorption. It is reported that modified lignin enhances the speed of enzymatic hydrolysis through single means to decrease the negative effects of fermentation inhibitors or non-productive cellulase adsorption. However, a suitable modified lignin should be selected to simultaneously reduce the fermentation inhibitors concentration and non-productive cellulase adsorption for saving resources and maximizing the enzymatic hydrolysis productivity. Meanwhile, the adsorption micro-mechanisms of modified lignin with fermentation inhibitors and cellulase remain elusive. In this review, different pretreatment effects toward lignin structure, and their impacts on subsequent enzymatic hydrolysis are analyzed. The main modification methods for lignin are presented. Density functional theory is used to screen suitable modification methods for the simultaneous reduction of fermentation inhibitors and non-productive cellulase adsorption. Lignin-fermentation inhibitors and lignin-cellulase interaction mechanisms are discussed using different advanced analysis techniques. This article addresses the gap in previous reviews concerning the application of modified lignin in the enhancement of bioethanol production. For the first time, based on existing studies, this work posits the hypothesis of applying theoretical simulations to screen efficient modified lignin-based adsorbents, in order to achieve a dual optimization of the detoxification and saccharification processes. We aim to improve the integrated lignocellulose transformation procedure for the effective generation of cleaner bioethanol.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"393 ","pages":"Pages 61-73"},"PeriodicalIF":4.1,"publicationDate":"2024-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141788144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}