Journal of biotechnology最新文献

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In vitro response of sugarcane (Saccharum spp. Hybrid) plantlets to flooding stress 甘蔗(蔗属杂交种)小苗对洪水胁迫的离体反应。
IF 4.1 2区 生物学
Journal of biotechnology Pub Date : 2024-07-14 DOI: 10.1016/j.jbiotec.2024.07.003
José Luis Spinoso-Castillo , Eucario Mancilla-Álvarez , Jericó Jabín Bello-Bello
{"title":"In vitro response of sugarcane (Saccharum spp. Hybrid) plantlets to flooding stress","authors":"José Luis Spinoso-Castillo ,&nbsp;Eucario Mancilla-Álvarez ,&nbsp;Jericó Jabín Bello-Bello","doi":"10.1016/j.jbiotec.2024.07.003","DOIUrl":"10.1016/j.jbiotec.2024.07.003","url":null,"abstract":"<div><p>Flooding caused by climate change puts the productivity of sugarcane cultivation at risk. The objective of this study was to evaluate the effect of <em>in vitro</em> flooding stress on sugarcane plantlets. Sugarcane plantlets were grown in test tubes containing Murashige and Skoog semi-solid medium without growth regulators as a control treatment and two stress levels using a double layer with sterile distilled water to simulate hypoxia and anoxia. After 15 d of culture, the number of new shoots, plantlet height, number of leaves, number of roots, root length, stomatal density, percentage of closed stomata and percentage of dry matter were evaluated. In addition, biochemical variables such as chlorophylls, carotenoids, phosphoenolpyruvate (PEP), Rubisco, total proteins (TP), proline (Pr), glycine-betaine (GB), phenols, antioxidant capacity and lipid peroxidation were determined in all treatments. Results showed a higher number of new shoots, leaves and percentage of closed stomata in the flooded plantlets, while plantlet height, number of roots, stomatal density, and dry matter were higher in the control treatment. Regarding, chlorophyll, carotenoid, PEP and Rubisco contents decreased in the flooded treatments, while TP and phenol contents were higher in the partially submerged treatment. Antioxidant capacity and lipid peroxidation increased in the fully submerged treatment. Pr and GB contents did not show changes in any of the evaluated treatments. Stress induced by excess water in a double layer <em>in vitro</em> is an alternative method to determining physiological and biochemical mechanisms of tolerance to hypoxia and anoxia caused by flooding for breeding programs in sugarcane.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"393 ","pages":"Pages 74-80"},"PeriodicalIF":4.1,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141603630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multifactorial optimization enables the identification of a greener method to produce (+)-nootkatone 通过多因素优化,确定了生产 (+)-nootkatone 的更环保方法。
IF 4.1 2区 生物学
Journal of biotechnology Pub Date : 2024-07-14 DOI: 10.1016/j.jbiotec.2024.07.008
Ida M. Makhubela , Alexander Zawaira , Dean Brady , Daniel P. Pienaar
{"title":"Multifactorial optimization enables the identification of a greener method to produce (+)-nootkatone","authors":"Ida M. Makhubela ,&nbsp;Alexander Zawaira ,&nbsp;Dean Brady ,&nbsp;Daniel P. Pienaar","doi":"10.1016/j.jbiotec.2024.07.008","DOIUrl":"10.1016/j.jbiotec.2024.07.008","url":null,"abstract":"<div><p>The natural aroma compound (+)-nootkatone was obtained in selective conversions of up to 74 mol% from inexpensive (+)-valencene substrate by using a comparatively greener biocatalytic process developed based on modifications of the previously published Firmenich method. Buffer identity and concentration, pH, temperature and downstream work-up procedures were optimized to produce a crude product in which &gt;90 % of (+)-valencene had been converted, with high chemoselectivity observed for (+)-nootkatone production. Interestingly, the biotransformation was carried out efficiently at temperatures as low as 21 ºC. Surprisingly, the best results were obtained when an acidic pH in the range of 3–6 was applied, as compared to the previously published procedure in which it appeared to be necessary to buffer the pH optimally and fixed throughout at 8.5. Furthermore, there was no need to maintain a pure oxygen atmosphere to achieve good (+)-nootkatone yields. Instead, air bubbled continuously at a low rate through the reaction mixture <em>via</em> a submerged glass capillary was sufficient to enable the desired lipoxygenase-catalyzed oxidation reactions to occur efficiently. No valencene epoxide side-products were detected in the organic product extract by a standard GCMS protocol. Only traces of the anticipated corresponding α- and β-nootkatol intermediates were routinely observed.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"393 ","pages":"Pages 41-48"},"PeriodicalIF":4.1,"publicationDate":"2024-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168165624001949/pdfft?md5=1bac3a2b5792cdf7e9e8c679248ccbb6&pid=1-s2.0-S0168165624001949-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141616444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Comparing Genome Scale Metabolic Models of the non-resistant Enterococcus faecalis ATCC 19433 and the multi-resistant Enterococcus faecalis V583 比较非耐药粪肠球菌 ATCC 19433 和多重耐药粪肠球菌 V583 的基因组规模代谢模型。
IF 4.1 2区 生物学
Journal of biotechnology Pub Date : 2024-07-10 DOI: 10.1016/j.jbiotec.2024.07.006
Seyed Babak Loghmani , Eric Zitzow , Luisa Schwarzmüller , Yvonne Humboldt , Philip Eisenberg , Bernd Kreikemeyer , Nadine Veith , Ursula Kummer , Tomas Fiedler
{"title":"Comparing Genome Scale Metabolic Models of the non-resistant Enterococcus faecalis ATCC 19433 and the multi-resistant Enterococcus faecalis V583","authors":"Seyed Babak Loghmani ,&nbsp;Eric Zitzow ,&nbsp;Luisa Schwarzmüller ,&nbsp;Yvonne Humboldt ,&nbsp;Philip Eisenberg ,&nbsp;Bernd Kreikemeyer ,&nbsp;Nadine Veith ,&nbsp;Ursula Kummer ,&nbsp;Tomas Fiedler","doi":"10.1016/j.jbiotec.2024.07.006","DOIUrl":"10.1016/j.jbiotec.2024.07.006","url":null,"abstract":"<div><p><em>Enterococcus faecalis</em> is a versatile lactic acid bacterium with a large variety of implications for humans. While some strains of this species are pathobionts being resistant against most of the common antibiotics, other strains are regarded as biological protectants or even probiotics. Accordingly, <em>E. faecalis</em> strains largely differ in the size and content of their accessory genome. In this study, we describe the genome-scale metabolic network reconstruction of <em>E. faecalis</em> ATCC 19433, a non-resistant human-associated strain. A comparison of the genome-scale metabolic model (GSM) of <em>E. faecalis</em> ATCC 19433 with a previously published GSM of the multi-resistant pathobiontic <em>E. faecalis</em> V583 reveals high similarities in the central metabolic abilities of these two human associated strains. This is reflected, e.g., in the identical amino acid auxotrophies. The ATCC 19433 strain, however, has a 14.1% smaller genome than V583 and lacks the multiple antibiotic resistance genes and genes involved in capsule formation. Based on the measured metabolic fluxes at different growth rates, the energy demand at zero growth was calculated to be about 40% lower for the ATCC 19433 strain compared to V583. Furthermore, the ATCC 19433 strain seems less prone to the depletion of amino acids utilizable for energy metabolism. This might hint at a lower overall energy demand of the ATCC 19433 strain as compared to V583.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"392 ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0168165624001925/pdfft?md5=1ba001f61d78da3ee6273ca114eeb527&pid=1-s2.0-S0168165624001925-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141599917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Investigation on solvent-free esterification of oleic acid by hemp tea waste-immobilized Candida rugosa lipase 麻茶废料固定化白色念珠菌脂肪酶无溶剂酯化油酸的研究
IF 4.1 2区 生物学
Journal of biotechnology Pub Date : 2024-07-03 DOI: 10.1016/j.jbiotec.2024.07.001
Viviana Chiappini, Debora Casbarra, Maria Luisa Astolfi, Anna Maria Girelli
{"title":"Investigation on solvent-free esterification of oleic acid by hemp tea waste-immobilized Candida rugosa lipase","authors":"Viviana Chiappini,&nbsp;Debora Casbarra,&nbsp;Maria Luisa Astolfi,&nbsp;Anna Maria Girelli","doi":"10.1016/j.jbiotec.2024.07.001","DOIUrl":"10.1016/j.jbiotec.2024.07.001","url":null,"abstract":"<div><p>This study aimed at <em>Candida rugosa</em> lipase immobilization on a low-cost and readily available support. Among agro-industrial crops, hemp tea waste was chosen as the carrier because it provides higher immobilization performance than hemp flower and leaf wastes. Support characterization by ATR-FTIR, SEM and elemental analysis and the optimization of the adsorption immobilization process were performed. The lipase adsorption immobilization was obtained by soaking the support with hexane under mild agitation for 2 h and a successively incubating the enzyme for 1 h at room temperature without removing the solvent. The esterification of oleic acid with n-decanol was tested in a solvent-free system by studying some parameters that influence the reaction, such as the substrates molar ratio, the lipase activity/oleic acid ratio, reaction temperature and the presence/absence of molecular sieves. The biocatalyst showed the ability to bring the esterification reaction to equilibrium under 60 min and good reusability (maintaining 60 % of its original activity after three successive esterification reactions) but low conversion (21 %) at the optimized conditions (40 °C, 1:2 substrates molar ratio, 0.56 lipase/oleic acid ratio, without sieves). Comparing the results with those obtained by free lipase form at the same activity (1 U) and experimental conditions, slightly higher conversion (%) appeared for the free lipase. All this highlighted that probably the source of lipase for its carbohydrate-binding pocket and lid structure affected the esterification of oleic acid but certainly, the immobilization didn’t induce any lipase conformational change also allowing the reuse of the catalytic material.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"392 ","pages":"Pages 118-127"},"PeriodicalIF":4.1,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141537943","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Optimization of extended Kozak elements enhances recombinant proteins expression in CHO cells 优化扩展 Kozak 元件可提高 CHO 细胞中重组蛋白的表达。
IF 4.1 2区 生物学
Journal of biotechnology Pub Date : 2024-07-01 DOI: 10.1016/j.jbiotec.2024.06.020
Zheng-Mei Li , Yan Lin , Cong-hui Luo , Qiu-Li Sun , Chun-Liu Mi , Xiao-yin Wang , Tian-Yun Wang
{"title":"Optimization of extended Kozak elements enhances recombinant proteins expression in CHO cells","authors":"Zheng-Mei Li ,&nbsp;Yan Lin ,&nbsp;Cong-hui Luo ,&nbsp;Qiu-Li Sun ,&nbsp;Chun-Liu Mi ,&nbsp;Xiao-yin Wang ,&nbsp;Tian-Yun Wang","doi":"10.1016/j.jbiotec.2024.06.020","DOIUrl":"10.1016/j.jbiotec.2024.06.020","url":null,"abstract":"<div><p>In eukaryotes, the localization of small ribosomal subunits to mRNA transcripts requires the translation of Kozak elements at the starting site. The sequence of Kozak elements affects the translation efficiency of protein synthesis. However, whether the upstream nucleotide of Kozak sequence affects the expression of recombinant proteins in Chinese hamster ovary (CHO) cells remains unclear. In order to find the optimal sequence to enhance recombinant proteins expression in CHO cells, −10 to +4 sequences around ATG in 100 CHO genes were compared, and the extended Kozak elements with different translation intensities were constructed. Using the classic Kozak element as control, the effects of optimized extended Kozak elements on the secreted alkaline phosphatase (SEAP) and human serum albumin (HSA) gene were studied. The results showed that the optimized extended Kozak sequence can enhance the stable expression level of recombinant proteins in CHO cells. Furthermore, it was found that the increased expression level of the recombinant protein was not related with higher transcription level. In summary, optimizing extended Kozak elements can enhance the expression of recombinant proteins in CHO cells, which contributes to the construction of an efficient expression system for CHO cells.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"392 ","pages":"Pages 96-102"},"PeriodicalIF":4.1,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141498089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structure modeling-based characterization of ChnD, the 6-hydroxyhexanoate dehydrogenase from Acinetobacter sp. strain NCIMB 9871 基于结构建模的 ChnD(Acinetobacter sp. 菌株 NCIMB 9871 的 6-hydroxyhexanoate dehydrogenase)表征。
IF 4.1 2区 生物学
Journal of biotechnology Pub Date : 2024-06-29 DOI: 10.1016/j.jbiotec.2024.06.008
Ji-Min Woo , Hyun-Joo Kim , Se‑Yeun Hwang, Eun-Ji Seo, Jin-Byung Park
{"title":"Structure modeling-based characterization of ChnD, the 6-hydroxyhexanoate dehydrogenase from Acinetobacter sp. strain NCIMB 9871","authors":"Ji-Min Woo ,&nbsp;Hyun-Joo Kim ,&nbsp;Se‑Yeun Hwang,&nbsp;Eun-Ji Seo,&nbsp;Jin-Byung Park","doi":"10.1016/j.jbiotec.2024.06.008","DOIUrl":"10.1016/j.jbiotec.2024.06.008","url":null,"abstract":"<div><p>α,ω-Dicarboxylic acids, ω-aminoalkanoic acids, and α,ω-diaminoalkanes are valuable building blocks for the production of biopolyesters and biopolyamides. One of the key steps in producing these chemicals is the oxidation of ω-hydroxycarboxylic acids using alcohol dehydrogenases (e.g., ChnD of <em>Acinetobacter</em> sp. NCIMB 9871). However, the reaction and structural features of these enzymes remain mostly undiscovered. Thereby, we have investigated characteristics of ChnD based on enzyme kinetics, substrate-docking simulations, and mutation studies. Kinetic analysis revealed a distinct preference of ChnD for medium chain ω-hydroxycarboxylic acids, with the highest catalytic efficiency of 18.0 mM<sup>−1</sup>s<sup>−1</sup> for 12-hydroxydodecanoic acid among C6 to C12 ω-hydroxycarboxylic acids. The high catalytic efficiency was attributed to the positive interactions between the carboxyl group of the substrates and the guanidino group of two arginine residues (i.e., Arg62 and Arg266) in the substrate binding site. The ChnD_R62L variant showed the increased efficiency and affinity, particularly for fatty alcohols (i.e., C6–C10) and branched-chain fatty alcohols, such as 3-methyl-2-buten-1-ol. Overall, this study contributes to the deeper understanding of medium-chain primary aliphatic alcohol dehydrogenases and their applications for the production of industrially relevant chemicals such as α,ω-dicarboxylic acids, ω-aminoalkanoic acids, and α,ω-diaminoalkanes from renewable biomass.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"392 ","pages":"Pages 90-95"},"PeriodicalIF":4.1,"publicationDate":"2024-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141476645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Advancements in enzymatic biotransformation and bioactivities of rare ginsenosides: A review 稀有人参皂苷的酶生物转化和生物活性研究进展:综述。
IF 4.1 2区 生物学
Journal of biotechnology Pub Date : 2024-06-28 DOI: 10.1016/j.jbiotec.2024.06.018
Yanbo Hu , Yiming Li , Yi Cao , Yuzhu Shen , Xianjun Zou , Jiaxin Liu , Jun Zhao
{"title":"Advancements in enzymatic biotransformation and bioactivities of rare ginsenosides: A review","authors":"Yanbo Hu ,&nbsp;Yiming Li ,&nbsp;Yi Cao ,&nbsp;Yuzhu Shen ,&nbsp;Xianjun Zou ,&nbsp;Jiaxin Liu ,&nbsp;Jun Zhao","doi":"10.1016/j.jbiotec.2024.06.018","DOIUrl":"10.1016/j.jbiotec.2024.06.018","url":null,"abstract":"<div><p>Ginsenoside, the principal active constituent of ginseng, exhibits enhanced bioavailability and medicinal efficacy in rare ginsenosides compared to major ginsenosides. Current research is focused on efficiently and selectively removing sugar groups attached to the major ginsenoside sugar chains to convert them into rare ginsenosides that meet the demands of medical industry and functional foods. The methods for preparing rare ginsenosides encompass chemical, microbial, and enzymatic approaches. Among these, the enzyme conversion method is highly favored by researchers due to its exceptional specificity and robust efficiency. This review summarizes the biological activities of different rare ginsenosides, explores the various glycosidases used in the biotransformation of different major ginsenosides as substrates, and elucidates their respective corresponding biotransformation pathways. These findings will provide valuable references for the development, utilization, and industrial production of ginsenosides.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"392 ","pages":"Pages 78-89"},"PeriodicalIF":4.1,"publicationDate":"2024-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Non-thermal atmospheric-pressure plasma exposure as a practical method for improvement of Brassica juncea seed germination 将非热大气压等离子体暴露作为改善甘蓝种子萌发的实用方法。
IF 4.1 2区 生物学
Journal of biotechnology Pub Date : 2024-06-27 DOI: 10.1016/j.jbiotec.2024.06.019
Mime Kobayashi , Sho Yamaguchi , Shintaro Kusano , Shinya Kumagai , Toshiro Ito
{"title":"Non-thermal atmospheric-pressure plasma exposure as a practical method for improvement of Brassica juncea seed germination","authors":"Mime Kobayashi ,&nbsp;Sho Yamaguchi ,&nbsp;Shintaro Kusano ,&nbsp;Shinya Kumagai ,&nbsp;Toshiro Ito","doi":"10.1016/j.jbiotec.2024.06.019","DOIUrl":"10.1016/j.jbiotec.2024.06.019","url":null,"abstract":"<div><p>Here we report that non-thermal atmospheric-pressure plasma exposure can improve <em>Brassica juncea</em> (leaf mustard) seed germination rate from 50 % to 98 %. The commercially relevant germination rate was achieved by plasma exposure for only 10 minutes and the effect sustains at least for one month under an appropriate storage condition. Improved germination by plasma exposure was also observed for <em>Brassica rapa</em> subsp. <em>pekinensis</em> (Chinese cabbage) seeds. The plasma device used is simple. No pure gas flow system is necessary and it is easy to handle. A large number of seeds can be treated by simply scaling up the device. Plasma exposure can be a practical method for improving seed germination of crop plants important for agriculture.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"392 ","pages":"Pages 103-108"},"PeriodicalIF":4.1,"publicationDate":"2024-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S016816562400186X/pdfft?md5=55026990c00859cb30ddd829cb1dd8db&pid=1-s2.0-S016816562400186X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141468123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Biologically inspired nanoformulations for the control of bacterial canker pathogens Clavibacter michiganensis subsp. michiganensis and subsp. capsici 用于控制细菌性腐烂病病原体 Clavibacter michiganensis subsp.
IF 4.1 2区 生物学
Journal of biotechnology Pub Date : 2024-06-24 DOI: 10.1016/j.jbiotec.2024.06.017
Basma A. Omran , Muhammad Fazle Rabbee , Kwang-Hyun Baek
{"title":"Biologically inspired nanoformulations for the control of bacterial canker pathogens Clavibacter michiganensis subsp. michiganensis and subsp. capsici","authors":"Basma A. Omran ,&nbsp;Muhammad Fazle Rabbee ,&nbsp;Kwang-Hyun Baek","doi":"10.1016/j.jbiotec.2024.06.017","DOIUrl":"10.1016/j.jbiotec.2024.06.017","url":null,"abstract":"<div><p><em>Clavibacter michiganensis</em> subsp. <em>michiganensis</em> (<em>Cmm</em>) and <em>C. michiganensis</em> subsp. <em>capsici</em> (<em>Cmc</em>) are phytopathogenic bacteria that cause bacterial canker disease in tomatoes and peppers, respectively. Bacterial canker disease poses serious challenges to solanaceous crops, causing significant yield losses and economic costs. Effective management necessitates the development of sustainable control strategies employing nanobiotechnology. In this study, the antibacterial effects of four <em>Aspergillus sojae</em>-mediated nanoformulations, including cobalt oxide nanoparticles (Co<sub>3</sub>O<sub>4</sub> NPs), zinc oxide nanoparticles (ZnO NPs), cobalt ferrite nanoparticles (CoFe<sub>2</sub>O<sub>4</sub> NPs), and CoFe<sub>2</sub>O<sub>4</sub>/functionalized multi-walled carbon nanotube (fMWCNT) bionanocomposite, were evaluated against <em>Cmm</em> and <em>Cmc</em>. The diameters of the zone of inhibition of <em>A. sojae</em>-mediated Co<sub>3</sub>O<sub>4</sub> NPs, ZnO NPs, CoFe<sub>2</sub>O<sub>4</sub> NPs, and CoFe<sub>2</sub>O<sub>4</sub>/fMWCNT bionanocomposite against <em>Cmm</em> and <em>Cmc</em> were 23.60 mm, 22.09 mm, 27.65 mm, 22.51 mm, and 19.33 mm, 17.66 mm, 21.64 mm, 18.77 mm, respectively. The broth microdilution assay was conducted to determine the minimal inhibitory and bactericidal concentrations. The MICs of Co<sub>3</sub>O<sub>4</sub> NPs, ZnO NPs, CoFe<sub>2</sub>O<sub>4</sub> NPs, and CoFe<sub>2</sub>O<sub>4</sub>/fMWCNT bionanocomposite against <em>Cmm</em> were 2.50 mg/mL, 1.25 mg/mL, 2.50 mg/mL, and 2.50 mg/mL, respectively. While, their respective MBCs against <em>Cmm</em> were 5.00 mg/mL, 2.50 mg/mL, 5.00 mg/mL, and 5.00 mg/mL. The respective MICs of Co<sub>3</sub>O<sub>4</sub> NPs, ZnO NPs, CoFe<sub>2</sub>O<sub>4</sub> NPs, and CoFe<sub>2</sub>O<sub>4</sub>/fMWCNT bionanocomposite against <em>Cmc</em> were 2.50 mg/mL, 1.25 mg/mL, 5.00 mg/mL, and 5.00 mg/mL. While, their respective MBCs against <em>Cmc</em> were 5.00 mg/mL, 2.50 mg/mL, 10.00 mg/mL, and 10.00 mg/mL. The morphological and ultrastructural changes of <em>Cmm</em> and <em>Cmc</em> cells were observed using field-emission scanning and transmission electron microscopy before and after treatment with sub-minimal inhibitory concentrations of the nanoformulations. Nanoformulation-treated bacterial cells became deformed and disrupted, displaying pits, deep cavities, and groove-like structures. The cell membrane detached from the bacterial cell wall, electron-dense particles accumulated in the cytoplasm, cellular components disintegrated, and the cells were lysed. Direct physical interactions between the prepared nanoformulations with <em>Cmm</em> and <em>Cmc</em> cells might be the major mechanism for their antibacterial potency. Further research is required for the <em>in vivo</em> application of the mycosynthesized nanoformulations as countermeasures to combat bacterial phytopathogens.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"392 ","pages":"Pages 34-47"},"PeriodicalIF":4.1,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141457087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phytoconstituent-derived zingerone nanoparticles disrupt the cell adhesion mechanism and suppress cell motility in melanoma B16F10 cells 源于植物成分的姜酮纳米颗粒能破坏细胞粘附机制并抑制黑色素瘤 B16F10 细胞的移动。
IF 4.1 2区 生物学
Journal of biotechnology Pub Date : 2024-06-19 DOI: 10.1016/j.jbiotec.2024.06.015
Li-Wen Chu , Jun-Yih Chen , Yun-Wen Chen , Shuchen Hsieh , Mei-Lang Kung
{"title":"Phytoconstituent-derived zingerone nanoparticles disrupt the cell adhesion mechanism and suppress cell motility in melanoma B16F10 cells","authors":"Li-Wen Chu ,&nbsp;Jun-Yih Chen ,&nbsp;Yun-Wen Chen ,&nbsp;Shuchen Hsieh ,&nbsp;Mei-Lang Kung","doi":"10.1016/j.jbiotec.2024.06.015","DOIUrl":"10.1016/j.jbiotec.2024.06.015","url":null,"abstract":"<div><p>Combining phytochemicals and nanotechnology to improve the unfavorable innate properties of phytochemicals and develop them into potent nanomedicines to enhance antitumor efficacy has become a novel strategy for cancer chemoprevention. Melanoma is the most aggressive, metastatic, and deadly disease of the primary cutaneous neoplasms. In this study, we fabricated phytoconstituent-derived zingerone nanoparticles (NPs) and validated their effects on cell adhesion and motility in melanoma B16F10 cells. Our data indicated that zingerone NPs significantly induced cytotoxicity and anti-colony formation and inhibited cell migration and invasion. Moreover, zingerone NPs dramatically interfered with the cytoskeletal reorganization and markedly delayed the period of cell adhesion. Our results also revealed that zingerone NPs-mediated downregulation of MMPs (matrix metalloproteinases) activity is associated with inhibiting cell adhesion and motility. We further evaluated the effects of zingerone NPs on Src/FAK /Paxillin signaling, our data showed that zingerone NPs significantly inhibited the protein activities of Src, FAK, and Paxillin, indicating that they play important roles in zingerone NP-mediated anti-motility and anti-invasion in melanoma cells. Accordingly, the phytoconstituent-zingerone NPs can strengthen the inhibition of tumor growth, invasion, and metastasis in malignant melanoma. Altogether, these multi-pharmacological benefits of zingerone NPs will effectively achieve the purpose of melanoma prevention and invasion inhibition.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"392 ","pages":"Pages 48-58"},"PeriodicalIF":4.1,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141436964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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