Bioproducts from renewable methanol: The paraformaldehyde approach

IF 4.1 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jan A.M. de Bont , Bram J. Visscher , Timo J.P. van Roosmalen , Jan Wery , Bart W. Swinkels , Ger G. Bemer
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引用次数: 0

Abstract

Green methanol as feedstock in biotech operations at large scales is receiving in-depth attention for the production of Single Cell Protein (SCP) and circular chemicals. Several decades ago, cheap fossil-derived methanol was seen as an attractive feedstock. Now, renewable rather than fossil-derived methanol is in focus since its utilization does not depend on fossil resources and importantly it does not compete with food sources. Despite decade-long efforts, the biotech approaches have not been successful in generating economically viable large-scale production based on methanol. This impressive negative track record is to be attributed to the relatively reduced chemical nature of methanol, which implies excessive oxygen demands during fermentations. Hence, for large-scale methanol fermentations, it is essential to minimize oxygen budgets to arrive at economically-viable production processes. In this short communication, a new approach is described in reducing the oxygen footprint by employing the less-reduced compound paraformaldehyde which, via standard procedures, can be obtained chemically from methanol. It is a water-insoluble polymer, which slowly releases formaldehyde at ambient temperatures depending on either pH or temperature. Three methylotrophic microbes were demonstrated to metabolize formaldehyde as chemically released from the non-toxic paraformaldehyde. Methylophilus methylotrophus kept the monomeric formaldehyde below the detection limit in a stirred-tank bioreactor during a 20-hour run on paraformaldehyde. Based on the current work, it is concluded that paraformaldehyde is a highly-suitable feedstock for producing either SCP or numerous circular chemicals as derived from renewable methanol.
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来源期刊
Journal of biotechnology
Journal of biotechnology 工程技术-生物工程与应用微生物
CiteScore
8.90
自引率
2.40%
发文量
190
审稿时长
45 days
期刊介绍: The Journal of Biotechnology has an open access mirror journal, the Journal of Biotechnology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal provides a medium for the rapid publication of both full-length articles and short communications on novel and innovative aspects of biotechnology. The Journal will accept papers ranging from genetic or molecular biological positions to those covering biochemical, chemical or bioprocess engineering aspects as well as computer application of new software concepts, provided that in each case the material is directly relevant to biotechnological systems. Papers presenting information of a multidisciplinary nature that would not be suitable for publication in a journal devoted to a single discipline, are particularly welcome.
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