Journal of biotechnology最新文献

{"title":"A pump-free microfluidic co-culture system for investigating NK cell-tumor spheroid interactions in flow conditions.","authors":"Yuanyuan Xie, Ke Ning, Wen Sun, Lingke Feng, Yirong Chen, Wei Sun, Yan Li, Ling Yu","doi":"10.1016/j.jbiotec.2024.11.008","DOIUrl":"10.1016/j.jbiotec.2024.11.008","url":null,"abstract":"<p><p>Natural killer (NK) cells are pivotal in immunotherapy due to their potent tumor-targeting capabilities. However, accessible in vitro 3D dynamic models for evaluating Tumor Infiltrating Natural Killer Cells (TINKs) remain scarce. This study addresses this gap by developing a novel pump-free microfluidic chip to investigate the interactions between NK-92 cells and prostate DU 145 tumor spheroids. The platform facilitates the separation of free NKs and TINKs for subtype characterization. The design integrates multiple planes with a multi-layer paper scaffold to accommodate tumor spheroids, allowing NK-92 cells to traverse Matrigel-coated barriers that mimic the extracellular matrix. The dual-channel pump-free device enables unidirectional circulation of NK-92 cells, allowing analysis of tumor spheroid movement and NK-92 cell interactions under flow conditions. Results demonstrate continuous fluid circulation in the dual-channel device by rocking the platform at tilt angles of 21° and 15°. Tumor spheroids show- enhanced migration under flow conditions compared to static culture. Although spheroids recruit slightly more NK-92 cells under flow conditions, CD56 and CD16 receptor expression on IL-2-activated free NK-92 cells and tumor-infiltrating NK-92 cells matches in vivo patterns in dynamic cultures. These findings suggest that tumor cells and fluid dynamics significantly influence NK cell subtypes. This pump-free microfluidic platform is a functional tool for simulating and studying immune cell-tumor interactions, providing valuable insights into NK cell dynamics with tumor spheroids in physiologically relevant environments.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":"11-21"},"PeriodicalIF":4.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering Saccharomyces cerevisiae for continuous secretory production of hEGF in biofilm.","authors":"Kaiqi Zhi, Xiang Zhou, Tianping Gao, Kehan Liu, Zhenyu Wang, Yafan Cai, Zhi Wang, Shilei Wang, Jinle Liu, Dong Liu, Hanjie Ying","doi":"10.1016/j.jbiotec.2024.11.007","DOIUrl":"10.1016/j.jbiotec.2024.11.007","url":null,"abstract":"<p><p>Human epidermal growth factor (hEGF) plays a crucial role in promoting cell growth and has various clinical applications. Due to limited natural sources and the high cost of chemical synthesis, researchers are now exploring genetic engineering as a potential method for hEGF production. In this particular study, a novel hEGF expression system was developed using Saccharomyces cerevisiae. This system involved optimizing the promoter and signal peptide and deleting protease-coding genes PEP4, PRB1, and YAP3, overexpressing chaperones KAR2 and PDI1 in the protein secretion pathway, which led to a 2.01-fold increase in hEGF production compared to the wild type strain. Furthermore, biofilm-forming genes FLO11 and ALS3 were integrated to create a biofilm strain with adhesive properties. A biofilm-based immobilized continuous fermentation model was established to leverage the characteristics of this biofilm strain. Each batch of this model yielded 130 mg/L of hEGF, with a production efficiency of 2.71 mg/L/h - surpassing the production efficiency of traditional free fermentation (1.62 mg/L/h). This study presents a promising fermentation model for efficient hEGF production based on biofilm characteristics, offering valuable insights for the application of biofilm fermentation in the production of small molecule peptides.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":"1-10"},"PeriodicalIF":4.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142644207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digital holographic microscopy is suitable for lipid accumulation analysis in single cells of Yarrowia lipolytica.","authors":"Simon Carl-Philipp Briel, Nicolas Feuser, Eva Johanna Moldenhauer, Johannes Kabisch, Peter Neubauer, Stefan Junne","doi":"10.1016/j.jbiotec.2024.11.011","DOIUrl":"https://doi.org/10.1016/j.jbiotec.2024.11.011","url":null,"abstract":"<p><p>Digital holographic microscopy (DHM) is a label-free analytical technique for the determination of the cells' volume and their cytosolic refractive index. Here, we demonstrate the suitability of DHM for the quantification of total lipid accumulation in the oleaginous yeast Yarrowia lipolytica. Presently, microbial lipids are gaining increasing attention due to their nutritional value in feed and food applications. Their microbiological synthesis in algae and yeast is subject to optimization studies, which necessitates rapid quantification of total lipids for faster progress and the possibility of process control. So far, quantification of the total intracellular long-chain fatty acid concentration in yeast cells is time-consuming though when common chromatography for a volumetric analysis or staining and flow cytometry for a single-cell based analysis are used. This study, however, demonstrates that 3D-DHM facilitates a quasi-real-time measurement that allows for a rapid quantification of total intracellular lipid accumulation on a single-cell level without cell staining. Data from wild-type and lipidoverproducing Y. lipolytica strains with specific yields of long-chain fatty acids in a range between 70 to 360mg/gCDW show a good correlation with the optical volume determined by DHM, as the total lipid accumulation in the cell is typically wellcorrelated with the long-chain fatty acid concentration. The results further correlate with data obtained from gas chromatography and flow cytometry of Nile Red-stained cells, which proves the reliability of DHM for lipid quantification in Y. lipolytica.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142648166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydrogen production capabilities of lichens micro-ecosystem under extreme salinity, crystalline salt exposure, and simulated Mars-like conditions","authors":"Maria Fanara ,&nbsp;Aikaterini Papazi ,&nbsp;Stergios Pirintsos ,&nbsp;Kiriakos Kotzabasis","doi":"10.1016/j.jbiotec.2024.11.004","DOIUrl":"10.1016/j.jbiotec.2024.11.004","url":null,"abstract":"<div><div>This work aims to demonstrate the extremophilic behavior of the lichen <em>Pleurosticta acetabulum</em> at extreme salinities, while maintaining its metabolic capacity to produce hydrogen. Lichen is a special micro-ecosystem that includes mostly a fungus and a green alga or cyanobacterium, as well as a microbiome. The peculiarity of this symbiotic system is its ability to dry out completely and stay inactive to survive harsh conditions. Lichens that had been dehydrated for six months revived quickly when rehydrated, restoring their photosynthetic efficiency and ability to produce hydrogen. The lichen microbiome was crucial for hydrogen production, especially through dark fermentation. The experiments of this work showed that lichen during its exposure to different salinity conditions (0 %NaCl – control, 3,5 %NaCl – sea salt concentration, 36 %NaCl – saturated salt concentration), but also after exposure to crystalline salt (100 %NaCl) could maintain the structure and the functionality of its photosynthetic apparatus. This was tested using chlorophyll a fluorescence induction measurements. Based on the results from gas chromatography with thermal conductivity detection (GC-TCD) used to determine hydrogen production, it was shown that despite being exposed to extreme salinity conditions, lichens maintained their ability to produce hydrogen. The experimental combination of lichen exposure to extreme salinities (up to 100 % NaCl), with an extreme atmosphere (100 % CO₂) and low atmospheric pressure (&lt;10mbar), simulating Mars conditions, highlighted the functional potential of the lichen for survival in a Mars-like environment. This lichen’s ability to withstand extreme conditions and to produce large amounts of hydrogen, makes it a promising candidate for future biotechnological applications, even in challenging environments like Mars, opening new astrobiological and astrobiotechnological perspectives.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 171-179"},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structure-guided engineering of 4-coumarate: CoA ligase for efficient production of rosmarinic acid in Saccharomyces cerevisiae","authors":"Xiuqi Zhou ,&nbsp;Jiayan Du ,&nbsp;Jinyuan Zhu ,&nbsp;Xueqing Pang ,&nbsp;Xinjian Yin ,&nbsp;Pingping Zhou","doi":"10.1016/j.jbiotec.2024.11.006","DOIUrl":"10.1016/j.jbiotec.2024.11.006","url":null,"abstract":"<div><div>The utilization of genetically modified microbial cells for rosmarinic acid (RA) production is gaining increased attention as a cost-effective and sustainable approach. However, the substrate promiscuity of 4-coumarate: CoA ligase and RA synthase has been considered as a critical factor for low RA yields. In this study, we rationally engineered the substrate preference of 4-coumarate: CoA ligase (OPc4CL2) from <em>Petroselinum crispum</em>, resulting in a significant enhancement in RA production. Particularly, the introduction of the Y240C mutation led to a remarkable 176 % increase in RA yield. Subsequent enzymatic analysis of OPc4CL2 variants revealed diminished activity towards <em>p</em>-coumaric acid, resulting in insufficient time for the transformation of <em>p</em>-coumaric acid to 4-coumaroyl CoA to generate byproduct. Furthermore, to minimize the formation of undesired byproducts, the overexpression of 4-hydroxyphenylacetate 3-monooxygenase (OHpaB) and NADPH-flavin oxidoreductase (HpaC) was carried out to facilitate the conversion of <em>p</em>-coumaric acid to caffeic acid and 4-hydroxyphenyllactate to salvianic acid A, thus achieving a significant increase in RA yield of up to 329.9 mg/L (16.5 mg/g yield on glucose) in shake-flask cultivation. Finally, the engineered strain YRA113–24BHM achieved a notable RA production of 3.6 g/L (about 20.2 mg/g yield on glucose) by fed-batch fermentation. This study serves as a foundation for the sustainable biosynthesis of RA and other caffeic acid derivatives.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 140-149"},"PeriodicalIF":4.1,"publicationDate":"2024-11-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621085","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimisation of coumaric acid production from aromatic amino acids in Kluyveromyces marxianus","authors":"Joel A. Akinola ,&nbsp;Arun S. Rajkumar ,&nbsp;John P. Morrissey","doi":"10.1016/j.jbiotec.2024.11.002","DOIUrl":"10.1016/j.jbiotec.2024.11.002","url":null,"abstract":"<div><div>Yeasts are attractive hosts for the production of heterologous products due to their genetic tractability and relative ease of growth. While the baker’s yeast <em>Saccharomyces cerevisiae</em> is a powerful workhorse of the biotechnology industry, the species has metabolic limitations and it is critical that we develop alternative platforms that will facilitate the development of bioprocesses that rely on sustainable feedstocks. In this study, we used synthetic biology tools to construct coumaric acid–producing strains of <em>Kluyveromyces marxianus,</em> a yeast whose physiological traits render it attractive for biotechnology applications. Coumaric acid is a building block in the synthesis of many different families of aromatics and is a key precursor for the synthesis of complect phenylpropanoid molecules, including many flavours and aromas. The starting point for this work was a <em>K. marxianus</em> chassis strain that has increased flux towards the synthesis of tyrosine and phenylalanine, the aromatic amino acids that can serve as starting points for coumaric acid synthesis. Following principles of synthetic biology, a modular approach was taken to identify the best solution to different metabolic possibilities and these were then combined in different ways. For the first step, it was established that the route from phenylalanine was superior to that from tyrosine and the combined overexpression of <em>PlPAL</em>, <em>AtC4H</em> and <em>AtCPR1</em> delivered the highest yield of coumaric acid. Next, it was established that while Pdc5 and Aro10 both had phenylpyruvate decarboxylase activity, inactivation of <em>ARO10</em> was sufficient to prevent flux loss in the pathway. Since phenylalanine is the starting point, efforts were made to improve efficiency of its production. It was found that glutamate was a preferred nitrogen source for coumaric acid production, and this knowledge was used to engineer a strain that overexpressed <em>S. cerevisiae GDH1</em> and delivered higher yields of coumaric acid. Ultimately, this strategy led to the development of strains that has yields of up to 48 mg coumaric acid /g glucose. Strains were evaluated in bioreactors to investigate the effects of different process parameters. These analyses indicated that engineered strains face some redox balance challenges and further work will be required overcome these to develop strains that can perform well under industrial conditions.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 158-170"},"PeriodicalIF":4.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Markerless deletion of the putative type I and III restriction-modification systems in the cellulolytic bacterium Clostridium cellulovorans using a codBA-based counterselection technique.","authors":"Luciana Almeida, Aline Schöllkopf, Holger Edelmann, Armin Ehrenreich, Wolfgang Liebl","doi":"10.1016/j.jbiotec.2024.11.001","DOIUrl":"10.1016/j.jbiotec.2024.11.001","url":null,"abstract":"<p><p>Cellulose from lignocellulosic biomass (LB) is of increasing interest for the production of commodity chemicals. However, its use as substrate for fermentations is a challenge due to its structural complexity. In this context, the highly cellulolytic Clostridium cellulovorans has been considered an interesting microorganism for the breakdown of LB. C. cellulovorans does not naturally produce solvents in useful concentrations, but this could be achieved by metabolic engineering. Unfortunately, this is hampered by the lack of tools for genetic engineering. We describe a genetic system that allows strain engineering by the allelic-coupled exchange method. First, the Gram-positive origin of pUB110 was identified as a suitable clostridial 'pseudo-suicide' origin of replication for the construction of deletion vectors. Second, an efficient counterselection strategy based on a codBA cassette and the use of 5-fluorocytosine as the counterselective compound was employed. Third, since the prevention of DNA transfer by host restriction-modification (RM) systems is a critical barrier to genome engineering, deletion plasmids containing flanking regions for the putative type I (Clocel_1114) and III (Clocel_2651) RM systems were constructed and transferred into C. cellulovorans. The restriction-less strains C. cellulovorans ΔClocel_1114 and C. cellulovorans ΔClocel_2651 exhibit high conjugation efficiency and can be easily used for further metabolic engineering.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":"22-31"},"PeriodicalIF":4.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanding the high-pH range of the sucrose synthase reaction by enzyme immobilization","authors":"Hui Liu ,&nbsp;Annika J.E. Borg ,&nbsp;Bernd Nidetzky","doi":"10.1016/j.jbiotec.2024.11.005","DOIUrl":"10.1016/j.jbiotec.2024.11.005","url":null,"abstract":"<div><div>The glycosylation of an alcohol group from a sugar nucleotide substrate involves proton release, so the reaction is favored thermodynamically at high pH. Here, we explored expansion of the alkaline pH range of sucrose synthase (SuSy; EC 2.4.1.13) to facilitate enzymatic glycosylation from uridine 5’-diphosphate (UDP)-glucose. The apparent equilibrium constant of the SuSy reaction (UDP-glucose + fructose ↔ sucrose + UDP) at 30 °C increases by ∼4 orders of magnitude as the pH is raised from 5.5 to 9.0. However, the SuSy in solution loses ≥80 % of its maximum productivity at pH ∼7 when alkaline reaction conditions (pH 9.0) are used. We therefore immobilized the SuSy on nanocellulose-based biocomposite carriers (∼48 U/g carrier; ≥ 50 % effectiveness) and reveal in the carrier-bound enzyme a substantial broadening of the pH-productivity profile to high pH, with up to 80 % of maximum capacity retained at pH 9.5. Using reaction by the immobilized SuSy with automated pH control at pH ∼9.0, we demonstrate near-complete conversion (≥ 96 %) of UDP-glucose and fructose (each 100 mM) into sucrose, as expected from the equilibrium constant (<em>K</em>_eq = ∼7 × 10²) under these conditions. Collectively, our results support the idea of glycosyltransferase-catalyzed synthetic glycosylation from sugar nucleotide donor driven by high pH; and they showcase a marked adaptation to high pH of the operational activity of the soybean SuSy by immobilization.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 150-157"},"PeriodicalIF":4.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient biosynthesis of three rare formononetin derivatives by newly discovered Bacillus velezensis LQ5","authors":"Jiaqi Liu ,&nbsp;Fan Yang ,&nbsp;Wenxin Ji ,&nbsp;Lin Zhao ,&nbsp;Jing Han ,&nbsp;Liangliang Chen ,&nbsp;Fucheng Zhu ,&nbsp;Jinao Duan ,&nbsp;Sen Zhang","doi":"10.1016/j.jbiotec.2024.11.003","DOIUrl":"10.1016/j.jbiotec.2024.11.003","url":null,"abstract":"<div><div>Formononetin is a natural flavonoid existing widely in plants with many pharmacological effects. However, its application is limited by structure, poor water solubility and low bioavailability. In this study, <em>Bacillus velezensis</em> LQ5 was isolated from the inter-root soil of <em>Glycyrrhiza uralensis</em> Fisch for the first time and formononetin was firstly structurally modified by whole-cell catalysis of LQ5 to obtain formononetin-7-O-β-D-glucoside (FG), formononetin-7-O-β-(6''-O-succinyl)-D-glucoside (FGS) and formononetin-7-O-phosphate (FP). The selective preparation of the three products was achieved by adjusting the content of yeast extract, type and content of sugars, metal ions, pH and ATP content. The result confirmed that FP had the ideal drug-likeness properties and showed a greater ability to reduce intracellular reactive oxygen species levels and regulate oxidative enzymes. This work successfully established a biotransformation method for the efficient transformation of formononetin to produce high-value formononetin derivatives.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 116-126"},"PeriodicalIF":4.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detrimental effects of UV-A radiation on antioxidant capacity and photosynthetic efficiency on a tropical microalga","authors":"Anna Isaia ,&nbsp;Noémie Coulombier ,&nbsp;Loïc Le Dean ,&nbsp;Vincent Mériot ,&nbsp;Thierry Jauffrais","doi":"10.1016/j.jbiotec.2024.10.013","DOIUrl":"10.1016/j.jbiotec.2024.10.013","url":null,"abstract":"<div><div>Antioxidants are molecules able to neutralize reactive oxygen species with potential applications in the cosmetic or nutraceutical industries. Abiotic stressors, such as light intensity, ultraviolet (UV) radiation, or nutrient availability, can influence their production. In the perspective of optimizing and understanding the antioxidant capacity of microalgae, we investigated the effects of UV-A radiation on growth, and antioxidant and photosynthetic activities on <em>Tetraselmis</em>, a microalga genus known for its high antioxidant capacity. Cultures were exposed to UV-A radiation alongside to photosynthetically active radiation (PAR) in photobioreactors operated in continuous culture. UV-A exposure affects both the photosynthetic and antioxidant activities of <em>Tetraselmis</em>. Photosynthetic parameters suggest that UV-A has a negative effect on photosynthetic efficiency, particularly on the electron transport chain on short-term exposure (1–2 days). However, a resilience of most physiological parameters was observed over the experiment (10 days) suggesting a photochemical adaption over long-term exposure to UV-A radiation. Concerning the antioxidant capacity, UV-A exposure reduced the antioxidant capacity in <em>Tetraselmis</em> suggesting the use of antioxidant molecules to counteract reactive oxygen species production and prevent damage to photosystem II. Finally, the highest antioxidant capacity never observed with a <em>Tetraselmis</em> sp. was measured in cultures without UV addition, with an IC₅₀ of 2.87 ± 0.24 µg mL⁻¹, a value close to the reference compounds Trolox and α-tocopherol. This study showed the great potential of <em>Tetraselmis</em> as a source of antioxidants under favorable culture condition and without UV-A radiations. Indeed, we discourage the use of UV-A to enhance antioxidant capacity in this species due to its negative impact on it and on the photosynthetic efficiency.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 104-115"},"PeriodicalIF":4.1,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}