Journal of biotechnology最新文献

Establishment of high-efficiency hairy root and genetic transformation system in Cynanchum stauntonii.

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-03-08 DOI: 10.1016/j.jbiotec.2025.02.006

Jingyi Zhang, Xingfang Mao, Bisheng Huang, Mi Lei, Yuhuan Miao, Dahui Liu

{"title":"Establishment of high-efficiency hairy root and genetic transformation system in Cynanchum stauntonii.","authors":"Jingyi Zhang, Xingfang Mao, Bisheng Huang, Mi Lei, Yuhuan Miao, Dahui Liu","doi":"10.1016/j.jbiotec.2025.02.006","DOIUrl":"https://doi.org/10.1016/j.jbiotec.2025.02.006","url":null,"abstract":"<p><p>Cynanchum stauntonii is an important medicinal plant with antitussive effect, and its roots and rhizomes are the main medicinal part. The saponins of C. stauntonii are generally considered to be the active ingredients. The hairy root (HR) system is an important production system for secondary metabolites. However, the systems for HR induction and transformation of C. stauntonii have not been reported. Using Agrobacterium rhizogenes A4 and 20-day young stems, we induced HRs in C. stauntonii with a 79.5% success rate. The results showed that the content of steroid alkaloids, such as cortisone, tomatidine, jurubine and 18-hydroxycorticosterone in HRs, were increased compared with normal roots (NRs). We applied three elicitors-chitosan, turpentine, and MeJA to enhance the biosynthesis of active components in HRs. The results showed that 10mg/L chitosan could increase the content of active ingredients in HRs by over two-fold. Additionally, we successfully established a genetic hairy root transformation system with a 56.36% success rate, enabling betalain production up to 1.24mg/g in dry weight of HRs. This research is the first to report HR induction and transformation systems for C. stauntonii, laying a crucial foundation for future functional genomics studies and the production of active components in this valuable medicinal plant.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioproducts from renewable methanol: The paraformaldehyde approach

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-03-07 DOI: 10.1016/j.jbiotec.2025.03.004

Jan A.M. de Bont , Bram J. Visscher , Timo J.P. van Roosmalen , Jan Wery , Bart W. Swinkels , Ger G. Bemer

{"title":"Bioproducts from renewable methanol: The paraformaldehyde approach","authors":"Jan A.M. de Bont , Bram J. Visscher , Timo J.P. van Roosmalen , Jan Wery , Bart W. Swinkels , Ger G. Bemer","doi":"10.1016/j.jbiotec.2025.03.004","DOIUrl":"10.1016/j.jbiotec.2025.03.004","url":null,"abstract":"<div><div>Green methanol as feedstock in biotech operations at large scales is receiving in-depth attention for the production of Single Cell Protein (SCP) and circular chemicals. Several decades ago, cheap fossil-derived methanol was seen as an attractive feedstock. Now, renewable rather than fossil-derived methanol is in focus since its utilization does not depend on fossil resources and importantly it does not compete with food sources. Despite decade-long efforts, the biotech approaches have not been successful in generating economically viable large-scale production based on methanol. This impressive negative track record is to be attributed to the relatively reduced chemical nature of methanol, which implies excessive oxygen demands during fermentations. Hence, for large-scale methanol fermentations, it is essential to minimize oxygen budgets to arrive at economically-viable production processes. In this short communication, a new approach is described in reducing the oxygen footprint by employing the less-reduced compound paraformaldehyde which, via standard procedures, can be obtained chemically from methanol. It is a water-insoluble polymer, which slowly releases formaldehyde at ambient temperatures depending on either pH or temperature. Three methylotrophic microbes were demonstrated to metabolize formaldehyde as chemically released from the non-toxic paraformaldehyde. <em>Methylophilus methylotrophus</em> kept the monomeric formaldehyde below the detection limit in a stirred-tank bioreactor during a 20-hour run on paraformaldehyde. Based on the current work, it is concluded that paraformaldehyde is a highly-suitable feedstock for producing either SCP or numerous circular chemicals as derived from renewable methanol.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"402 ","pages":"Pages 1-4"},"PeriodicalIF":4.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585842","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Strengthening core-region hydrogen-bond networks and rigidifying surface loop to enhance thermostability of an (R)-selective transaminase converting chiral hydroxyl amines.

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-03-07 DOI: 10.1016/j.jbiotec.2025.03.006

Yuwen Wei, Fulong Li, Yukun Zheng, Youxiang Liang, Yan Du, Huimin Yu

{"title":"Strengthening core-region hydrogen-bond networks and rigidifying surface loop to enhance thermostability of an (R)-selective transaminase converting chiral hydroxyl amines.","authors":"Yuwen Wei, Fulong Li, Yukun Zheng, Youxiang Liang, Yan Du, Huimin Yu","doi":"10.1016/j.jbiotec.2025.03.006","DOIUrl":"https://doi.org/10.1016/j.jbiotec.2025.03.006","url":null,"abstract":"<p><p>Transaminases have important applications in the synthesis of drug intermediates such as chiral amines. However, natural transaminases exhibit suboptimal thermal stability, limiting their further applications. Building upon an Rhodobacter sp.-derived (R)-selective transaminase (RbTA), we report a dual-region coupling engineering approach to improve thermostability of RbTA by strengthening the core hydrogen-bond networks and rigidifying the flexible surface loop. Through single strategy, we identified 4 thermostability improved single mutations, among which I249Q demonstrated the most substantial improvement, achieving a 18-fold increase in half-life (t<sub>1/2</sub><sup>40</sup>) and a 11.2 ℃ increase in T<sub>50</sub><sup>10</sup>. Then in strategic coupling, the synergistic effect of dual-region modification was observed in both thermal stability and activity enhancement, as mutant with the best high-temperature catalytic performance, R136P/F228Y, had its T<sub>50</sub><sup>10</sup> improved by 7.1℃ and exhibited a 4.2-fold increase in k<sub>cat</sub>/K<sub>m</sub> towards (R)-3-amino-1-butanol. Finally, R136P/F228Y achieved a 20.5% improvement in conversion over WT in an analytical-scale synthesis in 72h at a 5 ℃ elevated catalytic temperature. Molecular dynamics simulations demonstrated that the synergy of the formation of new hydrogen bonds and decrease in flexibility accounted for the thermostability improvements. This study provides guidance for enhancing thermostability of similar fold-type enzymes without impairing enzymatic activity in an efficient manner.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143585843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The promoter of Zymomonas mobilis respiratory NADH dehydrogenase (ndh) is induced by oxygen

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-03-07 DOI: 10.1016/j.jbiotec.2025.03.005

Marta Rubina , Inese Strazdina , Reinis Rutkis, Uldis Kalnenieks

引用次数: 0

Trypsin-catalyzed asymmetric aldol reactions of isatins with cyclic ketones and the mechanistic insights on activity differences at theoretical level.

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-03-06 DOI: 10.1016/j.jbiotec.2025.03.001

Cheng Fu, Xinying Wang, Zhuoyi Liu, Yulong Li, Yaping Zhu, Wei Zhang

引用次数: 0

Identification and Characterization of Anaerobically Activated Promoters in Escherichia coli.

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-03-04 DOI: 10.1016/j.jbiotec.2025.03.002

Sen Yang, Chao-Hao Guo, Wen-Yue Tong, Xiao-Yun Liu, Jing-Chen Li, Ming Kang

{"title":"Identification and Characterization of Anaerobically Activated Promoters in Escherichia coli.","authors":"Sen Yang, Chao-Hao Guo, Wen-Yue Tong, Xiao-Yun Liu, Jing-Chen Li, Ming Kang","doi":"10.1016/j.jbiotec.2025.03.002","DOIUrl":"https://doi.org/10.1016/j.jbiotec.2025.03.002","url":null,"abstract":"<p><p>Anaerobically activated promoters in Escherichia coli play crucial roles in transcriptional regulation during cellular responses to decreased oxygen concentrations and serve as essential tools for implementing dynamic regulation in metabolic engineering. These promoters exhibit transcriptional activity only under low-oxygen or anaerobic conditions. To discover novel anaerobically activated promoters, this study selected 11 native promoters from E. coli databases and characterized their activities using flow cytometry. Subsequently, we optimized the key elements of these promoters and re-evaluated their activities to investigate the impact of functional elements on promoter performance. Furthermore, we verified the regulatory mechanisms of these promoters by knocking out host regulatory genes. Finally, we characterized the promoters' responsiveness to aerobic-anaerobic transitions by rapidly switching cultivation environments during host growth. This study identified several novel anaerobically activated promoters and comprehensively characterized their performance and features from multiple aspects. The identified promoters provide new tools for oxygen-limited or anaerobic production in metabolic engineering, while the findings from promoter element optimization offer valuable references for the design of anaerobically activated promoters.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Long-term bioreactor cultivation affects dioscin content and the ratio of 25(S)- and 25(R)-protodioscin isomers in the suspension cell culture of Dioscorea deltoidea Wall.

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-03-04 DOI: 10.1016/j.jbiotec.2025.02.012

Maria V Titova, Elena V Popova, Igor M Ivanov, Olga N Prudnikova, Tatiana M Tyurina, Pavel S Metalnikov, Nadezhda V Kupaeva, Andrey B Lisitsyn, Boris A Sarvin, Igor A Rodin, Andrey N Stavrianidi

{"title":"Long-term bioreactor cultivation affects dioscin content and the ratio of 25(S)- and 25(R)-protodioscin isomers in the suspension cell culture of Dioscorea deltoidea Wall.","authors":"Maria V Titova, Elena V Popova, Igor M Ivanov, Olga N Prudnikova, Tatiana M Tyurina, Pavel S Metalnikov, Nadezhda V Kupaeva, Andrey B Lisitsyn, Boris A Sarvin, Igor A Rodin, Andrey N Stavrianidi","doi":"10.1016/j.jbiotec.2025.02.012","DOIUrl":"https://doi.org/10.1016/j.jbiotec.2025.02.012","url":null,"abstract":"<p><p>Bioreactor-grown plant cells have emerged as a sustainable, high-quality source of plant biomass and bioactive phytochemicals alternative to overcollection of pharmaceutically important wild plant species. At the same time, concerns were raised about the potential biosynthetic instability of plant cell cultures during long-term bioreactor cultivation, which was rarely investigated. In this work, this concern was addressed by performing the first long-term (1.5 years) uninterrupted cultivation of Dioscorea deltoidea cell suspension in a 20-L bubble-type bioreactor using fill-and-draw mode with simultaneous monitoring of major bioactive compounds - steroidal glycosides protodioscin and dioscin, using HPLC-ESI-MS. In addition, the ratio of 25(S)/25(R)-isomers of protodioscin showing different pharmacological activities was monitored during the entire cultivation period. The results demonstrated that cell culture productivity (0.33g/(L·day)), maximum dry weight accumulation (8.5g/L), viability (80.2%), and the total content of steroidal glycosides (1.74% of dry weight) remained high during the entire cultivation. However, the content of dioscin, a spirostanol steroidal glycoside, decreased by 82% after one year of cultivation. Moreover, the ratio of 25(S)/25(R)-isomers of protodioscin, a furostanol steroidal glycoside, in the cell biomass changed reversely from 0.66 to 1.40 after the first half-year of the cultivation. These results evidenced the complex dynamics of steroidal glycosides biosynthesis in plant cell cultures during the prolonged bioreactor cultivation and advocate for the importance of monitoring both the concentration and the isomeric composition of the desired metabolites to assure high quality of the biotechnologically produced cell biomass.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143572914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimization of fungal fermentation for the extraction of polyphenols from Flourensia cernua and its effect on cellular metabolism

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-21 DOI: 10.1016/j.jbiotec.2025.02.011

Lesly Katleya Usme-Duque , Jesús A. Claudio-Rizo , José Alberto Nuncio-Esquivel , María I. León-Campos , Marisol Cruz-Requena , Leopoldo J. Ríos-González , Juan A. Ascacio- Valdés , Miguel A. Medina-Morales

{"title":"Optimization of fungal fermentation for the extraction of polyphenols from Flourensia cernua and its effect on cellular metabolism","authors":"Lesly Katleya Usme-Duque , Jesús A. Claudio-Rizo , José Alberto Nuncio-Esquivel , María I. León-Campos , Marisol Cruz-Requena , Leopoldo J. Ríos-González , Juan A. Ascacio- Valdés , Miguel A. Medina-Morales","doi":"10.1016/j.jbiotec.2025.02.011","DOIUrl":"10.1016/j.jbiotec.2025.02.011","url":null,"abstract":"<div><div>This study aimed to maximize the potential of <em>Flourensia cernua</em> as a source of phenolic compounds through solid-state fermentation with <em>Aspergillus niger</em>, focusing on evaluating the antioxidant activity of the extracted compounds and their effects on modulating the metabolism of animal, cancerous, and plant cells. Initially, a comparison of the phytochemical profiles between macerated and fermented extracts was conducted. Different culture conditions were then assessed using a Plackett-Burman design (including salt concentration, inoculum concentration, moisture, and pH) to identify the most significant factors. This was followed by a response surface methodology to optimize the concentration of hydrolyzable phenolic compounds. Moisture and KH₂PO₄ concentration were identified as critical parameters for enhancing phenolic content, resulting in a final concentration of 43.440 mg GAE/g. The chemical composition of these extracts was analyzed using infrared spectroscopy and X-ray diffraction, confirming the presence of characteristic polyphenol functional groups, along with inorganic compounds such as MgO, SiO₂, and CaO. <em>In vitro</em> metabolic evaluations of animal and plant cells exposed to the extracts revealed a marked stimulation of 3T3 fibroblast and bone cell metabolism with the fermented extract. Moreover, the phenolic compounds in the extract exhibited cytotoxic effects on HeLa and colon cancer cells at 48 hours. Regarding plant cells derived from red and green tomato, cantaloupe, and watermelon seeds, the fermented extract significantly stimulated metabolic activity after 48 hours of exposure. These findings suggest that fermented extracts of <em>Flourensia cernua</em> with <em>Aspergillus niger</em> hold promise for various biotechnological applications.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"401 ","pages":"Pages 60-73"},"PeriodicalIF":4.1,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficiently scaled-up production of recombinant human elastin-like polypeptides using multiple optimization strategies

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-20 DOI: 10.1016/j.jbiotec.2025.02.010

Jianwei Xiong , Longyin Liu , Wei Yu , Min Li , Luping Zhou , Longhua Dai , Nuoyi Ning , Xinmiao Liang , Xianlong Ye

{"title":"Efficiently scaled-up production of recombinant human elastin-like polypeptides using multiple optimization strategies","authors":"Jianwei Xiong , Longyin Liu , Wei Yu , Min Li , Luping Zhou , Longhua Dai , Nuoyi Ning , Xinmiao Liang , Xianlong Ye","doi":"10.1016/j.jbiotec.2025.02.010","DOIUrl":"10.1016/j.jbiotec.2025.02.010","url":null,"abstract":"<div><div>Elastin-like polypeptides (ELPs) are biopolymers with repetitive amino acid sequences and are known for their biocompatibility and inverse transition cycling (ITC) properties; thus, they are ideal for biomedical applications. Owing to their low yield and tedious purification process with multiple rounds of ITC, no acceptable scaled-up production process has been developed. Here, for the first time, an efficient, low-cost process for the preparation of recombinant human elastin-like polypeptide (rhELP) is reported. This process leverages high-cell-density fermentation and hollow fibre membrane (HFM) filtration technology. First, we constructed an engineered strain of <em>Escherichia coli</em> (<em>E. coli</em>) with high expression of the rhELP protein, and a yield of 0.99 ± 0.03 g/L was achieved in shaker flasks by optimizing the induction temperature, induction OD<sub>600</sub>, and inducer concentration via response surface methodology. Further optimization in 5 L, 200 L, and 500 L automated fermenters increased the yield to over 5.00 g/L, which meets the demands of industrial production. The efficient purification process included high-pressure homogenization, flocculation, salting out, HFM filtration, ion-exchange chromatography (IEC), and ultrafiltration and resulted in 99.83 % pure rhELP analyzed by size exclusion–high-performance liquid chromatography (SEC-HPLC), with a recovery rate of 80.40 %. The prepared protein was noncytotoxic and exhibited marked wound healing promotion both <em>in vivo</em> and <em>in vitro</em>. Thus, this study provides a universal paradigm for the industrial production of ELPs and other similar recombinant proteins, significantly advancing the commercialization of promising ELPs.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"401 ","pages":"Pages 32-47"},"PeriodicalIF":4.1,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143474210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dark septate endophytic fungus Alternaria sp. 17463 regulates various antioxidant enzymes and compounds to mitigate salt stress caused by different anion salts. 暗隔内生真菌 Alternaria sp. 17463 可调节各种抗氧化酶和化合物，以减轻不同阴离子盐引起的盐胁迫。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-20 DOI: 10.1016/j.jbiotec.2025.02.008

Shishuang Zhang , Yinli Bi , Hai Tan

{"title":"Dark septate endophytic fungus Alternaria sp. 17463 regulates various antioxidant enzymes and compounds to mitigate salt stress caused by different anion salts.","authors":"Shishuang Zhang , Yinli Bi , Hai Tan","doi":"10.1016/j.jbiotec.2025.02.008","DOIUrl":"10.1016/j.jbiotec.2025.02.008","url":null,"abstract":"<div><div>In the field of agricultural and environmental management, understanding how dark septate endophytic fungi (DSE) like <em>Alternaria</em> sp. 17463 respond to salt stress is crucial. Prior research has yet to fully explore the anion-specific responses of DSE to salt stress. In this study, we delve into the physiological and biochemical responses of <em>Alternaria</em> sp. 17463 under NaCl and Na<sub>2</sub>SO<sub>4</sub> stress, employing a suite of analytical techniques. We discovered a marked disparity in fungal tolerance, with NaCl inducing a 50 % growth reduction at 0.6 M and a complete growth arrest at 1.4 M, contrasting with Na<sub>2</sub>SO<sub>4</sub>'s milder 30 % impact at the highest tested concentration. Cell membrane integrity was severely compromised under NaCl, with a 70 % increase in permeability and a 40 % plummet in cell viability at 1.4 M, whereas Na<sub>2</sub>SO<sub>4</sub> induced only a 20 % permeability increase. Antioxidant enzyme profiling revealed a twofold surge in superoxide dismutase (SOD) activity under NaCl at 0.4 M, and a 1.5-fold rise in catalase (CAT) activity under Na<sub>2</sub>SO<sub>4</sub>. Furthermore, there was a significant correlation between Na<sup>+</sup> concentration and cellular responses, particularly under Na<sub>2</sub>SO<sub>4</sub> stress, where higher sodium tolerance was linked to enhanced melanin, reduced glutathione (GSH), and total glutathione (tGSH) levels. Our findings not only illuminate the nuanced response of <em>Alternaria</em> sp. 17463 to anionic stress but also underscore the fungus's potential as a bioindicator for salt stress. This research paves the way for developing targeted microbial interventions to bolster crop performance in saline environments, offering a significant step forward in precision agriculture.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"401 ","pages":"Pages 48-59"},"PeriodicalIF":4.1,"publicationDate":"2025-02-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143476681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0