Journal of biotechnology最新文献

{"title":"Substitution of the 5′-untranslated region in the alcohol oxidase 1 promoter of Komagataella phaffii alleviated the repression in the presence of glycerol","authors":"Ziwei Zhou,&nbsp;Aibo Feng,&nbsp;Wenjie Cong,&nbsp;Mingxuan Wang,&nbsp;Hualan Zhou,&nbsp;Jianguo Zhang","doi":"10.1016/j.jbiotec.2025.06.011","DOIUrl":"10.1016/j.jbiotec.2025.06.011","url":null,"abstract":"<div><div><em>Komagataella phaffii (K. phaffii)</em> alcohol oxidase 1 promoter (<em>P</em>_{<em>AOX1</em>}) is repressed in the presence of glycerol, and induced by methanol when methanol is the only carbon source, which is considered as the core feature of this cell factory for recombinant protein production. It was generally accepted that the 5´ untranslated region (5´ UTR) was involved in post-transcription processing and RNA transport. In this study, the 5′ UTR of <em>P</em>_{<em>AOX1</em>} was replaced by 5´ UTR of constitutive glyceraldehyde-3-phosphate dehydrogenase promoter (<em>P</em>_<em>GAP</em>) which was not inhibited by glycerol. Modification of <em>P</em>_{<em>AOX1</em>} alleviated its repression at the condition of mixed carbon sources of glycerol and methanol, especially at 0.67 – 1 % glycerol addition and 1 % methanol. Presence of protein encoded by <em>PAS_chr4_0626</em> was considered as the key part of <em>P</em>_{<em>AOX1</em>} repression in glycerol according to sequence analysis by JASPAR. Therefore, the substitution of the 5´ UTR or knock out of <em>PAS_chr4_0626</em> attenuated <em>P</em>_{<em>AOX1</em>} inhibition by glycerol, demonstrating the potential application of heterologous protein production under the condition of mixed carbon sources of methanol and glycerol.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"405 ","pages":"Pages 299-307"},"PeriodicalIF":4.1,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144297694","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient production of Ergothioneine via an optimized allogenous assembly of the ERG synthesis pathway in Escherichia coli BL21","authors":"Liang Li,&nbsp;Shanshan Xu,&nbsp;Yanjun Jiang","doi":"10.1016/j.jbiotec.2025.06.009","DOIUrl":"10.1016/j.jbiotec.2025.06.009","url":null,"abstract":"<div><div>Ergothioneine (ERG) is a potent antioxidant with applications in nutrition, pharmaceuticals, dermatology, and related fields. However, its large-scale production is constrained by low yields and high costs linked to extraction and chemical synthesis. Microbial fermentation offers a promising alternative. In this study, a truncated NcEgt1–1 from <em>Neurospora crassa</em> and CtEgtB from <em>Chloracidobacterium thermophilum</em> were fused to convert L-histidine and L-cysteine into hercynylcysteine sulfoxide, subsequently catalyzed by MsEgtE from <em>Mycobacterium smegmatis</em> to produce ERG. Initial expression in <em>Escherichia coli</em> yielded 87.02 mg/L of ERG. With the use of a rigid linker-EAAAK, the ERG titer increased to 144 mg/L. Further amino acid modifications in NcEgt1–1 resulted in the mutant strain M3–02, producing 325 mg/L of ERG. Fed-batch fermentation in a 5-L bioreactor achieved an ERG yield of 790 mg/L with a productivity of 9 mg/L/h. Iterative mutations of CtEgtB further increased ERG production to 478 mg/L in shake flasks and 790 mg/L in the bioreactor, with productivity rising to 14.9 mg/L/h. This study presents an engineered strain with significant ERG production potential, suitable for industrial application.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"406 ","pages":"Pages 13-23"},"PeriodicalIF":4.1,"publicationDate":"2025-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conventional to bacteria-based cancer therapy through bacterial components and drug delivery approaches: pre-clinical, clinical, and future progression.","authors":"Romi Shreshtha, Vishal Kumar Deb, Ankita Ghosh, Nidhi Chauhan, Utkarsh Jain","doi":"10.1016/j.jbiotec.2025.06.010","DOIUrl":"https://doi.org/10.1016/j.jbiotec.2025.06.010","url":null,"abstract":"<p><p>Cancer is one of the current unwinnable conflicts, despite the abundance of treatments available. Traditional treatments like chemotherapy, radiation therapy, and surgery have been used for many years, but they are insufficient against advanced tumors. Small molecules, supramolecular complexes, modified immune cells, and plant-based medicines have all contributed to significant improvements in cancer therapy. However, certain undesirable activities and obstacles are on the horizon. To overcome such problems, research into bacterial strain-based cancer therapy has recently begun in both preclinical and clinical settings. In this context, many bacterial strains are used after genetic modification or as an innovative and smart delivery system. In this manuscript, we reviewed and discussed several bacterial components with anti-tumor and anti-cancerous properties, immunotherapeutic properties, and their products, such as endotoxins, peptides, etc., as well as genetically modified bacterial strains as therapeutic drug-delivery agents in cancer treatments or in conjunction with other cancer therapies. Herein, state-of-the-art advances, shortcomings of these therapies, and future advancements in bacteria-based therapies are also discussed.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144302168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Branched-chain polyamine beads for sensitive recovery and detection of low-copy DNA in aqueous solutions","authors":"Shinsuke Fujiwara ,&nbsp;Emi Kawamori ,&nbsp;Himari Aoki ,&nbsp;Yukiko Nakura ,&nbsp;Yuri Ishii ,&nbsp;Sadahiro Masuo ,&nbsp;Kiyoshi Yasukawa ,&nbsp;Itaru Yanagihara","doi":"10.1016/j.jbiotec.2025.06.008","DOIUrl":"10.1016/j.jbiotec.2025.06.008","url":null,"abstract":"<div><div><em>N</em>⁴-bis(aminopropyl)spermidine (BCPA), a branched-chain polyamine, is uniquely found in hyperthermophiles that thrive above 80°C. Compared to linear polyamines such as spermidine and spermine, BCPA induces DNA compaction at significantly lower concentrations and precipitates DNA more efficiently. To harness these properties, BCPA was immobilized onto <em>N</em>-hydroxysuccinimide (NHS)-activated magnetic microbeads, and its DNA recovery efficiency was evaluated using Salmon sperm DNA. BCPA-conjugated beads exhibited superior DNA-binding capacity compared to spermidine-conjugated and conventional silica beads, with bound DNA remaining unreleased upon treatment with 0.5 % sodium dodecyl sulfate (SDS), 2 mM ATP, or 2 mM phosphate (Pi) at pH 8.8. However, efficient DNA release was achieved with 2 mM pyrophosphate (PPi) at pH 8.0 or 1 mM PPi at pH 10.3. This property enables direct DNA amplification without a separate release step, as dNTPs used in PCR generate PPi as a byproduct, facilitating DNA detachment. To assess the beads' applicability for low-copy DNA detection, plasmid DNA containing the <em>Ureaplasma parvum</em> 16S rRNA region was prepared in saline at varying concentrations. BCPA-conjugated beads successfully recovered and directly amplified as few as 10³ copies of plasmid DNA from a 10-mL saline solution, whereas the same amount remained undetectable using conventional magnetic beads ethanol precipitation. These findings demonstrate the potential of BCPA-conjugated beads for efficient DNA capture and direct amplification, with promising applications in clinical diagnostics and environmental DNA monitoring.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"405 ","pages":"Pages 290-298"},"PeriodicalIF":4.1,"publicationDate":"2025-06-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cellular engineering strategies for soluble and secretory recombinant human insulin production in Pseudomonas fluorescens and batch bioreactor study","authors":"Ansuman Sahoo ,&nbsp;Venkata Dasu Veeranki ,&nbsp;Sanjukta Patra","doi":"10.1016/j.jbiotec.2025.06.007","DOIUrl":"10.1016/j.jbiotec.2025.06.007","url":null,"abstract":"<div><div>One of the main challenges in expressing recombinant human insulin is the formation of inclusion bodies. In our previous study, we successfully expressed insulin in <em>Pseudomonas fluorescens</em>. We observed that lowering the post-induction temperature enhanced the soluble fraction; however, it resulted in lower protein titer. In this study, the individual and synergistic effects of multiple chaperones and signal peptides on soluble protein synthesis are analyzed. A plasmid with a spectinomycin antibiotic resistance encoding gene was constructed for co-expression of chaperones. The combined effect of Disulphide bond protein A (DsbA) and phosphate binding protein (Pbp) resulted in ∼60 % of the fusion protein in soluble form. A comprehensive study was performed to assess the influence of the location of the His-tag on the expression and solubility of the protein. A high-copy origin of replication (ori) produced a lower soluble protein titer and caused a significantly longer lag phase. Under optimal concentration of antibiotics and inducer, inoculation percentage and induction OD₆₀₀, the protein and soluble protein fraction titer increased by ∼58 % and ∼27 %, respectively. In a batch bioreactor, a maximum of 234.58 mg/l fusion protein with 71.59 % solubility and around 15 % of the total soluble protein fraction in the culture supernatant was obtained. Circular dichroism analysis revealed that the secondary structure of the purified insulin was comparable to that of the standard insulin. To the best of our knowledge, this is the first study reporting the combinatorial effect of chaperones and signal peptides on the production of human insulin in the soluble form.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"406 ","pages":"Pages 1-12"},"PeriodicalIF":4.1,"publicationDate":"2025-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144293822","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leaf vein scaffolds for three-dimensional culture of PDLSCs-derived Muse cells","authors":"Xueting Bi ,&nbsp;Qian Lan ,&nbsp;Xin Xiao ,&nbsp;Yu Deng ,&nbsp;Dongsheng Li","doi":"10.1016/j.jbiotec.2025.06.005","DOIUrl":"10.1016/j.jbiotec.2025.06.005","url":null,"abstract":"<div><div>Periodontal disease, a significant global health burden, has encountered limited success with current therapeutic strategies to achieve full tissue regeneration. The emergence of Multilineage Differentiation and Stress-Enduring (Muse) cells presents a promising avenue for periodontal tissue regeneration. This study introduced a novel three-dimensional (3D) culture system utilizing Magnolia leaf vein scaffolds, characterized for their biocompatibility and evaluated for their impact on Muse cells' proliferation, adhesion, osteogenic differentiation, and exosome secretion. The isolation of Muse cells from Periodontal Ligament Stem Cells (PDLSCs) was successfully accomplished, with excellent compatibility observed with the plant-derived scaffolds. Notably, the 3D culture substantially upregulated osteogenic markers and promoted the formation of mineralized nodules, signifying enhanced osteogenic potential. Additionally, Muse cells in 3D culture exhibited a significant increase in exosome secretion, which were more effective in stimulating PDLSCs proliferation. The study concluded that plant leaf vein scaffolds provide a sustainable and effective platform for 3D stem cell culture, with the potential to significantly enhance the therapeutic efficacy of Muse cells in periodontal tissue engineering.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"405 ","pages":"Pages 275-282"},"PeriodicalIF":4.1,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel immobilized bioreactor based on CFD simulation for fuel ethanol production from corn","authors":"Qingguo Liu ,&nbsp;Jing Liu ,&nbsp;Alan Yan ,&nbsp;Wenjun Sun ,&nbsp;Caice Liang ,&nbsp;Tianpeng Chen ,&nbsp;Qingshi Wen ,&nbsp;Yanjun Chen ,&nbsp;Hanjie Ying ,&nbsp;Yong Chen","doi":"10.1016/j.jbiotec.2025.06.003","DOIUrl":"10.1016/j.jbiotec.2025.06.003","url":null,"abstract":"<div><div>This study evaluated the feasibility of ethanol production from corn in a surface immobilized bioreactor. The mass transfer of 50 L immobilized bioreactor was analyzed based on computational fluid dynamics (CFD) simulation. Compared to the traditional stirred fermenter, the surface immobilized fermenter system exhibited a relatively weak liquid velocity, but its flow field distribution was relatively uniform, which ensured an ideal interaction environment between cells and substrates. The difference of fermentation indexes between the two fermentation methods was then verified by ethanol fermentation using corn hydrolysate. It was found that the ethanol productivity (P) of immobilized cells was increased by 20.59 %, while the amount of corn consumed for one ton of ethanol was decreased by 5.90 %. To further enhance industrial application, the semi-continuous fermentation was adopted, the P and ethanol yield were increased by 30.88 % and 3.06 percentage points, respectively, compared to free-cell fermentation (FCF). This work demonstrated that the mass transfer issues associated with traditional immobilized-cell fermentation (ICF), such as embedding and chemical crosslinking, were resolved through bioreactor design and process optimization. Meanwhile, the surface immobilization technology showcased special advantages, including a broad spectrum of raw materials and strong process stability, indicating great industrial application potential in ethanol production.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"405 ","pages":"Pages 254-262"},"PeriodicalIF":4.1,"publicationDate":"2025-06-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144239563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Brazilian Amazon red propolis as a sustainable resource for the green synthesis of antibacterial silver nanoparticles","authors":"Roberto Pereira Santos ,&nbsp;Jaqueline Daniele Santos Barros ,&nbsp;Euzinete Borges Pereira ,&nbsp;Karla Gabriela Mota de Oliveira ,&nbsp;Gabriel Sousa Brito ,&nbsp;Fernanda Farias Costa ,&nbsp;Queli Cristina Fidelis ,&nbsp;Aramys Silva Reis ,&nbsp;Carlos Alexandre Holanda ,&nbsp;Richard Pereira Dutra","doi":"10.1016/j.jbiotec.2025.05.018","DOIUrl":"10.1016/j.jbiotec.2025.05.018","url":null,"abstract":"<div><div>This study investigates the use of Amazon red propolis for the green synthesis of antibacterial silver nanoparticles (AgNPs). A hydroalcoholic propolis extract was fractionated to yield fractions rich in phenolic compounds. High-performance liquid chromatography identified calycosin as a chemical marker, along with liquiritigenin, isoliquiritigenin, and formononetin. The propolis extract and the chloroform fraction inhibited <em>Staphylococcus aureus</em> growth with a bactericidal effect, while for <em>Escherichia coli</em>, a bacteriostatic effect was observed at concentrations below 1000 µg/mL. The AgNPs were characterized by UV-Vis spectroscopy, confirming surface plasmon resonance, and their morphology and size were analyzed using dynamic light scattering and transmission electron microscopy, with elemental composition assessed via energy-dispersive spectroscopy. X-ray diffraction and selected area electron diffraction showed the crystalline structure. Fourier-transform infrared spectra indicated that carboxyl and hydroxyl groups in flavonoids reduce silver ions and contribute to the nanoparticles' chemical stability. The nanoparticles synthesized from the propolis extract showed enhanced antibacterial potential, with efficacy against <em>E. coli</em> (MIC 25 µg/mL, MBC 50 µg/mL) compared to <em>S. aureus</em> (MIC 100 µg/mL, MBC 100 µg/mL), and demonstrated selectivity for Gram-negative bacteria over murine macrophages (CC₅₀ 83.92 μg/mL). These nanoparticles exhibited maximum absorption in the UV-Vis region at 418 nm, with a spherical silver core encapsulated by compounds from propolis, hydrodynamic diameter of 252.47 ± 1.42 nm, a zeta potential of −51.80 ± 0.70 mV, and a polydispersity index of 0.218 ± 0.01, indicating high chemical stability. These findings underscore Amazon red propolis as a sustainable resource for developing selective and stable antibacterial agents.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"405 ","pages":"Pages 263-274"},"PeriodicalIF":4.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultivation of Nannochloropsis oceanica using food grade industrial side streams: Effect on growth and relative abundance of selected amino acids and unsaturated fatty acids","authors":"Emil Gundersen,&nbsp;Charlotte Jacobsen","doi":"10.1016/j.jbiotec.2025.06.006","DOIUrl":"10.1016/j.jbiotec.2025.06.006","url":null,"abstract":"<div><div>Developing more resource-efficient food production is needed to sustainably support a growing world population. Microalgae present a promising, future source of food ingredients, especially if cultivated using industrial side streams. In this study, <em>Nannochloropsis oceanica</em> was cultivated using food grade process water from industrial enzyme production as a source of inorganic nutrients. Effects on growth and the nutritional quality of produced biomass, with focus on selected fatty acids and amino acids, were investigated. One of the two tested side streams replaced up to 40% of a commercial nutrients supplement with no negative effect on either growth or the content of nutritionally important amino- and fatty acids. The other tested side stream hampered both growth and significantly reduced the content of omega-3 fatty acids in the biomass at only 20% replacement level. The diverging results are likely caused by differences in the macro- and micronutrient profile, but further investigation into the side streams composition is needed to unravel the exact cause. If optimized, the use of such side streams can help reduce both the resource input and cost of producing <em>N. oceanica</em> as a future, sustainable source of food ingredients.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"405 ","pages":"Pages 249-253"},"PeriodicalIF":4.1,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144239407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deletion of bikaverin and fusarubin biosynthesis gene clusters via CRISPR/Cas9 system in Fusarium fujikuroi and its effect on GA3 biosynthesis","authors":"Lianggang Huang ,&nbsp;Yixin Song ,&nbsp;Ningning Li ,&nbsp;Jie Gao ,&nbsp;Bo Zhang ,&nbsp;Zhiqiang Liu ,&nbsp;Yuguo Zheng","doi":"10.1016/j.jbiotec.2025.06.004","DOIUrl":"10.1016/j.jbiotec.2025.06.004","url":null,"abstract":"<div><div>Gibberellic acid (GA3) is a critical plant hormone with significant agricultural applications, yet its production in <em>Fusarium fujikuroi</em> is constrained by competition for metabolic precursors, particularly acetyl-CoA, which is essential for GA3 biosynthesis. The genome of <em>F. fujikuroi</em> harbors numerous secondary metabolite biosynthetic gene clusters that divert acetyl-CoA away from the GA3 pathway, thereby limiting its yield. To address this challenge, we employed the CRISPR/Cas9 system to delete the bikaverin and fusarubin biosynthesis gene clusters, which are known to compete with GA3 biosynthesis for acetyl-CoA. This genetic intervention resulted in a substantial increase in GA3 production, with the ΔBIKΔFSR strain yielding 31.67 % more GA3 compared to the wild-type strain. Notably, the deletion of these gene clusters not only enhanced GA3 biosynthesis but also improved mycelial growth and carbon assimilation, as evidenced by increased consumption of reducing sugars during fermentation. We further employed qRT-PCR to assess comparative expression levels of genes associated with the glycohydrolysis, glycolysis, and the TCA pathway in engineered strain. Results indicated that removing by-product gene clusters enhances the glycohydrolase system, accelerating carbon assimilation. Given the presence of dozens of secondary metabolite biosynthetic gene clusters in the <em>F. fujikuroi</em> genome, the strategy reported here offers a promising avenue for further enhancing GA3 production by targeting additional non-essential gene clusters.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"405 ","pages":"Pages 229-237"},"PeriodicalIF":4.1,"publicationDate":"2025-06-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144222429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}