Journal of biotechnology最新文献

{"title":"Acid-triggered ultralong hydrogen release from AB-loaded hollow mesoporous silica nanoparticles enhances salt tolerance in sweetpotato","authors":"Nan Xia ,&nbsp;Zheng Zhuang ,&nbsp;Qi Wang ,&nbsp;Zhiyuan Pan ,&nbsp;Yicheng Yu ,&nbsp;Zongyun Li ,&nbsp;Jian Sun ,&nbsp;Yanjuan Li","doi":"10.1016/j.jbiotec.2025.07.008","DOIUrl":"10.1016/j.jbiotec.2025.07.008","url":null,"abstract":"<div><div>Soil salinization severely restricts crop growth and productivity worldwide. Hydrogen (H₂) has demonstrated considerable potential in enhancing plant stress tolerance. However, its agricultural application is limited by the rapid dissipation of H₂ in traditional electrolysis-generated hydrogen-rich water (HRW). In this work, we synthesized a nanoscale H₂-releasing material (AB@HMSN) by encapsulating ammonia borane (AB) into hollow mesoporous silica nanoparticles (HMSN), achieving ultrahigh AB loading capacity (811 mg·g⁻¹ HMSN) and acid-responsive sustained H₂ release behavior in solution. The H₂ release duration reached 75 h, significantly exceeding that of conventional HRW. When applied to salt-stressed (150 mM NaCl) sweetpotato (<em>Ipomoea batatas</em> (L.) Lam) seedlings, 16 mg·L⁻¹ AB@HMSN significantly improved chlorophyll content, with chlorophyll a, chlorophyll b, and total chlorophyll increasing by 80 %, 100 %, and 88 %, respectively. Photosynthetic performance was markedly enhanced, showing increases of 53 %, 26 %, and 24 % in photosynthetic rate (Pn), stomatal conductance (Gs), and transpiration rate (Tr), respectively. Additionally, AB@HMSN treatment strengthened antioxidant defenses by elevating the activities of key enzymes, including superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), while reducing the accumulation of reactive oxygen species (H₂O₂ and ·O^2-). Mechanistically, AB@HMSN induced endogenous melatonin (MT) production, which activated sodium-proton (Na⁺/H⁺) antiporters and plasma membrane proton-pumping ATPase (PM H⁺-ATPase) activity. This process restored ion homeostasis by facilitating Na⁺ efflux, K⁺ influx, and H⁺ influx, thereby enhancing salt tolerance. These findings not only advance our understanding of hydrogen-mediated stress tolerance but also offer a practical nanomaterial-based strategy for sustainable agriculture in saline environments.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"406 ","pages":"Pages 105-114"},"PeriodicalIF":4.1,"publicationDate":"2025-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional nucleic acid-powered hybrid nanocarriers for synchronized targeted delivery of dual-solubility therapeutics","authors":"Xin Liu ,&nbsp;Mengqiu Sun ,&nbsp;Lu Dai ,&nbsp;Yusheng Li ,&nbsp;Rui Song ,&nbsp;Hong Bian ,&nbsp;Hao Zhang","doi":"10.1016/j.jbiotec.2025.07.002","DOIUrl":"10.1016/j.jbiotec.2025.07.002","url":null,"abstract":"<div><div>Combination chemotherapy presents a promising approach to overcome multidrug resistance in cancer treatment. However, conventional delivery systems struggle to achieve synchronized co-delivery of hydrophilic and hydrophobic therapeutics with tumor-specific targeting and controlled release. Here, we developed a multifunctional nanocarrier integrating poly (lactic-co-glycolic acid) nanoparticles with polydopamine and programmable nucleic acids. Within this system, the hydrophobic core encapsulates lipophilic drugs, while the surface nucleic acid architecture enables hydrophilic drug loading via molecular intercalation. Furthermore, tumor-targeting aptamers guide selective cellular uptake, and a tumor microenvironment-responsive mechanism ensures spatiotemporally controlled drug release. As demonstrated experimentally, this unified platform demonstrates promising antitumor activity with potential advantages in drug delivery efficiency. By combining targeted delivery, differential drug loading, and stimuli-triggered release, the design establishes a modular framework for precision combination therapies, thereby advancing adaptable nanomedicine in oncology.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"406 ","pages":"Pages 127-135"},"PeriodicalIF":4.1,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel approaches to engineering autolysis: A scalable alternative for recovery of bioproducts.","authors":"Kyle Gaius Jonsson,&nbsp;Darren Nesbeth,&nbsp;Eli Keshavarz-Moore","doi":"10.1016/j.jbiotec.2025.07.007","DOIUrl":"10.1016/j.jbiotec.2025.07.007","url":null,"abstract":"<div><div>For intracellularly expressed bioproducts, cell disruption is an essential unit operation in the bioprocess. Traditional cell lysis techniques, such as homogenisation or chemical lysis, may result in product damage due to the shear forces or the harsh chemicals used, respectively. Autolysis, or the expression of lytic proteins to trigger self-lysis, presents as an alternative approach for intracellular bioproduct release. However, challenges of using autolysis in bioprocessing have hindered the wide adoption of the technique. There are three main issues related to autolysis: leaky gene expression leading to reduced performance in fermentation, low lysis efficiencies compared to traditional methods, and a lack of consideration for scale-up in the current literature. This review summarises these challenges and discusses the potential solutions.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"406 ","pages":"Pages 115-126"},"PeriodicalIF":4.1,"publicationDate":"2025-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144604305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microwave-assisted biogenic synthesis of pure copper oxide nanoparticles by Lepidium sativum L. leaves extract: Characterization and evaluation of antibacterial activity","authors":"Ahmed A. Ibrahim","doi":"10.1016/j.jbiotec.2025.07.003","DOIUrl":"10.1016/j.jbiotec.2025.07.003","url":null,"abstract":"<div><div>This study delineates a sustainable and environmentally compatible route for the biogenic synthesis of copper oxide nanoparticles (CuO-NPs) utilizing the leaf extract of <em>Lepidium sativum L</em>. as a biocompatible reducing agent, employing microwave technology, alongside an evaluation of their antibacterial activity and biofilm formation against <em>Klebsiella pneumoniae, Pseudomonas aeruginosa, Escherichia coli,</em> and <em>Streptococcus mutans</em>. The size, shape, crystalline structure, and stability of the synthesized CuO-NPs were determined by energy dispersive X-ray (EDX), scanning electron microscopy (SEM), X-ray diffraction (XRD), infrared (IR) and ultraviolet-visible (UV-Visible), dynamic light scattering (DLS), and X-ray photoelectron spectroscopy (XPS). The stability of the synthesized CuO-NPs exhibited exceptional stability within 90 days. XRD confirmed that the synthesized CuO-NPs had a spherical morphology, with an average particle diameter of 30 nm. Furthermore, CuO-NPs displayed strong antibacterial activity against the bacterial strains, as well as significant prevention of biofilm formation. This study's findings confirmed the successful synthesis of an effective, economically viable, and eco-friendly synthesis method for CuO-NPs. The results reveal their substantial antibacterial activities, indicating their use as potential alternatives to standard antimicrobial therapies, with major advantages in terms of cost and biocompatibility.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"406 ","pages":"Pages 82-90"},"PeriodicalIF":4.1,"publicationDate":"2025-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144583999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of 3D-Printed Acrylonitrile Butadiene Styrene and Polylactic Acid Carrier-beads for Gluconobacter oxydans Immobilization.","authors":"Ricardo Gonzalo Ramírez Brenes, Isabella Maria Tenório Soares Santos, Livia da Silva Chaves de Carvalho, Ninoska Isabel Bojorge Ramírez, Nei Pereira","doi":"10.1016/j.jbiotec.2025.07.001","DOIUrl":"https://doi.org/10.1016/j.jbiotec.2025.07.001","url":null,"abstract":"<p><p>Bioprocesses play a crucial role in the production of biotechnological compounds, and 3D printing offers innovative solutions, including the creation of beads for cell immobilization. This technology enables the development of customized structures with various geometries and properties (shape, size, porosity, density, and roughness). This study produced carrier beads using fused filament fabrication with two different thermopolymers: acrylonitrile butadiene styrene (ABS) and polylactic acid (PLA). The carrier beads were printed with three distinct millipore geometries-hexagonal, square, and triangular-to evaluate immobilizing Gluconobacter oxydans' efficiency and investigate how immobilization varies based on the material type and millipore geometry. ABS exhibited greater surface roughness compared to PLA, which enhanced cell immobilization. The highest cell immobilization efficiencies were achieved with the ABS beads, with the hexagonal millipore geometry demonstrating the best efficiency at 84.7%. Determining mechanical parameters, including storage and loss modulus, is essential for ensuring the durability of the beads in industrial processes. The glass transition temperatures for ABS and PLA were found to be 105 °C and 60 °C to 65 °C, respectively. Thus, 3D printing stands out for its versatility in material selection and geometry, optimizing the performance of bioprocesses.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":""},"PeriodicalIF":4.1,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient genome engineering in Agrobacterium tumefaciens C58 using recombineering assisted by CRISPR/Cas9","authors":"Shiqing Gao ,&nbsp;Xiuling Wang ,&nbsp;Runyu Yang,&nbsp;Zhilong Bian,&nbsp;Daojing Yong,&nbsp;Shouying Xu,&nbsp;Liujie Huo,&nbsp;Qiang Tu,&nbsp;Youming Zhang,&nbsp;Jun Fu,&nbsp;Ruijuan Li","doi":"10.1016/j.jbiotec.2025.07.005","DOIUrl":"10.1016/j.jbiotec.2025.07.005","url":null,"abstract":"<div><div>Recombineering, a technique derived from phage-encoded homologous recombination, has emerged as a vital approach for bacterial genome engineering. <em>Agrobacterium tumefaciens</em> is extensively utilized to transfer DNA into the host plant genomes. To facilitate the transformation of various plant species, particularly those of considerable economic value, genetic modifications of <em>Agrobacterium</em> strains are essential. Our previous studies established an <em>Agrobacterium</em>-specific phage-encoded homologous recombination system for <em>Agrobacterium</em> species. Yet, recent investigations have indicated that there is a substantial variability in the recombination efficiency of these recombineering systems for gene editing across different genome loci in <em>A. tumefaciens</em>. In this work, we present the development of an efficient genome engineering tool for <em>A. tumefaciens</em> by integrating recombineering with CRISPR/Cas9 technology. Initially, we found that lengthening the homology arms significantly enhanced genome editing efficiency. Nevertheless, at certain genomic sites, even when the length of the homology arms was increased, the editing efficiency remained suboptimal. Subsequently, combination of the <em>Agrobacterium</em>-specific recombineering system with the CRISPR/Cas9 system markedly enhanced the genome engineering efficiency. This study offers an enhanced and efficient genome engineering tool for <em>A. tumefaciens</em>, which could potentially be applied to other species within the <em>Agrobacterium</em> genus.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"406 ","pages":"Pages 99-104"},"PeriodicalIF":4.1,"publicationDate":"2025-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High-throughput flow cytometry-based titration of a live attenuated rotavirus vaccine","authors":"Mafalda M. Dias ,&nbsp;Sofia B. Carvalho ,&nbsp;Jean-Philippe Matheise ,&nbsp;Guillaume Van Beersel ,&nbsp;Isabelle Knott ,&nbsp;Patrícia Gomes-Alves ,&nbsp;Paula M. Alves","doi":"10.1016/j.jbiotec.2025.07.004","DOIUrl":"10.1016/j.jbiotec.2025.07.004","url":null,"abstract":"<div><div>Vaccine potency is a critical quality attribute to ensure vaccine’s safety and efficacy. The standard potency assay for vaccines is the traditional cell culture infectious dose 50 % (CCID₅₀). However, this method often yields highly variable results, presenting low sensitivity and requiring a lengthy analysis. Here, we report a flow cytometry-based method for potency assessment of the Rotarix rotavirus vaccine. This method quantifies infected cells by detecting VP7 viral protein in MA104 cells, integrating fluorescence-automated cell counting with a high-throughput system. Determination of adequate cell seeding timepoint, target cell concentration and endpoint after infection are key parameters for the precision, robustness, and accuracy of titer determination. We established optimized assay conditions for lyophilized and final vaccine formulations. Data from both workflows are in line with the CCID₅₀ range of concentrations. The coefficient of variation for inter- and intra-assay for both formulations is ≈ 2 %. Moreover, the flow cytometry method reduced the hands-on and analysis time by at least 4 days, offering a rapid high-throughput alternative tool to streamline the challenges associated with potency testing for viral-based vaccines, impacting therapeutics time to market.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"406 ","pages":"Pages 91-98"},"PeriodicalIF":4.1,"publicationDate":"2025-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144567470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved methanol utilization of Komagataella phaffii by rebuilding metabolic network based on new alcohol-aldehyde transformation system","authors":"Kang Li ,&nbsp;Xiuxia Liu ,&nbsp;Chunli Liu ,&nbsp;Zhonghu Bai ,&nbsp;Shaojie Yang ,&nbsp;Yankun Yang","doi":"10.1016/j.jbiotec.2025.06.013","DOIUrl":"10.1016/j.jbiotec.2025.06.013","url":null,"abstract":"<div><div>Bioconversion based on methanol holds promise for advancing global carbon neutrality and mitigating the global food crisis. However, the limited efficiency of methanol utilization hampers methanol-based biomanufacturing in microorganisms. This study focuses on enhancing methanol utilization efficiency in <em>Komagataella phaffii</em> through the implementation of a novel alcohol-aldehyde conversion system based on the Adh900 protein. Results indicate a notable 14 % increase in biomass compared to the wild-type strain. Furthermore, analysis of the amino acid metabolism pathway underscores the significance of histidine in facilitating the methanol utilization pathway (MUTP). By combination of adding 120 mg/L histidine to the medium and optimizing promoter strategies, the dry cell weight (DCW) was up to 4.33 g DCW/L, which was increased by 63 %. The combination of these strategies has important research significance for methanol-based biological metabolism, and will further promote the microbial application of methanol. These findings are poised to advance the development of methanol-nutritive cell factories, thereby propelling the evolution of methanol biomanufacturing technology.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"406 ","pages":"Pages 60-70"},"PeriodicalIF":4.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557586","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of chikungunya virus neutralising monoclonal antibodies expressed in tobacco plants","authors":"Catherine M. Moore ,&nbsp;Mathew J. Paul ,&nbsp;Elizabeth Pinneh ,&nbsp;Balamurugan Shanmugaraj ,&nbsp;James Ashall ,&nbsp;Sathishkumar Ramalingam ,&nbsp;Roger Hewson ,&nbsp;Michael S. Diamond ,&nbsp;Julie M. Fox ,&nbsp;Julian K.-C. Ma","doi":"10.1016/j.jbiotec.2025.06.014","DOIUrl":"10.1016/j.jbiotec.2025.06.014","url":null,"abstract":"<div><div>Chikungunya virus (CHIKV) causes a debilitating musculoskeletal disease, characterised by flu-like symptoms, rash, and severe joint pain, which can last for months, even after the resolution of infection. Although the first CHIKV vaccine was approved in the USA in 2023 for use in adults, there is currently no specific antiviral therapy for infection. While neutralising antibody-based prophylactic and therapeutic agents have been considered, affordability and accessibility are major barriers to global regions where Chikungunya disease is epidemic. Here, we expressed five anti-CHIKV neutralising IgG monoclonal antibodies (mAbs) in <em>N. benthamiana</em> plants to investigate the potential use of this manufacturing platform. Plants produced IgG mAbs that compared favourably to mammalian cell-expressed antibodies, including for binding kinetics to CHIKV antigens and neutralisation activity. The yields of mAbs from plants were variable, as three of the antibodies’ yields would need further expression optimisation to warrant future development. The successful expression of these antibodies in <em>N. benthamiana</em> plants supports the growing pipeline of Global Health product targets that could be developed using a highly transferable, low-cost, low-tech plant production platform in resource-poor countries.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"406 ","pages":"Pages 53-59"},"PeriodicalIF":4.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144557574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced cascade biosynthesis of D-phenyllactic acid using metal-organic framework-encapsulated co-expressing E. coli","authors":"Xi Luo ,&nbsp;Yufeng Zhou ,&nbsp;Yaping Yang ,&nbsp;Gaowei Hu ,&nbsp;Fengwei Yin ,&nbsp;Longfei Yin ,&nbsp;Yingying Zhang ,&nbsp;Yongqian Fu","doi":"10.1016/j.jbiotec.2025.06.015","DOIUrl":"10.1016/j.jbiotec.2025.06.015","url":null,"abstract":"<div><div>Phenylpyruvic acid, as a versatile organic acid, has attracted widespread attention in the fields of food, feed, pharmaceuticals, and cosmetics for its synthetic methods. In this study, we co-expressed L-amino acid oxidase, D-lactate dehydrogenase, and glucose dehydrogenase in <em>Escherichia coli</em>. The resulting recombinant whole-cell biocatalysts exhibited high efficiency in the cascade enzymatic synthesis of D-phenyllactic acid from L-phenylalanine. However, their limitations in operational stability and reusability have impeded their broader application. To address this issue, the co-expressing bacteria was immobilized on the metal-organic framework (MOF) ZIF-90. The encapsulation rate of the immobilized <em>E</em>. <em>coli</em> cells (<em>E</em>. <em>coli</em>@ZIF-90) and the recovery rate of their catalytic activity were 94.8 % and 92.7 %, respectively. The physical and biochemical properties of the <em>E</em>. <em>coli</em>@ZIF-90 were subsequently studied in detail. Compared with free cells, <em>E</em>. <em>coli</em>@ZIF-90 demonstrates superior stability under acidic and alkaline conditions, enhanced thermal stability, and increased tolerance to metal ions and organic reagents. After storage at 4°C for 8 days, the residual enzyme activity of the immobilized cells is still over 75 %, which is about 1.7 times that of free cells. Following 10 cycles of biocatalytic reactions, the immobilized cells maintained over 80 % of their enzyme activity, contrasting with the 58.6 % residual activity observed in free cells. In the enzyme cascade reaction catalyzed by <em>E</em>. <em>coli</em>@ZIF-90, 25 g·l⁻¹ L-PHE was completely reacted to produce D-PLA 17.8 g·l⁻¹, with an enantiomeric excess (<em>e</em>.<em>e</em>.) greater than 99.5 %. This study provides a promising method for obtaining efficient and robust biocatalysts for the biosynthesis of D-PLA. Additionally, it provides essential insights that are extendable to the synthesis of a broader range of compounds.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"406 ","pages":"Pages 71-81"},"PeriodicalIF":4.1,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144564832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}