Journal of biotechnology最新文献

Control of alcoholic fermentation through modulation of nitrogen metabolism in Saccharomyces cerevisiae 通过调节酿酒酵母的氮代谢控制酒精发酵

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-05-20 DOI: 10.1016/j.jbiotec.2025.05.015

Naoki Akasaka , Yukiko Sugimoto , Takuma Kajihara , Hiroshi Takagi , Daisuke Watanabe

{"title":"Control of alcoholic fermentation through modulation of nitrogen metabolism in Saccharomyces cerevisiae","authors":"Naoki Akasaka , Yukiko Sugimoto , Takuma Kajihara , Hiroshi Takagi , Daisuke Watanabe","doi":"10.1016/j.jbiotec.2025.05.015","DOIUrl":"10.1016/j.jbiotec.2025.05.015","url":null,"abstract":"<div><div><em>Saccharomyces cerevisiae</em> sake strains exhibit high alcoholic fermentation performance. Comparative transcriptomic analysis revealed that the expression of genes required for nitrogen sensing and metabolism, including amino acid biosynthesis and uptake, was markedly lower in the sake strain than in the laboratory strain. Thus, we hypothesized that changes in nitrogen metabolism affect the fermentation capability of <em>S. cerevisiae</em>. To evaluate the impact of altered nitrogen metabolism on alcoholic fermentation, we focused on the transcription activators Gcn4p, Gln3p, and Gat1p, and the protein kinase Npr1p, all of which are key regulators controlling expression of genes for amino acid biosynthesis and uptake responding to nitrogen availability. Fermentation tests demonstrated that laboratory strain-derived single-deletion mutants of the regulator genes exhibited higher fermentation performance than the parental strain, which was accompanied by decrease in intracellular amino acid levels in the mutants. Disruption of the genes encoding glutamate dehydrogenases, which play a central role in nitrogen assimilation, also enhanced the fermentation rate. A Greatwall family protein kinase Rim15p inhibits alcoholic fermentation by diverting carbon flux from glycolysis to the synthesis of 1,3-β-glucan, a major cell wall component. Since the content of 1,3-β-glucan was unaffected by disruption of the regulator genes, the elevated fermentation performance of the disruptants was accomplished independently of the signaling pathway governed by Rim15p. The high fermentation rate of the disruptants might be attributed to increased carbon entry into glycolysis caused by the compromised biosynthesis of amino acids, which are synthesized from intermediary metabolites of glycolysis and tricarboxylic acid cycle.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"405 ","pages":"Pages 159-168"},"PeriodicalIF":4.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144116338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating the influence of process parameters on the properties and refolding yield of single-chain variable fragment inclusion bodies 研究了工艺参数对单链可变片段包涵体性质和再折叠产率的影响。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-05-20 DOI: 10.1016/j.jbiotec.2025.05.014

Mohamed Elshazly , Benedikt Leeb , Eva Prada Brichtova , Florian Gisperg , Robert Klausser , Shilpa Vijayakumar , Bernhard Lendl , Martin Voigtmann , Matthias Berkemeyer , Oliver Spadiut , Julian Kopp

{"title":"Investigating the influence of process parameters on the properties and refolding yield of single-chain variable fragment inclusion bodies","authors":"Mohamed Elshazly , Benedikt Leeb , Eva Prada Brichtova , Florian Gisperg , Robert Klausser , Shilpa Vijayakumar , Bernhard Lendl , Martin Voigtmann , Matthias Berkemeyer , Oliver Spadiut , Julian Kopp","doi":"10.1016/j.jbiotec.2025.05.014","DOIUrl":"10.1016/j.jbiotec.2025.05.014","url":null,"abstract":"<div><div>Ever since the potential of inclusion bodies (IBs) has been recognized, substantial advances have been made towards understanding IB processes and enabling efficient and controlled development strategies. Still, the influence of the chosen upstream processing (USP) strategy on the properties of inclusion bodies (IBs) and their refolding performance remains poorly understood. This work aims to target this challenge by investigating the influence of two chosen USP parameters, namely the specific substrate uptake rate and the temperature during induction, on IB titer, IB properties, namely IB purity, size and secondary protein structure of the IBs, as well as refolding yield of single-chain variable fragment M (scFvM) IBs. Contrary to findings in the literature, USP conditions neither had a statistically significant effect on the aforementioned IB properties nor on the refolding yield, but could clearly alter the IB titer. Our results provide detailed analytical insights on the independence of IB properties from USP conditions for this protein, while increasing the volumetric IB productivity proved feasible through variations in USP parameters. Therefore, titer maximization appears to be the sole optimization strategy for scFvM IBs and these findings may also apply to other target proteins with similar structural properties.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"405 ","pages":"Pages 182-190"},"PeriodicalIF":4.1,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144127738","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and engineering of a sucrose synthase from Stevia rebaudiana for glycosylation applications 甜菊糖基化蔗糖合成酶的鉴定与工程研究。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-05-14 DOI: 10.1016/j.jbiotec.2025.05.011

Kai Chen , Yuan Liao , Xiaona Chen , Kecai Chen , Weicai Song , Liping Zhu , Yan Li , Honghua Jia

{"title":"Identification and engineering of a sucrose synthase from Stevia rebaudiana for glycosylation applications","authors":"Kai Chen , Yuan Liao , Xiaona Chen , Kecai Chen , Weicai Song , Liping Zhu , Yan Li , Honghua Jia","doi":"10.1016/j.jbiotec.2025.05.011","DOIUrl":"10.1016/j.jbiotec.2025.05.011","url":null,"abstract":"<div><div>Sucrose synthase (SuSy) is a unique glycosyltransferase that can be utilized in the production of nucleoside monosaccharides, such as diphosphate (UDP)-glucose, which serve as essential sugar donors for the glycosylation reactions catalyzed by UDP-dependent glycosyltransferases (UGTs). The selection of an appropriate SuSy coupled with a UGT is crucial for achieving the efficient synthesis of glycoside products. In this study, three candidate SuSy genes were identified from the transcriptome sequencing of <em>Stevia rebaudiana</em>, among which SrSUS1 was found to be expressed and active in <em>Escherichia coli</em>. The optimal temperature and pH for SrSUS1 were determined to be 55°C and pH 7.0, respectively. A variant SrSUS1<sub>T49A/L90P/V104E</sub> was generated based on a consensus sequence strategy, exhibiting a 3.6-fold increase in activity and the enhanced affinity for sucrose (<em>K</em><sub>m</sub> = 52.32 mM), as well as the improved thermal stability and catalytic efficiency. By coupling SrSUS1<sub>T49A/L90P/V104E</sub> with glycosyltransferase UGTAn85<sub>Q23E/N65D</sub> or UGT76G4, respectively, the production of 163.32 mM (43.63 g/L) of 2-phenylethyl-β-D-pyranoside and 72.29 mM (93.35 g/L) of rebaudioside M was achieved within 24 h in one-pot, two-enzyme fed-batch reactions. This study provides new insights into plant-derived SuSys and presents a promising biocatalyst for industrial glycosylation applications.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"405 ","pages":"Pages 169-181"},"PeriodicalIF":4.1,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144086101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Production of astaxanthin with high purity and activity based on engineering improvement strategies 基于工程改进策略的高纯度、高活性虾青素的生产

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-05-14 DOI: 10.1016/j.jbiotec.2025.05.012

Chaogang Wang , Zeyu Hong , Mingjian Song , Hao Zheng , Qiaomian Zhou , Haihong Yang , Hui Li , Danqiong Huang

{"title":"Production of astaxanthin with high purity and activity based on engineering improvement strategies","authors":"Chaogang Wang , Zeyu Hong , Mingjian Song , Hao Zheng , Qiaomian Zhou , Haihong Yang , Hui Li , Danqiong Huang","doi":"10.1016/j.jbiotec.2025.05.012","DOIUrl":"10.1016/j.jbiotec.2025.05.012","url":null,"abstract":"<div><div>Here, astaxanthin production in <em>Escherichia coli</em> was systematically improved step by step. By introducing the additional copy of <em>CrtZ</em> and fusion complex of <em>CrtZ</em> and <em>CrtW</em>, astaxanthin content in cells increased from 0.10 mg/g to 0.16 mg/g and 0.63 mg/g DCW, respectively. Remolding the astaxanthin gene cluster by replacing the <em>PanCrtE</em> by <em>HpGGPPS3–1</em> and the fusion of <em>CrtZ</em> and <em>CrtW</em> increased astaxanthin content to 1.98 mg/g DCW. Further selecting the productive host and optimizing culture conditions dramatically increased astaxanthin content to 3.61 mg/g DCW. Subsequently, the fed-batch fermentation achieved the maximum yield of astaxanthin at 509.58 mg/L with the productivity of 7.72 mg/L/h and 5.91 mg/g DCW, covering 98.17 % of detected carotenoids. The chirality analysis assigned the same isomer of astaxanthin extracted from our fermentation system and <em>Haematococcus pluvialis</em>. Moreover, the radical and superoxide anion scavenging activity analysis revealed that astaxanthin achieved in this study performed better than natural astaxanthin extracted from <em>H. pluvialis</em> and chemical synthetic astaxanthin. This study provides a step-by-step example for bioengineering improvement of natural products in <em>E. coli</em> with high purity and activity.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"405 ","pages":"Pages 139-149"},"PeriodicalIF":4.1,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144067929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perspectives on the use of the CRISPR system in plants to improve recombinant therapeutic protein production 利用CRISPR系统在植物中提高重组治疗性蛋白生产的展望

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-05-13 DOI: 10.1016/j.jbiotec.2025.05.010

Edgar Trujillo, Carlos Angulo

{"title":"Perspectives on the use of the CRISPR system in plants to improve recombinant therapeutic protein production","authors":"Edgar Trujillo, Carlos Angulo","doi":"10.1016/j.jbiotec.2025.05.010","DOIUrl":"10.1016/j.jbiotec.2025.05.010","url":null,"abstract":"<div><div>The plant-based system is a promising platform for producing biotherapeutics due to its scalability, cost-effectiveness, and lower risk of contamination by human pathogens. However, several challenges remain, including optimizing yield, stability, functionality, and the immunogenic properties of recombinant proteins. In this context, this review explores the application of CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) technology to improve the production of recombinant therapeutic proteins in plants. Traditional tools and strategies for plant-based recombinant protein production are discussed, highlighting their limitations and the potential of CRISPR to overcome these boundaries. It delves into the components of the CRISPR-Cas system and its application in optimizing therapeutic protein function and yield. Major strategies include modifying glycosylation patterns to humanize plant-produced proteins, metabolic pathway engineering to increase protein accumulation, and the precise integration of transgenes into specific genomic loci to enhance expression stability and productivity. These advancements demonstrate how CRISPR system can overcome bottlenecks in plant molecular farming and enable the production of high-quality therapeutic proteins. Lastly, future trends and perspectives are examined, emphasizing ongoing innovations and challenges in the field. The review underscores the potential of CRISPR to reshape plant biotechnology and support the growing demand for recombinant therapeutics, offering new avenues for sustainable and efficient protein production systems.</div></div><div><h3>Key message</h3><div>CRISPR technology has the potential to improve plant-based therapeutic protein production by optimizing yield, stability, and humanization, overcoming bottlenecks, and enabling sustainable, efficient systems for recombinant biotherapeutics.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"405 ","pages":"Pages 111-123"},"PeriodicalIF":4.1,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144067928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineered L-phenylserine aldolase enhances L-norvaline synthesis within an enzyme cascade 工程l -苯基丝氨酸醛缩酶在酶级联中增强l -正缬氨酸合成

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-05-10 DOI: 10.1016/j.jbiotec.2025.05.005

Meijun Tao , Jing Li , Huaiyuan Zhang , Jiuyu Zhan , Xinye Wang , Kai Zhang , Juan Zhang , Zhibin Feng

{"title":"Engineered L-phenylserine aldolase enhances L-norvaline synthesis within an enzyme cascade","authors":"Meijun Tao , Jing Li , Huaiyuan Zhang , Jiuyu Zhan , Xinye Wang , Kai Zhang , Juan Zhang , Zhibin Feng","doi":"10.1016/j.jbiotec.2025.05.005","DOIUrl":"10.1016/j.jbiotec.2025.05.005","url":null,"abstract":"<div><div>L-Norvaline is a crucial intermediate in the synthesis of antihypertensive agents, and its production via biotechnological methods has garnered significant interest and commercial value in recent years. Here, for the enzymatic cascade synthesis of L-norvaline from propionaldehyde and glycine, an L-phenylserine aldolase gene (designated as ppLPA) from <em>Pseudomonas putida</em> was selected. Following the identification of potential mutation sites via error-prone PCR, coupled with site-directed mutagenesis, a single-site mutant, I18T, was identified, exhibiting a 1.4-fold increase in enzyme activity. Then, the ppLPA mutant I18T was combined with L-threonine deaminase, L-leucine dehydrogenase, and alcohol dehydrogenase to construct a one-pot, multi-enzyme cascade catalytic system for L-norvaline synthesis. The reaction conditions were systematically optimized. To mitigate the inhibitory effects of propionaldehyde, we employed a pH-stat substrate feeding strategy. Under optimal reaction conditions, L-norvaline production achieved a maximum yield of 116.5 g/L after 14 h of reaction, with a conversion rate exceeding 99 % in a 1 L reaction volume. This study highlights significant advancements in improving L-norvaline production, providing potential for more efficient biomanufacturing processes and broader industrial applications.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"405 ","pages":"Pages 99-110"},"PeriodicalIF":4.1,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143942122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hybrid Deep Maxout-VGG-16 model for brain tumour detection and classification using MRI images 基于MRI图像的混合深度Maxout-VGG-16脑肿瘤检测与分类模型。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-05-09 DOI: 10.1016/j.jbiotec.2025.05.009

T. Loganayagi , Meesala Sravani , Balajee Maram , Telu Venkata Madhusudhana Rao

{"title":"Hybrid Deep Maxout-VGG-16 model for brain tumour detection and classification using MRI images","authors":"T. Loganayagi , Meesala Sravani , Balajee Maram , Telu Venkata Madhusudhana Rao","doi":"10.1016/j.jbiotec.2025.05.009","DOIUrl":"10.1016/j.jbiotec.2025.05.009","url":null,"abstract":"<div><div>Brain tumor detection is essential to identify tumors at an early stage, allowing for more effective treatment. The patient's chances of recovery and survival can be improved by early detection. The existing methods for detecting brain tumour have several limitations, including limited accessibility, exposure to radiation, high costs and potential for false negatives. To overcome the issues, a Deep Maxout-Visual Geometry Group-16 (DM-VGG-16) model is devised for detecting tumour in brain from Magnetic Resonance Imaging (MRI). Initially, MRI image is sent for pre-processing as input. Here, Non-Local Mean (NLM) filter performs pre-processing. The pre-processed image is subjected to segmentation stage, which is accomplished by Template–based K-means and improved Fuzzy C Means algorithm (TKFCM). Moreover, in feature extraction stage, various features, like area, cluster prominence, Hybrid PCA- Normalized GIST (NGIST) and Improved Median binary Pattern (IMBP) are extracted. Lastly, proposed DM-VGG-16 model is utilized for detection of brain tumors from extracted features. The DM-VGG-16 is the integration of Deep Maxout Network (DMN) and Visual Geometry Group-16 (VGG-16). The DM-VGG-16 outperformed superior results than conventional techniques with performance metrics, including accuracy, True Negative Rate (TNR) and True Positive Rate (TPR) of 90.76 %, 90.65 % and 90.75 % correspondingly.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"405 ","pages":"Pages 124-138"},"PeriodicalIF":4.1,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143986437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced robustness and fermentation characteristics of lager yeast in high gravity brewing through accumulation of intracellular proline 通过细胞内脯氨酸的积累提高了高重力酿造中大啤酒酵母的健壮性和发酵特性

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-05-08 DOI: 10.1016/j.jbiotec.2025.05.008

Chengtuo Niu , Huating Chen , Jinjing Wang , Chunfeng Liu , Qi Li

{"title":"Enhanced robustness and fermentation characteristics of lager yeast in high gravity brewing through accumulation of intracellular proline","authors":"Chengtuo Niu , Huating Chen , Jinjing Wang , Chunfeng Liu , Qi Li","doi":"10.1016/j.jbiotec.2025.05.008","DOIUrl":"10.1016/j.jbiotec.2025.05.008","url":null,"abstract":"<div><div>In beer production, lager yeasts are subjected to harsh environment in high-gravity brewing (HGB, 24°P or more), thus leading to reduced fermentation performance, increased mortality and formation of off-flavors. This study aimed to improve the vitality, viability and fermentation characteristics of lager yeast during HGB through the accumulation of intracellular proline and to reveal the potential mechanism. A mutant lager yeast Y-100 with significantly increased intracellular proline fluorescence intensity of 37.37 % was obtained by Atmospheric and Room Temperature Plasma (ARTP) mutagenesis. Compared to parental YY, the mutant Y-100 had 13.94 % higher intracellular ATP content, 23.01 % lower ROS accumulation and 77.71 % lower mortality rate at the end of serial batch fermentation for 5 times. Moreover, the time for beer matureness by Y-100 strain was shorted by one day while the real degree of fermentation value was 2.76 % higher using 24°P wort. Through genome resequencing, RT-qPCR analysis and gene knockout and overexpression, the up-regulation of <em>GNP1</em> and <em>SUA7</em> genes in Y-100 strain might contribute to the proline accumulation in lager yeast cells, thus resulting in energy supply and stress protection for lager yeast during HGB. The results not only provided new insights into the role of proline in lager yeast towards unfavorable industrial condition, but also obtained a high-efficient Y-100 strain for potential HGB application.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"405 ","pages":"Pages 26-38"},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Valorization of shopping center side- and waste streams with plant cell culture technology – Prospects and cost reduction 植物细胞培养技术在购物中心边和废物流中的应用前景和降低成本。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-05-08 DOI: 10.1016/j.jbiotec.2025.05.006

Amanda Lillberg, Maria Pajumo, Eevi Haajanen, Terhi Viinikanoja, Anneli Ritala

{"title":"Valorization of shopping center side- and waste streams with plant cell culture technology – Prospects and cost reduction","authors":"Amanda Lillberg, Maria Pajumo, Eevi Haajanen, Terhi Viinikanoja, Anneli Ritala","doi":"10.1016/j.jbiotec.2025.05.006","DOIUrl":"10.1016/j.jbiotec.2025.05.006","url":null,"abstract":"<div><div>Upcycling potential of side-streams and waste material, originating from a shopping center were studied as a feedstock for plant cell cultures of arctic bramble (<em>Rubus arcticus</em> L.), barley (<em>Hordeum vulgare</em> L.) and tobacco BY-2 (<em>Nicotiana tabacum</em> L.). Soft drink waste mix, orange peels, expired bread, spent coffee grounds, and brewer’s spent grain, were used in cultivation experiments to supplement sucrose in the basic growth medium. Artificial urine was included to represent a future scenario, where also human urine could be used as a circular feedstock along improvement in purification technologies allowing removal of microbes and toxic molecules. Diluted artificial urine was used to replace elemental macronutrients e.g., ammonium, phosphate, and sulphate in the culture medium. Soft drink waste mix and artificial urine contributed to the highest biomass accumulation of the tested side streams. Culture medium composition optimization, exploiting combination of soft drink waste mix and artificial urine, was conducted with design of experiments software. The optimized culture medium contributed to notable culture medium cost reductions of 55 %, 33 % and 45 % for arctic bramble, tobacco BY-2 and barley cell lines, respectively, without compromising the biomass yield. However, modified culture medium induced some changes in the amino acid composition of arctic bramble plant cell biomass, e.g., increased asparagine and decreased arginine, alanine and tyrosine contents. Thus, attention should be paid to the amino acid composition in further studies. Our findings confirm the potential of upcycling side streams and replacing costly growth medium components with so far unutilized resources.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"405 ","pages":"Pages 150-158"},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A chitosan-integrated antibacterial protein composite nanocomplex derived from barnacle cement and spider silk 从藤壶水泥和蜘蛛丝中提取的壳聚糖整合抗菌蛋白复合纳米复合物

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-05-08 DOI: 10.1016/j.jbiotec.2025.05.007

Luona Ye, Zitang Xu, Yunchong Li, Pengbo Wang, Yunjun Yan, Jinyong Yan

{"title":"A chitosan-integrated antibacterial protein composite nanocomplex derived from barnacle cement and spider silk","authors":"Luona Ye, Zitang Xu, Yunchong Li, Pengbo Wang, Yunjun Yan, Jinyong Yan","doi":"10.1016/j.jbiotec.2025.05.007","DOIUrl":"10.1016/j.jbiotec.2025.05.007","url":null,"abstract":"<div><div>While barnacle cement protein cp19k (from <em>Megabalanus rosa</em>) possesses remarkable adhesion properties and spider silk protein MaSp1 (from <em>Nephila clavata</em> dragline silk) demonstrates exceptional toughness, their advancements in medical biomaterials are significantly hindered by their limitations in antimicrobial properties. In this study, composite nanocomplexes incorporating chitosan and proteins derived from barnacle cement and spider silk were designed and biofabricated for enhanced antibacterial properties. The impact of chitosan’s molecular weight on the properties of nanocomplexes comprising cp19k-MaSp1/chitosan, MaSp1/chitosan, and cp19k/chitosan was evaluated. The results revealed that low molecular weight chitosan (LMWC, Mw = 1 kDa) forms nanocomplexes that exhibit distinct structural differences in comparison to those formed with high molecular weight chitosan (HMWC, Mw ≥ 150 kDa). Furthermore, cp19k-MaSp1/C<sub>150k</sub> exhibited the most potent antibacterial activity against <em>E. coli</em> and <em>S. aureus</em>, surpassing the performance of cp19k, MaSp1, cp19k-MaSp1, and chitosan individually, achieving inhibition by disrupting the bacterial cell membrane structure and elevating the intracellular ROS level. Meanwhile, On day 6, the viability of HUVECs (Human Umbilical Vein Endothelial Cells) of cp19k-MaSp1/C<sub>150k</sub> had attained a level of 145.21 ± 6.23 %, representing a substantial elevation when compared to C<sub>150k</sub>. The remarkable biocompatibility of nanocomplexes cp19k-MaSp1/C<sub>150k</sub> holds potential for application in wound dressings and tissue repair.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"405 ","pages":"Pages 57-71"},"PeriodicalIF":4.1,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143931888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0