Journal of biotechnology最新文献

{"title":"Production and accumulation of recombinant protein via vitellogenin transport pathway in Daphnia magna.","authors":"Yusuke Tsuji, Fransiscus Jason Wiguna, Nikko Adhitama, Pijar Religia, Yasuhiko Kato, Hajime Watanabe","doi":"10.1016/j.jbiotec.2026.04.015","DOIUrl":"https://doi.org/10.1016/j.jbiotec.2026.04.015","url":null,"abstract":"<p><p>The demand for recombinant proteins is rising and various systems for protein production have been developed, including cultured cells, yeast, and bacteria. This study explores the use of Daphnia, a small crustacean, as a host for recombinant protein production. Daphnia can be cultured easily and inexpensively. We aimed to accumulate recombinant proteins in its eggs. This research aimed to identify the signal peptides necessary for transporting vitellogenin into Daphnia eggs and to develop a system for transporting recombinant proteins into the eggs using these signals. A fusion protein of GFP (Green Fluorescent Protein) with a predicted transport signal sequence was expressed in D. magna, and we confirmed that GFP is secreted into the hemolymph. Additionally, a specific region of about 300 amino acids, presumed necessary for binding to vitellogenin receptors, was added to the fusion GFP and expressed outside the ovaries, resulting in strong GFP fluorescence in the eggs. These results indicate that by fusing signals for protein secretion and those that enable uptake into eggs, recombinant protein can be encapsulated into the eggs. This system shows promise as a simple and cost-effective method for producing recombinant proteins by accumulating them in Daphnia eggs.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strengthening Carbon Concentrating Mechanisms of Ambient CO₂ with OLA@KAUST-7 Nanoparticles for Producing Microalgal Biomass.","authors":"Wanlin Liu, Ruhan Guo, Ying Liu, Dongwei Jia, Yuxiang Mao, Xuecheng Wu, Junhu Zhou, Jun Cheng","doi":"10.1016/j.jbiotec.2026.04.021","DOIUrl":"https://doi.org/10.1016/j.jbiotec.2026.04.021","url":null,"abstract":"<p><p>In response to the mass transfer limitation of low concentration CO₂ (approximately 400 ppm) in closed tubular photobioreactors aimed at improving indoor air quality, nanomaterials with excellent hydrolytic stability, OLA@KAUST-7, served as CO₂ carriers. This approach sought to enhance carbon sequestration efficiency and biomass accumulation in Chlorella. The experiment examined the effects of varying addition amounts (0.01, 0.05, and 0.1mmol/L) on the growth and physiological metabolism of Chlorella. The results indicated that 0.01mmol/L represented the optimal dosage; at this concentration, the conversion efficiency of algal liquid CO₂ to HCO₃^- increased by 55.7%. Transcriptomic analysis confirmed that the introduction of an appropriate amount of OLA@KAUST-7 significantly upregulated the gene expression of key proteins in the photosystem, such as Lhcb and psbO, as well as core energy metabolism enzymes like gpi and aco. This upregulation synergistically enhanced photochemical energy conversion efficiency and intracellular energy supply. The synthesis capacity of photosynthetic pigments in Chlorella significantly improved, ultimately resulting in a 53.06% increase in biomass yield.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838420","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leveraging Mixed-Mode Filter During Cell Culture Harvest to Prevent Antibody Reduction.","authors":"Zhenshu Wang, Michael Hartmann, Geetanjali Pendyala, Vivian Gasca, Andrew Hsieh, Majd Alslaiti, Shannon Rivera, Anton Kozyryev, Suman Nandy, Ruilin Qian, Suyang Wu, Xiaolin Zhang, Patricia Rose, Sandra Bennun, Sanjeev Ahuja","doi":"10.1016/j.jbiotec.2026.05.001","DOIUrl":"https://doi.org/10.1016/j.jbiotec.2026.05.001","url":null,"abstract":"<p><p>Disulfide bond reduction was observed for a monoclonal antibody (mAb1) produced in a Chinese Hamster Ovary (CHO) process, driven primarily by the thioredoxin (Trx)-thioredoxin reductase (TrxR) system present in harvested cell culture fluid (HCCF). To address this issue, we evaluated the Solventum Polisher ST (PST) depth filter as a secondary harvest-stage filtration step designed to remove redox-active enzymes and improve product stability. PST filtration reduced Trx and glucose-6-phosphate 1-dehydrogenase (G6PD) by approximately 1log at loadings up to 150L/m² and non-detectable TrxR at loadings up to 100L/m², as demonstrated by mass spectrometry. Protein biophysical property calculations indicated strong electrostatic attraction between Trx and mAb1 that may facilitate enzyme-antibody co-elution through conventional quaternary amine filters. Compared with dilution, KMnO₄ oxidation, and reversible inhibition using L-cystine, PST filtration most effectively suppressed disulfide bond reduction over seven days at room temperature. Pre-spiking HCCF with guanidinium-containing compounds prior to PST further extended mAb1 stability in the 100-200L/m² fraction from 19.5h to 72.5-95.3h, confirming the functional role of guanidinium groups in mitigating reduction. Scalability up to 50L pilot batch assessments supported consistent performance at larger volumes, demonstrating PST filtration as a practical and robust approach for controlling enzymatic reduction during bioprocess harvest operations.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":""},"PeriodicalIF":3.9,"publicationDate":"2026-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147838423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Fenugreek gum-based microalgae harvesting technology and its mechanism of action","authors":"Xichen Zheng,&nbsp;Hao Wen,&nbsp;Kemin Wei,&nbsp;Jia He,&nbsp;Manli Wang,&nbsp;Meili Wu","doi":"10.1016/j.jbiotec.2026.02.002","DOIUrl":"10.1016/j.jbiotec.2026.02.002","url":null,"abstract":"<div><div>The use of microalgae to produce renewable biomass is a promising approach to addressing environmental and energy challenges, but the high cost of microalgae harvesting limits its industrial application. To explore the efficient and low-cost harvesting of microalgae, a novel method utilizing fenugreek gum for bubble-free harvesting was developed. Single-factor experiments and response surface analysis were used to screen the significant influencing factors. A multi-objective optimization model was established to determine the key factors influencing harvesting efficiency, including pH, sodium cellulose addition, stirring time, stirring speed, flotation material addition, and raw algal volume. This resulted in an optimal harvesting efficiency of 94.026 % and an optimal enrichment ratio of 2.5 %. Based on the life cycle evaluation, it was concluded that the environmental impact of fenugreek gum on climate change was 1.14 kg CO₂ eq, and the production cost was $3.15, which was significantly lower than the traditional harvesting method. Mechanistic Analysis indicates that the adhesion of fenugreek gum to microalgae is achieved through an electrostatic neutralization and adsorption bridging mechanism.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"412 ","pages":"Pages 14-25"},"PeriodicalIF":3.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146142549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yarrowia lipolytica, Komagataella phaffii and secretory proteins: Recombinant laccase as a case study","authors":"Constance Keller ,&nbsp;Rocio Cozmar ,&nbsp;Miguel Alcalde ,&nbsp;Patrick Fickers","doi":"10.1016/j.jbiotec.2026.01.015","DOIUrl":"10.1016/j.jbiotec.2026.01.015","url":null,"abstract":"<div><div>For decades, <em>Komagataella phaffii</em> has been the reference host for recombinant secretory protein (rsProt) production. However, secretion bottlenecks associated with the limited processing capacity of the endoplasmic reticulum restrict the secretion efficiency under high protein loads. Besides, <em>Yarrowia lipolytica</em> possesses a secretion pathway resembling that of filamentous fungi and naturally secretes large amounts of hydrolytic enzymes, making it a promising alternative host. In this study, we systematically investigated the impact of pre- and pro-sequences on the secretion of LacVader, an evolved laccase with industrial applications. In <em>Y. lipolytica</em>, nine constructs combining four pre-sequences (αPre, Yps3Pre, Lip2Pre, SoAmyPre) and two pro-sequences (αPro, Lip2Pro) were integrated at the <em>LIP2</em> locus and expressed under the constitutive P_TEF promoter. Comparative analysis revealed that most pre–pro constructs resulted in higher laccase specific activity compared to <em>K. phaffii</em> expressing the enzyme under the canonical P_AOX1 promoter and the <em>S. cerevisiae</em> α-factor signal peptide. Notably, both the pre- and pro-sequences had a strong influence on laccase secretion in <em>Y. lipolytica</em>. The Lip2Pro sequence consistently enhanced secretion, with the Yps3Pre–Lip2Pro construct yielding the highest activity, eightfold greater than that obtained in <em>K. phaffii</em>. These findings highlight the crucial role of secretion signal optimization in rsProt production and confirm the superior potential of <em>Y. lipolytica</em> over <em>K. phaffii</em> as a robust host for industrial enzyme secretion.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"412 ","pages":"Pages 1-4"},"PeriodicalIF":3.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146097119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and functional analysis of anti-human PD-1 monoclonal antibody in transgenic plants","authors":"Chae Eun Lee ,&nbsp;Sohee Lim ,&nbsp;Da Won Lee ,&nbsp;Jong Seok Lim ,&nbsp;Jin Wook Kim ,&nbsp;Soon Auck Hong ,&nbsp;Hye Jun Lee ,&nbsp;Kisung Ko ,&nbsp;Soon Chul Myung","doi":"10.1016/j.jbiotec.2026.01.016","DOIUrl":"10.1016/j.jbiotec.2026.01.016","url":null,"abstract":"<div><div>Plant-based biopharmaceutical platforms offer a cost-effective and scalable alternative for therapeutic antibody production. In this study, transgenic <em>Nicotiana tabacum</em> (<em>N. tabacum</em>) plants were generated to express pembrolizumab, an anti-human programmed cell death protein-1 (PD-1) monoclonal antibody (mAb), targeting both classical PD-1 on immune cells and the recently identified intrinsic PD-1 (iPD-1) variant in tumor cells. The plant-derived anti-PD-1 mAb (mAb^P PD-1) was successfully purified and validated through SDS-PAGE and immunoblotting. Functional analyses using ELISA and immunohistochemistry demonstrated that mAb^P PD-1 exhibits strong binding affinity to recombinant human PD-1 and efficiently detects PD-1 expression in human tonsil tissue. Importantly, cell-based assays demonstrated that mAb^P PD-1 binds effectively to iPD-1-expressing bladder urothelial cancer cell lines, resulting in significant inhibition of cell proliferation. Mechanistically, Western blot analysis revealed that mAb^P PD-1 markedly suppresses extracellular signal-regulated kinase (ERK) phosphorylation without altering total ERK levels, indicating direct modulation of the mitogen-activated protein kinase (MAPK) signaling pathway associated with tumor cell proliferation. These findings establish transgenic tobacco plants as a cost-effective and scalable platform for producing functional anti-PD-1 antibodies with potent immunoregulatory and anti-proliferative properties. The dual targeting of immune cell PD-1 and tumor cell iPD-1 underscores the therapeutic potential of plant-derived antibodies in cancer immunotherapy.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"412 ","pages":"Pages 5-13"},"PeriodicalIF":3.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative transcriptomic and genome wide analysis reveals class III peroxidase responses to abiotic stresses in Selenicereus undatus","authors":"Aamir Ali Khokhar ,&nbsp;Zhang You ,&nbsp;Liu Hui ,&nbsp;Darya Khan ,&nbsp;Qamar U Zaman ,&nbsp;Babar Usman ,&nbsp;Hua-Feng Wang","doi":"10.1016/j.jbiotec.2026.02.001","DOIUrl":"10.1016/j.jbiotec.2026.02.001","url":null,"abstract":"<div><h3>Background</h3><div><em>Selenicereus undatus</em> (<em>S.undatus</em>) is an epiphyte cacti and it is largely grown in Asia. Drought, salinity and heavy metal stress are the major restricting factors in its growth and productivity in nature. Class III peroxidases (PODs) are important genes in response of plants to oxidative and abiotic stresses, as was found by a number of studies. Nevertheless, the genomic structure and functional analyses of POD genes in S. undatus have not been well investigated.</div></div><div><h3>Results</h3><div>In this study, the physiological and molecular responses of <em>Selenicereus undatus</em> to single and multi-factorial environmental stresses were investigated in comparison with melatonin supplementation. However, stress significantly increased the activities of peroxidase (POD), proline content and hydrogen peroxide (H₂O₂) accumulation, which were strongly attenuated by the application of melatonin through maintaining an equilibrium in redox status to promote growth. Transcriptome analysis observed obvious differential expression in the <em>HuPOD</em> gene family when under different stress treatments. In the <em>S. undatus</em> genome 75 <em>HuPOD</em> genes were identified and classified into five phylogenetic subgroups. Among them, <em>HuPOD-02/05/06/12/13/29</em> were strongly up-regulated under combined stress while <em>HuPOD-36/56</em> showed slight downregulation to single stresses conditions. KEGG enrichment analysis revealed that under single and multi-factorial stresses, differentially expressed <em>HuPOD</em> genes were significantly enriched in the MAPK signaling pathway, glutathione metabolism, and phenylpropanoid biosynthesis, suggesting pivotal roles in oxidative stress regulation and signal transduction in S. undatus. RT-qPCR validation confirmed RNA-seq expression patterns for five representative genes (<em>HuPOD-01/10/36/56/62</em>), with <em>HuPOD-10/62</em> showing the highest induction under melatonin-supplemented stresses (Cd+S+D+M).</div></div><div><h3>Conclusion</h3><div>These findings suggested that POD gene family may participate in the response to single and multifactorial stress in <em>S.undatus</em> This study provides a scientific basis for the further development and functional validation of the POD gene family in <em>S.undatus.</em></div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"412 ","pages":"Pages 26-43"},"PeriodicalIF":3.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146165480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic regulation of ROS stress in the accumulation of L-lysine in Escherichia coli","authors":"Li Zhang,&nbsp;Jianan Yang,&nbsp;Zhixuan Lv,&nbsp;Longjun Han,&nbsp;Zhiwei Zha,&nbsp;Zhijie Cheng,&nbsp;Xu Yang,&nbsp;Zhengyang Xu,&nbsp;Lei Yang,&nbsp;Jian Gao","doi":"10.1016/j.jbiotec.2026.01.011","DOIUrl":"10.1016/j.jbiotec.2026.01.011","url":null,"abstract":"<div><div>Oxidative stress in <em>Escherichia coli</em> during chemical production, caused by reactive oxygen species (ROS), impairs cell viability and limits output. This study addressed product accumulation and ROS stress in <span>L</span>-lysine-producing strains. Genetic engineering created three variants: dynamically up-regulated R1, down-regulated R2, and temporally regulatable R3 to reduce ROS during fermentation. Under stress conditions, all engineered strains showed significantly lower ROS levels versus parental strain R0, with R3 exhibiting the greatest reduction. Fermentation confirmed R3's superior performance, yielding 85.8 % more <span>L</span>-lysine than R0. These results demonstrate that constructing ROS mitigator achieves precise ROS control and efficient <span>L</span>-lysine synthesis. This dynamic regulation strategy enhances cell viability and production performance under oxidative stress, providing a viable approach for improving engineered cell factories' stress resistance.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"411 ","pages":"Pages 66-77"},"PeriodicalIF":3.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146052160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative omics approaches for bioactive metabolite discovery in marine macroalgae: Recent advances and future perspectives","authors":"M. Marimuthu ,&nbsp;M. Anitha ,&nbsp;P. Prakash ,&nbsp;Meivelu Moovendhan","doi":"10.1016/j.jbiotec.2026.01.007","DOIUrl":"10.1016/j.jbiotec.2026.01.007","url":null,"abstract":"<div><div>Phlorotannins, bromophenols, sulfated polysaccharides, terpenoids, lipids and halogenated molecules exhibit potent antioxidant, anticancer, anti-inflammatory, and antimicrobial properties. Conventional discovery methods, such as solvent extraction and bioassay-guided fractionation, are limited by low throughput, specificity, and poor insight into biosynthetic mechanisms. High-throughput omics platforms including genomics, transcriptomics, proteomics, and metabolomics have transformed the discovery of bioactive metabolites in macroalgae by unraveling system-wide interpretation of biosynthetic pathways. This review outlines omics-driven strategies for macroalgal natural product research, highlighting genome sequencing and annotation efforts. Transcriptome-based biosynthetic gene cluster (BGC) mining, and gene regulation analysis via RNA-seq. Proteomic approaches such as 2D electrophoresis, LC-MS/MS, and MALDI-TOF are discussed for their role in elucidating enzyme functions and post-translational modifications. Metabolomics tools, including GC-MS, LC-MS, and NMR, paired with platforms like GNPS and MetaboAnalyst, have improved metabolite identification, dereplication and pathway analysis. Emerging integrative multi-omics frameworks combining machine learning and systems biology are paving the way for predictive, precision-driven bioprospecting. Nonetheless, challenges persist, including incomplete genome assemblies, taxonomic inconsistencies and limited reference databases. This review emphasizes how omics technologies are deciphering and mechanistically elucidating the untapped metabolic potential of marine macroalgae, accelerating their application in biotechnology and therapeutic innovation.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"411 ","pages":"Pages 13-28"},"PeriodicalIF":3.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two industrial media reveal a mitochondrial disfunction in CHO cell cultures co-fed with glucose and lactic acid","authors":"Keegan Orzechowski ,&nbsp;Johanna Vappiani-Korben ,&nbsp;Daniel C. Sevin ,&nbsp;Juan Aon","doi":"10.1016/j.jbiotec.2026.01.002","DOIUrl":"10.1016/j.jbiotec.2026.01.002","url":null,"abstract":"<div><div>Metabolomics analyses of cell culture processes can provide valuable insight into cellular physiology that can be leveraged to develop more productive processes. In this work, we applied metabolomics to interrogate CHO cell behavior in two industrial chemically-defined media in cultures co-fed with glucose and lactic acid. We previously reported that secreted acylcarnitines are indicative of altered mitochondrial metabolism when cultures are fed lactic acid and serve to maintain homeostasis between free CoA, acetyl-CoA, free carnitines, and acylcarnitines (Vappiani <em>et al</em>., 2021). One of the two media (“Medium B”) increased significantly viable-cell count and antibody titer than Medium A. Here, we report that CHO’s mitochondrial dysfunctionality based on the secretion of acylcarnitines in lactic acid-fed cultures depends on the overall medium composition. We hypothesize that in order to achieve better growth and titer, Medium B exhibited an increased oxidative phosphorylation based on the lower secretion of acylcarnitines and a differential utilization of riboflavin and thiamine, precursors of coenzymes required to enhance mitochondrial pyruvate incorporation and TCA cycle function. Therefore, our data provides further evidence that non-obvious changes to medium composition can have substantial effects on CHO-based production processes by altering the activity of oxidative phosphorylation required for the proper functioning of mitochondria but also for better antibody production.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"411 ","pages":"Pages 1-11"},"PeriodicalIF":3.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145933438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}