Journal of biotechnology最新文献

{"title":"Mixed-Carbon Fermentation with Hydrocarbons and Fatty Acids for Enhanced Medium-Chain-Length Polyhydroxyalkanoate Production.","authors":"Krishanthi Bandara, Masoumeh Mohandessi, Xianglan Bai, Xiangwei Du, Caixia Wan","doi":"10.1016/j.jbiotec.2026.10.014","DOIUrl":"https://doi.org/10.1016/j.jbiotec.2026.10.014","url":null,"abstract":"<p><p>Mixed-carbon streams containing fatty acids and hydrocarbons are relevant to polyhydroxyalkanoate (PHA) biomanufacturing, particularly for valorizing waste and upcycling plastics that yield chemically diverse substrates. This study evaluated fatty acids (C6-C18), hydrocarbons (C6-C18), and their mixtures for medium-chain-length polyhydroxyalkanoate (mcl-PHA) production by Pseudomonas putida (PP), Pseudomonas resinovorans (PR), and a PP-PR co-culture. Among individual fatty acids, decanoic acid (C10) resulted in the highest cell concentration (2.05g/L). Medium-chain-rich fatty-acid mixtures (C6-C12, with or without C14) promoted PHA accumulation, whereas C8-containing hydrocarbon feeds favored growth. The PP-PR co-culture grown on the mixed hydrocarbon-fatty-acid formulation HCFA-1 achieved the highest PHA content among the tested configurations (60.96wt%), with a titer of 2.11g/L. The polymers consisted predominantly of (R)-3-hydroxyoctanoic acid (HO) and (R)-3-hydroxydecanoic acid (HD), with (R)-3-hydroxydodecanoic acid (HDD) as a secondary component and variable amounts of (R)-3-hydroxyhexanoic acid (HHx) and (R)-3-hydroxytetradecanoic acid (HTD). C8-rich substrate mixtures favored HO, whereas C10-rich mixtures increased the HD fraction. Thus, feed composition can tailor polymer composition in addition to supporting microbial growth and PHA accumulation. Because HO and HD differ in side-chain length, these compositional shifts may affect chain packing and crystallization. Adjusting the HO:HD ratio could therefore help tailor flexibility and thermal behavior for packaging films, coatings, and other value-added bioplastics. Although PHA titers were modest, this study examined substrate-composition effects rather than high-titer process optimization. These findings link mixed-carbon utilization to tunable mcl-PHA composition and provide guidance for designing feed formulations for targeted bioplastic applications.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149206895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyscias filicifolia cell suspension culture enriched in triterpene glycosides: Growth parameters and target metabolite profile comparison of long-term in flasks batch culture and in a semi-continuous mode in bioreactor.","authors":"Maria V Titova, Olga N Prudnikova, Tatiana M Tyurina, Artem A Fomenkov, Igor M Ivanov, Alexander V Nosov","doi":"10.1016/j.jbiotec.2026.10.013","DOIUrl":"10.1016/j.jbiotec.2026.10.013","url":null,"abstract":"<p><p>The modern pharma, food and other industries related to serving human needs are constantly demanding for biologically active substances (BAS) of plant origin. Plant cell suspension cultures (CSCs) have long been considered a replacement for depleting traditional BAS sources-wild, cultivated, and agricultural plants-and hold great promise for biotechnological applications. In particular, Polyscias filicifolia plants, which are valuable sources of oleanane-type glycosides with a promising spectrum of pharmacological activity, should be considered as starting material for the establishment of CSCs. There are limited data on long-term cultivation of Polyscias spp. cell cultures and no data on long-term cultivation of P. filicifolia cell cultures with high productivity of oleanane glycosides. This study presents the research of unique seven-year-old CSC of P. filicifolia, namely the Pf-SH strain. The Pf-SH strain was capable of synthesizing a wide range of oleanane glycosides, and their maximum total content (21.4 mg/g DW) was higher than that in whole plant leaves (13.5 mg/g DW). The oleanane glycoside profiles in P. filicifolia leaves and Pf-SH cells were radically different, with a significant predominance of more glycosylated forms (e.g., polyscioside E) in Pf-SH cells. During five years of Pf-SH cells cultivation in flasks, they retained the ability to synthesize eleven oleanane glycosides, and their ratio remained fairly stable. Changes in the content of glycosides and growth parameters of Pf-SH cells were detected during a 372-day semi-continuous cultivation in a bubble-type bioreactor. The optimal strategy for bioreactor cultivation for maximum accumulation of cellular biomass and target BAS is discussed.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":"39-51"},"PeriodicalIF":4.0,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149194162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolic engineering of Klebsiella oxytoca for highly selective meso-2,3-butanediol production with minimized byproducts.","authors":"Chan Woo Song, Jyotiranjan Bal, Jong Myoung Park","doi":"10.1016/j.jbiotec.2026.10.012","DOIUrl":"10.1016/j.jbiotec.2026.10.012","url":null,"abstract":"<p><p>Producing enantiopure meso-2,3-butanediol (meso-2,3-BDO) with minimal organic acid byproducts is critical for its commercial applications in specialized polymers and cosmetics. In this study, we developed a highly selective and robust Klebsiella oxytoca platform using stepwise metabolic engineering and bioprocess optimization. Starting with a lactate- and formate-deficient parent strain (Δldh/ΔpflB), we sequentially deleted poxB and frdBC to suppress acetate and succinate byproducts. To resolve recalcitrant succinate accumulation caused by NADH surplus during micro-aerobic fed-batch fermentation, we implemented dynamic redox control strategies (nutrient supplementation and agitation shifts) and permanently sealed the reductive TCA branch by deleting mdh. Furthermore, to achieve ultra-high stereoisomeric purity, we disrupted the isomer interconversion network by knocking out dar and gldA. The resulting engineered strain (GSC-P8) was cultivated using the optimized redox-control bioprocess. This integrated scale-up fermentation completely abolished undesired stereoisomers, strictly maintaining meso-2,3-BDO selectivity at approximately 97.9%. We achieved a prominent meso-2,3-BDO titer of 125.2 g/L and a volumetric productivity of 2.84 g/L/h, while restricting each acidic byproduct to below 2.0 g/L. This study demonstrates a commercially viable strategy for pure meso-2,3-BDO production through rigorous intracellular redox homeostasis.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":"29-38"},"PeriodicalIF":4.0,"publicationDate":"2026-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149194167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Iron sulfur mineral-based microbial fuel cells: functional orientation, performance-affecting factors, and sustainable development prospects.","authors":"Li Wang, Xiaojun Ma, Yunhao Li, Weihong Zhang, Jun Dong","doi":"10.1016/j.jbiotec.2026.10.009","DOIUrl":"https://doi.org/10.1016/j.jbiotec.2026.10.009","url":null,"abstract":"<p><p>Microbial fuel cells (MFCs) directly convert chemical energy into electrical energy via the microbial catalytic oxidation of organic substrates, demonstrating immense potential for simultaneous pollutant degradation and bioenergy recovery. However, their industrialization has long been hindered by bottlenecks like low electron transfer and energy conversion efficiencies, high material costs, and poor large-scale stability. Iron-sulfur minerals, possessing unique electron transfer properties and environmental compatibility, provide an effective solution for the sustainable development of MFCs. Nevertheless, current literature predominantly focuses on their abiotic catalytic oxidation processes, lacking a systematic review of their applications in MFCs. This partially neglects the complex interactions among intrinsic mineral properties, environmental factors, and interfacial microbial dynamics, limiting an in-depth understanding of system efficacy and engineering bottlenecks. Therefore, this paper systematically reviews the research progress on the applications of iron-sulfur minerals in MFCs, elucidating their core mechanisms of action under diverse application forms. Furthermore, it summarizes key factors influencing the power generation performance and pollutant removal efficiency of the system. Finally, this review analyzes the engineering challenges and proposes future research directions, aiming to provide a fundamental reference for promoting both the technical optimization and practical application of iron-sulfur mineral-based MFCs.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149168127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression of a conserved synthetic multiepitopic malaria protein CS712 in duckweed: immune response evaluation after oral delivery in BALB/c mice.","authors":"Nima Rad, Sedigheh Zakeri, Akram Abouie Mehrizi, Sadegh Shojaei Baghini, Mahdi Arezoumandi, Elham Taghipour, Fateme Frootan, Mahyat Jafari, Ali Hatef Salmanian","doi":"10.1016/j.jbiotec.2026.10.003","DOIUrl":"https://doi.org/10.1016/j.jbiotec.2026.10.003","url":null,"abstract":"<p><strong>Background: </strong>Malaria remains a significant global health challenge, with hundreds of millions of cases reported annually and limited access to effective and affordable vaccines. Owing to their scalability, safety, and potential for oral administration, plant-based vaccine platforms have emerged as promising alternatives. In this study, we evaluated the immunogenicity of a conserved, synthetic, and multiepitopic chimeric circumsporozoite protein, CS712, derived from Plasmodium vivax and expressed in transgenic Lemna minor, as a candidate antigen for oral malaria vaccine development.</p><p><strong>Methods: </strong>The synthetic CS712 gene was cloned into a plant expression vector and introduced into duckweed via Agrobacterium-mediated transformation. Transgenic lines were verified by PCR, and target protein expression was confirmed by Western blot analysis, reaching approximately 0.5% of total soluble protein (TSP) in line L3. BALB/c mice were immunized via three strategies: edible administration, subcutaneous injection, and a prime-boost combination. Immune responses were analyzed by measuring serum IgG, serum IgA, fecal secretory IgA, IgG subclasses, and cytokines, including IFN-γ and IL-5. Splenocyte proliferation was also assessed by flow cytometric cell-cycle analysis.</p><p><strong>Results: </strong>CS712-expressing duckweed induced both systemic and mucosal immune responses in immunized mice. Edible and prime-boost immunization increased serum IgG and IgA levels as well as fecal secretory IgA responses compared with the non-transgenic control. Notably, serum IgA and fecal s-IgA levels were higher in the edible and prime-boost groups than in the injection group, indicating enhanced IgA-associated responses following oral antigen delivery. IgG subclass analysis and cytokine profiling showed route-dependent immune patterns. The injection group showed the strongest IgG1-dominated and absolute cytokine responses, whereas the edible/oral group showed the highest IgG2a/IgG1 and IFN-γ/IL-5 ratios, suggesting a relatively higher Th1-associated immune balance.</p><p><strong>Conclusions: </strong>These findings support the feasibility of using transgenic duckweed as a plant-based platform for producing a multiepitopic malaria vaccine candidate. CS712-expressing duckweed induced systemic antibody responses, mucosal IgA responses, splenocyte proliferation, and cytokine production, supporting its immunogenicity after oral antigen delivery. However, while this study demonstrates immunogenicity, further studies involving dose optimisation, formulation, and challenge experiments are required to determine protective efficacy.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":" ","pages":""},"PeriodicalIF":4.0,"publicationDate":"2026-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"149127249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of Fenugreek gum-based microalgae harvesting technology and its mechanism of action","authors":"Xichen Zheng,&nbsp;Hao Wen,&nbsp;Kemin Wei,&nbsp;Jia He,&nbsp;Manli Wang,&nbsp;Meili Wu","doi":"10.1016/j.jbiotec.2026.02.002","DOIUrl":"10.1016/j.jbiotec.2026.02.002","url":null,"abstract":"<div><div>The use of microalgae to produce renewable biomass is a promising approach to addressing environmental and energy challenges, but the high cost of microalgae harvesting limits its industrial application. To explore the efficient and low-cost harvesting of microalgae, a novel method utilizing fenugreek gum for bubble-free harvesting was developed. Single-factor experiments and response surface analysis were used to screen the significant influencing factors. A multi-objective optimization model was established to determine the key factors influencing harvesting efficiency, including pH, sodium cellulose addition, stirring time, stirring speed, flotation material addition, and raw algal volume. This resulted in an optimal harvesting efficiency of 94.026 % and an optimal enrichment ratio of 2.5 %. Based on the life cycle evaluation, it was concluded that the environmental impact of fenugreek gum on climate change was 1.14 kg CO₂ eq, and the production cost was $3.15, which was significantly lower than the traditional harvesting method. Mechanistic Analysis indicates that the adhesion of fenugreek gum to microalgae is achieved through an electrostatic neutralization and adsorption bridging mechanism.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"412 ","pages":"Pages 14-25"},"PeriodicalIF":3.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146142549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Yarrowia lipolytica, Komagataella phaffii and secretory proteins: Recombinant laccase as a case study","authors":"Constance Keller ,&nbsp;Rocio Cozmar ,&nbsp;Miguel Alcalde ,&nbsp;Patrick Fickers","doi":"10.1016/j.jbiotec.2026.01.015","DOIUrl":"10.1016/j.jbiotec.2026.01.015","url":null,"abstract":"<div><div>For decades, <em>Komagataella phaffii</em> has been the reference host for recombinant secretory protein (rsProt) production. However, secretion bottlenecks associated with the limited processing capacity of the endoplasmic reticulum restrict the secretion efficiency under high protein loads. Besides, <em>Yarrowia lipolytica</em> possesses a secretion pathway resembling that of filamentous fungi and naturally secretes large amounts of hydrolytic enzymes, making it a promising alternative host. In this study, we systematically investigated the impact of pre- and pro-sequences on the secretion of LacVader, an evolved laccase with industrial applications. In <em>Y. lipolytica</em>, nine constructs combining four pre-sequences (αPre, Yps3Pre, Lip2Pre, SoAmyPre) and two pro-sequences (αPro, Lip2Pro) were integrated at the <em>LIP2</em> locus and expressed under the constitutive P_TEF promoter. Comparative analysis revealed that most pre–pro constructs resulted in higher laccase specific activity compared to <em>K. phaffii</em> expressing the enzyme under the canonical P_AOX1 promoter and the <em>S. cerevisiae</em> α-factor signal peptide. Notably, both the pre- and pro-sequences had a strong influence on laccase secretion in <em>Y. lipolytica</em>. The Lip2Pro sequence consistently enhanced secretion, with the Yps3Pre–Lip2Pro construct yielding the highest activity, eightfold greater than that obtained in <em>K. phaffii</em>. These findings highlight the crucial role of secretion signal optimization in rsProt production and confirm the superior potential of <em>Y. lipolytica</em> over <em>K. phaffii</em> as a robust host for industrial enzyme secretion.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"412 ","pages":"Pages 1-4"},"PeriodicalIF":3.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146097119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expression and functional analysis of anti-human PD-1 monoclonal antibody in transgenic plants","authors":"Chae Eun Lee ,&nbsp;Sohee Lim ,&nbsp;Da Won Lee ,&nbsp;Jong Seok Lim ,&nbsp;Jin Wook Kim ,&nbsp;Soon Auck Hong ,&nbsp;Hye Jun Lee ,&nbsp;Kisung Ko ,&nbsp;Soon Chul Myung","doi":"10.1016/j.jbiotec.2026.01.016","DOIUrl":"10.1016/j.jbiotec.2026.01.016","url":null,"abstract":"<div><div>Plant-based biopharmaceutical platforms offer a cost-effective and scalable alternative for therapeutic antibody production. In this study, transgenic <em>Nicotiana tabacum</em> (<em>N. tabacum</em>) plants were generated to express pembrolizumab, an anti-human programmed cell death protein-1 (PD-1) monoclonal antibody (mAb), targeting both classical PD-1 on immune cells and the recently identified intrinsic PD-1 (iPD-1) variant in tumor cells. The plant-derived anti-PD-1 mAb (mAb^P PD-1) was successfully purified and validated through SDS-PAGE and immunoblotting. Functional analyses using ELISA and immunohistochemistry demonstrated that mAb^P PD-1 exhibits strong binding affinity to recombinant human PD-1 and efficiently detects PD-1 expression in human tonsil tissue. Importantly, cell-based assays demonstrated that mAb^P PD-1 binds effectively to iPD-1-expressing bladder urothelial cancer cell lines, resulting in significant inhibition of cell proliferation. Mechanistically, Western blot analysis revealed that mAb^P PD-1 markedly suppresses extracellular signal-regulated kinase (ERK) phosphorylation without altering total ERK levels, indicating direct modulation of the mitogen-activated protein kinase (MAPK) signaling pathway associated with tumor cell proliferation. These findings establish transgenic tobacco plants as a cost-effective and scalable platform for producing functional anti-PD-1 antibodies with potent immunoregulatory and anti-proliferative properties. The dual targeting of immune cell PD-1 and tumor cell iPD-1 underscores the therapeutic potential of plant-derived antibodies in cancer immunotherapy.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"412 ","pages":"Pages 5-13"},"PeriodicalIF":3.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146100262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative transcriptomic and genome wide analysis reveals class III peroxidase responses to abiotic stresses in Selenicereus undatus","authors":"Aamir Ali Khokhar ,&nbsp;Zhang You ,&nbsp;Liu Hui ,&nbsp;Darya Khan ,&nbsp;Qamar U Zaman ,&nbsp;Babar Usman ,&nbsp;Hua-Feng Wang","doi":"10.1016/j.jbiotec.2026.02.001","DOIUrl":"10.1016/j.jbiotec.2026.02.001","url":null,"abstract":"<div><h3>Background</h3><div><em>Selenicereus undatus</em> (<em>S.undatus</em>) is an epiphyte cacti and it is largely grown in Asia. Drought, salinity and heavy metal stress are the major restricting factors in its growth and productivity in nature. Class III peroxidases (PODs) are important genes in response of plants to oxidative and abiotic stresses, as was found by a number of studies. Nevertheless, the genomic structure and functional analyses of POD genes in S. undatus have not been well investigated.</div></div><div><h3>Results</h3><div>In this study, the physiological and molecular responses of <em>Selenicereus undatus</em> to single and multi-factorial environmental stresses were investigated in comparison with melatonin supplementation. However, stress significantly increased the activities of peroxidase (POD), proline content and hydrogen peroxide (H₂O₂) accumulation, which were strongly attenuated by the application of melatonin through maintaining an equilibrium in redox status to promote growth. Transcriptome analysis observed obvious differential expression in the <em>HuPOD</em> gene family when under different stress treatments. In the <em>S. undatus</em> genome 75 <em>HuPOD</em> genes were identified and classified into five phylogenetic subgroups. Among them, <em>HuPOD-02/05/06/12/13/29</em> were strongly up-regulated under combined stress while <em>HuPOD-36/56</em> showed slight downregulation to single stresses conditions. KEGG enrichment analysis revealed that under single and multi-factorial stresses, differentially expressed <em>HuPOD</em> genes were significantly enriched in the MAPK signaling pathway, glutathione metabolism, and phenylpropanoid biosynthesis, suggesting pivotal roles in oxidative stress regulation and signal transduction in S. undatus. RT-qPCR validation confirmed RNA-seq expression patterns for five representative genes (<em>HuPOD-01/10/36/56/62</em>), with <em>HuPOD-10/62</em> showing the highest induction under melatonin-supplemented stresses (Cd+S+D+M).</div></div><div><h3>Conclusion</h3><div>These findings suggested that POD gene family may participate in the response to single and multifactorial stress in <em>S.undatus</em> This study provides a scientific basis for the further development and functional validation of the POD gene family in <em>S.undatus.</em></div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"412 ","pages":"Pages 26-43"},"PeriodicalIF":3.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146165480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamic regulation of ROS stress in the accumulation of L-lysine in Escherichia coli","authors":"Li Zhang,&nbsp;Jianan Yang,&nbsp;Zhixuan Lv,&nbsp;Longjun Han,&nbsp;Zhiwei Zha,&nbsp;Zhijie Cheng,&nbsp;Xu Yang,&nbsp;Zhengyang Xu,&nbsp;Lei Yang,&nbsp;Jian Gao","doi":"10.1016/j.jbiotec.2026.01.011","DOIUrl":"10.1016/j.jbiotec.2026.01.011","url":null,"abstract":"<div><div>Oxidative stress in <em>Escherichia coli</em> during chemical production, caused by reactive oxygen species (ROS), impairs cell viability and limits output. This study addressed product accumulation and ROS stress in <span>L</span>-lysine-producing strains. Genetic engineering created three variants: dynamically up-regulated R1, down-regulated R2, and temporally regulatable R3 to reduce ROS during fermentation. Under stress conditions, all engineered strains showed significantly lower ROS levels versus parental strain R0, with R3 exhibiting the greatest reduction. Fermentation confirmed R3's superior performance, yielding 85.8 % more <span>L</span>-lysine than R0. These results demonstrate that constructing ROS mitigator achieves precise ROS control and efficient <span>L</span>-lysine synthesis. This dynamic regulation strategy enhances cell viability and production performance under oxidative stress, providing a viable approach for improving engineered cell factories' stress resistance.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"411 ","pages":"Pages 66-77"},"PeriodicalIF":3.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146052160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}