Journal of biotechnology最新文献_第2页

Combinatorial metabolic engineering strategy of precursor pools for the yield improvement of spinosad in Saccharopolyspora spinosa 采用前体池组合代谢工程策略提高刺槐多糖的产量。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-11-02 DOI: 10.1016/j.jbiotec.2024.10.010

Li Cao , Xirong Liu , Danlu Yang , Ziyuan Xia , Zirui Dai , Lin Sun , Jing Fang , Zirong Zhu , Duo Jin , Jie Rang , Shengbiao Hu , Liqiu Xia

{"title":"Combinatorial metabolic engineering strategy of precursor pools for the yield improvement of spinosad in Saccharopolyspora spinosa","authors":"Li Cao , Xirong Liu , Danlu Yang , Ziyuan Xia , Zirui Dai , Lin Sun , Jing Fang , Zirong Zhu , Duo Jin , Jie Rang , Shengbiao Hu , Liqiu Xia","doi":"10.1016/j.jbiotec.2024.10.010","DOIUrl":"10.1016/j.jbiotec.2024.10.010","url":null,"abstract":"<div><div>Spinosad is an insecticide produced by <em>Saccharopolyspora spinosa</em>, and its larvicidal activity is considered a promising approach to combat crop pests. The aim of this study was to enhance the synthesis of spinosad through increasing the supply of acyl-CoAs precursor by the following steps. (i) Engineering the β-oxidation pathway by overexpressing key genes within the pathway to promote the synthesis of spinosad. The results showed that the overexpression of <em>fadD</em>, <em>fadE</em>, and <em>fadA</em>1 genes, as well as the co-expression of <em>fadA</em>1 and <em>fadE</em> genes, increased the yield of spinosad by 0.36-fold, 0.89-fold, 0.75-fold and 1.25-fold respectively. (ii) Employing combinatorial engineering of the β-oxidation pathway and ACC/PCC pathway to promote the synthesis of spinosad. The results showed that the co-expression of <em>fadE</em> and <em>pccA,</em> as well as <em>accC</em> and <em>fadE</em>, resulted in a 1.77-fold and 1.43-fold increase in spinosad production respectively. (iii) When exogenous triacylglycerol was added to the fermentation medium, the solely engineering of the β-oxidation pathway increased the yield of spinosad by 7.13-fold, reaching 427.23 mg/L. While the combinatorial engineering of both the β-oxidation pathway and ACC/PCC pathway increased the yield of spinosad by 9.61-fold, reaching 625.17 mg/L, and further optimization of the culture medium resulted in an even higher yield of spinosad, reaching 1293.43 mg/L. The results of this study indicate that the above combination strategy can promote the efficient biosynthesis of spinosad.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 127-139"},"PeriodicalIF":4.1,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An overview of engineering microbial production of nicotinamide mononucleotide 烟酰胺腺嘌呤单核苷酸微生物工程生产概述。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-11-02 DOI: 10.1016/j.jbiotec.2024.10.014

Boting Li, Xiangfeng Meng, Weifeng Liu

引用次数: 0

Analysis of population heterogeneity in CHO cells by genome-wide DNA methylation analysis and by multi-modal single-cell sequencing 通过全基因组 DNA 甲基化分析和多模式单细胞测序分析 CHO 细胞中的群体异质性。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-10-31 DOI: 10.1016/j.jbiotec.2024.10.012

Elias Böhl , Günter Raddatz , Suki Roy , Lingzhi Huang , Jasrene Kaur Sandhu , Emeka Ignatius Igwe , Manuel Rodríguez-Paredes , Florian Böhl , Frank Lyko

{"title":"Analysis of population heterogeneity in CHO cells by genome-wide DNA methylation analysis and by multi-modal single-cell sequencing","authors":"Elias Böhl , Günter Raddatz , Suki Roy , Lingzhi Huang , Jasrene Kaur Sandhu , Emeka Ignatius Igwe , Manuel Rodríguez-Paredes , Florian Böhl , Frank Lyko","doi":"10.1016/j.jbiotec.2024.10.012","DOIUrl":"10.1016/j.jbiotec.2024.10.012","url":null,"abstract":"<div><div>CHO cells are major hosts for the industrial production of therapeutic proteins and their production stability is of considerable economic significance. It is widely known that CHO cells can rapidly acquire genetic alterations, which affects their genetic homogeneity over time. However, the role of non-genetic mechanisms, including epigenetic mechanisms such as DNA methylation, remains poorly understood. We have now used whole-genome bisulfite sequencing to establish single-base methylation maps of eight independent CHO cell lines. Our results identify CpG islands and low-methylated regions as conserved elements with dynamic DNA methylation. Interestingly, methylation patterns were found to cluster clearly along the three main branches of CHO evolution, with no directional changes over short culture periods. Furthermore, multi-ome single-cell sequencing of 9833 nuclei from three independent cultures revealed dynamic subpopulation structures characterized by robust expression differences in pathways related to protein production. Our findings thus provide novel insights into the epigenetic landscape and heterogeneity of CHO cells and support the development of epigenetic biomarkers that trace the emergence of subpopulations in CHO cultures.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 72-79"},"PeriodicalIF":4.1,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor MfbHLH104 from Myrothamnus flabellifolia promotes drought tolerance of Arabidopsis thaliana by enhancing stability of the photosynthesis system 拟南芥转录因子MfbHLH104通过提高光合作用系统的稳定性促进拟南芥的耐旱性。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-10-29 DOI: 10.1016/j.jbiotec.2024.10.011

Zhuo Huang , Xiangying Xiang , Wenxin Xu , Li Song , Rong Tang , Duoer Chen , Qiao Li , Yujue Zhou , Cai-Zhong Jiang

{"title":"The transcription factor MfbHLH104 from Myrothamnus flabellifolia promotes drought tolerance of Arabidopsis thaliana by enhancing stability of the photosynthesis system","authors":"Zhuo Huang , Xiangying Xiang , Wenxin Xu , Li Song , Rong Tang , Duoer Chen , Qiao Li , Yujue Zhou , Cai-Zhong Jiang","doi":"10.1016/j.jbiotec.2024.10.011","DOIUrl":"10.1016/j.jbiotec.2024.10.011","url":null,"abstract":"<div><div>The resurrection plant <em>Myrothamnus flabellifolia</em> can survive extreme drought and desiccation conditions, and quickly recover after rewatering. However, little is known about the mechanism underlying the drought tolerance of <em>M. flabellifolia</em>. In this study, <em>MfbHLH104</em> was cloned and introduced into <em>Arabidopsis thaliana</em> due to the lack of a transgenic system for <em>M. flabellifolia.</em> MfbHLH104 is localized in the nucleus. Its N-terminal region has transactivation ability in yeast, and the C-terminal region may inhibit the transactivation ability. Overexpressing <em>MfbHLH104</em> significantly increased drought and salt tolerance of <em>A. thaliana</em> at both seedling and adult stages<em>.</em> It enhanced leaf water retention capacity by decreasing water loss rate and increasing drought- and abscisic acid (ABA) -induced stomatal closure. Additionally, it boosted osmolyte accumulation and ROS scavenging ability by up-regulating genes associated with osmolyte biosynthesis and antioxidant enzymes, and enhancing antioxidant enzyme activities. The expression of ABA-responsive genes were also promoted by MfbHLH104. Remarkably, RNA-seq analysis indicated that MfbHLH104 significantly up-regulated 32 genes (FDR < 0.05 and fold change ≥1.5) involved in photosynthesis related pathways (KEGG pathway No: ko00195, ko00196) under drought, which account for 18.7 % of the total up-regulated genes and the most enriched KEGG pathways. This result suggested that it may help to maintain the stability of the photosynthesis system under drought conditions.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 89-103"},"PeriodicalIF":4.1,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fractionation of lignin and fermentable sugars from wheat straw using an alkaline hydrogen peroxide/pentanol biphasic pretreatment 使用碱性过氧化氢/戊醇双相预处理法分馏小麦秸秆中的木质素和可发酵糖。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-10-18 DOI: 10.1016/j.jbiotec.2024.10.004

Yue Wu , Hui Zhang , Qixuan Lin , Ruonan Zhu , Lihong Zhao , Xingjie Wang , Junli Ren , Ling Meng

{"title":"Fractionation of lignin and fermentable sugars from wheat straw using an alkaline hydrogen peroxide/pentanol biphasic pretreatment","authors":"Yue Wu , Hui Zhang , Qixuan Lin , Ruonan Zhu , Lihong Zhao , Xingjie Wang , Junli Ren , Ling Meng","doi":"10.1016/j.jbiotec.2024.10.004","DOIUrl":"10.1016/j.jbiotec.2024.10.004","url":null,"abstract":"<div><div>To breakthrough the delignification saturation point (DSP) of alkaline hydrogen peroxide (AHP) pretreatment, a biphasic AHP/pentanol (AHPP) pretreatment was proposed in this work. The temperature and H<sub>2</sub>O<sub>2</sub> concentration were evaluated. Under the optimal conditions (110 °C, 2 h, 4 % H<sub>2</sub>O<sub>2</sub>), 70.73 % of lignin was removed, which was increased by 11.65 % than the traditional AHP pretreatment, indicating successful overcoming of the DSP by adding pentanol. 85.74 % and 88.62 % of glucan and xylan digestibility were achieved, respectively, which increased by 7.41 % and 5.87 % as compared to AHP pretreatment. Furthermore, the lignin extracted from the organic phase accounted for 38.51 % of the delignification, and it had a low molecular weight, effectively preserving the β-O-4 bonds. Finally, satisfied pentanol recovery (77.91 %) and delignification (57.19 %) along with excellent glucan (76.11 %) and xylan (77.52 %) digestibility were reached after fourth recycling of AHPP pretreatment. Therefore, AHPP pretreatment was a promising method for biomass valorization within biorefinery concept.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 62-71"},"PeriodicalIF":4.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid analysis of human primary dermal fibroblasts and epidermal keratinocytes after near-infrared exposure using mass spectrometry imaging 利用质谱成像技术分析近红外照射后人原生真皮成纤维细胞和表皮角质细胞的脂质。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-10-18 DOI: 10.1016/j.jbiotec.2024.10.009

Laura van der Vloet , Zoé Ducarne , Ron M.A. Heeren , Anne C. Berends , Michiel Vandenbosch

{"title":"Lipid analysis of human primary dermal fibroblasts and epidermal keratinocytes after near-infrared exposure using mass spectrometry imaging","authors":"Laura van der Vloet , Zoé Ducarne , Ron M.A. Heeren , Anne C. Berends , Michiel Vandenbosch","doi":"10.1016/j.jbiotec.2024.10.009","DOIUrl":"10.1016/j.jbiotec.2024.10.009","url":null,"abstract":"<div><div>Photobiomodulation (PBM) therapy is the application of near-infrared (NIR) exposure to injuries or lesions to (among others) improve wound healing, reduce inflammation, and decreases acute and chronic pain. However, the understanding of the molecular mechanism of PBM, more specifically the effects of NIR on skin cells is still lacking behind. Lipids are essential components of cellular membranes that are integral to skin structure and function. This study aims to elucidate the impact of NIR exposure on the skin’s lipidome by investigating the molecular effect of NIR exposure on single skin cells. Primary human dermal fibroblasts (NHDFa) and human epidermal keratinocytes (HEKa) were exposed to NIR (850 nm) with a dose of 6.5 J/cm<sup>2</sup> for 5 consecutive days between 09.00 and 12.00 am. A workflow utilizing matrix-assisted laser desorption/ionization mass spectrometry imaging combined with liquid chromatography tandem mass spectrometry for lipidomics analysis was performed. This study provides evidence that adequate exposure of NIR influences lipid metabolism in NHDFa, whereas no alterations were found in HEKa. This work lays the groundwork in explaining the beneficial properties on both skin-related effects and systemic health benefits as seen in clinical studies.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 53-61"},"PeriodicalIF":4.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In-line monitoring of bioreactor by Raman spectroscopy: Direct use of a standard-based model through cell-scattering correction 利用拉曼光谱对生物反应器进行在线监测：通过细胞散射校正直接使用基于标准的模型。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-10-18 DOI: 10.1016/j.jbiotec.2024.10.007

Ning Yang , Cédric Guerin , Ninel Kokanyan , Patrick Perré

{"title":"In-line monitoring of bioreactor by Raman spectroscopy: Direct use of a standard-based model through cell-scattering correction","authors":"Ning Yang , Cédric Guerin , Ninel Kokanyan , Patrick Perré","doi":"10.1016/j.jbiotec.2024.10.007","DOIUrl":"10.1016/j.jbiotec.2024.10.007","url":null,"abstract":"<div><div>Raman spectroscopy and machine learning have become popular in in-line monitoring of bioreactors. However, traditional modeling processes typically entail extensive fermentation batches to collect learning datasets, which are significantly time–consuming and laborious. In addition, these models are limited to configurations with the same conditions as the training batches. The present work proposes a reproducible and adaptable modeling approach by combining standard spectra as a training dataset, with a simple means of correcting for cell scattering. Alcoholic fermentation by <em>Saccharomyces cerevisiae</em> is used as a benchmark. Initially, a partial least squares (PLS) regression model was developed based on the spectra of pure solutions of glucose and ethanol. Then, a mathematical expression was defined to estimate yeast concentration, allowing the correction of Raman intensity attenuated by cell scattering. The corrected spectra demonstrate close alignment with reference spectra in both shape and intensity. Validation of the methodology was conducted across numerous batches and one fed–batch bioreactor. As a result, the developed method enables the simultaneous monitoring of glucose, ethanol, and yeast concentrations, effectively addressing the challenge of implementing an independent standards based PLS model to manage the intricate compositional dynamics in bio–processes. The conclusion underscores the effectiveness of the proposed method and offers new prospects in biotechnological industries.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 41-52"},"PeriodicalIF":4.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation into optimizing fermentation processes to enhance uric acid degradation by probiotics 关于优化发酵过程以提高益生菌降解尿酸能力的研究。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-10-18 DOI: 10.1016/j.jbiotec.2024.10.008

Hanwei Zheng , Zhiqing Liu , Hao Zheng , Yuman Miao , Chenyu Liu , Minhua Zong , Wenyong Lou

引用次数: 0

Metabolic engineering of Halomonas bluephagenesis for the production of ethylene glycol and glycolate from xylose 从木糖生产乙二醇和乙醇酸盐的蓝光单胞菌代谢工程。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-10-15 DOI: 10.1016/j.jbiotec.2024.10.006

Yuzhong Liu , Kai Huo , Biwei Tan , Xulin He , Qiong Wu , Zheng-Jun Li

引用次数: 0

Recycling potential of Cupriavidus necator for life support in space: Production of SCPs from volatile fatty acid and urea mixture 用于太空生命支持的Cupriavidus necator的回收潜力：利用挥发性脂肪酸和尿素混合物生产 SCPs

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-10-12 DOI: 10.1016/j.jbiotec.2024.10.001

P. Joris , E. Lombard , A. Paillet , G. Navarro , S.E. Guillouet , N. Gorret

{"title":"Recycling potential of Cupriavidus necator for life support in space: Production of SCPs from volatile fatty acid and urea mixture","authors":"P. Joris , E. Lombard , A. Paillet , G. Navarro , S.E. Guillouet , N. Gorret","doi":"10.1016/j.jbiotec.2024.10.001","DOIUrl":"10.1016/j.jbiotec.2024.10.001","url":null,"abstract":"<div><div>The International Space Station currently requires four annual replenishments for food supply, a practice that won't be feasible for deep space missions due to the greater distances. Based on the design of closed ecological life support systems, two waste streams were identified: urea from the crew urine, volatile fatty acids (VFAs) from a first stage of anaerobic digestion of waste. The objective of this study was to assess the ability of bacterium <em>Cupriavidus necator</em> to produce single cell protein on urea and VFAs. Thus, the effect of carbon sources (glucose <em>vs</em> VFAs) and the dilution rate on the biomass composition was determined in continuous cultures. Complete transformation of the carbon source into protein-rich biomass was achieved up to 78 % cell dry weight (CDW). For both carbon sources, the protein content increased from 55.0 %CDW to 78 %CDW with a decrease in the dilution rate. Conversely, the nucleic acid and polyhydroxyalkanoate contents decreased with the dilution rate from 8.8 %CDW to 4.8 %CDW and 9.8 %CDW to 0.6 %CDW respectively. Working at a low dilution rate seems to be a good way to maximize protein content while minimizing unwanted nucleic acids and polyhydroxyalkanoates.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 18-27"},"PeriodicalIF":4.1,"publicationDate":"2024-10-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142446891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0