Journal of biotechnology最新文献

{"title":"Integrative omics approaches for bioactive metabolite discovery in marine macroalgae: Recent advances and future perspectives","authors":"M. Marimuthu ,&nbsp;M. Anitha ,&nbsp;P. Prakash ,&nbsp;Meivelu Moovendhan","doi":"10.1016/j.jbiotec.2026.01.007","DOIUrl":"10.1016/j.jbiotec.2026.01.007","url":null,"abstract":"<div><div>Phlorotannins, bromophenols, sulfated polysaccharides, terpenoids, lipids and halogenated molecules exhibit potent antioxidant, anticancer, anti-inflammatory, and antimicrobial properties. Conventional discovery methods, such as solvent extraction and bioassay-guided fractionation, are limited by low throughput, specificity, and poor insight into biosynthetic mechanisms. High-throughput omics platforms including genomics, transcriptomics, proteomics, and metabolomics have transformed the discovery of bioactive metabolites in macroalgae by unraveling system-wide interpretation of biosynthetic pathways. This review outlines omics-driven strategies for macroalgal natural product research, highlighting genome sequencing and annotation efforts. Transcriptome-based biosynthetic gene cluster (BGC) mining, and gene regulation analysis via RNA-seq. Proteomic approaches such as 2D electrophoresis, LC-MS/MS, and MALDI-TOF are discussed for their role in elucidating enzyme functions and post-translational modifications. Metabolomics tools, including GC-MS, LC-MS, and NMR, paired with platforms like GNPS and MetaboAnalyst, have improved metabolite identification, dereplication and pathway analysis. Emerging integrative multi-omics frameworks combining machine learning and systems biology are paving the way for predictive, precision-driven bioprospecting. Nonetheless, challenges persist, including incomplete genome assemblies, taxonomic inconsistencies and limited reference databases. This review emphasizes how omics technologies are deciphering and mechanistically elucidating the untapped metabolic potential of marine macroalgae, accelerating their application in biotechnology and therapeutic innovation.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"411 ","pages":"Pages 13-28"},"PeriodicalIF":3.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two industrial media reveal a mitochondrial disfunction in CHO cell cultures co-fed with glucose and lactic acid","authors":"Keegan Orzechowski ,&nbsp;Johanna Vappiani-Korben ,&nbsp;Daniel C. Sevin ,&nbsp;Juan Aon","doi":"10.1016/j.jbiotec.2026.01.002","DOIUrl":"10.1016/j.jbiotec.2026.01.002","url":null,"abstract":"<div><div>Metabolomics analyses of cell culture processes can provide valuable insight into cellular physiology that can be leveraged to develop more productive processes. In this work, we applied metabolomics to interrogate CHO cell behavior in two industrial chemically-defined media in cultures co-fed with glucose and lactic acid. We previously reported that secreted acylcarnitines are indicative of altered mitochondrial metabolism when cultures are fed lactic acid and serve to maintain homeostasis between free CoA, acetyl-CoA, free carnitines, and acylcarnitines (Vappiani <em>et al</em>., 2021). One of the two media (“Medium B”) increased significantly viable-cell count and antibody titer than Medium A. Here, we report that CHO’s mitochondrial dysfunctionality based on the secretion of acylcarnitines in lactic acid-fed cultures depends on the overall medium composition. We hypothesize that in order to achieve better growth and titer, Medium B exhibited an increased oxidative phosphorylation based on the lower secretion of acylcarnitines and a differential utilization of riboflavin and thiamine, precursors of coenzymes required to enhance mitochondrial pyruvate incorporation and TCA cycle function. Therefore, our data provides further evidence that non-obvious changes to medium composition can have substantial effects on CHO-based production processes by altering the activity of oxidative phosphorylation required for the proper functioning of mitochondria but also for better antibody production.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"411 ","pages":"Pages 1-11"},"PeriodicalIF":3.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145933438","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"De novo biosynthesis of lavandulol and lavandulyl acetate in Escherichia coli","authors":"Kefa Hu ,&nbsp;Zhipeng Qi ,&nbsp;Xiaode Huang ,&nbsp;Lei Wang ,&nbsp;Xiaomeng Zhang ,&nbsp;Shaoheng Tang","doi":"10.1016/j.jbiotec.2026.01.014","DOIUrl":"10.1016/j.jbiotec.2026.01.014","url":null,"abstract":"<div><div>Lavandulol and its acetate ester, lavandulyl acetate, are valuable irregular monoterpenes found in lavender essential oil, widely utilized in the food and cosmetic industries. Due to the limitations of extraction from natural plant resources, developing sustainable microbial cell factories for their production is of great interest. Here, we report the first de novo biosynthesis of lavandulol and lavandulyl acetate in <em>Escherichia coli</em>. Initially, a heterologous mevalonate (MVA) pathway was coupled with lavandulyl diphosphate synthase (LPPS) to establish the biosynthetic route from glycerol. To relieve pathway bottlenecks, we overexpressed the endogenous dITP/XTP pyrophosphatase RdgB, which efficiently facilitated the dephosphorylation of the precursor lavandulyl diphosphate (LPP), increasing lavandulol titers to 42.87 mg/L. Subsequently, the alcohol acyltransferase ATF1 from <em>Saccharomyces cerevisiae</em> was identified as the most effective enzyme for acetylating lavandulol. By integrating ATF1 into the pathway and optimizing both the host strain and fermentation process, we achieved a final lavandulyl acetate titer of 89.43 mg/L. This study establishes a promising prokaryotic platform for the efficient biosynthesis of high-value irregular monoterpenes.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"411 ","pages":"Pages 144-151"},"PeriodicalIF":3.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streptomyces bottropensis SY8-derived eumelanin exhibits skin wound healing activity and prevents H2O2 or UV-induced skin aging","authors":"Sevval Yildirim ,&nbsp;Buket Bakan ,&nbsp;Meryem Doymus ,&nbsp;Seydanur Elmas ,&nbsp;Nazli Pinar Arslan ,&nbsp;Mesut Taskin","doi":"10.1016/j.jbiotec.2026.01.009","DOIUrl":"10.1016/j.jbiotec.2026.01.009","url":null,"abstract":"<div><div><em>Streptomyces</em>-derived melanins exhibit diverse bioactive properties. This study aims to (1) produce melanin production from <em>Streptomyces bottropensis</em> SY8 (GenBank accesion number: PQ565816), (2) characterize the melanin, and (3) investigate its bioactive properties (toxicity and irritancy as well as <em>invitro</em> wound healing and anti-skin aging activities). Under optimized culture conditions, melanin production of 3.26 g/L was achieved in shaking flask cultures of the bacterium. The purified melanin was characterized as eumelanin. Compared to the control (Vitamin C), the purified melanin was found to have moderate antioxidant activity in radical scavenging assays. It did not cause toxicity on fibroblast cell line and irritancy in HET-CAM test (hen's egg test on chorioallantoic membrane test). The melanin reduced reactive oxygen species (ROS) accumulation and cell senescence induced by H₂O₂ or UV in fibroblast cells, indicating its anti-skin aging potential. When compared the control (melanin free), the melanin doses of 500 µg/mL and 1000 µg/mL caused increments of 17.59 % and 24.53 % in wound closure ratios at the end of the 24th hour, respectively. This is the first report on melanin production from <em>S. bottropensis</em>. Besides, <em>in vitro</em> wound healing and anti-skin aging activities of <em>Streptomyces</em>-derived melanins were investigated for the first time. Furthermore, HET-CAM test was used for the first time to analyze irritancy property of melanins. The results of this study indicate that <em>S. bottropensis</em> SY8-derived eumelanin can be exploited as an ingredient of anti-aging creams or wound dressings.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"411 ","pages":"Pages 40-53"},"PeriodicalIF":3.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146036093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Couple action of some proteases from feather keratin-degrading bacterium B.licheniformis CP-16","authors":"Shijin Luo ,&nbsp;Deshan Wang ,&nbsp;Kaige Yang ,&nbsp;Hui Ye ,&nbsp;Yingxia Sun ,&nbsp;Zhiguo Qi ,&nbsp;Jianjun Zuo ,&nbsp;Tieying Zhang","doi":"10.1016/j.jbiotec.2026.01.006","DOIUrl":"10.1016/j.jbiotec.2026.01.006","url":null,"abstract":"<div><div>A keratin-degrading bacterium, <em>Bacillus licheniformis</em> CP-16 (<em>B.licheniformis</em> CP-16), isolated from feather waste, was found to produce three extracellular proteins with protease activity. Following mass spectrometry sequencing and BLAST analysis, these proteins exhibited high similarity to γ-glutamyl transpeptidase, alkaline serine protease and thioredoxin-like protein YkuU, respectively. Genes of the three proteins and Ker A from <em>B.licheniformis</em> were successfully cloned into pET22b and expressed in <em>Escherichia coli</em> (<em>E coli</em>), and the four recombinant enzymes were named P-Glu, P-Alk, P-Trx, and P-Ker. P-ker showed great keratinase activity (4.9 kU/mg) and casein protease activity (6.5 kU/mg), as P-Alk showed keratinase activity (1.2 kU/mg) and casein protease activity (23 kU/mg). In contrast, both P-Glu and P-Trx displayed low levels of keratinase and casein protease activities. P-Trx had disulfide bond reductase activity (3 U/mg). When mixing with P-Trx, the other three proteases, P-Glu, P-Alk, P-Ker degraded feather keratin with keratinase activity promotion of 71 %, 40 % and 71 %. Commercial proteases (trypsin and chymotrypsin) also got keratinolytic capacity after mixing with P-Trx. These findings reveal a novel synergistic mechanism between P-Trx and other proteases in the degradation of feather keratin, leading to more efficient keratin breakdown.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"411 ","pages":"Pages 130-143"},"PeriodicalIF":3.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145989140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficiency and scalability in harvesting mammalian cell cultures: A scale-down approach to continuous centrifugation","authors":"Michaela Dölle ,&nbsp;Maxime Hervé ,&nbsp;Staffan Königsson ,&nbsp;Peter Thorwid ,&nbsp;Johan Rockberg ,&nbsp;Véronique Chotteau","doi":"10.1016/j.jbiotec.2026.01.012","DOIUrl":"10.1016/j.jbiotec.2026.01.012","url":null,"abstract":"<div><div>Continuous centrifugation is a well-established method for clarifying mammalian cell cultures, but traditional batch-based approaches often fall short of modern biomanufacturing’s scalability and flexibility demands. Increasing variability in product volumes and manufacturing setups calls for adaptable, scalable solutions. To address this, we developed a scaled-down continuous centrifuge (“Mini”) based on a commercial disc-stack centrifuge, facilitating efficient early-stage development and improving technology transfer and scale-up. This study demonstrates the Mini’s potential to bridge the gap between small-scale optimization and industrial-scale centrifugation. Proof-of-concept experiments with Chinese hamster ovary cell culture confirmed its separation efficiency, achieving low turbidity, high product recovery (up to 98.5%), and minimal cell stress. Lactate dehydrogenase activity remained low, with a maximum increase in host cell proteins of 11.9% across various operating conditions. Validation experiments against the pilot-scale Culture One™ Primo showed comparable or superior turbidity reduction and lower lactate dehydrogenase activity, highlighting the Mini’s gentle cell handling. The Mini enables continuous small-scale centrifugation while replicating key performance parameters of the pilot-scale system, ensuring accurate performance predictions and reliable scale-up. It provides a scalable, flexible solution that meets the evolving needs of modern biomanufacturing for efficient and adaptable clarification processes.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"411 ","pages":"Pages 89-101"},"PeriodicalIF":3.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Marine-derived Streptomyces sp. isolated from the Persian Gulf as a novel source of melanin","authors":"Seyed Muhammad Hamid Malekpour ,&nbsp;Moj Khaleghi ,&nbsp;Alireza Akhtarpoor ,&nbsp;Mostafa Pournamdari ,&nbsp;Hoda KeshmiriNeghab ,&nbsp;Farideh MohammadHosseinZadeh","doi":"10.1016/j.jbiotec.2026.01.005","DOIUrl":"10.1016/j.jbiotec.2026.01.005","url":null,"abstract":"<div><div>In this study, the structure and biological properties of melanin pigment produced by Streptomyces strain SEA5 isolated from Persian Gulf sediments were investigated. This strain was cultured in an enriched culture medium (<span>L</span>-tyrosine, meat extract). Pigment production was also performed in culture media containing different sources of amino acid <span>L</span>-tyrosine such as soybean meal. Pigment extraction was performed using hydrochloric acid (6 M). In order to investigate its physicochemical nature, TLC, HPLC, UV-Vis spectroscopy, FTIR and 1 H NMR analysis was performed. On the other hand, strong UV absorption with SPF, antioxidant activity and cytotoxicity assay of this pigment were also studied. The results showed that strain SEA5 was well grown and melanized well (1.5 g/L) in medium containing <span>L</span>-tyrosine. It was 1.36 g/L when cultured in medium containing soybean meal. The UV–vis spectrum had an absorption peak at 225 nm. FTIR identified the functional groups of eumelanin with very high peaks at 3414.46 cm-1 (N-H group) and 1650–1540 cm-1 (C<img>O and C<img>N vibrations). Also, the ¹HNMR spectrum showed several peaks between 6.9 and 8.0 ppm, indicating aromatic protons. On the other hand, the extracted pigment had an SPF of 22.55 and showed significant antioxidant activity with 60–77 % reduction of DPPH radicals. The pigment in the formulation selectively inhibited the growth of A375 melanoma cells (IC50- 79.82 μg/mL), whilst it was non-toxic when tested against normal human dermal fibroblasts and HaCaT keratinocytes. Overall, these findings suggest the potential of SEA5-derived melanin as a human skin care product, pharmaceutical product and antioxidant material.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"411 ","pages":"Pages 78-88"},"PeriodicalIF":3.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145989190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum to “Structure–function analysis and exonuclease deletion yield an improved strand-displacing DNA polymerase from Aeribacillus pallidus for efficient recombinase polymerase amplification” [J. Biotechnol. 410 (2026) 321–330]","authors":"Koki Nishi ,&nbsp;Eisuke Inoue ,&nbsp;Yuto Murakami ,&nbsp;Yuri Ishii ,&nbsp;Yukiko Nakura ,&nbsp;Itaru Yanagihara ,&nbsp;Kiyoshi Yasukawa ,&nbsp;Shinsuke Fujiwara","doi":"10.1016/j.jbiotec.2026.01.003","DOIUrl":"10.1016/j.jbiotec.2026.01.003","url":null,"abstract":"","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"411 ","pages":"Page 12"},"PeriodicalIF":3.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145981351","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative experimental and computational approach for the development of novel plasminogen activators from truncated streptokinase fragments","authors":"Vijay Gunasekaran ,&nbsp;Suthakaran Pichaimuthu ,&nbsp;Vigneshwaran Namasivayam ,&nbsp;V. Ponnusami","doi":"10.1016/j.jbiotec.2026.01.004","DOIUrl":"10.1016/j.jbiotec.2026.01.004","url":null,"abstract":"<div><div>Streptokinase (SK), a widely used thrombolytic agent, activates human plasminogen, which in turn dissolves blood clots. However, its clinical application is limited because it elicits immunogenic responses in patients. The present study aims to develop novel plasminogen activators derived from truncated fragments of SK that retain activity comparable to the full-length SK enzyme. Based on the structure-function relationships and the previous literature, ten fragments of SK were designed by systematically truncating the N- and C-terminal regions and the central β-domain. These fragments included variants lacking N-terminal residues (amino acids 1–24 or 1–37), C-terminal truncations (beyond amino acid 300) and segments focused on the central domain (starting at amino acid 130 or ending at 173). Fragments were expressed in <em>E. coli</em> and their plasminogen activation activity was evaluated. The experimental results indicated that fragments containing an intact N1 domain retained a high level of plasminogen activation activity (fragments 1–300, 1–173), while deletion of key N-terminal residues significantly reduced it. C-terminal fragments partially compensated for N-terminal loss (fragment 130–414), but overall activity remained lower. Molecular modelling studies of protein–protein complex interface analyses revealed that the N1 domain (residues 1–173) of SK constitutes the principal binding interface with HPlg, with specific N-terminal residues serving as interaction hotspots. Residue-level contact mapping correlated strongly with experimental activity, underscoring the importance of precise interface residues rather than overall domain-level contact coverage. The study identifies SK fragments 1–300, 1–173 and 130–414 as having substantial activity comparable to full-length SK, suggesting that these constructs could serve as the basis for novel thrombolytic drug candidates pending further immunogenicity evaluation. These interesting findings provide both structural and functional insights for the rational design of improved streptokinase-derived therapeutics.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"411 ","pages":"Pages 29-39"},"PeriodicalIF":3.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145997740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antibacterial nut grass cellulose reinforced polylactic acid nanocomposites: A holistic assessment for biomedical scaffolds","authors":"Priya S.A.,&nbsp;Nevaditha N.T.","doi":"10.1016/j.jbiotec.2026.01.008","DOIUrl":"10.1016/j.jbiotec.2026.01.008","url":null,"abstract":"<div><div>Poly(lactic acid) (PLA) composites reinforced with various natural fibers have been extensively investigated for biomedical applications. However, the use of nut grass-derived cellulose nanofibers (CNFs) remains unexplored in the current literature. This study presents the development of PLA nanocomposites reinforced with CNFs extracted from nut grass for biomedical applications. The PLA matrix was chemically modified using ethylene glycol dimethacrylate (EGDMA) and plasticized with castor oil. SEM analysis revealed uniform dispersion of CNFs at lower concentrations, leading to improved film morphology. XRD analysis indicated a CNF particle size of approximately 26 nm. Mechanical testing showed significant enhancements in material strength, with tensile strength and Young’s modulus increasing by 97.1 % and 113.9 %, respectively. The nanocomposites showed greater degradation in alkaline than acidic medium due to accelerated hydrolysis. Antibacterial activity against <em>Staphylococcus aureus</em> and <em>Pseudomonas aeruginosa</em> increased with CNF content. Hemolysis assays revealed reduced red blood cell damage compared to plain PLA, indicating favorable hemocompatibility. Additionally, MTT assay confirmed low cytotoxicity of the nanocomposites toward PBMC cells. These results demonstrate the potential of PLA nanocomposites as biocompatible, multifunctional materials suitable for applications in implants, wound healing and regenerative medicine.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"411 ","pages":"Pages 54-65"},"PeriodicalIF":3.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146040224","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}