Journal of biotechnology最新文献_第2页

Synthesis of melanin-like amino acid surfactant with enzymatic hydrolysates from silk degumming water 用蚕丝脱胶水中的酶水解物合成黑色素样氨基酸表面活性剂。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-08-22 DOI: 10.1016/j.jbiotec.2024.08.011

{"title":"Synthesis of melanin-like amino acid surfactant with enzymatic hydrolysates from silk degumming water","authors":"","doi":"10.1016/j.jbiotec.2024.08.011","DOIUrl":"10.1016/j.jbiotec.2024.08.011","url":null,"abstract":"<div><p>The degummed wastewater from silk processing contains a huge amount of amino acids and polypeptides from sericin. The silk degumming water is far from being exploited fully. Sericin in the degumming water is generally wasted and causes environmental pollution. In this study, simulated silk degumming water was hydrolyzed by alkaline protease to produce abundant amino acids and polypeptides. After enzymatic hydrolysis, the maximum free amino groups concentration in the silk degumming water was approximately 54 mM. It facilitated the recycling of silk degumming water for the production of melanin-like amino acid surfactants as raw materials. 4-Tert-butylcatechol was used as the starting material to generate o-quinone via oxidation by ceric ammonium nitrate. o-Quinone was coupled with free amino groups in enzymatic hydrolysates of silk degumming water to synthesize a sericin-based amino acid surfactant as hydrophobic and hydrophilic group, respectively. Through the green and simple synthesis route, the product was characterized to have a novel melanin-like structure. The product exhibited superior surface-active properties by lowering the surface tension to 32.39 mN m<sup>−1</sup>. Furthermore, it demonstrated good foaming ability and foam stability, with the initial foam volume of 37 mL and the foam half-life time of more than 25 min. The product owned a good emulsification ability in the oil-water emulsion with delamination time of 297 s and 291 s for emulsion formed by soybean oil and liquid paraffin, respectively. The wetting time of the canvas sheet was only 134 s. Consequently, the product showed low surface tension, good foaming, emulsifying, and wetting properties.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142046749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced molecular stability of ApxII antigen during secretion in Corynebacterium glutamicum by rational design 通过合理设计增强谷氨酸棒杆菌分泌过程中 ApxⅡ 抗原的分子稳定性。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-08-20 DOI: 10.1016/j.jbiotec.2024.08.003

{"title":"Enhanced molecular stability of ApxII antigen during secretion in Corynebacterium glutamicum by rational design","authors":"","doi":"10.1016/j.jbiotec.2024.08.003","DOIUrl":"10.1016/j.jbiotec.2024.08.003","url":null,"abstract":"<div><p>ApxII is a vaccine antigen used to protect against porcine contagious pleuropneumonia, which is a significant threat to the pig industry. Here, we aimed to improve the proteolytic degradation stability of ApxII during its secretion by establishing a complete screening process of stable variants through bioinformatics and site-directed mutagenesis. We employed a combination of semi-rational and rational design strategies to create 34 single-point variants of ApxII. Among them, R114E and T115D variants exhibited better stability without compromising antigen activity. Furthermore, we constructed a multi-site variant, R114E/T115D, which demonstrated the best stability, activity, and yield. Protein stability and molecular dynamic analysis indicated that the greater solubility and lower structural expansion coefficient might explain the increased stability of R114E/T115D. Additionally, site T115 was identified as a key point of truncated ApxII stability. The R114E/T115D variant, with its proven stability and intact antigenic activity, holds promising prospects for industrial-scale applications in the prevention of porcine contagious pleuropneumonia.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142035871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant-based synthesis, characterization approaches, applications and toxicity of silver nanoparticles: A comprehensive review 银纳米粒子的植物合成、表征方法、应用和毒性：全面回顾。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-08-17 DOI: 10.1016/j.jbiotec.2024.08.009

{"title":"Plant-based synthesis, characterization approaches, applications and toxicity of silver nanoparticles: A comprehensive review","authors":"","doi":"10.1016/j.jbiotec.2024.08.009","DOIUrl":"10.1016/j.jbiotec.2024.08.009","url":null,"abstract":"<div><p>The development of an environmentally benign method for the synthesis of nanoparticles has been facilitated by green chemistry. \"Green synthesis\" uses a range of biological elements like microbes, plants, and other biodegradable materials to produce NPs. Active biomolecules that are secreted by natural strains and present in the plant extracts serve as both reducing and capping/stabilizing agents. Microorganisms' intracellular enzymes can reduce metal ions, which explains how NPs might potentially nucleate. Plant-based synthesis of nanomaterials is particularly promising owing to abundant resources, simplicity of synthesis, and low cost. Silver nanoparticles (AgNPs) are attracting great attention in the research community due to their wide variety of applications in chemistry, food technology, microbiology, and biomedicine. Recent years have seen a large amount of research on the bio-genic synthesis of AgNPs employing biomaterials like plant extract and bacteria as reducing agents. Herein we discuss a thorough overview of the plant-based synthesis of silver nanoparticles (AgNPs), characterization approaches, applications, and toxicity. The review covers the green chemistry and nanotechnology elements of producing AgNPs, including a thorough discussion of the plant extract mediated synthesis, detailed formation mechanism, and a well-balanced emphasis on hazards and advantages. Based on current developments, the optimisation strategies, applications, and interdisciplinary characteristics are also covered in detail.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142004266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nanozymes targeting mitochondrial repair in disease treatment 以线粒体修复为目标的纳米酶在疾病治疗中的应用。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-08-17 DOI: 10.1016/j.jbiotec.2024.08.008

{"title":"Nanozymes targeting mitochondrial repair in disease treatment","authors":"","doi":"10.1016/j.jbiotec.2024.08.008","DOIUrl":"10.1016/j.jbiotec.2024.08.008","url":null,"abstract":"<div><p>Mitochondria are crucial sites for biological oxidation and substance metabolism and plays a vital role in maintaining intracellular homeostasis. When mitochondria undergo oxidative damage or dysfunction, they can harm the organism, leading to various reactive oxygen species (ROS)-related diseases. Therefore, therapies targeting mitochondria are a strategy for treating multiple diseases. Many nanozymes can mimic antioxidant enzymes, which enables them to eliminate ROS to mitigate mitochondrial dysfunction. The therapeutic approaches and drugs targeting the mitochondrial electron transport chain (ETC) have emerged as effective treatments for oxidative stress-related diseases resulting from mitochondrial respiratory chain disorders. Therefore, nanozymes that can regulate homeostasis in the mitochondrial ETC have emerged as effective therapeutic agents for treating oxidative stress-related diseases. In addition, benefit from the controllability and modifiability of nanozymes, their modification with TPP, SS-31 peptide, and mitochondrial permeability membrane peptide to eliminate ROS and repair mitochondrial function. The nanozymes that specifically target mitochondria are powerful tools for the treatment of ROS-associated disorders. We discussed the design strategies pertaining to mitochondrion-targeted nanozymes to treat various diseases to develop more efficacious nanozyme tools for the treatment of ROS-related diseases in the future.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142004265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular production of azurin by reusable magnetic Fe3O4 nanoparticle-immobilized Pseudomonas aeruginosa 可重复使用的磁性 Fe3O4 纳米粒子固定铜绿假单胞菌胞外生产氮杂环苷。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-08-17 DOI: 10.1016/j.jbiotec.2024.08.010

{"title":"Extracellular production of azurin by reusable magnetic Fe3O4 nanoparticle-immobilized Pseudomonas aeruginosa","authors":"","doi":"10.1016/j.jbiotec.2024.08.010","DOIUrl":"10.1016/j.jbiotec.2024.08.010","url":null,"abstract":"<div><p>Azurin, found in the periplasm of <em>Pseudomonas aeruginosa</em>, has garnered significant attention as a potential anticancer agent in recent years. High-level secretion of proteins into the culture medium, offers a significant advantage over periplasmic or cytoplasmic expression. In this study, for the first time, <em>P. aeruginosa</em> cells were immobilized with magnetic nanoparticles (MNPs) to ensure effective, simple and quick separation of the cells and secretion of periplasmic azurin protein to the culture medium. For this purpose, polyethyleneimine-coated iron oxide (Fe<sub>3</sub>O<sub>4</sub>@PEI) MNPs were synthesized and MNPs containing Fe up to 600 ppm were found to be non-toxic to the bacteria. The highest extracellular azurin level was observed in LB medium compared to peptone water. The cells immobilized with 400 ppm Fe-containing MNPs secreted the highest protein. Lastly, the immobilized cells were found suitable for azurin secretion until the sixth use. Thus, the magnetic nanoparticle immobilization method facilitated the release of azurin as well as the simple and rapid separation of cells. This approach, by facilitating protein purification and enabling the reuse of immobilized cells, offers a cost-effective means of protein production, reducing waste cell formation, and thus presents an advantageous method.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142004264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a new extraction method and functional analysis of phycocyanobilin from unique filamentous cyanobacteria. 从独特的丝状蓝藻中提取藻蓝蛋白的新方法和功能分析的开发。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-08-15 DOI: 10.1016/j.jbiotec.2024.08.006

Jinichi Aoki, Takashi Yarita, Morifumi Hasegawa, Munehiko Asayama

{"title":"Development of a new extraction method and functional analysis of phycocyanobilin from unique filamentous cyanobacteria.","authors":"Jinichi Aoki, Takashi Yarita, Morifumi Hasegawa, Munehiko Asayama","doi":"10.1016/j.jbiotec.2024.08.006","DOIUrl":"https://doi.org/10.1016/j.jbiotec.2024.08.006","url":null,"abstract":"<p><p>As current methods of production of phycocyanobilin, a photosynthetic blue pigment derived from phycocyanin of filamentous cyanobacteria, Pseudanabaena sp. ABRG5-3, Limnothrix sp. SK1-2-1, and Spirulina sp., exhibit a low extraction efficiency, a new extraction method using ethanol extraction as a type of solvolysis with an autoclave (130 ℃, 5.7bar, 10min) was developed in this study. This method exhibited high efficiency and enabled easy recovery of the three types of phycocyanobilins. The identity of the three types of phycocyanobilins was confirmed by high-performance liquid chromatography and electrospray ionization-tandem mass spectrometry. Phycocyanobilins were stable at high temperatures (80 ℃) and acidic (pH 3) conditions. Phycocyanobilins also possessed a remarkable antioxidant property. This is the first time that a simple phycocyanobilin extraction method with a recovery rate of more than 60% and approximately 1% per dry cell weight of filamentous cyanobacteria has been demonstrated. This novel production method is thus convenient and effective for obtaining high-purity phycocyanobilins.</p>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polydopamine-functionalized polyethersulfone membrane: A paradigm advancement in the field of α-amylase stability and immobilization 聚多巴胺功能化聚醚砜膜：α-淀粉酶稳定性和固定化领域的典范进展。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-08-15 DOI: 10.1016/j.jbiotec.2024.08.007

{"title":"Polydopamine-functionalized polyethersulfone membrane: A paradigm advancement in the field of α-amylase stability and immobilization","authors":"","doi":"10.1016/j.jbiotec.2024.08.007","DOIUrl":"10.1016/j.jbiotec.2024.08.007","url":null,"abstract":"<div><p>Biocatalytic membranes have great potential in various industrial sectors, with the immobilization of enzymes being a crucial stage. Immobilizing enzymes through covalent bonds is a complex and time-consuming process for large-scale applications. Polydopamine (PDA) offers a more sustainable and eco-friendly alternative for enzyme immobilization. Therefore, surface modification with polydopamine as mussel-inspired antifouling coatings has increased resistance to fouling. In this study, α-amylase enzyme was covalently bound to a bioactive PDA-coated polyethersulfone (PES) membrane surface using cyanuric chloride as a linker. The optimal activity of α-amylase enzyme immobilized on PES/PDA membrane was obtained at temperature and pH of 55°C and 6.5, respectively. The immobilized enzyme can be reused up to five reaction cycles with 55 % retention of initial activity. Besides, it maintained 60 % of its activity after being stored for five weeks at 4°C. Additionally, the immobilized enzyme demonstrated increased Michaelis constant and maximum velocity values during starch hydrolysis. The results of the biofouling experiment of various membranes in a dead-end cell demonstrated that the PES membrane’s water flux increased from 6722.7 Lmh to 7560.2 Lmh after PDA modification. Although α-amylase immobilization reduced the flux to 7458.5 Lmh due to enhanced hydrophilicity, compared to unmodified membrane. The findings of this study demonstrated that the membrane produced through co-deposition exhibited superior hydrophilicity, enhanced coating stability, and strong antifouling properties, positioning it as a promising candidate for industrial applications.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of Stephania tetrandra S. MOORE hairy root culture process for tetrandrine production 开发用于生产四氢化噻吩的 Stephania tetrandra S. MOORE 发根培养工艺。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-08-14 DOI: 10.1016/j.jbiotec.2024.08.005

{"title":"Development of Stephania tetrandra S. MOORE hairy root culture process for tetrandrine production","authors":"","doi":"10.1016/j.jbiotec.2024.08.005","DOIUrl":"10.1016/j.jbiotec.2024.08.005","url":null,"abstract":"<div><p>Tetrandrine, a bioactive active compound mainly found in the roots of <em>Stephania tetrandra,</em> exhibits various pharmacological properties. In vitro hairy root (HR) culture may serve as a promising solution for the extraction of tetrandrine, overcoming the limitations of natural cultivation. The present study describes the consistent production of tetrandrine from <em>S. tetrandra</em> hairy roots induced by different strains of <em>Agrobacterium rhizogenes</em>. Cultivation in woody plant medium (WPM) resulted in the highest HR biomass (0.056 g/petri-dish) and tetrandrine content (7.28 mg/L) as compared to other media. The maximum HR biomass (6.95 g dw/L) and tetrandrine production (68.69 mg/L) were obtained in the fifth week of cultivation. The presence of ammonium nitrate (800 mg/L), calcium nitrate (1156 mg/L), sucrose (20 g/L) and casein (2 g/L) enhanced the tetrandrine production. Moreover, the fed-batch cultivation demonstrated that the NH<sub>4</sub>NO<sub>3</sub> (1200 mg/L) was an important growth limiting factor that yielded the highest tetrandrine amount (119.59 mg/L). The cultivation of hairy roots in a mist trickling bioreactor for eight weeks was less (26.24 mg/L) than in the flask. Despite a lower tetrandrine yield observed in bioreactors compared to flask cultures, refining the growth medium and fine-tuning bioreactor operations hold promise for boosting tetrandrine yield.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141995830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endophyte-assisted non-host plant Tillandsia brachycaulos enhance indoor formaldehyde removal 内生菌辅助非宿主植物 Tillandsia brachycaulos 提高室内甲醛去除率。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-08-10 DOI: 10.1016/j.jbiotec.2024.07.022

{"title":"Endophyte-assisted non-host plant Tillandsia brachycaulos enhance indoor formaldehyde removal","authors":"","doi":"10.1016/j.jbiotec.2024.07.022","DOIUrl":"10.1016/j.jbiotec.2024.07.022","url":null,"abstract":"<div><p>This study investigated the use of endophyte-assisted <em>Tillandsia brachycaulos</em> to enhance formaldehyde removal in indoor environments. A formaldehyde-degrading endophyte from the root of <em>Epipremnum aureum</em>, <em>Pseudomonas plecoglossicida</em>, was identified and used for inoculation. Among the inoculation methods, spraying proved to be the most effective, resulting in a significant 35 % increase in formaldehyde removal after 36 hours. The results of the light exposure experiment (3000 Lux) demonstrate that an increase in light intensity reduces the efficiency of the <em>Tillandsia brachycaulos</em>-microbial system in degrading formaldehyde. In a 15-day formaldehyde fumigation experiment at 2 ppm in a normal indoor environment, the inoculated <em>Tillandsia brachycaulos</em> exhibited removal efficiency ranging from 42.53 % to 66.13 %, while the uninoculated declined from 31.62 % to 3.17 %. The <em>Pseudomonas plecoglossicida</em> (referred to as PP-1) became the predominant bacteria within the <em>Tillandsia brachycaulos</em> after fumigation. Moreover, the endophytic inoculation effectively increased the resistance and tolerance of <em>Tillandsia brachycaulos</em> to formaldehyde, as evidenced by lower levels of hydroxyl radical, malondialdehyde (MDA), free protein, and peroxidase activity (POD), as well as higher chlorophyll content compared to uninoculated <em>Tillandsia brachycaulos</em>. These findings indicate that the combination of endophytic bacteria and <em>Tillandsia brachycaulos</em> has significant potential for improving indoor air quality.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

System-wide analysis of groundnut's salinity resilience: Integrating plant-cell interactions with environmental stress dynamics through cutting-edge transcriptomics 花生抗盐碱能力的全系统分析：通过尖端转录组学整合植物-细胞相互作用与环境压力动态。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-08-10 DOI: 10.1016/j.jbiotec.2024.07.023

{"title":"System-wide analysis of groundnut's salinity resilience: Integrating plant-cell interactions with environmental stress dynamics through cutting-edge transcriptomics","authors":"","doi":"10.1016/j.jbiotec.2024.07.023","DOIUrl":"10.1016/j.jbiotec.2024.07.023","url":null,"abstract":"<div><p>Salinity stress is a major concern in regions where irrigation relies on saline water. This study aimed to investigate the relative water content (RWC), electrolytic leakage (EL), total chlorophyll content, free amino acid content, and total soluble sugar content were analyzed in different groundnut species subjected to various salinity treatments. The results showed that salinity stress significantly reduced the RWC in groundnut leaves, with <em>A. duranensis</em> (wild type) exhibiting higher RWC values compared to the <em>Arachis hypogaea</em> species. RNA sequencing was performed to identify differentially expressed genes (DEGs) during salt stress. A total of 9079 DEGs were identified, with 1372 genes upregulated and 2509 genes downregulated. Genes belonging to transcription factor families, such as WRKY, MYB, bHLH, E2F, and Auxin efflux carrier proteins, were induced under salt stress in the tolerant genotype. Conversely, genes encoding NADH dehydrogenase, glutathione S-transferase, protein kinases, UDP-glycosyltransferase, and peroxidase were downregulated. Gene ontology and pathway analyses revealed several enriched categories and metabolic pathways associated with salt stress response, including catalytic activity, response to salt stress, ATP-dependent activity, and oxidative phosphorylation. The findings of this study provide insights into the physiological and molecular responses of groundnut to salinity stress. <em>A. duranensis</em> exhibited better salinity tolerance than <em>Arachis hypogaea</em>, as indicated by higher RWC values, lower electrolytic leakage, and differential gene expression patterns. These results contribute to our understanding of the mechanisms underlying salt stress tolerance in groundnut and may guide future efforts to develop salinity-tolerant groundnut species, ultimately improving crop yield in saline-affected regions.</p></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":null,"pages":null},"PeriodicalIF":4.1,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141916758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0