Leaf vein scaffolds for three-dimensional culture of PDLSCs-derived Muse cells

IF 4.1 2区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal of biotechnology Pub Date : 2025-06-10 DOI:10.1016/j.jbiotec.2025.06.005

Xueting Bi , Qian Lan , Xin Xiao , Yu Deng , Dongsheng Li

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引用次数: 0

Abstract

Periodontal disease, a significant global health burden, has encountered limited success with current therapeutic strategies to achieve full tissue regeneration. The emergence of Multilineage Differentiation and Stress-Enduring (Muse) cells presents a promising avenue for periodontal tissue regeneration. This study introduced a novel three-dimensional (3D) culture system utilizing Magnolia leaf vein scaffolds, characterized for their biocompatibility and evaluated for their impact on Muse cells' proliferation, adhesion, osteogenic differentiation, and exosome secretion. The isolation of Muse cells from Periodontal Ligament Stem Cells (PDLSCs) was successfully accomplished, with excellent compatibility observed with the plant-derived scaffolds. Notably, the 3D culture substantially upregulated osteogenic markers and promoted the formation of mineralized nodules, signifying enhanced osteogenic potential. Additionally, Muse cells in 3D culture exhibited a significant increase in exosome secretion, which were more effective in stimulating PDLSCs proliferation. The study concluded that plant leaf vein scaffolds provide a sustainable and effective platform for 3D stem cell culture, with the potential to significantly enhance the therapeutic efficacy of Muse cells in periodontal tissue engineering.

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用于pdlscs来源的Muse细胞三维培养的叶静脉支架

牙周病是一个重大的全球健康负担，目前的治疗策略在实现完全组织再生方面取得了有限的成功。多谱系分化和应力持久（Muse）细胞的出现为牙周组织再生提供了一条有希望的途径。本研究介绍了一种利用木兰叶静脉支架的新型三维（3D）培养系统，以其生物相容性为特征，并评估了其对Muse细胞增殖、粘附、成骨分化和外泌体分泌的影响。从牙周韧带干细胞（Periodontal Ligament Stem cells, PDLSCs）中成功分离出Muse细胞，并与植物源性支架具有良好的相容性。值得注意的是，3D培养显著上调成骨标志物，促进矿化结节的形成，表明成骨潜力增强。此外，3D培养的Muse细胞外泌体分泌显著增加，更有效地刺激PDLSCs增殖。本研究认为植物叶静脉支架为三维干细胞培养提供了可持续有效的平台，具有显著提高Muse细胞在牙周组织工程中的治疗效果的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of biotechnology 工程技术-生物工程与应用微生物

CiteScore

8.90

自引率

2.40%

发文量

190

审稿时长

45 days

期刊介绍： The Journal of Biotechnology has an open access mirror journal, the Journal of Biotechnology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal provides a medium for the rapid publication of both full-length articles and short communications on novel and innovative aspects of biotechnology. The Journal will accept papers ranging from genetic or molecular biological positions to those covering biochemical, chemical or bioprocess engineering aspects as well as computer application of new software concepts, provided that in each case the material is directly relevant to biotechnological systems. Papers presenting information of a multidisciplinary nature that would not be suitable for publication in a journal devoted to a single discipline, are particularly welcome.