Journal of biotechnology最新文献

{"title":"Multilevel agro-physiological and biochemical alleviation of salt stress in Olea europaea via phenolic-rich extracts from olive mill waste","authors":"Samia Abboud ,&nbsp;Nada Ammar ,&nbsp;Azhar Ouni ,&nbsp;Mourad Jellali ,&nbsp;Darine Tlili ,&nbsp;Sahar Ben Abdelwaheb ,&nbsp;Amani Bchir ,&nbsp;Soumaya Dbara","doi":"10.1016/j.jbiotec.2026.01.013","DOIUrl":"10.1016/j.jbiotec.2026.01.013","url":null,"abstract":"<div><div>Soil salinization poses a critical threat to agricultural productivity, particularly in the Mediterranean regions where olive cultivation is of major economic and ecological significance. This study investigates the potential of phenolic extracts derived from olive mill solid waste (OMSW) to enhance salt stress tolerance in Olea europaea L. cv. Koroneiki. One-year-old olive plants were subjected to six treatments: a non-stressed control (C), phenolic extract application under non-stress conditions (C + PE-OMSW), OMSW amendment under non-stress conditions (C + OMSW), salt stress induced by 100 mM NaCl (SS), salt stress combined with phenolic extract (SS + PE-OMSW), and salt stress combined with OMSW (SS + OMSW). A comprehensive physiological and biochemical evaluation was conducted, including measurements of vegetative growth, relative water content, membrane stability, chlorophyll fluorescence, photosynthetic pigments, oxidative stress markers (malondialdehyde and hydrogen peroxide), osmoprotectants (proline and soluble sugars), total polyphenols, flavonoids, and leaf mineral content. Application of phenolic extract under saline conditions (SS + PE-OMSW) markedly improved plant performance by enhancing water status, preserving membrane integrity, and increasing chlorophyll fluorescence efficiency. These plants also exhibited higher polyphenols and flavonoids accumulation, along with significant reductions in oxidative stress markers, suggesting enhanced antioxidant defenses. Elevated levels of proline and soluble sugars further indicated an adaptive osmotic adjustment to salinity. These results demonstrate the efficacy of OMSW-derived phenolic extracts as sustainable biostimulants capable of mitigating salt stress through integrated physiological and biochemical mechanisms. This valorization pathway offers a promising approach to recycling agro-industrial residues into high-value agricultural inputs, contributing to climate-resilient and circular bioeconomy-based crop production systems.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"411 ","pages":"Pages 116-129"},"PeriodicalIF":3.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146074869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bispecific nanobodies – promising engineered candidates with high therapeutic efficiency","authors":"Sneha Banerjee,&nbsp;Sreeja Vangapally,&nbsp;Anna Mariya,&nbsp;Bhaskar Paidimuddala","doi":"10.1016/j.jbiotec.2026.01.010","DOIUrl":"10.1016/j.jbiotec.2026.01.010","url":null,"abstract":"<div><div>Bispecific nanobodies are the emerging class of engineered nanobodies with great scope for targeted therapy due to their small size, high stability, extended half-life, and dual antigenic recognition properties. They can bind to two different antigens simultaneously which significantly improves the therapeutic index in the treatment of various diseases. To achieve enhanced binding avidity, specificity and minimal off-target effects, the bispecific nanobodies have been further subjected to advanced engineering strategies such as site-specific combinations, fusions and multivalent formats. The recombinant production of bispecific nanobodies has been tested in bacterial, yeast and mammalian expression platforms to facilitate their large-scale production and cost-effective clinical applications. This review exclusively focuses on recent progress in the design and development of bispecific nanobodies. It presents the engineering of nanobodies into bispecific formats, their design, expression strategies and therapeutic outcomes. It also discusses the recent preclinical and clinical developments of bispecific nanobodies focusing on tackling half-life extension problems, reducing immunogenicity and optimal delivery modalities. Overall, with the promising scope of bispecific nanobodies as targeted therapies, their acceptance into mainstream medicine holds great promise for precision and personalized therapeutic approaches for the effective treatment of various infections, cancers, autoimmune and neurodegenerative diseases.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"411 ","pages":"Pages 102-115"},"PeriodicalIF":3.9,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146063078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production of high-nutrient protein sources by pre-secondary solid-state fermentation of functional microbiota (Pre-Sec-SSF-FM) from dehulled chinese distillers grains","authors":"Lian Zhang ,&nbsp;Xiaojuan Shen ,&nbsp;Zhi Wang ,&nbsp;Yuansong Peng ,&nbsp;Jianping Yang ,&nbsp;Yafan Cai ,&nbsp;Shilei Wang ,&nbsp;Jingliang Xu ,&nbsp;Hanjie Ying","doi":"10.1016/j.jbiotec.2025.11.012","DOIUrl":"10.1016/j.jbiotec.2025.11.012","url":null,"abstract":"<div><div>Chinese distillers grains (CDGs) are used as low-cost feed additives, because they have issues such as high crude fiber content (&gt; 24 %), low crude protein content (&lt; 17 %), and low digestibility (&lt; 16 %). This study aimed to evaluate whether pre-secondary solid-state fermentation using functional microbiota can improve protein digestibility and reduce fiber content in CDGs. Results indicated that compared to CDGs, both true and crude protein content in solid-state fermented DCDGs (SSF-DCDGs) showed an upward trend, reaching increases of over 24 %. Compared to DCDGs, crude fiber content decreased; dry matter and protein digestibility increased by 29.54 % and 54.57 % in SSF-DCDGs. Amino acid content rose by 14.92 %, with glycine and alanine increased by 15.36 % and 21.25 % respectively—both being important aromatic flavor compounds. Therefore, the Pre-Sec-SSF-FM technical route can convert low-cost CDGs into a high-nutritional protein sources, offering a novel approach for protein raw material sourcing.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"410 ","pages":"Pages 23-32"},"PeriodicalIF":3.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145587491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Halotolerant black yeast Neophaeotheca triangularis as a source of melanin","authors":"Mercedes Peña ,&nbsp;Patricia Gómez-Villegas ,&nbsp;Jose Prados ,&nbsp;Consolación Melguizo ,&nbsp;Carla C.C.R. de Carvalho","doi":"10.1016/j.jbiotec.2025.11.011","DOIUrl":"10.1016/j.jbiotec.2025.11.011","url":null,"abstract":"<div><div>Archaea and bacteria are the most studied extremophiles, but fungi also demonstrate remarkable tolerance, particularly in hypersaline environments such as solar salterns. Among salt-adapted fungi, black yeasts have been shown to be adapted to such environments, having developed defense mechanisms such as the production of melanin, a pigment that plays a crucial role in environmental stress protection. Melanin is a complex, high-molecular-weight polymer widely found across biological kingdoms. During the isolation of microorganisms from samples collected in crystallization ponds in a saltern, a black yeast was found able to produce significant amounts of melanin. The yeast was identified as belonging to the species <em>Neophaeotheca triangularis</em>. The aim of this study was to optimize the cultivation conditions of the strain, to characterize the produced melanin, and to assess its biological activities, including its antitumor and antioxidant properties.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"410 ","pages":"Pages 10-22"},"PeriodicalIF":3.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145523618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro synthesis of β-aspartyl-basic amino acid dipeptides via a multi-enzyme cascade system with ATP regeneration","authors":"Fei-yan Cheng ,&nbsp;Long-wei Lou ,&nbsp;Zong-lin Li ,&nbsp;Zhi-min Li","doi":"10.1016/j.jbiotec.2025.12.017","DOIUrl":"10.1016/j.jbiotec.2025.12.017","url":null,"abstract":"<div><div>Cyanophycin, also known as cyanophycin granule polypeptide (CGP), was a natural polyamide synthesized via a nonribosomal pathway from <span>L</span>-aspartic acid and <span>L</span>-arginine. Its derived dipeptide, β-Asp-Arg, holds potential applications in various fields requiring arginine supplementation in feed or food industries. However, conventional CGP production methods were constrained by strict substrate specificity and high costs. To address these challenges, two-step enzymatic cascade system was constructed integrating CGP biosynthesis with dipeptide production. Central to this system is a novel cyanophycin synthetase (CphA1) from <em>Acidobacteria bacterium</em>, which exhibits remarkable substrate promiscuity. This enzyme enables both primer-independent polymerization of poly-<span>L</span>-Arg/<span>L</span>-Lys-poly(<span>L</span>-Asp) backbones and primer-dependent incorporation of non-canonical substrates (<span>L</span>-ornithine and <span>L</span>-citrulline), thereby expanding the repertoire of cyanophycin derivatives. Furthermore, polyphosphate kinase synergizes with CphA1 to regenerate ATP and promote CGP synthesis, reducing ATP consumption by 90 %. Subsequently, the polymer was efficiently hydrolyzed by CphB from <em>Geminocystis herdmanii</em> to yield the target dipeptides. This method achieved complete conversion of CGP into classical dipeptides β-Asp-Arg and β-Asp-Lys, with the highest titer reaching 80 mM, while also generating non-classical products β-Asp-Orn and β-Asp-Cit. These results highlight the robustness and versatility of this strategy, offering a promising route for the scalable synthesis of β-Asp-basic amino acid dipeptides.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"410 ","pages":"Pages 276-284"},"PeriodicalIF":3.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145843647","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FRET two-hybrid assay-based target drug screening in living cells","authors":"Beini Sun ,&nbsp;Gengqiang Cao ,&nbsp;Tian Gan ,&nbsp;Yue Wang ,&nbsp;Rumeng Qu ,&nbsp;Lin Hu ,&nbsp;Yuan Chang ,&nbsp;Min Hu ,&nbsp;Xiaoping Wang ,&nbsp;Tongsheng Chen","doi":"10.1016/j.jbiotec.2025.11.010","DOIUrl":"10.1016/j.jbiotec.2025.11.010","url":null,"abstract":"<div><div>Precise targeted drug screening is the key to improve the efficiency of tumour targeted therapy. This report presents a live cell FRET two-hybrid assay-based targeted drug screening method (FRET-HBTDS). In FRET-HBTDS, the cells co-expressing donor- and acceptor-labelled targets were cultured in 96-well plates, quantitative FRET imaging was then performed on a self-built automated FRET microscope (FRETscope) well-by-well, and FRET two-hybrid assay was used to obtain the maximum donor-centre FRET efficiency (<em>E</em>_{<em>Dmax</em>}), maximum acceptor-centre FRET efficiency (<em>E</em>_{<em>Amax</em>}) and stoichiometric ratios (<em>N</em>_<em>A</em><em>/N</em>_<em>D</em>). The FRET-HBTDS method was performed on the FRETscope with a 20 × objective for the cells co-expressing CFP-Bcl-xL and YFP-Bak to assess the action of eight compounds (A1331852, S63845, AC, DSF/Cu, Met, REGO, SOFA, ABT199) on the interaction between Bcl-xL and Bak. After 7 h of treatment with these compounds respectively, only the A1331852 group showed significantly lower <em>E</em>_{<em>Dmax</em>} and <em>E</em>_{<em>Amax</em>} compared to the control group, and a significant increase in <em>N</em>_<em>A</em><em>/N</em>_<em>D</em>, suggesting that A1331852 unlocks the direct interaction between Bcl-xL and Bak, thus releasing Bak to induce cell death. In addition, the <em>N</em>_<em>A</em><em>/N</em>_<em>D</em> of the DSF/Cu group was significantly higher than of the control group, suggesting that DSF/Cu altered the stoichiometry of the Bcl-xL-Bak complex. Our data firmly demonstrate that A1331852 unlocks the binding state of Bcl-xL and Bak, while DSF/Cu modifies the structure of the Bcl-xL-Bak complex. These findings demonstrate that FRET-HBTDS can be used to assess the efficacy of a drug by revealing the binding state and complex molecular structure of the target proteins using FRET technology in living cells, which may be a potential targeted drug screening method.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"410 ","pages":"Pages 1-9"},"PeriodicalIF":3.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145523543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Experimental immobilization of Clostridium histolyticum collagenases on green-synthesized CuO nanoparticles supported by bioinformatics analysis","authors":"Farzaneh Barati ,&nbsp;Fakhrisadat Hosseini ,&nbsp;Parinaz Ghadam ,&nbsp;Seyed Shahriar Arab","doi":"10.1016/j.jbiotec.2025.11.019","DOIUrl":"10.1016/j.jbiotec.2025.11.019","url":null,"abstract":"<div><div>Enzymes are widely used in various industries due to their eco-friendly nature, high selectivity, and ability to operate under mild conditions. However, large-scale applications are often hindered by challenges such as limited stability, high costs, and difficulties in recyclability. Enzyme immobilization presents a promising strategy to overcome these limitations. Microbial collagenases, particularly those from <em>Clostridium histolyticum</em>, play a crucial role in industrial and biomedical applications. <em>In silico</em> analyses effectively predict enzyme immobilization outcomes, minimizing experimental time and cost. This study employed enzyme surface analysis, a bioinformatics approach, to determine the surface charge type of the support for the physical immobilization of <em>C. histolyticum</em> collagenases. The analysis identified the most suitable regions on the enzyme surfaces for attachment—positively charged and distant from critical sites—ensuring minimal disruption to activity. Copper oxide (CuO) nanoparticles (NPs) were synthesized using walnut green husk extract and subsequently characterized. The green-synthesized CuO NPs were monodisperse and spherical (∼40 nm) with a negatively charged surface, confirming their suitability as supports for enzyme immobilization. Employing the CuO NPs as supports resulted in an immobilization yield of 42.15 % and an activity yield of 146.2 %. Further optimization of immobilization conditions could improve IY. The high AY suggests that immobilization did not hinder enzyme function but may have enhanced enzyme-substrate affinity and increased local substrate concentration, thereby boosting enzyme efficiency.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"410 ","pages":"Pages 105-114"},"PeriodicalIF":3.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145654360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emerging strategies for enhanced glutathione biosynthesis and its biomedical applications","authors":"Yajie Wang ,&nbsp;Ruixue Ma ,&nbsp;Jia Zhou ,&nbsp;Zhongbiao Tan ,&nbsp;Hongliang Li ,&nbsp;Hao Shi ,&nbsp;Yegui Zhang","doi":"10.1016/j.jbiotec.2025.11.016","DOIUrl":"10.1016/j.jbiotec.2025.11.016","url":null,"abstract":"<div><div>Glutathione (GSH), a ubiquitous bioactive tripeptide, plays a central role in antioxidant defense, detoxification, and cellular metabolism. This review highlights advanced strategies to enhance GSH production, emphasizing strain improvement via traditional mutagenesis and genetic engineering. Optimization of microbial fermentation through carbon source selection, amino acid precursors, and ATP regeneration combined with metabolic engineering approaches, has significantly improved GSH yields in <em>Saccharomyces cerevisiae</em> and <em>Escherichia coli</em>. Clinically, GSH is used in managing liver diseases, oxidative stress, and immune dysfunction. Emerging applications, particularly in oncology, show promise. This review also compares production methods such as chemical synthesis, enzymatic catalysis, and microbial fermentation, highlighting microbial fermentation for its sustainability, cost-efficiency, and scalability. Future research should focus on refining detection methods, elucidating GSH’s therapeutic mechanisms, and broadening its applications across pharmaceuticals, nutrition, and cosmetics.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"410 ","pages":"Pages 45-56"},"PeriodicalIF":3.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145622161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Directional lactic acid production could mitigate antibiotic and ARGs pollution","authors":"Fubin Yin ,&nbsp;Tianjing Lian ,&nbsp;Haoying Qu ,&nbsp;Dongan Cui ,&nbsp;Xiaomeng Li ,&nbsp;Yangyang Cai ,&nbsp;Hongmin Dong","doi":"10.1016/j.jbiotec.2025.11.014","DOIUrl":"10.1016/j.jbiotec.2025.11.014","url":null,"abstract":"<div><div>The technology of directional lactic acid production (DLAP) for animal manure is an innovative way of value-added utilization, but the influence of residual antibiotics in animal manure on the DLAP is still unclear, especially the degradation rate and degradation pathways of antibiotic with residues lactic acid bacteria (LAB). In this study, the most common residual antibiotic (Sulfachloropyridazine, SCP) and LAB (<em>Lactobacillus bulgaricus, Lactobacillus acidophilus, Lactobacillus plantarum and Lactobacillus casei</em>) were employed as the research objects, the SCP degradation, degradation pathways and antibiotic resistance genes (ARGs) with different LAB were investigated in pure culture. Results showed that LAB has a positive effect on the reduction in ARGs (sul1, sul2, sul3, sulA, intI1 and tnp A). The SCP degradation rates were 90.3 %, 85.1 %, 86.1 % and 84.0 % with the growth of <em>Lactobacillus bulgaricus</em>, <em>Lactobacillus acidophilus</em>, <em>Lactobacillus plantarum</em> and <em>Lactobacillus casei</em> at 50 mg/L, respectively. And the SCP degradation pathway is different for LAB, but the main decomposition functions, including S-N bond cleavage, CH₃ release, SO₂ release, nitration, hydroxylation, NH₂ release and C-N bond cleavage, are similar. And most of intermediate products of SCP were lower toxicity compared to the parent compound. The findings proved the DLAP process as a guidance for a potential solution for reducing antibiotic and ARGs contamination.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"410 ","pages":"Pages 96-104"},"PeriodicalIF":3.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145622130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multi-parameter process optimization for high yield AAV2 vector production using scale-down multi-parallel bioreactor systems and high-throughput analytical tools","authors":"Nazgul Wagner,&nbsp;Jonas Austerjost,&nbsp;Julia Niemann","doi":"10.1016/j.jbiotec.2025.12.007","DOIUrl":"10.1016/j.jbiotec.2025.12.007","url":null,"abstract":"<div><div>Adeno-associated virus (AAV) vectors are recognized as highly efficient carriers for genetic material. Among the various manufacturing platforms, transient production is the most established one for AAV production. Despite this, the transient production is influenced by numerous variables that can significantly impact the outcome. In this study, we employed 15 ml multi-parallel scale-down bioreactor system to evaluate critical process parameters like cell densities and plasmid DNA concentration. Additionally, a 250 ml bioreactor system was used to explore factors, such as bioprocess mode, comparing batch versus perfusion processes under optimized conditions. Results showed that in low cell density AAV production, where cell specific productivity is highest, the perfusion process yielded over three-fold lower functional titers compared to the batch process at 37°C. Furthermore, the impact of temperature-shifts, a yield improvement strategy that is rarely explored in the context of AAV production, was examined, resulting in increased functional AAV yields - almost two-fold in batch process and over three-fold increase in perfusion process. The temperature-shift not only increased total number of functional AAV particles but also improved the ratio of functional to capsid titers, suggesting enhanced encapsidation or reduced genomic loss from filled particles. After identifying the optimal setup, the process was successfully scaled up to a benchtop bioreactor, demonstrating the scalability and reproducibility of the optimized process. Production results were rapidly assessed using high-throughput analytical techniques, evaluating capsid titers via the biolayer interferometry (BLI)-based Octet® platform and functional titers through an <em>in vitro</em> assay using the high-throughput imaging-based Incucyte® system.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"410 ","pages":"Pages 228-235"},"PeriodicalIF":3.9,"publicationDate":"2026-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145804653","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}