Journal of biotechnology最新文献_第3页

Customized design of host-independent T7 expression system (HITES) for a broad host range 针对广泛宿主范围的非宿主T7表达系统（HITES）的定制设计。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-01 DOI: 10.1016/j.jbiotec.2024.12.012

Mingxin Cui , Okei Wong , Kexin Shi , Qiang Li , Wenya Wang

{"title":"Customized design of host-independent T7 expression system (HITES) for a broad host range","authors":"Mingxin Cui , Okei Wong , Kexin Shi , Qiang Li , Wenya Wang","doi":"10.1016/j.jbiotec.2024.12.012","DOIUrl":"10.1016/j.jbiotec.2024.12.012","url":null,"abstract":"<div><div>Efficient methods and universal DNA elements are eagerly required for the expression of proteins and the production of target chemicals in synthetic biology and metabolic engineering. This paper develops a customized-design approach by utilizing the host-independent T7 expression system (HITES), which facilitates the rational design and rapid construction of T7 expression systems. Firstly, the E<sub>i</sub>L (Upper-limit value of initial enzyme activity) value is discovered to play a pivotal factor in the successful construction of the T7 expression system, different host strains exhibit varying E<sub>i</sub>L values, and this study presents a method to measure the E<sub>i</sub>L values. Secondly, <em>E. coli</em> DH5α is chosen as the host strain, and it demonstrates that various strategies to modulate T7 RNA polymerase activity can efficiently construct the HITES T7 expression system in <em>E. coli</em> DH5α under the guidance of E<sub>i</sub>L. Lastly, the customized-design of HITES enables the efficient expression of sfGFP and D-MIase proteins across 13 host strains, guided by E<sub>i</sub>L values. This customized-design method of HITES offers a streamlined pathway for T7 system construction across a broad range of hosts and serves as an enabling tool for synthetic biology, metabolic engineering, and enzyme engineering.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"398 ","pages":"Pages 202-214"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142949322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Engineering an Escherichia coli surface display platform based on an autotransporter from Stenotrophomonas maltophilia: Autodisplay of enzymes with low to high molecular weight 基于嗜麦芽寡养单胞菌的自转运体构建大肠杆菌表面展示平台：低分子量到高分子量酶的自动展示。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-01 DOI: 10.1016/j.jbiotec.2024.12.003

Kunpeng Gao , Kexin Yu , Jianan Sun , Xiangzhao Mao , Hao Dong

{"title":"Engineering an Escherichia coli surface display platform based on an autotransporter from Stenotrophomonas maltophilia: Autodisplay of enzymes with low to high molecular weight","authors":"Kunpeng Gao , Kexin Yu , Jianan Sun , Xiangzhao Mao , Hao Dong","doi":"10.1016/j.jbiotec.2024.12.003","DOIUrl":"10.1016/j.jbiotec.2024.12.003","url":null,"abstract":"<div><div>Surface display technology has garnered significant attention for preparing efficient whole cell catalysts, while reported carrier proteins still cannot meet the demand to display various passenger domains, especially for those with high molecular weight. This study demonstrates that the autotransporter of esterase Est7 (E7AT) from <em>Stenotrophomonas maltophilia</em> played a decisive role in its efficient surface display. Guided by the original signal peptide, the surface display ratio of Est7 was determined as 89.67 % with the total enzymatic activity of 16.67 U/mL, which was much higher than 4.60 U/mL and 5.70 U/mL for the signal peptides derived from pectolase B (pelB) and cholera toxin B (ctxB), respectively. Then, the E7AT unit was successfully developed to surface display proteins with varying molecular weight from 19.3 kDa to 117.9 kDa, showing a more effective autodisplay ability than adhesin involved in diffuse adherence (AIDA-I), ice nucleation proteins (InaK and InaP), and outer membrane proteins (lipoprotein/ompA, MltA-interacting protein A, and yiaT) systems. Additionally, a galactosidase (GAL) displayed by E7AT was employed to hydrolyze lactose, achieving a promising hydrolysis rate of 31.63 % in 2 h. The displayed GAL retained 63.24 % and 41.41 % activity in third and sixth batch, respectively, indicating the considerable potential of E7AT in developing efficient whole cell catalysts.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"398 ","pages":"Pages 97-104"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of an advanced acetaldehyde detection solution based on yeast and bacterial surface display technology 基于酵母和细菌表面显示技术的先进乙醛检测溶液的开发。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-01 DOI: 10.1016/j.jbiotec.2024.11.020

Weigeng Liu, Jiamin Cao, Di Wu, Yue Wu, Yi Qin, Yanlin Liu, Xixi Zhao, Yuyang Song

{"title":"Development of an advanced acetaldehyde detection solution based on yeast and bacterial surface display technology","authors":"Weigeng Liu, Jiamin Cao, Di Wu, Yue Wu, Yi Qin, Yanlin Liu, Xixi Zhao, Yuyang Song","doi":"10.1016/j.jbiotec.2024.11.020","DOIUrl":"10.1016/j.jbiotec.2024.11.020","url":null,"abstract":"<div><div>Acetaldehyde, a carcinogen widely present in various beverages and the natural environment, necessitates convenient and efficient detection methods. In this work, two different host strains were used to develop a sensitive, convenient, and efficient whole-cell optical biosensor for acetaldehyde detection. Acetaldehyde dehydrogenase (AldH) was displayed on the cell surface of <em>Saccharomyces cerevisiae</em> and <em>E. coli</em> using flocculin protein and the N-terminal ice nucleation protein (INP), respectively. The successful construction of yeast and bacteria surface display platforms was confirmed by laser scanning confocal microscopy. Then, the optimal AldH-display system for yeast and bacteria was confirmed. The optimum reaction conditions were determined by changing testing temperatures and pH values. The differences between the two display systems were compared. The highest whole-cell activities of yeast and bacteria under optimal conditions were 3.68 ± 0.07 U/mL/OD<sub>600</sub> for BY-S6G and 6.95 ± 0.04 U/mL/OD<sub>600</sub> for E-32-I<sub>r</sub>A. The strains with the best performance were chosen for the detection of acetaldehyde in wine and other beverage samples and showed substrate specificity and accuracy, in which the recovery rate ranged between 94.4 % and 110.1 %. The results demonstrated that the AldH surface display strains could be used as an optical biosensor to detect acetaldehyde in beverages and red wine.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"398 ","pages":"Pages 42-50"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769262","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An in vitro 3D spheroid model with liver steatosis and fibrosis on microwell arrays for drug efficacy evaluation

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-01-29 DOI: 10.1016/j.jbiotec.2025.01.019

Jiamin Chen , Ping Wang , Zhanpeng Li , Jieyi Wu , Fang Tang , Niao Yang , Bohong Cen , Cuiyin Xie , Yufan Yang , Ziyan Yang , Chuwen Zhang , Xiangcao Yao , Zhongyuan Xu

{"title":"An in vitro 3D spheroid model with liver steatosis and fibrosis on microwell arrays for drug efficacy evaluation","authors":"Jiamin Chen , Ping Wang , Zhanpeng Li , Jieyi Wu , Fang Tang , Niao Yang , Bohong Cen , Cuiyin Xie , Yufan Yang , Ziyan Yang , Chuwen Zhang , Xiangcao Yao , Zhongyuan Xu","doi":"10.1016/j.jbiotec.2025.01.019","DOIUrl":"10.1016/j.jbiotec.2025.01.019","url":null,"abstract":"<div><div>Metabolic dysfunction-associated steatotic liver disease (MASLD) is now the most common chronic liver disease worldwide, affecting more than 30 percent of adults. The most severe form of MASLD, metabolic dysfunction-associated steatohepatitis (MASH), is characterized by necrotizing inflammation and rapid fibrosis progression, often leading to cirrhosis and hepatocellular carcinoma. Currently, only Resmetirom is approved for the treatment of MASH one of the main reasons is the absence of representative in vivo or in vitro models for MASH. To address this challenge, we developed a high-throughput 3D spheroid model consisting of human hepatocellular carcinoma cells (HepG2) and human hepatic stellate cells (LX-2) on microwell arrays. This model, induced with free fatty acids (FFA) to simulate steatosis and fibrosis, enables the assessment of efficacy and mechanisms for potential anti-MASH drugs. Our findings demonstrate that this in vitro spheroid model replicates key pathological features of human MASLD, including steatosis, oxidative stress, and fibrosis. Upon validation, we selected pirfenidone (PFD) and yinfenidone (AC-003), which are commonly used to treat idiopathic pulmonary fibrosis (IPF), to test their anti-MASH efficacy. Treatment with these drugs showed that they could regulate lipid synthesis and metabolism genes, reduce lipid accumulation, oxidative stress, and fibrosis levels. This 3D spheroid model represents a straightforward and efficient tool for screening anti-MASH drugs and investigating the molecular mechanisms of drug action.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"399 ","pages":"Pages 153-163"},"PeriodicalIF":4.1,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143074341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bifunctional chimeras of myeloperoxidase and glucose oxidase. Antimicrobial, topological and enzymatic properties

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-01-28 DOI: 10.1016/j.jbiotec.2025.01.018

Parfait Kenfack Ymbe , Claire Céré , Brigitte Delord , Gilles Pecastaings , Isabelle Ly , Aurélien Thureau , Laura Rodriguez , Zoran Ivanovic , Véronique Schmitt , Xavier Lafarge , Jean-Paul Chapel , Claire Stines-Chaumeil

{"title":"Bifunctional chimeras of myeloperoxidase and glucose oxidase. Antimicrobial, topological and enzymatic properties","authors":"Parfait Kenfack Ymbe , Claire Céré , Brigitte Delord , Gilles Pecastaings , Isabelle Ly , Aurélien Thureau , Laura Rodriguez , Zoran Ivanovic , Véronique Schmitt , Xavier Lafarge , Jean-Paul Chapel , Claire Stines-Chaumeil","doi":"10.1016/j.jbiotec.2025.01.018","DOIUrl":"10.1016/j.jbiotec.2025.01.018","url":null,"abstract":"<div><div>Enhancing the local substrate concentration is a crucial strategy in nature for facilitating the proximity of two enzymes. The substrate of the first enzyme is transformed into a by-product that travels to the active site of the second enzyme without external diffusion, then transformed into a product and eventually expelled from the complex. In an effort to optimize the antimicrobial properties of myeloperoxidase from <em>Rhodopirellula baltica</em> (RbMPO), we created a library of fused chimeras between a glucose oxidase (GOx) and RbMPO so that H<sub>2</sub>O<sub>2</sub> could be continuously perfused in the vicinity RbMPO, enabling the production of HOCl or HOSCN, well-known antimicrobial agents. The enzymes were characterized biochemically, enzymatically, and physically using low-resolution techniques such as AFM, SAXS, and cryofracture. SAXS experiments revealed that the chimeras were properly folded and existed in different oligomeric states. The kinetic parameters of the chimeras were determined and used for classification, revealing that all chimeras exhibited varying levels of activity and were microbicidal. The mixture of different oligomeric states of LEGGEAEA displayed both activity and microbicidal properties. AFM was used to visualize the chimeras in different oligomeric states, with their overall shapes ranging from round, oblong, to hooked, depending on the linker used.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"399 ","pages":"Pages 127-140"},"PeriodicalIF":4.1,"publicationDate":"2025-01-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient spermidine production using a multi-enzyme cascade system utilizing methionine adenosyltransferase from Lactobacillus fermentum with Reduced Product Inhibition and Acidic pH Preference

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-01-27 DOI: 10.1016/j.jbiotec.2025.01.016

Linbo Gou , Di Liu , Tai-ping Fan , Huaxiang Deng , Yujie Cai

{"title":"Efficient spermidine production using a multi-enzyme cascade system utilizing methionine adenosyltransferase from Lactobacillus fermentum with Reduced Product Inhibition and Acidic pH Preference","authors":"Linbo Gou , Di Liu , Tai-ping Fan , Huaxiang Deng , Yujie Cai","doi":"10.1016/j.jbiotec.2025.01.016","DOIUrl":"10.1016/j.jbiotec.2025.01.016","url":null,"abstract":"<div><div>Methionine adenosyltransferases (MATs; EC 2.5.1.6) are key enzymes that catalyze a crucial step in the spermidine biosynthesis pathway. Due to MAT's significant product inhibition, S-adenosylmethionine (SAM) and spermidine production faces challenges. We evaluated MATs from 20 lactic acid bacteria (LAB) to identify enzymes with acidic preference and lower susceptibility to product inhibition. <em>Lactobacillus fermentum</em>'s MAT (LfMAT) emerged as a candidate with desirable characteristics. LfMAT exhibited strong activity in acidic environments, maintaining over 85 % activity between pH 6.0–8.5 for 60 min, with peak efficacy at pH 7.0. LfMAT produced 4.2 mM SAM from 5 mM substrate, indicating reduced product inhibition. Ultimately, using an <em>in vitro</em> multi-enzyme cascade system containing LfMAT, S-adenosylmethionine decarboxylase, and spermidine synthase, we successfully produced 12.9 g·L<sup>−1</sup> of spermidine. This study establishes a cascade reaction platform, offering a novel approach for the efficient synthesis of spermidine and other polyamines.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"399 ","pages":"Pages 141-152"},"PeriodicalIF":4.1,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced methanol-xylose co-utilization strategy in Komagataella phaffii

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-01-26 DOI: 10.1016/j.jbiotec.2025.01.017

Kang Li , Shaojie Yang , Tengfei Wang , Chunjun Zhan , Zhonghu Bai , Yankun Yang

{"title":"Enhanced methanol-xylose co-utilization strategy in Komagataella phaffii","authors":"Kang Li , Shaojie Yang , Tengfei Wang , Chunjun Zhan , Zhonghu Bai , Yankun Yang","doi":"10.1016/j.jbiotec.2025.01.017","DOIUrl":"10.1016/j.jbiotec.2025.01.017","url":null,"abstract":"<div><div>Bio-manufacturing based on non-food carbon sources is conducive to alleviating the global food crisis and greenhouse effect. However, the mechanism of the utilization of methanol and xylose in <em>Komagataella phaffii</em> based on endogenous metabolic pathways has not been fully explored. In this study, transcriptomics revealed a positive correlation between methanol metabolic efficiency and the transcription level of genes related to xylose metabolism and phosphate metabolism. By providing sufficient phosphate to the strain, the methanol utilization rate of the <em>Komagataella phaffii</em> GA01 strain was improved, and the final biomass reached 7.5 g DCW/L. Metabolomics further confirmed that methanol could effectively activate xylose metabolism of the strain, and the consumption rates of methanol and xylose of the <em>Komagataella phaffii</em> GA01 strain could reach 3.87 g/L/d and 1.83 g/L/d, which were 34 % and 357.5 % higher than that of the wild-type strain, respectively. This study further promotes the application of methanol and xylose in microbial fermentation.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"399 ","pages":"Pages 117-126"},"PeriodicalIF":4.1,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143059120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction and characterization of a mutant library for the P23 constitutive promoter in lactic acid bacteria

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-01-21 DOI: 10.1016/j.jbiotec.2025.01.015

Linbing Yu , Xin Song , Guangqiang Wang , Yongjun Xia , Zibo Song , Gong Chen , Lianzhong Ai , Zhiqiang Xiong

{"title":"Construction and characterization of a mutant library for the P23 constitutive promoter in lactic acid bacteria","authors":"Linbing Yu , Xin Song , Guangqiang Wang , Yongjun Xia , Zibo Song , Gong Chen , Lianzhong Ai , Zhiqiang Xiong","doi":"10.1016/j.jbiotec.2025.01.015","DOIUrl":"10.1016/j.jbiotec.2025.01.015","url":null,"abstract":"<div><div>Promoters are crucial elements for controlling gene expression in cells, yet lactic acid bacteria (LAB) often lack a diverse set of available constitutive promoters with quantitative characterization. To enrich the LAB promoter library, this study focused on the known strong constitutive promoter P<sub>23</sub> in LAB. Through error-prone PCR and dNTP analog-induced random mutagenesis, a library of 247 mutants of P<sub>23</sub> was generated by using the red fluorescent protein (RFP) fluorescence intensity as a high-throughput screening indicator in <em>Streptococcus thermophilus</em>. The activity of P<sub>23</sub> mutants varied from 0.01 to 3.63 times that of P<sub>23</sub>. Similar trends of promoter strength were observed in <em>Lactobacillus plantarum</em> and <em>Lactococcus lactis</em>, but significant differences in <em>Escherichia coli</em>, indicating the library's specificity to LAB. To assess the application potential of this P<sub>23</sub> library, seven promoters with different strengths (0.28–2.58) were selected. The mutant promoters significantly enhanced the enzyme activities of superoxide dismutase (SOD), β-glucuronidase (GusA), and β-galactosidase (β-gal) in <em>S. thermophilus</em>. Notably, the mutant P<sub>23–203</sub> expressing SOD exhibited an enzyme activity of 382.9 U/mg, which was 1.65 times higher than the control (P<sub>23</sub>). Similarly, the expression of GusA and β-gal were 1.82 and 1.28 times higher than those of P<sub>23</sub>, respectively. This study provided a set of significantly different P<sub>23</sub> constitutive promoter mutant elements for the first time, laying the foundation for metabolic engineering and synthetic biology applications in LAB.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"399 ","pages":"Pages 99-107"},"PeriodicalIF":4.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancement of the production of terpenoid and flavonoid secondary metabolites in the ground and aerial parts of licorice composite plant in a hydroponic system

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-01-21 DOI: 10.1016/j.jbiotec.2025.01.014

Behrooz Mohammadparast , Zahra Shirazi

{"title":"Enhancement of the production of terpenoid and flavonoid secondary metabolites in the ground and aerial parts of licorice composite plant in a hydroponic system","authors":"Behrooz Mohammadparast , Zahra Shirazi","doi":"10.1016/j.jbiotec.2025.01.014","DOIUrl":"10.1016/j.jbiotec.2025.01.014","url":null,"abstract":"<div><div>Hairy roots mediated by <em>Agrobacterium rhizogenes</em> can be obtained from the composite plants (plants with hairy roots and untransformed aerial parts) by <em>ex vitro</em> method. Composite plants can produce higher amounts of secondary metabolites by merging hydroponic systems. This provides a stable condition for composite plants, in which various metabolites are produced in different parts. In the present study, composite plants of <em>Glycyrrhiza glabra</em> were produced under <em>ex vitro</em> conditions and transferred into a hydroponic culture medium to produce and extract secondary metabolites. The results showed a 4.8- and 1.8-fold enhancement in the expressions of <em>SQS1</em> and <em>bAS</em> genes in the roots of the composite plants compared to the control group, respectively. The levels of glycyrrhizin (1.7) and glabridin (3.5) were higher in the leaves of the composite plants compared to the controls. Moreover, higher amounts of glabridin (1.7) were observed in the roots of the composite plants compared to the control group. Investigation of the oxidative enzymes in the composite plants and control group revealed that the plants used more secondary metabolites through <em>Agrobacterium</em> inoculation. The plants needed more antioxidant enzymes to counter the release of oxygen-free radicals in control conditions, but composite plants used secondary metabolites as scavengers. Data revealed that composite plants managed to produce high amounts of various secondary metabolites in a hydroponic system.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"399 ","pages":"Pages 164-171"},"PeriodicalIF":4.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143028623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hemp tea waste-immobilized lipase for the synthesis of alkyl oleates in solvent free systems

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-01-20 DOI: 10.1016/j.jbiotec.2025.01.013

Viviana Chiappini, Debora Casbarra, Anna Maria Girelli

{"title":"Hemp tea waste-immobilized lipase for the synthesis of alkyl oleates in solvent free systems","authors":"Viviana Chiappini, Debora Casbarra, Anna Maria Girelli","doi":"10.1016/j.jbiotec.2025.01.013","DOIUrl":"10.1016/j.jbiotec.2025.01.013","url":null,"abstract":"<div><div>This study explores the immobilization of lipase from <em>Candida rugosa</em> (CRL) on hemp tea waste to catalyze the esterification of oleic acid with primary aliphatic C2-C12 alcohols in a solvent-free system. The immobilization method employed was adsorption, chosen for its simplicity, low cost, and ability to preserve enzyme activity. The esterification of undecanoic acid, lauric acid, and oleic acid with alcohols of varying chain lengths (ethanol, 1-propanol, 1-butanol, 1-octanol, 1-decanol, and 1-dodecanol) was studied. The esterification efficiency was found to be influenced by the type of alcohol, the molar ratio of oleic acid to alcohol, and the water content in the reaction medium. The highest conversions were achieved with ethanol (23 % conversion with 18.5 % water and a molar ratio of 1:5) and 1-decanol (30 % conversion with no added water and a molar ratio of 1:2). Comparatively, the soluble lipase achieved slightly higher conversion of oleic acid using decanol (38 %), indicating that the performance may be attributed to origin of lipase, rather than the immobilization procedure. The study demonstrated that the immobilization of lipase on hemp tea waste did not hinder enzyme activity. Additionally, the biocatalyst developed can function in a completely solvent-free system, offering a green solution by repurposing waste materials for industrial ester production.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"399 ","pages":"Pages 108-116"},"PeriodicalIF":4.1,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143023578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0