Journal of biotechnology最新文献

{"title":"Fabrication of a sensitive neurotransmitter detecting amperometric biosensor employing laccase nanoparticles on a pencil graphite electrode","authors":"Himani Guliya ,&nbsp;Suman Lata ,&nbsp;Reeti Chaudhary","doi":"10.1016/j.jbiotec.2025.04.021","DOIUrl":"10.1016/j.jbiotec.2025.04.021","url":null,"abstract":"<div><div>Neurotransmitters including dopamine, adrenaline, and noradrenaline are members of the important class of biogenic amines known as catecholamines. They perform crucial roles in various physiological processes and are frequently associated with stress responses, neurodegenerative and cardiovascular diseases, including Parkinson's and Alzheimer's. This research presents the fabrication of a novel amperometric biosensor designed to detect catecholamine levels with high specificity and sensitivity. The fabrication of the biosensor is based on the immobilization of synthesized cysteine functionalized laccase nanoparticles (Lac-NPs) onto the pencil graphite electrode (PGE). The successful synthesis of Lac-NPs, along with their immobilization and the fabrication of the Lac-NPs/PGE biosensor, was validated through various techniques, including Transmission Electron Microscopy (TEM), Fourier Transform Infrared Spectrophotometer (FTIR), UV–visible Spectroscopy, Dynamic Light Scattering (DLS), Zeta potential, Scanning Electron Microscope (SEM), Cyclic Voltammetry (CV) and Electrochemical Impedance Spectroscopy (EIS). The biosensor was optimized at various pH, temperature, scan rate and response times to ensure a high-performance sensor with rapid response times and stability for better detection. The Lac-NPs/PGE biosensor showed high sensitivity (2320.0 µA/mM cm²), a lower limit of detection (LOD) (0.12 µM), and a broad linear range (0.1–800.0 µM) with a coefficient of determination of R²= 0.999. In the analysis of real pharmaceutical samples of neurotransmitters, high recovery rates (94.0–99.0 %) have been attained. Superior analytical performance resulting from this simple fabrication process and cost-effective PGE shows this biosensor is a promising tool for the accurate and real-time monitoring of catecholamine levels, with potential applications in clinical diagnostics, neurobiology, and environmental analysis.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"404 ","pages":"Pages 152-161"},"PeriodicalIF":4.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143891008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biotechnological approaches for the production of neuroactive huperzine A","authors":"Mubeen Fatima ,&nbsp;Phetcharat Boonruamkaew ,&nbsp;Mengquan Yang ,&nbsp;Amit Jaisi","doi":"10.1016/j.jbiotec.2025.04.020","DOIUrl":"10.1016/j.jbiotec.2025.04.020","url":null,"abstract":"<div><div>Huperzine A (HupA), a natural Lycopodium alkaloid primarily derived from <em>Huperzia serrata</em>, has gained attention for its potent neuroprotective properties, particularly its ability to inhibit acetylcholinesterase and modulate key neurological pathways. This review highlights HupA’s therapeutic potential in managing neurodegenerative disorders such as Alzheimer’s disease, Parkinson’s disease, Huntington’s disease, Multiple sclerosis, Epilepsy, and Myasthenia gravis drawing on a comprehensive literature survey of <em>in vitro</em>, <em>in vivo</em>, and clinical investigation data. Given the limited yield from natural sources, this review also focuses on biotechnological strategies to enhance HupA production. These include chemical synthesis, microbial fermentation using endophytic fungi, plant tissue culture, and emerging synthetic biology approaches. Key biosynthetic intermediates and enzymes, such as lysine decarboxylase, copper amine oxidase, and cytochrome P450s, are discussed in the context of metabolic pathway elucidation and engineering. The review emphasizes the need to bridge current knowledge gaps in HupA biosynthesis to develop cost-effective, sustainable production methods. Advances in metabolic pathway elucidation and engineering hold immense potential for scalable biosynthetic production. Ultimately, the integration of HupA into neurotherapeutic regimens, coupled with innovations in its production, could revolutionize the management of neurodegenerative disorders and position it as a cornerstone of future multi-targeted treatment strategies.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"404 ","pages":"Pages 186-198"},"PeriodicalIF":4.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism of metabolites distribution between 2,3-butanediol and branched-chain amino acid synthesis pathways in Klebsiella pneumoniae","authors":"Yaoyu Cai ,&nbsp;Weiyan Jiang ,&nbsp;Qinghui Wang ,&nbsp;Shaoqi Sun ,&nbsp;Wenqi Wang ,&nbsp;Xinjie Bian ,&nbsp;Taiyu Liu ,&nbsp;Marina Tišma ,&nbsp;Dexin Wang ,&nbsp;Jian Hao","doi":"10.1016/j.jbiotec.2025.04.018","DOIUrl":"10.1016/j.jbiotec.2025.04.018","url":null,"abstract":"<div><div><em>Klebsiella pneumoniae</em> is a commonly known 2,3-butanediol producer. 2,3-Butanediol synthesis and branched-chain amino acid (BCAA) synthesis pathways share the same step of α-acetolactate synthesis from pyruvate. Those two pathways do not interfere with each other in the wild-type strain. Knocking out <em>budA</em> (encoding α-acetolactate decarboxylase) blocks the 2,3-butanediol synthesis pathway. Meanwhile, metabolites of the BCAA synthesis pathway (valine, 2-ketoisovalerate, 2,3-dihydroxyisovalerate and 2-hydroxyisovalerate) are accumulated. However, the mechanism underlying the metabolite changes resulting from the inactivation of <em>budA</em> remains unclear. In this study, both <em>ex vivo</em> and <em>in vitro</em> experiments were conducted to elucidate this mechanism. Kinetic parameters of BudA and acetohydroxy acid isomeroreductase (IlvC) were determined. BudA has a higher affinity toward α-acetolactate and has a higher catalytic constant (<em>K</em>_m = 3.66 mM, <em>k</em>_cat = 7.8 s⁻¹) compared to IlvC (<em>K</em>_m = 17.98 mM, <em>k</em>_cat = 0.68 s⁻¹). <em>ex vivo</em> experiments showed that IlvC activities were not influenced by knocking out <em>budA</em> and vice versa. IlvC activities were improved in the cells in which <em>ilvC</em> was overexpressed, but this did not lead to the accumulation of metabolites of the BCAA synthesis pathway. The activities of IlvC in the cell were not affected by the accumulation of 2,3-dihydroxyisovalerate, 2-ketoisovalerate, or valine in the broth. These results indicated that the competitiveness of BudA and IlvC in the cell determines the metabolites distribution between those two pathways. The inactivation of BudA and intact IlvC led to the exceeded α-acetolactate flow into the BCAA synthesis pathway, which caused the accumulation of metabolites of the BCAA synthesis pathway.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"404 ","pages":"Pages 175-185"},"PeriodicalIF":4.1,"publicationDate":"2025-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143899766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances in photonic crystal hydrogels for biomedical research: A review","authors":"Jingtian Geng ,&nbsp;Kan Shao ,&nbsp;Peng Zhang ,&nbsp;Cheng Chen ,&nbsp;Shan Huang","doi":"10.1016/j.jbiotec.2025.04.019","DOIUrl":"10.1016/j.jbiotec.2025.04.019","url":null,"abstract":"<div><div>Photonic crystal hydrogel is an advanced material that combines the properties of photonic crystal and hydrogel with unique responsiveness and optical properties. By combining photonic crystals with hydrogels, the new material not only inherits the optical properties of photonic crystals, but also possesses the responsiveness and good biocompatibility of hydrogels. This makes the photonic crystal hydrogel show a broad application prospect in the field of sensors, clinical detection and biomedicine. In recent years, the use of photonic crystal hydrogels for sensors has been studied and applied. By designing different photonic crystal hydrogel materials, the temperature, humidity, and ion concentration in the environment can be targeted for highly sensitive detection. The results of the detection will be fed back in real time as visualized optical signals, i.e., changes in structural color. The material has been little studied in the clinical detection and biomedical fields and deserves further exploration. In this review, we first introduce the basic information of photonic crystal hydrogels, such as the structure and synthesis of the material. Then, we further describe the current research progress and preliminary results of this material in the fields of biosensing, drug release and clinical detection.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"404 ","pages":"Pages 162-174"},"PeriodicalIF":4.1,"publicationDate":"2025-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143894929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Succinic acid treatment enhances energy metabolism and antioxidant biosynthesis in radish sprouts","authors":"Tomasz Piechowiak,&nbsp;Maciej Balawejder","doi":"10.1016/j.jbiotec.2025.04.017","DOIUrl":"10.1016/j.jbiotec.2025.04.017","url":null,"abstract":"<div><div>Succinic acid is a molecule with strong biological activity, whose primary role in eucaryotic cells is to participate in the Krebs cycle and maintain normal energy metabolism. In the present study, we aimed to test whether the application of succinic acid into radish sprout cultures would stimulate antioxidant production by improving the energy metabolism. Our results demonstrated that succinic acid enhanced mitochondrial activity by upregulating the expression of TCA enzymes and oxidative phosphorylation pathway. This leads to both increased biosynthesis of ATP and elevated production of reactive oxygen species (ROS). The ROS generated subsequently activate cellular defense mechanism against oxidative stress, resulting in upregulated expression of antioxidant enzymes and enhanced biosynthesis of antioxidants. However, the effect of succinate on antioxidant levels was dependent on its concentration in the water. The highest concentration (10 mM) resulted in sprouts with the greatest levels of ascorbate and glutathione, whereas the lowest concentration (0.1 mM) stimulated the production of phenolic compounds.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"404 ","pages":"Pages 144-151"},"PeriodicalIF":4.1,"publicationDate":"2025-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143882461","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fragmentation of recombinant human interleukin-12 by matriptase in CHO cell culture","authors":"Fnu Aapjeet ,&nbsp;Tiffany Tang ,&nbsp;Yixiao Zhang ,&nbsp;Aditya Gopalan ,&nbsp;Satish Kallappagoudar ,&nbsp;Jessica Pan ,&nbsp;Fengfei Ma ,&nbsp;Sunil S. Shah ,&nbsp;Shannon Rivera ,&nbsp;Anita Ping-wen Liu ,&nbsp;Veronica Juan ,&nbsp;Ren Liu","doi":"10.1016/j.jbiotec.2025.04.010","DOIUrl":"10.1016/j.jbiotec.2025.04.010","url":null,"abstract":"<div><div>During the development of a recombinant CHO cell line expressing human Interleukin-12 fused to human IgG1 Fc (rhIL-12), we observed a prominent proteolytic cleavage of the rhIL-12 in its p40 subunit between Lys260 and Arg261. Using class-specific protease inhibitors, we concluded that the serine hydrolase family was responsible for the clipping. To identify the specific serine proteases involved, we conducted transcriptomic and proteomic analyses and identified several potential candidates. By performing in-vitro enzyme digestion experiments with these proteases, we determined that matriptase was responsible for the observed p40 clipping. Further confirmation was obtained through the development of matriptase (S<em>t14</em>) knockout cell lines in which rhIL-12 clipping was almost completely abolished. Armed with this knowledge, we devised several strategies including increasing culture pH to reduce matriptase activity and rhIL-12 clipping during the manufacturing process.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"404 ","pages":"Pages 112-120"},"PeriodicalIF":4.1,"publicationDate":"2025-04-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineering adaptive alleles for Escherichia coli growth on sucrose using the EasyGuide CRISPR system","authors":"Joneclei Alves Barreto ,&nbsp;Matheus Victor Maso Lacôrte e Silva ,&nbsp;Danieli Canaver Marin ,&nbsp;Michel Brienzo ,&nbsp;Ana Paula Jacobus ,&nbsp;Jonas Contiero ,&nbsp;Jeferson Gross","doi":"10.1016/j.jbiotec.2025.04.016","DOIUrl":"10.1016/j.jbiotec.2025.04.016","url":null,"abstract":"<div><div>Adaptive Laboratory Evolution (ALE) is a powerful approach for mining genetic data to engineer industrial microorganisms. This evolution-informed design requires robust genetic tools to incorporate the discovered alleles into target strains. Here, we introduce the EasyGuide CRISPR, a five-plasmid platform that exploits <em>E. coli</em>’s natural recombination system to assemble gRNA plasmids from overlapping PCR fragments. The production of gRNAs and donor DNA is further facilitated by using recombination cassettes generated through PCR with 40–60-mer oligos. With the new CRISPR toolkit, we constructed 22 gene edits in <em>E. coli</em> DH5α, most of which corresponded to alleles mapped in <em>E. coli</em> DH5α and E2348/69 ALE populations selected for sucrose propagation. For DH5α ALE, sucrose consumption was supported by the <em>cscBKA</em> operon expression from a high-copy plasmid. During ALE, plasmid integration into the chromosome, or its copy number reduction due to the <em>pcnB</em> deletion, conferred a 30–35 % fitness gain, as demonstrated by CRISPR-engineered strains. A ∼5 % advantage was also associated with a ∼40.4 kb deletion involving <em>fli</em> operons for flagella assembly. In E2348/69 ALE, inactivation of the <em>hfl</em> system suggested selection pressures for maintaining λ-prophage dormancy (lysogeny). We further enhanced our CRISPR toolkit using yeast for in vivo assembly of donors and expression cassettes, enabling the establishment of polyhydroxybutyrate synthesis from sucrose. Overall, our study highlights the importance of combining ALE with streamlined CRISPR-mediated allele editing to advance microbial production using cost-effective carbon sources.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"403 ","pages":"Pages 126-139"},"PeriodicalIF":4.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Glutamate-related nitrogen metabolism regulates cold-adaptive synthesis of red pigment in polar fungus Geomyces sp. WNF-15A","authors":"Jiawei Zhou ,&nbsp;Haoyu Long ,&nbsp;Yan Guo ,&nbsp;Jian Lu ,&nbsp;Nengfei Wang ,&nbsp;Haifeng Liu ,&nbsp;Xiangshan Zhou ,&nbsp;Menghao Cai","doi":"10.1016/j.jbiotec.2025.04.014","DOIUrl":"10.1016/j.jbiotec.2025.04.014","url":null,"abstract":"<div><div>The polar fungus <em>Geomyces</em> sp. WNF-15A produces high-quality red pigment (AGRP), but the cold-dependent characteristic of AGRP synthesis restricts its industrialization. This study employed transcriptome analysis to compare gene expression profiles of the wild-type strain with cold-independent mutants of <em>scaffold1.t692</em> (Δ1–692) and <em>scaffold2.t704</em> (Δ2–704). From the analysis, 23 candidate genes were identified and functionally characterized among 22,600 differentially expressed genes. Knockout and recovery of <em>scaffold5.t61</em>, <em>scaffold7.t586</em>, or <em>scaffold7.t712</em> proved their regulatory functions in AGRP synthesis, among which <em>scaffold5.t61</em> functioned as a transcription factor, while <em>scaffold7.t586</em> and <em>scaffold7.t712</em> were involved in the glutamate-related nitrogen metabolism. Exogenous addition of nitrate, glutamine, and glutamate, combined with transcriptional regulation studies, revealed the importance of glutamate metabolism for cold-adaptive synthesis of AGRP. <em>Scaffold5.t61</em> responded to the cold environment and regulated the transcription of <em>scaffold2.t704</em> and <em>scaffold1.t692</em>. It subsequently increased glutamate synthesis by regulating the key nitrogen metabolism genes of <em>scaffold7.t586</em> and <em>scaffold7.t712</em>, ultimately resulting in cold-dependent synthesis of AGRP in <em>Geomyces</em> sp. WNF-15A. This study offers new insights into the mechanisms of cold adaptation in polar fungi and serves as a reference for the development of psychrophilic fungal resources.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"404 ","pages":"Pages 121-131"},"PeriodicalIF":4.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143864102","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioprocessing of pineapple leaf waste biomass using an integrated ultrasound-deep eutectic solvent pretreatment approach for improved bioethanol production","authors":"Vishal Sharma ,&nbsp;Jia Xiang Wang ,&nbsp;Mei-Ling Tsai ,&nbsp;Aditya Yadav ,&nbsp;Cheng-Di Dong ,&nbsp;Parushi Nargotra ,&nbsp;Pei-Pei Sun","doi":"10.1016/j.jbiotec.2025.04.011","DOIUrl":"10.1016/j.jbiotec.2025.04.011","url":null,"abstract":"<div><div>Biorefineries play a crucial role in advancing the circular bioeconomy by integrating the environmental and socio-economic dimensions of the industrial sector. This study investigated the potential of integrated ultrasound (UL)-deep eutectic solvent (DES, choline chloride/glycerol) pretreatment of pineapple leaf (PL) waste for efficient bioethanol production, emphasizing its sustainability and environmental benefits. The pretreatment conditions were optimized using response surface methodology, with variables including ultrasound amplitude (45 %), time (30 min), and solid loading (10 %, w/w). The solid PL biomass was physico-chemically characterized, revealing prominent variations in functional groups, surface morphology, crystallinity, and surface area across samples subjected to individual and integrated pretreatment approaches. A high reducing sugar yield of 324.41 mg/g PL biomass was recovered after enzymatic hydrolysis of integrated UL-ChCl/glycerol pretreated PL samples under optimized conditions. The fermentation of the sugar hydrolysate yielded 121.36 mg/g ethanol with 89.61 % fermentation efficiency. Notably, DES recyclability experiments indicated significant performance up to the third cycle, after which activity marginally declined in correlation with sugar yield. The synergistic UL-ChCl/glycerol pretreatment process supports circular bioeconomy by promoting sustainable biomass conversion and offering a promising approach to reducing environmental impacts by utilizing agricultural waste for renewable energy production.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"404 ","pages":"Pages 83-93"},"PeriodicalIF":4.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143848250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhancing biomass enzymatic hydrolysis performance by modified DES lignin","authors":"Lan Yao ,&nbsp;Zhe Zhang ,&nbsp;Guangyu Chen ,&nbsp;Zhiyuan Sun ,&nbsp;Xiong Chen ,&nbsp;Haitao Yang","doi":"10.1016/j.jbiotec.2025.04.013","DOIUrl":"10.1016/j.jbiotec.2025.04.013","url":null,"abstract":"<div><div>The enzymatic hydrolysis of lignocellulose continues to be encumbered by elevated production costs and diminished cellulase efficiency. In this work, modified DES recovered lignin was obtained by grafting acrylamide and acryloyl chloride to enhance glucose release. At a cellulase dosage of 5 FPU/g-cellulose and pH of 5.5, modified lignin promoted glucose yield of dilute-acid-pretreated wheat straw by 158 % compared with control. The mechanism by which modified lignin promotes enzymatic hydrolysis was further explored. The binding constant was reduced from (3.3510 ± 0.8361)* 10⁴ to (2.7600 ± 0.6027)* 10³ L•mol⁻¹ after modification. Modified lignin could make α-helix content enhancement so that cellulase had a compact and stable spatial structure. Lignin binds within the catalytic tunnel of cellulase and that the modified lignin interacts with cellulase with increased hydrogen bonding, resulting in a more compact cellulase structure. The modified lignin might reduce the unproductive adsorption of cellulase, and increase stability and cellulose accessibility to reduce cellulase cost.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"403 ","pages":"Pages 115-125"},"PeriodicalIF":4.1,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143859398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}