Journal of biotechnology最新文献_第3页

Bioinformatics-assisted mining and design of novel pullulanase suitable for starch cold hydrolysis 生物信息学辅助下适用于淀粉冷水解的新型普鲁兰酶的挖掘与设计。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-01 DOI: 10.1016/j.jbiotec.2024.12.005

Xin Wang , Zixing Wang , Xueting Zhang , Yanli Zhang , Wenxia Zhang , Yu Zhang , Xuecheng Zhang , Yazhong Xiao , Yinliang Zhang , Wei Fang

{"title":"Bioinformatics-assisted mining and design of novel pullulanase suitable for starch cold hydrolysis","authors":"Xin Wang , Zixing Wang , Xueting Zhang , Yanli Zhang , Wenxia Zhang , Yu Zhang , Xuecheng Zhang , Yazhong Xiao , Yinliang Zhang , Wei Fang","doi":"10.1016/j.jbiotec.2024.12.005","DOIUrl":"10.1016/j.jbiotec.2024.12.005","url":null,"abstract":"<div><div>Cold-active pullulanases with good catalytic performance possess promising applications in cold hydrolysis of starch. Adopting bioinformatics-assisted mining strategies, 7 candidate cold-active pullulanases were initially screened out from IMG/MER database. Among the candidates, PulBs exhibited good thermostability and the highest specific activity of 147.4 U/mg. The half-life of PulBs was about 200 h at 35 °C. Employing PulBs as the initial enzyme, the active-site design of FuncLib was implemented to enhance the activity. The design PulBs-20 exhibited an enhanced specific activity of 209.9 U/mg, which was 1.4 times that of PulBs. Furthermore, the thermostability of PulBs-20 was augmented, with a half-life of 250 h at 35 °C. When applied in the cold hydrolysis of starch, PulBs-20 can effectively enhance the hydrolysis effect of raw starch. Supplemented with the raw starch-hydrolyzing α-amylase AmyZ1 and PulBs-20, the hydrolysis rate of raw corn starch increased to 53.5 %, which was 1.3 times that of using AmyZ1 alone. Due to its high hydrolysis activity and good thermostability, PulBs-20 can serve as an efficient accessory enzyme in starch cold hydrolysis.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"398 ","pages":"Pages 106-116"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142835780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to “Cell-free reduction of carboxylic acids with secreted carboxylic acid reductase” [J. Biotechnol. 382 (2024) 44–50] 用分泌的羧酸还原酶进行无细胞羧酸还原的勘误表[J]。生物工程学报，2016,44(2):444 - 451。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-01 DOI: 10.1016/j.jbiotec.2024.12.002

Dominic Goj , Stella Ebner , Melissa Horvat , Simon Arhar , Ludmila Martínková , Margit Winkler

引用次数: 0

Discovery and characterization of NADH oxidases for selective sustainable synthesis of 5-hydroxymethylfuran carboxylic acid 发现并鉴定用于选择性可持续合成 5- 羟甲基呋喃羧酸的 NADH 氧化酶。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-01 DOI: 10.1016/j.jbiotec.2024.11.009

Karishma Shah , Daniel Kracher , Peter Macheroux , Silvia Wallner , André Pick , Robert Kourist

{"title":"Discovery and characterization of NADH oxidases for selective sustainable synthesis of 5-hydroxymethylfuran carboxylic acid","authors":"Karishma Shah , Daniel Kracher , Peter Macheroux , Silvia Wallner , André Pick , Robert Kourist","doi":"10.1016/j.jbiotec.2024.11.009","DOIUrl":"10.1016/j.jbiotec.2024.11.009","url":null,"abstract":"<div><div>Efficient regeneration of NAD<sup>+</sup> remains a significant challenge for oxidative biotransformations. In order to identify enzymes with higher activity and stability, a panel of NADH oxidases (Nox) was investigated in the regeneration of nicotinamide cofactors for the oxidation of hydroxymethyl furfural (HMF) to 5-hydroxymethyl-2-furancarboxylic acid (HMFCA). We present novel Nox that exhibit remarkable catalytic activities, elevated thermal and pH stabilities, and higher intrinsic flavin loadings, thus eliminating the need for external flavin addition. The kinetic analysis of the NADH oxidases indicates that <em>Ad</em>Nox, <em>Gd</em>Nox, <em>Cm</em>Nox, and <em>Lv</em>Nox exhibit V<sub>max</sub> values of 86 U/mg, 50 U/mg, 4.3 U/mg, and 23 U/mg, respectively. When these NADH oxidases were applied in a HMF oxidation reaction, LvNox demonstrated the highest HMFCA yield of 97 % in the presence of 0.1 mM NAD and 10 mM HMF. In contrast to previously reported NADH oxidases from the same family, these NADH oxidases naturally accept NADPH as a substrate. Rapid kinetics experiments identified the oxidative reaction as the rate-limiting step of the reaction. NADH oxidases achieved high atom economy, a high reaction mass efficiency and a low process mass intensity. The findings contribute significantly to the field of biocatalysis and offer potential avenues for more environmentally friendly cofactor regeneration in chemical synthesis.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"398 ","pages":"Pages 18-28"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potency by design: Novel insights in transfection and purification for manufacturing of rAAV gene therapy vectors 效价设计：rAAV基因治疗载体转染和纯化的新见解。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-01 DOI: 10.1016/j.jbiotec.2024.12.007

Andreas Maccani , Robert Pachlinger , Peter Eisenhut, Sabine Unterthurner, Susanne Heider, Christoph Repik, Stefan Reuberger, Peter Andorfer, Johannes Lengler, Bernhard Kinastberger, Dieter Seczer, Petra Gruber, Alexander Apschner, Lucia Micutkova

{"title":"Potency by design: Novel insights in transfection and purification for manufacturing of rAAV gene therapy vectors","authors":"Andreas Maccani , Robert Pachlinger , Peter Eisenhut, Sabine Unterthurner, Susanne Heider, Christoph Repik, Stefan Reuberger, Peter Andorfer, Johannes Lengler, Bernhard Kinastberger, Dieter Seczer, Petra Gruber, Alexander Apschner, Lucia Micutkova","doi":"10.1016/j.jbiotec.2024.12.007","DOIUrl":"10.1016/j.jbiotec.2024.12.007","url":null,"abstract":"<div><div>This study investigates the crucial role of transfection methods in the manufacturability and potency of recombinant adeno-associated virus (rAAV) gene therapies. By employing a novel analytical approach, multiplex digital PCR (dPCR), we evaluated the impact of different transfection reagents and conditions on the scalability and quality of rAAV. Our research demonstrates that the selection of transfection approach significantly influences not only the yield and ease of scale-up but also the potency of the final product. Importantly, later changes to transfection parameters established in the early stages of development can be challenging, potentially compromising product quality and leading to comparability issues. Leveraging multiplex dPCR has proven instrumental in guiding these early-stage decisions, ensuring a reliable manufacturing process that consistently delivers high-quality therapeutic products. Our findings highlight the importance of optimizing transfection strategies early in development to guarantee the successful production of potent and cost-effective gene therapies, ultimately impacting patient accessibility.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"398 ","pages":"Pages 127-132"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142864376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Thermal degradation kinetics and purification of C-phycocyanin from thermophilic and mesophilic cyanobacteria 嗜热和中温蓝藻热降解动力学及c -藻蓝蛋白的纯化。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-01 DOI: 10.1016/j.jbiotec.2024.11.018

Supenya Chittapun , Kattiya Suwanmanee , Chatchol Kongsinkaew , Soisuda Pornpukdeewattana , Yusuf Chisti , Theppanya Charoenrat

{"title":"Thermal degradation kinetics and purification of C-phycocyanin from thermophilic and mesophilic cyanobacteria","authors":"Supenya Chittapun , Kattiya Suwanmanee , Chatchol Kongsinkaew , Soisuda Pornpukdeewattana , Yusuf Chisti , Theppanya Charoenrat","doi":"10.1016/j.jbiotec.2024.11.018","DOIUrl":"10.1016/j.jbiotec.2024.11.018","url":null,"abstract":"<div><div>The natural blue colorant C-phycocyanin (C-PC) has many potential applications but its poor heat stability limits its commercial use. This study compares the production and thermal stability of C-PC from two cyanobacteria: the thermophilic <em>Thermosynechococcus</em> sp. TUBT-T01 and the mesophilic <em>Synechococcus cedrorum</em> TISTR8589. <em>Thermosynechococcus</em> sp. produced nearly 1.9-fold more C-PC than <em>S. cedrorum</em>. Batch adsorption using a chromatographic cationic ion exchange resin (Streamline Direct HST1) was used to effectively purify the C-PC. The equilibrium adsorption capacity (<em>Q</em><sub><em>eq</em></sub>) of the resin for C-PC was the highest at pH 5. At this pH, the <em>Q</em><sub><em>eq</em></sub> for the thermophilic C-PC was 5.5 ± 0.1 mg mL⁻¹ , whereas for the mesophilic C-PC it was 1.5 ± 0.2 mg mL⁻¹ . Purification increased the concentration of the thermophilic C-PC by 5.9-fold, and that of mesophilic C-PC by 4.2-fold. The purity ratios of the final products from the two cyanobacteria were similar at ∼2.2. At 60 °C and pH 7, the C-PC of <em>Thermosynechococcus</em> sp. had ∼12-times longer half-life than the mesophilic C-PC; however, the productivity of the thermophilic C-PC was comparatively low because of a low biomass productivity of <em>Thermosynechococcus</em> sp.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"398 ","pages":"Pages 76-86"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769264","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Corrigendum to ‘Enhanced activation of signaling pathway by recombinant human adiponectin from genome-edited chickens’ [J. Biotechnol., Vol. 395 (2024) 95–99/0168–1656] 基因编辑鸡重组人脂联素增强信号通路激活的更正[J]。Biotechnol。， Vol. 395(2024) 95-99/0168-1656]。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-01 DOI: 10.1016/j.jbiotec.2024.11.019

Eunhui Yoo, Hee Jung Choi, Jae Yong Han

引用次数: 0

Alleviation of cadmium toxicity and minimizing its accumulation in rice plants by methyl jasmonate: Performance and mechanisms 茉莉酸甲酯减轻水稻镉毒性和减少其积累：性能和机制。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-01 DOI: 10.1016/j.jbiotec.2024.12.009

Ting Wei , Hong Li , Yuyao Wang , Ming Chi , Junkang Guo , Honglei Jia , Chao Zhang

{"title":"Alleviation of cadmium toxicity and minimizing its accumulation in rice plants by methyl jasmonate: Performance and mechanisms","authors":"Ting Wei , Hong Li , Yuyao Wang , Ming Chi , Junkang Guo , Honglei Jia , Chao Zhang","doi":"10.1016/j.jbiotec.2024.12.009","DOIUrl":"10.1016/j.jbiotec.2024.12.009","url":null,"abstract":"<div><div>Heavy metal pollution is a worldwide problem that threaten agricultural production and human health. Methyl jasmonate (MeJA) is a phytohormone that could enhance plant resistance against various stresses. However, the mechanism of MeJA in cadmium (Cd) uptake, distribution, and translocation in rice plants remains elusive. In this study, we found that the Cd induced-growth inhibition was ameliorated by MeJA. Upon MeJA application, Cd content in root and shoot was decreased by 10.15 % and 36.39 %, which paralleled with less Cd<sup>2 +</sup> influx of rice roots and depressed expression of the cation transporters (<em>OsNramp1</em> and <em>OsNramp5</em>). The subcellular distribution revealed that MeJA enriched Cd distribution in cell wall, which was accompanied by increased cell wall thickness and altered cell wall polysaccharide (pectin, cellulose, hemicellulose) content, meanwhile, the Cd content in pectin, cellulose, hemicellulose was increased, the FTIR analysis implied that functional groups (especially -OH and COO-) on cell wall were involved in Cd fixation. The root to shoot translocation of Cd was hindered by exogenous MeJA, this was validated by the decreased expression of <em>OsHMA2</em> in root and declined Cd level in xylem sap. Overall, our results revealed that MeJA could act as a foliar resistance control substance to reduce Cd accumulation in rice plants. The detailed molecular mechanisms of MeJA in Cd detoxification in plants still need further investigation.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"398 ","pages":"Pages 133-145"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142894664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Converting multiple hydrophobic aromatic plastic monomers into a single water-soluble substrate to increase bioavailability for the synthesis of polyhydroxyalkanoates by bacteria using batch, fed batch and continuous cultivation 将多种疏水芳香族塑料单体转化为单一水溶性底物，以提高细菌分批、补料分批和连续培养合成聚羟基烷酸酯的生物利用度。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-01 DOI: 10.1016/j.jbiotec.2024.12.008

Karthika Balusamy , Balaji V. Rokade , Manuel Bruch , Meg Walsh , William Casey , Federico Cerrone , Bryan Dalton , Patrick Jerome Guiry , Tanja Narančić , Kevin E. O’Connor

{"title":"Converting multiple hydrophobic aromatic plastic monomers into a single water-soluble substrate to increase bioavailability for the synthesis of polyhydroxyalkanoates by bacteria using batch, fed batch and continuous cultivation","authors":"Karthika Balusamy , Balaji V. Rokade , Manuel Bruch , Meg Walsh , William Casey , Federico Cerrone , Bryan Dalton , Patrick Jerome Guiry , Tanja Narančić , Kevin E. O’Connor","doi":"10.1016/j.jbiotec.2024.12.008","DOIUrl":"10.1016/j.jbiotec.2024.12.008","url":null,"abstract":"<div><div>We demonstrate the proof of concept of increasing the bioavailability of carbon substrates, derived from plastic waste, for their conversion to the biodegradable polymer polyhydroxyalkanoate [PHA] by bacteria and test various approaches to PHA accumulation through batch, fed batch and continuous culture. Styrene, ethylbenzene, and toluene are produced from the pyrolysis of mixed plastic waste (Kaminsky, 2021; Miandad et al., 2017), but they are volatile and poorly soluble in water making them difficult to work with in aqueous fermentation systems. By chemically converting these aromatic compounds to benzoic acid, and subsequently to its sodium salt, we increased the solubility and reduced the volatility of the substrate supplied to <em>Pseudomonas putida</em> CA-3 to accumulate polyhydroxyalkanoates. 1 L scale batch, fed batch, and continuous fermentations were carried out; the fed batch fermentation resulted in the maximum volumetric PHA productivity of 61.67 ± 7.34 mg L<sup>−1</sup> h<sup>−1</sup>; while batch and continuous, at a dilution rate, d = 0.2 h<sup>−1</sup>, fermentations resulted in 13.30 ± 0.01 and 4.06 ± 0.01 mg L<sup>−1</sup> h<sup>−1</sup> of PHA respectively.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"398 ","pages":"Pages 193-201"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142894667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Improved biosynthesis of tyrosol by epigenetic modification-based regulation and metabolic engineering in Saccharomyces cerevisiae 基于表观遗传修饰调控和代谢工程的酿酒酵母酪醇生物合成改进。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-01 DOI: 10.1016/j.jbiotec.2024.12.013

Guoli Wang , Xiqin Liang , Zhenke Wu, Bengui Fan, Jun Wang, Qiusheng Zheng, Defang Li, Tianyue An

{"title":"Improved biosynthesis of tyrosol by epigenetic modification-based regulation and metabolic engineering in Saccharomyces cerevisiae","authors":"Guoli Wang , Xiqin Liang , Zhenke Wu, Bengui Fan, Jun Wang, Qiusheng Zheng, Defang Li, Tianyue An","doi":"10.1016/j.jbiotec.2024.12.013","DOIUrl":"10.1016/j.jbiotec.2024.12.013","url":null,"abstract":"<div><div>Aromatic amino acids and their derivatives are high value chemicals widely used in food, pharmaceutical and feed industries. Current preparation methods for aromatic amino acid products are fraught with limitations. In this study, the efficient biosynthesis of aromatic amino acid compound tyrosol was investigated by epigenetic modification-based regulation and optimization of the biosynthetic pathway of aromatic amino acids. The production of tyrosol was significantly improved by the overexpression of m<sup>6</sup>A modification writer Ime4 and reader Pho92, and the positive regulator Gcr2. Introduction of Bbxfpk and deletion of Gpp1 further improved tyrosol production. Then the feedback inhibition of the shikimate pathway was relieved by the mutants Aro4<sup>K229L</sup> and Aro7<sup>G141S</sup>. The final tyrosol producing engineered strain was constructed by the deletion of <em>PHA2</em>, replacement of the native promoter of <em>ARO10</em> with the strong promoter PGK1p, and introduction of tyrosine decarboxylase PcAAS. In the background of m<sup>6</sup>A modification regulation, this strain ultimately produced 954.69 ± 43.72 mg/L of tyrosol, promoted by 61.7-fold in shake-flask fermentation.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"398 ","pages":"Pages 175-182"},"PeriodicalIF":4.1,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142921807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-yield soluble production of recombinant β-keratin from Gallus gallus feathers using an experimental design approach 利用实验设计方法从鸡羽中高效制备重组β-角蛋白。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2025-02-01 DOI: 10.1016/j.jbiotec.2025.01.002

Marcelo M. Ruas-Filho , Ana Maria Mazotto , Anderson S. Pinheiro

引用次数: 0