Journal of biotechnology最新文献_第4页

Markerless deletion of the putative type I and III restriction-modification systems in the cellulolytic bacterium Clostridium cellulovorans using a codBA-based counterselection technique 利用基于 codBA 的反选择技术，在纤维素溶解梭菌中无标记地删除推定的 I 型和 III 型限制性修饰系统。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-11-09 DOI: 10.1016/j.jbiotec.2024.11.001

Luciana Almeida, Aline Schöllkopf, Holger Edelmann, Armin Ehrenreich , Wolfgang Liebl

{"title":"Markerless deletion of the putative type I and III restriction-modification systems in the cellulolytic bacterium Clostridium cellulovorans using a codBA-based counterselection technique","authors":"Luciana Almeida, Aline Schöllkopf, Holger Edelmann, Armin Ehrenreich , Wolfgang Liebl","doi":"10.1016/j.jbiotec.2024.11.001","DOIUrl":"10.1016/j.jbiotec.2024.11.001","url":null,"abstract":"<div><div>Cellulose from lignocellulosic biomass (LB) is of increasing interest for the production of commodity chemicals. However, its use as substrate for fermentations is a challenge due to its structural complexity. In this context, the highly cellulolytic <em>Clostridium cellulovorans</em> has been considered an interesting microorganism for the breakdown of LB. <em>C. cellulovorans</em> does not naturally produce solvents in useful concentrations, but this could be achieved by metabolic engineering. Unfortunately, this is hampered by the lack of tools for genetic engineering. We describe a genetic system that allows strain engineering by the allelic-coupled exchange method. First, the Gram-positive origin of pUB110 was identified as a suitable clostridial 'pseudo-suicide' origin of replication for the construction of deletion vectors. Second, an efficient counterselection strategy based on a <em>codBA</em> cassette and the use of 5-fluorocytosine as the counterselective compound was employed. Third, since the prevention of DNA transfer by host restriction-modification (RM) systems is a critical barrier to genome engineering, deletion plasmids containing flanking regions for the putative type I (Clocel_1114) and III (Clocel_2651) RM systems were constructed and transferred into <em>C. cellulovorans</em>. The restriction-less strains <em>C. cellulovorans ΔClocel_1114</em> and <em>C. cellulovorans ΔClocel_2651</em> exhibit high conjugation efficiency and can be easily used for further metabolic engineering.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"397 ","pages":"Pages 22-31"},"PeriodicalIF":4.1,"publicationDate":"2024-11-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expanding the high-pH range of the sucrose synthase reaction by enzyme immobilization 通过酶固定化扩大蔗糖合成酶反应的高pH范围。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-11-08 DOI: 10.1016/j.jbiotec.2024.11.005

Hui Liu , Annika J.E. Borg , Bernd Nidetzky

{"title":"Expanding the high-pH range of the sucrose synthase reaction by enzyme immobilization","authors":"Hui Liu , Annika J.E. Borg , Bernd Nidetzky","doi":"10.1016/j.jbiotec.2024.11.005","DOIUrl":"10.1016/j.jbiotec.2024.11.005","url":null,"abstract":"<div><div>The glycosylation of an alcohol group from a sugar nucleotide substrate involves proton release, so the reaction is favored thermodynamically at high pH. Here, we explored expansion of the alkaline pH range of sucrose synthase (SuSy; EC 2.4.1.13) to facilitate enzymatic glycosylation from uridine 5’-diphosphate (UDP)-glucose. The apparent equilibrium constant of the SuSy reaction (UDP-glucose + fructose ↔ sucrose + UDP) at 30 °C increases by ∼4 orders of magnitude as the pH is raised from 5.5 to 9.0. However, the SuSy in solution loses ≥80 % of its maximum productivity at pH ∼7 when alkaline reaction conditions (pH 9.0) are used. We therefore immobilized the SuSy on nanocellulose-based biocomposite carriers (∼48 U/g carrier; ≥ 50 % effectiveness) and reveal in the carrier-bound enzyme a substantial broadening of the pH-productivity profile to high pH, with up to 80 % of maximum capacity retained at pH 9.5. Using reaction by the immobilized SuSy with automated pH control at pH ∼9.0, we demonstrate near-complete conversion (≥ 96 %) of UDP-glucose and fructose (each 100 mM) into sucrose, as expected from the equilibrium constant (<em>K</em><sub>eq</sub> = ∼7 × 10<sup>2</sup>) under these conditions. Collectively, our results support the idea of glycosyltransferase-catalyzed synthetic glycosylation from sugar nucleotide donor driven by high pH; and they showcase a marked adaptation to high pH of the operational activity of the soybean SuSy by immobilization.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 150-157"},"PeriodicalIF":4.1,"publicationDate":"2024-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficient biosynthesis of three rare formononetin derivatives by newly discovered Bacillus velezensis LQ5 新发现的 Velezensis LQ5 杆菌高效生物合成三种罕见的甲酮素衍生物。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-11-07 DOI: 10.1016/j.jbiotec.2024.11.003

Jiaqi Liu , Fan Yang , Wenxin Ji , Lin Zhao , Jing Han , Liangliang Chen , Fucheng Zhu , Jinao Duan , Sen Zhang

{"title":"Efficient biosynthesis of three rare formononetin derivatives by newly discovered Bacillus velezensis LQ5","authors":"Jiaqi Liu , Fan Yang , Wenxin Ji , Lin Zhao , Jing Han , Liangliang Chen , Fucheng Zhu , Jinao Duan , Sen Zhang","doi":"10.1016/j.jbiotec.2024.11.003","DOIUrl":"10.1016/j.jbiotec.2024.11.003","url":null,"abstract":"<div><div>Formononetin is a natural flavonoid existing widely in plants with many pharmacological effects. However, its application is limited by structure, poor water solubility and low bioavailability. In this study, <em>Bacillus velezensis</em> LQ5 was isolated from the inter-root soil of <em>Glycyrrhiza uralensis</em> Fisch for the first time and formononetin was firstly structurally modified by whole-cell catalysis of LQ5 to obtain formononetin-7-O-β-D-glucoside (FG), formononetin-7-O-β-(6''-O-succinyl)-D-glucoside (FGS) and formononetin-7-O-phosphate (FP). The selective preparation of the three products was achieved by adjusting the content of yeast extract, type and content of sugars, metal ions, pH and ATP content. The result confirmed that FP had the ideal drug-likeness properties and showed a greater ability to reduce intracellular reactive oxygen species levels and regulate oxidative enzymes. This work successfully established a biotransformation method for the efficient transformation of formononetin to produce high-value formononetin derivatives.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 116-126"},"PeriodicalIF":4.1,"publicationDate":"2024-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detrimental effects of UV-A radiation on antioxidant capacity and photosynthetic efficiency on a tropical microalga 紫外线辐射对热带微藻抗氧化能力和光合效率的有害影响

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-11-05 DOI: 10.1016/j.jbiotec.2024.10.013

Anna Isaia , Noémie Coulombier , Loïc Le Dean , Vincent Mériot , Thierry Jauffrais

{"title":"Detrimental effects of UV-A radiation on antioxidant capacity and photosynthetic efficiency on a tropical microalga","authors":"Anna Isaia , Noémie Coulombier , Loïc Le Dean , Vincent Mériot , Thierry Jauffrais","doi":"10.1016/j.jbiotec.2024.10.013","DOIUrl":"10.1016/j.jbiotec.2024.10.013","url":null,"abstract":"<div><div>Antioxidants are molecules able to neutralize reactive oxygen species with potential applications in the cosmetic or nutraceutical industries. Abiotic stressors, such as light intensity, ultraviolet (UV) radiation, or nutrient availability, can influence their production. In the perspective of optimizing and understanding the antioxidant capacity of microalgae, we investigated the effects of UV-A radiation on growth, and antioxidant and photosynthetic activities on <em>Tetraselmis</em>, a microalga genus known for its high antioxidant capacity. Cultures were exposed to UV-A radiation alongside to photosynthetically active radiation (PAR) in photobioreactors operated in continuous culture. UV-A exposure affects both the photosynthetic and antioxidant activities of <em>Tetraselmis</em>. Photosynthetic parameters suggest that UV-A has a negative effect on photosynthetic efficiency, particularly on the electron transport chain on short-term exposure (1–2 days). However, a resilience of most physiological parameters was observed over the experiment (10 days) suggesting a photochemical adaption over long-term exposure to UV-A radiation. Concerning the antioxidant capacity, UV-A exposure reduced the antioxidant capacity in <em>Tetraselmis</em> suggesting the use of antioxidant molecules to counteract reactive oxygen species production and prevent damage to photosystem II. Finally, the highest antioxidant capacity never observed with a <em>Tetraselmis</em> sp. was measured in cultures without UV addition, with an IC<sub>50</sub> of 2.87 ± 0.24 µg mL<sup>−1</sup>, a value close to the reference compounds Trolox and α-tocopherol. This study showed the great potential of <em>Tetraselmis</em> as a source of antioxidants under favorable culture condition and without UV-A radiations. Indeed, we discourage the use of UV-A to enhance antioxidant capacity in this species due to its negative impact on it and on the photosynthetic efficiency.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 104-115"},"PeriodicalIF":4.1,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142604648","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combinatorial metabolic engineering strategy of precursor pools for the yield improvement of spinosad in Saccharopolyspora spinosa 采用前体池组合代谢工程策略提高刺槐多糖的产量。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-11-02 DOI: 10.1016/j.jbiotec.2024.10.010

Li Cao , Xirong Liu , Danlu Yang , Ziyuan Xia , Zirui Dai , Lin Sun , Jing Fang , Zirong Zhu , Duo Jin , Jie Rang , Shengbiao Hu , Liqiu Xia

{"title":"Combinatorial metabolic engineering strategy of precursor pools for the yield improvement of spinosad in Saccharopolyspora spinosa","authors":"Li Cao , Xirong Liu , Danlu Yang , Ziyuan Xia , Zirui Dai , Lin Sun , Jing Fang , Zirong Zhu , Duo Jin , Jie Rang , Shengbiao Hu , Liqiu Xia","doi":"10.1016/j.jbiotec.2024.10.010","DOIUrl":"10.1016/j.jbiotec.2024.10.010","url":null,"abstract":"<div><div>Spinosad is an insecticide produced by <em>Saccharopolyspora spinosa</em>, and its larvicidal activity is considered a promising approach to combat crop pests. The aim of this study was to enhance the synthesis of spinosad through increasing the supply of acyl-CoAs precursor by the following steps. (i) Engineering the β-oxidation pathway by overexpressing key genes within the pathway to promote the synthesis of spinosad. The results showed that the overexpression of <em>fadD</em>, <em>fadE</em>, and <em>fadA</em>1 genes, as well as the co-expression of <em>fadA</em>1 and <em>fadE</em> genes, increased the yield of spinosad by 0.36-fold, 0.89-fold, 0.75-fold and 1.25-fold respectively. (ii) Employing combinatorial engineering of the β-oxidation pathway and ACC/PCC pathway to promote the synthesis of spinosad. The results showed that the co-expression of <em>fadE</em> and <em>pccA,</em> as well as <em>accC</em> and <em>fadE</em>, resulted in a 1.77-fold and 1.43-fold increase in spinosad production respectively. (iii) When exogenous triacylglycerol was added to the fermentation medium, the solely engineering of the β-oxidation pathway increased the yield of spinosad by 7.13-fold, reaching 427.23 mg/L. While the combinatorial engineering of both the β-oxidation pathway and ACC/PCC pathway increased the yield of spinosad by 9.61-fold, reaching 625.17 mg/L, and further optimization of the culture medium resulted in an even higher yield of spinosad, reaching 1293.43 mg/L. The results of this study indicate that the above combination strategy can promote the efficient biosynthesis of spinosad.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 127-139"},"PeriodicalIF":4.1,"publicationDate":"2024-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142568487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An overview of engineering microbial production of nicotinamide mononucleotide 烟酰胺腺嘌呤单核苷酸微生物工程生产概述。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-11-02 DOI: 10.1016/j.jbiotec.2024.10.014

Boting Li, Xiangfeng Meng, Weifeng Liu

引用次数: 0

Analysis of population heterogeneity in CHO cells by genome-wide DNA methylation analysis and by multi-modal single-cell sequencing 通过全基因组 DNA 甲基化分析和多模式单细胞测序分析 CHO 细胞中的群体异质性。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-10-31 DOI: 10.1016/j.jbiotec.2024.10.012

Elias Böhl , Günter Raddatz , Suki Roy , Lingzhi Huang , Jasrene Kaur Sandhu , Emeka Ignatius Igwe , Manuel Rodríguez-Paredes , Florian Böhl , Frank Lyko

{"title":"Analysis of population heterogeneity in CHO cells by genome-wide DNA methylation analysis and by multi-modal single-cell sequencing","authors":"Elias Böhl , Günter Raddatz , Suki Roy , Lingzhi Huang , Jasrene Kaur Sandhu , Emeka Ignatius Igwe , Manuel Rodríguez-Paredes , Florian Böhl , Frank Lyko","doi":"10.1016/j.jbiotec.2024.10.012","DOIUrl":"10.1016/j.jbiotec.2024.10.012","url":null,"abstract":"<div><div>CHO cells are major hosts for the industrial production of therapeutic proteins and their production stability is of considerable economic significance. It is widely known that CHO cells can rapidly acquire genetic alterations, which affects their genetic homogeneity over time. However, the role of non-genetic mechanisms, including epigenetic mechanisms such as DNA methylation, remains poorly understood. We have now used whole-genome bisulfite sequencing to establish single-base methylation maps of eight independent CHO cell lines. Our results identify CpG islands and low-methylated regions as conserved elements with dynamic DNA methylation. Interestingly, methylation patterns were found to cluster clearly along the three main branches of CHO evolution, with no directional changes over short culture periods. Furthermore, multi-ome single-cell sequencing of 9833 nuclei from three independent cultures revealed dynamic subpopulation structures characterized by robust expression differences in pathways related to protein production. Our findings thus provide novel insights into the epigenetic landscape and heterogeneity of CHO cells and support the development of epigenetic biomarkers that trace the emergence of subpopulations in CHO cultures.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 72-79"},"PeriodicalIF":4.1,"publicationDate":"2024-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142564514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor MfbHLH104 from Myrothamnus flabellifolia promotes drought tolerance of Arabidopsis thaliana by enhancing stability of the photosynthesis system 拟南芥转录因子MfbHLH104通过提高光合作用系统的稳定性促进拟南芥的耐旱性。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-10-29 DOI: 10.1016/j.jbiotec.2024.10.011

Zhuo Huang , Xiangying Xiang , Wenxin Xu , Li Song , Rong Tang , Duoer Chen , Qiao Li , Yujue Zhou , Cai-Zhong Jiang

{"title":"The transcription factor MfbHLH104 from Myrothamnus flabellifolia promotes drought tolerance of Arabidopsis thaliana by enhancing stability of the photosynthesis system","authors":"Zhuo Huang , Xiangying Xiang , Wenxin Xu , Li Song , Rong Tang , Duoer Chen , Qiao Li , Yujue Zhou , Cai-Zhong Jiang","doi":"10.1016/j.jbiotec.2024.10.011","DOIUrl":"10.1016/j.jbiotec.2024.10.011","url":null,"abstract":"<div><div>The resurrection plant <em>Myrothamnus flabellifolia</em> can survive extreme drought and desiccation conditions, and quickly recover after rewatering. However, little is known about the mechanism underlying the drought tolerance of <em>M. flabellifolia</em>. In this study, <em>MfbHLH104</em> was cloned and introduced into <em>Arabidopsis thaliana</em> due to the lack of a transgenic system for <em>M. flabellifolia.</em> MfbHLH104 is localized in the nucleus. Its N-terminal region has transactivation ability in yeast, and the C-terminal region may inhibit the transactivation ability. Overexpressing <em>MfbHLH104</em> significantly increased drought and salt tolerance of <em>A. thaliana</em> at both seedling and adult stages<em>.</em> It enhanced leaf water retention capacity by decreasing water loss rate and increasing drought- and abscisic acid (ABA) -induced stomatal closure. Additionally, it boosted osmolyte accumulation and ROS scavenging ability by up-regulating genes associated with osmolyte biosynthesis and antioxidant enzymes, and enhancing antioxidant enzyme activities. The expression of ABA-responsive genes were also promoted by MfbHLH104. Remarkably, RNA-seq analysis indicated that MfbHLH104 significantly up-regulated 32 genes (FDR < 0.05 and fold change ≥1.5) involved in photosynthesis related pathways (KEGG pathway No: ko00195, ko00196) under drought, which account for 18.7 % of the total up-regulated genes and the most enriched KEGG pathways. This result suggested that it may help to maintain the stability of the photosynthesis system under drought conditions.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 89-103"},"PeriodicalIF":4.1,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142557882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fractionation of lignin and fermentable sugars from wheat straw using an alkaline hydrogen peroxide/pentanol biphasic pretreatment 使用碱性过氧化氢/戊醇双相预处理法分馏小麦秸秆中的木质素和可发酵糖。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-10-18 DOI: 10.1016/j.jbiotec.2024.10.004

Yue Wu , Hui Zhang , Qixuan Lin , Ruonan Zhu , Lihong Zhao , Xingjie Wang , Junli Ren , Ling Meng

{"title":"Fractionation of lignin and fermentable sugars from wheat straw using an alkaline hydrogen peroxide/pentanol biphasic pretreatment","authors":"Yue Wu , Hui Zhang , Qixuan Lin , Ruonan Zhu , Lihong Zhao , Xingjie Wang , Junli Ren , Ling Meng","doi":"10.1016/j.jbiotec.2024.10.004","DOIUrl":"10.1016/j.jbiotec.2024.10.004","url":null,"abstract":"<div><div>To breakthrough the delignification saturation point (DSP) of alkaline hydrogen peroxide (AHP) pretreatment, a biphasic AHP/pentanol (AHPP) pretreatment was proposed in this work. The temperature and H<sub>2</sub>O<sub>2</sub> concentration were evaluated. Under the optimal conditions (110 °C, 2 h, 4 % H<sub>2</sub>O<sub>2</sub>), 70.73 % of lignin was removed, which was increased by 11.65 % than the traditional AHP pretreatment, indicating successful overcoming of the DSP by adding pentanol. 85.74 % and 88.62 % of glucan and xylan digestibility were achieved, respectively, which increased by 7.41 % and 5.87 % as compared to AHP pretreatment. Furthermore, the lignin extracted from the organic phase accounted for 38.51 % of the delignification, and it had a low molecular weight, effectively preserving the β-O-4 bonds. Finally, satisfied pentanol recovery (77.91 %) and delignification (57.19 %) along with excellent glucan (76.11 %) and xylan (77.52 %) digestibility were reached after fourth recycling of AHPP pretreatment. Therefore, AHPP pretreatment was a promising method for biomass valorization within biorefinery concept.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 62-71"},"PeriodicalIF":4.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid analysis of human primary dermal fibroblasts and epidermal keratinocytes after near-infrared exposure using mass spectrometry imaging 利用质谱成像技术分析近红外照射后人原生真皮成纤维细胞和表皮角质细胞的脂质。

IF 4.1 2区生物学

Journal of biotechnology Pub Date : 2024-10-18 DOI: 10.1016/j.jbiotec.2024.10.009

Laura van der Vloet , Zoé Ducarne , Ron M.A. Heeren , Anne C. Berends , Michiel Vandenbosch

{"title":"Lipid analysis of human primary dermal fibroblasts and epidermal keratinocytes after near-infrared exposure using mass spectrometry imaging","authors":"Laura van der Vloet , Zoé Ducarne , Ron M.A. Heeren , Anne C. Berends , Michiel Vandenbosch","doi":"10.1016/j.jbiotec.2024.10.009","DOIUrl":"10.1016/j.jbiotec.2024.10.009","url":null,"abstract":"<div><div>Photobiomodulation (PBM) therapy is the application of near-infrared (NIR) exposure to injuries or lesions to (among others) improve wound healing, reduce inflammation, and decreases acute and chronic pain. However, the understanding of the molecular mechanism of PBM, more specifically the effects of NIR on skin cells is still lacking behind. Lipids are essential components of cellular membranes that are integral to skin structure and function. This study aims to elucidate the impact of NIR exposure on the skin’s lipidome by investigating the molecular effect of NIR exposure on single skin cells. Primary human dermal fibroblasts (NHDFa) and human epidermal keratinocytes (HEKa) were exposed to NIR (850 nm) with a dose of 6.5 J/cm<sup>2</sup> for 5 consecutive days between 09.00 and 12.00 am. A workflow utilizing matrix-assisted laser desorption/ionization mass spectrometry imaging combined with liquid chromatography tandem mass spectrometry for lipidomics analysis was performed. This study provides evidence that adequate exposure of NIR influences lipid metabolism in NHDFa, whereas no alterations were found in HEKa. This work lays the groundwork in explaining the beneficial properties on both skin-related effects and systemic health benefits as seen in clinical studies.</div></div>","PeriodicalId":15153,"journal":{"name":"Journal of biotechnology","volume":"396 ","pages":"Pages 53-61"},"PeriodicalIF":4.1,"publicationDate":"2024-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142466229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0