红花多酚真菌发酵提取工艺优化及其对细胞代谢的影响。

IF 3.9 2区 生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Lesly Katleya Usme-Duque , Jesús A. Claudio-Rizo , José Alberto Nuncio-Esquivel , María I. León-Campos , Marisol Cruz-Requena , Leopoldo J. Ríos-González , Juan A. Ascacio- Valdés , Miguel A. Medina-Morales
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引用次数: 0

摘要

本研究旨在通过黑曲霉固态发酵,最大限度地挖掘花楸作为酚类化合物来源的潜力,重点评估提取的化合物的抗氧化活性及其对调节动物、癌症和植物细胞代谢的影响。首先,对浸渍提取物和发酵提取物的植物化学特征进行了比较。然后使用Plackett-Burman设计评估不同的培养条件(包括盐浓度、接种量、湿度和pH),以确定最重要的因素。然后用响应面法优化可水解酚类化合物的浓度。水分和KH₂PO₄浓度是提高酚含量的关键参数,最终浓度为43.440mg GAE/g。利用红外光谱和x射线衍射分析了这些提取物的化学成分,证实了其特征多酚官能团的存在,以及MgO、SiO₂和CaO等无机化合物的存在。对暴露于提取物的动物和植物细胞的体外代谢评估显示,发酵提取物显著刺激了3T3成纤维细胞和骨细胞的代谢。此外,提取物中的酚类化合物在48h时对HeLa和结肠癌细胞表现出细胞毒性作用。对于来自红色和绿色番茄、哈密瓜和西瓜种子的植物细胞,发酵提取物在暴露48小时后显著刺激了代谢活性。这些发现表明,黑曲霉发酵的苜蓿提取物具有广泛的生物技术应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Optimization of fungal fermentation for the extraction of polyphenols from Flourensia cernua and its effect on cellular metabolism
This study aimed to maximize the potential of Flourensia cernua as a source of phenolic compounds through solid-state fermentation with Aspergillus niger, focusing on evaluating the antioxidant activity of the extracted compounds and their effects on modulating the metabolism of animal, cancerous, and plant cells. Initially, a comparison of the phytochemical profiles between macerated and fermented extracts was conducted. Different culture conditions were then assessed using a Plackett-Burman design (including salt concentration, inoculum concentration, moisture, and pH) to identify the most significant factors. This was followed by a response surface methodology to optimize the concentration of hydrolyzable phenolic compounds. Moisture and KH₂PO₄ concentration were identified as critical parameters for enhancing phenolic content, resulting in a final concentration of 43.440 mg GAE/g. The chemical composition of these extracts was analyzed using infrared spectroscopy and X-ray diffraction, confirming the presence of characteristic polyphenol functional groups, along with inorganic compounds such as MgO, SiO₂, and CaO. In vitro metabolic evaluations of animal and plant cells exposed to the extracts revealed a marked stimulation of 3T3 fibroblast and bone cell metabolism with the fermented extract. Moreover, the phenolic compounds in the extract exhibited cytotoxic effects on HeLa and colon cancer cells at 48 hours. Regarding plant cells derived from red and green tomato, cantaloupe, and watermelon seeds, the fermented extract significantly stimulated metabolic activity after 48 hours of exposure. These findings suggest that fermented extracts of Flourensia cernua with Aspergillus niger hold promise for various biotechnological applications.
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来源期刊
Journal of biotechnology
Journal of biotechnology 工程技术-生物工程与应用微生物
CiteScore
8.90
自引率
2.40%
发文量
190
审稿时长
45 days
期刊介绍: The Journal of Biotechnology has an open access mirror journal, the Journal of Biotechnology: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. The Journal provides a medium for the rapid publication of both full-length articles and short communications on novel and innovative aspects of biotechnology. The Journal will accept papers ranging from genetic or molecular biological positions to those covering biochemical, chemical or bioprocess engineering aspects as well as computer application of new software concepts, provided that in each case the material is directly relevant to biotechnological systems. Papers presenting information of a multidisciplinary nature that would not be suitable for publication in a journal devoted to a single discipline, are particularly welcome.
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