Journal of Applied Research on Medicinal and Aromatic Plants最新文献

{"title":"Assessment of combining ability for targeted traits in periwinkle (Catharanthus roseus (L.) G. Don) through diallel analysis","authors":"Bolagam Ravikumar,&nbsp;K.K. Dhatt","doi":"10.1016/j.jarmap.2024.100539","DOIUrl":"https://doi.org/10.1016/j.jarmap.2024.100539","url":null,"abstract":"<div><p><em>Catharanthus roseus</em> (L.) G. Don is a vital summer season perennial semi shrub and multipurpose drought resilient flower crop of the tropical region of the Indian subcontinent. This industrially dominant crop is primarily availed as border, bedding and pot culture in landscaping; and also cultivated for its anti-cancer alkaloid compounds enriched plant parts. Hence, the present study was focused to explore diversity. Six inbred lines of periwinkle were crossed in full-diallel fashion with an objective of identifying superior cross-combinations for high general, specific and reciprocal combining ability. The analysis of variance for combining ability was highly significant for growth and flowering traits indicated existence of both additive and non-additive gene actions. However some traits showed only additive effect due to non-significant general combining ability. The parental lines of Vi-13–2 and Vi-15–2 expressed superior general combining effects for flower yield and considered as good general combiners for crossing programmes. The cross combinations Vi-15–1 × Vi-14–3 and Vi-16 × Vi-15–2 exhibited significantly positive specific combining ability effects for flower yield and considered as good specific direct combiners. High specific direct cross combiners necessitate poor × good, good × good and poor × poor parental combinations and can be contribute additive × dominance, additive × additive, dominance × dominance and epistatic gene interactions. The specific reciprocal cross combiners identified as Vi-14–3 × Vi-29 and Vi-14–3 × Vi-13–2 with superior expression in the reciprocal magnitudes for various traits might be due to cytoplasmic effects. The current investigation is designed to identify the best performing inbred lines for ornamental utilization as well as lines that can be exploited as parents in subsequent crosses.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"40 ","pages":"Article 100539"},"PeriodicalIF":3.9,"publicationDate":"2024-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140187833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ionic liquid microwave-assisted hydrodistillation extraction of Angelica sinensis essential oil and its own anti-inflammatory and antioxidant activities","authors":"Taotao Li ,&nbsp;Huanxian Shi ,&nbsp;Wenfei Wang ,&nbsp;Jia Li ,&nbsp;Tiantian Tang ,&nbsp;Yujiao Wang ,&nbsp;Ding Liu ,&nbsp;Kai Yang ,&nbsp;Xuan Wang ,&nbsp;Jie Wang ,&nbsp;Ning Xia ,&nbsp;Jinhui Wang ,&nbsp;Chao Chen ,&nbsp;Xiaoxiao Ge ,&nbsp;Junbo Zou ,&nbsp;Dongyan Guo ,&nbsp;Yajun Shi ,&nbsp;Yundong Xie ,&nbsp;Zhenfeng Wu ,&nbsp;Ming Yang ,&nbsp;Xiaofei Zhang","doi":"10.1016/j.jarmap.2024.100538","DOIUrl":"10.1016/j.jarmap.2024.100538","url":null,"abstract":"<div><p>In this study, ionic liquid microwave-assisted hydrodistillation (IL-MAHD) was employed to overcome the challenges of substantial emulsification and low extraction yield which occur in the extraction process of <em>Angelica sinensis</em> essential oil (AEO). Additionally, an online detection system was introduced into the AEO extractor to enable real-time monitoring during the extraction process. Optimal conditions were determined as follows: material-to-liquid of 1:10, ionic liquid concentration of 0.5%, microwave power of 400 W, extraction time of 4 h, condensation temperature of 20 °C, and cooling temperature of 40 °C. Under these optimized conditions, the AEO yield reached 0.7707%, marking a 1.4066-fold increase compared to microwave-assisted hydrodistillation (MAHD) and a 3.7615-fold increase compared to hydrodistillation (HD). Meanwhile, IL-MAHD exhibited the highest relative content of (<em>Z</em>)-ligustilide (73.424%), and the corresponding AEO displayed significant anti-inflammatory and antioxidant effects at concentrations of 7.8125, 15.625, and 31.25 µg/g. The results of extraction kinetics and online monitoring suggested that the IL-MAHD achieved the fastest extraction yield of AEO. This study provides a new perspective on exploring a potential cost-efficient technology for the industrial extraction of essential oils.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"39 ","pages":"Article 100538"},"PeriodicalIF":3.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140126003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive assessment of the extraction of Phyllanthus emblica L. based on the G1-Entropy Method and Response Surface Methodology","authors":"Zhen-Yu Gai,&nbsp;Ying Wang,&nbsp;Ming-Hua Xian,&nbsp;Dan Tang ,&nbsp;Shu-Mei Wang","doi":"10.1016/j.jarmap.2024.100536","DOIUrl":"10.1016/j.jarmap.2024.100536","url":null,"abstract":"<div><p>In this study, the G1-Entropy method was employed together with a response surface methodology (RSM) approach to establish a multi-index-based system for evaluating and optimizing the extraction of bioactive compounds from <em>Phyllanthus emblica</em> L. Seven chemical classes' contents were evaluated, including TPC (total phenolic content), TC (tannin content), DEY (dry extract yield), GA (gallic acid), CO (corilagin), CA (chebulagic acid), and EA (ellagic acid). The resultant extracts were also assessed for their antioxidant activity, as measured based on their DPPH radical, ABTS⁺, and hydroxyl radical scavenging activity levels and their capacity to reduce iron ions. The inhibition of fluorescent AGEs generation was also assessed in BSA-Fru and MGO-BSA model assays to gauge the anti-glycation activity of these extracts. In total, a comprehensive score was derived based on 13 indices and corresponding weight values, with these scores then being used to determine the extraction conditions that yielded the best outcome. Through this strategy, the ideal extraction processing parameters were found to include an ethanol concentration of 56%, a liquid-solid ratio of 8 mL/g, and an extraction duration of 100 min. Using these optimized conditions yielded a predicted comprehensive score (Y) of 94.91, while the response value based on three independent variables from the validation experiments performed using these conditions was 92.03. These results highlight the promise of leveraging <em>Phyllanthus emblica</em> as a resource for use in the cosmetic, food, biochemical, and pharmaceutical industries.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"39 ","pages":"Article 100536"},"PeriodicalIF":3.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140047353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification and characterization of novel resistant genotypes of Gymnema sylvestre (Retz.) R. Br. ex Sm. against invasive mealybug species, Phenacoccus solenopsis Tinsley and Paracoccus marginatus Williams and Granara de Willink for sustainable pest management","authors":"Kadanakuppe Thammayya Shivakumara ,&nbsp;Akula Chinapolaiah ,&nbsp;Manikyanahalli Chandrashekara Keerthi ,&nbsp;Ramesan Syamala Ramya ,&nbsp;Bheemanna Somanna Gotyal","doi":"10.1016/j.jarmap.2024.100534","DOIUrl":"10.1016/j.jarmap.2024.100534","url":null,"abstract":"<div><p>Madhunashini, <em>Gymnema sylvestre</em> R. Br. ex Sm. (F: Asclepiadaceae) is an important industrial medicinal plant that has various pharmacological properties like antidiabetic, antiobesity, anticarcinogenic and neuroprotective. The invasive mealybugs, <em>i.e., Phenacoccus solenopsis</em> and <em>Paracoccus marginatus,</em> are causing significant yield losses to <em>G. sylvestre</em> in India. The identity of the mealybug species, <em>P. solenopsis</em> and <em>P. marginatus,</em> were confirmed both morphologically and molecularly by using <em>Cytochrome c oxidase subunit I</em> (<em>COI</em>) gene with (GenBank Accession No. OQ349395 and OQ358124). The pest incidence occurs throughout the year, but its peak activity was observed during the summer months (April to August). In the current investigation, 30 genotypes were examined for their resistance against mealybugs in their natural field environment for three consecutive years from 2019 to 2021 in the geographical region of Anand, Gujarat, India. The pooled analysis showed that the severity of infestation ranged between 0.41 and 3.57. Our study results revealed that 10% of the genotypes <em>i.e.</em> DGS-18, DGS-26, and DGS-28, were highly resistant. Furthermore, 16.66% of the genotypes, <em>i.e.</em> DGS-13, DGS-15, DGS-22, DGS-30, and DGS-33 were categorized as resistant. The novel resistant genotypes identified in the present study will be helpful for the management of <em>P. solenopsis</em> and <em>P. marginatus,</em> in <em>G. sylvestre</em>. The resistant genotypes have excellent scope for reducing the use of pesticide usage as well as help in mitigating pesticide residue.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"39 ","pages":"Article 100534"},"PeriodicalIF":3.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140047531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Variability in essential oil content and constituent profile of damask rose (Rosa damascena Mill.) at altered intervals of harvest in the Indian Western Himalaya","authors":"Shalika Rathore ,&nbsp;Kanchan Kundlas ,&nbsp;Rakesh Kumar","doi":"10.1016/j.jarmap.2024.100537","DOIUrl":"10.1016/j.jarmap.2024.100537","url":null,"abstract":"<div><p>The cultivation of essential oil-bearing damask rose (<em>Rosa damascena</em> Mill.) is majorly focused on enhancing the essential oil content and quality. The current study aims to investigate the variability in the essential oil level and component makeup of damask rose at different harvests at regular intervals during the flowering season. The study consists of two vegetation years i.e. 2021 and 2022 with five harvesting dates, <em>viz</em>., April 6, April 13, April 20, April 27, and May 4 during both years. The damask rose variety ‘Jwala’ was used for the experiment and plants taken for flower harvest were 6 and 7 years old during 2021 and 2022, respectively. The essential oil content of damask rose fresh flowers was significantly higher in the first vegetation year (0.044%) and April 20 (0.048%) harvest and decreased with delay in harvesting after that. GC-MS profiling displayed citronellol and geraniol as major contributors to essential oil area percentage but quantitative variations were observed with variability in temperature and precipitation. The highest percentage of citronellol was recorded on the May 4 harvest. The highest geraniol content was recorded from April 27 than other dates of harvests but remained statistically analogous with April 13 and April 20. Essential oil decreased at both delayed and early harvests. Throughout the crop harvest season, harvesting on April 20 harvest date produced the highest essential oil content compared to early and late flush harvest. This suggests that the middle of the flowering season is suitable for procuring the highest essential oil yield under the mid-hill conditions of the western Himalayan region. These findings could have significant economic implications for the market demand and pricing for damask rose essential oil.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"39 ","pages":"Article 100537"},"PeriodicalIF":3.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140047182","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of thermal processing on bioactive compounds contents and antioxidant capacities of unripe and ripe Solanum anguivi Lam. fruit accessions","authors":"Aisha Musaazi Sebunya Nakitto ,&nbsp;Yusuf B. Byaruhanga ,&nbsp;Anika E. Wagner ,&nbsp;John H. Muyonga","doi":"10.1016/j.jarmap.2024.100535","DOIUrl":"https://doi.org/10.1016/j.jarmap.2024.100535","url":null,"abstract":"<div><p>The bioactive compounds contents (BCC), total antioxidant capacity (TAC), and thus the potential health-promoting capacity of fruits and vegetables may depend on their processing history. <em>Solanum anguivi</em> Lam. fruits (SALF) are consumed as vegetables and have been traditionally used to manage diabetes, possibly due to the possession of bioactive compounds, including phenolics and saponins. Data on the effect of thermal treatments versus ripeness stage and accession on the BCC and TAC of SALF is currently limited. The present study investigated the effect of drying temperature (35, 55, and 85 °C), and traditional cooking method (boiling and steaming) at 100 °C, and duration (15, 30, 45, 60, and 120 min) on the BCC and TAC of SALF. Spectrophotometry was used to analyse the TAC and BCC (total phenolic, flavonoid saponins, and vitamin C contents), and gravimetry for total alkaloids. The effect of drying on the BCC and TAC of SALF significantly varied with drying temperature, accession, ripeness stage, cooking method, and cooking duration. Drying at 35 °C increased the total phenolics and TAC of unripe SALF more than 2-fold, and retained the highest vitamin C and total flavonoid contents. The effect of drying on saponins and alkaloids was dependent on the accession and ripeness stage. The effect of cooking on the BCC and TAC was significantly influenced by the cooking method and duration. Boiling and steaming increased the total phenolics and TAC 4-fold and 3-fold, respectively. Cooking reduced the saponins and did not affect flavonoid contents. Exceptionally, 15 min of boiling did not affect the total saponins and increased the total flavonoids. Total alkaloids were decreased by steaming and increased by boiling. However, very long boiling and steaming time (120 min) greatly increased the alkaloids 2-fold and 1.5-fold, respectively. All the thermal treatments negatively affected vitamin C. Overall, the thermal treatment that resulted in the highest total phenolics and TAC of SALF was boiling (for 15 min), which may result in the highest health benefits of SALF upon consumption.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"39 ","pages":"Article 100535"},"PeriodicalIF":3.9,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140113640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Different approaches to improve the tolerance of aromatic and medicinal plants to salt stressed conditions","authors":"Nadia Lamsaadi ,&nbsp;Omar Farssi ,&nbsp;Ahmed El Moukhtari ,&nbsp;Mohamed Farissi","doi":"10.1016/j.jarmap.2024.100532","DOIUrl":"https://doi.org/10.1016/j.jarmap.2024.100532","url":null,"abstract":"<div><p>Salinity is a global environmental constraint that hinders the growth and development of various aromatic and medicinal plants (AMPs) and declines their yield and productivity. This is due mainly to the overaccumulation of salt ions in plant tissues, which affects negatively the physiology and cellular metabolic homeostasis. Recent investigations highlighted that the major functions driving plant growth and development and plant’s survival such as seed germination, photosynthesis and water and mineral nutrition, are severely reduced in various AMPs under salinity constraint. Regarding their economic potential and their importance in worldwide health, improving the growth and production of AMPs, especially under salt-stressed conditions, is an important necessity and represents a global challenge. In this regard, seed priming and application of exogenous compounds have been reported among the most applied ecofriendly techniques to mitigate the effects of salt stress in various AMPs. Here, we first analyzed the harmful effects of salt stress on the growth and production of AMPs, by reporting the effects of salt ions on seed germination, flowering, photosynthesis, primary and secondary metabolite biosynthesis and essential oil production. This review also describes the potential effects of seed priming and exogenous compounds application in enhancing salt stress tolerance of several AMPs. Results and proposal mechanisms by which these approaches mitigate the negative effects of salinity on AMPs’ growth and productivity are analyzed and critically discussed.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"39 ","pages":"Article 100532"},"PeriodicalIF":3.9,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139748958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Function and regulation of a chitinase gene during Panax notoginseng defense response to root rot","authors":"Hanlin Wang ,&nbsp;Jun Hai ,&nbsp;Yuan Qu ,&nbsp;Xiuming Cui ,&nbsp;Diqiu Liu ,&nbsp;Guanze Liu","doi":"10.1016/j.jarmap.2024.100533","DOIUrl":"https://doi.org/10.1016/j.jarmap.2024.100533","url":null,"abstract":"<div><p>Root rot severely affects <em>Panax notoginseng</em>, and an important causual pathogen is <em>Fusarium solani</em>. The pathogenesis-related proteins, including chitinase, are important for plant defenses against pathogens. In this study, a chitinase gene (<em>PnCHI3</em>) in <em>P. notoginseng</em> was isolated on the basis of published transcriptome data. Both the <em>F. solani</em> infection and exogenous methyl jasmonate treatment stimulated the expression of <em>PnCHI3</em>. In addition, the purified PnCHI3 recombinant protein had a significant inhibitory effect on root rot pathogens. Meanwhile, the resistance of transgenic tobacco over-expressing <em>PnCHI3</em> was strengthened. The <em>PnCHI3</em> promoter, which was cloned via genome walking, was employed to drive the <em>GUS</em> reporter gene expression in tobacco. Various signaling molecules, abiotic stresses, and fungal pathogens activated the <em>PnCHI3</em> promoter. The results of an electrophoretic mobility shift assay and a yeast one-hybrid experiment revealed that the <em>P. notoginseng</em> transcription factor WRKY4 bound to the <em>PnCHI3</em> promoter W-box cis-element and activated <em>PnCHI3</em> transcription. In summary, the hormone signals including jasmonic acid and WRKY transcription factor enhances the expression of root rot resistance-related gene <em>PnCHI3</em> in <em>P. notoginseng</em> in order to protect against invasion of <em>F. solani</em>.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"39 ","pages":"Article 100533"},"PeriodicalIF":3.9,"publicationDate":"2024-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139748957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity and population structure of different Schisandraceae accessions by EST-SSR markers","authors":"Guangli Shi ,&nbsp;Binhong Zhu ,&nbsp;Dan Sun ,&nbsp;Zhenxing Wang ,&nbsp;Jun Ai ,&nbsp;Jiaqi Geng ,&nbsp;Xiang Li ,&nbsp;Miao Yu ,&nbsp;Chengcheng Zhao ,&nbsp;Xiyu Zhang","doi":"10.1016/j.jarmap.2024.100531","DOIUrl":"10.1016/j.jarmap.2024.100531","url":null,"abstract":"<div><p><span><em>Schisandra chinensis</em></span> (<em>Schisandra</em>) is one of the most potent and traded herbs in Asian countries that has been described as a tonic and sedative in the Chinese, Korean, and American pharmacopoeias. It was included in the International Pharmacopoeia (edited by WHO) and the European Pharmacopoeia. Due to its enormous socioeconomic worth, there is a growing demand for <em>S. chinensis</em><span><span> cultivation; however, relatively little was known about the genetic diversity of the </span>Schisandraceae plants. In this study, we assessed the genetic diversity and population structure of the Schisandraceae using 122 accessions collected from different regions of China. Using 38 expressed sequence tag-simple sequence repeats (EST-SSR) markers, a total of 78 polymorphic bands were detected in these accessions. The primers had an average polymorphism information content (PIC) of 0.407 (from 0.096 to 0.831), indicating a moderate level of polymorphism that might support the analysis of genetic diversity in Schisandraceae plants. Cluster analysis was carried out based on the results of EST-SSR amplification. Accessions with a genetic similarity coefficient of 0.61 were divided into two clusters: cluster I comprising 4 </span><em>Schisandra sphenanthera</em> accessions and 106 <em>S. chinensis</em> accessions, and cluster II containing 12 <em>Kadsura coccinea</em><span> accessions. The PCoA plot obtained was in accordance with the results of NJ cluster analysis. Finally, all accessions could be effectively identified. Based on population structure analysis, 122 samples were divided into five subgroups. The Fst, and Nm of the five subgroups were 0.044 and 5.376, respectively, indicating that 95.6% of the genetic variation was within subgroups and just 4.4% was between subgroups. This may be closely related to the strong gene flow between subgroups, the relative concentration of the collected regions, and the breeding method<span><span> of monoecious plants. Overall, our findings will facilitate the Schisandraceae research by offering genetic background data to support breeding improvement and </span>germplasm resource conservation.</span></span></p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"39 ","pages":"Article 100531"},"PeriodicalIF":3.9,"publicationDate":"2024-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139551537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Applying DNA barcoding to identify the cultivated provenance of Fritillaria taipaiensis P. Y. Li and its related species","authors":"Yuze Liang ,&nbsp;Jie Zhang ,&nbsp;Xue Wang ,&nbsp;Tiantian Gao ,&nbsp;Haifeng Li ,&nbsp;Dequan Zhang","doi":"10.1016/j.jarmap.2024.100530","DOIUrl":"10.1016/j.jarmap.2024.100530","url":null,"abstract":"<div><p><span><em>Fritillaria</em><em> taipaiensis</em></span><span> P. Y. Li is one of the original species of Fritillariae Cirrhosae Bulbus (FCB), namely “Chuanbeimu”. It is difficult to identify the cultivated provenance of the FCB based on morphology, because of complexity of its original species. Therefore, it is necessary to develop an efficient molecular method to discriminate these species. In the present study, nine plastid genomes from cultivated </span><em>F. taipaiensis</em><span> and its related species were newly obtained using Next-generation sequencing, in addition to ITS sequences<span> from Sanger sequencing. Then, DNA super, universal, and specific barcodes were compared in identifying these cultivation provenances using P-distance, Tree-building, and Online-blast methods. For the Tree-building, all individuals of </span></span><em>F. taipaiensis</em> could be successfully identified and DNA super barcode showed the highest success rate (91.67%) and reliability. Moreover, the super barcode could discriminate 88.88% of the individuals of these species except that of <em>F. sichuanica</em> using the Online-blast method in the NCBI database; however, only two individuals of <em>F. taipaiensis</em> were successfully identified using ITS sequences. This study further demonstrated that DNA super barcode based on plastid genome possesses a perfect ability of species discrimination on cultivation provenance of FCB.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"39 ","pages":"Article 100530"},"PeriodicalIF":3.9,"publicationDate":"2024-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139496082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}