{"title":"Assessment of the quality of plant material in commercial products of ground ivy herb (Glechomae hederaceae herba)","authors":"Karolina Grabowska, Weronika Ziółkowska-Żygas, Agnieszka Galanty, Dagmara Wróbel-Biedrawa, Irma Podolak","doi":"10.1016/j.jarmap.2025.100634","DOIUrl":"10.1016/j.jarmap.2025.100634","url":null,"abstract":"<div><div><em>G. hederacea</em> has a well-established position in traditional medicinal systems of Europe and Asia. Despite the significant increase in the number of products based on this plant on the European market, there is as yet no European Pharmacopoeia monograph on <em>Glechomae herba,</em> therefore legally binding quality standards of medical-grade ground ivy herb in member states that ratified Convention on the elaboration of a European Pharmacopoeia. This study aimed to investigate differences in the quality of the commercially available single-herb loose <em>G. hederacea</em> products in the aspects of authentication, purity of plant material and content of bioactive substances. As the therapeutic potential of <em>G. hederacea</em> is related to the presence of phenolic compounds, the study on qualitative and quantitative analysis of phenolics by HPLC method was performed. The analysis revealed significant differences in the quality of ground ivy plant material, in terms of the content of bioactive compounds. The determined levels of rosmarinic acid, chlorogenic acid, gallic acid and rutin in the products ranged from 0.10 to 4.55 mg/g DW; 0.18–1.57 mg/g DW; 0.02–1.28 mg/g DW; to 6.01 mg/g DW, respectively. Additionally, the products varied significantly in the sum of determined polyphenols (1.5 – 7.16 mg/g DW). The findings highlight the need to establish the standards and requirements for bioactive phenolic content to ensure high-quality plant material with significant medicinal value. Additionally, our study suggests that rosmarinic acid, rutin, chlorogenic acid, and the total polyphenol content could serve as quality markers for <em>Glechomae hederaceae herba</em> in pro-health applications.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100634"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhilong He , Zhao Yang , Cui Dong , Shanshan Yan , Jing Lin , Hexigeduleng Bao , Fan Tang , Leilei Zhang , Cheng Zhu , Yan Hu , Yanfei Ding
{"title":"Identification of Dendrobium officinale species and authentication of commercial products by DNA barcoding and high-resolution melting analysis","authors":"Zhilong He , Zhao Yang , Cui Dong , Shanshan Yan , Jing Lin , Hexigeduleng Bao , Fan Tang , Leilei Zhang , Cheng Zhu , Yan Hu , Yanfei Ding","doi":"10.1016/j.jarmap.2025.100633","DOIUrl":"10.1016/j.jarmap.2025.100633","url":null,"abstract":"<div><div><em>Dendrobium officinale</em> is widely used as a precious traditional medicinal plant and health food in China. However, because of its high nutritional and medicinal value, it is often targeted for adulteration or mislabeling. This work aims to exploit DNA barcoding combined with high-resolution melting analysis to differentiate <em>Dendrobium officinale</em><strong>(</strong><em>D. officinale</em>)species from non-<em>D. officinale</em> species and to further identify its botanical origin. The internal transcribed spacer 2 (ITS2) was targeted as a candidate barcode for <em>Dendrobium</em> species identification. High resolution melting(HRM)method allowed differentiating the six <em>Dendrobium</em> species in three clusters with 95 % confidence interval, which is consistent with the sequencing analysis. ITS2 sequencing results implied that 43 % of commercial <em>Dendrobium</em> products may be mislabeled. Thus, ITS2 DNA barcoding with HRM assay could be used as a simple, specific and cost-effective tool to identify different <em>Dendrobium</em> species and commercial <em>Dendrobium</em> products, and to authenticate the origin of <em>D. officinale.</em></div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100633"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Xihui Bian , Yuxia Liu , Jiaqi Xie , Chunyan Zhao , Xiaoyao Tan , Weilu Tian
{"title":"Accurate and intelligent quantification of adulterated Angelicae Sinensis Radix by a novel ensemble method with near-infrared spectroscopy","authors":"Xihui Bian , Yuxia Liu , Jiaqi Xie , Chunyan Zhao , Xiaoyao Tan , Weilu Tian","doi":"10.1016/j.jarmap.2025.100640","DOIUrl":"10.1016/j.jarmap.2025.100640","url":null,"abstract":"<div><div>The integrity and authenticity of Chinese medicinal materials are essential for ensuring the efficacy of medicines and safety of patients. <em>Angelicae Sinensis Radix</em> (ASR), a highly valued traditional Chinese medicine (TCM), is often substituted or mixed with lower-cost alternatives. In this study, a novel method was proposed for the quantification of adulterated ASR using near-infrared (NIR) spectroscopy combined with chemometrics. Binary and ternary adulterated ASR samples were prepared by mixing ASR with <em>Angelicae Pubescentis Radix</em> (APR) and <em>Chuanxiong Rhizoma</em> (CR), and their NIR spectra were measured in the range of 1000–1800 nm. Sample subsets were initially selected using Monte Carlo (MC) sampling, followed by the application of whale optimization algorithm (WOA) to select variables and establish an extreme learning machine (ELM) model, referred to as MC-WOA-ELM. The iteration number and percentage of training subsets for MC-WOA-ELM were optimized to further improve the predictive performance of model. The predictive ability of the proposed method was compared with partial least squares (PLS), ELM and WOA-ELM for both binary and ternary adulterated ASR samples. The results indicate that MC-WOA-ELM achieves the highest prediction accuracy. Therefore, the proposed method is rapid and intelligent for the quantification of adulterated ASR samples.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100640"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143935039","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Heavy metal pollution in Iranian medicinal plants, a review of sources, distribution, and health implications","authors":"Farzaneh Mirzabayati, Amir Hossein Hamidian","doi":"10.1016/j.jarmap.2025.100637","DOIUrl":"10.1016/j.jarmap.2025.100637","url":null,"abstract":"<div><div>Environmental pollution, particularly with heavy metals, is a critical issue in assessing the quality of medicinal plants. The concentration of heavy metals in these plants depends on factors such as growth environment, species, and processing methods. Contaminants absorbed by plants can enter the human body through consumption. This review examines the impact of heavy metals—cadmium (Cd), lead (Pb), arsenic (As), copper (Cu), chromium (Cr), zinc (Zn), and nickel (Ni)—on medicinal plants in Iran. It explores how environmental contamination affects plant quality and analyzes trends in measurement techniques and metal concentrations over time. Results show that Cd has the most significant negative impact on plant growth (63 %), followed by Pb (21 %), Cr (11 %), and Zn (5 %). Cd exceeded WHO limits in 47 % of samples, while Pb and Zn exceeded limits in 26 % and 20 %, respectively. Cd and Pb also significantly reduced germination rates. Contamination with Cd and Pb poses serious health risks, including kidney damage, bone disorders, cancer, neurological issues, and reproductive harm. Strict quality control, sustainable farming, and public awareness are essential to mitigate these risks. This study highlights the need for further research, particularly in industrial regions like Tehran, Khuzestan, Isfahan, and Fars, to ensure the safety of medicinal plants.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100637"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Zhong-hao Zhang , Zhong-hua Zhao , Tian-yi Cao , Cai-rong Yang , Zi-zhong Tang , Shi-ling Feng , Shu Yuan , Sheng-lin Hu , Ming Yuan
{"title":"Comparative analysis of three extraction methods for ginger leaf polysaccharide: Chemical composition, antioxidant activity, and hypolipidemic effects","authors":"Zhong-hao Zhang , Zhong-hua Zhao , Tian-yi Cao , Cai-rong Yang , Zi-zhong Tang , Shi-ling Feng , Shu Yuan , Sheng-lin Hu , Ming Yuan","doi":"10.1016/j.jarmap.2025.100639","DOIUrl":"10.1016/j.jarmap.2025.100639","url":null,"abstract":"<div><div>Ginger leaves are often discarded in agriculture, exacerbating continuous cropping obstacles. Polysaccharide, due to their low toxicity and diverse bioactivities, have garnered considerable interest. To isolate ginger leaf polysaccharide (GLP), we compared three extraction methods: hot water extraction, ultrasonic-assisted hot water extraction, and ultrasonic-assisted aqueous two-phase system extraction. Response surface methodology optimization revealed ultrasonic-assisted hot water extraction as the most efficient method, achieving a yield of 9.31 % under a liquid-solid ratio of 30 mL/g and an ultrasonic power of 150 W at 65 ℃ for 22 min. Polysaccharide from different extraction methods showed difference in monosaccharide composition and molecular weight. The ginger leaf polysaccharide demonstrated strong antioxidant <em>in vitro</em> and hypolipidemic activities in <em>Caenorhabditis elegans</em>, and that from the ultrasonic-assisted hot water method showed the highest bioactivity. These findings highlight ultrasonic-assisted hot water extraction as the optimal method for efficient extraction of GLP and discover GLP’s potential for pharmaceutical and functional food applications.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100639"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Does the exogenous application of brassinosteroids affect the photosynthetic, morphological characteristics, and THC concentrations of Cannabis sativa L. under drought stress?","authors":"Shiva Barooti , Mohsen Edalat , Mostafa Oveisi , Seyed Abdolreza Kazemeini , Ruhollah Naderi","doi":"10.1016/j.jarmap.2025.100635","DOIUrl":"10.1016/j.jarmap.2025.100635","url":null,"abstract":"<div><div>Drought stress represents a significant constraint on the productivity of crops globally. The application of plant growth regulators, particularly brassinosteroids (BRs), has demonstrated potential in enhancing plant tolerance to drought conditions. This study aimed to examine the effects of exogenous BR application on the photosynthetic and morphological characteristics, as well as the concentrations of Delta-9-Tetrahydrocannabinol (THC), in <em>Cannabis sativa</em> subjected to drought stress. A split-plot experimental design was implemented over two growing seasons, with the primary factor being the planting date (May 20, June 5, June 20) and the secondary factor being the irrigation level (80–100 %, 50–60 %, and 30–40 % of field capacity). Exogenous BR treatments (0, 200, and 400 μg/L) were applied as the tertiary factor. The results indicated that drought stress significantly diminished photosynthetic efficiency, plant growth, and THC concentrations in <em>C. sativa</em>. Conversely, the exogenous application of BRs alleviated the adverse effects of drought, leading to enhanced photosynthetic performance, improved morphological traits, and increased THC levels compared to drought-stressed plants that did not receive BR treatment. These findings suggest that the exogenous application of BRs may serve as a promising strategy to improve drought tolerance and the medicinal properties of <em>C. sativa</em>. The study offers valuable insights into the role of plant growth regulators in modulating the physiological and biochemical responses of this important medicinal plant under conditions of water limitation.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100635"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
G. Mahendiran , Y.N. Venkatesh , Anna Jose , B. Manu , M. Ashajyothi , K. Sreedevi , S.N. Sushil
{"title":"Morpho-molecular identification of Barioscapus cordiae (Marshall) (Coleoptera: Curculionidae): A serious pest of Cordia myxa L.","authors":"G. Mahendiran , Y.N. Venkatesh , Anna Jose , B. Manu , M. Ashajyothi , K. Sreedevi , S.N. Sushil","doi":"10.1016/j.jarmap.2025.100638","DOIUrl":"10.1016/j.jarmap.2025.100638","url":null,"abstract":"<div><div>The lasoda weevil, <em>Barioscapus cordiae</em> (Marshall) is redescribed with digital images of habitus, morphometric details and images of diagnostic characters. The monotypic weevil genus <em>Barioscapus</em> Pajni and Kohli is easily recognised from other genera in the tribe by the presence of very closely related proxcoxae, antennae inserted at two-third length of rostrum from base, scape remote from eyes, prosternum without a depression before coxae, and exposed pygidium. The first DNA barcode was obtained and reported for molecular identification using cytochrome oxidase I (COI). The estimated total percentage of damage to fruits and seeds caused by <em>B</em>. <em>cordiae</em> to <em>Cordia myxa</em> was 82.07–88.08 %.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100638"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143918453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Yuchen Tang , Wennan Nie , Yao Zhang , Cunhao Li , Zhu Wei , Haiyang Chen , Yunfei Hu , Wenlong Li
{"title":"Rapid identification of Fritillaria spp. using multi-wavebands spectroscopy and multi-source data fusion strategies","authors":"Yuchen Tang , Wennan Nie , Yao Zhang , Cunhao Li , Zhu Wei , Haiyang Chen , Yunfei Hu , Wenlong Li","doi":"10.1016/j.jarmap.2025.100636","DOIUrl":"10.1016/j.jarmap.2025.100636","url":null,"abstract":"<div><div><em>Fritillaria</em> spp., a kind of typical medicine food homology plant, is in growing demand for rapid identification due to its diverse types. This study aims to utilize the mid-infrared (MIR) and ultraviolet-visible-near infrared (UV–VIS–NIR) spectroscopy for the first time to identify <em>Fritillaria</em> spp<em>.</em> Two machine learning techniques of random forest (RF) and support vector machine (SVM) are employed to establish classification models based on spectral data from various <em>Fritillaria</em> spp<em>.</em> Data fusion strategies including low-level, mid-level and high-level fusions are applied to combinate MIR and UV–VIS–NIR data. The result indicates that data fusion improved the classification of <em>Fritillaria</em> spp. compared to using individual spectral data. The RF classifier showed superior performance over the SVM model. The high-level data fusion model achieved the highest prediction accuracy of 93.31 %. This study demonstrates that integrating MIR and UV–VIS–NIR spectroscopy with data fusion techniques offers a feasible, non-destructive, and rapid approach for classifying <em>Fritillaria</em> spp., highlighting the potential for the identifying related medicinal food homology products.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100636"},"PeriodicalIF":3.8,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143927545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Li Gao , Ruiwen Yang , Liqin Li , Bing Han , Guoyin Kai
{"title":"Application of gas chromatography-ion mobility spectrometry in traditional Chinese medicines: A review","authors":"Li Gao , Ruiwen Yang , Liqin Li , Bing Han , Guoyin Kai","doi":"10.1016/j.jarmap.2025.100632","DOIUrl":"10.1016/j.jarmap.2025.100632","url":null,"abstract":"<div><div>Gas chromatography-ion mobility spectrometry (GC-IMS) has emerged as a powerful analytical technique, renowned for its rapid analysis and operational simplicity, particularly in the detection of trace compounds. While its applications span various scientific domains, its potential in the field of traditional Chinese medicine (TCM) remains relatively underexplored. This review systematically examines the current applications and methodological parameters of GC-IMS in TCM research, with particular emphasis on its transformative potential in quality assessment and authentication processes. The versatility of GC-IMS has been demonstrated across multiple facets of TCM analysis, including (1) differentiation of various Pao Zhi processing techniques and their impact on TCM quality, (2) authentication of TCM specimens through parameters such as storage duration and geographical origin, (3) establishment of robust quality evaluation criteria for TCM formulations, (4) detection and profiling of volatile organic compounds (VOCs) across different growth stages, (5) taxonomic classification of TCM species, and (6) comprehensive characterization of TCM constituents. The integration of GC-IMS in TCM research offers substantial promise for advancing quality control. Its rapid analytical capabilities, coupled with high sensitivity, position it as an indispensable tool for TCM authentication and quality evaluation, with potential applications throughout various stages of TCM production and distribution.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100632"},"PeriodicalIF":3.8,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143637181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"An integrative analysis of population genetics and species distribution modeling of Gymnema sylvestre in India","authors":"Kanchana Vaishnav , Aakash Maurya , Anjala Durgapal , T.S. Rana","doi":"10.1016/j.jarmap.2025.100631","DOIUrl":"10.1016/j.jarmap.2025.100631","url":null,"abstract":"<div><div><em>Gymnema sylvestre</em> (Retz.) R. Br. ex Schult., is a potent anti-diabetic, tropical plant species from Africa to Australia. It has been used since centuries both in indigenous and modern medicine systems to treat diabetes, snake bite, malaria, jaundice, asthma, bronchitis, cardiopathy, leukoderma, eye problems, obesity, etc. In India, the species is significantly threatened by unsustainable harvesting practices for pharmaceutical industries and habitat destruction. Moreover, the continuing climate change may affect its distributional range. Hence, the objective of this study was to evaluate the genetic health (genetic diversity and population genetic structure) and potential geographical range of <em>G. sylvestre</em> under both current (1970–2000) and projected future climatic conditions (2050 and 2070 under Representative Concentration Pathway 4.5 and RCP8.5) to develop effective conservation, management, and sustainable utilization approaches. A moderate level of genetic diversity was found {Observed Heterozygosity (H<sub>o</sub> = 0.391), Nei’s Expected Heterozygosity (H<sub>e</sub> = 0.382), and Shannon’s information index (I = 0.656)}, with majority of the genetic variation accumulated within populations (74 %), and very high Nei’s genetic differentiation among populations (F<sub>ST</sub> = 0.281) was noticed as the Gene flow among populations (N<sub>m</sub> = 0.637) was medium. Various genetic clustering methods yielded two distinct (K = 2) genetic clusters in 118 <em>G. sylvestre</em> individuals proportionate to the geographical isolation. Compared to the present, all the future projections came pessimistically with a reduction in the high and moderate suitable areas. However, 2070 would favor the species in adaptation to the changed climate with increased suitable area compared to the year 2050.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"46 ","pages":"Article 100631"},"PeriodicalIF":3.8,"publicationDate":"2025-02-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632044","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}