Journal of Applied Research on Medicinal and Aromatic Plants最新文献

{"title":"Process optimization, kinetics, and thermodynamics for the selenium extraction from Cardamine violifolia","authors":"Qi Zhang ,&nbsp;Shengliang Zhu ,&nbsp;Shengrong Zhang ,&nbsp;Zhanye Xv ,&nbsp;Fang Jin ,&nbsp;Shengdong Zhu","doi":"10.1016/j.jarmap.2025.100671","DOIUrl":"10.1016/j.jarmap.2025.100671","url":null,"abstract":"<div><div>Selenium supplementation is an effective means for its deficient population to maintain their health. The selenium extraction is one of the most important procedures for preparing its supplements. This work provided a systematic investigation on the selenium extraction process from <em>Cardamine violifolia.</em> Firstly, the selenium extraction process was optimized by using the single factor analysis and response surface optimization. Its optimal conditions were particle size 60 mesh, stirrer rotation speed 125 r min⁻¹, initial extraction pH 9, extraction temperature 70 ℃, ratio of water to material 100 ml g⁻¹ and extraction time 27 min. Under its optimal conditions, the selenium concentration in the extracted liquid reached 4.45 µg ml⁻¹. Then its kinetics were investigated, and its kinetic data was in good agreement with the pseudo-first order kinetic model. Its mass transfer coefficient <em><strong>k</strong></em> ranged from 0.0765 to 0.1411 min⁻¹ when its temperature changed from 40 to 70 ℃. And its apparent activation energy <strong>ΔE</strong> was 22.84 kJ mol⁻¹. Finally, its thermodynamic parameters were calculated, its enthalpy change <strong>ΔH</strong> was 30.07 KJ mol⁻¹, its entropy change <strong>ΔS</strong> was 99.3 J mol⁻¹ K⁻¹, and its Gibbs free energy <strong>ΔG</strong> was range from −1.00 to −3.96 KJ mol⁻¹ when its temperature changed from 40 to 70 ℃. These thermodynamic parameters indicated that the selenium extraction process from <em>C. violifolia</em> was a spontaneous, endothermic and entropy increasing process. This work provides useful information to industrialize the selenium extraction from <em>C. violifolia</em> for its supplement preparation.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"49 ","pages":"Article 100671"},"PeriodicalIF":3.6,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145324991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-assisted NaDES extraction of phenolics from Butea monosperma flowers: Optimization and cosmetic applications","authors":"Kullapon Kesonkan ,&nbsp;Liudmila Yarovaya ,&nbsp;Anupong Joompang ,&nbsp;Panatorn Puwanatanaboon ,&nbsp;Nicha Kanpakdee ,&nbsp;Chanikan Sonklin ,&nbsp;Supachai Jadsadajerm ,&nbsp;Prasat Kittakoop ,&nbsp;Awat Wisetsai","doi":"10.1016/j.jarmap.2025.100672","DOIUrl":"10.1016/j.jarmap.2025.100672","url":null,"abstract":"<div><div>In alignment with green chemistry principles, this study presents an eco-friendly method for extracting bioactive compounds from <em>Butea monosperma</em> flowers using natural deep eutectic solvents (NaDES). Lactic acid (LA) was combined with various hydrogen bond acceptors, sodium acetate (SA), glucose (Glu), and fructose (Fru) to prepare NaDES. Ultrasound-assisted extraction (UAE) was employed as a green and efficient extraction technique. Extraction efficiency was optimized based on total phenolic content (TPC) and antioxidant activity, evaluated using both DPPH and ABTS assays. Optimal conditions were achieved using LA:SA (3:1, 20 % water) for 45 min (<strong>LS-1</strong>) and LA:Glu (9:1, 40 % water) (<strong>LG-1</strong>) for 60 min, yielding high TPC values (1069.49 ± 12.39 and 1064.93 ± 15.33 mg GAE/L) with strong DPPH scavenging activity (0.089 ± 0.005 and 0.055 ± 0.005 %v/v). ABTS assay further confirmed such antioxidant activity of these extracts. The crude extracts were characterized using UV and NMR spectroscopy. Two major flavonoids, isobutrin (<strong>1</strong>) and butrin (<strong>2</strong>), were successfully isolated and structurally confirmed using UV, IR, and NMR techniques. Their antioxidant activities were also confirmed by the DPPH and ABTS assays. Finally, the most potent extract, prepared using <strong>LS-1</strong>, was successfully incorporated into a water-based cosmetic formulation, demonstrating its potential for sustainable cosmetic applications.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"49 ","pages":"Article 100672"},"PeriodicalIF":3.6,"publicationDate":"2025-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145324990","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"St. John's wort root-associated beneficial bacteria with vermicompost augment specialized metabolites without penalizing biomass yield by improving photosynthesis and soil microbial properties","authors":"Yog Raj ,&nbsp;Nilofer Ali ,&nbsp;Aparna Maitra Pati ,&nbsp;Rakshak Kumar ,&nbsp;Rakesh Kumar","doi":"10.1016/j.jarmap.2025.100670","DOIUrl":"10.1016/j.jarmap.2025.100670","url":null,"abstract":"<div><div>Reintroducing beneficial rhizosphere microbes associated with wild medicinal plants back into the rhizosphere during cultivation is expected to increase the efficacy of herbal medicine. However, the effectiveness of this approach for St. John’s wort (<em>Hypericum perforatum</em> L.) has not been explored, nor has it been evaluated alongside other plant biostimulants, such as seaweed extract and non-host-specific plant-beneficial rhizobacteria, that may also impact plant growth and specialized metabolites. To fill these knowledge gaps, in present study a two-year factorial randomized block design experiment was conducted using vermicompost amendment and plant biostimulants, derived from microbial origin (consortia of host plant-associated and non-host-specific plant-beneficial rhizobacteria) and macroalgae-based (seaweed extract). Dry biomass yield of main medicinal material (<em>Hyperici herba</em>) significantly (<em>P = 0.05</em>) influenced by the interaction effect; while contents of specialized metabolites by individual treatments. Seaweed extract significantly maximized the contents of specialized metabolites, particularly pseudohypericin and hypericin; however, it substantially reduced the biomass yield. In both year, biomass yield was not significantly (<em>P &gt; 0.05</em>) influenced by the individual treatment of non-host-specific plant-beneficial rhizobacteria; however, in combination with vermicompost amendment at 5 Mg ha⁻¹ the biomass yield numerically increased compared to the seaweed extract treatment. Nevertheless, specialized metabolites were minimally increased by non-host-specific plant-beneficial rhizobacteria. Unlike seaweed extract and non-host-specific plant-beneficial rhizobacteria, host plant-associated rhizobacteria simultaneously enhanced specialized metabolites, especially hyperforin, and the biomass yield either alone or in combination. Across both years, the maximum biomass yield was achieved under the combined application of host plant-associated plant-beneficial rhizobacteria and vermicompost amendment at 5 Mg ha⁻¹. This treatment also improved net photosynthetic rate, internal water use efficiency, soil microbial biomass carbon, and respiration compared to control and rest of the treatments. Thus, the integrating host plant-associated plant-beneficial rhizobacteria with vermicompost amendment offers a sustainable approach to increase the efficacy of St. John’s wort herbal medicine without compromising the biomass yield.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"49 ","pages":"Article 100670"},"PeriodicalIF":3.6,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145324989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colchicine induces polyploidization in sweet marjoram (Origanum majorana)","authors":"Emmanuel Nkosinathi Kunene ,&nbsp;Jian-Zhi Huang ,&nbsp;Jong-Yi Fang","doi":"10.1016/j.jarmap.2025.100669","DOIUrl":"10.1016/j.jarmap.2025.100669","url":null,"abstract":"<div><div>Sweet marjoram (<em>Origanum majorana</em> L<em>.</em>) is an aromatic plant widely used in traditional medicine, food, cosmetics, and pharmaceutical industries. However, unfavorable climatic conditions resulting from global warming negatively affect its productivity. This challenge may be addressed by developing more tolerant cultivars. To support this, the present study evaluated the efficacy of colchicine for inducing polyploidy in sweet marjoram, with the aim of facilitating cultivar improvement. Seeds were treated with colchicine at concentrations of 0.000 %, 0.025 %, 0.050 %, 0.100 %, 0.200 %, 0.400 %, and 0.800 % for 24 h. Treated seeds were germinated <em>in vitro</em>, and surviving seedlings were established under greenhouse conditions. The ploidy status of all greenhouse plants was initially screened by stomatal assay and subsequently confirmed by flow cytometry. Results showed that seed germination percentages (30 %-46 %) did not differ significantly among treatments; however, seedling survival declined as colchicine concentration increased, with no seedlings surviving at 0.400 % and 0.800 %. Seventeen putative polyploids were identified, of which fourteen were confirmed as true polyploids and three as mixoploids. The fourteen true polyploids included two triploids, ten tetraploids, and two pentaploids. The efficiencies of polyploid induction were 11.1 % at 0.025 %, 40 % at 0.050 %, 75 % at 0.100 %, and 80 % at 0.200 % colchicine. Compared with diploids, polyploid plants exhibited wider leaves, larger stomata, and reduced stomatal density. The true polyploid plants represent valuable genetic resources for breeding new sweet marjoram cultivars.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"49 ","pages":"Article 100669"},"PeriodicalIF":3.6,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145324988","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green extraction of bioactive compounds from orange peel waste using NADES and microwave-assisted technique: A CatBoost-GMDH ensemble optimized by mantis search algorithm","authors":"Mostafa Khajeh ,&nbsp;Mansour Ghaffari-Moghaddam ,&nbsp;Jamshid Piri ,&nbsp;Afsaneh Barkhordar ,&nbsp;Didem Saloglu","doi":"10.1016/j.jarmap.2025.100668","DOIUrl":"10.1016/j.jarmap.2025.100668","url":null,"abstract":"<div><div>The increasing need for sustainable and efficient extraction methods has led to interest in green technologies for the extraction of bioactive compounds from agricultural waste. Orange peel, a rich of polyphenols and flavonoids, offers significant potential for sustainable application. This research formulated a novel multi-task optimization approach combining the Mantis Search Algorithm (MSA) with hybrid machine learning models to extract bioactive compounds from orange peel waste using natural deep eutectic solvents (NADES) and microwave-assisted extraction (MAE). The holistic strategy employed a multi-task optimization approach that concurrently optimized four key components: feature selection, model hyperparameters, ensemble weights, and process parameters. The process parameters investigated included microwave power (302–495 W), extraction temperature (31–59 °C), extraction time (5.2–30 min), and mass-to-solvent ratio (41–80 mg/mL). Three machine learning models were developed and systematically compared: CatBoost, Group Method of Data Handling (GMDH), and their weighted ensemble fusion. The ensemble MSA-hybrid model exhibited the best predictive performance with R² of 0.656, 0.981, and 0.990 for total phenolic content, total flavonoid content, and DPPH radical scavenging activity, respectively. Temperature was found to be the most significant process parameter for all response variables, followed by extraction time and mass-to-solvent ratio. The multi-task optimization approach successfully developed robust predictive models capable of guiding extraction parameter selection for improved bioactive compound yields. Extensive validation using thorough residual analysis, stability testing, and confidence interval analysis reaffirmed model reliability and generalizability. This novel study was successful in offering valuable industry-ready solutions for sustainable bioactive compound extraction while supporting agricultural waste valorization and circular economy concepts.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"49 ","pages":"Article 100668"},"PeriodicalIF":3.6,"publicationDate":"2025-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145270310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal changes in chemical content of edible flowers of common daisy (Bellis perennis L.)","authors":"Lucia Nedorost Ragasová,&nbsp;Jarmila Neugebauerová,&nbsp;Tomáš Kopta,&nbsp;Robert Pokluda","doi":"10.1016/j.jarmap.2025.100667","DOIUrl":"10.1016/j.jarmap.2025.100667","url":null,"abstract":"<div><div>In the context of increasing popularity of alternative sources of food such as edible flowers the chemical composition of edible flowers of <em>Bellis perennis</em> L. was evaluated in this study. Common daisy<em>,</em> widely spread across the world, contains many bioactive compounds including ones with strong antioxidant activity, such as L-ascorbic acid or polyphenols. <em>B. perennis</em> flowers were sampled monthly during 2016–2023 have been analysed for chemical composition. Ascorbic acid content (AA) was monitored for eight years, and the rest of chemical assays including total phenolic content (TPC), total flavonoid content (TFC), total antioxidant capacity (TAC) was determined for 3 years. Out of 8-year monitoring of L-ascorbic acid content, significantly highest content was detected in February and March (768.5 ± 36 mg/kg FW in March 2017; 673.6 ± 18.9 mg/kg FW in Feb. 2017; 429.6 ± 1.6 mg/kg FW in Feb. 2022; 414.4 ± 11.2 mg/kg FW in Feb. 2019 and 412.2 ± 2.4 mg/kg FW in March 2018). The small mostly irregular fluctuations in TFC, TPC and TAC were determined, however for phenolic and flavonoid content the gradual increase from February till June was observed. Total antioxidant capacity in <em>B. perennis</em> ranged from 1.7 to 3.6 g TE/100 g DW. Despite these monthly fluctuations in bioactive compound content, the widespread availability of a common daisy throughout the year makes it a promising and accessible source of health-beneficial nutrients.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"49 ","pages":"Article 100667"},"PeriodicalIF":3.6,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145222399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of phenotypic diversity in pods and seeds traits of natural populations of Gleditsia sinensis in Guizhou Province, China","authors":"Jun Luo ,&nbsp;Xiaoyong Dai ,&nbsp;Bing Yang ,&nbsp;Jin Chen ,&nbsp;Shuang He","doi":"10.1016/j.jarmap.2025.100666","DOIUrl":"10.1016/j.jarmap.2025.100666","url":null,"abstract":"<div><div>In order to reveal the phenotypic variation degree and variation pattern of the natural population of Gleditsia sinensis in Guizhou Province, and lay a theoretical foundation for the genetic improvement, germplasm identification and variety breeding of <em>G. sinensis</em>. This study measured 12 phenotypic traits of seeds and pods in 180 individual trees from 17 natural <em>G. sinensis</em> populations in Guizhou Province and detected rich phenotypic diversity. The results suggest that the phenotypic variation of <em>G. sinensis</em> seeds and pods in Guizhou is extremely rich, with intrapopulation variation being the primary source of variation. The average phenotypic differentiation coefficient is 23.87 %, and the average coefficient of variation is 11.59 %. The variation in phenotypic traits of <em>G. sinensis</em> seeds and pods is geographically discontinuous. Generally, pods show north-south variation along the same longitude, while seeds exhibit east-west variation along the same latitude. Inter-population variability in pods (14.67 %) is higher than in seeds (8.96 %), suggesting greater variability in pod traits and higher stability in seed traits. This multi-level variation provides a scientific basis for the innovative utilization of high-quality genetic resources of <em>G. sinensis</em>. Principal component analysis shows that the first three principal components (size of pods, size and weight of seeds, weight of pods) account for 81.9 % of the cumulative contribution, essentially reflecting most of the information on phenotypic traits of <em>G. sinensis</em>. A comprehensive evaluation of the natural populations of <em>G. sinensis</em> based on 12 pods and seeds traits finds that the CeHeng (CH) population has the best overall performance, followed by WangMo (WM), DeJiang (DJ), and Weng'an (WA) populations. Suitable breeding materials can be selected from these four populations based on breeding objectives. This research findings lay a crucial foundation for advancing the genetic improvement of <em>G. sinensis</em> and provide a scientific underpinning for the development of breeding strategies and management practices.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"49 ","pages":"Article 100666"},"PeriodicalIF":3.6,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145222418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent progress in DNA-based approaches for authenticating herbal materials and botanical dietary supplements","authors":"Rong Chen ,&nbsp;Wenjun Song ,&nbsp;Hongling Chen ,&nbsp;Tongyi Peng ,&nbsp;Huilin Li ,&nbsp;Sheng Ding","doi":"10.1016/j.jarmap.2025.100663","DOIUrl":"10.1016/j.jarmap.2025.100663","url":null,"abstract":"<div><h3>Background</h3><div>Herbal materials and botanical supplements are widely consumed for health benefits, yet concerns persist due to prevalent adulteration, highlighting the need for reliable authentication methods.</div></div><div><h3>Scope and approach</h3><div>This review examines DNA identification techniques for quality control of both botanical dietary supplements and their constituent raw materials. The methods relevant to this field are split into two categories: mainstream analytical and emerging detection techniques. The focus is on their applicability for detecting raw and multi-ingredient processed products, quantification potential, and on-site testing suitability.</div></div><div><h3>Key findings and conclusions</h3><div>DNA identification methods, such as PCR variants like TaqMan and high-resolution melting (HRM), as well as DNA barcoding, have been effectively applied by enterprises and regulatory bodies for authenticating herbal materials, addressing many quality control challenges. Emerging technologies, including LAMP, RPA and digital PCR, combined with tools like microfluidics, lateral flow assays, and CRISPR/Cas systems, hold great potential for rapid, on-site testing. However, practical challenges such as cost, accessibility, and stability remain significant barriers, requiring further research to enable their widespread application in real-world scenarios.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"49 ","pages":"Article 100663"},"PeriodicalIF":3.6,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145222402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of saffron (Crocus sativus L.) adulteration using 1H NMR spectroscopy","authors":"Shivashish Gulabrao Sonone,&nbsp;Dattatraya Dinkar Gore,&nbsp;Inder Pal Singh","doi":"10.1016/j.jarmap.2025.100665","DOIUrl":"10.1016/j.jarmap.2025.100665","url":null,"abstract":"<div><div><em>Crocus sativus</em> L. (saffron) is the most valuable spice worldwide, yet its limited production and high market value make it highly susceptible to adulteration with plant substitutes and synthetic dyes. In this study, ¹H Nuclear Magnetic Resonance (NMR) spectroscopy was applied as a rapid, non-destructive technique for saffron authentication and detection of eight plant adulterants and one synthetic dye in a single experiment. Methanolic and hexane extracts were prepared to capture a broad polarity range of metabolites from authentic saffron, known adulterants, and commercial brands. Spectral comparison targeted key saffron markers, including picrocrocin, crocin, and crocetin. Most brand samples closely matched the authentic reference, with minor variations that can attributed to seasonal and geographical factors, whereas the ZK sample lacked diagnostic saffron peaks and instead displayed resonances consistent with Sudan IV and <em>Arnica montana</em>, confirming adulteration. Quantitative NMR (qNMR) was further employed for picrocrocin determination, and the method was validated in terms of selectivity, specificity, linearity (R² &gt; 0.998), precision (intra- and inter-day RSD &lt; 5.5 %), robustness (consistent results across varied scan numbers and relaxation delays). The method demonstrated a limit of detection (LOD) of 0.443 µg/mL and a limit of quantification (LOQ) of 1.342 µg/mL for picrocrocin, indicating high sensitivity and suitability for reliable quantification. Overall, these findings establish ¹H NMR spectroscopy as a robust qualitative analytical platform for both authentication and detection of adulteration of saffron. Its integration into routine quality control and regulatory frameworks could strengthen consumer protection and ensure the integrity of high-value herbal and food products.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"49 ","pages":"Article 100665"},"PeriodicalIF":3.6,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145222401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound extraction, quantification, and antioxidant activity of flavonoids from Aquilaria sinensis leaves","authors":"Junhao Deng ,&nbsp;Ning Tang ,&nbsp;Li Xu ,&nbsp;Feiyue Chen ,&nbsp;Xiang Zhou ,&nbsp;Weizhuo Tang ,&nbsp;Xiongzhuo Tang","doi":"10.1016/j.jarmap.2025.100664","DOIUrl":"10.1016/j.jarmap.2025.100664","url":null,"abstract":"<div><div>In this work, the ultrasound-assisted extraction (UAE) method was performed to obtain the total flavonoids (TF) from <em>Aquilaria sinensis</em> (<em>A. sinensis</em>) leaves, followed by optimization of the extraction factors by using a single-factor experiments combined with Box–Behnken response surface methodology to enhance the TF yield. Subsequently, the composition of the two representative flavonoids, mangiferin and genkwanin, were quantitatively determined by high-performance liquid chromatography (HPLC). Finally, the antioxidant activity of the TF was evaluated using an H₂O₂-induced oxidative stress model in intestinal porcine epithelial cells-jejunum (IPEC-J2) cells. The results demonstrated that under the optimized UAE conditions (70 % of ethanol concentration, liquid-to-material ratio at 40:1, 45 min of ultrasonic time, and 400 w of ultrasonic extraction power), the extraction rate of the TF reached 5.73 %, which was significantly higher than that achieved by conventional extraction. The HPLC analysis of mangiferin and genkwanin revealed well linearity and stability, with their content level to be 16.9 and 0.204 mg/g, respectively. Additionally, the TF extract significantly alleviated H₂O₂-induced cell death in IPEC-J2 cells by inhibiting the NF-kB/NLRP3/IL-18 signaling pathway. This study provides valuable information for the development and utilization of TF from <em>A. sinensis</em> leaves as a functional ingredient in feed and nutritional health products.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"49 ","pages":"Article 100664"},"PeriodicalIF":3.6,"publicationDate":"2025-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145222400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}