Journal of Applied Research on Medicinal and Aromatic Plants最新文献

{"title":"Optimization of microwave-assisted extraction of antioxidant compounds from Vitex negundo leaves using response surface methodology","authors":"Anindita Kundu ,&nbsp;Niladri Sett","doi":"10.1016/j.jarmap.2025.100659","DOIUrl":"10.1016/j.jarmap.2025.100659","url":null,"abstract":"<div><div>Polyphenolic substances obtained from the leaves of <em>Vitex negundo</em> have gained attention for its therapeutic benefits. To obtain the maximized antioxidant-rich polyphenolic substances from the <em>Vitex negundo</em> leaves via microwave-assisted extraction (MAE), Box–Behnken design (BBD) was employed to evaluate the influence of several variables of MAE on the total phenolic content (TPC) and total flavonoid content (TFC) as determined by Folin–Ciocalteu assay method and Aluminum Chloride assay method, and the antioxidant capacity as measured by DPPH and ABTS assay methods, respectively. Response surface methodology (RSM) was used for finding optimal extraction conditions. GC-MS analysis was conducted on the extract. The tocopherol content and anticancer potential were estimated using HPLC and MTT assay respectively. The ideal extraction parameters were found to be 14 min, 48 °C, 65 % (v/v) methanol concentration, and 14 ml of extraction solvent. The optimum experimental conditions produced the TPC and TFC values of 1.46 mg GAE/g of dried extract and 1.06 mg QE/g of dried extract respectively. Furthermore, the DPPH and ABTS assays results showed the optimum values of 53 % and 77 % respectively. 12 bioactive compounds were identified using GC-MS. The amount of tocopherol was found to be 414.87 µg/g. Lastly, the obtained leaf extract demonstrated its anticancer potential on PC3 cell lines. The findings demonstrated leaf extract's potential as a useful source of polyphenols with strong antioxidant qualities that can be used in a variety of pharmaceutical applications.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"48 ","pages":"Article 100659"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An effective optimization strategy for extracting functional ingredients from Polygonatum cyrtonema Hua based on quality by design principles","authors":"Sijie Zhang ,&nbsp;Mingyang Qiu ,&nbsp;Jiang Wan ,&nbsp;Zheng Qian ,&nbsp;Yingpeng Tong ,&nbsp;Jin-Feng Hu","doi":"10.1016/j.jarmap.2025.100655","DOIUrl":"10.1016/j.jarmap.2025.100655","url":null,"abstract":"<div><div>This study aimed to develop and validate a novel and robust strategy for the simultaneous extraction of the functional ingredients, including phenolic components, polysaccharides, saponins, and amino acids, from <em>Polygonatum cyrtonema</em> Hua using ultrasound-assisted extraction based on quality by design principles. Critical process parameters determined using Ishikawa diagram and systematic risk analyses were investigated through a Box-Behnken design. A Monte-Carlo simulation was subsequently utilized to establish a design space. After verification experiments, chemometric analysis was eventually applied to evaluate the quality consistency of samples from different geographical regions. Models investigating the relationships between process parameters and indicators were successfully established, achieving <em>R</em>² &gt; 0.89. Furthermore, a hypercube design space for process control was successfully established based on constraints of the desired quality attributes. Verification experiments confirmed the accuracy and reliability of both the models and the design space. The validated method effectively extracted active ingredients from <em>P. cyrtonema</em> samples sourced from different geographical regions, allowing their differentiation. This study supports the application of the proposed quality-by-design-optimized extraction method for efficiently recovering active constituents from <em>P. cyrtonema</em>, thus facilitating quality control and providing valuable insights into the sustainable utilization of this traditional Chinese medicine.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"48 ","pages":"Article 100655"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144988626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative predictive power of single-year vs. multi-year BLUPs and YREMs for herbage and essential oil yield in lemongrass (Cymbopogon flexuosus Stapf)","authors":"K.V. Ashwini,&nbsp;S. Gazala Parveen,&nbsp;Channayya Hiremath","doi":"10.1016/j.jarmap.2025.100658","DOIUrl":"10.1016/j.jarmap.2025.100658","url":null,"abstract":"<div><div>Cultivar recommendation for commercial scale cultivation involves evaluating several advanced breeding lines across multiple locations and years to identify the most promising and stable line(s). Theoretically, it is suggested that cultivars’ single-location multiple-year performance data has higher predictive power of their future performance than single year data. In the present study, we attempted to examine this hypothesis in 13 lemongrass clones evaluated across four years (2018–2021) at CSIR-Central Institute of Medicinal and Aromatic Plants Research Centre, Bengaluru, India for herbage and essential oil yield using the statistical predictors <em>viz.,</em> best linear unbiased predictors (BLUP) and yield relative to environment maximum (YREM). Besides, we also identified stable and high herbage and essential oil yielding lemongrass clones using AMMI based stability parameters, mean BLUP and YREMs. The predictive power of single-year <em>vs</em> multi-year data was assessed by comparing the correlation co-efficients of the lemongrass clones’ current year’s performance with preceding 1-, 2- and 3- years’ performance. The results of our study indicated that the multi-year and single-year performance data were adequate to predict the lemongrass’ herbage and essential oil yield, respectively for future years’ performance and to identify superior clones. The clone B3 3–1 exhibited superior herbage and essential oil yield based on mean BLUP and YREM as measures of clones’ performance, besides exhibiting excellent stability as indicated by AMMI-based stability estimates. Further, evaluation of the clone 5 (B2 6–4) for stable herbage and essential oil yield performance across multi-locations shall aid in identifying a cultivar suitable for commercial cultivation.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"48 ","pages":"Article 100658"},"PeriodicalIF":3.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145044523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of the medicinal and aromatic plants value chain in Albania: Challenges and development potentials","authors":"Alban Ibraliu ,&nbsp;Drini Imami ,&nbsp;Xavier Simonnet ,&nbsp;Christoph Carlen ,&nbsp;Andrea Pieroni ,&nbsp;Andrea Primavera ,&nbsp;Zamir Libohova ,&nbsp;Sokrat Sinaj","doi":"10.1016/j.jarmap.2025.100653","DOIUrl":"10.1016/j.jarmap.2025.100653","url":null,"abstract":"<div><div>Albania's rich biodiversity and diverse climatic conditions make it an ideal country for the collection and cultivation of medicinal and aromatic plants (MAPs). With over 400 indigenous MAP species, this sector plays a significant role in the country's economy, generating more than 50 million Euros annually and accounting for about 25 % of total agricultural exports. Moreover, the sector provides employment and income for thousands of rural households. This study examines the MAPs sector trends and patterns in Albania, highlighting strengths, weaknesses, threats and opportunities. Despite the global rise in demand for MAP products, driven by technological advancements and changing consumer attitudes, the Albanian MAPs sector faces challenges related to standards, processing capabilities and market access. Through a combination of qualitative interviews with key stakeholders and analysis, this study offers a comprehensive overview of the sector's current development, identifies critical issues, and provides recommendations for economic and political actors aimed at strengthening the value chain and market competitiveness of Albanian MAPs.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"48 ","pages":"Article 100653"},"PeriodicalIF":3.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144880126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of agronomic and processing factors on bioactive compounds of yerba mate (Ilex paraguariensis A. St.- Hil): A systematic review","authors":"Jéssica de Cássia Tomasi ,&nbsp;Renata de A. Maggioni ,&nbsp;Mônica Moreno Gabira ,&nbsp;Natália Saudade de Aguiar ,&nbsp;Manoela Mendes Duarte ,&nbsp;Leandro Marcolino Vieira ,&nbsp;Cristiane Vieira Helm ,&nbsp;Ivar Wendling","doi":"10.1016/j.jarmap.2025.100652","DOIUrl":"10.1016/j.jarmap.2025.100652","url":null,"abstract":"<div><div>The acquisition of yerba mate (<em>Ilex paraguariensis</em>) premium raw material with particular phytochemical profiles is made possible by understanding the variables influencing its chemical composition. Using PRISMA methodology, we made a systematic review choosing papers from indexed journals that connected yerba mate leaves phytochemical properties to cultivation management and processing variables. We discovered that there are still few studies with this focus, the results present discrepancies and the amount of new research on the subject is increasing. Factors related to planting, management, harvesting, and processing can affect the amount of bioactive compounds found in yerba mate leaves and are key to obtain high-quality raw materials. In order to provide more accurate answers, we emphasize the necessity of conducting new research, identifying factors, particularly genetic ones, standardizing sample collection, and developing analytical techniques. We hope that this review will assist in obtaining research insights and bring the possibility to obtain high-quality chemical-grade yerba mate products that are sufficiently tailored to each consumer market.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"48 ","pages":"Article 100652"},"PeriodicalIF":3.6,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144887139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Temperature-dependent variations in saffron (Crocus sativus L.) quality: A GIS-based suitability analysis of apocarotenoid concentrations","authors":"Mohammad Shahin Daneshmandi ,&nbsp;Behnam Kamkar ,&nbsp;Hassan Rezadoost","doi":"10.1016/j.jarmap.2025.100654","DOIUrl":"10.1016/j.jarmap.2025.100654","url":null,"abstract":"<div><div>The quality of saffron (<em>Crocus sativus</em> L.) stigma is determined through its apocarotenoid content, especially crocin, picrocrocin, and safranal (C-P-S), which affect its dye, flavour and fragrance, respectively. This study provides a GIS-based technique to assess land suitability for saffron cultivation, emphasizing the crucial role of summer temperature in optimizing C-P-S concentrations. Data were collected from 23 saffron fields, where C-P-S content was quantified using High-Performance Liquid Chromatography (HPLC). Long-term temperature data were extracted from TerraClimate database using Google Earth Engine and analyzed in a GIS environment to identify optimal summer temperature ranges. Optimal temperature ranges were established based on conditions observed in the best-performing fields, with deviations classified as suboptimal. These temperature thresholds were used to define climatic and quality zones, resulting in the identification of 34 climatic zones and 71 stigma quality zones across the studied provinces. Results indicate that only 5 % of the total arable land falls within the highest-quality zone, with the most suitable regions located in Razavi Khorasan Province. A strong negative correlation was observed between increasing summer temperatures and declining crocin and picrocrocin levels, demonstrating a temperature-sensitive response. These findings provide a perspective for agricultural decision-makers and support the development of climate-adaptive strategies for saffron cultivation.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"48 ","pages":"Article 100654"},"PeriodicalIF":3.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144880123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Litheness of reproductive traits drives perplexing sexual polymorphism and adaptive strategies in Thymus linearis Benth. across diverse habitats","authors":"Aabid M. Rather ,&nbsp;Bilal A. Wani ,&nbsp;Irshad A. Nawchoo ,&nbsp;Manoj K. Dhar","doi":"10.1016/j.jarmap.2025.100651","DOIUrl":"10.1016/j.jarmap.2025.100651","url":null,"abstract":"<div><div>Plasticity in floral traits is often associated with sexual polymorphism that reflects adaptations of plant species to different ecological conditions. Here, we studied the adaptive significance of sexual polymorphism on the reproductive ecology and reproductive success of <em>Thymus linearis</em> −a gynodiocious and duo-dichogamous plant species from the Kashmir Himalayas. All eight studied sites exhibited the coexistence of both female and hermaphrodite flowers with significantly different reproductive attributes. Female flowers were relatively smaller (0.27 ± 0.01 mm in diameter) compared to hermaphrodite flowers (0.32 ± 0.02 mm). In sexual accomplishment of <em>T. linearis</em>, we identified intervention of four floral morphs (A, B, C, and D) with unique form and functionality. These floral morphs varied in the number of pollens per flower, pollen viability, pollen variation, pollen load index, and stigma receptivity. The maximum pollen number was recorded in morph A (3373.3 ± 21.95) and the minimum in morph C (95.12 ± 0.01). The maximum pollen viability was recorded in morph D (87.66 ± 0.61 %), and the minimum in morph B and C (0 %). The maximum pollen variation was observed in morph D (60.53 ± 1.35) and the minimum in morph A (4.53 ± 0.165). The pollen load index was maximum in morph B (0.75) and minimum in morph A (0.25). The maximum duration of stigma receptivity was recorded in morph B (7–8 days) and the minimum in morphs A and D (2 days). The aforementioned species exhibited mixed mating favouring xenogamy over autogamy. The seed set recorded in xenogamy was 78.23 ± 1.34 and 54.57 ± 0.17 % for both female and hermaphrodite flowers, while in autogamy, the seed set recorded was 0 and 44.57 ± 0.17 % for both the female and hermaphrodite flowers. The species is mainly pollinated by honey bees and bumble bees. The research findings on the reproductive biology and sexual polymorphism of <em>T. linearis</em> will provide valuable insights for the development of effective conservation and breeding strategies.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"48 ","pages":"Article 100651"},"PeriodicalIF":3.6,"publicationDate":"2025-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144880125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of ginger-derived exosome-like nanoparticle isolation: Synergistic effects of polyethylene glycol, filtration, and pH","authors":"Sara Ahmadi Shokooh,&nbsp;Masoud Fereidoni","doi":"10.1016/j.jarmap.2025.100649","DOIUrl":"10.1016/j.jarmap.2025.100649","url":null,"abstract":"<div><div>Plant-derived exosome-like nanoparticles (PELNPs) represent promising bioactive agents, with ginger-derived exosome-like nanoparticles (GELNPs) exhibiting notable therapeutic potential due to their anti-inflammatory, antioxidant, and neuroprotective properties. While polyethylene glycol (PEG)-based extraction offers a cost-effective alternative to ultracentrifugation for PELNP isolation, methodological refinements are needed to enhance yield, stability, and reproducibility. This study systematically optimizes GELNP isolation by integrating PEG6000 precipitation with pH adjustment and 0.45 µm filtration, building upon existing ginger nanovesicle protocols. We present the first comparative evaluation of pH modulation versus filtration, demonstrating distinct trade-offs: pH-adjusted GELNPs achieved smaller size, whereas filtered GELNPs exhibited superior colloidal stability and lower polydispersity index (PDI). Furthermore, we identify previously unreported seasonal variations in GELNP properties, with July-derived nanoparticles displaying enhanced stability and reduced size compared to those isolated in other months. Comprehensive physicochemical characterization—including transmission electron microscopy (TEM), atomic force microscopy (AFM), particle size analysis (PSA), zeta potential analysis, and Fourier-transform infrared spectroscopy (FT-IR)—confirmed differences in morphology, size distribution, surface charge, and biomolecular composition. These findings highlight the necessity for seasonally adjusted, optimized isolation protocols to ensure batch-to-batch consistency and maximize therapeutic efficacy, particularly for drug delivery applications. By addressing critical methodological gaps and introducing temporal considerations, this work establishes a framework for scalable, high-quality GELNP production.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"48 ","pages":"Article 100649"},"PeriodicalIF":3.6,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144771136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of cellulase-assisted hydrodistillation for Homalomena occulta essential oil: A response surface methodology approach","authors":"Ky-Quang Tran Le ,&nbsp;Dai Dinh Dang ,&nbsp;Thao-Tran Thi Nguyen","doi":"10.1016/j.jarmap.2025.100647","DOIUrl":"10.1016/j.jarmap.2025.100647","url":null,"abstract":"<div><div>Species belonging to the genus <em>Homalomena</em> naturally grow in tropical climates, particularly in areas with high humidity, such as the tropical forests of Vietnam. Essential oils extracted from plants in this genus are currently considered to be expensive. This study focused on the rhizome essential oil of <em>Homalomena occulta</em> (Lour.) Schott., collected in Gia Lai province, and aimed to optimize the extraction process through cellulase-assisted hydrodistillation. Using response surface methodology (RSM) with a central composite design (CCD), the study evaluated the effects of three factors—pH level, enzyme concentration, and incubation time—on the extraction yield. The optimal conditions identified were a cellulase concentration of 0.25 % at a pH of 3.5 for an incubation period of 5 h. Under these conditions, there was a three-fold increase in yield (0.321 ± 0.016 %) compared to the control (0.100 ± 0.011 %). When comparing essential oil samples extracted without cellulase to those extracted using cellulase under optimal conditions, it was evident that the oils obtained from cellulase-treated materials had a higher yield without compromising quality. The organoleptic and physical properties remained largely unchanged; the essential oil retained a transparent, pale yellow color and a sweet, fresh aroma. However, the sample from the cellulase-treated material had a stronger woody scent. The physical properties demonstrated minimal variation, with specific gravities of 0.877 ± 0.0026 g/cm³ and 0.888 ± 0.0016 g/cm³ , respectively. The refractive indices were 1.4671 ± 0.00020 and 1.4680 ± 0.00046, while the optical rotation angles changed insignificantly (-10.388 ± 0.3506 vs. −9.271 ± 0.1851). The essential oil from <em>Homalomena</em> remains rich in natural aromatic compounds, including linalool and various oxygenated compounds such as terpinene-4-ol, α-terpineol, epi-α-muurol, and α-cadionol, all of which have biological value and potential applications.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"48 ","pages":"Article 100647"},"PeriodicalIF":3.8,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144704662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supercritical fluid extraction of Bidens pilosa L. oil: Kinetics evaluation and antitumor activity","authors":"Ramon G. Paschoal ,&nbsp;Marianne L. Higino ,&nbsp;Ana Paula G. Alves ,&nbsp;André Luiz F. Sampaio ,&nbsp;Dulcineia F. Teixeira ,&nbsp;Leonardo Lucchetti ,&nbsp;Marisa F. Mendes","doi":"10.1016/j.jarmap.2025.100641","DOIUrl":"10.1016/j.jarmap.2025.100641","url":null,"abstract":"<div><div>Different plants have been used, around the world, in the treatment of primary diseases as an alternative or popular medicine. In Brazil, the Health Minister introduced a national politic for the phytotherapy use in the Single Health System (SUS). Due to that, <em>Bidenspilosa</em> L. (Asteraceae) was chosen because it is one of the medicinal plants present in the Single Health System list, called RENISUS. <em>Bidens pilosa</em> L. is a popular plant used in the treatment of different diseases (malaria, cancer tumors, fever, diabetes, infections, inflammations, angina, etc.) and is widely disseminated throughout the tropical and subtropical regions of the world. The biological activities of the plant are attributed to the presence of flavonoids, polyacetylenes and terpenes. Therefore, this study evaluated the biological activities of the oil extraction using supercritical CO₂ at different pressure and temperature conditions, due to the scarcity of studies comparing supercritical fluid extraction to conventional techniques (Soxhlet and maceration). The experimental procedure used 8 g of previously dried and milled leaves at different conditions according to an experimental design (150 bar - 60 °C; 179 bar - 46 °C; 179 bar - 74 °C; 250 bar - 40 °C; 250 bar - 80 °C; 321 bar - 46 °C; 321 bar - 74 °C, 350 bar - 60 °C, 250 bar - 60 °C). According to the results, the best yield was 0.082 % (6.6 mg) at 321 bar and 74 °C. The analyses of the experiments done at 321 bar at 74 °C and 250 bar at 40 °C, showed ultraviolet spectra characteristic of terpenes. The extracts analyzed showed promising inhibition in the two tumor cell lines: melanoma, SK-MEL-28 (68 %) and leukemia K562 (90 %), both at a concentration of 150 μg/mL. The results suggested the importance of the bioactive components from <em>Bidens pilosa</em> to contribute to a new anticancer drug using supercritical technology.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"47 ","pages":"Article 100641"},"PeriodicalIF":3.8,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144280186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}