Journal of Applied Research on Medicinal and Aromatic Plants最新文献

{"title":"Enhancing germination and growth in Malania oleifera Chun & S. K. Lee seeds through gibberellic acid priming","authors":"Xuelin Su ,&nbsp;Xuying Wang ,&nbsp;Nan Cui ,&nbsp;Hailing Xu ,&nbsp;Ting Tian ,&nbsp;Bo Wei","doi":"10.1016/j.jarmap.2025.100629","DOIUrl":"10.1016/j.jarmap.2025.100629","url":null,"abstract":"<div><div><em>Malania oleifera</em> is an economically and medicinally important endangered forest tree endemic to the karst region of southwestern China, known for the high neurotic acid content of its seed oil. However, it faces challenges of low germination and survival rates due to its dormancy characteristics and high fatty acid content (prone to mildew). This study examines the impact of gibberellic acid (GA₃) on <em>M. oleifera</em> seed germination and the associated physiological and biochemical responses. The results showed that the freshly harvested seeds of <em>M. oleifera</em> remained high (78.33 %) viability but germination was low (50 %) after 5 months of storage. 600 mg/L GA₃ significantly increased the seed germination index, but had no effect on the mildew rate, while 400 mg/L GA₃ reduced the mildew rate. Soluble sugar content was inversely related to GA₃ concentration, while soluble protein levels varied, reaching a minimum at 600 mg/L. Antioxidant (SOD, CAT, POD) and starch-degrading enzyme (α-amylase and β-amylase) activities peaked at this GA₃ concentration. The soluble protein level under 600 mg/L GA₃ treatment was lowest at early stage (S1) and soluble sugar level was lowest at late stage (S2). Antioxidant enzyme and starch-degrading enzyme activities increased with germination, peaking at the final stage (S3). In summary, under the 600 mg/L GA₃ treatment, germination parameters, antioxidant enzymes, and starch - degrading enzymes in <em>M. oleifera</em> seeds were all maximized. Further observation of the four germination stages revealed significant changes in nutrients and enzyme activities, accelerating nutrient consumption and enhancing antioxidant defense.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"45 ","pages":"Article 100629"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143453450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recycling of residual biomass of medicinal and aromatic plants: A valuable alternative towards bio-refinery","authors":"Rohan Sarkar ,&nbsp;B.B. Basak ,&nbsp;Atanu Banerjee ,&nbsp;Elias Mosaffa ,&nbsp;Arpan Bhowmik","doi":"10.1016/j.jarmap.2025.100624","DOIUrl":"10.1016/j.jarmap.2025.100624","url":null,"abstract":"<div><div>A large amount of residual waste biomass is generated from medicinal and aromatic plants (MAPs) sector that needs to be utilized through effective recycling as resources and to reduce environmental load. In this study, identification of functional groups, surface morphology, nature of crystallinity, thermal property, biochemical (cellulose, hemicellulose and lignin), and elemental profiling (macro and micro nutrients) was carried out in residual biomasses of six aromatic and two medicinal plants to understand their best alternate use as different bio-refinery potential such as bioethanol, biochar, compost, bioactive rich extract, and growth medium. The biochemical and elemental profile provided quantitative data for primary grouping of the biomasses based on their possible usage that was supported by qualitative (or semi-quantitative) data from functional, morphological characterization, analysis of crystallinity and thermal behaviour. The biomass of MAPs like lemongrass, palmarosa, java citronella, isabgol and ashwagandha had higher cellulose and hemicellulose content (structural, crystalline and biochemical information), make them useful for preparing bioethanol. On the other hand, biomasses with high aromaticity and thermal stability in their structure like- holy basil, sweet basil, menthol mint and isabgol, found suitable towards development of biochar. Elemental profile showed suitability of holy basil, sweet basil, menthol mint and isabgol biomass for their composting potential. All biomasses seemed to be useful for growth medium preparation based on biochemical, functional and morphological analysis. Amalgamation of different characterization techniques were found rational to understand the best alternative(s) for residual biomasses. Thus, this study helps to develop a strategy for complete recycling of biomasses under model bio-refinery, which also advocate circular economy.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"45 ","pages":"Article 100624"},"PeriodicalIF":3.8,"publicationDate":"2025-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143168850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Findings on the heavy metal content in herbal drugs and essential oils – An updated database evaluation","authors":"Gerald Binder ,&nbsp;Matthias Knödler ,&nbsp;Bernhard Klier ,&nbsp;Patricia Zang ,&nbsp;Hagen Albert ,&nbsp;Alexandra Barofsky ,&nbsp;Barbara Steinhoff","doi":"10.1016/j.jarmap.2024.100609","DOIUrl":"10.1016/j.jarmap.2024.100609","url":null,"abstract":"<div><div>In order to guarantee the safety of patients and consumers, the European legal framework restricts the content of heavy metals and further elements in plant material used for the production of medicinal and food products. This data evaluation comprises findings on seven elements between 2016 and 2021 which were assessed against the requirements of the European Pharmacopoeia (Ph.Eur.) and the ICH Q3D guideline on elemental impurities. !5.328 herbal drug samples from 427 products and 537 essential oil samples from 25 products were included in this evaluation which demonstrates that the Ph. Eur. limits set for cadmium, lead and mercury are considered appropriate. Samples from organic production revealed a lower occurrence of cadmium, lead and mercury as compared to samples from conventional production. With regard to the ICH Q3D requirements, none of the herbal drug samples exceeded the respective limit for cobalt, nickel and vanadium<em>,</em> while for arsenic higher values were observed in individual cases. A comparison of the findings with a former period of evaluation showed an increase in positive findings and also a few more exceedances of the Ph.Eur. limits. For essential oils, it can be confirmed that independent from the production process, the probability of heavy metal contamination is low.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"44 ","pages":"Article 100609"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142747858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimized flow cytometry protocol for assessing DNA content in Vanilla (Orchidaceae) species: Insights into crop wild relatives and a commercial hybrid","authors":"Jorge Warner ,&nbsp;Yesly Camacho-Solís ,&nbsp;Víctor M. Jiménez ,&nbsp;Alfonso García-Piñeres","doi":"10.1016/j.jarmap.2024.100612","DOIUrl":"10.1016/j.jarmap.2024.100612","url":null,"abstract":"<div><div>Vanilla (Orchidaceae) is a highly valued spice used in a wide range of products. Although wild crop relatives of <em>Vanilla planifolia</em>, the primary cultivated species, may possess traits for crop improvement, they have received limited attention. This study presents an optimized and reproducible flow cytometry protocol adapted to <em>Vanilla</em> leaves, that effectively prevents nuclei clustering and raphide interference. Assessing nuclear C-DNA content and estimating DNA ploidy in succulent plant tissues pose significant challenges. The abundance of polysaccharides and raphides in <em>Vanilla</em> tissues often leads to inaccuracies in cytometric measurements and difficulties in instrument maintenance. To address this issue, we used a nuclei isolation buffer containing 2.0 % (v/v) detergent and utilized the clear upper phase of the filtrate after raphide sedimentation to obtain accurate nuclear DNA content values. We applied this protocol to estimate the C-DNA content of 12 <em>Vanilla</em> species native to Costa Rica and a widely cultivated commercial hybrid. The values ranged from 5.211 ± 0.008 pg in <em>V. planifolia</em> to 9.0 ± 1.3 pg in <em>V. pompona</em>, with no clear distinction between species of the subgenera <em>Vanilla</em> and <em>Xanata</em>. For the first time, we report the 2C-DNA content of <em>V. costaricensis</em>, <em>V. dressleri</em>, <em>V. hartii</em>, <em>V. helleri</em>, <em>V. inodora</em>, <em>V. karen-christianae</em> and the 'Vaitsy' hybrid. Our results confirm the occurrence of partial endoreplication in all samples analyzed. This study contributes to the understanding of less studied wild crop relatives of <em>V. planifolia</em> and provides an improved method for flow cytometric analysis that can be applied to tissues containing mucilaginous compounds and raphides of other plant species.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"44 ","pages":"Article 100612"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143164541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of genetic diversity and population structure of Pinellia ternata using EST-SSR markers","authors":"Mingxing Wang ,&nbsp;Rong Xu ,&nbsp;Ming Luo ,&nbsp;Xinyao Li ,&nbsp;Lu Wei ,&nbsp;Mi Lei ,&nbsp;Dahui Liu ,&nbsp;Yuhuan Miao","doi":"10.1016/j.jarmap.2024.100611","DOIUrl":"10.1016/j.jarmap.2024.100611","url":null,"abstract":"<div><div><em>Pinellia ternata</em> (Thunb.) Breit. is a widely used Chinese Medicinal Material with economic and medicinal value. Few molecular studies have been conducted on <em>P. ternata</em>, resulting in a lack of applicable molecular markers. This present research analyzed the genetic diversity of 255 <em>P. ternata</em> germplasm resources from China, including 117 wild germplasm resources and 138 cultivated germplasm resources. Following the development of 17 pairs of highly polymorphic primers, 126 alleles were found; the average polymorphism was 99.21 %. The average number of observed alleles (<em>Na</em>), effective alleles (<em>Ne</em>) were 1.98 and 1.36, respectively. The mean of the Nei genetic diversity index (<em>H</em>), the Shannon's information index (<em>I</em>) and the polymorphic information content (<em>PIC</em>) were 0.23, 0.36 and 0.6482, respectively. The population structure of <em>P. ternata</em> germplasm resources was categorized into two groups, designated as Group 1 and Group 2. The majority of wild germplasm resources was clustered in Group 1. Phylogenetic and population structure analyses produced similar results. A comparison of the genetic diversity parameters of wild and cultivated germplasm revealed that the genetic diversity was higher in the former, while the genetic differentiation level of the two types of germplasm was low. Analysis of molecular variance (AMOVA) demonstrated that 9 % of the genetic variation was between populations and 91 % of the genetic variation was within populations. Principal coordinate analysis (PCoA) served to corroborate the findings of the cluster and population structure analysis. It was found that <em>P. ternata</em> with tubers having red epidermis was more separated from other germplasm resources. Association analysis based on the generalized linear model (GLM) detected four EST-SSR markers significantly associated with <em>P. ternata</em> tuber color. This study will be helpful for <em>P. ternata</em> germplasm resource conservation, identification and trait-specific breeding programs.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"44 ","pages":"Article 100611"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of genetic diversity and construction of core germplasm in populations of Acorus tatarinowii based on SNP markers","authors":"Qian Qin,&nbsp;Yanjing Dong,&nbsp;Jiaqi He,&nbsp;Jialong Chen,&nbsp;Danyang Wu,&nbsp;Shouwen Zhang","doi":"10.1016/j.jarmap.2024.100605","DOIUrl":"10.1016/j.jarmap.2024.100605","url":null,"abstract":"<div><div><em>Acorus tatarinowii</em> is a natural medicinal plant integral to traditional aromatic therapies. It is commonly employed in the treatment of depression, epilepsy, and Alzheimer's disease due to its significant medicinal and aromatic properties. However, the genetic diversity of wild <em>A. tatarinowii</em> resources has declined due to over-exploitation and habitat destruction. This study aims to assess the genetic diversity of the natural populations of <em>A. tatarinowii</em>, establish a core germplasm bank, explore its genetic richness and uniqueness, prevent genetic erosion, and identify beneficial genes. In this study, for the first time, 429A. tatarinowii samples from 40 populations were analyzed for genetic diversity and population structure using Hyper-Seq technology. A total of 4772,850 high-quality Single Nucleotide Polymorphisms (SNPs) and 1563,000 Insertions and Deletions (InDels) variant loci were identified, with C/T as the predominant variant type and a Ts/Tv ratio of 1.079. Annotation of these loci indicated that the majority of variants occurring in intergenic regions, accounting for 50.59 % of the total. Moreover, the heterozygosity, nucleotide diversity, and FST of <em>A. tatarinowii</em> suggested low genetic diversity within this species within the populations. The analysis of molecular variance (AMOVA) revealed that the population variation of <em>A. tatarinowii</em> is mainly caused by the variation between populations (72.06 %), while the variation within populations only contributes a small part (27.94 %) Through NJ tree, PCA, and ADMIXTURE analyses, the 429<em>A. tatarinowii</em> samples were classified into five subgroups, with some genetic exchange observed. A total of 7163 high-quality polymorphic SNPs were identified, and a core germplasm consisting of 85 samples was established, achieving genotype retention rates similar to those of the original germplasm. This indicates that a smaller number of germplasm resources can effectively represent the majority of the genetic diversity. Additionally, PCA analysis further confirmed the representativeness and validity of the constructed core germplasm resources. Furthermore, the DNA fingerprints of the 429 accessions were established using the most effective combinations of 26 SNP markers, which served as specific markers to effectively distinguish all samples. In conclusion, these findings offer valuable insights into the genetic structure of <em>A. tatarinowii</em>, facilitating the identification of high-quality genes and providing a scientific foundation for the development of breeding programs and conservation strategies for <em>A. tatarinowii</em>.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"44 ","pages":"Article 100605"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142723020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of the extraction of bioactive compounds from the leaves of Campomanesia sessiliflora (O. Berg) Mattos: Standardizing the utilization of a new resource","authors":"Raquel Parra Loureiro ,&nbsp;Thiago Luis Aguayo de Castro ,&nbsp;Claudia Andrea Lima Cardoso","doi":"10.1016/j.jarmap.2024.100615","DOIUrl":"10.1016/j.jarmap.2024.100615","url":null,"abstract":"<div><div>The use of medicinal plants that produce a secondary metabolite continues to grow along with population growth. One of these species is <em>Campomanesia sessiliflora</em> (O. Berg) Mattos. The literature reports that <em>C. sessiliflora</em> is rich in phenolic compounds and flavonoids, which possess high anti-aging activity and can be utilized in the cosmetic and food industries. Optimizing the extraction of bioactive compounds from this plant is crucial for its industrial application. Currently, <em>Campomanesia sessiliflora</em> leaves are discarded as pruning waste. The evaluation of secondary metabolites from C. sessiliflora leaves is essential to making their use feasible for obtaining antioxidant and photoprotective extracts. This study aims to optimize the extraction of phenolic compounds, flavonoids, tannins, chlorophyll α, chlorophyll β, and carotenoids, as well as evaluate the antioxidant potential, sun protection factor (SPF), and the ultraviolet region scans of the leaf extracts. Liquid chromatography with a diode array detector was used to identify chemical compounds. The extracts were evaluated using different plant-to-solvent ratios and varying ethanol concentrations in water. A correlation test was conducted between the spectrophotometric profile and the levels of phenolic compounds, flavonoids, tannins, and the antioxidant potential of <em>C. sessiliflora</em> leaf extracts. Results showed a positive correlation between ethanol levels and the presence of chlorophyll α, chlorophyll β, carotenoids, and flavonoids. Additionally, a correlation was found between antioxidant potential, SPF, and phenolic compound content. Statistical analyses indicate that the ethanol concentration in water is more significantly relevant than the different plant-to-solvent proportions. Gallic acid, syringic acid, catechin, epicatechin, and rutin were identified in all samples. Absorption at the 260 nm wavelength can be used for extract quality control. Regarding the monitored parameters, the best extract was obtained using 2 % leaves in 25 % ethanol in water.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"44 ","pages":"Article 100615"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165151","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Morphological and physiological plasticity of alpine medicinal plants along an elevational gradient","authors":"Xuemei Wu ,&nbsp;Liwen Zhong ,&nbsp;Guopeng Chen ,&nbsp;Shihong Zhong ,&nbsp;Rui Gu","doi":"10.1016/j.jarmap.2024.100613","DOIUrl":"10.1016/j.jarmap.2024.100613","url":null,"abstract":"<div><div>Alpine medicinal plant growth, physiological processes and final number of metabolites are affected by elevation, demonstrating phenotypic plasticity. Therefore, in-depth understanding the alpine medicinal plant behaviors in responding to shift elevation has actually implications for resources protection, sustainable utilization and domestication cultivation. Currently, lots of literature detailly studied the effect of environmental factors on the synthesis and metabolism of second metabolisms in medicinal plants while the responding results of phenotype and metabolism to constrain environmental still lack systematic classification and summary. Hence, basing on previous works and literature, the adjustment of morphology and metabolism of alpine medicinal plants in responding of shift elevation were systematically outlined. The results of this reviews can know how the alpine plant adapting to shift elevation through physiological, metabolic and morphological adaptation. And this knowledge can be integrated into a framework for domesticating and protecting alpine medicinal plants, and can also serve as a quick tool to study the effects of climate warming on plateau medicinal plants.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"44 ","pages":"Article 100613"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of gibberellic acid treatment and cold stratification on breaking combined (physiological + mechanical) dormancy and germination in Sassafras tzumu (Hemsl.) Hemsl seeds","authors":"Yu Wu ,&nbsp;Zhi Yun Deng ,&nbsp;Ming Zhu Wang ,&nbsp;Lin Yue Liu ,&nbsp;Yong Bao Shen","doi":"10.1016/j.jarmap.2024.100606","DOIUrl":"10.1016/j.jarmap.2024.100606","url":null,"abstract":"<div><div><em>Sassafras tzumu</em> (Hemsl.) Hemsl., known as Chinese sassafras, is an exclusive species of tree found in China, belonging to the <em>Sassafras</em> genus in the Lauraceae family. Its primary mode of propagation is through seeds, which possesses a profound dormancy and irregular germination. In its natural environment, it takes approximately 2–3 years for the seeds to emerge from the ground and begin germinating. Consequently, a rapid and effective method for breaking dormancy would greatly benefitsbn edjhn the production of <em>S. tzumu</em> seedlings. The objective of this investigation was to identify the optimal treatment for breaking dormancy. The <em>S. tzumu</em> seeds were subjected to gibberellic acid (GA₃) treatment and cold stratification. The findings indicated that the dormancy type of <em>Sassafras tzumu</em> seeds was combined (physiological + mechanical) dormancy. After 6 months of cold stratification, the germination percentage of seeds treated with various concentrations of GA₃ (200 mg/L, 500 mg/L, and 1000 mg/L) all exceeded 50 %. Among these treatments, the most effective method was the application of 200 mg/L GA₃ in combination with 5 months of cold stratification, resulting in a germination percentage of 55 %. During the process of cold stratification, soluble proteins, soluble sugars, starch, and fat were broken down into smaller molecules to support embryo growth. Interestingly, the content of soluble sugar exhibited an upward-downward trend, while the content of soluble protein displayed a downward-upward trend. The release of dormancy in <em>S. tzumu</em> seeds was jointly regulated by various endogenous hormones. The content of GA₃ demonstrated an upward-downward trend, while the ABA content exhibited a continuous decline with a brief upward trend. In contrast, the IAA content displayed a continuous upward trend, and the ZR content showed no significant change. The ratios of GA₃/ABA, IAA/ABA, and ZR/ABA all displayed an upward trend.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"44 ","pages":"Article 100606"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143165163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular authentication of Paeonia species for paeonia radix production using plastid and nuclear DNA markers","authors":"Jiseok Kim ,&nbsp;Jong-Soo Kang ,&nbsp;Hyun-Seung Park ,&nbsp;Jae-Hyeon Jeon ,&nbsp;Jee Young Park ,&nbsp;Eunbi Yeo ,&nbsp;Jung Hwa Kang ,&nbsp;Seung Hyun Kim ,&nbsp;Do Won Jeong ,&nbsp;Young-Sik Kim ,&nbsp;Hocheol Kim ,&nbsp;Woojong Jang ,&nbsp;Goya Choi ,&nbsp;Byeong Cheol Moon ,&nbsp;Tae-Jin Yang","doi":"10.1016/j.jarmap.2024.100604","DOIUrl":"10.1016/j.jarmap.2024.100604","url":null,"abstract":"<div><div><em>Paeonia lactiflora</em> is a perennial herbaceous plant popular in oriental medicine and as an ornamental plant. Two related species, <em>P. veitchii</em> and <em>P. obovata</em>, share several medicinal uses with <em>P. lactiflora</em>. To develop a molecular authentication system for these three <em>Paeonia</em> species, which are used to produce the Chinese herbal medicine Paeonia Radix, we generated super-barcode information. This included complete plastid genomes (plastomes) and 45S nuclear ribosomal DNA (45S nrDNA) from two accessions of <em>P. lactiflora</em> and one accession each of <em>P. veitchii</em> and <em>P. obovata</em>, using whole-genome sequencing data. The four plastomes range from 152,519 to 152,731 base pairs (bp) in length and have the same gene content and order. The 45S nrDNA transcriptional units range from 5879 to 6315 bp in length and show 33 SNPs among the three species. Phylogenetic analyses based on plastome and 45S nrDNA sequences clearly identified each species as a monophyletic group. Inter- and intraspecific polymorphisms were discovered among these three <em>Paeonia</em> species and <em>Paeonia suffruticosa</em>. Four molecular markers were developed to discriminate between <em>P. lactiflora</em>, <em>P. veitchii</em>, <em>P. obovata</em>, and <em>P. suffruticosa</em>, the main species used to produce Paeonia Radix in Korea and China. These markers successfully authenticated the species when applied to multiple herbal samples collected from various origins.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"44 ","pages":"Article 100604"},"PeriodicalIF":3.8,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142723019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}