Influence of seed pre-treatment and storage on germination and physiological characteristics of seeds of common mullein (Verbascum thapsus L.)

IF 3.8 2区 农林科学 Q1 PLANT SCIENCES
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Abstract

We report the seed germination and associated metabolic responses of differentially stored seeds of Verbascum thapsus L. (Scrophulariaceae), an economically and medicinally important herb from the cold desert region of Lahaul (Himachal Pradesh, India) in trans Himalaya, to certain physico-chemical and GA₃ treatments. The freshly harvested seeds of V. thapsus exhibited high (96 %) viability but low germination (55 %). During the storage of seeds under ambient conditions, viability did not change until 1-year but declined progressively thereafter showing a 33 % decline in 4-year stored seeds. The seed germination was promoted to varying extents by acid scarification (AS), SNP (sodium nitroprusside), SHC (sodium hypochlorite) and GA₃ pre-treatments. Storage-dependent changes in seed germination performance and responsiveness to different effectors were evident. Germination performance improved significantly due to 1-year storage followed by a sharp decline subsequently. The responsiveness of seeds to various treatments tended to be higher in seeds stored for ≥2 years. The changes in germination were paralleled by those in α-amylase activity in seeds. Lipid peroxidation generally increased during storage that was paralleled by a gradual decline in superoxide dismutase (SOD) activity and phenolic contents. Catalase (CAT) activity increased transiently in 1-year stored seeds and declined thereafter. Data are expected to have implications for multiplication and cultivation of V. thapsus.

种子预处理和储藏对毛蕊花种子萌发和生理特性的影响
我们报告了喜马拉雅山脉拉豪尔(印度喜马偕尔邦)寒冷沙漠地区的一种具有重要经济和药用价值的草本植物--Verbascum thapsus L.(毛莨科)的不同储藏方式的种子在某些物理化学和 GA₃处理下的萌发及相关代谢反应。刚收获的毛地黄种子显示出很高的存活率(96%),但发芽率较低(55%)。种子在环境条件下贮藏期间,存活率在 1 年前没有变化,但此后逐渐下降,贮藏 4 年的种子存活率下降了 33%。酸洗(AS)、SNP(硝普钠)、SHC(次氯酸钠)和 GA₃预处理在不同程度上促进了种子的萌发。种子的萌发性能和对不同效应因子的反应随储藏而发生明显变化。种子贮藏 1 年后,萌发性能明显提高,但随后急剧下降。贮藏≥2 年的种子对各种处理的反应性往往更高。种子α-淀粉酶活性的变化与发芽率的变化同步。在贮藏过程中,脂质过氧化通常会增加,同时超氧化物歧化酶(SOD)活性和酚含量也会逐渐下降。过氧化氢酶(CAT)活性在贮藏 1 年的种子中短暂上升,随后下降。预计这些数据将对毛蕊花的繁殖和栽培产生影响。
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来源期刊
Journal of Applied Research on Medicinal and Aromatic Plants
Journal of Applied Research on Medicinal and Aromatic Plants Pharmacology, Toxicology and Pharmaceutics-Drug Discovery
CiteScore
6.40
自引率
7.70%
发文量
80
审稿时长
41 days
期刊介绍: JARMAP is a peer reviewed and multidisciplinary communication platform, covering all aspects of the raw material supply chain of medicinal and aromatic plants. JARMAP aims to improve production of tailor made commodities by addressing the various requirements of manufacturers of herbal medicines, herbal teas, seasoning herbs, food and feed supplements and cosmetics. JARMAP covers research on genetic resources, breeding, wild-collection, domestication, propagation, cultivation, phytopathology and plant protection, mechanization, conservation, processing, quality assurance, analytics and economics. JARMAP publishes reviews, original research articles and short communications related to research.
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