{"title":"Influence of seed pre-treatment and storage on germination and physiological characteristics of seeds of common mullein (Verbascum thapsus L.)","authors":"","doi":"10.1016/j.jarmap.2024.100573","DOIUrl":null,"url":null,"abstract":"<div><p>We report the seed germination and associated metabolic responses of differentially stored seeds of <em>Verbascum thapsus</em> L. (Scrophulariaceae), an economically and medicinally important herb from the cold desert region of Lahaul (Himachal Pradesh, India) in trans Himalaya, to certain physico-chemical and GA₃ treatments. The freshly harvested seeds of <em>V. thapsus</em> exhibited high (96 %) viability but low germination (55 %). During the storage of seeds under ambient conditions, viability did not change until 1-year but declined progressively thereafter showing a 33 % decline in 4-year stored seeds. The seed germination was promoted to varying extents by acid scarification (AS), SNP (sodium nitroprusside), SHC (sodium hypochlorite) and GA₃ pre-treatments. Storage-dependent changes in seed germination performance and responsiveness to different effectors were evident. Germination performance improved significantly due to 1-year storage followed by a sharp decline subsequently. The responsiveness of seeds to various treatments tended to be higher in seeds stored for ≥2 years. The changes in germination were paralleled by those in α-amylase activity in seeds. Lipid peroxidation generally increased during storage that was paralleled by a gradual decline in superoxide dismutase (SOD) activity and phenolic contents. Catalase (CAT) activity increased transiently in 1-year stored seeds and declined thereafter. Data are expected to have implications for multiplication and cultivation of <em>V. thapsus</em>.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":null,"pages":null},"PeriodicalIF":3.8000,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Research on Medicinal and Aromatic Plants","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2214786124000469","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
We report the seed germination and associated metabolic responses of differentially stored seeds of Verbascum thapsus L. (Scrophulariaceae), an economically and medicinally important herb from the cold desert region of Lahaul (Himachal Pradesh, India) in trans Himalaya, to certain physico-chemical and GA₃ treatments. The freshly harvested seeds of V. thapsus exhibited high (96 %) viability but low germination (55 %). During the storage of seeds under ambient conditions, viability did not change until 1-year but declined progressively thereafter showing a 33 % decline in 4-year stored seeds. The seed germination was promoted to varying extents by acid scarification (AS), SNP (sodium nitroprusside), SHC (sodium hypochlorite) and GA₃ pre-treatments. Storage-dependent changes in seed germination performance and responsiveness to different effectors were evident. Germination performance improved significantly due to 1-year storage followed by a sharp decline subsequently. The responsiveness of seeds to various treatments tended to be higher in seeds stored for ≥2 years. The changes in germination were paralleled by those in α-amylase activity in seeds. Lipid peroxidation generally increased during storage that was paralleled by a gradual decline in superoxide dismutase (SOD) activity and phenolic contents. Catalase (CAT) activity increased transiently in 1-year stored seeds and declined thereafter. Data are expected to have implications for multiplication and cultivation of V. thapsus.
期刊介绍:
JARMAP is a peer reviewed and multidisciplinary communication platform, covering all aspects of the raw material supply chain of medicinal and aromatic plants. JARMAP aims to improve production of tailor made commodities by addressing the various requirements of manufacturers of herbal medicines, herbal teas, seasoning herbs, food and feed supplements and cosmetics. JARMAP covers research on genetic resources, breeding, wild-collection, domestication, propagation, cultivation, phytopathology and plant protection, mechanization, conservation, processing, quality assurance, analytics and economics. JARMAP publishes reviews, original research articles and short communications related to research.