Journal of Applied Research on Medicinal and Aromatic Plants最新文献

{"title":"Development of polymorphic simple sequence repeat markers in Agastache rugosa and their application in genetic evaluation and cross-taxon transferability of Agastache species","authors":"Hye Ran Shin ,&nbsp;Ick Hyun Jo ,&nbsp;Raveendar Sebastin ,&nbsp;Jinsu Gil ,&nbsp;Gi Yeong Kim ,&nbsp;Suyun Moon ,&nbsp;Hyun-Seung Park ,&nbsp;Sewon Oh ,&nbsp;Jong Won Han ,&nbsp;Kyung Ho Ma ,&nbsp;Yi Lee ,&nbsp;Daeil Kim ,&nbsp;Ju-Kyung Yu ,&nbsp;Yoon-Sup So ,&nbsp;Jong Wook Chung","doi":"10.1016/j.jarmap.2023.100519","DOIUrl":"10.1016/j.jarmap.2023.100519","url":null,"abstract":"<div><p><span><em>Agastache</em></span><span><span> is a genus of perennial </span>herbaceous plants belonging to the mint family, </span><span><em>Lamiaceae</em></span>. Several <em>Agastache</em><span><span> species are commercially cultivated and used as medicinal, culinary, and ornamental plants. However, information on the </span>genetic diversity and population structure of the species remains unclear. In the present study, genetic diversity within </span><em>Agastache</em><span> species was analyzed using simple sequence repeat (SSR) markers. In this study, 249,746 SSRs were identified in the </span><em>A. rugosa</em><span><span> genome and primer pairs were designed for 56,675 SSRs. The majority of SSR repeat types were dinucleotides (60.65%), followed by </span>trinucleotides (12.38%), and pentanucleotides (12.10%). PCR conditions were established for 250 primer pairs, 111 of which were found to be polymorphic in </span><em>A. rugosa</em><span> germplasm. The number of alleles (N</span>_A) ranged from 2 to 19, major allele frequency (M_AF<span>) ranged from 0.11 to 0.95, observed heterozygosity (H</span>_O) ranged from 0 to 0.89, and polymorphic information content (PIC) ranged from 0.09 to 0.92. Cross-species amplification of SSRs markers in other <em>Agastache</em> species showed amplification rates of 82.6% for <span><em>A. </em><em>foeniculum</em></span> and 78.1% <em>in A. urticifolia</em>, with an average of 80.37%. Cluster analysis of the 19 <em>A. rugosa</em> accessions using SSRs markers revealed four major clusters, and population STRUCTURE analysis using 79 SSRs markers revealed three groups and three subgroups among the <em>A. rugosa</em> populations. The SSRs markers developed can contribute to applications such as varietal identification, genetic diversity analysis, and population structure analysis of <em>A. rugosa</em> germplasm.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"38 ","pages":"Article 100519"},"PeriodicalIF":3.9,"publicationDate":"2023-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136009816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection and molecular characterization of Copamyntis obliquifasciella (Hampson, 1896) infesting medicinal plant, Cassia fistula L from India","authors":"K.T. Shivakumara ,&nbsp;M.C. Keerthi ,&nbsp;P.R. Shashank ,&nbsp;J. Komal ,&nbsp;A.C. Polaiah ,&nbsp;R.S. Ramya ,&nbsp;T. Venkatesan ,&nbsp;D. Sagar ,&nbsp;Ryan Casini ,&nbsp;Ihab M. Moussa ,&nbsp;Hosam O. Elansary ,&nbsp;Ahmed M. El-Sabrout","doi":"10.1016/j.jarmap.2023.100517","DOIUrl":"https://doi.org/10.1016/j.jarmap.2023.100517","url":null,"abstract":"<div><p><span><em>Cassia </em><em>fistula</em></span><span> L. is a valuable medicinal plant<span><span> utilized in many traditional medical systems, including Ayurveda and </span>Oriental medicine system. </span></span><em>Copamyntis obliquifasciella</em><span> Hampson (Lepidoptera: Pyralidae) is a polyphagous insect pest, however the available literature of this pest on </span><em>C. fistula</em><span> is limited or rather scarce in India as well as world. Considering the economic importance of this medicinal plant, documentation of insects pests along with the bio-ecology and natural enemy fauna is important for formulating the integrated pest management program. The present study describes the incidence, damage and life cycle of </span><em>C</em>. <em>obliquifasciella</em> on <em>C. fistula</em><span> in India. The identity of the pest was confirmed through the examination of morphological characteristics<span> of male and female genitalia, further confirmed through molecular analysis (GenBank no. MK559413). The phylogenetic analysis revealed that the Indian population of </span></span><em>C. obliquifasciella</em><span> shared a close relationship with the populations from Pakistan and China. The initial instar larvae of </span><em>C. obliquifasciella</em> is feed by nibbling and scraping the chlorophyll content of the leaves, and later they stitch two or three leaves together and fed in between those stitched leaves. The percent pest incidence varied between 20 and 100, and the foliar damage of 5.5–71% was recorded. The total life cycle of <em>C. obliquifasciella</em><span> was completed in 31.20 ± 2.14 (male) and 34.25 ± 2.36 (female) days. Two hymenopteran<span> parasitoids, such as </span></span><em>Phanerotoma</em> sp. (Braconidae: Cheloninae) and <span><em>Apanteles</em><em> taragamae</em></span> Viereck (Braconidae: Microgastrinae) were associated with <em>C. obliquifasciella.</em> To best of our knowledge, this is the first description of <em>C. obliquifasciella</em> and their associated natural enemies from the Indian region.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"37 ","pages":"Article 100517"},"PeriodicalIF":3.9,"publicationDate":"2023-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49749380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a sensitive and user-friendly allele-specific PCR assay for adulteration detection of spices","authors":"Xu Zhang,&nbsp;Ruiying Wang,&nbsp;Dandan Wang,&nbsp;Hongtao Wang","doi":"10.1016/j.jarmap.2023.100518","DOIUrl":"https://doi.org/10.1016/j.jarmap.2023.100518","url":null,"abstract":"<div><p>Spice adulteration not only seriously interferes with their flavoring functions but also leads to life-threatening poisoning for consumers. To overcome the limitations of traditional methods in spice adulteration detection, a multiplex allele-specific PCR system was developed for molecular discrimination of four commonly used spices, <span><em>Foeniculum vulgare</em></span> Mill., <span><em>Zanthoxylum bungeanum</em></span> Maxim., <span><em>Illicium verum</em></span> Hook.f., and <em>Syzygium aromaticum</em><span><span><span> (L.) Merr. &amp; L.M.Perry, from their corresponding adulterants based on chloroplast SNP markers. The developed assay, eliminating the obstacles of </span>DNA sequencing<span> and false negative results, can detect 0.1% of spice adulteration down to 0.01 ng level of </span></span>genomic DNA<span> with absolute allelic specificity and favorable efficiency. Based on the results, a standard operating procedure for using multiplex allele-specific PCR for spice adulteration detection was established. Therefore, the present study provided a simple, reliable, and sensitive molecular method for adulteration detection of spices.</span></span></p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"37 ","pages":"Article 100518"},"PeriodicalIF":3.9,"publicationDate":"2023-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49749382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microsatellite markers’ based molecular divergence among the breeding lines of aromatic marigold (Tagetes minuta L.)","authors":"Ajay Kumar ,&nbsp;Hari Sharan ,&nbsp;Diksha Dhiman ,&nbsp;Rahul Dev Gautam ,&nbsp;Ramesh Chauhan ,&nbsp;Ashok Kumar ,&nbsp;Satbeer Singh ,&nbsp;Sanatsujat Singh","doi":"10.1016/j.jarmap.2023.100514","DOIUrl":"https://doi.org/10.1016/j.jarmap.2023.100514","url":null,"abstract":"<div><p>Aromatic marigold (<span><em>Tagetes minuta</em></span><span><span> L.) is an essential oil-bearing plant of industrial value. The essential oil obtained from the plant has high demand in the flavor and perfume industry. The present study investigates the molecular diversity patterns using microsatellite markers in 126 </span>breeding lines<span> representing three groups (higher, intermediate, and lower biomass) based on phenotypic characterization. These lines are being maintained through repeated selfing<span>. A total of 503 alleles with an average of 16.23 alleles per locus were detected at 31 SSR<span> loci over all the genotypes. The average most common and rare alleles were 3.35 and 12.87, respectively, over all the studied loci. The high polymorphic information content (0.88) and genetic diversity (0.89) indicate high allelic diversity among the genotypes studied. Ten genotype-specific markers were identified. Significant molecular variances have been observed among the groups (3 %), among individuals (72 %), and within individuals (26 %). The unweighted neighbor-joining clustering based on a simple matching dissimilarity index grouped all 126 genotypes into five major clusters. The clustering pattern primarily follows the biomass-specific distribution of populations. The principle coordinate analysis depicts considerable variations and again confirms the biomass-specific grouping of genotypes. The highly significant positive association between genotypic and phenotypic distances indicates the importance of these marker loci for future marker-assisted selection and association mapping studies.</span></span></span></span></p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"37 ","pages":"Article 100514"},"PeriodicalIF":3.9,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49749384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An appraisal of arbuscular mycorrhiza-mediated augmentation in production of secondary metabolites in medicinal plants","authors":"Sarda Devi Thokchom,&nbsp;Samta Gupta,&nbsp;Rupam Kapoor","doi":"10.1016/j.jarmap.2023.100515","DOIUrl":"10.1016/j.jarmap.2023.100515","url":null,"abstract":"<div><p><span>Medicinal plants<span> produce secondary metabolites, which have pharmaceutical efficacy in diverse </span></span>diseases<span>. Plant-derived secondary metabolites are utilized in different industrial sectors including pharmaceuticals<span>, food, cosmetics, and agrochemical. Reportedly, 80% of the global population rely on medicinal plants for primary health care<span><span><span>. The increasing commercial demand of secondary metabolites in recent years has, thus, led to the adoption of different strategies to improve secondary metabolite production in plants. Optimization of biological resources to enhance the productivity of plants is an important way to create sustainable bioeconomy for the 21st century. With a score of studies reporting the positive impact of arbuscular mycorrhizal fungi (AMF) on producing important secondary metabolites in medicinal plants, this article reviews the status quo of AMF-medicinal plant association. Furthermore, it critically discusses the influence of symbiotic association on plant primary metabolism, which ultimately regulates secondary metabolite production and accumulation. The review introduces the symbiotic association between AMF and medicinal plants, followed by a brief account of plant metabolites’ roles in establishing and maintaining the symbiosis. In continuation, the influence of AMF on different medicinally important secondary metabolites such as alkaloids, </span>flavonoids, phenolics, and </span>terpenoids are briefly discussed. Furthermore, the underlying mechanisms of AMF-mediated accumulation of secondary metabolites are discussed, including the plant’s response mechanism to AMF colonization encompassing the morphological, physiological, and molecular responses. In conclusion, the article emphasizes the prospects of mycorrhizal technology in sustainable cultivation practices of medicinal plants.</span></span></span></p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"37 ","pages":"Article 100515"},"PeriodicalIF":3.9,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49108167","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High throughput pre-breeding evaluation of Greek oregano (Origanum vulgare L. subsp. hirtum) reveals multi-purpose genotypes for different industrial uses","authors":"Eirini Sarrou ,&nbsp;Eftichia Martinidou ,&nbsp;Luisa Palmieri ,&nbsp;Ioanna Poulopoulou ,&nbsp;Foteini Trikka ,&nbsp;Domenico Masuero ,&nbsp;Gauly Matthias ,&nbsp;Ioannis Ganopoulos ,&nbsp;Paschalina Chatzopoulou ,&nbsp;Stefan Martens","doi":"10.1016/j.jarmap.2023.100516","DOIUrl":"10.1016/j.jarmap.2023.100516","url":null,"abstract":"<div><p><span><em>Origanum vulgare</em></span> L. subsp. <em>hirtum</em><span><span><span> (commonly known as Greek oregano) is a perennial species of the </span>Lamiaceae<span> family that counts numerous applications in the food and pharmaceutical industry. The exploitation of Greek oregano </span></span>germplasm<span><span><span> lies in its unique diversity in secondary metabolites<span>, which are accumulated in the aerial parts of the plant. For the industrial exploitation of this crop the starting germplasm is of high importance in terms of valorizing well characterized chemotypes with desired and stable agronomic and </span></span>phytochemical<span> traits. For this reason, a pre-breeding evaluation of twenty-one accessions collected and preserved under the same environmental conditions was performed in order to develop a descriptive database containing: a) morphometric traits, b) the genetic structure, c) the extensive metabolic fingerprint in terms of volatile composition, polyphenolic and </span></span>triterpenoid content, and d) the ‘bio-activity’ on the embryonic development of </span></span><span><em>Ascaridia galli</em></span><span> eggs for highly divergent accessions. This study revealed the existence of essential oil rich chemotypes superior in morphometric characteristics, containing high carvacrol<span> content (&gt; 75%) and traces of thymol<span> (&lt; 5%), according to the requested international standards. Apart from genotypes considered as ‘high yield essential oil-producers’ (&gt; 6%), accessions of highly productivity of ‘green’ raw materials rich in polyphenols and triterpenoids were also identified. According to their rosmarinic acid content, Greek oregano accessions were classified in three groups; 1) those with significantly high concentration (1640–2726 mg 100 g</span></span></span>⁻¹ dry weight), 2) those expressing intermediate content (1103–1200 mg 100 g⁻¹ dry weight), and 3) the ones with significantly low content (&lt; 873 mg 100 g⁻¹<span><span> dry weight). A total of 118 polymorphic alleles were identified using sequence-related amplified polymorphism (SRAPs) and simple sequence repeat (SSRs) markers, while 2 genetic clusters based on a single data matrix with SRAPs and SSRs alleles, were identified. The presence of private bands in some accessions was also observed, which can be used in future breeding programs for germplasm authentication purposes. As a result this study provides a comprehensive knowledge on metabolic and </span>genetic diversity of Greek oregano accessions native to Greece, as well as powerful tools for the selection of starting genetic materials for subsequent breeding studies to create superior cultivars. The multifaceted approach was developed to exploit the genetic resources of Greek oregano in further breeding programs for subsequent selection of genotypes with desired traits.</span></p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"37 ","pages":"Article 100516"},"PeriodicalIF":3.9,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44216912","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening and validated semi - quantification high-performance thin layer chromatography method development for lupeol, lupeol acetate, β-sitosterol, ρ-coumaric acid and proto-catechuic acid in the root extracts of Hemidesmus indicus (L.) R.Br. & Decalepis hamiltonii Wight & Arn.","authors":"Sujith Thatipelli ,&nbsp;Murugammal Shanmugam ,&nbsp;Shakila Ramachandran ,&nbsp;Gopinath Pushparathinam","doi":"10.1016/j.jarmap.2023.100510","DOIUrl":"10.1016/j.jarmap.2023.100510","url":null,"abstract":"<div><p><em>H. indicus</em><span> (L.) R. Br. roots are widely used in traditional medicine systems in India. </span><em>D. hamiltonii</em> Wight &amp; Arn. It looks like <em>H. indicus</em><span> and is substituted in the traditional herbal market. Five marker compounds, namely lupeol<span>, lupeol acetate (LA), β-sitosterol (BS), ρ-coumaric acid (PC) and protocatechuic acid (PCA) were quantified, and the method was validated. Separation of lupeol (R</span></span>_f max 0.48), LA (R_f max 0.75) and BS (R_f<span><span> max 0.36) was achieved using hexane: </span>ethyl acetate (8:2, </span><em>v/v</em>) and scanned at λ520 nm after derivatization with vanillin–sulphuric acid reagent (VSR). For PC (R_f 0.59) and PCA (R_f<span> 0.47), the separation was performed using toluene: ethyl acetate: formic acid (7:5:0.5, </span><em>v/v/v</em>) and scanned at λ254 nm. The validation parameters include linearity, accuracy, precision, the limit of detection and quantification (LOD and LOQ), repeatability, specificity and recovery as per ICH guidelines. Lupeol, LA and BS were found to be present in both plants; PC was present in <em>H. indicus,</em> and PCA was present in <em>D. hamiltonii</em>. The calibration plots were linear in the range (<em>μ</em>g/band) of 5–15 for lupeol; 20–45 for LA; 1–5 for PC; 5–20 for BS, and PCA. The LOD were 0.0181, 0.023, 0.080, 0.0021 and 0.0019 (<em>μ</em>g/band) for lupeol, LA, BS, PC and PCA, respectively, concerning area, correlation coefficients (r²) were 0.9980, 0.9988, 0.9958, 0.9998 and 0.9938 for lupeol, LA, BS, PC and PCA respectively. The validated high-performance thin layer chromatography (HPTLC) method provided an excellent linear relationship for all the quantified analytes; hence it may be used for quantitative estimation of the above markers to assess the quality of <em>H. indicus</em> and <em>D. hamiltonii</em> or herbal formulations containing them.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"36 ","pages":"Article 100510"},"PeriodicalIF":3.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47375909","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sustainable agriculture of tropical spices: arbuscular mycorrhizal fungi as an ecotechnological tool − A critical review","authors":"Rositta Varghese ,&nbsp;J.G. Ray","doi":"10.1016/j.jarmap.2023.100507","DOIUrl":"10.1016/j.jarmap.2023.100507","url":null,"abstract":"&lt;div&gt;&lt;p&gt;&lt;span&gt;A thorough critical analysis of the existing literature on arbuscular mycorrhizal fungi&lt;span&gt; (AMF) in spices provides significant findings. Although the International Standard Organisation (ISO) has listed about 109 plant products as spices and fifty or more spice crops are commonly used worldwide, AMF studies in spices currently are limited. However, a high diversity of forty-one species of AMF is reported from the rhizosphere of ten spices included in this study. Among the AMF species, &lt;/span&gt;&lt;/span&gt;&lt;span&gt;&lt;em&gt;Glomus&lt;/em&gt;&lt;/span&gt; spp. (some of which are now known by new names), followed by &lt;span&gt;&lt;em&gt;Acaulospora&lt;/em&gt;&lt;/span&gt;&lt;span&gt;&lt;span&gt;&lt;span&gt;&lt;span&gt; spp., dominate spice fields. In spices, the AMF provides nutritional benefits such as increased accumulation of P and other nutrients, especially K, N, Fe, Zn, and Mn. Thereby, AMF boosts the photosynthetic activity of spices by enabling them to have more chlorophyll and carotenoid pigments. Thus, AMF directly contributes to diverse plant growth characteristics, including fruiting quality and seed yield in spices. AMF also stimulates &lt;/span&gt;secondary metabolite&lt;span&gt; production in spice crops by directly enhancing biomass production&lt;span&gt; or indirectly activating the secondary metabolite synthesis pathway. The synthesis of terpenoids in spices is connected with increased absorption of nutrients such as phosphorus. Thus, AMF contributes indirectly to the quality of spices. Experimental studies state that AMF is crucial in certain spice crops' &lt;/span&gt;&lt;/span&gt;&lt;/span&gt;biofortification&lt;span&gt;, especially selenium (Se). The extra nutritional benefits of AMF in the cultivation of spice crops include overcoming diverse environmental stresses, pests, and diseases&lt;span&gt;. Therefore, AMF is a well-known inevitable biological component in spice fields, essential to sustainable cultivation of spices and improved spice quality. However, among the AMF known in spices, identification up to the species level is not available in many cases. Knowledge of AMF concerning soil types, seasons, and other environmental variables is limited. Moreover, the ecology of AMF in spice fields, especially optimization of factors concerning the effectiveness of specific AMF in specific spices, is significant to its application as a natural tool for sustaining soil fertility and crop productivity. Therefore, intensive research on AMF diversity of more spice crops over diverse soil and agroclimatic regions in different seasons has become essential worldwide. In addition, AMF activity concerning the root morphology&lt;span&gt; and architecture in specific spices is also significant. Such studies need to be complemented with intensive experimental studies for optimization in variables (plant, fungal, and other environmental factors) in the judicious application of AMF in cultivating spices. A comprehensive review of research on all such aspects of AMF applications in spice crops has yet to appear in the literature. Since the global deman","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"36 ","pages":"Article 100507"},"PeriodicalIF":3.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46598221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vegetative propagation of Berberis asiatica Roxb. Ex DC., Ginkgo biloba L., Rauvolfia serpentina (L.) Benth ex Kurz, and Rhododendron arboreum Sm. through stem cuttings","authors":"L.S. Lodhiyal ,&nbsp;Neelu Lodhiyal ,&nbsp;Riya Gupta ,&nbsp;Sushma Tamta ,&nbsp;Falak Siddiqui ,&nbsp;Harsh Kumar Chauhan","doi":"10.1016/j.jarmap.2023.100509","DOIUrl":"10.1016/j.jarmap.2023.100509","url":null,"abstract":"<div><p><span><span><span>The population of medicinal plants such as </span>Berberis<span><span> asiatica, Ginkgo biloba, </span>Rauvolfia serpentina<span>, and Rhododendron arboreum is declining from their natural habitats due to high commercial exploitation, medicinal value, slow-growing nature, and climate change. The present study was carried to assess the effects of </span></span></span>IAA<span>, IBA, and Rootex PD.01 (commercial 1000 mg kg</span></span>⁻¹<span> of indole-3-butyric acid formulation) on survival and growth performance of stem cuttings so as to develop the vegetative propagation<span> protocols for B. asiatica, G. biloba, R. serpentina, and R. arboreum. Four hundred eighty cuttings (120 cuttings of each species) with 0.5–1 cm diameter and 10 cm length were sterilized with 1 g L</span></span>⁻¹<span> bavistin and treated with 250 mg L</span>⁻¹ concentration of IAA (indole-3-acetic acid), IBA (indole-3-butyric acid) and Rootex PD.01 (1000 mg kg-1 of IBA) respectively. The survival was highest in G. biloba (90 %) followed by R. serpentina (83.3 %), B. asiatica (80 %) and R. arboreum (66.6 %). Stem cuttings treated with Rootex PD.01 exhibited maximum root length in species i.e. B. asiatica (5.2 cm), G. biloba (8.3 cm), R. serpentina (8.4 cm) and R. arboreum (6.7 cm) respectively. Other growth parameters such as shoot height, shoot diameter, number of leaves per node, number of leaves per plant, and root number were also maximum in stem cuttings treated with Rootex PD.01. This study concluded Rootex PD.01 for the propagation of these species thereby ensuring inexpensive and efficient protocols for mass-scale cultivation.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"36 ","pages":"Article 100509"},"PeriodicalIF":3.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42096409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integration of multiple linear regression, principal component analysis, and hierarchical cluster analysis for optimizing dried fingerroot (Boesenbergia rotunda) extraction process","authors":"Traiphop Phahom ,&nbsp;Jun'ichi Mano","doi":"10.1016/j.jarmap.2023.100511","DOIUrl":"10.1016/j.jarmap.2023.100511","url":null,"abstract":"<div><p>Fingerroot (<em>Boesenbergia rotunda</em><span><span>) is a medicinal plant<span>. Recently, it was reported to have the highest potent anti-SARS-CoV-2 activity among 122 Thai medicinal plants, owing to its phenolic compounds. In this study, we aimed to optimize the conditions for extracting phenolics and their functional properties from dried fingerroot. To design the extraction conditions, fifteen treatments<span> were obtained from a combination of three independent variables (temperature, time, and methanol content in acetone) using a Box-Behnken design. The extraction conditions were evaluated based on total phenolic content (TPC), ferric ion reducing antioxidant power (FRAP), and acrolein scavenging ability (ACSA). These values were fitted to a quadratic polynomial model utilizing </span></span></span>multiple linear regressions (MLR). Principal component analysis (PCA), together with hierarchical cluster analysis (HCA), was then used to select the optimal conditions. The predictive models well described the TPC and ACSA. Employing the optimized conditions, i.e., 45 °C, 60 min, and 75% methanol, resulted in the extract having 2.98 mg GAE g</span>_dw⁻¹, 2.02 mg TE g_dw⁻¹, and 0.156 nmol s⁻¹g_dw⁻¹ for TPC, FRAP, and ACSA, respectively. These results were 3.5-, 4.1-, and 2.5-fold higher than the lowest values predicted by the developed models for TPC, FRAP, and ACSA, respectively. The extraction conditions for TPC, FRAP, and ACSA from dried fingerroot were successfully optimized. The combined technique (MLR+PCA+HCA) proposed in this study yielded results comparable to those obtained using conventional techniques. Therefore, it can be used as an alternative optimization method.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"36 ","pages":"Article 100511"},"PeriodicalIF":3.9,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45337696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}