Journal of Applied Research on Medicinal and Aromatic Plants最新文献

{"title":"Species discrimination of Fritillaria Bulbus using PCR-CRISPR/Cas12a-based nucleic acid detection","authors":"Dongfan Yang,&nbsp;Yanchao Yang,&nbsp;Wanjun Lin,&nbsp;Meina Shi,&nbsp;Zifeng Huang,&nbsp;Xuening Zhang,&nbsp;Wenzhe Ma","doi":"10.1016/j.jarmap.2024.100589","DOIUrl":"10.1016/j.jarmap.2024.100589","url":null,"abstract":"<div><div><em>Fritillaria</em> Bulbus (FB) is a traditional Chinese herbal medicine known for its efficacy in relieving coughs and alleviating asthma. It is frequently used in conjunction with other <em>Fritillaria</em> species due to their highly similar morphological characteristics. Given their considerable medicinal and economic value, convenient and accurate methods for identifying these herbal medicines are essential. This study introduces a nucleic acid detection method that integrates PCR amplification of the target region of nuclear ribosomal DNA with CRISPR/Cas12a mediated trans-cleavage of a fluorescent reporter. This method distinguishes between the two most commercially valuable species of FB, specifically <em>Fritillaria Cirrhosa</em> Bulbus (FCB) and <em>Fritillaria Ussuriensis</em> Bulbus (FUB). A conserved fragment of nuclear ribosomal DNA was chosen as the target sequence for designing crRNAs specific to each species. Both crRNAs exhibit high sensitivity in detecting amplified genes, with a detection limit of 3.0 ng/μl. No cross-reactivity was detected with non-target species, indicating high specificity<em>.</em> The practicality of this method was validated through the analysis of standard medicinal materials and real-world samples. Compared to DNA barcoding, this method exhibited superior capability in detecting mixed samples, thereby establishing a benchmark for the application of CRISPR/Cas-based nucleic acid detection in verifying the authenticity of traditional Chinese medicinal materials.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"43 ","pages":"Article 100589"},"PeriodicalIF":3.8,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142530017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient in-vitro regeneration protocol for large-scale propagation of Aloe vera (L.) Burm.f.","authors":"Sarfraz Ahmad ,&nbsp;Mohd. Tariq ,&nbsp;Saniya ,&nbsp;Vikas Belwal ,&nbsp;Maksud Hasan Shah ,&nbsp;Pankaj Kumar ,&nbsp;Meenakshi Sharma ,&nbsp;Chittaranjan Kole ,&nbsp;Mukul Jain ,&nbsp;Prashant Kaushik","doi":"10.1016/j.jarmap.2024.100588","DOIUrl":"10.1016/j.jarmap.2024.100588","url":null,"abstract":"<div><div><em>Aloe vera</em> a medicinal shrub, is used in pharmaceuticals and cosmetics due to its therapeutic properties, but its male sterility and self-incompatibility make seed-based reproduction challenging. The current investigation provides a detailed account of a refined <em>in-vitro</em> regeneration process, discussing direct and indirect organogenesis techniques. Shoot multiplication, callus formation, organogenesis in callus culture, and rooting were all evaluated in the Murashige and Skoog (MS) medium employing several plant growth regulators (PGRs). BAP (N6-benzyl amino purine) alone at 3.5 mg/l was the most effective treatment for rapid shoot multiplication and the healthiest shoot quality in direct shoot proliferation. It was shown that a combination of 2.0 mg/l Kn (Kinetin) and 1.0 mg/l NAA (1-naphthalene acetic acid) was most responsive for regeneration in callus culture, whereas 2.5 mg/l 2,4-D (2,4-dichloro phenoxy acetic acid) produced the most profuse regenerative callusing. In addition, plantlets treated with 1.5 mg/l IBA (indole-3-butyric acid) <em>in-vitro</em> produced the highest and longest roots, contributing to a 94 % survival rate during the subsequent acclimatization process. Moreover, regeneration efficiency <em>via</em> direct and indirect channels is also briefly discussed. This research has the potential to enhance the efficiency, precise application of appropriate PGRs needed for the mass production of <em>Aloe vera</em> at various stages of <em>in-vitro</em> culture.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"43 ","pages":"Article 100588"},"PeriodicalIF":3.8,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142530018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of microbiomes and mycotoxins revealing the importance in the standardized storage of Aesculus wilsonii Rehd fruit","authors":"Jing Mo ,&nbsp;Shiqi Yu ,&nbsp;Zhaohua Shi ,&nbsp;Hailan Zhu ,&nbsp;Lichun Ye ,&nbsp;Yuxin Zhou ,&nbsp;Jing Nie ,&nbsp;Bo Wang","doi":"10.1016/j.jarmap.2024.100586","DOIUrl":"10.1016/j.jarmap.2024.100586","url":null,"abstract":"<div><div><em>Aesculus wilsonii</em> Rehd, a medicinal food homology plant, whose fruit are used to treat diseases such as edema and exudation of soft tissue. Nonetheless, the presence of microbial and mycotoxin contaminants in <em>A. wilsonii</em> fruit is still unclear<em>.</em> In this study, high-throughput sequencing and liquid chromatograph tandem-mass spectrometry were conducted to determine the diversity of fungal microbiome and mycotoxin contents in moldy <em>A. wilsonii</em> fruit. The prevalent fungi in <em>A. wilsonii</em> fruit included <em>Fusarium, Colletotrichum, Dictyostelium,</em> and <em>Aspergillus</em>. Furthermore, mycotoxin tests showed that all samples tested positive for ochratoxin A, with deoxynivalenol detected in 72.4 % of samples, zearalenone in 24.1 %, and T-2 toxin in 62.1 %, revealing these mycotoxins constituted the primary contaminants. Moreover, to monitor the mycotoxin-producing fungi, multiplex ligation-dependent probe amplification (MLPA) targeting key genes in toxin biosynthetic pathway of <em>Fusarium</em> and <em>Aspergillus</em> was established. and it can detect fungi with DNA content less than 20 ng. In conclusion, this study reported the microbiome diversity and the prevalence of fungi contamination in <em>A. wilsonii</em> fruit, while also established a rapid method for detecting toxigenic fungi. This work focused on analyzing and detecting microbiomes and mycotoxins in <em>A. wilsonii</em> fruit, providing a reference for the study of potentially harmful microorganisms in the storage of medicinal products.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"43 ","pages":"Article 100586"},"PeriodicalIF":3.8,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142438331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fungal communities in three root herbs: Insights and implications","authors":"Yanan Xu ,&nbsp;Chune Fan ,&nbsp;Huilin Yuan,&nbsp;Yufeng Li,&nbsp;Jianping Han,&nbsp;Meihua Yang,&nbsp;Xiaohui Pang","doi":"10.1016/j.jarmap.2024.100587","DOIUrl":"10.1016/j.jarmap.2024.100587","url":null,"abstract":"<div><div>The roots of Astragali Radix (AM), Dioscoreae Rhizome (DR), and Codonopsis Radix (CR) are frequently used in classical tonic formulations and dietary supplements. Given the extended exposure of rhizomes to complex biological environments, it is necessary to investigate the fungal composition of their surface. In this study, the fungal communities in the three root herbs were analyzed by DNA metabarcoding, and an extensive comparison of the fungal diversity at each taxonomic level was carried out. Furthermore, we examined the effects of species, collection site, and processing method on the fungal community. In Astragali Radix and Codonopsis Radix samples, <em>Cladosporium</em> was the predominant genus, with relative abundances of 1.98 %-76.81 % and 1.69 %-85.59 %, respectively. <em>Aspergillus</em> (0.08 %–99.92 %) was the prevailing genus in Dioscoreae Rhizome samples. Meanwhile, a total of 12 potential toxigenic fungi were identified, including <em>Aspergillus restrictus</em>, <em>Fusarium oxysporum</em>, and <em>Penicillium citrinum</em>. Moreover, the variations in fungal diversity and community composition from different collection sites and processing approaches were observed. Linear discriminant analysis effect size indicated significant differences in the relative abundance of genera among the three root herbs. <em>Gibberella</em> and <em>Mucor</em> genera were significantly enriched in Astragali Radix samples, while <em>Yarrowia</em> and <em>Cladosporium</em> genera exhibited significant enrichment in Dioscoreae Rhizome and Codonopsis Radix samples, respectively (<em>p</em>≤0.001). This study presents novel insights into the fungal profiles of three root herbs, thereby providing references for their safe utilization and quality improvement.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"43 ","pages":"Article 100587"},"PeriodicalIF":3.8,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142442359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasonic-assisted extraction of polyphenolics from Schrebera swietenioides Roxb.: An optimization using response surface methodology","authors":"Asha Humbal ,&nbsp;Laxman Singh ,&nbsp;Bhawana Pathak","doi":"10.1016/j.jarmap.2024.100585","DOIUrl":"10.1016/j.jarmap.2024.100585","url":null,"abstract":"<div><div><em>Schrebera swietenioides</em> Roxb is an important medicinal plant used for the treatment of various diseases by the indigenous communities. However, this plant is still underexplored in the modern scientific era. Specifically, the leaves of the plant are reported to contain various medicinal properties such as antioxidant, antimicrobial, anti-inflammatory, and antipyretic activity. Herewith, the present work demonstrates and optimizes the extraction process usingan ultrasonic system and comopared the results with conventional extraction methods. Optimization was performed by combining first and second-order polynomial designs at different levels using Plackett-Burman and Central-Composite designs. A total of 31 experiments were conducted at different levels, including ultrasonic power (10–40 %), solvent-to-sample ratio (20–50 mL/g), solvent concentration (50–80 %), and HCl normality (0.2–1 N). The findings of the study suggest a significant (p&lt;0.05) influence of extraction parameters on the extraction yield of the response variables, including total phenol (TPC), total flavonoid (TFC), total tannin (TTC), and the antioxidants activity. Further, the predicted values demonstrated good agreement with the experimental values, exhibiting a high (R²&gt;95%) coefficient of determination and a non-significant lack of fit. At the optimized condition, the experimental values of TPC, TFC, TTC and antioxidants (DPPH, ABTS, FRAP) activity were found near the model predicted values. The HPLC-PDA analysis identified ten polyphenolic compounds, among which rutin hydrate (12.991±0.003 mg/g DW), followed by phloridzin (6.660±0.001 mg/g DW), and chlorogenic acid (3.380±0.008 mg/g DW) were the most prominent compounds. Furthermore, the optimized extract showed effective antibacterial activity against <em>Bacillus subtillis</em>, <em>Escherichia coli</em>, <em>Pseudomonas aeruginosa</em>, and <em>Staphylococcus aureus</em> species. In conclusion, the obtained results indicate that the proposed optimization strategy can be utilized to enhance the yield of polyphenolics from the target species with possibility of its application in therapeutics values.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"43 ","pages":"Article 100585"},"PeriodicalIF":3.8,"publicationDate":"2024-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142424688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dormancy-break and germination of buried milk thistle (Silybum marianum (L.) Gaertn.) seeds","authors":"Marzieh Moradi ,&nbsp;Farshid Ghaderi-Far ,&nbsp;Hamid Reza Sadeghipour ,&nbsp;Asieh Siahmarguee ,&nbsp;Benjamin Torabi ,&nbsp;Carol C. Baskin ,&nbsp;Elias Soltani","doi":"10.1016/j.jarmap.2024.100581","DOIUrl":"10.1016/j.jarmap.2024.100581","url":null,"abstract":"<div><div>Milk thistle (<em>Silybum marianum</em> (L.) Gaertn.) is a medicinal/oil crop plant that produces dormant seeds at maturity. Seeds are released before harvest into the soil seed bank and hence can cause weed control problems in agricultural ecosystems. So far, information on dynamic changes of dormancy state, viability and persistence of milk thistle seeds in the soil seed bank is lacking. Accordingly, in this study the effects of ex-situ (dry in laboratory) and in-situ (buried in soil) storage conditions on dormancy-break and germination were investigated. Freshly harvested (FH) seeds were tested for germination at constant temperature ranging from 5 to 35 °C under both light/dark and dark conditions with or without GA₃. Also, FH seeds were buried in June 2019 at depths of 5, 10, and 20 cm in a field and exhumed after 2–12 months of burial and assessed for germination. FH seeds did not germinate at any test conditions, but GA₃ and after-ripening in dry storage increased germination, indicating that seeds have non-deep physiological dormancy (PD). With increased dry after-ripening, the ceiling temperature for germination (30 °C) did not change but the base temperature for germination decreased to 5 °C, i.e, seeds exhibited type 2 non-deep PD. Increased seed burial depth increased the length of the in situ after ripening period necessary for complete dormancy alleviation. Furthermore, persistence of seeds in the soil was affected by burial depth. Seeds buried at 5 cm had a transient seed bank and those at 10 and 20 cm a persistent seed bank.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"43 ","pages":"Article 100581"},"PeriodicalIF":3.8,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142424687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a method for the simultaneous estimation of terpinene-4-ol and 1,8-cineole in Melaleuca alternifolia (Maiden & Betche) Cheel and Rosmarinus officinalis L. oil using HPTLC coupled with a QbD approach","authors":"Monika S. Sonawane ,&nbsp;Ummesalama Rampurawala ,&nbsp;Sanjay P. Anantwar","doi":"10.1016/j.jarmap.2024.100584","DOIUrl":"10.1016/j.jarmap.2024.100584","url":null,"abstract":"<div><div>This paper comprehends a Quality by design (QbD) approach for development of a simple and precise HPTLC (High performance thin layer chromatography) method for simultaneous estimation of the phytoconstituents terpinen-4-ol and 1,8-cineole found in <em>Melaleuca alternifolia</em> (tea tree oil) and <em>Rosmarinus officinalis</em> (rosemary oil) respectively. The method was optimized by Design expert software using response surface methodology. The chromatographic condition optimized by using software was, mobile phase as hexane: ethyl acetate: glacial acetic acid in the ratio of 8.5:1.5:0.47 % v/v, stationary phase as TLC plates precoated with silica gel 60 F₂₅₄ at ambient temperature conditions and saturation time 20 min at 366 nm. Optimized conditions were verified and validated as per ICH Q2 (R1) (International council for harmonization of technical requirements of pharmaceuticals for human use) guidelines. The method developed was validated for the parameters such as specificity, linearity, DL, QL, accuracy and precision. This method was then applied for simultaneous estimation of terpinen-4-ol and 1,8-cineole in <em>Melaleuca alternifolia</em> (tea Tree oil) and <em>Rosmarinus officinalis</em> (rosemary oil) respectively.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"43 ","pages":"Article 100584"},"PeriodicalIF":3.8,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142424686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Agronomical and biotechnological strategies for modulating biosynthesis of steviol glycosides of Stevia rebaudiana Bertoni","authors":"Mitali Mahajan ,&nbsp;P. Naveen ,&nbsp;Probir Kumar Pal","doi":"10.1016/j.jarmap.2024.100580","DOIUrl":"10.1016/j.jarmap.2024.100580","url":null,"abstract":"<div><div>Steviol glycosides (SGs), the diterpenoids group of plant secondary metabolites, possess phytopharmaceutical properties. Steviol glycosides are principally obtained from the stevia (<em>Stevia rebaudiana</em>) plant, which belongs to the Asteraceae family. Nowadays SGs are mainly used in the food and beverage industries as a non-caloric natural sweetener, which is about 300 times sweeter than sucrose. Among the known SGs, stevioside, and rebaudioside-A are the most abundant in the stevia leaf. Recent studies have shown that agronomic and biotechnological factors principally govern the accumulation of SGs in stevia plants. Therefore, to meet the burgeoning demand for SGs, efforts have been made to modulate the biosynthesis of these bioactive compounds in stevia using different agronomic and biotechnological strategies. In this review, we lucidly discussed agronomic and biotechnological approaches that stimulate and enhance the biosynthesis of SGs in stevia. Detailed mechanistic information could support us in making a plan for the production of SGs through stevia cultivation. We also outline some promising areas for future research that would inspire studies aiming to understand the consequence of interaction effects among the environmental and agronomic factors for the biosynthesis of SGs.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"43 ","pages":"Article 100580"},"PeriodicalIF":3.8,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142530020","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a rapid quality assessment technique for Radix Paeoniae Alba (Paeonia lactiflora Pall.) using near-infrared spectroscopy and chemometrics analysis","authors":"Liu Yang ,&nbsp;Zhewen Zhang ,&nbsp;Xianjie Kang ,&nbsp;Yingjie Fang ,&nbsp;Pei Ye ,&nbsp;Weifeng Du","doi":"10.1016/j.jarmap.2024.100582","DOIUrl":"10.1016/j.jarmap.2024.100582","url":null,"abstract":"<div><div><em>Radix Paeoniae</em> Alba (RPA) was subjected to a quick quality evaluation procedure using near-infrared (NIR) spectroscopy and chemometrics. The orthogonal partial least squares discrimination analysis (OPLS-DA) method was applied to the spectrum analysis based on SIMCA software, and a qualitative discriminant model was constructed to differentiate between the origin of RPA. Additionally, the NIR spectroscopy quantitative analysis models of gallic acid, methyl gallate, oxypaeoniflorin, catechin, albiflorin, paeoniflorin, 1,2,3,4,6-O-pentagalloylglucose, and benzoylpaeoniflorin were established by partial least squares method, with the content of components determined by HPLC serving as the reference value. To select the optimal spectroscopy pretreatment technique, the correlation coefficient R, root mean square error of calibration, root mean square error of prediction, and performance index were employed as assessment indices. The variable importance projection map was created using the OPLS-DA method to maximize the detection spectral band. The optimal number of factors was then determined using cross-validation, using the anticipated residual error sum of squares and the root mean square error of cross-validation as indicators. Ultimately, a quantitative model of the NIR spectrum was established using partial least squares with the spectral area of 9997.17 ∼ 8612.53 cm⁻¹. Standard normal variation, second derivative, and no smoothing were used as pretreatments for the spectrum. The correlation coefficients of the eight components were all over 0.99, according to the model. Rapid, stable, dependable, and free of chemical reagent usage are the characteristics of the qualitative and quantitative models created in this work, which can be applied to the quick assessment of RPA's quality.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"43 ","pages":"Article 100582"},"PeriodicalIF":3.8,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142553186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High light intensity improves yield of specialized metabolites in medicinal cannabis (Cannabis sativa L.), resulting from both higher inflorescence mass and concentrations of metabolites","authors":"Wannida Sae-Tang ,&nbsp;Ep Heuvelink ,&nbsp;Céline C.S. Nicole ,&nbsp;Elias Kaiser ,&nbsp;Kjell Sneeuw ,&nbsp;Mexximiliaan M.S.F. Holweg ,&nbsp;Sabrina Carvalho ,&nbsp;Iris F. Kappers ,&nbsp;Leo F.M. Marcelis","doi":"10.1016/j.jarmap.2024.100583","DOIUrl":"10.1016/j.jarmap.2024.100583","url":null,"abstract":"<div><div>Medicinal cannabis (<em>Cannabis sativa</em> L.) contains various plant specialized metabolites, such as cannabinoids and terpenoids. These metabolites are mainly accumulated in inflorescences and are the primary focus of cultivation. Medicinal cannabis is often cultivated in indoor farming with electrical light, which allows for light intensities to optimize quantity and quality of production. Although it is known that an increase in light intensity results in increased inflorescence yield in cannabis, its impact on specialized metabolites remains unclear. We aim to quantify the effects of light intensity on the yield of specialized metabolites, and to elucidate which plant traits explain these effects, using a yield component analysis. Hereto, we conducted an experiment in a climate-controlled chamber using broad-band white LEDs at three light intensities: 600, 800, and 1000 µmol m⁻² s⁻¹ photosynthetic photon flux density (PPFD), applied during the generative phase (8 weeks with a 12-h photoperiod) in cannabis cv. ‘Critical CBD’. Yield of specialized metabolites, including cannabinoids and terpenoids, strongly increased with increasing PPFD due to increases in both their concentrations and inflorescence yield. The inflorescence yield showed a proportional increase with PPFD, resulting in a constant light use efficiency. The higher inflorescence dry mass was mainly caused by an increase in total plant dry matter production, and to a lesser extent a larger fraction of dry mass partitioned to the inflorescences. Leaf photosynthesis was higher for plants grown at higher PPFD. This study shows that cannabis can use very high light intensity, resulting in high yield of specialized metabolites due to high inflorescence mass and metabolite concentrations.</div></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"43 ","pages":"Article 100583"},"PeriodicalIF":3.8,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142424685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}