Journal of Applied Research on Medicinal and Aromatic Plants最新文献

{"title":"Harnessing the power of artificial neural networks methodology and multi-objective optimization for enhanced yield and bioactivity of plants polyphenolic compounds","authors":"Yousra Touami ,&nbsp;Rafik Marir","doi":"10.1016/j.jarmap.2024.100551","DOIUrl":"10.1016/j.jarmap.2024.100551","url":null,"abstract":"<div><p>The extraction of polyphenolic compounds from plants is crucial in the industrial production of functional nutraceuticals, but traditional methods often yield low and variable results. In this research, an innovative strategy for optimizing polyphenol extraction from two plants <em>Cistus creticus L.</em> and <em>Ephedra alata</em> subsp. <em>alenda</em> (Stapf) Trab., known for their rich composition in polyphenols and their bioactivities, using Ultrasound-Assisted Extraction in conjunction with artificial neural networks (ANNs) and multi-objective optimization is presented. ANNs were trained to model the intricate relationships among UAE parameters, including solvent concentration, temperature, and time, and the outcomes, encompassing polyphenol yield and bioactivity. Multi-objective optimization techniques were subsequently applied to identify extraction conditions that maximize both yield and bioactivity simultaneously. Results validate the accuracy of the ANNs model in predicting polyphenol yields and the significant enhancement in extraction efficiency and bioactivity achieved through multi-objective optimization. The extracts prepared in the optimal conditions have demonstrated superior antioxidant activities, compared to the non-optimized extracts, with the smallest values of IC₅₀ of 242,378 µg/mL, and 146,736 µg/mL for the plants <em>Ephedra alata</em> subsp <em>alenda</em> (Stapf) Trab. and <em>Cistus creticus</em> L. respectively. This study introduces a promising approach for elevating the extraction of plant-derived polyphenols, augmenting their bioactivity with ANNs and multi-objective optimization. In light of the obtained results, it is recommended that further research explore the scalability and applicability of the presented innovative strategy in larger-scale industrial settings. Considering the demonstrated success in optimizing polyphenol extraction from <em>Cistus creticus</em> L. and <em>Ephedra alata</em> subsp. <em>alenda</em> (Stapf) Trab., extending the application of Ultrasound-Assisted Extraction, coupled with artificial neural networks (ANNs) and multi-objective optimization, to other plant species could offer valuable insights. Additionally, investigating the economic feasibility and environmental impact of implementing this strategy on an industrial scale would contribute to its practical viability.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"41 ","pages":"Article 100551"},"PeriodicalIF":3.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141046751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Studies on viability and efficacy of various invigoration techniques to improve germination parameters of Bergenia ciliata (Haw.) Sternb.","authors":"Kajal Mog Chaudhuri ,&nbsp;Manoj Kanti Debnath ,&nbsp;Puspendu Dutta","doi":"10.1016/j.jarmap.2024.100556","DOIUrl":"10.1016/j.jarmap.2024.100556","url":null,"abstract":"<div><p><em>Bergenia ciliata,</em> a small perennial herb with lithotriptic properties, belongs to the family Saxifragaceae. The rhizome, which is also the most significant commercial part of this plant, is typically used for propagation since the seeds are very tiny with poor viability and germination capacity. Seed invigoration is a well-recognized and effective technique to improve seed germination potential, ensure establishment, and enhance yield under varying environments. Thus, the present investigation aimed to study the seed viability as well as the effect of various invigoration techniques on germination parameters and seedling vigor of <em>B. ciliata</em>. Seeds were primed with 14 distinct conventional treatments varied either with concentrations or duration of exposures. Standardized method was followed for seed germination test, and the emerged seedlings were allowed to grow until day 15 when the various seedling parameters were analyzed. Seed soaking in TZ solution followed by bleaching with 4% NaOCl for 20 min recorded 88.5–95.8% viability. Results also indicated that germination and seedling growth parameters were greater under all priming treatments as compared to control. The maximum germination rate (93.75%) was observed under GA₃@25 ppm. Total seedling length, dry weight and SVI were found to be higher under pre-sowing seed treatment with GA₃@25 ppm in comparison with control or other treatments. Better activation of ‘pre-germinative metabolisms’ in primed seeds might result in improved germination and other indices. Moreover, the PCA and cluster analysis revealed that various pre-sowing treatments particularly GA₃ would be recommended for improved seed germination of <em>B. ciliata</em>.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"41 ","pages":"Article 100556"},"PeriodicalIF":3.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141130043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species authentication of Panax ginseng C.A. Mey. and ginseng extracts using mitochondrial nad2 intron 4 region","authors":"Yuxue Pang,&nbsp;Xiaoran Tian,&nbsp;Dandan Wang,&nbsp;Hongtao Wang","doi":"10.1016/j.jarmap.2024.100554","DOIUrl":"10.1016/j.jarmap.2024.100554","url":null,"abstract":"<div><p>Species authentication of <em>Panax ginseng</em> C.A. Mey. and its commercial products is vital for ensuring its therapeutic efficacy and food safety. To address the limitations of previous DNA methods in identifying ginseng products with severe DNA degradation, a multiplex PCR assay was established based on the single nucleotide polymorphism (SNP) markers exploited from the mitochondrial <em>nad</em>2 intron 4 region. This assay, which requires neither expensive equipment nor sequence analysis of PCR products, can detect 0.1% adulteration of <em>P. quinquefolius</em> or <em>P. notoginseng</em> down to a 0.001 ng level of template DNA. The developed assay proved effective in authenticating the botanical origin of commercial ginseng extracts without generating false-negative results under traditional PCR conditions. Therefore, the present study provides a valuable technical reference for regulating adulteration in commercial ginseng products, and the methodology can be applied in species authentication of other herbal products.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"41 ","pages":"Article 100554"},"PeriodicalIF":3.9,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141130329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Production, marketing and consumption of herbal drugs in Estonia","authors":"Janne Sepp ,&nbsp;Olga Shepeleva ,&nbsp;Anneli Kask ,&nbsp;Gerda Nelis ,&nbsp;Anna-Paulina Huttunen ,&nbsp;Karin Kogermann ,&nbsp;Jyrki Heinämäki ,&nbsp;Oleh Koshovyi ,&nbsp;Ain Raal","doi":"10.1016/j.jarmap.2024.100544","DOIUrl":"https://doi.org/10.1016/j.jarmap.2024.100544","url":null,"abstract":"<div><p>The aim of the present study was to map and gain knowledge on the current status of the production, marketing and consumption of herbal drugs in Estonia. To accomplish this goal, we collected data from herbal farms, wholesalers, health stores and consumers. Our study revealed that the Estonian farms produce annually about 14,000 kg of herbal drugs, which are mainly marketed <em>via</em> community pharmacies. The most widely used herbs for medicinal purposes were <em>Chamomillae flos</em>, <em>Lini semen</em>, <em>Rosae fructus, Carum carvi semen</em>, and <em>Cetraria islandica</em>. According to the wholesale data total 650,000 packages (20,000 kg) of herbal drugs were purchased annually from community pharmacies. 80 % of the herbal drugs were of Estonian origin and 20 % imported. The sales ratio between herbal blends and single herbal drugs was 1:3. Our calculations show that one Estonian inhabitant uses an average of 10 g of herbal drugs, purchased from community pharmacy, every year. Our study also revealed that herbal blends were primarily used for general well-being and mood (27 %), for the treatment of common cold (17 %), for energy and organism stimulation (12 %), for calming, stress and sleep (11 %), and for mild gastrointestinal disorders (7 %). The most popular herbal drugs supplied by grocery and health stores were <em>Chamomillae flos, Menthae folium, Tiliae flos, Foeniculi fructus</em> and <em>Calendulae flos.</em> Perhaps surprisingly, total 1.1 million packages of herbal drugs were purchased annually from community pharmacies (representing 57 % of all packages) and from grocery and health stores (43 %). This means that one Estonian inhabitant uses an average of 20 cups of herbal tea altogether every year. Our survey about the consumption habits of herbal drugs showed that total 88 % of the respondents used herbal drugs occasionally, and approximately 31 % of them even on the weekly basis for common cold, gastrointestinal disorders and various mild inflammations. In conclusion, traditional herbal drugs have still an important role in the self-medication of common diseases in Estonia, thus supporting the contemporary pharmaceutical care.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"40 ","pages":"Article 100544"},"PeriodicalIF":3.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140641019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring GGE biplot, stability index, correlation, and clustering analysis for growth and seed yield-related traits in celery (Apium graveolens L.)","authors":"Mandeep Singh ,&nbsp;Usha Nara ,&nbsp;Kirandeep Kaur ,&nbsp;Vivek Pandey ,&nbsp;Dharminder Pathak ,&nbsp;Manjeet Kaur Sangha ,&nbsp;Pritpal Singh","doi":"10.1016/j.jarmap.2024.100546","DOIUrl":"10.1016/j.jarmap.2024.100546","url":null,"abstract":"<div><p>The effect of genotype by environment interaction is one of the limiting factor for successfully identifying superior genotypes in plant breeding programs. The study aimed to identify celery genotypes with high and stable yields across different environments by assessing genotype-environment (GE) interactions, stability indices, and associations between agronomic parameters. Twenty celery genotypes were sown in randomized complete block design field trials at three locations in Punjab, India, <em>viz</em>., Ludhiana, Amritsar, and Gurdaspur during 2018–19, 2019–20, and 2020–21. Phenotypic data for thirteen agronomic parameters were collected for GE interactions study. The investigation mainly focused on the significance of GGE biplots in explaining GE interactions based on seed yield-related traits. Results revealed significant differences in genotypes, locations, years, and interactions in the studied traits. The WAASB (weighted average of absolute sScores) stability index estimation was highlighted to identify superior genotypes with maximum seed yield and high stability. The investigation specified the suitability of MTSI (multi-trait stability index), which considers multiple traits through genotype-ideotype distance, for assessing stability. Seed yield showed a positive and significant correlation with primary branches and umbels per plant that would be effective in simultaneous selection for multiple traits. Based on MTSI, three genotypes, PAU1, PAU9, and PAU10, were identified as stable and high-yielding across all three tested locations. The investigation suggests that efforts should focus on identifying location-specific genotypes using multi-location and multi-year data for varietal releases rather than emphasizing the overall performance of genotypes.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"40 ","pages":"Article 100546"},"PeriodicalIF":3.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140775901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of different extraction solvents on bioactive phenolics and antioxidant potential of Illicium griffithii fruit","authors":"Om Prakash Arya ,&nbsp;Indra Dutt Bhatt ,&nbsp;Kaustubha Mohanty","doi":"10.1016/j.jarmap.2024.100547","DOIUrl":"https://doi.org/10.1016/j.jarmap.2024.100547","url":null,"abstract":"<div><p>The fruits of <em>Illicium griffithii</em> are of medicinal and economic importance. In this study, five solvents viz. methanol, ethanol, acetone, ethyl acetate and water at 20–100% concentration analyzed for phenolic content (TPC and TFC) and antioxidant activities (DPPH and ABTS) of <em>I. griffithii</em> fruit. TPC was highest (40.06±0.48 mg GAE/g dw) in 80% MeOH, and TFC was highest (6.58±0.12 mg QE/g dw) in 100% EtOAc extracts. The antioxidant activities were higher in extract(s) with 100% of each solvent (93.35–94.27% inhibition) and highest in 100% EtOAc extracts. A total of 7 phenolic compounds, viz. gallic acid, catechin, chlorogenic acid, p-hydroxybenzoic acid, vanillic acid, ferulic acid, and phloridzin, were identified in different solvent extracts. 100% MeOH extracted the highest content of gallic acid (2.54 mg/g) and catechin (2.59 mg/g). The ATR-FTIR spectra showed the presence of aliphatic, aromatic, and phenolic groups. The TGA analysis exposed volatile matter, cellulose, hemicellulose, and lignin content. The FESEM analysis also revealed the morphological changes in treated samples. The extraction efficacy of phenolic compounds was better in extracts with higher concentration of solvents (60% −100%). The fruit is a rich source of phenolics and antioxidants; thus, it can provide multiple industrial benefits.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"40 ","pages":"Article 100547"},"PeriodicalIF":3.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140880106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the effects of metallic toxicity on the seed germination and initial growth of Lavandula stoechas L. and Myrtus communis L.: Towards promoting the dissemination of aromatic plants in contaminated lands","authors":"Mohammed Dadach","doi":"10.1016/j.jarmap.2024.100545","DOIUrl":"https://doi.org/10.1016/j.jarmap.2024.100545","url":null,"abstract":"<div><p>The high accumulation of heavy metals (HMs) in soils is becoming a major ecological concern worldwide that negatively impacts all living organisms. Therefore, sustainable solutions are urgently needed to withstand these challenging circumstances and to maintain natural ecosystem functionality. Among the relevant solutions is to select well-adapted plant species and embed them in revegetation operations for degraded areas. In this study, the effects of different concentrations (0, 200, 400, and 600 mg L^–1) of cadmium (Cd), chromium (Cr), nickel (Ni), and lead (Pd) on the seed germination capacity and initial growth performance of two valuable aromatic plants (<em>Lavandula stoechas</em> L. and <em>Myrtus communis</em> L.) were tested. The germination of seeds was greatest in the control treatment (0 mg L^–1), while amplifying HM stress restricted germination. The results obtained showed that seeds of <em>L. stoechas</em> were unable to germinate in the presence of high doses of Cd (400 and 600 mg L^–1) and Ni (600 mg mg L^–1), and subsequently featuring increasing tolerance thresholds in the following order: Pb &gt; Cr &gt; Ni &gt; Cd. Regardless of the HM used, <em>M. communis</em> seeds germinated at all concentrations, and germination increased in the following pattern of HM resistance: Cd &gt; Ni &gt; Cr &gt; Pb. Seedlings of <em>L. stoechas</em> displayed better resistance to Ni and Pb, while <em>M. communis</em> seedlings were most strongly affected by Ni. Overall, the hypocotyl was typically more susceptible to different metal elements than the radicle. The ability of seeds to effectively emerge in a broad range of HM solutions indicates that both species can further establish and propagate in contaminated soils.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"40 ","pages":"Article 100545"},"PeriodicalIF":3.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140646848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qualitative changes of Blue eryngo (Eryngium planum L.) phytochemicals as affected by different drying methods","authors":"Abdolbaset Mahmoudi,&nbsp;Mohammad-Taghi Ebadi,&nbsp;Mahdi Ayyari","doi":"10.1016/j.jarmap.2024.100543","DOIUrl":"https://doi.org/10.1016/j.jarmap.2024.100543","url":null,"abstract":"<div><p><em>Eryngium planum</em> L. known as Blue eryngo is used as fresh and dried ornamental plant along with medicinal applications. It is used in folk medicine in Europe as Eryngii plani herba and Eryngii plani radix. The purpose of this study was to investigate the effect of different natural (shade (SHD<span>¹</span>) and sun (SD<span>²</span>) drying) and artificial drying methods (oven (OD<span>³</span>), vacuum oven (VOD<span>⁴</span>), infrared (ID<span>⁵</span>), freeze (FD<span>⁶</span>) and microwave (MD<span>⁷</span>) drying) on the essential oil (EO) content and composition, total phenolic and flavonoid content and also antioxidant capacity (DPPH) of Blue eryngo. The energy consumption of drying in SHD, SD, FD, OD at 40, 50 and 60 ℃, VOD at 40, 50 and 60 ℃, ID at 125, 132 and 220 W/cm² and MD at 100, 200, 300, 400 and 500 W were also calculated. The isolation of EOs was done by hydro-distillation method and their components were identified by gas chromatography (GC) and gas chromatography/mass spectrometry (GC-MS). The results disclosed that the highest (0.19 g/100 g D.M.) and lowest (0.08 g/100 g D.M.) essential oil content related to VOD at 60 ℃ and fresh plant, respectively. The highest (30.8 %) and the lowest (14.7 %) β-Elemene (as a main compound) was obtained by OD at 60 ℃ and SD, respectively. SHD needed the highest drying time (70.7 h) whereas the lowest one (0.2 h) related to MD at 500 W. The highest (44.8 kW.h) and the lowest (0.1 kW.h) energy consumption were related to FD and MD at 500 W, respectively. Different drying treatments showed different total phenolic and flavonoid content, and the highest total phenolic content related to ID at 220 W/cm² with 66.62 mg GAE/g dry weight of extract. The OD at 50 ℃ showed the highest total flavonoid content with 6.5 mg QE/g dry weight of extract and the highest antioxidant capacity related to OD 50 ℃ with IC50 192.66 µg/ml. In conclusion, the best drying methods based on energy consumption, time of drying, EO content and composition for <em>E. planum</em> are MD 500 W, OD 60 ℃ and VOD 50 and 60 ℃.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"40 ","pages":"Article 100543"},"PeriodicalIF":3.9,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140646805","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Establishment of HPLC method and optimization of ultrasonic-assisted extraction process for two lignan active substances in Asarum heterotropoides","authors":"Yushu Sui ,&nbsp;Liben Pan ,&nbsp;Nan Yang ,&nbsp;Xiaorui Guo ,&nbsp;Zhonghua Tang","doi":"10.1016/j.jarmap.2024.100542","DOIUrl":"10.1016/j.jarmap.2024.100542","url":null,"abstract":"<div><p>In the present study, developed a dependable HPLC technique to quantify asarinin and sesamin in <em>Asarum heterotropoides</em> Fr. Schmidt var. <em>mandshuricum</em> (Maxim.) Kitag. The optimal conditions for the ultrasonic-assisted extraction were determined by conducting single-factor experiment, Plackett-Burman design experiment, and Box-Behnken response surface method. Based on actual operation factors and analysis, the optimal extraction parameters were methanol volume fraction of 80% (v/v), liquid-to-material ratio of 10:1 (mL/g), ultrasonic time of 40 min, ultrasonic temperature of 51°C, and ultrasonic power of 300 W. When subjected to these conditions, the average asarinin and sesamin content were found to be 13.40 mg/g and 2.39 mg/g, respectively, with a relative error of 1.04% and 3.46%, which was consistent with the theoretical value. This study can offer a theoretical reference for the extraction of asarinin and sesamin in <em>Asarum</em>.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"40 ","pages":"Article 100542"},"PeriodicalIF":3.9,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140182051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ajania fastigiata in Zhetysu Alatau: Distribution, morphological characterization, phytochemical profiles, and optimization of extraction of bioactive constituents","authors":"Aknur Taldybay ,&nbsp;Doktorkhan Aidarbayeva ,&nbsp;Ahmet Aksoy ,&nbsp;Gulnara Sitpayeva ,&nbsp;Aizhamal Baiseitova ,&nbsp;Janar Jenis","doi":"10.1016/j.jarmap.2024.100540","DOIUrl":"10.1016/j.jarmap.2024.100540","url":null,"abstract":"<div><p>Despite the wide medicinal use of <em>Ajania fastigiata</em> (C. Winkl.) Poljakov. in folk medicine, most <em>Ajania</em> species from Central Asia have not been explored comprehensively. Resource data revealed that <em>A. fastigiata</em> growing in the Zhetysu Alatau of Kazakhstan in three gorges among the Terekty river valley, Maliy Baskan, Sarkan, and the Karasyryk gorge at altitudes of 1183–1510 m. Anatomical and morphological studies were carried out on the vegetative organs of plants and results can be noted that <em>A. fastigiata</em> is typical for xerophytic plants growing in conditions of lack of moisture. Phytochemical investigation of the main bioactive constituents, together with the plant quality standardization were determined by the methods of State Pharmacopeia. Eleven macro-, and microelements of the <em>A. fastigiata</em> were identified with analysis of multi-element atomic emission spectral. Moreover, <em>A. fastigiata</em> demonstrated notable effectiveness against enzymes related diabetes and bacterial infections (α-glucosidase, PTP1B, BNA) and encouraging antioxidant properties. It is concluded that <em>A. fastigiata</em> is widely distributed and has potential ethnobotanical use with strong phytochemical composition which pharmacological active composition extraction method was determined by optimization model. The ideal parameters were identified as follows: a temperature of 50 °C, a time of 120 minutes, and a sonication power of 30 kHz for both dichloromethane (DCM) and ethyl acetate (EtOAc) extracts of <em>A. fastigiata</em>. Under these optimized conditions, the DPPH-RSA values for the DCM and EtOAc extracts were determined to be 41.84% and 83.08%, respectively, and matched the anticipated results.</p></div>","PeriodicalId":15136,"journal":{"name":"Journal of Applied Research on Medicinal and Aromatic Plants","volume":"40 ","pages":"Article 100540"},"PeriodicalIF":3.9,"publicationDate":"2024-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2214786124000135/pdfft?md5=3b651d4bb1ff2132650498adf6c6f8f4&pid=1-s2.0-S2214786124000135-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140182019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}