Molecular authentication of Paeonia species for paeonia radix production using plastid and nuclear DNA markers

IF 3.8 2区 农林科学 Q1 PLANT SCIENCES
Jiseok Kim , Jong-Soo Kang , Hyun-Seung Park , Jae-Hyeon Jeon , Jee Young Park , Eunbi Yeo , Jung Hwa Kang , Seung Hyun Kim , Do Won Jeong , Young-Sik Kim , Hocheol Kim , Woojong Jang , Goya Choi , Byeong Cheol Moon , Tae-Jin Yang
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引用次数: 0

Abstract

Paeonia lactiflora is a perennial herbaceous plant popular in oriental medicine and as an ornamental plant. Two related species, P. veitchii and P. obovata, share several medicinal uses with P. lactiflora. To develop a molecular authentication system for these three Paeonia species, which are used to produce the Chinese herbal medicine Paeonia Radix, we generated super-barcode information. This included complete plastid genomes (plastomes) and 45S nuclear ribosomal DNA (45S nrDNA) from two accessions of P. lactiflora and one accession each of P. veitchii and P. obovata, using whole-genome sequencing data. The four plastomes range from 152,519 to 152,731 base pairs (bp) in length and have the same gene content and order. The 45S nrDNA transcriptional units range from 5879 to 6315 bp in length and show 33 SNPs among the three species. Phylogenetic analyses based on plastome and 45S nrDNA sequences clearly identified each species as a monophyletic group. Inter- and intraspecific polymorphisms were discovered among these three Paeonia species and Paeonia suffruticosa. Four molecular markers were developed to discriminate between P. lactiflora, P. veitchii, P. obovata, and P. suffruticosa, the main species used to produce Paeonia Radix in Korea and China. These markers successfully authenticated the species when applied to multiple herbal samples collected from various origins.
利用质体和核 DNA 标记对生产芍药的芍药品种进行分子鉴定
芍药(Paeonia lactiflora)是一种多年生草本植物,在东方医学中很受欢迎,也可作为观赏植物。两个相关物种 P. veitchii 和 P. obovata 与乳花芍药有多种药用价值。为了开发这三个芍药物种的分子鉴定系统,我们生成了超级条形码信息。通过全基因组测序数据,我们获得了乳花芍药(P. lactiflora)的两个品系以及芍药(P. veitchii)和芍药(P. obovata)各一个品系的完整质粒基因组(质粒)和 45S 核核糖体 DNA(45S nrDNA)。四个质粒的长度从 152,519 碱基对(bp)到 152,731 碱基对(bp)不等,基因含量和排列顺序相同。45S nrDNA转录单元长度为 5879 至 6315 bp,在三个物种中出现了 33 个 SNP。基于质体和 45S nrDNA 序列的系统进化分析清楚地将每个物种确定为一个单系群。在这三个芍药物种和芍药suffruticosa之间发现了种间和种内多态性。研究人员开发了四种分子标记,用于区分韩国和中国用于生产芍药的主要品种 P. lactiflora、P. veitchii、P. obovata 和 P. suffruticosa。将这些标记应用于从不同产地采集的多个药材样本时,成功地鉴定了物种。
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来源期刊
Journal of Applied Research on Medicinal and Aromatic Plants
Journal of Applied Research on Medicinal and Aromatic Plants Pharmacology, Toxicology and Pharmaceutics-Drug Discovery
CiteScore
6.40
自引率
7.70%
发文量
80
审稿时长
41 days
期刊介绍: JARMAP is a peer reviewed and multidisciplinary communication platform, covering all aspects of the raw material supply chain of medicinal and aromatic plants. JARMAP aims to improve production of tailor made commodities by addressing the various requirements of manufacturers of herbal medicines, herbal teas, seasoning herbs, food and feed supplements and cosmetics. JARMAP covers research on genetic resources, breeding, wild-collection, domestication, propagation, cultivation, phytopathology and plant protection, mechanization, conservation, processing, quality assurance, analytics and economics. JARMAP publishes reviews, original research articles and short communications related to research.
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