Journal of Basic Microbiology最新文献

{"title":"Early Transcriptome of Pseudomonas aeruginosa PAO1 Infected With vB_Pae_QDWS, a Short-Latent Lytic Phage.","authors":"Yinfeng Wang, Biqian Wei, Guanhua Xuan, Linhui Qin, Hong Lin, Jingxue Wang","doi":"10.1002/jobm.70105","DOIUrl":"https://doi.org/10.1002/jobm.70105","url":null,"abstract":"<p><p>Phage adsorption, genome injection, and immediate activation of host genes are critical steps that determine whether phages can complete their infection cycle. Elucidating the early molecular dynamic network of phage-host interactions is essential for understanding the dynamic balance between phage invasion and bacterial defense. In this study, Pseudomonas aeruginosa PAO1 was used as a model to investigate the early transcriptional responses during infection by lytic phage vB_Pae_QDWS, which features an extremely short latent period. Transcriptomic analysis at 0, 3, 6, and 20 min postinfection revealed a transient expression of early phage genes (gp01-gp08, gp13). Concurrently, the host exhibited broad transcriptional reprogramming, particularly in genes related to receptors such as flagella, pili, and lipopolysaccharide (LPS) biosynthesis, as well as quorum-sensing (QS) regulators including lasI, lasR, and rhlI. Correlation analyses revealed strong associations between specific early phage genes and host QS-related genes. Notably, the early phage gene gp08 exhibited a strong negative correlation with the QS regulator lasR, implying a potential inhibitory interaction. This study highlights the critical role of early phage gene expression in the initial takeover of the host and demonstrates the host's rapid transcriptional response under phage pressure, offering new insights into phage-host interactions and phage-based control strategies.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70105"},"PeriodicalIF":2.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Antifungal Efficacy of Cordycepin Against Fusarium oxysporum f. Sp. cubense: An In Silico and In Vitro Approach.","authors":"Mansoor Showkat, Nagesha Narayanappa, Jasima Ali, Basu Sudhakar Reddy, Saima Gani, Rajesh Udavant, Kahkashan Perveen, Najla A Alshaikh, Jayanthi Barasarathi, Riyaz Sayyed","doi":"10.1002/jobm.70100","DOIUrl":"https://doi.org/10.1002/jobm.70100","url":null,"abstract":"<p><p>A destructive fungal disease called Fusarium oxysporum f. sp. cubense can reduce agricultural productivity in banana fields by up to 100%. The current work investigates cordycepin, a naturally occurring substance obtained from Cordyceps species, as a sustainable antifungal agent to address this issue. Using molecular docking and simulation tools, we examined cordycepin's interactions with three essential Foc proteins: Secreted in Xylem 13 (SIX13), Foc secreted protein 9 (Fosp9), and a Cupin-type-1 domain-containing protein (FocTR4). Cordycepin demonstrated substantial binding affinities and produced stable complexes with all three targets: -9.3 kcal/mol (SIX13), -5.7 kcal/mol (Fosp9), and -8.3 kcal/mol (FocTR4), surpassing commercial fungicides such as prothioconazole and tebuconazole. Protein-protein interaction study further suggested that these targets are fundamental to broader functional networks, suggesting that cordycepin may interfere with essential physiological functions. Its antifungal action was validated by in vitro tests, where cordycepin demonstrated concentration-dependent inhibition of Foc mycelial growth, reaching almost total suppression at 1000 ppm. These results demonstrate cordycepin's promise as a cutting-edge, powerful antifungal therapy for treating Foc TR4 infections.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70100"},"PeriodicalIF":2.7,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145064716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multitrait Root Endophytic Bacillus subtilis BREB 03 Reduce Heterodera avenae Infestation in Barley.","authors":"Mukul Kumar Bhambhu, Priyanka Duggal, Anil Kumar, Shubham Saini","doi":"10.1002/jobm.70099","DOIUrl":"https://doi.org/10.1002/jobm.70099","url":null,"abstract":"<p><p>Cereal cyst nematode (Heterodera avenae) significantly hampers barley production by causing stunted growth and yield losses. This study explored the biocontrol potential of multitrait root endophytic bacteria isolated from H. avenae-infested barley roots to suppress nematode infestation. Ten endophytic bacterial strains were isolated and screened for their inhibitory effects on cyst hatching. Among them, Bacillus subtilis BREB 03 exhibited the highest hatching inhibition (48.31%), outperforming the bio-control check Azotobacter chroococcum HT-54. The promising root endophytic bacterium BREB 03, identified as a Gram-positive rod-shaped bacterium, exhibited optimal growth at 25°C and halotolerance up to 10% NaCl, along with diverse metabolic capabilities including positive catalase, oxidase, citrate utilization, and fermentation of multiple sugars. Whole-genome sequencing and phylogenomic analysis confirmed the identity of BREB 03 as B. subtilis. Further in vitro assays revealed BREB 03 capabilities for siderophore production, ammonia excretion, indole acetic acid (IAA) synthesis, and phosphorus solubilization, contributing to both nematode suppression and plant growth promotion. Seed treatment with BREB 03 significantly enhanced barley growth parameters, indicating its dual role as a biocontrol agent and biofertilizer. This study highlights B. subtilis BREB 03 as a promising alternative for sustainable management of cereal cyst nematode in barley, with potential to reduce reliance on chemical nematicides.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70099"},"PeriodicalIF":2.7,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145023389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cover: Journal of Basic Microbiology. 9/2025","authors":"","doi":"10.1002/jobm.70092","DOIUrl":"https://doi.org/10.1002/jobm.70092","url":null,"abstract":"<p><b>Cover illustration:</b></p><p>Graphical representation of direct and indirect mechanisms employed by plant growth-promoting rhizobacteria (PGPR) to support sustainable crop production. Direct mechanisms include phytohormone production, biological nitrogen fixation, phosphate solubilization, and siderophore production, while indirect mechanisms involve induction of systemic resistance, exopolysaccharide (EPS) production, osmolyte accumulation, and antimicrobial compound synthesis.</p><p>(Design: Aditya Sheershwal, Faculty of Science, Motherhood University, Roorkee, India)\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.70092","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144929527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Information: Journal of Basic Microbiology. 9/2025","authors":"","doi":"10.1002/jobm.70093","DOIUrl":"https://doi.org/10.1002/jobm.70093","url":null,"abstract":"","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 9","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.70093","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144929528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Streptomyces lothianensis sp. nov., an Antibiotic-Producing Strain Isolated From the Indian Sundarbans Mangrove Forest.","authors":"Pankaj Paul, Kaushik Biswas, Sergio de Los Santos Villalobos, Maria Edith Ortega Urquieta, Dhruba Bhattacharya, Sanmoy Karmakar, Kamalika Mazumder, Hariom Kushwaha","doi":"10.1002/jobm.70096","DOIUrl":"https://doi.org/10.1002/jobm.70096","url":null,"abstract":"<p><p>Strain KLOTTS4A1^T was isolated from the soil sediment of the mangrove forest of Lothian Island, Sundarbans, West Bengal, India. The chemotaxonomic and phenotypic characteristics of the strain indicated that the isolate belongs to the genus Streptomyces. 16S rRNA gene analysis revealed that the strain was most closely related to the strains Streptomyces indicus IH32-1^T (97.65%), Streptomyces montanus NEAU-C151^T (97.52%), Streptomyces chlorus BK125^T (97.49%) and Streptomyces mesophilus YC504^T (97.45%). After genome sequencing, the G + C content of strain KLOTTS4A1^T was 71.03 mol%. The ANI and dDDH values between the strain KLOTTS4A1^T and its closest neighbor Streptomyces indicus IH32-1^T were as follows: ANIb (84.43%), ANIm (87.59%), OrthoANI (84.57%), and GGDC values of 29.40%. All these values are significantly below the established species delineation thresholds of 70% for dDDH and 95%-96% for ANI. Cell wall composition of strain KLOTTS4A1^T showed the presence of ribose, mannose, and arabinose. The cell wall composed of LL-DAP in the peptidoglycan. Principle polar phospholipids are phosphatidylethanolamine, diphosphotidyl glycerol, and phosphatidyl inositol. Strain KLOTTS4A1^T was characterized by its predominant fatty acids, including iso-C_15:0, anteiso-C_15:0, and iso-C_16:0. Comprehensive genomic and phenotypic analyses revealed that this strain is distinct from closely related species of the genus Streptomyces. Based on these findings, strain KLOTTS4A1^T is proposed as a novel species of the genus Streptomyces, for which the name Streptomyces lothianensis sp. nov. is suggested. The type strain is KLOTTS4A1^T ( = MTCC 13769^T = NRRL B-65739^T = COL-B-01923^T).</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70096"},"PeriodicalIF":2.7,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rice Root Exudate Modulates Functional Traits of Plant-Growth-Promoting Bacteria: A Step Towards Rhizosphere Engineering","authors":"Velmurugan Thamizharasan,&nbsp;Rajasekaran Raghu,&nbsp;Maduraimuthu Djanaguiraman,&nbsp;Vaikuntavasan Paranidharan,&nbsp;Dananjeyan Balachandar","doi":"10.1002/jobm.70098","DOIUrl":"10.1002/jobm.70098","url":null,"abstract":"<div>\u0000 \u0000 <p>Rhizosphere engineering offers a promising strategy to improve crop productivity and soil health by optimizing plant-microbe interactions through targeted modulation of rhizosphere functioning. A key step in this approach is effective recruitment and functional activation of inoculated plant growth-promoting rhizobacteria (PGPR), mainly driven by root exudate-mediated signaling. This study investigates the response of five phylogenetically diversified PGPR strains, <i>Azotobacter chroococcum</i> (Ac1), <i>Azospirillum lipoferum</i> (Az204), <i>Pseudomonas chlororaphis</i> (ZSB15), <i>Bacillus altitudinis</i> (FD48), and <i>Pristia endophytica</i> (NE14) to root exudates derived from three different rice cultivars (BPT5204, Co51, and Co55) at active tillering and panicle initiation stages. Functional traits including growth, chemotaxis, biofilm formation, and cell wall-degrading enzyme activity of rhizobacteria were assessed. The results revealed strain- and cultivar-specific modulation of these traits, with NE14 and FD48 showing significant upregulation of assessed traits in response to exudates from BPT5204 and Co51. Gas chromatography-mass spectrometry profiling of root exudates confirmed compositional differences between cultivars and developmental stages, highlighting key metabolites such as hexadecanoic acid, propionic acid, octadecenoic acid methyl ester, and trans-3-hydroxycinnamic acid as potential regulators of PGPR chemotaxis, colonization, and biofilm formation. Principal component and correlation analyses identified cell wall-degrading enzymes and chemotaxis as contributors to strain variability, underscoring their role in establishing rhizosphere competence. These findings strengthen the importance of functional trait-based screening for identifying PGPR strains with high adaptability to the rhizosphere environment. By demonstrating that root exudate-mediated modulation of PGPR traits can enhance bacterial colonization and functionality, this study offers a conceptual foundation and experimental framework for PGPR-mediated rhizosphere engineering.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 10","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antagonistic Activity of Bacillus velezensis OEE1 Volatiles Against Biscogniauxia mediterranea.","authors":"Manel Cheffi, Houda Gharsallah, Rahma Mallek, Eetizez Karoui, Yaakoub Gharbi, Karim Ennouri, Mohamed Ali Triki","doi":"10.1002/jobm.70097","DOIUrl":"https://doi.org/10.1002/jobm.70097","url":null,"abstract":"<p><p>Increase in olive tree diseases caused by the wood-borne fungus, Biscogniauxia mediterranea, is a cause for concern. Given the importance of this plant in Mediterranean countries, the search for sustainable disease control measures is essential. The strain OEE1 of Bacillus velezensis was selected to evaluate the potential growth inhibitory effect of its volatile organic compounds (VOCs) against the fungus, using the sandwich plate technique, in which two bottoms of Petri dishes (one containing the bacterial culture and the other the fungal pathogen) are sealed together, allowing only VOCs to mediate the interaction with the pathogen. Results showed that the bacterial VOCs had a strong antagonistic activity against the fungus, achieving an 81.76% inhibition rate and inducing microscopic alterations in the fungal mycelium. The germination of B. mediterranea spores was completely blocked upon exposure to VOCs from B. velezensis. At the end of the experiment, extensive spore lysis was observed. The antagonistic potential was further assessed on detached olive twigs to evaluate its effect on necrosis following artificial inoculation with B. mediterranea. This preliminary study provides valuable insights into the potential use of VOCs from B. velezensis OEE1 as effective biocontrol agents against charcoal canker disease in olive trees.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70097"},"PeriodicalIF":2.7,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144955184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficient but Elusive Rhizobia Fix Nitrogen in the Wild Legumes Bituminaria bituminosa and Coronilla Viminalis.","authors":"Ana Monzón-Ramos, Sara Pérez-González, Laura Pulido-Suárez, Francisco Díaz-Peña, Ana Rodríguez-Pérez, J Alfredo Reyes-Betancort, Milagros León-Barrios","doi":"10.1002/jobm.70095","DOIUrl":"10.1002/jobm.70095","url":null,"abstract":"<p><p>In ecological restoration of degraded natural habitats, revegetation with wild native plants is a priority. Legumes play a key role in this process through nitrogen (N)-fixing symbiosis with rhizobia, obtaining N for their growth and improving soil fertility, which benefits other nonleguminous plants in the environment. This study explores the rhizobia of two wild legumes, Coronilla viminalis and Bituminaria bituminosa, found in a degraded habitat in Lanzarote (Canary Islands). We found these legumes nodulated by highly efficient N-fixing mesorhizobia harboring the symbiovars canariensis and hedysari in Mesorhizobium species distinct from those originally reported to carry these symbiovars. However, isolating these rhizobia was challenging. Despite the good plant development and the pink color of root nodules indicating effective N-fixation, these rhizobia could not be cultured in most cases. This suggests the presence of unculturable or \"fastidious\" rhizobia in the nodules, with requirements poorly mimicked in conventional rhizobial media. Additionally, the presence of fast-growing non-rhizobial endophytes in the nodules complicates the isolation of slower-growing rhizobia, which requires special care during the isolation protocol to avoid endophytes and extend incubation times. The difficulty of cultivating the rhizobia of these two wild legumes suggests that their diversity may be greater than described here.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70095"},"PeriodicalIF":2.7,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144882867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seed Biopriming From Basics to Omics: Relieving Plants From Biotic Stress Through the Microbial Way","authors":"Shriniketan Puranik,&nbsp;Jayashree Mekali,&nbsp;Kamala Jayanthi Pagadala Damodaram","doi":"10.1002/jobm.70083","DOIUrl":"10.1002/jobm.70083","url":null,"abstract":"<div>\u0000 \u0000 <p>From seed to harvest, cultivated crops face numerous biotic stresses, including insects, nematodes, and diseases, which significantly hinder their growth and vigor, resulting in substantial crop losses. In contrast to use of toxic agrochemicals, seed biopriming with microbial inoculants has emerged as an effective and eco-friendly alternative against pathogens and pests. Seed biopriming involves coating seeds with beneficial microorganisms that enhance protection and immunity against a variety of harmful pests and pathogens. These microbial agents colonize the seeds and establish themselves in the rhizosphere, reducing the impact of biotic stresses while fostering a healthier environment for plant growth. They are known to exhibit several mechanisms against pathogens and pests, like production of cell wall degrading enzymes, antibiosis, competition, induced systemic resistance, chelation of iron <i>etc</i>. Additionally, these microorganisms regulate phytohormone levels, further optimizing the physiological and metabolic characteristics of plants. This approach not only promotes robust plant growth but also enhances tolerance to deleterious bacteria, fungi, nematodes and arthropods, ensuring healthier crops. These interactions can further be well studied and expressed by using different omics approaches like metagenomics (of seed microbiome), proteomics, transcriptomics, metabolomics and differential gene expression. This review highlights the role and benefits of seed biopriming as a sustainable strategy to manage biotic stresses effectively, and the importance of omics for better understanding of complex processes during such interactions, contributing to resilient agricultural production systems and environmental sustainability.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 10","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144862204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}