Journal of Basic Microbiology最新文献_第2页

Comparative Effects of Bacillus strains applied Via Seed Biopriming and Soil Drenching Applications on the Morpho-Physiological and Transcriptional Aspects of Cotton.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-02-06 DOI: 10.1002/jobm.202400665

Tahir Mahmood, Anam Moosa, Faisal Zulfiqar, Muhammad Naveed Aslam, Hongwei Zhao, Meisam Mohammadi, Tolga İzgü, Taner Bozkurt, Temoor Ahmed, Doaa Bahaa Eldin Darwish

{"title":"Comparative Effects of Bacillus strains applied Via Seed Biopriming and Soil Drenching Applications on the Morpho-Physiological and Transcriptional Aspects of Cotton.","authors":"Tahir Mahmood, Anam Moosa, Faisal Zulfiqar, Muhammad Naveed Aslam, Hongwei Zhao, Meisam Mohammadi, Tolga İzgü, Taner Bozkurt, Temoor Ahmed, Doaa Bahaa Eldin Darwish","doi":"10.1002/jobm.202400665","DOIUrl":"https://doi.org/10.1002/jobm.202400665","url":null,"abstract":"Biofertilizers are considered as a sustainable solution for promoting the growth and productivity of crops while reducing the dependence on chemical fertilizers. There is a growing need for the sustainable agricultural solutions to lessen the reliance on chemical fertilizers; thus, evaluating Bacillus strains as biofertilizers for cotton growth promotion can support eco-friendly and economically viable crop production. Therefore, the growth promoting potential of endophytic Bacillus altitudinis strain TM22 and B. atrophaeus strain MCM61 applied as soil drenching or seed treatment, was evaluated on cotton cv. 'SS32'. In vitro, the qualitative assay both TM22 and MCM61 showed proteolytic, amylolytic, lipolytic, cellulolytic, and chitinolytic activity. TM22 and MCM61 strains also demonstrated the ability to produce siderophores, indole 3-acetic acid (IAA), and phosphate solubilization. In the pot experiment, seed biopriming with TM22 and MCM61 had better performance regarding plant growth and biomass, photosynthetic pigments, stomatal conductance, and relative leaf water contents than the soil drenching application. The gene expression analysis of growth hormones-related genes demonstrated that TM22 and MCM61 showed an upregulated expression of ARF1, ARF18, EXP6, IAA9, GIB1b, and CKX6 while ERF and ERF17 genes were downregulated. Overall, these findings suggest that seed biopriming with B. altitudinis TM22 and B. atrophaeus MCM61 is an effective method with the potential to enhance the biomass of cotton.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e2400665"},"PeriodicalIF":3.5,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Carbon-Based Nanomaterials Alter the Behavior and Gene Expression Patterns of Bacteria.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-02-02 DOI: 10.1002/jobm.202400545

Shima Afrasiabi, Alireza Partoazar, Ramin Goudarzi, Ahmad Reza Dehpour

引用次数: 0

Stress-Induced Response and Adaptation Mechanisms in Bacillus licheniformis PSKA1 Exposed With Abiotic and Antibiotic Stresses.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-02-02 DOI: 10.1002/jobm.202500016

Arihant Jayawant Kadapure, Nagarjuna Prakash Dalbanjan, Praveen Kumar S K

{"title":"Stress-Induced Response and Adaptation Mechanisms in Bacillus licheniformis PSKA1 Exposed With Abiotic and Antibiotic Stresses.","authors":"Arihant Jayawant Kadapure, Nagarjuna Prakash Dalbanjan, Praveen Kumar S K","doi":"10.1002/jobm.202500016","DOIUrl":"https://doi.org/10.1002/jobm.202500016","url":null,"abstract":"Soil ecosystems consist of diverse microbial communities with great potential for ecological and biotechnological applications. These communities encounter various abiotic stresses, which expedite the activation of transient overexpression of heat shock proteins (HSPs). In the present study, a soil bacterium was isolated and identified as Bacillus licheniformis strain PSK.A1, and its growth parameters were optimized before exposing it to heat, salt, pH, and antibiotic stress conditions. Comparative protein expression was analyzed using SDS-PAGE, protein stabilization via protein aggregation assays, and survival through single spot dilution and colony-counting methods under various stress conditions. The pre-treatment of short stress dosage showed endured overall tolerance of bacterium to lethal conditions, as evidenced by moderately enhanced total soluble intracellular protein content, better protein stabilization, comparatively over-expressed HSPs, and relatively enhanced cell survival. The findings highlighted that cells grown under optimal conditions were more susceptible to lethal environments than stressed cells, with their enhanced tolerance linked to the overexpression of 20 distinct HSPs of 17-91 kD. These insights offer the potential for developing strategies to enhance microbial resilience for various applications including bacterial bioprocessing, bio-remediation, and infectious disease management.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e2500016"},"PeriodicalIF":3.5,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Efficacy and Ultrastructural Impact of Metarhizium anisopliae and Metarhizium robertsii on Myllocerus subfasciatus.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-02-02 DOI: 10.1002/jobm.70000

Udhayakumar Monisha, Pagalahalli Sankaran Shanmugam, Marimuthu Murugan, Subramanian Jeyarani, Nandagopal Geetha, Thulasy Srinivasan, Angappan Suganthi, Rajasekaran Raghu, Kabirdoss Indhumathi, Rajendran Yamini, Murugesan Naveen, Perumal Vivekanandhan

{"title":"Efficacy and Ultrastructural Impact of Metarhizium anisopliae and Metarhizium robertsii on Myllocerus subfasciatus.","authors":"Udhayakumar Monisha, Pagalahalli Sankaran Shanmugam, Marimuthu Murugan, Subramanian Jeyarani, Nandagopal Geetha, Thulasy Srinivasan, Angappan Suganthi, Rajasekaran Raghu, Kabirdoss Indhumathi, Rajendran Yamini, Murugesan Naveen, Perumal Vivekanandhan","doi":"10.1002/jobm.70000","DOIUrl":"https://doi.org/10.1002/jobm.70000","url":null,"abstract":"The ash weevil, Myllocerus subfasciatus, is a significant insect pest that infests brinjal. Both the adults and grubs feed on the leaves and roots, respectively, leading to considerable yield loss. The subterranean habits of the larvae limit the effectiveness of insecticide applications, necessitating the implementation of integrated pest management programs that utilize entomopathogenic fungi. This investigation aimed to identify potential Metarhizium species against ash weevil larvae through scanning electron microscopy (SEM) and histopathology. The ash weevils were mass-cultured on brinjal plants under insect-proof conditions. Eleven Metarhizium sourced from the departmental repository were subjected to pathogenicity tests on second-instar ash weevil larvae, revealing that a concentration of 1 × 106 conidia/mL was optimal for SEM and histological studies. Among the 11 Metarhizium strains examined, the TNAU ENTMA TDM 8 strain produced spores measuring 5.8 µm in length and 2.4 µm in width in both potato dextrose agar (PDA) medium and larvae. SEM analysis indicated that the mycelial adherence and penetration of Metarhizium were most pronounced in the larvae 5 days post inoculation (DPI). Histopathological investigations demonstrated that the TNAU ENTMA TDM 8 strain caused degradation of fat bodies and hemocytes at 3 DPI, and complete body distortion at 7 DPI, while the untreated control exhibited no such effects. The M. robertsii strain TNAU ENTMR GYU 1 displayed slower infectivity compared to the M. anisopliae strains. The TNAU ENTMA TDM 8 strain was determined to be the most effective against M. subfasciatus larvae and can be utilized for managing ash weevil populations.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70000"},"PeriodicalIF":3.5,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Adaptive Development of Soil Bacterial Communities to Ecological Processes Caused by Mining Subsidence.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-01-31 DOI: 10.1002/jobm.70002

Yan Yu, Yuanjia Li, Jiangning Zhou, Jie Zhang, Wen Li

{"title":"Adaptive Development of Soil Bacterial Communities to Ecological Processes Caused by Mining Subsidence.","authors":"Yan Yu, Yuanjia Li, Jiangning Zhou, Jie Zhang, Wen Li","doi":"10.1002/jobm.70002","DOIUrl":"https://doi.org/10.1002/jobm.70002","url":null,"abstract":"Subsidence from coal mining is a major environmental issue, causing significant damage to soil structure. Soil microorganisms, highly sensitive to environmental changes, adapt accordingly. This study focused on four areas of the Burdai coal mine: a non-subsidence area (CK), half-yearly (HY), 1-year (OY), and 2-year (TY) subsidence areas. Using high-throughput sequencing and molecular ecological network analysis, we examined soil microbial community diversity and structure across these zones, exploring microbial community assembly and functional predictions. Results showed that compared to the control, subsidence areas experienced reduced soil water content, organic matter, available phosphorus, and alkaline nitrogen, with the lowest levels observed at 1 year. These values began to rise after 1 year, suggesting natural recovery after subsidence stabilized. Microbial communities were closely related to soil organic matter, water content, and alkaline nitrogen. At the 1-year mark, soil property changes significantly reduced microbial diversity, which then began to recover after 2 years. The microbial network during 1-year subsidence was simpler, with 102 nodes, 179 edges, and an average degree of 3.51, indicating that early subsidence was unstable, and the microbial community was still adapting. By 1 year, community structure and interactions had begun to stabilize. Stochastic processes played a key role in microbial variability during short-term subsidence.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70002"},"PeriodicalIF":3.5,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tolerance to NSAIDs in Actinobacteria From a Mexican Volcano Crater: Genomics and Bioremediation Potential.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-01-29 DOI: 10.1002/jobm.202400772

Claudia Soria-Camargo, Lorna Catalina Can-Ubando, Gauddy Lizeth Manzanares-Leal, Ayixon Sánchez-Reyes, Sonia Dávila-Ramos, Ramón Alberto Batista-García, Ninfa Ramírez-Durán

{"title":"Tolerance to NSAIDs in Actinobacteria From a Mexican Volcano Crater: Genomics and Bioremediation Potential.","authors":"Claudia Soria-Camargo, Lorna Catalina Can-Ubando, Gauddy Lizeth Manzanares-Leal, Ayixon Sánchez-Reyes, Sonia Dávila-Ramos, Ramón Alberto Batista-García, Ninfa Ramírez-Durán","doi":"10.1002/jobm.202400772","DOIUrl":"https://doi.org/10.1002/jobm.202400772","url":null,"abstract":"Non-steroidal anti-inflammatory drugs (NSAIDs) are emerging contaminants that pose significant health and environmental risks due to their persistence, including their presence in drinking water. Bioremediation, particularly through microorganisms such as actinobacteria, offers a sustainable approach to mitigate these pollutants. Actinobacteria from poly-extreme environments exhibit unique genetic and metabolic adaptations, enabling resistance to and degradation of various contaminants. This study aimed to evaluate the tolerance of actinobacteria to NSAIDs and conduct a genomic analysis of a selected strain. Actinobacteria were isolated from the crater of the Chichonal volcano [Chiapas, Mexico), resulting in 16 isolates. Among these, Micrococcus luteus P8SUE1, Micrococcus yunnanensis P9AGU1, and Kocuria rhizophila P1AGU3 demonstrated tolerance to diclofenac, ibuprofen, and paracetamol at concentrations of 1 ppm, 10 ppm, and 100 ppm, respectively. Whole-genome sequencing of M. yunnanensis P9AGU1 identified genes linked to the degradation of aromatic compounds and adaptations to extreme environmental conditions, highlighting its potential for bioremediation applications.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e2400772"},"PeriodicalIF":3.5,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metacaspases-Like Proteases of Trichomonas vaginalis: In Silico Identification and Characterization.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-01-26 DOI: 10.1002/jobm.202400786

Julio César Torres-Romero, María Elizbeth Alvarez-Sánchez, Marcos Morales-Reyna, Andrea Bellavista-Caballero, Rodrigo Arreola, Leidi C Alvarez-Sánchez, Julio Lara-Riegos

{"title":"Metacaspases-Like Proteases of Trichomonas vaginalis: In Silico Identification and Characterization.","authors":"Julio César Torres-Romero, María Elizbeth Alvarez-Sánchez, Marcos Morales-Reyna, Andrea Bellavista-Caballero, Rodrigo Arreola, Leidi C Alvarez-Sánchez, Julio Lara-Riegos","doi":"10.1002/jobm.202400786","DOIUrl":"https://doi.org/10.1002/jobm.202400786","url":null,"abstract":"Metacaspases (MCA), are cysteine-dependent proteases closely related to caspases. In protozoa, MCA plays an important role in programmed cell death (PCD). In Trichomonas vaginalis, a kind of PCD that resembles apoptosis has been described, but the activators of this mechanism have not been demonstrated. We performed a genome-wide in silico analysis in the T. vaginalis database using consensus MCA domains. A total of 15 protein annotations for MCA-like sequences were retrieved. Only 7/15 (TvMCA1-6 and TvMCA9) of the sequences were annotated as putative MCA and exhibited a similar range of amino acid length in comparison to the consensus sequences used for the query. By in silico analysis, we found that they are thermostable, hydrophilic proteins with molecular weights ranging from 27 to 33 KDa and their theoretical isoelectric points are in a 5.08-8.57 range. The phylogenetic analysis showed the similarity of conserved motifs for the predicted TvMCA proteins. 3D structure prediction by homology modeling demonstrated that TvMCA proteins show a similar conformation to crystallized MCA proteins. Taken together, our results indicate that these trichomonad proteins have conserved sequences like MCA proteins and suggest that they may be responsible for proteolytic activity during a PCD-like mechanism in this parasite.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e2400786"},"PeriodicalIF":3.5,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Special Focus: Antibiotics and Antibiotic Resistance Crisis.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-01-26 DOI: 10.1002/jobm.202500050

Erika Kothe

引用次数: 0

The Combination of α-Fe₂O₃ NP and Trichoderma sp. Improves Antifungal Activity Against Fusarium Wilt. α-Fe2O3 NP与木霉组合提高对枯萎病的抗真菌活性。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-01-19 DOI: 10.1002/jobm.202400613

Sushma Sharma, Poonam Kumari, Mamta Shandilya, Sapna Thakur, Kahkashan Perveen, Imran Sheikh, Zubair Ahmed, Riyaz Sayyed, Andrea Mastinu

{"title":"The Combination of α-Fe2O3 NP and Trichoderma sp. Improves Antifungal Activity Against Fusarium Wilt.","authors":"Sushma Sharma, Poonam Kumari, Mamta Shandilya, Sapna Thakur, Kahkashan Perveen, Imran Sheikh, Zubair Ahmed, Riyaz Sayyed, Andrea Mastinu","doi":"10.1002/jobm.202400613","DOIUrl":"https://doi.org/10.1002/jobm.202400613","url":null,"abstract":"Soil-borne plant pathogens are the most damaging pathogens responsible for severe crop damage. A conventional chemotherapy approach to these pathogens has numerous environmental issues, while biological control agents (BCAs) are less promising under field conditions. There is an immediate need to develop an integrated strategy for utilizing nanoparticles and biocontrol to manage soil-borne pathogens, such as Fusarium wilt, effectively. Simulation of BCA metabolites to nanoparticle biocontrol metabolites is considered the most effective biocontrol approach. Combining Fe2O3 nanoparticles and Trichoderma in nursery and field conditions manages pathogens and increases plant growth characteristics. The present study evaluated the commercial biocontrol strains and the use of NPFe in combination with Trichoderma harzianum to enhance the biocontrol potential of T. harzianum against soil-borne pathogens. The effectiveness of (NPFe + T. harzianum) was evaluated under in vitro conditions where combination was found most effective upto (87.63%) mycelial growth inhibition of pathogen and under field conditions lowest pooled Fusarium wilt incidence (19.54%) was recorded. Nanocomposites are beneficial for agricultural sustainability and environmental safety by upregulating the expression of genes linked to these processes, Fe NPs can activate plant defense mechanisms and increase plant resistance to pathogenic invasions. Additionally, as iron is a necessary component for plant growth and development, Fe NPs promote better nutrient uptake.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e2400613"},"PeriodicalIF":3.5,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development, Shelf Stability, and In-Vitro Evaluation of Liquid Bacterial Inoculant Acinetobacter lwoffii Strain PAU_31LN. 液体细菌接种剂lwoffii不动杆菌PAU_31LN的研制、货架稳定性及体外评价

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-01-19 DOI: 10.1002/jobm.202400617

Jagjot Kaur, Gulab Pandove, Vineet Kumar, Amal Abdullah A Sabour, Maha Alshiekheid

{"title":"Development, Shelf Stability, and In-Vitro Evaluation of Liquid Bacterial Inoculant Acinetobacter lwoffii Strain PAU_31LN.","authors":"Jagjot Kaur, Gulab Pandove, Vineet Kumar, Amal Abdullah A Sabour, Maha Alshiekheid","doi":"10.1002/jobm.202400617","DOIUrl":"https://doi.org/10.1002/jobm.202400617","url":null,"abstract":"Acinetobacter has been recognized as a versatile plant growth promoting (PGP) rhizobacteria (PGPR) that produce multiple PGP traits. The present study was conducted to formulate an efficient and stable liquid bacterial inoculant (LBI) of Acinetobacter lwoffii strain PAU_31LN. In the current investigation, total 16 endophytic bacteria were isolated from cotton leaves and evaluated for plant growth-promoting features such as production of phytohormones, mineral solubilization, siderophore production, hydrogen cyanide (HCN) production, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. The leaf endophytic bacteria designated as 31LN was found promising for all the PGP traits and it was identified as A. lwoffii strain PAU_31LN by 16S rRNA gene sequencing. For the development of LBI of A. lwoffii strain PAU_31LN, 4.5 g/L yeast extract, 5 g/L NaCl, 5 g/L peptone, and 12.5 mM food-grade trehalose was optimized as appropriate medium composition using response surface methodology (RSM) and Box-Behnken design. Further, the viability of A. lwoffii strain PAU_31LN in the optimized formulation was observed as 1.1 folds higher over the control after 180 days of storage at room temperature. Moreover, nonsignificant variation was recorded in the functional traits of 180 days old LBI of A. lwoffii strain PAU_31LN and freshly prepared LBI. The in-vitro plant growth parameters such as length and seed vigor index of 7-day-old cotton seedlings were enhanced by the seed bio-priming with LBI of A. lwoffii strain PAU_31LN over the control. The results of the present study signify the importance of endophytes and statistical methods to formulate prominent LBI.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e2400617"},"PeriodicalIF":3.5,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0