Journal of Basic Microbiology最新文献_第2页

Genome Mining and Antagonism of Stenotrophomonas geniculata MK-1, Against Peanut Foliage Fungus Diseases.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-10 DOI: 10.1002/jobm.70016

Taswar Ahsan, Mahnoor, Sulaiman Ali Alharbi, Maha A Alshiekheid, Amal Abdullah A Sabour, Ismail Khan, Mohammad Javed Ansari

{"title":"Genome Mining and Antagonism of Stenotrophomonas geniculata MK-1, Against Peanut Foliage Fungus Diseases.","authors":"Taswar Ahsan, Mahnoor, Sulaiman Ali Alharbi, Maha A Alshiekheid, Amal Abdullah A Sabour, Ismail Khan, Mohammad Javed Ansari","doi":"10.1002/jobm.70016","DOIUrl":"https://doi.org/10.1002/jobm.70016","url":null,"abstract":"Stenotrophomonas geniculata, a bacterium, has been recognized as an eco-friendly substitute for chemical fungicides in managing peanut foliar diseases, web blotch, and early leaf spot. Core genome and pan-genome analysis identified that strain MK-1 belongs to Stenotrophomonas geniculata, and nucleotide polymorphism (SNP) analysis confirmed that strain belongs to Stenotrophomonas maltophilia. The research revealed that S. geniculata MK-1 had a notable antagonistic impact on Peyronellaea arachidicola and Cercospora arachidicola and demonstrated a biocontrol efficacy of over 95% against peanut early leaf spot and web blotch disease. The nonredundant protein sequences (NR) database identified 4324 annotations related to S. geniculata, with 2682 genes similar to strain MK-1. The COG database categorized 3041 annotations into 22 functional groups, and 33 distinct metabolic pathways associated with 1851 Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations. Most genes linked with metabolism are found in S. geniculata, with 380 genes related to carbohydrate metabolism and 44 genes related to secondary metabolite biosynthesis. The Carbohydrate-Active enZYmes (CAZy) database identified 194 annotations are linked to non-ribosomal synthesis of secondary metabolites. The Pathogen-Host Interactions (PHI) database showed reduced virulence in strain MK-1, while unaffected pathogenicity protein counts were 52. The MK-1 strain can produce antifungal siderophores secondary metabolites, non-ribosomal peptide synthetase (NRPS), and siderophores.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70016"},"PeriodicalIF":3.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beneficial Tripartite Association of Pseudomonas Protegens, AM Fungi and Rice Promotes Growth Under Aerobic Conditions.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-10 DOI: 10.1002/jobm.70014

Ekta Narwal, Kannepalli Annapurna, Jairam Choudhary, Yudh Vir Singh, Vijai Pal Bhadana

{"title":"Beneficial Tripartite Association of Pseudomonas Protegens, AM Fungi and Rice Promotes Growth Under Aerobic Conditions.","authors":"Ekta Narwal, Kannepalli Annapurna, Jairam Choudhary, Yudh Vir Singh, Vijai Pal Bhadana","doi":"10.1002/jobm.70014","DOIUrl":"https://doi.org/10.1002/jobm.70014","url":null,"abstract":"The positive impact of microbial inoculants on plants is well documented. In this study, we examined two rice varieties - PB 1509 (lowland) and Satyabhama (upland) - to investigate the effects of co-inoculating AMF and Pseudomonas protegens strain Ekn 03, under aerobic conditions. Co-inoculation with Pseudomonas protegens Ekn 03 significantly enhanced root mycorrhizal colonization in both varieties, while also increasing root surface area. In dually inoculated plants, shoot fresh weight, shoot dry weight, and root dry weight were 28.5%, 87.5%, and 22.2% higher, respectively, compared to non-inoculated control plants. Dually inoculated plants showed a 31.6% increase in chlorophyll content compared to control plants. Nitrate reductase (NR) activity was 28% higher in dually inoculated plants, comparable to those treated with RDF. Additionally, a significant correlation was observed between plant biomass, NR, and glutamine synthetase (GS) activity. This tripartite association improves phosphorus and nitrogen acquisition, indicating the potential for reduced chemical input use, with P. protegens Ekn 03 identified as an effective PGPR.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70014"},"PeriodicalIF":3.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mobilome-Mediated Speciation: Genomic Insights Into Horizontal Gene Transfer in Methanosarcina.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-06 DOI: 10.1002/jobm.70013

Sivashanmuga Vadivel Saranya, R Prathiviraj, Paulchamy Chellapandi

{"title":"Mobilome-Mediated Speciation: Genomic Insights Into Horizontal Gene Transfer in Methanosarcina.","authors":"Sivashanmuga Vadivel Saranya, R Prathiviraj, Paulchamy Chellapandi","doi":"10.1002/jobm.70013","DOIUrl":"https://doi.org/10.1002/jobm.70013","url":null,"abstract":"Speciation in prokaryotes is often driven by complex genetic exchanges such as horizontal gene transfer (HGT), which facilitates genomic divergence and adaptation. In this study, we inferred the evolutionary transitions of the mobilome (plasmids, transposons, and phages) between Methanosarcina and bacteria in driving speciation within the Methanosarcina genus. By conducting evolutionary and phylogenetic analyses of Methanosarcina acetivorans, M. barkeri, M. mazei, and M. siciliae, we identified key mobilome elements acquired through HGT from distantly related bacterial species. These mobile genetic elements have shaped genomic plasticity, enabling Methanosarcina to adapt to diverse environmental niches and potentially facilitating lineage divergence. The acquisition of mobilome-associated genes involved in antibiotic resistance, DNA repair, and stress responses suggests their significant role in the ecological speciation of Methanosarcina. Overall, we hypothesized that their mobile genetic element might have been acquired from distantly related bacteria by HGT and subsequently established as new functional homologs in the present lineage. This study provides insight into how mobilome-mediated gene flow contributes to genomic divergence and speciation within microbial populations, highlighting the broader significance of mobilome in microbial evolution and speciation processes.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70013"},"PeriodicalIF":3.5,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Convergent Evolution of Coenzyme Metabolism in Methanosarcina mazei: Insights Into Primitive Life and Metabolic Adaptations.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-06 DOI: 10.1002/jobm.70015

Sivashanmuga Vadivel Saranya, Paulchamy Chellapandi

{"title":"Convergent Evolution of Coenzyme Metabolism in Methanosarcina mazei: Insights Into Primitive Life and Metabolic Adaptations.","authors":"Sivashanmuga Vadivel Saranya, Paulchamy Chellapandi","doi":"10.1002/jobm.70015","DOIUrl":"https://doi.org/10.1002/jobm.70015","url":null,"abstract":"The convergent evolution of coenzyme metabolism in methanogens provides critical insights into primitive life and metabolic adaptations. This study investigated the molecular evolution and functional dynamics of eight coenzymes and cofactors in Methanosarcina mazei, a model methanogen essential for methane production and energy conservation in anaerobic environments. Phylogenetic and genetic diversity analyses of the 706 protein sequences revealed conserved evolutionary trajectories interspersed with lineage-specific adaptations driven by gene duplication, horizontal gene transfer, and selective pressures. Key findings included the purifying selection of methanofuran (Tajima's D = -2.9589) and coenzyme A (Tajima's D = -2.8555), indicating the conservation of critical metabolic functions. The coenzyme B biosynthesis pathway showed balanced selection (Tajima's D = 2.38602), reflecting its evolutionary plasticity. Phylogenetic analyses linked coenzyme F420 biosynthetic enzymes closely to Methanosarcina horonobensis, while coenzyme F430 enzymes highlighted prokaryotic specialization distinct from their eukaryotes. Coenzyme M biosynthetic genes have demonstrated unique evolutionary connections with species across domains, such as Methanothermobacter thermautotrophicus and Gekko japonicus, emphasizing their broad adaptive significance. These evolutionary trajectories reveal how M. mazei optimized its metabolic pathways to thrive in extreme anaerobic environments, bridging ancient metabolic systems from the Last Universal Common Ancestor with contemporary ecological adaptations.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70015"},"PeriodicalIF":3.5,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fermentation Optimization of Loonamycin Production by Marine Nocardiopsis flavescens. 海洋 Nocardiopsis flavescens 生产卢安霉素的发酵优化。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-06 DOI: 10.1002/jobm.70012

Tianyu Zhou, Yuan Cao, Zhicheng Zhang, Jing Shi, Huiming Ge, Faliang An, Jiang Ye, Ruida Wang, Haizhen Wu

{"title":"Fermentation Optimization of Loonamycin Production by Marine Nocardiopsis flavescens.","authors":"Tianyu Zhou, Yuan Cao, Zhicheng Zhang, Jing Shi, Huiming Ge, Faliang An, Jiang Ye, Ruida Wang, Haizhen Wu","doi":"10.1002/jobm.70012","DOIUrl":"https://doi.org/10.1002/jobm.70012","url":null,"abstract":"The antitumor drug candidate, loonamycin, was isolated from Nocardiopsis flavescens NA01583 and exerts its antitumor activity by inhibiting topoisomerase I activity. However, the current production levels of loonamycin are insufficient for further research. To address this limitation, a liquid fermentation method was developed, optimizing both fermentation conditions and medium components. This optimization increased loonamycin production from 1 mg/mL on agar plate to 602.32 mg/mL in shake flask and ultimately 532.51 mg/mL in a 5-L bioreactor. Exogenous addition experiments revealed that sea salt and metal ions significantly inhibit loonamycin synthesis. Multi-temporal transcriptomic analyses indicated that the reduced expression levels of genes involved in the shikimate pathway and L-tryptophan biosynthesis pathway were the limiting factors for increased loonamycin yield. Overall, an effective fermentation method for rare marine actinomycetes was established, significantly enhancing loonamycin production and providing a foundation for the development of this novel antitumor prodrug.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70012"},"PeriodicalIF":3.5,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial Art Fixation on Agar Plate: MicArt Fixation for Microbiology Teaching.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-06 DOI: 10.1002/jobm.70018

Hatice Nur Halipci Topsakal, Murat Can Asarkaya, Ecem Akcan, Melih Çifçi, Rojda Karaman, Sudenaz Doğan, Elif Nur Çelik, Betül Bakir, Yasemin Günter, Okan Aydoğan

{"title":"Microbial Art Fixation on Agar Plate: MicArt Fixation for Microbiology Teaching.","authors":"Hatice Nur Halipci Topsakal, Murat Can Asarkaya, Ecem Akcan, Melih Çifçi, Rojda Karaman, Sudenaz Doğan, Elif Nur Çelik, Betül Bakir, Yasemin Günter, Okan Aydoğan","doi":"10.1002/jobm.70018","DOIUrl":"https://doi.org/10.1002/jobm.70018","url":null,"abstract":"Art based science materials engage students for learning all of the unseen microscobic creatures in easy way microbiology field. In this study we aimed to visualize bacterial and fungal species in artistic way. Inoculation of the colourful strains on agar plate as painting the agar with bacteria, provides beautiful round scenes and clear reminiscence related with the species learning for microbe world. For this purpose, we determined the different concentrations and exposure times of formaldehyde, glutaraldehyde, xylene, toluene, ethyl alcohol, epoxy, glycerin and phenol. We aimed to develop a protocol after determining the optimum storage times. As a method, we exposed all chemicals except epoxy to Petri dishes by spraying method for 1, 5, 10 min. As a result, we determined the fixative that provides the best protection is toluene. After 1 min of toluene exposure, we determined that the Petri dish with growth can be preserved for 6 months even under room conditions without disturbing the color and shape of the whole colony morphology. Here, we obtained best fixation process by using toluen, typically 1 min, depending on the species, was most promising for the preservation of art.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70018"},"PeriodicalIF":3.5,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized Production and Characterization of Auxin by Bacillus amyloliquefaciens (TSP11) for Plant Growth Promotion.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-06 DOI: 10.1002/jobm.70010

Thangarasu Suganya Devi, Karuppiah Vijay, Raja Mythili, Mohammad Ahmad Wadaan, Vasubalan Manivinoth, Woong Kim, Thangavel Kavitha

{"title":"Optimized Production and Characterization of Auxin by Bacillus amyloliquefaciens (TSP11) for Plant Growth Promotion.","authors":"Thangarasu Suganya Devi, Karuppiah Vijay, Raja Mythili, Mohammad Ahmad Wadaan, Vasubalan Manivinoth, Woong Kim, Thangavel Kavitha","doi":"10.1002/jobm.70010","DOIUrl":"https://doi.org/10.1002/jobm.70010","url":null,"abstract":"This study was aimed at isolating and recognizing the plant growth promoting rhizobacteria that secretes indole-3-acetic acid/auxin hormones. Among the 82 pure cultured bacterial morphotypes, the strain TSP11 showed high auxin production utilizing rice husk as a cheap substitute in the production medium. The minimal medium supplemented with 0.1 g/mL L-tryptophan and 1.29% rice husk produced the highest amount of auxin. Synthesized auxin was purified and quantified by chromatographic techniques. 120.08 µg/mL of synthetic medium and 135 µg/mL of agro-waste medium were the observed yields in HPLC quantification. Further GC-MS/MS and LC-MS/MS spectra exposed an existence of derived compounds such as 1H-indole, 7-methyl, indole, 3-methyl-, 1H-indole-3-carboxaldehyde (1H-indol-3-YL)-acetic acid. It was characterized by X-ray diffraction to reveal its crystallinity. Thermo gravimetric analysis showed the thermal stability of auxin over the range between 200°C and 300°C. Structural predictions of extracted auxin through HR-NMR revealed chemical shifts for Indole and acetate derivatives. The potent producer was identified as Bacillus amyloliquefaciens strain TSP11based on 16SrDNA gene sequencing. Increased seed germination percentage was observed when compared to untreated control. The research findings explore the possibility of exploiting B. amyloliquefaciens strain TSP11for bioformulations. This study represents an initial investigation into the plant growth-promoting potential of B. amyloliquefaciens strain TSP11, particularly regarding its auxin production using rice husk as a medium. We will explore the exact mechanism of auxin synthesis and its impact on various plant species in further studies.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70010"},"PeriodicalIF":3.5,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Journal of Basic Microbiology. 3/2025

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-05 DOI: 10.1002/jobm.202570006

引用次数: 0

Cover: Journal of Basic Microbiology. 3/2025

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-05 DOI: 10.1002/jobm.202570005

引用次数: 0

Harnessing Microbial Consortia for Sustainable Management of Corm Rot of Saffron.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-02-25 DOI: 10.1002/jobm.70009

Ayushi Sharma, Vishal Gupta, Gayatri Jamwal, Sushil K Gupta, Vinod Pandit, Zakir Amin, Fayaz A Mohiddin, Khalid E Hamed, Suhail Ashraf

{"title":"Harnessing Microbial Consortia for Sustainable Management of Corm Rot of Saffron.","authors":"Ayushi Sharma, Vishal Gupta, Gayatri Jamwal, Sushil K Gupta, Vinod Pandit, Zakir Amin, Fayaz A Mohiddin, Khalid E Hamed, Suhail Ashraf","doi":"10.1002/jobm.70009","DOIUrl":"https://doi.org/10.1002/jobm.70009","url":null,"abstract":"Saffron (Crocus Sativus L.) is a highly prized spice crop renowned for its significant culinary and therapeutic applications, largely attributed to its bioactive constituents such as crocin, picrocrocin, and safranal. Despite its production is severely threatened by corm rot, particularly caused by the pathogenic fungus Fusarium oxysporum, which leads to substantial yield losses in major saffron-producing regions. This review highlights the promising role of microbial consortia as an eco-friendly and sustainable strategy for effectively managing corm rot disease. The presence of diverse microbial communities can improve defense mechanisms of a plant against pathogens and support its overall growth and productivity. The mechanisms by which these microbial interactions occur, including nutrient competition, biofilm formation, and the activation of systemic resistance, contribute to their combined efficacy in inhibiting fungal pathogens. The necessity for innovative microbial strategies that harness the combined benefits of diverse microorganisms to mitigate corm rot and improve saffron cultivation sustainably. As sustainable agricultural practices increasingly gain prominence, the development and application of tailored microbial consortia gave a promising alternative to chemical fungicides, ensuring both economic viability and ecological balance in saffron production.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70009"},"PeriodicalIF":3.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0