Journal of Basic Microbiology最新文献_第10页

Transcriptional regulation of the yersiniabactin receptor fyuA gene by the ferric uptake regulator in Klebsiella pneumoniae NTUH-K2044 肺炎克雷伯氏菌 NTUH-K2044 中铁吸收调节因子对 yersiniabactin 受体 fyuA 基因的转录调控

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-04-28 DOI: 10.1002/jobm.202400001

Qian Yu, Hailin Li, Ling Du, Lifei Shen, Jiaxue Zhang, Lingyue Yuan, Huang Yao, Hong Xiao, Qunhua Bai, Yan Jia, Jingfu Qiu, Yingli Li

{"title":"Transcriptional regulation of the yersiniabactin receptor fyuA gene by the ferric uptake regulator in Klebsiella pneumoniae NTUH-K2044","authors":"Qian Yu, Hailin Li, Ling Du, Lifei Shen, Jiaxue Zhang, Lingyue Yuan, Huang Yao, Hong Xiao, Qunhua Bai, Yan Jia, Jingfu Qiu, Yingli Li","doi":"10.1002/jobm.202400001","DOIUrl":"10.1002/jobm.202400001","url":null,"abstract":"The ferric uptake regulator (Fur) is a global regulator that influences the expression of virulence genes in Klebsiella pneumoniae. Bioinformatics analysis suggests Fur may involve in iron acquisition via the identified regulatory box upstream of the yersiniabactin receptor gene fyuA. To observe the impact of the gene fyuA on the virulence of K. pneumoniae, the gene fyuA knockout strain and complementation strain were constructed and then conducted a series of phenotypic experiments including chrome azurol S (CAS) detection, crystal violet staining, and wax moth virulence experiment. To examine the regulatory relationship between Fur and the gene fyuA, green fluorescent protein (GFP) reporter gene fusion assay, real-time quantitative reverse transcription polymerase chain reaction (RT-qPCR), gel migration assay (EMSA), and DNase I footprinting assay were used to clarify the regulatory mechanism of Fur on fyuA. CAS detection revealed that the gene fyuA could affect the generation of iron carriers in K. pneumoniae. Crystal violet staining experiment showed that fyuA could positively influence biofilm formation. Wax moth virulence experiment indicated that the deletion of the fyuA could weaken bacterial virulence. GFP reporter gene fusion experiment and RT-qPCR analysis revealed that Fur negatively regulated the expression of fyuA in iron-sufficient environment. EMSA experiment demonstrated that Fur could directly bind to the promoter region of fyuA, and DNase I footprinting assay further identified the specific binding site sequences. The study showed that Fur negatively regulated the transcriptional expression of fyuA by binding to upstream of the gene promoter region, and then affected the virulence of K. pneumoniae.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"64 8","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140834622","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unique behavioral patterns of wandering colonies of Brevibacillus thermoruber on agar plates 在琼脂平板上游荡的热肠乳杆菌（Brevibacillus thermoruber）菌落的独特行为模式。

IF 3.1 4区生物学

Journal of Basic Microbiology Pub Date : 2024-04-23 DOI: 10.1002/jobm.202400091

Kazuo Sakka, Masaki Kihira, Wataru Kuhara, Akihiro Mochida, Satoru Ogawa, Tetsuya Kimura, Makiko Sakka

{"title":"Unique behavioral patterns of wandering colonies of Brevibacillus thermoruber on agar plates","authors":"Kazuo Sakka, Masaki Kihira, Wataru Kuhara, Akihiro Mochida, Satoru Ogawa, Tetsuya Kimura, Makiko Sakka","doi":"10.1002/jobm.202400091","DOIUrl":"10.1002/jobm.202400091","url":null,"abstract":"Brevibacillus thermoruber strain Nabari cells grow as widely spreading dendritic colonies on reasoner's 2A-agar (1.5%) plates at around 55°C but as small motile colonies at 37°C. Motile colonies can be divided into colonies that move in straight or curved lines over long distances (wandering colonies), and colonies that rotate at a fixed location (rotating colonies). The addition of surfactant to the agar medium greatly increased the frequency of wandering colonies and facilitated the study of such colonies. The morphology of the wandering colonies varied: circular at the tip and pointed at the back, lemon-shaped with pointed ends, crescent-shaped, bullet-shaped, fish-like, and so on. A single colony may split into multiple colonies as it moves, or multiple colonies may merge into a single colony. The most surprising aspect of the movement of wandering colonies was that when a moving colony collides with another colony, it sometimes does not make a U-turn, but instead retreats straight back, as if bouncing back. The migration mechanisms of wandering colonies are discussed based on optical microscopic observations of swimming patterns of single cells in water and scanning electron microscopy of the arrangement of bacterial cells in wandering colonies.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"64 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.202400091","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140668054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The critical role of residues Phe120 and Val161 of (2 R,3 R)‑2,3‑butanediol dehydrogenase from Neisseria gonorrhoeae as probed by molecular docking and site-directed mutagenesis 分子对接和定点突变探测淋病奈瑟菌 (2 R,3 R)-2,3 丁二醇脱氢酶残基 Phe120 和 Val161 的关键作用

IF 3.1 4区生物学

Journal of Basic Microbiology Pub Date : 2024-04-21 DOI: 10.1002/jobm.202300751

Xue Dong, Tingting Zhang, Chuanyue Gui, Shuping Fei, Haonan Xu, Jianrong Chang, Chaoqun Lian, Wanggang Tang

{"title":"The critical role of residues Phe120 and Val161 of (2 R,3 R)‑2,3‑butanediol dehydrogenase from Neisseria gonorrhoeae as probed by molecular docking and site-directed mutagenesis","authors":"Xue Dong, Tingting Zhang, Chuanyue Gui, Shuping Fei, Haonan Xu, Jianrong Chang, Chaoqun Lian, Wanggang Tang","doi":"10.1002/jobm.202300751","DOIUrl":"10.1002/jobm.202300751","url":null,"abstract":"NAD+-dependent (2 R,3 R)‑2,3‑butanediol dehydrogenase (BDH) from Neisseria gonorrhoeae (NgBDH) is a representative member of the medium-chain dehydrogenase/reductase (MDR) superfamily. To date, little information is available on the substrate binding sites and catalytic residues of BDHs from this superfamily. In this work, according to molecular docking studies, we found that conserved residues Phe120 and Val161 form strong hydrophobic interactions with both (2 R,3 R)‑2,3‑butanediol (RR-BD) and meso-2,3‑butanediol (meso-BD) and that mutations of these residues to alanine or threonine impair substrate binding. To further evaluate the roles of these two residues, Phe120 and Val161 were mutated to alanine or threonine. Kinetic analysis revealed that, relative to those of wild type, the apparent KM values of the Phe120Ala mutant for RR-BD and meso-BD increased 36- and 369-fold, respectively; the catalytic efficiencies of this mutant with RR-BD and meso-BD decreased approximately 586- and 3528-fold, respectively; and the apparent KM values of the Val161Ala mutant for RR-BD and meso-BD increased 4- and 37-fold, respectively, the catalytic efficiencies of this mutant with RR-BD and meso-BD decreased approximately 3- and 28-fold, respectively. Additionally, the Val161Thr mutant slightly decreased catalytic efficiencies (twofold with RR-BD; 7.3-fold with meso-BD) due to an increase in KM (sixfold for RR-BD; 24-fold for meso-BD) and a slight increase (2.8-fold with RR-BD; 3.3-fold with meso-BD) in kcat. These findings validate the critical roles of Phe120 and Val161 of NgBDH in substrate binding and catalysis. Overall, the current study provides a better understanding of the substrate binding and catalysis of BDHs within the MDR superfamily.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"64 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140636202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association between hepatitis C virus genotype 4 and renal cell carcinoma: Molecular and virological studies 丙型肝炎病毒基因 4 型与肾细胞癌的关系：分子和病毒学研究

IF 3.1 4区生物学

Journal of Basic Microbiology Pub Date : 2024-04-15 DOI: 10.1002/jobm.202300279

Asmaa E. Ahmed, Hassan Abol-enein, Adel A. El-Morsi, Ahmed S. El-Hefnawy, Ashraf A. Elsayed, Sherry Khater, Abdelwahab Hashem, Abdel-Rahman N. Zekri, Samia A. Haroun, Ahmed A. Shokeir, Amira Awadalla

{"title":"Association between hepatitis C virus genotype 4 and renal cell carcinoma: Molecular and virological studies","authors":"Asmaa E. Ahmed, Hassan Abol-enein, Adel A. El-Morsi, Ahmed S. El-Hefnawy, Ashraf A. Elsayed, Sherry Khater, Abdelwahab Hashem, Abdel-Rahman N. Zekri, Samia A. Haroun, Ahmed A. Shokeir, Amira Awadalla","doi":"10.1002/jobm.202300279","DOIUrl":"10.1002/jobm.202300279","url":null,"abstract":"Hepatitis C virus (HCV) is the most common infection worldwide. The correlation between HCV and renal cell carcinoma (RCC) is still mysterious. Therefore, the relationship between HCV and RCC was investigated. The study included 100 patients with RCC; 32 with HCV infection, and 68 without HCV infection. Expressions of viral proteins (NS3 and NS5A) were tested using an immune electron-microscope (IEM) and immunohistochemistry (IHC). IHC and quantitative real time-PCR investigated the presentation of human proteins TP53 and p21 genes. Transmission electron (TEM) detected viral-like particles in infected RCC tissues. The gene and protein expression of P53 was higher in HCV positive versus HCV negative patients and p21 was lower in HCV positive versus HCV negative in both tumor and normal tissue samples. Viral like particles were observed by TEM in the infected tumor and normal portion of the RCC tissues and the plasma samples. The IEM showed the depositions of NS3 and NS5A in infected renal tissues, while in noninfected samples, were not observed. The study hypothesizes that a correlation between HCV and RCC could exist through successfully detecting HCV-like particles, HCV proteins, and (p53 and p21) in RCC-infected patients.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"64 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140570260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Cover: Journal of Basic Microbiology. 4/2024 封面：基础微生物学杂志》。4/2024

IF 3.1 4区生物学

Journal of Basic Microbiology Pub Date : 2024-04-15 DOI: 10.1002/jobm.202470031

引用次数: 0

Genetic diversity and population structure of Fusarium udum in India and its correlation with pigeonpea wilt incidence 印度镰刀菌的遗传多样性和种群结构及其与鸽子豆枯萎病发病率的相关性

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-04-15 DOI: 10.1002/jobm.202300682

Beerelli Deepak Reddy, Birendra Kumar, Sangita Sahni, Gummudala Yashaswini, Somala Karthik, Morthala Shankara Sai Reddy, Rajeev Kumar, Udayan Mukherjee

{"title":"Genetic diversity and population structure of Fusarium udum in India and its correlation with pigeonpea wilt incidence","authors":"Beerelli Deepak Reddy, Birendra Kumar, Sangita Sahni, Gummudala Yashaswini, Somala Karthik, Morthala Shankara Sai Reddy, Rajeev Kumar, Udayan Mukherjee","doi":"10.1002/jobm.202300682","DOIUrl":"10.1002/jobm.202300682","url":null,"abstract":"In a study conducted in India, 50 Fusarium isolates were collected from pigeonpea growing regions and extensively examined for their cultural and morphological characteristics. These isolates exhibited significant variations in traits including growth rate, mycelial growth patterns, color, zonation, pigmentation, spore size, and septation. Subsequently, 30 isolates were chosen for pathogenicity testing on eight pigeonpea genotypes. Results showed distinct reactions, with four genotypes displaying differential responses (ICP8858, ICP8859, ICP8862, and BDN-2), while ICP9174 and ICP8863 consistently exhibited resistance and ICP2376 and BAHAR remained susceptible to wilt disease. To study the interaction between Fusarium isolates and pigeonpea host differentials (HDs), an additive main effects and multiplicative interaction analysis was conducted. The majority of disease incidence variation (75.54%) was attributed to HD effects, while Fusarium isolate effects accounted for only 1.99%. The interaction between Isolates and HDs (I × HD) contributed 21.95% to the total variation, being smaller than HD but larger than I. Based on HD reactions, isolates were classified into nine variants, showing varying distributions across pigeonpea growing states, with variants 2 and 3 being prevalent in several regions. This diversity underscores the need for location-specific wilt-resistant pigeonpea cultivars. Furthermore, genetic analysis of 23 representative isolates, through internal transcribed spacer region of ribosomal DNA and translation elongation factor 1-α gene sequencing, revealed three major clusters: Fusarium udum, Fusarium solani, and Fusarium equiseti. These findings hold potential for developing location-specific wilt-resistant pigeonpea cultivars and enhancing disease management strategies.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"64 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140570066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Issue Information: Journal of Basic Microbiology. 4/2024 期刊信息：基础微生物学杂志》。4/2024

IF 3.1 4区生物学

Journal of Basic Microbiology Pub Date : 2024-04-15 DOI: 10.1002/jobm.202470032

引用次数: 0

Salt-tolerant plant growth-promoting Pseudomonas atacamensis KSS-6 in combination with organic manure enhances rice yield, improves nutrient content and soil properties under salinity stress 耐盐植物生长促进假单胞菌 Atacamensis KSS-6 与有机肥料结合使用可提高水稻产量，改善盐分胁迫下的养分含量和土壤特性

IF 3.1 4区生物学

Journal of Basic Microbiology Pub Date : 2024-04-15 DOI: 10.1002/jobm.202300767

Naveen K. Arora, Jitendra Mishra, Pradyumna Singh, Tahmish Fatima

{"title":"Salt-tolerant plant growth-promoting Pseudomonas atacamensis KSS-6 in combination with organic manure enhances rice yield, improves nutrient content and soil properties under salinity stress","authors":"Naveen K. Arora, Jitendra Mishra, Pradyumna Singh, Tahmish Fatima","doi":"10.1002/jobm.202300767","DOIUrl":"10.1002/jobm.202300767","url":null,"abstract":"In the current study salt tolerant-plant growth-promoting rhizobacteria (ST-PGPR) Pseudomonas atacamensis KSS-6, selected on the basis of prominent plant growth-promoting (PGP) and stress tolerance properties was tested as bioinoculant to improve yield of rice grown in saline soil. The ST-PGPR KSS-6 was capable of maintaining the PGP traits up to 200 mM NaCl, however, higher salt stress conditions affected these activities. The study was designed to determine the effect of developed talc-based bioformulation using KSS-6 along with organic manure (OM) on growth and yield of paddy under saline conditions. Bioformulation broadcasting was also done to examine the effect on soil properties. It was found that the combinatorial treatment showed positive impact on growth and yield of rice under saline conditions. Co-application of KSS-6 with OM showed maximum increment in growth, chlorophyll content, plant fresh weight, and dry weight as compared to untreated control plants. Furthermore, the combinatorial treatment improved the nutrient content (P, K, Zn, Fe, Mg, and Mn) by more than 35% and enhanced the biochemical parameters such as proline, flavonoids, carbohydrates, protein, dietary fiber, and antioxidant content of rice grains by more than 32%. Soil parameters including pH and electrical conductivity (EC), moisture content, total organic carbon, OM, sodium, and chloride ions were also improved upon treatment. There was significant lowering of EC from 7.43 to 4.3 dS/m when combination of OM and bacteria were applied. These findings suggest that the application of KSS-6 in the form of bioinoculant could be a promising strategy to mitigate negative impacts of salt stress and enhance the yield and nutritional properties of rice grown in degraded and saline soil.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"64 6","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140603314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Plant growth-promoting and biocontrol traits of endophytic Bacillus licheniformis against soft rot causing Pythium myriotylum in ginger plant 内生地衣芽孢杆菌对生姜软腐病病原菌 Pythium myriotylum 的植物生长促进和生物控制特性

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-04-05 DOI: 10.1002/jobm.202300643

Khaloud Mohammed Alarjani, Mohamed S. Elshikh

{"title":"Plant growth-promoting and biocontrol traits of endophytic Bacillus licheniformis against soft rot causing Pythium myriotylum in ginger plant","authors":"Khaloud Mohammed Alarjani, Mohamed S. Elshikh","doi":"10.1002/jobm.202300643","DOIUrl":"10.1002/jobm.202300643","url":null,"abstract":"Bacterial endophytes from plants harbor diverse metabolites that play major roles in biocontrol and improve plant growth. In this study, a total of 12 endophytic bacteria were isolated from the ginger rhizome. The strain K3 was highly effective in preventing mycelia growth of Pythium myriotylum (78.5 ± 1.5% inhibition) in dual culture. The cell-free extract (2.5%) of endophyte K3 inhibited 76.3 ± 4.8% mycelia growth, and 92.4 ± 4.2% inhibition was observed at a 5% sample concentration. The secondary metabolites produced by Bacillus licheniformis K3 showed maximum activity against Pseudomonas syringae (24 ± 1 mm zone of inhibition) and Xanthomonas campestris (28 ± 3 mm zone of inhibition). The strain K3 produced 28.3 ± 1.7 IU mL−1 protease, 28.3 ± 1.7 IU mL−1 cellulase, and 2.04 ± 0.13 IU mL−1 chitinase, respectively. The ginger rhizome treated with K3 in the greenhouse registered 53.8 ± 1.4% soft rot incidence, and the streptomycin-treated pot registered 78.3 ± 1.7% disease incidence. The selected endophyte K3 improved ascorbate peroxidase (1.37 ± 0.009 µmole ASC min−1 mg−1 protein), catalase (8.7 ± 0.28 µmole min−1 mg−1 protein), and phenylalanine ammonia-lyase (26.2 ± 0.99 Umg−1) in the greenhouse. In addition, K3 treatment in the field trial improved rhizome yield (730 ± 18.4 g) after 180 days (p < 0.01). The shoot length was 46 ± 8.3 cm in K3-treated plants, and it was about 31% higher than the control treatment (p < 0.01). The lytic enzyme-producing and growth-promoting endophyte is useful in sustainable crop production through the management of biotic stress.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"64 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140570270","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Special issue: Pathogenic microorganisms 特刊：病原微生物

IF 3.1 4区生物学

Journal of Basic Microbiology Pub Date : 2024-04-02 DOI: 10.1002/jobm.202400173

Erika Kothe

引用次数: 0