Journal of Basic Microbiology最新文献_第10页

Antibiotic Resistance Dissemination and Mapping in the Environment Through Surveillance of Wastewater 通过对废水的监测，了解环境中抗生素耐药性的传播和分布情况。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-12-15 DOI: 10.1002/jobm.202400330

Neenu P. Raju, Aamir Ansari, Gandhali Patil, Mohammed Shahique Sheeraz, Sushrut Kukade, Shailendra Kumar, Atya Kapley, Asifa Qureshi

{"title":"Antibiotic Resistance Dissemination and Mapping in the Environment Through Surveillance of Wastewater","authors":"Neenu P. Raju, Aamir Ansari, Gandhali Patil, Mohammed Shahique Sheeraz, Sushrut Kukade, Shailendra Kumar, Atya Kapley, Asifa Qureshi","doi":"10.1002/jobm.202400330","DOIUrl":"10.1002/jobm.202400330","url":null,"abstract":"<div>\u0000 \u0000 Antibiotic resistance is one of the major health threat for humans, animals, and the environment, according to the World Health Organization (WHO) and the Global Antibiotic-Resistance Surveillance System (GLASS). In the last several years, wastewater/sewage has been identified as potential hotspots for the dissemination of antibiotic resistance and transfer of resistance genes. However, systematic approaches for mapping the antibiotic resistance situation in sewage are limited and underdeveloped. The present review has highlighted all possible perspectives by which the dynamics of ARBs/ARGs in the environment may be tracked, quantified and assessed spatio-temporally through surveillance of wastewater. Moreover, application of advanced methods like wastewater metagenomics for determining the community distribution of resistance at large has appeared to be promising. In addition, monitoring wastewater for antibiotic pollution at various levels, may serve as an early warning system and enable policymakers to take timely measures and build infrastructure to mitigate health crises. Thus, by understanding the alarming presence of antibiotic resistance in wastewater, effective action plans may be developed to address this global health challenge and its associated environmental risks.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 3","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142828708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Acanthamoeba Spp. Cell-Free Supernatants on Some Bacterial Pathogens Acanthamoeba Spp.无细胞上清液对某些细菌病原体的影响

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-12-12 DOI: 10.1002/jobm.202400537

Şevval Maral Özcan Aykol, Zuhal Zeybek, Yavuzhan Kayabaş, Serranur Çevikli, Nihan Berfin Keskin, Münise Hilal Kahraman, Hümeyra Çaliş

{"title":"Effect of Acanthamoeba Spp. Cell-Free Supernatants on Some Bacterial Pathogens","authors":"Şevval Maral Özcan Aykol, Zuhal Zeybek, Yavuzhan Kayabaş, Serranur Çevikli, Nihan Berfin Keskin, Münise Hilal Kahraman, Hümeyra Çaliş","doi":"10.1002/jobm.202400537","DOIUrl":"10.1002/jobm.202400537","url":null,"abstract":"<div>\u0000 \u0000 The fact that free-living amoebae of the genus Acanthamoeba can live in many different environments causes these protozoa to have different interactions with other microorganisms. Investigation of Acanthamoeba–pathogenic bacteria interaction is important for the discovery of new antibacterial agents that can be used against pathogenic bacteria. In this study, it was aimed to investigate the antibacterial effect of cell-free supernatants obtained from Acanthamoeba against some pathogenic bacteria. One standard strain (Acanthamoeba castellanii ATCC 50373) and one environmental strain (B1) of the genus Acanthamoeba were used in the study. Cell-free supernatants were obtained by centrifuging the axenic cultures (3000 rpm, 5 min) and passing through a sterile filter with a pore diameter of 0.22 µm. The antibacterial effect of cell-free supernatants against five different pathogenic bacteria (Acinetobacter baumannii, Klebsiella pneumoniae, Enterococcus faecalis, Salmonella Typhi, and Salmonella enterica) was investigated by colony counting method. As a result of the study, it was determined that the standard Acanthamoeba cell-free supernatant showed the highest antibacterial effect against E. faecalis (75.79%), while B1 cell-free supernatant showed the highest antibacterial effect against K. pneumoniae (8.5%). The content of the tested Acanthamoeba cell-free supernatants was analyzed by gas chromatography/mass spectrometry in our previous study and was also found to contain major compounds with antibacterial properties. Therefore, it is thought that the metabolites produced by Acanthamoeba can be used as an alternative to existing antimicrobial drugs in the fight against infections caused by some important pathogenic bacteria.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 3","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142817836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evolutionary Transitions of DNA Replication Origins Between Archaea and Bacteria. 古细菌和细菌DNA复制起源的进化转变。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-12-11 DOI: 10.1002/jobm.202400527

S Saranya, R Prathiviraj, P Chellapandi

{"title":"Evolutionary Transitions of DNA Replication Origins Between Archaea and Bacteria.","authors":"S Saranya, R Prathiviraj, P Chellapandi","doi":"10.1002/jobm.202400527","DOIUrl":"https://doi.org/10.1002/jobm.202400527","url":null,"abstract":"DNA replication origins play a crucial role in cellular division and are evolutionarily conserved across domains. This study investigated the evolutionary transitions of replication origins between archaea and bacteria by analyzing 2733 bacterial and 257 archaeal genomes. Our findings revealed that certain methanogens and bacteria share phylogenetic proximity, suggesting evolutionary interactions across diverse ecological systems. Evolutionary transitions in replication origins may have occurred between gut methanogens and bacteria, haloarchaea (Halogeometricum borinquense DSM 11551 and Halovivax ruber XH-70), halobacteria, and sulfur-reducing archaea. Methanosarcina barkeri (M. barkeri), Methanosaeta thermophila, and Methanococcoides burtonii (M. burtonii) were closely related to respiratory tract bacteria in humans. Methanohalobium evestigatum (M. evestigatum) is strongly linked to the animal gut pathogen Mycoplasma putrefaciens (M. putrefaciens). Several thermophilic hydrogenotrophic methanogens clustered with oral and fish pathogens. Pyrococcus furiosus (P. furiosus) was evolutionarily related to the replication origin of plant pathogens. This study sheds light on the ecological drivers of DNA replication origin evolution and their role in microbial speciation and adaptation. Our findings highlight the influence of mutualistic and parasitic relationships on these evolutionary transitions. It could have significant implications in biotechnology and medicine, such as developing novel antimicrobial strategies and understanding host-pathogen dynamics.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e2400527"},"PeriodicalIF":3.5,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142813041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A LysM Effector Mediates Adhesion and Plant Immunity Suppression in the Necrotrophic Fungus Botrytis cinerea 一种LysM效应物介导坏死性真菌灰霉病的粘附和植物免疫抑制。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-12-10 DOI: 10.1002/jobm.202400552

Mélanie Crumière, Amélie de Vallée, Christine Rascle, François-Xavier Gillet, Shamsun Nahar, Jan A. L. van Kan, Christophe Bruel, Nathalie Poussereau, Mathias Choquer

{"title":"A LysM Effector Mediates Adhesion and Plant Immunity Suppression in the Necrotrophic Fungus Botrytis cinerea","authors":"Mélanie Crumière, Amélie de Vallée, Christine Rascle, François-Xavier Gillet, Shamsun Nahar, Jan A. L. van Kan, Christophe Bruel, Nathalie Poussereau, Mathias Choquer","doi":"10.1002/jobm.202400552","DOIUrl":"10.1002/jobm.202400552","url":null,"abstract":"<div>\u0000 \u0000 LysM effectors are suppressors of chitin-triggered plant immunity in biotrophic and hemibiotrophic fungi. In necrotrophic fungi, LysM effectors might induce a mechanism to suppress host immunity during the short asymptomatic phase they establish before these fungi activate plant defenses and induce host cell death leading to necrosis. Here, we characterize a secreted LysM protein from a major necrotrophic fungus, Botrytis cinerea, called BcLysM1. Transcriptional induction of BcLysM1 gene was observed in multicellular appressoria, called infection cushions, in unicellular appressoria and in the early phase of infection on bean leaves. We confirmed that BcLysM1 protein binds chitin in the fungus cell wall and protects hyphae against degradation by external chitinases. This effector is also able to suppress the chitin-induced ROS burst in Arabidopsis thaliana, suggesting sequestration of chitooligosaccharides in apoplast during infection. Moreover, contribution of BcLysM1 in infection initiation and in adhesion to bean leaf surfaces were demonstrated. Our data show for the first time that a LysM effector can play a dual role in mycelial adhesion and suppression of chitin-triggered host immunity, both of which occur during the early asymptomatic phase of infection by necrotrophic fungi.</div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142800883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nicotinamide Mononucleotide From Bacillus licheniformis MW301654 Unravels the Nematicidal Property Against Meloidogyne incognita 地衣芽孢杆菌MW301654的烟酰胺单核苷酸揭示了其对嗜线虫的杀线虫特性。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-12-10 DOI: 10.1002/jobm.202400533

Suhail Ashraf, R. U. Krishna Nayana, Sevugapperumal Nakkeeran, Saranya Nallusamy, Nusrat Fatimah, Yousef A. Bin Jardan, Mohammad Raish

{"title":"Nicotinamide Mononucleotide From Bacillus licheniformis MW301654 Unravels the Nematicidal Property Against Meloidogyne incognita","authors":"Suhail Ashraf, R. U. Krishna Nayana, Sevugapperumal Nakkeeran, Saranya Nallusamy, Nusrat Fatimah, Yousef A. Bin Jardan, Mohammad Raish","doi":"10.1002/jobm.202400533","DOIUrl":"10.1002/jobm.202400533","url":null,"abstract":"<div>\u0000 \u0000 Research on nematode management globally highlights the use of nematicidal biomolecules and biocontrol agents. However, the availability of biomolecules to manage plant-parasitic nematodes remains limited. The discovery of microbial biomolecules offers new opportunities in this field, though they are underexplored for suppressing nematodes. This study focused on identifying biomolecules from Bacillus licheniformis (MW301654) to manage Meloidogyne incognita, a root-knot nematode infecting banana. In silico protein–ligand interactions revealed that, Nicotinamide mononucleotide, produced during the ditrophic interaction of B. licheniformis (MW301654) with Fusarium oxysporum f. sp. cubense was effective against M. incognita protein targets including cytochrome C oxidase subunit 1, calreticulin, neuropeptide G-protein coupled receptor, chorismate mutase 1, venom allergen-like proteins and β-1,4-endoglucanase than the commercially used nematicides carbofuran 3G and fluensulfone. In vitro bioassays further validated nicotinamide mononucleotide nematicidal activity. At concentrations of 93, 76, and 69 ppm, nicotinamide mononucleotide caused 50% mortality of second-stage juveniles after 24, 48, and 72 h, respectively, while 213, 132, and 101 ppm resulted in 95% mortality. Egg hatching was also significantly reduced, with only 1% hatching at 150 ppm. The study emphasized the potential of Nicotinamide mononucleotide as a novel biopesticide for the management of M. incognita infection in banana.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 3","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142800886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bottom-Up Effects of Various Plant Growth Promoting Treatments on Fitness Parameters of Hippodamia variegata 不同促生处理对花色马尾适合度参数自下而上的影响。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-12-02 DOI: 10.1002/jobm.202400486

Mozhgan Mardani-Talaee, Gadir Nouri-Ganblani, Jabraeil Razmjou, Mahdi Hassanpour, Perumal Vivekanandhan, Bahram Naseri

{"title":"Bottom-Up Effects of Various Plant Growth Promoting Treatments on Fitness Parameters of Hippodamia variegata","authors":"Mozhgan Mardani-Talaee, Gadir Nouri-Ganblani, Jabraeil Razmjou, Mahdi Hassanpour, Perumal Vivekanandhan, Bahram Naseri","doi":"10.1002/jobm.202400486","DOIUrl":"10.1002/jobm.202400486","url":null,"abstract":"<div>\u0000 \u0000 Plant growth-promoting activities using biological, chemical, and organic fertilizers are well-documented for pest insects, their impacts on predators are less commonly studied. This research investigates whether bell pepper plants treated with plant growth-promoting rhizobacteria (PGPRs), arbuscular mycorrhizal fungi (AMF), vermicompost (30%), and zinc sulfate either separately or in selected combinations affect the nutrient indices and population growth traits of the ladybug predator, Hippodamia variegata (Goeze), when fed on aphids, Myzus persicae (Sulzer). Bell pepper plants were individually treated with two PGPRs (Bacillus subtilis and Pseudomonas fluorescens), one AMF (Glomus intraradices), soil amended with 30% vermicompost (v/v), and foliar application of zinc sulfate under greenhouse conditions. Combined treatments of AMF × B. subtilis and AMF × P. fluorescens were also tested. Nutritional indices and population growth parameters of predator were reared on the treated plants infested with aphids. Results showed that the efficiency of conversion of ingested food (ECI) in predator larvae was highest on B. subtilis-treated aphids and lowest on vermicompost-treated aphids. The relative growth rate (RGR) of predator was the highest on zinc sulfate-treated aphids and lowest on vermicompost-treated aphids. Predators fed on vermicompost-treated aphids had the lowest net reproductive rate (R₀) and intrinsic rate of increase (r), while R₀ was highest for predators fed on B. subtilis-treated aphids and r was highest on P. fluorescens- and B. subtilis-treated aphids. These findings suggest that zinc sulfate and biological fertilizers involving PGPRs can enhance the ecological fitness of predators and could be effective in biocontrol-based integrated pest management of aphids.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 3","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sustainable Approach for Degradation of Low-Density Polyethylene Plastic Waste Using Ligninolytic White Rot Fungus 木质素降解白腐菌降解低密度聚乙烯垃圾的可持续方法

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-12-02 DOI: 10.1002/jobm.202400442

Mridula Chaturvedi, Navpreet Kaur, Samsul Alam, Shashi Sharma

{"title":"Sustainable Approach for Degradation of Low-Density Polyethylene Plastic Waste Using Ligninolytic White Rot Fungus","authors":"Mridula Chaturvedi, Navpreet Kaur, Samsul Alam, Shashi Sharma","doi":"10.1002/jobm.202400442","DOIUrl":"10.1002/jobm.202400442","url":null,"abstract":"<div>\u0000 \u0000 Bisphenol A (BPA), an endocrine disruptor is used in manufacturing of polycarbonate plastics for food—drink packaging. In the present study, optimized set of conditions to degrade commercial grade BPA has been used and applied in degrading shredded leached low-density polyethylene (LDPE) residues and its leachate (198 µg/L BPA) using white rot fungus Hypocrea lixii. One-at-a-time method showed maximum BPA degradation of 98.73 ± 0.02% with 190.1 ± 0.2 U/L laccase and 1913.2 ± 0.3 U/L lignin peroxidase in glucose-yeast extract-malt extract-peptone (GYMP) medium supplemented with 5% sawdust, mediators—CuSO4 (0.2 mM), veratryl alcohol (0.1 mM) and Tween 80 (0.1 mM). Three sets were prepared by dissolving these optimized nutritional components in leachates—A (only leachate), B (leached LDPE residues in leachate) and C (leached LDPE residues, sawdust in leachate). All sets showed 100% degradation in 5 days. Cracks and holes in degraded LDPE pieces was confirmed by SEM analysis and changes in functional groups by FTIR. Toxicity assay of treated leachate on soil microfauna revealed the elimination of BPA as it supported sufficient microbial growth of soil bacteria. Thus, the present process provides a sustainable solution for the management of LDPE with the possibility of using treated leachate for irrigation.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769266","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing Trichoderma asperellum: Tri-Trophic Interactions for Enhanced Black Gram Growth and Root Rot Resilience 利用曲霉木霉：三营养相互作用提高黑革生长和根腐病恢复能力。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-12-01 DOI: 10.1002/jobm.202400569

Praveen Vijayakumar, Sudha Appusami, Selva Amala Anbazhagan, Ramjegathesh Rajendran, Kavitha Shanmugam, Kahkashan Perveen, Najat A. Bukhari, Riyaz Sayyed

{"title":"Harnessing Trichoderma asperellum: Tri-Trophic Interactions for Enhanced Black Gram Growth and Root Rot Resilience","authors":"Praveen Vijayakumar, Sudha Appusami, Selva Amala Anbazhagan, Ramjegathesh Rajendran, Kavitha Shanmugam, Kahkashan Perveen, Najat A. Bukhari, Riyaz Sayyed","doi":"10.1002/jobm.202400569","DOIUrl":"10.1002/jobm.202400569","url":null,"abstract":"<div>\u0000 \u0000 Root rot caused by Macrophomina phaseolina, a common soil-borne disease in black gram, is managed with chemical fungicides, leading to toxicity and degradation of beneficial soil microbes. Existing bioagents, like talc formulation, cause leaching, clogging, and reduced productivity. The development of liquid bio-formulation via drip irrigation is crucial to mitigate biotic stress and maximize yield. This study aims to investigate the efficacy and survivability of liquid formulation of Trichoderma asperellum against root rot and its growth promotion. The results showed that Tv1 effectively inhibited M. phaseolina (66.67%), under in vitro condition. The vigor index of 4025.00 and the spore load of 1 × 108 cfu/mL were recorded from plant growth promotion and spermosphere study @ 5 mL/kg of seeds with formulation. The study found that combined application of seed treatment @ 5 mL/kg of seed and soil application @ 10 mL/L of water significantly reduced disease incidence (9.1%) against control (74.3%), with increased biomass index. There are 32 mVOCs profiled during the tritrophic interaction in roots of black gram and they were up or downregulated, viz., mollugin, pentadecanoic acid, cyclopropaneoctanoic acid, 2-octyl-, methyl ester, rhodopin, dodecanoic acid, 1,2,3-propanetriyl ester by involved in defense mechanism and biosynthetic pathways like jasmonic acid, glyconeogenic and act as acyl-CoA: acyltransferase 2 inhibitor. The results of this study confirmed that liquid formulation performs better in growth promotion, survivability on seed surface, and managing root rot of black gram compared talc-based formulation.</div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 3","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Journal of Basic Microbiology. 12/2024 期刊信息：Journal of Basic Microbiology. 12/2024

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-12-01 DOI: 10.1002/jobm.202470112

引用次数: 0

A Component of the Septation Initiation Network Complex, SepL, Participates in the Cellobiose-Responsive Expression of Cellulolytic Enzyme Genes in Aspergillus aculeatus 分离起始网络复合体SepL的一个组成部分参与了刺曲霉纤维素水解酶基因的纤维二糖响应性表达。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-12-01 DOI: 10.1002/jobm.202400266

Kazumi Sawada, Saki Kikuya, Yui Shiga, Takashi Kawaguchi, Shuji Tani

{"title":"A Component of the Septation Initiation Network Complex, SepL, Participates in the Cellobiose-Responsive Expression of Cellulolytic Enzyme Genes in Aspergillus aculeatus","authors":"Kazumi Sawada, Saki Kikuya, Yui Shiga, Takashi Kawaguchi, Shuji Tani","doi":"10.1002/jobm.202400266","DOIUrl":"10.1002/jobm.202400266","url":null,"abstract":"<div>\u0000 \u0000 The production of cellulolytic enzymes in Aspergillus aculeatus is regulated at transcriptional levels in response to inducers and various physiological signals. In this study, we identified that a component of the septation initiation network complex, SepL, a putative protein kinase, was involved in the expression of carbohydrate-active enzyme (CAZyme) encoding genes. The deletion of sepL (ΔsepL) in A. aculeatus resulted in a deficiency in both septation and conidiation and sensitivity to Congo red. These phenotypes of ΔsepL are conserved in Aspergillus. In addition to the conserved function of SepL in Aspergillus, we found that SepL in A. aculeatus was necessary for the inducible expression of the CAZyme genes in response to cellobiose, whereas the inducible expression of these genes in response to 1,4-β-mannobiose was significantly reduced but not abolished. Combining the results of the present functional analysis of SepL with previous evidence that the expression of the CAZyme genes, which is responsive to both cellobiose and 1,4-β-mannobiose, is regulated by a transcription factor ManR in A. aculeatus, indicates that SepL in A. aculeatus is involved in the selective expression of the cellobiose-responsive CAZyme genes under the control of ManR.</div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0