Journal of Basic Microbiology最新文献_第9页

Novel report on soil infection with Metarhizium rileyi against soil-dwelling life stages of insect pests 新报告：Metarhizium rileyi 对害虫在土壤中生活阶段的土壤感染。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-05-21 DOI: 10.1002/jobm.202400159

Perumal Vivekanandhan, Chinnaperumal Kamaraj, Sulaiman A. Alharbi, Mohammad J. Ansari

{"title":"Novel report on soil infection with Metarhizium rileyi against soil-dwelling life stages of insect pests","authors":"Perumal Vivekanandhan, Chinnaperumal Kamaraj, Sulaiman A. Alharbi, Mohammad J. Ansari","doi":"10.1002/jobm.202400159","DOIUrl":"10.1002/jobm.202400159","url":null,"abstract":"Entomopathogenic fungi are the most effective control remedy against a wide range of medical and agricultural important pests. The present study aimed to isolate, identify, and assess the virulence of Metarhizium rileyi against Spodoptera litura and Spodoptera frugiperda pupae under soil conditions. The biotechnological methods were used to identify the isolate as M. rileyi. The fungal conidial pathogenicity (2.0 × 107, 2.0 × 108, 2.0 × 109, 2.0 × 1010, and 2.0 × 1011 conidia/mL−1) was tested against prepupae of S. litura and S. frugiperda at 3, 6, 9, and 12 days after treatments. Additionally, the artificial soil-conidial assay was performed on a nontarget species earthworm Eudrilus eugeniae, using M. rileyi conidia. The present results showed that the M. rileyi caused significant mortality rates in S. litura pupae (61-90%), and S. litura pupae were more susceptible than S. frugiperda pupae (46%–73%) at 12 day posttreatment. The LC50 and LC90 of M. rileyi against S. litura, were 3.4 × 1014–9.9 × 1017 conidia/mL−1 and 6.6 × 105–4.6 × 1014 conidia/mL−1 in S. frugiperda, respectively. The conidia of M. rileyi did not exhibit any sublethal effect on the adult stage of E. eugeniae, and Artemia salina following a 12-day treatment period. Moreover, in the histopathological evaluation no discernible harm was observed in the gut tissues of E. eugeniae, including the lumen and epithelial cells, as well as the muscles, setae, nucleus, mitochondria, and coelom. The present findings provide clear evidence that M. rileyi fungal conidia can be used as the foundation for the development of effective bio-insecticides to combat the pupae of S. litura and S. frugiperda agricultural pests.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"64 8","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced polysaccharide production through quorum sensing system in Cordyceps militaris 冬虫夏草通过法定人数感应系统提高多糖产量

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-05-21 DOI: 10.1002/jobm.202400103

Huang Qiao, Jianshu Chen, Shengli Yang

{"title":"Enhanced polysaccharide production through quorum sensing system in Cordyceps militaris","authors":"Huang Qiao, Jianshu Chen, Shengli Yang","doi":"10.1002/jobm.202400103","DOIUrl":"10.1002/jobm.202400103","url":null,"abstract":"This study aimed to enhance extracellular polysaccharide (EPS) production in Cordyceps militaris by constructing a quorum sensing (QS) system to regulate the expression of biosynthetic enzyme genes, including phosphoglucomutase, hexokinase, phosphomannomutase, polysaccharide synthase, and UDP-glucose 4-epimerase genes. The study found higher EPS concentrations in seven recombinant strains compared to the wild-type C. militaris, indicating that the overexpression of key enzyme genes increased EPS production. Among them, the CM-pgm-2 strain exhibited the highest EPS production, reaching a concentration of 3.82 ± 0.26 g/L, which was 1.52 times higher than the amount produced by the wild C. militaris strain. Additionally, the regulatory effects of aromatic amino acids on the QS system of the CM-pgm-2 strain were investigated. Under the influence of 45 mg/L tryptophan, the EPS production in CM-pgm-2 reached 4.75 ± 0.20 g/L, representing a 1.90-fold increase compared to wild C. militaris strains. This study provided an effective method for the large-scale production of EPSs in C. militaris, and opened up new avenues for research into fungal QS mechanisms.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"64 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141070977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rhodotorula mucilaginosa A8, a potential helper strain in a vitamin C microbial fermentation process 黏液藻 A8，维生素 C 微生物发酵过程中的潜在辅助菌株。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-05-15 DOI: 10.1002/jobm.202400132

Qian Zhang, Lin Liao, Shuxia Lyu

{"title":"Rhodotorula mucilaginosa A8, a potential helper strain in a vitamin C microbial fermentation process","authors":"Qian Zhang, Lin Liao, Shuxia Lyu","doi":"10.1002/jobm.202400132","DOIUrl":"10.1002/jobm.202400132","url":null,"abstract":"In the vitamin C microbial fermentation system, oxidative stress limits the growth and 2-keto-l-gulonic acid (2-KLG, the precursor of vitamin C) production of Ketogulonicigenium vulgare. Most Bacillus strains, as helper strains, have been reported to release key biomolecules to reduce oxidative stress and promote the growth and 2-KLG production of K. vulgare. To understand the specific mechanism by which the helper strain and K. vulgare interact to reduce oxidative stress, a novel helper strain, Rhodotorula mucilaginosa A8, was used to construct a consortium in the co-culture fermentation system. Based on the activities of the antioxidant enzymes and quantitative polymerase chain reaction (qPCR) analysis, R. mucilaginosa A8 could reduce oxidative stress and increase 2-KLG production in K. vulgare by upregulating antioxidant enzyme activities and related gene-expression levels. In addition, the carotenoids of R. mucilaginosa promoted 2-KLG production in K. vulgare. Coculture of R. mucilaginosa with K. vulgare increased the yield of carotenoids. This study suggested that helper strains with the ability to reduce oxidative stress in K. vulgare would likely act as potential helper strains for facilitating 2-KLG biosynthesis. This work could provide a theoretical basis for the search for potential helper strains for vitamin C microbial fermentation and for the construction of synthetic microbial communities to produce valuable products.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"64 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140944234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antarctica's hidden mycoviral treasures in fungi isolated from mosses: A first genomic approach 从苔藓中分离出的真菌中隐藏的南极真菌病毒宝藏：第一种基因组学方法

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-05-12 DOI: 10.1002/jobm.202300671

Steffany V. A. Nobre, Guilherme A. K. de Andrade, Geferson F. Metz, Fabíola Lucini, Margéli P. de Albuquerque, Filipe de C. Victória

{"title":"Antarctica's hidden mycoviral treasures in fungi isolated from mosses: A first genomic approach","authors":"Steffany V. A. Nobre, Guilherme A. K. de Andrade, Geferson F. Metz, Fabíola Lucini, Margéli P. de Albuquerque, Filipe de C. Victória","doi":"10.1002/jobm.202300671","DOIUrl":"10.1002/jobm.202300671","url":null,"abstract":"This study investigates the presence of mycoviruses in Antarctic fungi and elucidates their evolutionary relationships. To achieve this, we aligned mycoviral gene sequences with genomes of previously sequenced Antarctic endophytic fungi, made available by our research group and accessible via Joint Genome Institute. Our findings reveal that the most prevalent genetic regions in all endophytic fungi are homologous to Partitiviruses, Baculoviridae, and Phycodnaviridae. These regions display evidence of positive selection pressure, suggesting genetic diversity and the accumulation of nonsynonymous mutations. This phenomenon implies a crucial role for these regions in the adaptation and survival of these fungi in the challenging Antarctic ecosystems. The presence of mycoviruses in Antarctic endophytic fungi may indicate shared survival strategies between the virus and its host, shedding light on their evolutionary dynamics. This study underscores the significance of exploring mycoviruses within endophytic fungi and their contributions to genetic diversity. Future research avenues could delve into the functional implications of these conserved mycoviral genetic regions in Antarctic endophytic fungi, providing a comprehensive understanding of this intriguing association and genomic retention of viral region in fungi.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"64 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140910953","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioprospecting of endophytic fungi from semidesert candelilla (Euphorbia antisyphilitica Zucc): Potential for extracellular enzyme production 半荒漠蜡烛草（Euphorbia antisyphilitica Zucc）内生真菌的生物勘探：生产细胞外酶的潜力。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-05-07 DOI: 10.1002/jobm.202400049

Itzel C. Núñez-García, Guillermo Cristian G. Martínez-Ávila, Silvia M. González-Herrera, Julio C. Tafolla-Arellano, O. Miriam Rutiaga-Quiñones

{"title":"Bioprospecting of endophytic fungi from semidesert candelilla (Euphorbia antisyphilitica Zucc): Potential for extracellular enzyme production","authors":"Itzel C. Núñez-García, Guillermo Cristian G. Martínez-Ávila, Silvia M. González-Herrera, Julio C. Tafolla-Arellano, O. Miriam Rutiaga-Quiñones","doi":"10.1002/jobm.202400049","DOIUrl":"10.1002/jobm.202400049","url":null,"abstract":"Endophytic microbial communities colonize plants growing under various abiotic stress conditions. Candelilla (Euphorbia antisyphilitica Zucc.) is a shrub that develops functionally in arid and semi-arid zones of Mexico; these conditions generate an association between the plant and the microorganisms, contributing to the production of enzymes as a defense mechanism for resistance to abiotic stress. The objective of this research was to isolate and identify endophyte fungi of candelilla and bioprospection of these endophytic fungi for enzyme production using candelilla by-products. Fungi were isolated and identified using ITS1/ITS4 sequencing. Their potency index (PI) was evaluated in producing endoglucanase, xylanase, amylase, and laccase. Fermentation was carried out at 30°C for 8 days at 200 rpm, with measurements every 2 days, using candelilla by-products as substrate. All fungi exhibited higher cellulase, amylase, and laccase activities on the 2nd, 6th, and 8th day of fermentation, respectively, of fermentation. The fungus Aspergillus niger ITD-IN4.1 showed the highest amylase activity (246.84 U/mg), the genus Neurospora showed the highest cellulase activity, reaching up to 13.45 FPU/mg, and the strain Neurospora sp. ITD-IN5.2 showed the highest laccase activity (3.46 U/mg). This work provides the first report on the endophytic diversity of E. antisyphilitica and its potential role in enzyme production.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"64 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140876553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Wastewater monitoring of SARS-CoV-2 gene for COVID-19 epidemiological surveillance in Tucumán, Argentina 阿根廷图库曼为 COVID-19 流行病监测而进行的 SARS-CoV-2 基因废水监测。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-05-07 DOI: 10.1002/jobm.202300773

María Cecilia D'Arpino, Pedro Eugenio Sineli, Gustavo Goroso, William Watanabe, María Lucila Saavedra, Elvira María Hebert, María Alejandra Martínez, Julieta Migliavacca, Silvina Gerstenfeld, Rossana Elena Chahla, Augusto Bellomio, Virginia Helena Albarracín

{"title":"Wastewater monitoring of SARS-CoV-2 gene for COVID-19 epidemiological surveillance in Tucumán, Argentina","authors":"María Cecilia D'Arpino, Pedro Eugenio Sineli, Gustavo Goroso, William Watanabe, María Lucila Saavedra, Elvira María Hebert, María Alejandra Martínez, Julieta Migliavacca, Silvina Gerstenfeld, Rossana Elena Chahla, Augusto Bellomio, Virginia Helena Albarracín","doi":"10.1002/jobm.202300773","DOIUrl":"10.1002/jobm.202300773","url":null,"abstract":"Wastewater-based epidemiology provides temporal and spatial information about the health status of a population. The objective of this study was to analyze and report the epidemiological dynamics of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) in the province of Tucumán, Argentina during the second and third waves of coronavirus disease 2019 (COVID-19) between April 2021 and March 2022. The study aimed to quantify SARS-CoV-2 RNA in wastewater, correlating it with clinically reported COVID-19 cases. Wastewater samples (n = 72) were collected from 16 sampling points located in three cities of Tucumán (San Miguel de Tucumán, Yerba Buena y Banda del Río Salí). Detection of viral nucleocapsid markers (N1 gene) was carried out using one-step reverse transcription-quantitative polymerase chain reaction (RT-qPCR). Viral loads were determined for each positive sample using a standard curve. A positive correlation (p < 0.05) was observed between viral load (copies/mL) and the clinically confirmed COVID-19 cases reported at specific sampling points in San Miguel de Tucumán (SP4, SP7, and SP8) in both months, May and June. Indeed, the high viral load concurred with the peaks of COVID-19 cases. This method allowed us to follow the behavior of SARS-CoV-2 infection during epidemic outbreaks. Thus, wastewater monitoring is a valuable epidemiological indicator that enables the anticipation of increases in COVID-19 cases and tracking the progress of the pandemic. SARS-CoV-2 genome-based surveillance should be implemented as a routine practice to prepare for any future surge in infections.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"64 8","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140856475","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Linalool-zinc oxide nanocomposite controls Toxoplasma gondii infection through inhibiting inflammation, oxidative stress, and pathogenicity 芳樟醇-氧化锌纳米复合材料通过抑制炎症、氧化应激和致病性控制弓形虫感染

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-05-01 DOI: 10.1002/jobm.202400039

Hanadi B. A. Baghdadi, Aishah E. Albalawi, Abdullah F. Shater, Hamdan Almohammed, Abdullah D. Alanazi

{"title":"Linalool-zinc oxide nanocomposite controls Toxoplasma gondii infection through inhibiting inflammation, oxidative stress, and pathogenicity","authors":"Hanadi B. A. Baghdadi, Aishah E. Albalawi, Abdullah F. Shater, Hamdan Almohammed, Abdullah D. Alanazi","doi":"10.1002/jobm.202400039","DOIUrl":"10.1002/jobm.202400039","url":null,"abstract":"The present in vitro and in vivo study aimed to fabricate and characterize linalool-zinc oxide nanoparticles (Lin-ZNP) and evaluate their effectiveness against Toxoplasma gondii infection in terms of inflammation, oxidative stress, and pathogenicity. Lin-ZNP was synthesized using an ethanolic solution of polyvinyl alcohol. The anti-Toxoplasma and cytotoxicity activities of Lin-ZNP were investigated, along with its effects on nitric oxide (NO) production, caspase-3 activity, and pro-inflammatory genes. After treating T. gondii-infected mice with Lin-ZNP for 14 days, the number and size of tissue cysts, antioxidant potential, pro-inflammatory cytokines, and T. gondii pathogenicity-related genes were evaluated by real-time polymerase chain reaction and Western blot analysis. The Lin-ZNP composite showed a reduced tendency with an average size of 105 nm. Lin-ZNP significantly reduced the viability of tachyzoites. The obtained selectivity index higher than 10, indicating high specificity for parasites with low cytotoxicity to normal cells. The Lin-ZNP significantly (p < 0.05) increased the production of NO, caspase-3 activity, and the expression levels of pro-inflammatory genes. Lin-ZNP significantly (p < 0.001) decreased the size and number of tissue cysts and caused a significant reduction in the level of malondialdehyde and a considerable increase (p < 0.001) in antioxidant enzymes and their expression genes. Lin-ZNP significantly downregulated both mRNA and protein expression of the inflammation-related markers associated with the TLRs/NF-κB pathway. The expression levels of the T. gondii pathogenicity-related genes were significantly downregulated (p < 0.05). The recent survey indicated that Lin-ZNP manages T. gondii infection by its antioxidant activity and inhibiting the TLRs/NF-κB pathway without toxicity in mice.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"64 8","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140834624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cover: Journal of Basic Microbiology. 5/2024 封面：基础微生物学杂志》。5/2024

IF 3.1 4区生物学

Journal of Basic Microbiology Pub Date : 2024-05-01 DOI: 10.1002/jobm.202470041

引用次数: 0

Issue Information: Journal of Basic Microbiology. 5/2024 期刊信息：基础微生物学杂志》。5/2024

IF 3.1 4区生物学

Journal of Basic Microbiology Pub Date : 2024-05-01 DOI: 10.1002/jobm.202470042

引用次数: 0

Ganoderma lucidum: Insights on host range, diagnosis, and management strategies 灵芝：关于宿主范围、诊断和管理策略的见解

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2024-04-30 DOI: 10.1002/jobm.202300769

Ashwani Kumari, Ashwani Tapwal, Neha Thakur

{"title":"Ganoderma lucidum: Insights on host range, diagnosis, and management strategies","authors":"Ashwani Kumari, Ashwani Tapwal, Neha Thakur","doi":"10.1002/jobm.202300769","DOIUrl":"10.1002/jobm.202300769","url":null,"abstract":"Forest ecosystems play an important role in upholding life on our planet. However, the onslaught of fungal pathogens like Ganoderma lucidum, poses a threat by decimating numerous tree species. G. lucidum identified as a root pathogen, causing root rot in numerous tree species of horticulture and forestry importance. The fungus initiates infection through basidiospores, which germinate and penetrate within roots and start to degrade lignocellulosic components of plant cells. Early-stage detection of G. lucidum, is challenging, while in advance stages, the wood undergoes softening and a loss of tensile strength, rendering the disease incurable. Hence, effective management of G. lucidum necessitates a pivotal role of disease diagnostic techniques, which are currently underutilized or inadequately accessible. Subsequent implementation of suitable control measures becomes imperative to thwart disease occurrence and mitigate its impact in early stages, thus preserving the vitality of forest ecosystems. This study provides comprehensive overview of G. lucidum, covering taxonomy, pathogenicity, disease cycle, diagnosis and effective control measures, which will be helpful in formulating effective diagnostic techniques for early management of root rot disease.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"64 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.202300769","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140835006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0