Journal of Basic Microbiology最新文献_第9页

Assessment of Physiological Traits in Cyanobacteria Isolated From Rice Agroecosystems for Biofertilizer Applications 水稻农业生态系统分离蓝藻生理性状评价及其在生物肥料上的应用。

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-01-21 DOI: 10.1002/jobm.70138

Anjali Singh, Kamakshi Saxena, Devendra Kumar

{"title":"Assessment of Physiological Traits in Cyanobacteria Isolated From Rice Agroecosystems for Biofertilizer Applications","authors":"Anjali Singh, Kamakshi Saxena, Devendra Kumar","doi":"10.1002/jobm.70138","DOIUrl":"10.1002/jobm.70138","url":null,"abstract":"<div>\u0000 \u0000 Cyanobacteria are photosynthetic prokaryotes recognized for their varying pigment composition and capacity to adapt to different ecological situations. Even though they are significant, there aren't many studies comparing native cyanobacterial strains in terms of their ability to be used as biofertilizers. This study comparatively evaluated the physiological characteristics of seven cyanobacterial strains (Nostoc sp., Anabaena sp., Westiellopsis sp., Oscillatoria sp., Tolypothrix sp., Calothrix sp., and Phormidium sp.) isolated from paddy fields in different regions of Uttar Pradesh (Meerut, Muzaffarnagar, and Haridwar regions). The examined parameters included photosynthetic pigments- chlorophyll, carotenoids, phycobiliproteins-Phycocyanin (PC), Phycoerythrin (PE), and Allophycocyanin (APC), Total Soluble Protein (TSP), and carbohydrates. Cultures were cultivated in BG-11 medium, and samples were analyzed at 7th, 14th, 21st, and 28th days of incubation for examination via spectrophotometer. The findings indicated substantial interstrain differences. Anabaena sp. demonstrated the highest levels of chlorophyll, carotenoids, proteins, and carbohydrates on Day 14, while Tolypothrix sp. had the greatest concentrations of APC and PE. The strain-specific variations underscore the metabolic diversity of paddy field cyanobacteria. This thorough comparison of physiological data sheds light on the adaptation mechanisms of cyanobacteria and their potential uses as natural biofertilizers and in biotechnological applications.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146010542","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bacillus Consortium Promotes the Growth of Pea Plants and Protects Against Fusarium Wilt Disease 芽孢杆菌联合体促进豌豆植株生长和防治枯萎病。

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-01-21 DOI: 10.1002/jobm.70142

Zainab Shabbir, Waheed Akram, Rameen Rafique, Syeda Kanz-Ul-Eman, Tehmina Anjum, Zill-e-Huma Aftab, Adnan Akhter, Basharat Ali

{"title":"Bacillus Consortium Promotes the Growth of Pea Plants and Protects Against Fusarium Wilt Disease","authors":"Zainab Shabbir, Waheed Akram, Rameen Rafique, Syeda Kanz-Ul-Eman, Tehmina Anjum, Zill-e-Huma Aftab, Adnan Akhter, Basharat Ali","doi":"10.1002/jobm.70142","DOIUrl":"10.1002/jobm.70142","url":null,"abstract":"<div>\u0000 \u0000 Fusarium wilt disease is a significant challenge to the tomato crop, causing economic losses worldwide. The unwise use of chemical fungicides has raised concerns about food safety. Hence, biocontrol is a sustainable strategy for controlling Fusarium wilt disease. This study aimed to promote growth and manage Fusarium wilt disease in pea plants using two Bacillus strains (Bacillus aryabhattai strain Z-48 and Bacillus cereus strain Z-53) either alone or in a synthetic consortium. The application of the consortium of both Bacillus strains provided maximum protection against Fusarium wilt disease, showing the biocontrol effect of 54.3%. Under Fusarium disease stress, the consortium application increased shoot length, root length and dry biomass up to 88.6%, 31.1%, and 86.7% respectively, compared with the pathogen alone treatment. Likewise, the application of the Bacillus consortium significantly increased the time-course accumulation of defence-related enzymes, and photosynthetic pigments in Pea plants. Non-targeted metabolite profiling indicated extensive remodulations in the production of a wide array of metabolites upon application of the consortium (Z-48 + Z-53). The multivariate analysis showed strong relationships between treatments and different metabolites, including phenylalanine, ursolic acid, and glycerol-3-phosphocholine. The study demonstrates that this Bacillus consortium can be effectively used to develop a biocontrol-based formulation for farming applications.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146018760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Identification and Pathogenicity of Novel Tomato Root Rot Fungi, With Insights Into Cultivar Resistance in Saudi Arabia 沙特阿拉伯番茄新型根腐病真菌的分子鉴定、致病性及品种抗性研究。

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-01-18 DOI: 10.1002/jobm.70139

Khalid E. Hamed, Ali M. Haddadi, Ayman F. Omar, Mona Saleh Al Tami, Ritu Rani, Rajesh N. Udavant, Suhail Ashraf

{"title":"Molecular Identification and Pathogenicity of Novel Tomato Root Rot Fungi, With Insights Into Cultivar Resistance in Saudi Arabia","authors":"Khalid E. Hamed, Ali M. Haddadi, Ayman F. Omar, Mona Saleh Al Tami, Ritu Rani, Rajesh N. Udavant, Suhail Ashraf","doi":"10.1002/jobm.70139","DOIUrl":"10.1002/jobm.70139","url":null,"abstract":"<div>\u0000 \u0000 Tomato (Solanum lycopersicum L.) is a major horticultural crop worldwide but remains highly vulnerable to soil-borne fungal pathogens that threaten greenhouse production. Increasing incidences of root rot and wilt have been observed in tomato nurseries and greenhouses in the Qassim and Riyadh regions of Saudi Arabia. This study aimed to identify the causal fungal agents and assess cultivar resistance under controlled conditions. Symptomatic root samples were collected, and pathogens were isolated, morphologically characterized, and confirmed through internal transcribed spacer (ITS) sequencing. Pathogenicity tests verified Fusarium oxysporum, Fusarium foetens, Ectophoma multirostrata, and Pygmaeomyces thomasii as causal agents of tomato root rot and wilt. Notably, E. multirostrata and P. thomasii were recorded for the first time as tomato root pathogens in Saudi Arabia. Four commercial tomato cultivars (Ace 55 VF, Marmande, Rio Grande, and Red Cherry) were evaluated for resistance. Disease incidence, severity, and growth parameters revealed significant variability among cultivars, with ‘Red Cherry’ and ‘Rio Grande’ showing enhanced resistance compared to more susceptible varieties. These results emphasize the novelty of detecting two previously unreported pathogens in Saudi Arabia and demonstrate the practical relevance of identifying resistant cultivars for field application. Overall, the study provides valuable insights supporting sustainable tomato production and offers a foundation for future resistance-breeding strategies.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxy-Imino-Cephalosporin Induces Expression of Secondary Resistance Genes repB and mtgA in Escherichia coli Harboring blaCTX-M ESBL 氧基亚氨基头孢菌素诱导携带blaCTX-M ESBL的大肠杆菌中reb和mtgA基因的表达

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2025-12-29 DOI: 10.1002/jobm.70135

Nikita Karmakar, Jayalaxmi Wangkheimayum, Debadatta Dhar Chanda, Manabendra Dutta Choudhury, Amitabha Bhattacharjee

{"title":"Oxy-Imino-Cephalosporin Induces Expression of Secondary Resistance Genes repB and mtgA in Escherichia coli Harboring blaCTX-M ESBL","authors":"Nikita Karmakar, Jayalaxmi Wangkheimayum, Debadatta Dhar Chanda, Manabendra Dutta Choudhury, Amitabha Bhattacharjee","doi":"10.1002/jobm.70135","DOIUrl":"10.1002/jobm.70135","url":null,"abstract":"<div>\u0000 \u0000 Escherichia coli is considered a major extended-spectrum β-lactamases producer, of which CTX-M is the most common member. However, the different variants of this primary resistance-determinant make it challenging to detect by conventional PCR-based techniques. Therefore, in this study, we investigated the transcriptional response of 2 secondary resistance genes, repB and mtgA, under sub-inhibitory concentrations of oxy-imino cephalosporins in resistant isolates of E. coli harboring blaCTX-M. The STRING database and Cytoscape software were used to identify the secondary resistance genes by gene network analysis. The cephalosporin-resistant isolate of E. coli co-harboring repB and mtgA, along with blaCTX-M, was employed for the current study. The blaCTX-M-carrying plasmid was successfully eliminated from the native-type isolate using 6% and 8% SDS treatment for 7 days. The plasmid was also transformed into a recipient strain, E. coli DH5α, by the heat-shock method. All three models were grown under sub-inhibitory stress of cephalosporins, and the transcriptional response of repB and mtgA was determined by quantitative real-time PCR. The study revealed that the transcriptional responses of both repB and mtgA were antibiotic-specific, which potentially can serve as a secondary resistance reporter for cephalosporin resistance in E. coli.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-12-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145849359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genomics and Synthetic Microbiology in Environmental Interactions 环境相互作用中的基因组学和合成微生物学

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2025-12-15 DOI: 10.1002/jobm.70136

Erika Kothe

引用次数: 0

Advancement in Mushroom Transformation: From Conventional Techniques to Modern Genetic Engineering 蘑菇转化的进展：从传统技术到现代基因工程。

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2025-12-15 DOI: 10.1002/jobm.70132

Kanwarpreet Singh, Shivani Sharma, Anu Kalia, Pooja Manchanda

{"title":"Advancement in Mushroom Transformation: From Conventional Techniques to Modern Genetic Engineering","authors":"Kanwarpreet Singh, Shivani Sharma, Anu Kalia, Pooja Manchanda","doi":"10.1002/jobm.70132","DOIUrl":"10.1002/jobm.70132","url":null,"abstract":"<div>\u0000 \u0000 Mushrooms have long been valued for their nutritional, pharmaceutical, and culinary benefits. Recent studies showcased mushrooms as bio-factories for protein production, and as a source of value-added products by employing genetic manipulation and molecular transformation techniques. Advancements in molecular tools and transformation methods have enhanced the efficiency of genetic improvements in mushrooms by both conventional and modern genetic engineering techniques, paving the way for their use in various industrial applications. Genetic transformation in mushrooms involves transferring genes within and across species to understand gene functions and improve mushroom qualities. The techniques involved in transformation includes Agrobacterium-mediated transformation, hybridization, mutation breeding, particle bombardment, protoplast fusion, and CRISPR/Cas9. This review outlines the life cycle of mushrooms, major difficulties in mushroom transformation, various transformation techniques, their history, efficiency, and success rate. It also highlights the potential of genetic engineering to revolutionize mushroom cultivation and their applications.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 1","pages":""},"PeriodicalIF":2.7,"publicationDate":"2025-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145762296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Journal of Basic Microbiology. 1/2026 期刊信息：Journal of Basic Microbiology. 1/2026

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2025-12-12 DOI: 10.1002/jobm.70137