Journal of Basic Microbiology最新文献_第8页

Mobilome-Mediated Speciation: Genomic Insights Into Horizontal Gene Transfer in Methanosarcina 移动组介导的物种形成：甲烷菌水平基因转移的基因组见解。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-06 DOI: 10.1002/jobm.70013

Sivashanmuga Vadivel Saranya, R. Prathiviraj, Paulchamy Chellapandi

{"title":"Mobilome-Mediated Speciation: Genomic Insights Into Horizontal Gene Transfer in Methanosarcina","authors":"Sivashanmuga Vadivel Saranya, R. Prathiviraj, Paulchamy Chellapandi","doi":"10.1002/jobm.70013","DOIUrl":"10.1002/jobm.70013","url":null,"abstract":"<div>\u0000 \u0000 Speciation in prokaryotes is often driven by complex genetic exchanges such as horizontal gene transfer (HGT), which facilitates genomic divergence and adaptation. In this study, we inferred the evolutionary transitions of the mobilome (plasmids, transposons, and phages) between Methanosarcina and bacteria in driving speciation within the Methanosarcina genus. By conducting evolutionary and phylogenetic analyses of Methanosarcina acetivorans, M. barkeri, M. mazei, and M. siciliae, we identified key mobilome elements acquired through HGT from distantly related bacterial species. These mobile genetic elements have shaped genomic plasticity, enabling Methanosarcina to adapt to diverse environmental niches and potentially facilitating lineage divergence. The acquisition of mobilome-associated genes involved in antibiotic resistance, DNA repair, and stress responses suggests their significant role in the ecological speciation of Methanosarcina. Overall, we hypothesized that their mobile genetic element might have been acquired from distantly related bacteria by HGT and subsequently established as new functional homologs in the present lineage. This study provides insight into how mobilome-mediated gene flow contributes to genomic divergence and speciation within microbial populations, highlighting the broader significance of mobilome in microbial evolution and speciation processes.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fermentation Optimization of Loonamycin Production by Marine Nocardiopsis flavescens 海洋 Nocardiopsis flavescens 生产卢安霉素的发酵优化。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-06 DOI: 10.1002/jobm.70012

Tianyu Zhou, Yuan Cao, Zhicheng Zhang, Jing Shi, Huiming Ge, Faliang An, Jiang Ye, Ruida Wang, Haizhen Wu

{"title":"Fermentation Optimization of Loonamycin Production by Marine Nocardiopsis flavescens","authors":"Tianyu Zhou, Yuan Cao, Zhicheng Zhang, Jing Shi, Huiming Ge, Faliang An, Jiang Ye, Ruida Wang, Haizhen Wu","doi":"10.1002/jobm.70012","DOIUrl":"10.1002/jobm.70012","url":null,"abstract":"<div>\u0000 \u0000 The antitumor drug candidate, loonamycin, was isolated from Nocardiopsis flavescens NA01583 and exerts its antitumor activity by inhibiting topoisomerase I activity. However, the current production levels of loonamycin are insufficient for further research. To address this limitation, a liquid fermentation method was developed, optimizing both fermentation conditions and medium components. This optimization increased loonamycin production from 1 mg/mL on agar plate to 602.32 mg/mL in shake flask and ultimately 532.51 mg/mL in a 5-L bioreactor. Exogenous addition experiments revealed that sea salt and metal ions significantly inhibit loonamycin synthesis. Multi-temporal transcriptomic analyses indicated that the reduced expression levels of genes involved in the shikimate pathway and L-tryptophan biosynthesis pathway were the limiting factors for increased loonamycin yield. Overall, an effective fermentation method for rare marine actinomycetes was established, significantly enhancing loonamycin production and providing a foundation for the development of this novel antitumor prodrug.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimized Production and Characterization of Auxin by Bacillus amyloliquefaciens (TSP11) for Plant Growth Promotion 解淀粉芽孢杆菌（TSP11）促植物生长生长素的优化及特性研究。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-06 DOI: 10.1002/jobm.70010

Thangarasu Suganya Devi, Karuppiah Vijay, Raja Mythili, Mohammad Ahmad Wadaan, Vasubalan Manivinoth, Woong Kim, Thangavel Kavitha

{"title":"Optimized Production and Characterization of Auxin by Bacillus amyloliquefaciens (TSP11) for Plant Growth Promotion","authors":"Thangarasu Suganya Devi, Karuppiah Vijay, Raja Mythili, Mohammad Ahmad Wadaan, Vasubalan Manivinoth, Woong Kim, Thangavel Kavitha","doi":"10.1002/jobm.70010","DOIUrl":"10.1002/jobm.70010","url":null,"abstract":"<div>\u0000 \u0000 This study was aimed at isolating and recognizing the plant growth promoting rhizobacteria that secretes indole-3-acetic acid/auxin hormones. Among the 82 pure cultured bacterial morphotypes, the strain TSP11 showed high auxin production utilizing rice husk as a cheap substitute in the production medium. The minimal medium supplemented with 0.1 g/mL L-tryptophan and 1.29% rice husk produced the highest amount of auxin. Synthesized auxin was purified and quantified by chromatographic techniques. 120.08 µg/mL of synthetic medium and 135 µg/mL of agro-waste medium were the observed yields in HPLC quantification. Further GC-MS/MS and LC-MS/MS spectra exposed an existence of derived compounds such as 1H-indole, 7-methyl, indole, 3-methyl-, 1H-indole-3-carboxaldehyde (1H-indol-3-YL)-acetic acid. It was characterized by X-ray diffraction to reveal its crystallinity. Thermo gravimetric analysis showed the thermal stability of auxin over the range between 200°C and 300°C. Structural predictions of extracted auxin through HR-NMR revealed chemical shifts for Indole and acetate derivatives. The potent producer was identified as Bacillus amyloliquefaciens strain TSP11based on 16SrDNA gene sequencing. Increased seed germination percentage was observed when compared to untreated control. The research findings explore the possibility of exploiting B. amyloliquefaciens strain TSP11for bioformulations. This study represents an initial investigation into the plant growth-promoting potential of B. amyloliquefaciens strain TSP11, particularly regarding its auxin production using rice husk as a medium. We will explore the exact mechanism of auxin synthesis and its impact on various plant species in further studies.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 5","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial Art Fixation on Agar Plate: MicArt Fixation for Microbiology Teaching 琼脂平板微生物艺术固定术：微生物学教学用MicArt固定术。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-06 DOI: 10.1002/jobm.70018

Hatice Nur Halipci Topsakal, Murat Can Asarkaya, Ecem Akcan, Melih Çifçi, Rojda Karaman, Sudenaz Doğan, Elif Nur Çelik, Betül Bakir, Yasemin Günter, Okan Aydoğan

{"title":"Microbial Art Fixation on Agar Plate: MicArt Fixation for Microbiology Teaching","authors":"Hatice Nur Halipci Topsakal, Murat Can Asarkaya, Ecem Akcan, Melih Çifçi, Rojda Karaman, Sudenaz Doğan, Elif Nur Çelik, Betül Bakir, Yasemin Günter, Okan Aydoğan","doi":"10.1002/jobm.70018","DOIUrl":"10.1002/jobm.70018","url":null,"abstract":"Art based science materials engage students for learning all of the unseen microscobic creatures in easy way microbiology field. In this study we aimed to visualize bacterial and fungal species in artistic way. Inoculation of the colourful strains on agar plate as painting the agar with bacteria, provides beautiful round scenes and clear reminiscence related with the species learning for microbe world. For this purpose, we determined the different concentrations and exposure times of formaldehyde, glutaraldehyde, xylene, toluene, ethyl alcohol, epoxy, glycerin and phenol. We aimed to develop a protocol after determining the optimum storage times. As a method, we exposed all chemicals except epoxy to Petri dishes by spraying method for 1, 5, 10 min. As a result, we determined the fixative that provides the best protection is toluene. After 1 min of toluene exposure, we determined that the Petri dish with growth can be preserved for 6 months even under room conditions without disturbing the color and shape of the whole colony morphology. Here, we obtained best fixation process by using toluen, typically 1 min, depending on the species, was most promising for the preservation of art.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 6","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.70018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143567229","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Journal of Basic Microbiology. 3/2025 期刊信息：Journal of Basic Microbiology. 3/2025

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-05 DOI: 10.1002/jobm.202570006

引用次数: 0

Cover: Journal of Basic Microbiology. 3/2025 封面：Journal of Basic Microbiology. 3/2025

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-05 DOI: 10.1002/jobm.202570005

引用次数: 0

Harnessing Microbial Consortia for Sustainable Management of Corm Rot of Saffron. 利用微生物群落对藏红花茎腐病进行可持续管理。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-02-25 DOI: 10.1002/jobm.70009

Ayushi Sharma, Vishal Gupta, Gayatri Jamwal, Sushil K Gupta, Vinod Pandit, Zakir Amin, Fayaz A Mohiddin, Khalid E Hamed, Suhail Ashraf

{"title":"Harnessing Microbial Consortia for Sustainable Management of Corm Rot of Saffron.","authors":"Ayushi Sharma, Vishal Gupta, Gayatri Jamwal, Sushil K Gupta, Vinod Pandit, Zakir Amin, Fayaz A Mohiddin, Khalid E Hamed, Suhail Ashraf","doi":"10.1002/jobm.70009","DOIUrl":"https://doi.org/10.1002/jobm.70009","url":null,"abstract":"Saffron (Crocus Sativus L.) is a highly prized spice crop renowned for its significant culinary and therapeutic applications, largely attributed to its bioactive constituents such as crocin, picrocrocin, and safranal. Despite its production is severely threatened by corm rot, particularly caused by the pathogenic fungus Fusarium oxysporum, which leads to substantial yield losses in major saffron-producing regions. This review highlights the promising role of microbial consortia as an eco-friendly and sustainable strategy for effectively managing corm rot disease. The presence of diverse microbial communities can improve defense mechanisms of a plant against pathogens and support its overall growth and productivity. The mechanisms by which these microbial interactions occur, including nutrient competition, biofilm formation, and the activation of systemic resistance, contribute to their combined efficacy in inhibiting fungal pathogens. The necessity for innovative microbial strategies that harness the combined benefits of diverse microorganisms to mitigate corm rot and improve saffron cultivation sustainably. As sustainable agricultural practices increasingly gain prominence, the development and application of tailored microbial consortia gave a promising alternative to chemical fungicides, ensuring both economic viability and ecological balance in saffron production.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70009"},"PeriodicalIF":3.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harnessing Fungal Bioagents Rich in Volatile Metabolites for Sustainable Crop Protection: A Critical Review 利用富含挥发性代谢物的真菌生物制剂进行可持续作物保护：综述

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-02-25 DOI: 10.1002/jobm.70003

Dheeraj Kumar, Somnath Roy, Azariah Babu, Abhay K. Pandey

{"title":"Harnessing Fungal Bioagents Rich in Volatile Metabolites for Sustainable Crop Protection: A Critical Review","authors":"Dheeraj Kumar, Somnath Roy, Azariah Babu, Abhay K. Pandey","doi":"10.1002/jobm.70003","DOIUrl":"10.1002/jobm.70003","url":null,"abstract":"<div>\u0000 \u0000 Pests and diseases have a significant impact on crop health and yields, posing a serious threat to global agriculture. Effective management strategies, such as integrated pest management (IPM), including crop rotation, use of synthetic pesticides, biological control, and resistant/tolerant crop varieties, are essential to mitigate these risks and ensure sustainable agricultural practices. Fungal bioagents play an important role in managing phytopathogens and insect pests by acting as biological agents. They promote healthy plant growth by enhancing the uptake of nutrients and combating systemic resistance in plants. Furthermore, fungal bioagents are environmentally friendly, reducing application of fungicides and insecticides and minimizing their negative impact on the crops and environment. Their use in IPM promotes sustainable agriculture and ensures high-quality crops while maintaining soil health and microbial biodiversity. These fungal bioagents are rich sources of volatile organic compounds (VOCs), which play an important role in biological communication during interaction with insect pests and phytopathogens. In pest management, VOC production by beneficial fungi is accountable for their efficacy against pests and pathogens. Thus, this review discusses the important fungal bioagents producing VOCs, extraction methods of VOC, and the use of VOC-producing fungi in pest and disease management, knowledge gaps, and future research areas.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomic Profiling and Genome-Wide Analysis of Bacillus subtilis NBAIR-BSWG1 Reveals Cyclic Lipopeptides as Key Antagonists 枯草芽孢杆菌nair - bswg1代谢组学分析和全基因组分析揭示环状脂肽是关键拮抗剂。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-02-23 DOI: 10.1002/jobm.70007

Ankitha Karegowdru Surendra, Radha Tumkur Kumaraswamy, Ruqiya Seegenahalli, Aditya Kukreti, Lavanya Siddanahalli Manjunatha, Kandan Aravindaram, Sivakumar Gopalsamy, Ramya Ramesan Syamala, Manjunatha Channappa

{"title":"Metabolomic Profiling and Genome-Wide Analysis of Bacillus subtilis NBAIR-BSWG1 Reveals Cyclic Lipopeptides as Key Antagonists","authors":"Ankitha Karegowdru Surendra, Radha Tumkur Kumaraswamy, Ruqiya Seegenahalli, Aditya Kukreti, Lavanya Siddanahalli Manjunatha, Kandan Aravindaram, Sivakumar Gopalsamy, Ramya Ramesan Syamala, Manjunatha Channappa","doi":"10.1002/jobm.70007","DOIUrl":"10.1002/jobm.70007","url":null,"abstract":"<div>\u0000 \u0000 Understanding bacterial genetics and metabolism is vital for developing biopesticides. This study investigates Bacillus subtilis NBAIR-BSWG1, a strain well known for its antagonistic potential. Crude lipopeptides extracted from the strain were evaluated for in vitro activity, showing complete inhibition of Rhizoctonia solani at a concentration of 50 μL/mL potato dextrose agar. To delve deeper into its antagonistic mechanisms, we conducted whole-genome sequencing of NBAIR-BSWG1 using Illumina NextSeq 500. Subsequent analysis with the BlastX diamond tool revealed 19 key biosurfactant genes, including surfactin (srfAA, srfAC, srfAD, srfP), fengycin (ppsE, ppsD, ppsC, ppsB), and putisolvin (dnaK), which were further confirmed by PCR using specific primers. Meanwhile, antiSMASH analysis revealed gene clusters with 100% similarity to those responsible for the synthesis of fengycin, bacilaene, bacillibactin, subtilosin A, and bacilysin, as well as clusters with 82% similarity to surfactin synthesis genes. Additionally, liquid chromatography-mass spectrometry was performed to analyze the cell-free extract produced by NBAIR-BSWG1, revealing the presence of various cyclic lipopeptides, including multiple peaks corresponding to surfactin, iturin, and several novel lipopeptide compounds. This study highlights B. subtilis NBAIR-BSWG1 cyclic lipopeptides as a key to broad-spectrum bio-control and establishes the strain as highly potent.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Interactive Dynamics of Pseudomonas aeruginosa in Global Ecology 全球生态中铜绿假单胞菌的相互作用动态。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-02-19 DOI: 10.1002/jobm.70004

Telma de Sousa, Catarina Silva, Gilberto Igrejas, Michel Hébraud, Patrícia Poeta

引用次数: 0