Journal of Basic Microbiology最新文献

{"title":"Harnessing Trichoderma asperellum: Tri-Trophic Interactions for Enhanced Black Gram Growth and Root Rot Resilience","authors":"Praveen Vijayakumar,&nbsp;Sudha Appusami,&nbsp;Selva Amala Anbazhagan,&nbsp;Ramjegathesh Rajendran,&nbsp;Kavitha Shanmugam,&nbsp;Kahkashan Perveen,&nbsp;Najat A. Bukhari,&nbsp;Riyaz Sayyed","doi":"10.1002/jobm.202400569","DOIUrl":"10.1002/jobm.202400569","url":null,"abstract":"<div>\u0000 \u0000 <p>Root rot caused by <i>Macrophomina phaseolina</i>, a common soil-borne disease in black gram, is managed with chemical fungicides, leading to toxicity and degradation of beneficial soil microbes. Existing bioagents, like talc formulation, cause leaching, clogging, and reduced productivity. The development of liquid bio-formulation via drip irrigation is crucial to mitigate biotic stress and maximize yield. This study aims to investigate the efficacy and survivability of liquid formulation of <i>Trichoderma asperellum</i> against root rot and its growth promotion. The results showed that Tv1 effectively inhibited <i>M. phaseolina</i> (66.67%), under in vitro condition. The vigor index of 4025.00 and the spore load of 1 × 10⁸ cfu/mL were recorded from plant growth promotion and spermosphere study @ 5 mL/kg of seeds with formulation. The study found that combined application of seed treatment @ 5 mL/kg of seed and soil application @ 10 mL/L of water significantly reduced disease incidence (9.1%) against control (74.3%), with increased biomass index. There are 32 mVOCs profiled during the tritrophic interaction in roots of black gram and they were up or downregulated, viz., mollugin, pentadecanoic acid, cyclopropaneoctanoic acid, 2-octyl-, methyl ester, rhodopin, dodecanoic acid, 1,2,3-propanetriyl ester by involved in defense mechanism and biosynthetic pathways like jasmonic acid, glyconeogenic and act as acyl-CoA: acyltransferase 2 inhibitor. The results of this study confirmed that liquid formulation performs better in growth promotion, survivability on seed surface, and managing root rot of black gram compared talc-based formulation.</p></div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 3","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Information: Journal of Basic Microbiology. 12/2024","authors":"","doi":"10.1002/jobm.202470112","DOIUrl":"https://doi.org/10.1002/jobm.202470112","url":null,"abstract":"","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"64 12","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.202470112","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Component of the Septation Initiation Network Complex, SepL, Participates in the Cellobiose-Responsive Expression of Cellulolytic Enzyme Genes in Aspergillus aculeatus.","authors":"Kazumi Sawada, Saki Kikuya, Yui Shiga, Takashi Kawaguchi, Shuji Tani","doi":"10.1002/jobm.202400266","DOIUrl":"https://doi.org/10.1002/jobm.202400266","url":null,"abstract":"<p><p>The production of cellulolytic enzymes in Aspergillus aculeatus is regulated at transcriptional levels in response to inducers and various physiological signals. In this study, we identified that a component of the septation initiation network complex, SepL, a putative protein kinase, was involved in the expression of carbohydrate-active enzyme (CAZyme) encoding genes. The deletion of sepL (ΔsepL) in A. aculeatus resulted in a deficiency in both septation and conidiation and sensitivity to Congo red. These phenotypes of ΔsepL are conserved in Aspergillus. In addition to the conserved function of SepL in Aspergillus, we found that SepL in A. aculeatus was necessary for the inducible expression of the CAZyme genes in response to cellobiose, whereas the inducible expression of these genes in response to 1,4-β-mannobiose was significantly reduced but not abolished. Combining the results of the present functional analysis of SepL with previous evidence that the expression of the CAZyme genes, which is responsive to both cellobiose and 1,4-β-mannobiose, is regulated by a transcription factor ManR in A. aculeatus, indicates that SepL in A. aculeatus is involved in the selective expression of the cellobiose-responsive CAZyme genes under the control of ManR.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e2400266"},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142769257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cover: Journal of Basic Microbiology. 12/2024","authors":"","doi":"10.1002/jobm.202470111","DOIUrl":"https://doi.org/10.1002/jobm.202470111","url":null,"abstract":"<p><b>Cover illustration:</b></p><p>Growth disc of an anamorphous fungus <i>Trichospsoron</i> sp. isolated from a rotten zucchini marketed. This phytopathogenic-pectinolytic fungus was tested against several strains of plant growth-promoting rhizobacteria to test its sensitivity to assess the capacity of five highly effective PGPR-<i>Bacillus</i> strains, which were obtained from the rhizosphere of date palms.</p><p>(Photo: Merdia Bestami, Department of Natural and Life Sciences, Faculty of Sciences and Technology, University of Amine Elokkal ElHadj Moussa Eg. Akhamoukh, Tamanrasset, Algeria)\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"64 12","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.202470111","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142762097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Mechanisms of Cell Death in Leishmania donovani Induced by Selected Steroidal Alkaloids","authors":"Naveena Menpadi,&nbsp;Pranjal Chandra,&nbsp;Vikash Kumar Dubey","doi":"10.1002/jobm.202400655","DOIUrl":"10.1002/jobm.202400655","url":null,"abstract":"<div>\u0000 \u0000 <p>We have earlier reported novel anti-leishmanial molecules, veratramine and hupehenine, targeting dephospho-coenzyme A kinase of the parasite. In our current investigation, we assessed the efficacy of these two steroidal alkaloids, veratramine and hupehenine, in combating the parasite. Contrary to expectations, our study did not detect the typical signs of apoptosis such as mitochondrial membrane potential loss and phosphatidylserine externalization. Instead, we observed a notable increase in acidic organelle formation, suggesting a pro-survival response in promastigotes. Through diverse flow cytometric analyses and imaging methods, we conclude that the parasitic death induced by these natural compounds does not follow the apoptosis pathway but likely involves autophagy. This discovery marks the first instance of autophagy-mediated cell death in <i>Leishmania donovani</i> triggered by veratramine and hupehenine.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 3","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142739714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special Issue: Fungal Diversity","authors":"Erika Kothe","doi":"10.1002/jobm.202400775","DOIUrl":"10.1002/jobm.202400775","url":null,"abstract":"","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142709078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural Products Produced by the Species of Bacillus cereus Group: Recent Updates","authors":"Ugur Azizoglu,&nbsp;Leandris Argentel-Martínez,&nbsp;Ofelda Peñuelas-Rubio,&nbsp;Angélica Herrera-Sepúlveda,&nbsp;Jerald Conrad Ibal,&nbsp;Reza Sharafi,&nbsp;Gholamreza Salehi Jouzani,&nbsp;Aurelio Ortiz,&nbsp;Jessica Vaca,&nbsp;Estibaliz Sansinenea","doi":"10.1002/jobm.202400666","DOIUrl":"10.1002/jobm.202400666","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Bacillus cereus</i> group produces diverse antimicrobial compounds through different metabolic pathways, including amino acid-based compounds, sugar derivatives, volatile and miscellaneous compounds. These antimicrobial compounds exhibit antibacterial and antifungal activities against various plant pathogens, promoting plant growth and enhancing tolerance to abiotic stresses. They also exhibit nematicidal activities against plant nematodes and antagonistic effects against pathogens in aquatic animals, promoting growth and inducing immune responses. Moreover, <i>B. cereus</i> group bacteria play a significant role in bioremediation by breaking down or neutralizing environmental pollutants, such as plastics, petroleum products, heavy metals, and insecticides. They produce enzymes like laccases, lipases, proteases, and various oxidases, contributing to the degradation of these pollutants. In the food industry, they can cause food poisoning due to their production of enterotoxins. However, they are also utilized in various industrial applications, such as producing environmentally friendly bio-based materials, biofertilizers, and nanoparticles. Notably, <i>B. cereus</i> transforms selenite into selenium nanoparticles, which have health benefits, including cancer prevention. In summary, <i>B. cereus</i> group bacteria have diverse applications in agriculture, bioremediation, industry, and medicine, contributing to sustainable and eco-friendly solutions across multiple fields. In this review, we have revised <i>B. cereus</i> group and the characteristics of every species; we have also highlighted the more important compounds secreted by the species of <i>B. cereus</i> group and the applications of these compounds. The aim is to explain the available secondary metabolites to classify the species from this group, increasing the knowledge about taxonomy of this group.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 3","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142681578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel Cold-Adapted Catechol 1,2-Dioxygenase From Antarctic Sea-Ice Bacterium Halomonas sp. ANT108: Characterization and Immobilization","authors":"Quanfu Wang,&nbsp;Xiaoxuan Zhang,&nbsp;Anqi Wang,&nbsp;Ailin Zhang,&nbsp;Yatong Wang,&nbsp;Yanhua Hou","doi":"10.1002/jobm.202400500","DOIUrl":"10.1002/jobm.202400500","url":null,"abstract":"<div>\u0000 \u0000 <p>The enzyme catechol 1,2-dioxygenase (CAT) plays a critical role in the biosynthesis pathway of <i>cis, cis</i>-muconic acid (CCMA), which serves as an indispensable raw material for various industrial applications. In this research, we cloned a novel cold-adapted CAT (HaCAT) from the Antarctic sea ice bacterium <i>Halomonas</i> sp. ANT108. Homology modeling analysis revealed that HaCAT possessed the characteristic Fe³⁺ binding site and catalytic active site of typical CATs, and it exhibited unique structural adaptations to cold environments. The optimal temperature and pH for recombinant HaCAT (rHaCAT) were found to be 25°C and 6.5, respectively. At 0°C, the enzyme retained a maximum activity of 43.6%, and in the presence of 1.0 M NaCl, its activity reached 173.9%, demonstrating significant salt tolerance. Additionally, the <i>V</i>_max and <i>K</i>_m of rHaCAT were 6.68 μmol/min/mg and 128.90 μM at 25°C, respectively. Furthermore, rHaCAT was successfully immobilized in the metal-organic framework ZIF-8 and retained almost 50% of its activity after five reuse cycles, demonstrating excellent reusability. Overall, these results provided a new resource and theoretical foundation for the industrial biocatalytic production and modification of CAT.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 2","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plant Growth-Promoting Bacteria Associated With Some Salt-Tolerant Plants","authors":"Fatemeh Beitsayahi,&nbsp;Naeimeh Enayatizamir,&nbsp;Leila Nejadsadeghi,&nbsp;Fatemeh Nasernakhaei","doi":"10.1002/jobm.202400446","DOIUrl":"10.1002/jobm.202400446","url":null,"abstract":"<div>\u0000 \u0000 <p>Given the benefits of bacteria associated with the rhizosphere and phytoplane of halophytes, this research focused on examining the plant growth-promoting characteristics of bacteria isolated from <i>Cressa cretica</i>, <i>Suaeda aegyptiaca</i>, and <i>Alhagi graecorum</i>. From the 33 isolates tested, 9 exhibited plant growth-promoting traits. <i>Bacillus rugosus</i> strain CS5 and <i>Bacillus</i> sp. strain SS4 exhibited the notable growth inhibition of the pathogenic fungus <i>Fusarium oxysporum</i>, with values of 47% and 45%, respectively. <i>Bacillus</i> sp. strains SS4 and CS1 demonstrated impressive results in solubilizing phosphorus and zinc, respectively, achieving concentrations of 259 and 271 mg L⁻¹. Additionally, <i>Staphylococcus xylosus</i> strain SR2, <i>Bacillus</i> sp. strain SS4, and <i>Bacillus paralicheniformis</i> strain CR1 thrived in nitrogen-free media. The <i>Priestia filamentosa</i> strain AL4 showed the greatest HCN production, whereas <i>B. paralicheniformis</i> strain CR1 was notable for higher auxin production. The <i>Bacillus</i> sp. strains SS4 and CS1 exhibited greater tolerance than other isolates in a medium containing 600 mM of NaCl. Additionally, inoculating these isolates into soil significantly alleviated the salinity and drought stress on <i>Zea mays</i> seedlings. These findings suggest that further investigation into these strains as microbial inoculants could be beneficial for mitigating salt and drought stress in plants.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 2","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142647787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Roseomonas aestuarii, as a Potential In Situ Surfactin Producer During Hydrocarbon Biodegradation","authors":"Saman Hosseini,&nbsp;Rouhallah Sharifi,&nbsp;Alireza Habibi,&nbsp;Sholeh Khezri","doi":"10.1002/jobm.202400538","DOIUrl":"10.1002/jobm.202400538","url":null,"abstract":"<div>\u0000 \u0000 <p>In situ biosurfactant production by hydrocarbon degrader microorganisms is an attractive approach in the bioremediation of oil contamination because of their compatibility, biodegradability, environmental safety, and stability under extreme environmental conditions. Given the high efficiency of bacteria in degrading petroleum hydrocarbons, the present work studied the detection and characterization of a biosurfactant-producing hydrocarbon degrader, <i>Roseomonas aestuarii</i> NB833. This strain was able to synthesize a biosurfactant during the biodegradation of crude oil, which reduced the surface tension of the aqueous system from 70 to 34 mN m⁻¹, with a critical micelle concentration of 200 mg L⁻¹. The emulsification ability of the biosurfactant was sustained at various temperatures, pH values, and salinities. The biosurfactant chemical structure was identified via FT-IR, LC-MS, and NMR analyses. These analyses confirmed the production of surfactin-C14 with a molecular mass of 1007 g mol⁻¹. These results revealed the high potential of <i>R. aestuarii</i> NB833 as an in situ surfactin-producing bacteria for bioremediation applications under extreme environmental conditions.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 2","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142621088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}