Journal of Basic Microbiology最新文献

Identifying Potent Oxalate-Degrading LAB Strains for the Development of Low Oxalate Rhubarb-Based Juice Products. 低草酸大黄果汁产品的高效草酸降解菌株的鉴定。

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-05-01 DOI: 10.1002/jobm.70171

Pardis Vosoughi, Mohammad B Habibi Najafi, Kianoush Khosravi-Darani, Paria Rahnama-Vosough

{"title":"Identifying Potent Oxalate-Degrading LAB Strains for the Development of Low Oxalate Rhubarb-Based Juice Products.","authors":"Pardis Vosoughi, Mohammad B Habibi Najafi, Kianoush Khosravi-Darani, Paria Rahnama-Vosough","doi":"10.1002/jobm.70171","DOIUrl":"https://doi.org/10.1002/jobm.70171","url":null,"abstract":"Rhubarb, a popular culinary ingredient, is notably high in oxalates, which can contribute to the formation of kidney stones, particularly in individuals susceptible to hyperoxaluria. In light of emerging evidence showcasing the beneficial properties of lactic acid bacteria in dietary oxalate degradation, this study endeavors to identify specific LAB strains capable of efficaciously reducing oxalate levels in rhubarb-based juice. A total of 34 LAB strains, isolated from traditional dairy products, were screened for oxalate-degrading potential. The strains characterized included Lactiplantibacillus plantarum, Levilactobacillus brevis, Enterococcus faecalis, Enterococcus faecium, and Streptococcus thermophilus. Employing titration, an enzymatic oxalate assay, and HPLC, we evaluated the oxalate degradation capabilities of each isolate. Notably, Levilactobacillus brevis (M8) emerged as the most effective strain, exhibiting remarkable oxalate-degrading activity. Subsequently, the strain was utilized in the fermentation of rhubarb-based juice, leading to an optimized fermentation process that involved varying sugar substrates and incubation periods. Fermentation with Levilactobacillus brevis (M8) over a 96-h period resulted in a significant 17.5% reduction in oxalate content, decreasing the concentration from 10.44 mmol/L in the control to 8.50 mmol/L in the optimized fermented juice, while maintaining desirable sensory attributes. The findings underpin the promising application of Levilactobacillus brevis (M8) as an adjunct in the formulation of functional, low-oxalate beverages for at-risk populations.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 5","pages":"e70171"},"PeriodicalIF":2.7,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147814874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early Stage of Biotin Biosynthesis Pathway: Non-Conserved Yet Unique 生物素生物合成途径的早期阶段：非保守但独特。

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-04-06 DOI: 10.1002/jobm.70166

Swati Singh

引用次数: 0

A Rice Rhizosphere-Associated Bacillus sp. With Dual Biosorption and Enzymatic Pathways for Chromium (VI) Detoxification. 具有双生物吸附和酶解毒途径的水稻根际相关芽孢杆菌。

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-04-01 DOI: 10.1002/jobm.70170

Arup Kumar Sarkar, Soumita Roy, Nilakshi Chakraborty, Abir Das, Ribhu Maity, Tithi Maity, Bidhan Chandra Samanta, Tatiana Minkina, Vishnu D Rajput, Malay Kumar Adak

{"title":"A Rice Rhizosphere-Associated Bacillus sp. With Dual Biosorption and Enzymatic Pathways for Chromium (VI) Detoxification.","authors":"Arup Kumar Sarkar, Soumita Roy, Nilakshi Chakraborty, Abir Das, Ribhu Maity, Tithi Maity, Bidhan Chandra Samanta, Tatiana Minkina, Vishnu D Rajput, Malay Kumar Adak","doi":"10.1002/jobm.70170","DOIUrl":"https://doi.org/10.1002/jobm.70170","url":null,"abstract":"Hexavalent Cr(VI) is well known hazardous pollutant which not only impairs the soil health but also drastically reduces the plant developmental processes. Here, we observed a plant growth promoting rhizobacterium (Bacillus sp. AKS_bp1) isolated from rice rhizosphere modulating dual roles in Cr(VI) detoxification and plant responses. Initially, Bacillus sp. (AKS_bp1) showing PGPR characteristic along with a high tolerance to varying Cr(VI) concentrations in laboratory condition. Moreover, the strain also reduce the Cr(VI) concentration (~80%) with a bio-sorption capacity reaching 95.21% under 72 h incubation followed by pseudo first order kinetics. Further, biosorption studies through infrared spectroscopy and scanning micrograph showing the presence of hydroxyl, polysaccharides, and amino-nitrogen based functional groups for Cr binding. Likewise, internal Cr(VI) bio-removal the activity of Cr reductase was recorded with changes through 20, 40, 60, and 80 ppm of Cr(VI) in solution. Enzyme activity was validated with varying pH (5-8), temperature (21°C-45°C) and metal (Ag2+), salt (sodium dodecyl sulphate) etc. In detoxification pathways, the strain recorded further upregulation of antioxidative enzymes highlighting a well-established defence against Cr induced toxicity. Remarkably, application the strain was able to remove 36.86% of Cr(VI) when incubated in tannery effluents through 72 h. Conclusively, the present work provides importance of Bacillus sp. (AKS_bp1) mediated bio-removal of contaminated industrial effluents, which predominantly rich in Cr(VI). This bacterial strain also significantly reduced lipid peroxidation and polyphenol oxidation. Moreover, it offers potential for additional chemical modifications and sustainable bioremediation of Cr and other toxic metals, thereby improving crop growth and productivity.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 4","pages":"e70170"},"PeriodicalIF":2.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147772367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Multifunctional Traits of Trichoderma afroharzianum ISO-02 and Trichoderma brevicrassum ISO-03 Promoting Wheat Growth and Germination. 促进小麦生长和萌发的非洲木霉ISO-02和短革木霉ISO-03的多功能性状

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-04-01 DOI: 10.1002/jobm.70169

Kashifa Noor, Ibrar Muhammad Khan, Misbah Nazeer, Muhammad Jamil, Muhammad Anees

{"title":"Multifunctional Traits of Trichoderma afroharzianum ISO-02 and Trichoderma brevicrassum ISO-03 Promoting Wheat Growth and Germination.","authors":"Kashifa Noor, Ibrar Muhammad Khan, Misbah Nazeer, Muhammad Jamil, Muhammad Anees","doi":"10.1002/jobm.70169","DOIUrl":"https://doi.org/10.1002/jobm.70169","url":null,"abstract":"Wheat as a major staple crop faces productivity constraints, especially in rain-fed and arid areas of Pakistan. Ecofriendly alternatives using indigenous plant growth promoting (PGP) microorganisms may help enhance crop performance. This study evaluated PGP traits of indigenous strains of two under-characterized Trichoderma species, that is, Trichoderma afroharzianum ISO-02 and Trichoderma brevicrassum ISO-03, using in vitro and in planta assays with cell-free culture filtrates (CFCF) or spore-based formulations. To the best of our knowledge, this is the first comparative study evaluating CFCF and spore-based formulations of strains of T. afroharzianum and T. brevicrassum on wheat growth. Briefly, ISO-02 produced higher levels of IAA (9.91 µg/mL) and ammonia (11.63 µg/mL) than ISO-03. Moreover, ISO-02 exhibited significantly higher phosphate (1.15 ± 0.01 mm) and zinc-solubilization indices (1.46 ± 0.03 mm), and produced pectinolytic enzymes and siderophores. The CFCF, or spore suspensions of ISO-02, both in plates and pots, produced significantly higher germination, with increased chlorophyll contents and growth parameters on a consistent basis at p ≤ 0.05. The results also suggested a role of extracellular metabolites in growth stimulation of wheat. The present study demonstrated multifunctional PGP traits of T. afroharzianum ISO-02 and T. brevicrassum ISO-03, supporting their potential application as sustainable biofertilizers specifically for wheat cultivation.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 4","pages":"e70169"},"PeriodicalIF":2.7,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147690226","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Journal of Basic Microbiology. 4/2026 期刊信息：Journal of Basic Microbiology. 4/2026

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-03-30 DOI: 10.1002/jobm.70168

引用次数: 0

Brucella spp. – Emerging Insights Into Virulence Strategies: T4SS, CβG, Intracellular Replication, and Beyond 布鲁氏菌-对毒力策略的新见解：T4SS， CβG，细胞内复制及其他。

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-03-30 DOI: 10.1002/jobm.70167

Kokila Manickam, Suganya Kannan

{"title":"Brucella spp. – Emerging Insights Into Virulence Strategies: T4SS, CβG, Intracellular Replication, and Beyond","authors":"Kokila Manickam, Suganya Kannan","doi":"10.1002/jobm.70167","DOIUrl":"10.1002/jobm.70167","url":null,"abstract":"<div>\u0000 \u0000 Brucellosis, caused by Brucella species, is a major zoonotic disease with substantial public health and veterinary impact worldwide. This review provides an integrated overview of key virulence mechanisms employed by Brucella, with emphasis on the VirB Type IV secretion system (T4SS), cyclic β−1,2-glucans (CβG), lipopolysaccharide (LPS) and the bacterium's intracellular replication cycle. These factors enable the pathogen to evade host immune recognition, remodel intracellular compartments, and maintain long-term survival in diverse tissues. Building on recent advances, we propose a functional hierarchy of VirB T4SS effectors that distinguishes core components required for intracellular persistence from context-dependent effectors that modulate tissue specificity and disease severity. By linking VirB T4SS activity with CβG-driven alterations of phagosomal trafficking and LPS-mediated dampening of Toll-like receptor 4 signaling, we outline a multilayered model of immune escape and chronic infection. On this basis, we identify non-redundant T4SS effectors, CβG biosynthetic enzymes and defined LPS modifications as priority candidates for the development of targeted diagnostics, host-directed or anti-virulence therapies, and next-generation vaccines against brucellosis.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 4","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147581234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Comparative Genomic Analyses of IncPa1763-Kpc Plasmids” 更正“IncPa1763-Kpc质粒的比较基因组分析”。

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-03-23 DOI: 10.1002/jobm.70165

引用次数: 0

Coprinellus sp. DJL-31 Promotes Both Seed Germination and Pseudobulb Growth in Cremastra appendiculata Coprinellus sp. DJL-31对附属草种子萌发和假球茎生长的促进作用。

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-03-23 DOI: 10.1002/jobm.70164

Li-Juan Wu, Tao Xu, Mi Zhang, Yu-Xiao Ling, Wei Wang, Li Feng, Qun Zhao, Bang-Xing Han, Shan-Yong Yi

{"title":"Coprinellus sp. DJL-31 Promotes Both Seed Germination and Pseudobulb Growth in Cremastra appendiculata","authors":"Li-Juan Wu, Tao Xu, Mi Zhang, Yu-Xiao Ling, Wei Wang, Li Feng, Qun Zhao, Bang-Xing Han, Shan-Yong Yi","doi":"10.1002/jobm.70164","DOIUrl":"10.1002/jobm.70164","url":null,"abstract":"<div>\u0000 \u0000 \u0000 Cremastra appendiculata is an endangered medicinal orchid with limited propagation, as its seeds depend on specific symbiotic fungi for germination, and its pseudobulbs have poor propagation efficiency. Pseudobulb-associated endophytes render ex situ cultivation of C. appendiculata more feasible than seed-associated mycorrhizal fungi, as the former are easier to culture under controlled conditions and thus better support the orchid's ex situ propagation. In this study, 35 fungal strains representing 15 genera from 12 families were isolated from C. appendiculata pseudobulbs. Six of these strains were evaluated for growth-promoting capacity, and assays on wheat and sorghum indicated five isolates demonstrated growth-promoting effects on model plants. When their specific symbiotic roles in C. appendiculata were examined, only Coprinellus sp. DJL-31 enhanced seed germination and pseudobulb development. With DJL-31, seed germination was ~85%, and protocorms formed within 45 days. DJL-31 advanced pseudobulb sprouting by 7–10 days and increased sprout number. Microscopy verified that DJL-31 colonized epidermal and cortical cells in protocorm, forming hyphal aggregates. The other four strains that promoted the growth of model crops had no significant effects on C. appendiculata. This is the first to reveal the growth-promoting potential of Coprinellus sp. DJL-31 during the “seed germination–pseudobulb growth” phase of C. appendiculata. These results provide a novel pseudobulb-based screening framework to support the ex situ propagation and conservation of C. appendiculata.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147498772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural Evidence of Chlamydospore Formation in Exophiala dermatitidis Under Environmental Stress 环境胁迫下表皮皮炎衣孢子形成的超微结构证据。

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-03-18 DOI: 10.1002/jobm.70163

Mehalene Jayaram, Hideyuki Nagao

{"title":"Ultrastructural Evidence of Chlamydospore Formation in Exophiala dermatitidis Under Environmental Stress","authors":"Mehalene Jayaram, Hideyuki Nagao","doi":"10.1002/jobm.70163","DOIUrl":"10.1002/jobm.70163","url":null,"abstract":"<div>\u0000 \u0000 Exophiala dermatitidis is an extremotolerant black yeast with clinical significance and environmental resilience, which exhibits dimorphic growth between yeast-like and hyphal forms. Chlamydospore formation, however, has not been reported in this species. This study investigated the structural modification in E. dermatitidis under hydrocarbon-rich conditions and prolonged incubation at 37°C. Exophiala dermatitidis was cultured on Sabouraud dextrose agar (SDA), rose bengal potato dextrose agar chloramphenicol (R-PDA) and in soil extract solution with engine oil (SE). To explore survival mechanisms and promote hyphal development, cultures were maintained for extended periods on R-PDA and potato flakes agar (PFA). Morphological and ultrastructural changes were examined using transmission electron microscopy. Cell wall thickness was measured and analyzed. Thin-walled yeast cells transformed into thick-walled yeast cells on R-PDA and SE. After prolonged incubation, melanized, lipid-rich chlamydospores were observed at hyphal tips and between hyphae. Growth on PFA confirmed chlamydospores development through the conversion into hyphal structures. This study presents the first ultrastructural evidence of chlamydospore formation in E. dermatitidis.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147473528","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Aspergillus conicus Endophyte Improves the Development of Eucalyptus camaldulensis Seedlings In Vitro 圆锥曲霉内生菌促进山梨树离体幼苗发育。

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-03-02 DOI: 10.1002/jobm.70156

Lorrayne Martins da Silva, Danival José de Souza

{"title":"Aspergillus conicus Endophyte Improves the Development of Eucalyptus camaldulensis Seedlings In Vitro","authors":"Lorrayne Martins da Silva, Danival José de Souza","doi":"10.1002/jobm.70156","DOIUrl":"10.1002/jobm.70156","url":null,"abstract":"Fungi of the genus Aspergillus promote plant growth and resistance, enhance nutrient uptake, protect plants against pathogens, and increase tolerance to environmental stress. We examined the symbiosis between Aspergillus conicus and seedlings of Eucalyptus camaldulensis, a forest species widely grown in Brazil for its valuable wood and resilience. The fungus was identified as an endophyte of E. camaldulensis seeds grown in Murashige and Skoog basal medium. We observed that inoculated seedlings developed faster than those without the fungus. In xerophilic medium, A. conicus produced abundant spores. Analysis of the internal transcribed spacer region grouped the isolate with other A. conicus species. Seedlings grown on Murashige and Skoog medium with fungal fragments showed significant shoot growth, more leaves, and greater biomass than uninoculated seedlings. Seeds immersed in A. conicus filtrate for 24 h showed less contamination by other fungi and a higher germination rate than those in the control group. Indole acetic acid production was below the detection limit. The fungus was endophytic, extensively colonizing the roots and present in the stems and leaves of inoculated plants. We investigated the implications of this fungal association with E. camaldulensis seedlings and highlighted its potential benefits for plant growth and development.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 3","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-03-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12954154/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147344334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0