Journal of Basic Microbiology最新文献

{"title":"Correction to \"Understanding Bacterial Resistance to Heavy Metals and Nanoparticles: Mechanisms, Implications, and Challenges\".","authors":"","doi":"10.1002/jobm.70088","DOIUrl":"https://doi.org/10.1002/jobm.70088","url":null,"abstract":"","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70088"},"PeriodicalIF":3.5,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Haloalkaliphilic Archaea as Sources of Carotenoids: Ecological Distribution, Biosynthesis and Therapeutic Applications.","authors":"Anjali Desai, Sanket Ray","doi":"10.1002/jobm.70084","DOIUrl":"https://doi.org/10.1002/jobm.70084","url":null,"abstract":"<p><p>The haloalkaliphilic archaea are a group of unique organisms that can thrive in environments with high salinity and alkaline pH. Theses microorganisms have developed various strategies to survive in polyextereme conditions, carotenoids production included as a one of defense mechanism. The ecological distribution of haloalkaliphilic archaea is in soda lakes. Haloalkaliphilic carotenoids producing archaea includes genera such as Natronococcus, Halostagnicola, Natrialba, Natronobacterium, Natronolimnobius, and Natronorubrum. The main carotenoids produce by haloalkaliphilic archea are bacterioruberin and its derivatives. In silico studies of bacterioruberin and its derivatives have shown potential results in binding to cancer-related proteins like MMP-9, ROS1, Bcl-2 cyclin D1. Bacterioruberin from haloalkophiles have higher antioxidanat potential compare to halophilic archea. Bacterioruberin able to inhibit the most importanat replicative enzyme of viruses. They have gained recent attention due to their antioxidant, anticancer, antiviral and antibacterial properties. The bacterioruberin applied in the field of biotechnological and industrial of these as natural colorants, Immunomodulants, feed additive. Extremozymes of these organisms have advantage due to their ability to active in such extreme condition.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70084"},"PeriodicalIF":3.5,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144698610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Engineered Phages and Engineered and Recombinant Endolysins Against Carbapenem-Resistant Gram-Negative Bacteria: A Focused Review on Novel Antibacterial Strategies.","authors":"Majid Taati Moghadam, Shaghayegh Shahkolahi, Israa A Hashim, Shamsieh Asghari, Ghazale Khodadadi, Fatemeh Moghbeli, Samira Sabzi, Shahla Shahbazi","doi":"10.1002/jobm.70078","DOIUrl":"https://doi.org/10.1002/jobm.70078","url":null,"abstract":"<p><p>Antibiotic resistance has escalated globally, affecting not only commonly used antibiotics but also last-resort agents such as carbapenems and colistin. The rise of antibiotic-resistant bacteria has prompted microbiologists to devise new strategies, with bacteriophages emerging as one of the most promising options. Nevertheless, certain mechanisms have been identified in bacteria that confer resistance to phages. While phage resistance is currently less widespread than antibiotic resistance, challenges such as biofilm formation, newly emerging resistance mechanisms against phages, and the natural limitations of unmodified phages have driven the advancement of engineered phages. This study aims to examine the efficacy of engineered phages and both engineered and recombinant endolysins against carbapenem-resistant Gram-negative bacteria (CR-GNB). We performed a literature review through PubMed, Scopus, Web of Science, and Google Scholar, concentrating on studies that utilized these agents against carbapenem-resistant Gram-negative bacteria (CR-GNB). Reviewed studies indicate potential antibacterial activity of these agents against CR-GNB. By engineering and modifying phages, these agents exhibit improved antimicrobial efficacy, temperature stability, and membrane permeability. Furthermore, they demonstrate the ability to eliminate bacteria with multidrug-resistant (MDR) and extensively drug-resistant (XDR) profiles. These findings suggest the promising potential of engineered phages and endolysins for future clinical applications against CR-GNB.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70078"},"PeriodicalIF":3.5,"publicationDate":"2025-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144690377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"AmpC-Induced Surge in β-Lactam Resistance in Pseudomonas aeruginosa: A Rising Danger.","authors":"Ankumoni Das, Rohit Ruhal","doi":"10.1002/jobm.70082","DOIUrl":"https://doi.org/10.1002/jobm.70082","url":null,"abstract":"<p><p>Multidrug-resistant Pseudomonas aeruginosa poses escalating threats in healthcare settings. This review highlights AmpC β-lactamase's key role in conferring β-lactam resistance. We investigate the regulatory network of ampR, ampD, and ampG genes that control AmpC expression, specifically how mutations cause enzyme overproduction. The study explores AmpC structural characteristics and mutations in conserved regions that improve catalytic performance against newer cephalosporins and carbapenems. The review covers the interaction between penicillin-binding proteins (PBPs) and AmpC β-lactamases, highlighting how PBP alteration affects enzyme production and resistance patterns. To combat resistant P. aeruginosa, we evaluate alternative therapeutic approaches, including collateral sensitivity strategies and phytochemicals as novel antimicrobials or antibiotic adjuvants. This review elucidates the complicated mechanisms that drive AmpC-mediated resistance, providing critical information that is directly applicable to healthcare practice. The findings help to develop personalized therapeutic methods, improve antimicrobial stewardship protocols, and design diagnostic tools for rapid resistance detection. By bridging molecular research to clinical practice, this study explains therapy failures and proposes new intervention techniques, such as phytochemical-enhanced combination therapies and collateral sensitivity methods. This comprehensive understanding promotes the development of precision treatment strategies, ultimately improving patient outcomes and preventing the spread of multidrug-resistant P. aeruginosa in healthcare facilities and communities.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70082"},"PeriodicalIF":3.5,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144674885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomics and Transcriptomics of Candidatus Phytoplasma Asteris Induced Sesame Phyllody Modulating Hormonal and Defense Alterations.","authors":"Raju Karan, Muthukapalli Krishnareddy Prasannakumar, Jayaramareddy Harish, Swathi Shivanagouda Patil, Kurubarahalli Narayanaswamy Pallavi, Gopal Venkateshbabu, Pramesh Devanna, Pushpa Hanumanthappa Deeshappa, Channappa Manjunatha, Aditya Kukreti, Selva Babu Selvamani, Aditya Narayan Sarangi, Raju Soolanayakanahally, Sateesh Kagale","doi":"10.1002/jobm.70080","DOIUrl":"https://doi.org/10.1002/jobm.70080","url":null,"abstract":"<p><p>Sesame phyllody, a destructive disease caused by phytoplasma infection, induces severe morphological abnormalities, including floral virescence, phyllody, witches' broom, leaf deformation, and stunted growth. This study aimed to characterize phytoplasma isolates from diverse regions of India, identifying them as Candidatus Phytoplasma asteris (16Sr-I), Candidatus Phytoplasma citri (16Sr-II), and Candidatus Phytoplasma australasia (16Sr-II). Whole-genome sequencing of Candidatus Phytoplasma asteris isolate SPGN revealed a genome size of 563,754 bp, encoding 542 proteins, including several genes associated with antibiotic resistance. Effector prediction analysis identified key virulence-associated proteins, such as SAP50-like, SAP34-like, TENGU-su inducer, and immunodominant membrane proteins, which manipulate host development and immune responses. Transcriptomic analysis of infected sesame plants revealed significant gene expression alterations, with upregulated genes linked to floral malformation, vascular tissue modifications, and stress responses, while the downregulated genes were associated with flavonoid metabolism and immune signaling. Phytoplasma infection disrupted hormonal pathways, leading to increased expression of auxin, cytokinin, and gibberellin-related genes, suggesting hormonal dysregulation as a key factor in symptom development. Furthermore, immune suppression was evident through the downregulation of key defense-related genes, including those involved in MAPK signaling and pathogenesis-related protein families. These findings enhance our understanding of phytoplasma pathogenesis in sesame and provide potential targets for developing effective disease management strategies.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70080"},"PeriodicalIF":3.5,"publicationDate":"2025-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144649567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Novel In Vitro Host-Pathogen Model for Felis catus and Sporothrix Zoonotic Species Investigation.","authors":"Gabriele Barros Mothé, Nathália Faria Reis, Emylli Dias Virginio, Miguel Angelo da Silva Medeiros, Adriany Lucas Dos Santos, Júlia Andrade de Castro Rodrigues, Ricardo Luiz Dantas Machado, Gutemberg Gomes Alves, Nathália Curty de Andrade, Leila Maria Lopes-Bezerra, Andréa Regina de Souza Baptista","doi":"10.1002/jobm.70077","DOIUrl":"https://doi.org/10.1002/jobm.70077","url":null,"abstract":"<p><p>Hyperendemic zoonotic sporotrichosis, attributed to Sporothrix brasiliensis, presents a significant public health challenge in Brazil. Cats exhibit severe symptoms and high fungal loads, though their susceptibility is unclear. Sporothrix schenckii can also cause feline disease, primarily seen in Asia. This study is the first to report an in vitro model for examining cat immune cell responses to S. brasiliensis or S. schenckii. We investigated the phagocytic activity of blood cells (FMdP) from healthy domestic cats, challenged with yeast cells of S. brasiliensis and S. schenckii. The survival of these yeasts within cat phagocytes and their cytotoxic effect on host cells were monitored. Both fungal species developed and replicated within feline phagocytes while S. brasiliensis phagocytic index (PI) was higher (p < 0.0001). Interspecies analyses showed that S. schenckii required a higher multiplicity of infection to be more cytotoxic than S. brasiliensis (p ≤ 0.01). The present report brings relevant information to understand S. brasiliensis host adaptation and, ultimately, cat susceptibility to sporotrichosis. This pioneering study on the feline's innate immune response provides new insights for future complex studies such as those involving fungal ligand recognition by cat cell receptors.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70077"},"PeriodicalIF":3.5,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144637091","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special Issue: Plant Resilience Through Microbial Biocontrol.","authors":"Erika Kothe","doi":"10.1002/jobm.70079","DOIUrl":"https://doi.org/10.1002/jobm.70079","url":null,"abstract":"","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70079"},"PeriodicalIF":3.5,"publicationDate":"2025-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144583991","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Propagule-Specific Bacteriome of Funneliformis mosseae Spores and Hyphae: Integrated High-Throughput and Culture-Dependent Insights.","authors":"Seema Sangwan, Garima Saxena, Gautam Chawla, Radha Prasanna, Ram Swaroop Bana, Anil K Choudhary","doi":"10.1002/jobm.70076","DOIUrl":"https://doi.org/10.1002/jobm.70076","url":null,"abstract":"<p><p>Arbuscular mycorrhizal (AM) symbiosis is increasingly recognized as a tripartite interaction involving the fungal symbiont, the host plant, and a diverse assemblage of associated bacteria. Through this study, propagule-specific bacteriome of Funneliformis mosseae was explored, particularly its taxonomic composition and plant growth-promoting (PGP) potential. Using a polyphasic approach integrating Illumina high-throughput sequencing with culture-dependent techniques, bacterial communities associated with monosporal hyphae and spores were characterized. Sequencing analyses revealed distinct taxonomic profiles between two propagule types: spores were dominated by Pseudomonas, whereas hyphae harbored higher relative abundances of Sphingobium and Rhodococcus. Culture-dependent screening on NBRIP medium yielded 53 phosphate-solubilizing bacterial isolates-21 from spores and 32 from hyphae. While hyphae-associated propagules contained a greater number of phosphate-solubilizing isolates, those from spores exhibited significantly higher solubilization capacities, ranging from 16.87 to 273 µg mL⁻¹, with 47.6% exceeding 100 µg mL⁻¹. In contrast, hyphae-derived isolates ranged from 35.03 to 142.20 µg mL⁻¹, with 28.1% surpassing the 100-µg mL⁻¹ threshold. Functional screening further revealed that 38% of spore and 31% of hyphae-associated isolates exhibited diverse PGP traits. The five most potent strains were identified through 16S rDNA sequencing as Pseudomonas aeruginosa, Lactiplantibacillus plantarum, Bacillus haynesii, Bacillus licheniformis, and Enterococcus innesii. This study represents the first attempt to characterize a propagule-specific core bacteriome in Funneliformis mosseae, revealing clear taxonomic and functional divergence between spore and hyphae-associated bacterial communities. These findings highlight the specialized ecological roles of distinct propagule microbiomes and offer novel avenues for targeted manipulation of AM symbiosis to enhance plant nutrient acquisition and growth.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70076"},"PeriodicalIF":3.5,"publicationDate":"2025-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144575470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Storage Impact on Chlamydospore Germination of Arthrobotrys flagrans in Sodium Alginate Pellets","authors":"You-lei Li,&nbsp;Bo-bo Wang,&nbsp;Yan-feng Wang,&nbsp;Xuan-yu Chen,&nbsp;Feng-hui Wang,&nbsp;Xin Li,&nbsp;Kui-zheng Cai,&nbsp;Shuang-yan Lv","doi":"10.1002/jobm.70043","DOIUrl":"https://doi.org/10.1002/jobm.70043","url":null,"abstract":"<div>\u0000 \u0000 <p>This study aims to determine the shelf life of four batches of sodium alginate pellets containing the <i>Arthrobotrys flagrans</i> chlamydospores different storage conditions. Results showed that the germination rates of the chlamydospores in batches A and B increased in varying degrees at the indoor storage for 1 month but until 17 months of storage gradually declined to 0.52% and 0.48%, respectively. The germination rates in batches C and D gradually dropped to a minimum value at room temperature for 3–6 months but increased to the maximum value by 12 months and then again decreased to 1.26% and 2.22%, respectively, by 16 months. These pellets stored indoors were transferred to 4°C and −20°C storage. After storage for 2–5 months, the spore germination rates increased in different degrees. After the batch A and B pellets were stored at 4°C for 6, 12, and 30 months each, the chlamydospore germination rates increased to 10.76%, 11.87%, and 15.71%, respectively, for batch A and 29.63%, 8.34%, and 19.89%, respectively, for batch B. After the batch C and D pellet formulations were stored at −20°C for 1 year, the spore germination rates became twice as high as the initial values but close to their initial values at storage for 27 months. The data obtained in this study indicated that the shelf life of the pellets can be maintained for more than 2 years whether stored at 4°C or −20°C, between which the storage at −20°C was better.</p></div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Information: Journal of Basic Microbiology. 7/2025","authors":"","doi":"10.1002/jobm.70068","DOIUrl":"https://doi.org/10.1002/jobm.70068","url":null,"abstract":"","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.70068","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}