Journal of Basic Microbiology最新文献

The Function of TER94 of Spodoptera frugiperda 9 Cells When Infected With Invertebrate Iridescent Virus-6. 无脊椎动物虹彩病毒-6感染夜蛾9细胞TER94的功能

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-05-13 DOI: 10.1002/jobm.70045

Kubra Zengin, Cihan Inan, Remziye Nalcacioglu, Zihni Demirbag

{"title":"The Function of TER94 of Spodoptera frugiperda 9 Cells When Infected With Invertebrate Iridescent Virus-6.","authors":"Kubra Zengin, Cihan Inan, Remziye Nalcacioglu, Zihni Demirbag","doi":"10.1002/jobm.70045","DOIUrl":"https://doi.org/10.1002/jobm.70045","url":null,"abstract":"The 118 L protein in the envelope of the Invertebrate iridescent virus 6 (IIV6) is responsible for binding to receptors on the surface of permissive cells. We previously elucidated its function by silencing its gene and neutralizing the protein with antibodies. In this study, we aimed to identify the cellular protein that interacts with 118 L protein during virus infection. Membrane proteins from Spodoptera frugiperda 9 (Sf9) cells were separated by SDS-PAGE and electro-transferred to a nitrocellulose membrane. Using a virus overlay protein binding assay (VOPBA), the 118 L protein purified by His-tag was shown to interact with a cellular protein larger than 100 kDa. Analysis of the cellular protein by LC-MS/MS revealed that the transitional endoplasmic reticulum ATPase (TER94) was the highest-scoring protein. Docking analysis using the HADDOCK2.4 program confirmed the interaction of 118 L with TER94. Furthermore, a pull-down experiment was performed between the TER94 produced by His-tag in the baculovirus expression system, and the 118 L protein produced by GST-tag in the bacterial expression system. The interaction between these two proteins was visualized by western blot analysis using a monoclonal anti-GST antibody. These results indicate that TER94 is a binding protein for 118 L and plays a significant role in the entry of IIV6 into Sf9 cells.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70045"},"PeriodicalIF":3.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Critical Roles of Conserved Glu 21 and Asp 23 of a Staphylococcal Anti-Anti-Sigma Factor. 保守的Glu 21和Asp 23在葡萄球菌抗-抗- sigma因子中的关键作用。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-05-13 DOI: 10.1002/jobm.70046

Debasmita Sinha, Pritam Naskar, Partha Pratim Sikdar, Tushar Chakraborty, Ajit Bikram Datta, Subrata Sau

{"title":"The Critical Roles of Conserved Glu 21 and Asp 23 of a Staphylococcal Anti-Anti-Sigma Factor.","authors":"Debasmita Sinha, Pritam Naskar, Partha Pratim Sikdar, Tushar Chakraborty, Ajit Bikram Datta, Subrata Sau","doi":"10.1002/jobm.70046","DOIUrl":"https://doi.org/10.1002/jobm.70046","url":null,"abstract":"Staphylococcus aureus and similar bacteria cope with stressful environments using a set of conserved proteins including σB, an alternative sigma factor. The initiation of transcription by σB is obstructed by RsbW, an anti-sigma factor. RsbW also associates and phosphorylates RsbV, an anti-anti-sigma factor. A modeling study previously suggested that Glu 21, Asp 23, and Tyr 54 of S. aureus RsbV form non-covalent bonds with Arg 23, an indispensable residue of cognate RsbW. Herein, we have noted that Glu 21, and Asp 23 are conserved residues, whereas Tyr 54 is a semi-conserved residue. Additionally, our MD simulation studies indicate that both Glu 21 and Asp 23 may maintain the structure of RsbV. To verify the computational data, two RsbV mutants, created by replacing Glu 21 and Asp 23 with an Ala residue, were elaborately investigated using some in vitro tools. The results reveal that both the above residues are critical for preserving the structure of RsbV. Interestingly, the RsbV mutant harboring Ala at position 23 was very little phosphorylated by RsbW. This mutant, compared to the RsbV mutant carrying Ala at position 21, also showed a weaker interaction with RsbW. The ways Glu 21 and Asp 23 keep various properties of RsbV intact have been discussed at length.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70046"},"PeriodicalIF":3.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144022019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Surfactin Activating KinB to Sense and Utilize Carbon Sources Against Cannibalism in Bacillus amyloliquefaciens. 表面素激活KinB感知和利用碳源对抗解淀粉芽孢杆菌的同类相食。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-05-13 DOI: 10.1002/jobm.70049

Jiahong Wen, Rui Wang, Yuqing Zheng, Shilei Jiang, Heli Shi, Jun Tan, Xiuyun Zhao, Gaofu Qi

{"title":"Surfactin Activating KinB to Sense and Utilize Carbon Sources Against Cannibalism in Bacillus amyloliquefaciens.","authors":"Jiahong Wen, Rui Wang, Yuqing Zheng, Shilei Jiang, Heli Shi, Jun Tan, Xiuyun Zhao, Gaofu Qi","doi":"10.1002/jobm.70049","DOIUrl":"https://doi.org/10.1002/jobm.70049","url":null,"abstract":"Bacillus species are ubiquitous microorganisms with broad applications. The lipopeptide surfactin serves as a signaling molecule to activate histidine kinases, subsequently activating the global regulatory protein Spo0A to modulate quorum sensing like cannibalism in response to specific environmental conditions such as carbon source limitation. Our previous studies revealed that deletion of the surfactin synthesis gene in Bacillus amyloliquefaciens resulted in rapid cell death during the logarithmic growth phase. In this study, by employing gene knockout strains involved in the surfactin signaling pathway, we found that B. amyloliquefaciens primarily employs histidine kinase KinB to perceive surfactin signals, enabling the utilization of environmental carbon sources. Surfactin signal transduction is not exclusively dependent on histidine kinases; cells utilize alternative pathways (e.g., cation-induced Spo0A activation) to regulate carbon source utilization. Adding glucose, NADH, and ATP all alleviated cell death in ΔsrfA cultures. Both the wild-type and ΔsrfA mutant produce polypeptide/protein-like autotoxins during growth, but ΔsrfA produced more toxins earlier. Carbon sources such as glucose inhibit autotoxin production, while surfactin initiates detoxifying against these toxins. The absence of Spo0A results in both the loss of autotoxin production and antitoxin capabilities, indicating that Spo0A regulates both toxin production and detoxification mechanisms. Collectively, we firstly reported that in B. amyloliquefaciens, cells utilize surfactin as a signaling molecule to activate KinB, which senses environmental carbon sources and thereby inhibits rather than promotes the production of autotoxins as seen in Bacillus subtilis. However, similar to B. subtilis, KinB activates Spo0A to combat autotoxins.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70049"},"PeriodicalIF":3.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143991579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring Halophiles for Reclamation of Saline Soils: Biotechnological Interventions for Sustainable Agriculture. 探索嗜盐菌开垦盐碱地：可持续农业的生物技术干预。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-05-13 DOI: 10.1002/jobm.70048

Shiwani Bhardwaj, Anila Badiyal, Shailja Dhiman, Jyoti Bala, Abhishek Walia

{"title":"Exploring Halophiles for Reclamation of Saline Soils: Biotechnological Interventions for Sustainable Agriculture.","authors":"Shiwani Bhardwaj, Anila Badiyal, Shailja Dhiman, Jyoti Bala, Abhishek Walia","doi":"10.1002/jobm.70048","DOIUrl":"https://doi.org/10.1002/jobm.70048","url":null,"abstract":"Soil salinization is a major constraint on agricultural productivity, particularly in arid and semi-arid regions where limited rainfall cannot wash salts from plant root zones. This leads to disruptions in water uptake, ion balance, photosynthesis, respiration, nutrient absorption, hormone regulation and rhizosphere microbiome disturbances in plants. Chemical and biological methods can help mitigate soil salinity, but biological approaches, like using halophytes and salt-tolerant microorganisms, are preferred for environmental sustainability. Halophytes, however, represent only about 1% of flora and are habitat specific, so halophilic plant growth-promoting (PGP) microbes have emerged as a key eco-friendly solution. Halophilic PGP bacteria have shown promise in remediating saline soils, enhancing fertility and boosting crop resilience by inducing salinity tolerance (IST) and promoting plant growth traits. In the era of modern agriculture where chemical inputs are at their peak of application rendering the soil infertile, halophilic PGP bacteria represent a promising, sustainable approach to support food security, aligning with Sustainable Development Goals for zero hunger.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70048"},"PeriodicalIF":3.5,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acanthamoeba castellanii Can Facilitate Plasmid Transfer Between Environmental Pseudomonas spp. 卡斯特棘阿米巴促进环境假单胞菌间质粒转移。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-05-11 DOI: 10.1002/jobm.70051

Maarten J Sarink, Lara Grassi, Aloysius G M Tielens, Annelies Verbon, Margreet C Vos, Wil Goessens, Nikolaos Strepis, Corné H W Klaassen, Jaap J van Hellemond

{"title":"Acanthamoeba castellanii Can Facilitate Plasmid Transfer Between Environmental Pseudomonas spp.","authors":"Maarten J Sarink, Lara Grassi, Aloysius G M Tielens, Annelies Verbon, Margreet C Vos, Wil Goessens, Nikolaos Strepis, Corné H W Klaassen, Jaap J van Hellemond","doi":"10.1002/jobm.70051","DOIUrl":"https://doi.org/10.1002/jobm.70051","url":null,"abstract":"The conditions in which antimicrobial resistance (AMR) genes are transferred in natural environments are poorly understood. Acanthamoeba castellanii (a cosmopolitan environmental amoeba) feeds on bacteria by phagocytosis, which places the consumed bacteria closely together in a food vacuole (phagosome) of the amoeba. This way, amoebae can facilitate genetic exchanges between intra-amoebal bacteria. We studied this phenomenon in the clinically relevant bacteria Pseudomonas oleovorans and Pseudomonas aeruginosa (strain 957). The internalization of both the plasmid donor and recipient bacteria was shown by confocal microscopy. In seven independent experiments, an on average 12-fold increase in transfer of the blaVIM-2 gene between these two Pseudomonas strains was observed in the presence of A. castellanii compared to its absence. Negligible or no plasmid transfer was observed from P. oleovorans to 18 other investigated strains of P. aeruginosa. AMR gene transfer via plasmids between Pseudomonas species is highly strain-dependent and A. castellanii can substantially enhance plasmid transfer. This process of plasmid transfer might also occur between other bacteria and predatory protozoa, such as amoebae that reside in the gut of humans and animals.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70051"},"PeriodicalIF":3.5,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144002166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antibacterial and Antibiofilm Activities of Colistin and Ceftazidime-Avibactam Conjugated Silver Nanoparticles. 黏菌素和头孢他啶-阿维巴坦共轭银纳米颗粒的抗菌和抗生物膜活性。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-05-11 DOI: 10.1002/jobm.70044

Yasemin Cezaroğlu, Mümtaz Cem Şirin, Diğdem Trak, Emel Sesli Çetin, Yasin Arslan, Buket Arıdoğan

{"title":"Antibacterial and Antibiofilm Activities of Colistin and Ceftazidime-Avibactam Conjugated Silver Nanoparticles.","authors":"Yasemin Cezaroğlu, Mümtaz Cem Şirin, Diğdem Trak, Emel Sesli Çetin, Yasin Arslan, Buket Arıdoğan","doi":"10.1002/jobm.70044","DOIUrl":"https://doi.org/10.1002/jobm.70044","url":null,"abstract":"Multidrug-resistant (MDR) Gram-negative bacteria constitute a major global health concern, and the increasing resistance to last-line antimicrobial agents compels the urgent improvement of novel treatment strategies. The present study aims to produce colistin and ceftazidime-avibactam conjugated silver nanoparticles by eco-friendly synthesis method and to evaluate the antimicrobial and antibiofilm activities against MDR Gram-negative bacteria. The physicochemical characteristics of nanoparticles were analyzed through ultraviolet-visible spectrophotometry, high resolution-transmission electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy. Broth microdilution assay was conducted to detect minimum inhibitory concentration (MIC) levels of nanoparticles against MDR bacteria and reference strains. The antibiofilm efficacy was assessed by the crystal violet staining method, and the cytotoxic activity was determined using the MTT assay. The characterization analyses demonstrated spherical morphology (5.6-41.6 nm in diameter) and crystalline structures for the nanoparticles. The MIC levels of antibiotic-conjugated nanoparticles against reference strains and MDR clinical isolates ranged from 0.125 to 4 µg/mL, showing inhibitory concentrations (2-1024 fold) lower than that of free antibiotics (p < 0.05). Nanoparticles exhibited more effective biofilm activity on Pseudomonas aeruginosa PAO1 strain compared to free antibiotics, and showed no substantial inhibitory impact on cell viability, even at increased concentrations. The nanoparticles obtained by the green synthesis method could be promising agents in fighting against MDR Gram-negative bacteria.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70044"},"PeriodicalIF":3.5,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of γ-polyglutamic Acid Produced by Drought Tolerant Bacillus subtilis FSO3 on Soil Moisture Retention. 耐旱枯草芽孢杆菌FSO3产γ-聚谷氨酸对土壤保水的影响

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-05-11 DOI: 10.1002/jobm.70047

Emmanuel Oluwakorede Opadokun, Thanaporn Wichai, Panaya Kotchaplai

{"title":"Effect of γ-polyglutamic Acid Produced by Drought Tolerant Bacillus subtilis FSO3 on Soil Moisture Retention.","authors":"Emmanuel Oluwakorede Opadokun, Thanaporn Wichai, Panaya Kotchaplai","doi":"10.1002/jobm.70047","DOIUrl":"https://doi.org/10.1002/jobm.70047","url":null,"abstract":"Drought stress poses significant risks to agricultural productivity by reducing soil moisture availability. Bacterial polymers, such as γ-polyglutamic acid (γ-PGA), have effectively enhanced soil moisture retention and promoted agricultural resilience. This study aimed to investigate the effects of drought stress on Bacillus subtilis FSO3, a glutamate-independent γ-polyglutamic acid (γ-PGA) producer, and to evaluate the potential of its γ-PGA-rich fermentation medium for soil moisture retention. The strain produced up to 2.24 g/L γ-PGA without external glutamate supplementation. Under polyethylene glycol-6000 (PEG-6000)-induced water-deficit conditions, B. subtilis FSO3 demonstrated moderate drought tolerance, with a 50% reduction in maximum biomass at 20% PEG-6000. Interestingly, γ-PGA yield increased from 1.6 g/L at 5% PEG-6000 to 2.3 g/L at 20%. The γ-PGA-rich fermentation medium, applied at 100 mg γ-PGA/kg of soil sustained soil moisture levels above 60% for approximately 33% longer than the control. These findings highlight the potential of the γ-PGA-rich fermentation medium produced by B. subtilis FSO3 as a promising and cost-effective solution for enhancing soil water retention under drought conditions.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70047"},"PeriodicalIF":3.5,"publicationDate":"2025-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144018183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Storage Impact on Chlamydospore Germination of Arthrobotrys flagrans in Sodium Alginate Pellets. 海藻酸钠微球中贮藏对旗鱼衣孢子萌发的影响。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-05-04 DOI: 10.1002/jobm.202500144

You-Lei Li, Bo-Bo Wang, Yan-Feng Wang, Xuan-Yu Chen, Feng-Hui Wang, Xin Li, Kui-Zheng Cai, Shuang-Yan Lv

{"title":"Storage Impact on Chlamydospore Germination of Arthrobotrys flagrans in Sodium Alginate Pellets.","authors":"You-Lei Li, Bo-Bo Wang, Yan-Feng Wang, Xuan-Yu Chen, Feng-Hui Wang, Xin Li, Kui-Zheng Cai, Shuang-Yan Lv","doi":"10.1002/jobm.202500144","DOIUrl":"https://doi.org/10.1002/jobm.202500144","url":null,"abstract":"This study aims to determine the shelf life of four batches of sodium alginate pellets containing the Arthrobotrys flagrans chlamydospores different storage conditions. Results showed that the germination rates of the chlamydospores in batches A and B increased in varying degrees at the indoor storage for 1 month but until 17 months of storage gradually declined to 0.52% and 0.48%, respectively. The germination rates in batches C and D gradually dropped to a minimum value at room temperature for 3-6 months but increased to the maximum value by 12 months and then again decreased to 1.26% and 2.22%, respectively, by 16 months. These pellets stored indoors were transferred to 4°C and -20°C storage. After storage for 2-5 months, the spore germination rates increased in different degrees. After the batch A and B pellets were stored at 4°C for 6, 12, and 30 months each, the chlamydospore germination rates increased to 10.76%, 11.87%, and 15.71%, respectively, for batch A and 29.63%, 8.34%, and 19.89%, respectively, for batch B. After the batch C and D pellet formulations were stored at -20°C for 1 year, the spore germination rates became twice as high as the initial values but close to their initial values at storage for 27 months. The data obtained in this study indicated that the shelf life of the pellets can be maintained for more than 2 years whether stored at 4°C or -20°C, between which the storage at -20°C was better.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70043"},"PeriodicalIF":3.5,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144019554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Journal of Basic Microbiology. 5/2025 期刊信息：Journal of Basic Microbiology. 5/2025

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-05-02 DOI: 10.1002/jobm.70035

引用次数: 0

Cover: Journal of Basic Microbiology. 5/2025 封面：Journal of Basic Microbiology. 5/2025

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-05-02 DOI: 10.1002/jobm.70034

引用次数: 0