Journal of Basic Microbiology最新文献

Storage Impact on Chlamydospore Germination of Arthrobotrys flagrans in Sodium Alginate Pellets 海藻酸钠微球中贮藏对旗鱼衣孢子萌发的影响

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-07-02 DOI: 10.1002/jobm.70043

You-lei Li, Bo-bo Wang, Yan-feng Wang, Xuan-yu Chen, Feng-hui Wang, Xin Li, Kui-zheng Cai, Shuang-yan Lv

{"title":"Storage Impact on Chlamydospore Germination of Arthrobotrys flagrans in Sodium Alginate Pellets","authors":"You-lei Li, Bo-bo Wang, Yan-feng Wang, Xuan-yu Chen, Feng-hui Wang, Xin Li, Kui-zheng Cai, Shuang-yan Lv","doi":"10.1002/jobm.70043","DOIUrl":"https://doi.org/10.1002/jobm.70043","url":null,"abstract":"<div>\u0000 \u0000 This study aims to determine the shelf life of four batches of sodium alginate pellets containing the Arthrobotrys flagrans chlamydospores different storage conditions. Results showed that the germination rates of the chlamydospores in batches A and B increased in varying degrees at the indoor storage for 1 month but until 17 months of storage gradually declined to 0.52% and 0.48%, respectively. The germination rates in batches C and D gradually dropped to a minimum value at room temperature for 3–6 months but increased to the maximum value by 12 months and then again decreased to 1.26% and 2.22%, respectively, by 16 months. These pellets stored indoors were transferred to 4°C and −20°C storage. After storage for 2–5 months, the spore germination rates increased in different degrees. After the batch A and B pellets were stored at 4°C for 6, 12, and 30 months each, the chlamydospore germination rates increased to 10.76%, 11.87%, and 15.71%, respectively, for batch A and 29.63%, 8.34%, and 19.89%, respectively, for batch B. After the batch C and D pellet formulations were stored at −20°C for 1 year, the spore germination rates became twice as high as the initial values but close to their initial values at storage for 27 months. The data obtained in this study indicated that the shelf life of the pellets can be maintained for more than 2 years whether stored at 4°C or −20°C, between which the storage at −20°C was better.</div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 7","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Journal of Basic Microbiology. 7/2025 期刊信息：Journal of Basic Microbiology. 7/2025

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-07-02 DOI: 10.1002/jobm.70068

引用次数: 0

Cover: Journal of Basic Microbiology. 7/2025 封面：Journal of Basic Microbiology. 7/2025

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-07-02 DOI: 10.1002/jobm.70069

引用次数: 0

Emerging Therapeutic Strategies to Combat Antimicrobial Resistance in the Post-Antibiotic Era. 后抗生素时代对抗抗菌素耐药性的新治疗策略。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-07-01 DOI: 10.1002/jobm.70070

Roghayeh Mohammadzadeh, Shahla Shahbazi, Niloufar Khodaei, Samira Sabzi

{"title":"Emerging Therapeutic Strategies to Combat Antimicrobial Resistance in the Post-Antibiotic Era.","authors":"Roghayeh Mohammadzadeh, Shahla Shahbazi, Niloufar Khodaei, Samira Sabzi","doi":"10.1002/jobm.70070","DOIUrl":"https://doi.org/10.1002/jobm.70070","url":null,"abstract":"Antimicrobial resistance (AMR) is a serious global health issue. This review aims to explore alternative therapeutic strategies for combating AMR. The goal is to evaluate emerging treatments that target resistant pathogens through novel mechanisms, bypassing the limitations of traditional antibiotics. Recent researches highlight several promising alternatives, including antibodies, antimicrobial peptides, bacteriocins, bacteriophages, and probiotics (in the clinical trials) and synthetic antimicrobial peptides, anti-virulence strategies, genetically modified phages, antibacterial oligonucleotides, CRISPR-Cas9, and predatory bacteria (in the research stage). These therapies demonstrate potential to overcome AMR by targeting specific bacterial mechanisms, reducing toxicity, and evading resistance. Alternative therapies for AMR present significant promise, offering new avenues for treatment. Despite challenges in optimization and delivery, these therapies could revolutionize the way bacterial infections are treated. Continued research is crucial to address hurdles and ensure these therapies can be safely and effectively implemented in clinical settings, shaping the future of infection management.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70070"},"PeriodicalIF":3.5,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144540335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influence of AHL and Imipenem on bla_NDM Conjugation and sRNA Rydb Expression in Escherichia coli. AHL和亚胺培南对大肠杆菌blaNDM结合及sRNA Rydb表达的影响

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-06-23 DOI: 10.1002/jobm.70074

Chandrayee Deshamukhya, Bhaskar Jyoti Das, Debadatta Dhar, Amitabha Bhattacharjee

{"title":"Influence of AHL and Imipenem on blaNDM Conjugation and sRNA Rydb Expression in Escherichia coli.","authors":"Chandrayee Deshamukhya, Bhaskar Jyoti Das, Debadatta Dhar, Amitabha Bhattacharjee","doi":"10.1002/jobm.70074","DOIUrl":"https://doi.org/10.1002/jobm.70074","url":null,"abstract":"The rise of carbapenem resistance in Escherichia coli is mainly due to the rapid spread of carbapenemase-encoding genes through horizontal gene transfer, particularly via bacterial conjugation. Recent research has highlighted the role of a small RNA molecule known as RydB in bacterial conjugation, specifically through its interaction with the protein SdiA. This study investigated the effects of sub-inhibitory concentrations of imipenem and N-acyl homoserine lactones (AHLs) on the expression of rydB in E. coli strains that overexpress sdiA. Additionally, we examined how AHLs influence the bacterial conjugation of plasmids that contain carbapenem resistance genes. We selected a carbapenem-resistant isolate of E. coli harbouring the blaNDM gene and its corresponding plasmid-cured derivative, based on the overexpression of the sdiA gene in response to AHLs. Conjugation experiments were conducted, both without AHL treatment and with AHL treatments, to assess the transferability of the blaNDM plasmid. The transcriptional response of rydB gene was evaluated in the plasmid-cured derivative, the native type, the transconjugant, and E. coli J53. Our findings indicated that AHLs and imipenem inhibit the expression of the rydB gene. Interestingly, while RydB does not seem to impact bacterial conjugation when suppressed by these agents, the combination of AHLs enhances the conjugation of plasmid that carry the blaNDM gene. This study enhances our understanding of the regulatory roles that quorum sensing signal molecules, including C4 AHL and C12AHL, as well as imipenem, play in bacterial conjugation and sRNA expression.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70074"},"PeriodicalIF":3.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological and Molecular Responses of Anabaena laxa to Rac-Metalaxyl Fungicide: Exploring Metabolic and Oxidative Changes. 疏水水藻对rac -甲氨酯杀菌剂的生理和分子反应：探讨代谢和氧化变化。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-06-23 DOI: 10.1002/jobm.70073

Wael A Fathy, Emad A Alsherif, Shereen M Korany, Abeer S Aloufi, Samy Selim, Amira M Shaban

{"title":"Physiological and Molecular Responses of Anabaena laxa to Rac-Metalaxyl Fungicide: Exploring Metabolic and Oxidative Changes.","authors":"Wael A Fathy, Emad A Alsherif, Shereen M Korany, Abeer S Aloufi, Samy Selim, Amira M Shaban","doi":"10.1002/jobm.70073","DOIUrl":"https://doi.org/10.1002/jobm.70073","url":null,"abstract":"Rac-metalaxyl is a widely used fungicide for managing plant diseases; however, its environmental persistence and potential toxicity to aquatic microorganisms raise significant ecological concerns. Despite its widespread application, there is limited understanding of how cyanobacteria, which play vital ecological roles in aquatic systems, respond to such chemical stressors. This study addresses this gap by investigating the physiological and metabolic responses of the cyanobacterium Anabaena laxa to rac-metalaxyl exposure. Cultures were treated with 100 mg/L and 200 mg/L concentrations of the fungicide. Results showed increased intracellular accumulation of rac-metalaxyl at 200 mg/L, leading to significant reductions in cell growth, photosynthetic pigments, and the activities of key enzymes such as phosphoenolpyruvate carboxylase and ribulose-1,5-bisphosphate carboxylase/oxygenase. Further elevated lipid peroxidation levels indicated oxidative damage. Consequently, rac-metalaxyl triggered substantial metabolic shifts, total soluble sugars increased by 7.66% at 100 mg/L and 67.48% at 200 mg/L; malic acid rose to 76.23% at 200 mg/L; amino acids increased by 27.14% at 100 mg/L and 48.8% at 200 mg/L; total fatty acids rose by 10.11% at 100 mg/L and 35.06% at 200 mg/L. These findings suggest that Anabaena laxa exhibits coordinated oxidative and metabolic responses to mitigate rac-metalaxyl toxicity, highlighting its potential resilience and role in the bioremediation of contaminated aquatic environments.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70073"},"PeriodicalIF":3.5,"publicationDate":"2025-06-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144475381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Actinobacteria From Bryophytes for Growth Promotion of Tropical Moss Species. 苔藓植物放线菌对热带苔藓生长的促进作用。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-06-19 DOI: 10.1002/jobm.70071

Mathurin Meethangdee, Narin Printarakul, Wasu Pathom-Aree

{"title":"Actinobacteria From Bryophytes for Growth Promotion of Tropical Moss Species.","authors":"Mathurin Meethangdee, Narin Printarakul, Wasu Pathom-Aree","doi":"10.1002/jobm.70071","DOIUrl":"https://doi.org/10.1002/jobm.70071","url":null,"abstract":"Actinobacteria have shown significant potential in promoting the growth of various plant species, especially those that inhabit in association with plants. However, previous research has mainly focused on higher plant species. Bryophytes are a large group of non-vascular, non-flowering land plants without true vascular bundles, roots, stems, and leaves. Despite the extensive diversity of bryophytes in Thailand, research on the diversity of actinobacteria associated with bryophytes remains scarce. This study aimed to isolate actinobacteria from three bryophyte species in northern Thailand: Koponobryum papillosum Printarakul & Chantanaorr, Pseudotrismegistia undulata, and Sphagnum cuspidatulum C. Müll. A total of 52 isolates were identified, including 6 endophytic and 46 epiphytic isolates. These isolates were assigned to four genera: Brevibacterium (1 isolate), Microbacterium (1 isolate), Rhodococcus (6 isolates), and Streptomyces (44 isolates). All isolates were assessed for their plant-growth promoting abilities both in vitro and in planta. Most actinobacteria exhibited the in vitro ability to produce indole-3-acetic acid (IAA), siderophores, and solubilize tricalcium phosphate. Three isolates-Streptomyces brevispora RHP2, S. halstedii WS1, and Rhodococcus triatomae RHK22-were selected to evaluate their potential to enhance the growth of S. cuspidatulum C. Müll in soil. R. triatomae RHK22 was highly effective in promoting the regeneration of new shoots and significantly enhancing thallus height, fresh weight, dry weight, total chlorophyll content, and total carotenoid content (p < 0.05) of S. cuspidatulum C. Müll when applied as cell suspension and cell free supernatant.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70071"},"PeriodicalIF":3.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144325793","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Unveiling the Antibacterial Activity Against Staphylococcus aureus of Slime Molds: The Role of Symbiotic Bacteria. 揭示黏菌对金黄色葡萄球菌的抗菌活性：共生细菌的作用。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-06-19 DOI: 10.1002/jobm.70072

Dong-Sheng Yang, Thuy Trang Tran, Higuchi Kazuki, Hao-Yun Yin, Jui-Yu Chou

{"title":"Unveiling the Antibacterial Activity Against Staphylococcus aureus of Slime Molds: The Role of Symbiotic Bacteria.","authors":"Dong-Sheng Yang, Thuy Trang Tran, Higuchi Kazuki, Hao-Yun Yin, Jui-Yu Chou","doi":"10.1002/jobm.70072","DOIUrl":"10.1002/jobm.70072","url":null,"abstract":"The emergence of multidrug-resistant pathogens has significantly reduced the efficacy of current antimicrobial treatments against bacterial and fungal infections. To combat this challenge, the exploration of novel antimicrobial sources or the development of synthetic antibiotics is imperative. Microbes have emerged as promising natural reservoirs for antimicrobial compounds, with slime molds garnering attention due to their unique bioactive metabolites in recent years. Some of these metabolites demonstrate potent antibiotic properties. This study investigates the inhibitory effects of slime mold extracts on pathogenic bacteria, attributing this activity primarily to symbiotic bacteria associated with the slime molds rather than to the slime mold cells themselves. Furthermore, we demonstrate that this antibacterial effect can be horizontally transferred through bacterial ingestion, enabling recipient slime molds to exhibit antibacterial properties upon extraction. Importantly, slime molds selectively acquire bacteria from their environment to enhance their antibacterial characteristics, a process that appears non-random and persists through sexual cycles. These findings underscore slime molds as valuable reservoirs of antimicrobial agents. Nevertheless, it remains critical to ascertain whether these antimicrobial agents originate solely from symbiotic bacteria or result from complex interactions between these bacteria and their slime mold hosts. Understanding the mechanisms behind this antimicrobial activity not only expands our knowledge of host-microbe interactions but also provides new avenues for bioprospecting novel antibiotics. Investigating how slime molds selectively acquire and retain beneficial bacteria may offer insights into microbial symbiosis that could be leveraged for antimicrobial discovery, potentially addressing the urgent need for alternative treatments against resistant pathogens.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70072"},"PeriodicalIF":3.5,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144333215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotechnological Potential of the Fusarium fujikuroi Complex From Amazonian Oenocarpus bacaba: Lipase Production and Characterization. 亚马孙棕榈镰刀菌复合体的生物技术潜力：脂肪酶的产生和特性。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-06-08 DOI: 10.1002/jobm.70067

Lucely Nogueira Dos Santos, Rafael Firmani Perna, Ana Carolina Vieira, Kalebe Ferreira Furtado, Alex Fernando de Almeida, Cristiane Angélica Ottoni, Leandro Mantovani de Castro, Felipe Mello, Yoannis Domínguez, Nelson Rosa Ferreira

{"title":"Biotechnological Potential of the Fusarium fujikuroi Complex From Amazonian Oenocarpus bacaba: Lipase Production and Characterization.","authors":"Lucely Nogueira Dos Santos, Rafael Firmani Perna, Ana Carolina Vieira, Kalebe Ferreira Furtado, Alex Fernando de Almeida, Cristiane Angélica Ottoni, Leandro Mantovani de Castro, Felipe Mello, Yoannis Domínguez, Nelson Rosa Ferreira","doi":"10.1002/jobm.70067","DOIUrl":"https://doi.org/10.1002/jobm.70067","url":null,"abstract":"This study investigates the diversity of filamentous fungi in the Amazon biome, particularly the genus Fusarium, known for producing biotechnologically valuable metabolites. The research aimed to isolate and identify fungi from Oenocarpus bacaba Mart. fruits and analyze the biochemical properties of lipase produced by the strains (maximum activity of 1750 U mL⁻¹). Two isolates, FF1 and FF2, were identified using morphological and molecular techniques, with ITS sequence data suggesting they belong to the Fusarium fujikuroi species complex, though the exact species remains unconfirmed. This is the first report that highlights the biotechnological potential of the F. fujikuroi complex isolated from Oenocarpus bacaba, emphasizing the relevance of Amazonian biodiversity as a source of microorganisms with promising applications in sustainable industrial processes. The results show that native fruits, such as bacaba, are effective matrices for prospecting filamentous fungi producers of enzymes of biotechnological interest, such as lipases. These findings reinforce the importance of rational exploitation of the Amazonian microbiota for the development of bioproducts and eco-efficient industrial processes.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70067"},"PeriodicalIF":3.5,"publicationDate":"2025-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144247978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Characterization of Newly Discovered Polyester Polyurethane-Degrading Methylobacterium aquaticum Strain A1. 新发现的聚酯聚氨酯降解甲基细菌aquaticum菌株A1的表征。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-06-04 DOI: 10.1002/jobm.70066

Seong Hyeon Lee, Haemin Jeong, Injun Jung, Myounghyun Choi, Ah-Ram Kim

引用次数: 0