Journal of Basic Microbiology最新文献

Cover: Journal of Basic Microbiology. 4/2025

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-04-07 DOI: 10.1002/jobm.70032

引用次数: 0

Issue Information: Journal of Basic Microbiology. 4/2025

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-04-07 DOI: 10.1002/jobm.70031

引用次数: 0

Evaluating the Phytohormone Proficiencies of Multifarious Bacillus rugosus for Growth Promotion in Arachis hypogaea (L.).

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-04-03 DOI: 10.1002/jobm.70011

Aniruddh Rabari, Janki Ruparelia, Chaitanya Kumar Jha, Kahkashan Perveen, Abhijit Debnath, Maheshwari Behra, Andrea Mastinu

{"title":"Evaluating the Phytohormone Proficiencies of Multifarious Bacillus rugosus for Growth Promotion in Arachis hypogaea (L.).","authors":"Aniruddh Rabari, Janki Ruparelia, Chaitanya Kumar Jha, Kahkashan Perveen, Abhijit Debnath, Maheshwari Behra, Andrea Mastinu","doi":"10.1002/jobm.70011","DOIUrl":"https://doi.org/10.1002/jobm.70011","url":null,"abstract":"The application of Plant Growth-Promoting Rhizobacteria (PGPR) in agriculture is increasingly emphasized as a sustainable alternative to hazardous agrochemicals. This study aimed to isolate and characterize PGPR strains from the rhizospheric soil of Arachis hypogaea L., hypothesizing that the rhizosphere of a healthy plant harbors beneficial microbes with significant plant growth-promoting (PGP) attributes. The isolate AB1 demonstrated promising PGP traits, including phosphate solubilization (56.44 µg mL⁻¹), zinc solubilization (6.1 µg mL⁻¹), ammonia production (3.8 µM µg mL⁻¹), and the synthesis of hydrogen cyanide (HCN) and phytohormones. Objectives included identifying these traits and evaluating their impact on the growth of Arachis hypogaea L. Phytohormonal profiling of AB1 through Gas Chromatography-Mass Spectrometry (GC-MS) and Fourier Transform Infrared Spectroscopy (FTIR) confirmed indole fractions with characteristic peaks at 3338 cm⁻¹ (N-H stretching), 1641 cm⁻¹ (C-N bond of the indole ring), and 2984 cm⁻¹ (C-H aromatic stretching). Cytokinins and gibberellins were also detected. Molecular, physiological, and biochemical analyses identified the isolate as Bacillus rugosus AB1, with gene sequences deposited under GenBank accession number MZ373174. The present study is the first report of Bacillus rugosus AB1 as a PGPR, showcasing multifaceted PGP traits that significantly enhanced root and shoot growth, biomass, and chlorophyll content in Arachis hypogaea L., demonstrating its potential as a biofertilizer for sustainable agriculture.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70011"},"PeriodicalIF":3.5,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772407","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Actinobacteria as Proficient Biocontrol Agents for Combating Fungal Diseases in Forest Plant Species.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-04-02 DOI: 10.1002/jobm.70030

Pooja Kapoor, Pooja Joshi, Manoj Pal, Vipin Parkash

{"title":"Actinobacteria as Proficient Biocontrol Agents for Combating Fungal Diseases in Forest Plant Species.","authors":"Pooja Kapoor, Pooja Joshi, Manoj Pal, Vipin Parkash","doi":"10.1002/jobm.70030","DOIUrl":"https://doi.org/10.1002/jobm.70030","url":null,"abstract":"Treating fungal pathogens with biocontrol agents and their bio-products is a cost effective, environmental friendly and suitable alternative strategy for managing fungal phytopathogens, minimizing the need for synthetic chemical fungicides. Actinomycetota/Actinobacteria are globally accepted as a major contributor and potent biological control agents in controlling fungal plant diseases. The objective of this review is to evaluate the promising biocontrol efficacy of Actinomycetota in managing fungal pathogens causing diseases in forest plant species, an area that has received less attention compared to crops. It aims to highlight the importance of Actinomycetota, mechanisms of the biocontrol process against phytopathogens, the commercial applications of Streptomyces spp. as effective biocontrol agents and research trends, emphasizing their role as an environmentally sustainable alternative to synthetic fungicides. Additionally, the study identifies possibilities and provides recommendations for future research on Actinomycetota's potential as proficient biocontrol agents against fungal pathogens in forestry.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70030"},"PeriodicalIF":3.5,"publicationDate":"2025-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143772404","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing Pea Plant Growth, Nutrient Acquisition, and Symbiosis in Cobalt-Stressed Soil Using Metal-Tolerant Klebsiella sp.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-28 DOI: 10.1002/jobm.70025

Mohammad Danish, Mohammad Shahid, Sobhy M Ibrahim, Lukman Ahamad

{"title":"Enhancing Pea Plant Growth, Nutrient Acquisition, and Symbiosis in Cobalt-Stressed Soil Using Metal-Tolerant Klebsiella sp.","authors":"Mohammad Danish, Mohammad Shahid, Sobhy M Ibrahim, Lukman Ahamad","doi":"10.1002/jobm.70025","DOIUrl":"https://doi.org/10.1002/jobm.70025","url":null,"abstract":"Excessive cobalt (Co) levels in agricultural soil cause significant toxicity, reducing crop growth and yield. This study aimed to assess the potential of Klebsiella sp. SRB-5 (Accession no. OR715782), in mitigating cobalt toxicity and enhancing the growth of garden peas under cobalt stress. Strain SRB-5, tolerant to 4000 ppm of Co(II), was evaluated for producing growth-regulating substances, including indole-3-acetic acid (IAA), ammonia, siderophore, ACC deaminase, and solubilized phosphate, under cobalt stress. The optimal conditions for Co-(II) biosorption by SRB-5 were determined to be 25°C, pH 6.0, and an incubation time of 72 h. The strain's ability to mitigate Co-(II) toxicity was tested by inoculating peas grown in soil treated with 1000, 2000, and 3000 ppm Co-(II). Inoculation with Co-tolerant SRB-5 alleviated cobalt toxicity and significantly enhanced the physiological and biochemical properties of plants. Notably, SRB-5 increased root length (19.2%), root biomass (29%), seedling vigor index (18.4%), total chlorophyll (52%), nodule biomass (41%), leghaemoglobin content (38%), root nitrogen (27%), and phosphorous content (19.3%) in 1000 ppm Co-stressed peas. Additionally, bacterial inoculation reduced proline, malondialdehyde (MDA), hydrogen peroxide (H2O2), and membrane injury by 85%, 57.3%, 90%, and 75%, respectively, in 1000 ppm Co-exposed plants. Priming with SRB-5 also reduced cobalt uptake in roots (88%), shoots (53.7%), and grains (79.6%) compared to uninoculated treatments. Metal-tolerant beneficial soil bacteria, such as Klebsiella sp. strain SRB-5, could serve as an effective alternative for enhancing pea production in metal-contaminated soils. The use of Co-tolerant PGPR strains holds potential for development as biofertilizers in future agricultural practices.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70025"},"PeriodicalIF":3.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pseudomonas From Extreme Environments: Physiological and Molecular Adaptations.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-28 DOI: 10.1002/jobm.70028

Leandro Pio de Sousa

引用次数: 0

Special Issue: Gene Expression and Regulation.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-28 DOI: 10.1002/jobm.70029

Erika Kothe

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Molecular Characterization of Bacterial Cellulose Producing Bacillus Strains Isolated From Soil.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-28 DOI: 10.1002/jobm.70026

Idris Bektas, Nazli Betul Yildirim

{"title":"Molecular Characterization of Bacterial Cellulose Producing Bacillus Strains Isolated From Soil.","authors":"Idris Bektas, Nazli Betul Yildirim","doi":"10.1002/jobm.70026","DOIUrl":"https://doi.org/10.1002/jobm.70026","url":null,"abstract":"Bacterial cellulose (BC) a remarkable natural biopolymer, offering diverse applications across various industries. This study aimed to identify bacteria that are capable of producing bacterial cellulose through the utilization of various carbon sources isolated from soil. BC producing strains were identified as Bacillus amyloliquefaciens B1, Bacillus subtilis B2, Bacillus velezensis B3, Bacillus siamensis B4, and B. amyloliquefaciens B5 by 16 s rDNA sequencing analysis. The highest amount of BC production observed was when Sucrose was used as the carbon source, the trend B. siamensis B4 (3.0 ± 0.35 g L-1) > B. subtilis B2 (2.8 ± 0.57 g L-1) > B. velezensis B3 (2.5 ± 0.85 g L-1) > B. amyloliquefaciens B1 (2.3 ± 0.68 g L-1) > B. amyloliquefaciens B5 (2 ± 0.71 g L-1). The antibacterial activity of BCs was examined against Escherichia coli and Staphylococcus aureus. The BC produced by B. amyloliquefaciens B5 showed the highest antibacterial activity against S. aureus and and E. coli by producing a clear inhibition zone of 11 and 9 mm, respectively. The water retention capability (WRC %) of the BCs demonstrated a notable range, measuring between 177.7% and 150%. Additionally, the moisture content (MC %) of these samples varied, falling within a range of 64%-60%. The formation of BCs was detected by FTIR analysis. The BC membrane were exhibited definitive characteristics, including a robust nanodimensional network and microfibrils, as evidenced by scanning electron microscopy (SEM). Furthermore, investigation molecular basis of cellulose synthesis of Bacillus strains during cellulose synthesis could offer valuable insights for optimizing production processes.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70026"},"PeriodicalIF":3.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autoinducer-2 Quorum Sensing Is an Active Universal Signaling System in Sociomicrobiology.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-28 DOI: 10.1002/jobm.70024

Dingqi Liu, Wei Zhang, Li Zhu, Jinghai Gong, Yi Huang, Zhou Li, Jin He

{"title":"Autoinducer-2 Quorum Sensing Is an Active Universal Signaling System in Sociomicrobiology.","authors":"Dingqi Liu, Wei Zhang, Li Zhu, Jinghai Gong, Yi Huang, Zhou Li, Jin He","doi":"10.1002/jobm.70024","DOIUrl":"https://doi.org/10.1002/jobm.70024","url":null,"abstract":"Autoinducer-2 (AI-2) is a bacterial quorum sensing (QS) signaling molecule that regulates inter-specific and intraspecific bacterial communication in complex ecological environments. Here, we systematically analyzed the distribution of AI-2 QS-related proteins (synthases and receptors) in the domain bacteria, explored the evolution and development of AI-2 receptors, and analyzed the AI-2 regulatory networks using human intestinal metagenomic data. The results show that AI-2 QS-related proteins are distributed in 17 bacterial phyla, accounting for approximately 36.80% of the total genomes. Based on the ability of bacteria to synthesize and receive AI-2 signals, we divided bacteria into four major categories, namely Prosumer, Producer, Monitor, and Immunizer. The Gram-positive bacteria are mainly responsible for producing the AI-2 signals, while Gram-negative bacteria are more likely to respond to AI-2. Evolutionary analysis shows that the AI-2 receptor CahR is mainly derived from the methyl-accepting chemotaxis protein (MCP). Based on the length difference of the ligand-binding domains, we further speculate that AI-2 binding activates CahR through either allostery or aggregation mode. Using human intestinal metagenomic data, we found a strong correlation between AI-2 signaling and c-di-GMP signaling. These findings will have an important impact on the AI-2 QS research and accelerate its development.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e024"},"PeriodicalIF":3.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Biocontrol of Mycosphaerella fijiensis by five Species of Rhizosphere Fungi of Plantain.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-23 DOI: 10.1002/jobm.70020

Ousmane Fofana, Rosin Don Rodrigue Voko Bi, Kouassi Clément Kouassi

{"title":"Evaluation of Biocontrol of Mycosphaerella fijiensis by five Species of Rhizosphere Fungi of Plantain.","authors":"Ousmane Fofana, Rosin Don Rodrigue Voko Bi, Kouassi Clément Kouassi","doi":"10.1002/jobm.70020","DOIUrl":"https://doi.org/10.1002/jobm.70020","url":null,"abstract":"This study assessed the efficacy of certain rhizosphere fungi isolated from banana plantations in the Haut-Sassandra region as biocontrol agents against Mycosphaerella fijiensis, responsible for banana black spot. It involved characterizing banana leaf and rhizosphere fungi and studying the antagonistic activity of rhizosphere fungi on M. fijiensis. Analyses identified isolates M9 and M10 as M. fijiensis. Five fungal species were isolated from the rhizosphere: T. ghanense, T. longibrachiatum, T. reesei, Neurospora dictyophora and H. terricola. In vitro tests revealed that these fungi inhibit the growth of M. fijiensis, using antagonistic mechanisms such as competition, antibiosis and mycoparasitism. The strongest antagonistic activity was observed with N. dictyophora. Moreover, the extracts derived from these antagonists showed high antifungal activity, with total inhibition of M. fijiensis from 2.5 mg/mL upwards. The minimum fungicidal concentration (MFC) for all extracts was 5 mg/mL, except for T. ghanense which showed an MFC of 10 mg/mL. These results suggest that the rhizospheric fungi studied are promising candidates for the development of M. fijiensis biocontrol strategies.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70020"},"PeriodicalIF":3.5,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0