Journal of Basic Microbiology最新文献

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Enhancing Pea Plant Growth, Nutrient Acquisition, and Symbiosis in Cobalt-Stressed Soil Using Metal-Tolerant Klebsiella sp.
IF 3.5 4区 生物学
Journal of Basic Microbiology Pub Date : 2025-03-28 DOI: 10.1002/jobm.70025
Mohammad Danish, Mohammad Shahid, Sobhy M Ibrahim, Lukman Ahamad
{"title":"Enhancing Pea Plant Growth, Nutrient Acquisition, and Symbiosis in Cobalt-Stressed Soil Using Metal-Tolerant Klebsiella sp.","authors":"Mohammad Danish, Mohammad Shahid, Sobhy M Ibrahim, Lukman Ahamad","doi":"10.1002/jobm.70025","DOIUrl":"https://doi.org/10.1002/jobm.70025","url":null,"abstract":"<p><p>Excessive cobalt (Co) levels in agricultural soil cause significant toxicity, reducing crop growth and yield. This study aimed to assess the potential of Klebsiella sp. SRB-5 (Accession no. OR715782), in mitigating cobalt toxicity and enhancing the growth of garden peas under cobalt stress. Strain SRB-5, tolerant to 4000 ppm of Co(II), was evaluated for producing growth-regulating substances, including indole-3-acetic acid (IAA), ammonia, siderophore, ACC deaminase, and solubilized phosphate, under cobalt stress. The optimal conditions for Co-(II) biosorption by SRB-5 were determined to be 25°C, pH 6.0, and an incubation time of 72 h. The strain's ability to mitigate Co-(II) toxicity was tested by inoculating peas grown in soil treated with 1000, 2000, and 3000 ppm Co-(II). Inoculation with Co-tolerant SRB-5 alleviated cobalt toxicity and significantly enhanced the physiological and biochemical properties of plants. Notably, SRB-5 increased root length (19.2%), root biomass (29%), seedling vigor index (18.4%), total chlorophyll (52%), nodule biomass (41%), leghaemoglobin content (38%), root nitrogen (27%), and phosphorous content (19.3%) in 1000 ppm Co-stressed peas. Additionally, bacterial inoculation reduced proline, malondialdehyde (MDA), hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>), and membrane injury by 85%, 57.3%, 90%, and 75%, respectively, in 1000 ppm Co-exposed plants. Priming with SRB-5 also reduced cobalt uptake in roots (88%), shoots (53.7%), and grains (79.6%) compared to uninoculated treatments. Metal-tolerant beneficial soil bacteria, such as Klebsiella sp. strain SRB-5, could serve as an effective alternative for enhancing pea production in metal-contaminated soils. The use of Co-tolerant PGPR strains holds potential for development as biofertilizers in future agricultural practices.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70025"},"PeriodicalIF":3.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pseudomonas From Extreme Environments: Physiological and Molecular Adaptations.
IF 3.5 4区 生物学
Journal of Basic Microbiology Pub Date : 2025-03-28 DOI: 10.1002/jobm.70028
Leandro Pio de Sousa
{"title":"Pseudomonas From Extreme Environments: Physiological and Molecular Adaptations.","authors":"Leandro Pio de Sousa","doi":"10.1002/jobm.70028","DOIUrl":"https://doi.org/10.1002/jobm.70028","url":null,"abstract":"<p><p>Extremophiles are defined as organisms that grow under environmental conditions considered extreme by human standards, such as high/low pH, high/low temperature, high radiation, high salinity, high pressures, high desiccation etc. Research on extremophiles has led to several advances in molecular biology and bioengineering, providing new insights about the origin of life on Earth and the possibility of interplanetary life. Although most Pseudomonas are mesophilic, some species can grow in extreme environments. Even so, Pseudomonas with these characteristics still receive little attention. This review seeks precisely to help raise interest in the topic by compiling part of the information about extremophile/tolerant Pseudomonas. Resistance to cold, heat, extreme pH, UV, salinity and desiccation were evaluated.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e028"},"PeriodicalIF":3.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Special Issue: Gene Expression and Regulation.
IF 3.5 4区 生物学
Journal of Basic Microbiology Pub Date : 2025-03-28 DOI: 10.1002/jobm.70029
Erika Kothe
{"title":"Special Issue: Gene Expression and Regulation.","authors":"Erika Kothe","doi":"10.1002/jobm.70029","DOIUrl":"https://doi.org/10.1002/jobm.70029","url":null,"abstract":"","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70029"},"PeriodicalIF":3.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular Characterization of Bacterial Cellulose Producing Bacillus Strains Isolated From Soil.
IF 3.5 4区 生物学
Journal of Basic Microbiology Pub Date : 2025-03-28 DOI: 10.1002/jobm.70026
Idris Bektas, Nazli Betul Yildirim
{"title":"Molecular Characterization of Bacterial Cellulose Producing Bacillus Strains Isolated From Soil.","authors":"Idris Bektas, Nazli Betul Yildirim","doi":"10.1002/jobm.70026","DOIUrl":"https://doi.org/10.1002/jobm.70026","url":null,"abstract":"<p><p>Bacterial cellulose (BC) a remarkable natural biopolymer, offering diverse applications across various industries. This study aimed to identify bacteria that are capable of producing bacterial cellulose through the utilization of various carbon sources isolated from soil. BC producing strains were identified as Bacillus amyloliquefaciens B1, Bacillus subtilis B2, Bacillus velezensis B3, Bacillus siamensis B4, and B. amyloliquefaciens B5 by 16 s rDNA sequencing analysis. The highest amount of BC production observed was when Sucrose was used as the carbon source, the trend B. siamensis B4 (3.0 ± 0.35 g L<sup>-1</sup>) > B. subtilis B2 (2.8 ± 0.57 g L<sup>-1</sup>) > B. velezensis B3 (2.5 ± 0.85 g L<sup>-1</sup>) > B. amyloliquefaciens B1 (2.3 ± 0.68 g L<sup>-1</sup>) > B. amyloliquefaciens B5 (2 ± 0.71 g L<sup>-1</sup>). The antibacterial activity of BCs was examined against Escherichia coli and Staphylococcus aureus. The BC produced by B. amyloliquefaciens B5 showed the highest antibacterial activity against S. aureus and and E. coli by producing a clear inhibition zone of 11 and 9 mm, respectively. The water retention capability (WRC %) of the BCs demonstrated a notable range, measuring between 177.7% and 150%. Additionally, the moisture content (MC %) of these samples varied, falling within a range of 64%-60%. The formation of BCs was detected by FTIR analysis. The BC membrane were exhibited definitive characteristics, including a robust nanodimensional network and microfibrils, as evidenced by scanning electron microscopy (SEM). Furthermore, investigation molecular basis of cellulose synthesis of Bacillus strains during cellulose synthesis could offer valuable insights for optimizing production processes.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70026"},"PeriodicalIF":3.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Autoinducer-2 Quorum Sensing Is an Active Universal Signaling System in Sociomicrobiology.
IF 3.5 4区 生物学
Journal of Basic Microbiology Pub Date : 2025-03-28 DOI: 10.1002/jobm.70024
Dingqi Liu, Wei Zhang, Li Zhu, Jinghai Gong, Yi Huang, Zhou Li, Jin He
{"title":"Autoinducer-2 Quorum Sensing Is an Active Universal Signaling System in Sociomicrobiology.","authors":"Dingqi Liu, Wei Zhang, Li Zhu, Jinghai Gong, Yi Huang, Zhou Li, Jin He","doi":"10.1002/jobm.70024","DOIUrl":"https://doi.org/10.1002/jobm.70024","url":null,"abstract":"<p><p>Autoinducer-2 (AI-2) is a bacterial quorum sensing (QS) signaling molecule that regulates inter-specific and intraspecific bacterial communication in complex ecological environments. Here, we systematically analyzed the distribution of AI-2 QS-related proteins (synthases and receptors) in the domain bacteria, explored the evolution and development of AI-2 receptors, and analyzed the AI-2 regulatory networks using human intestinal metagenomic data. The results show that AI-2 QS-related proteins are distributed in 17 bacterial phyla, accounting for approximately 36.80% of the total genomes. Based on the ability of bacteria to synthesize and receive AI-2 signals, we divided bacteria into four major categories, namely Prosumer, Producer, Monitor, and Immunizer. The Gram-positive bacteria are mainly responsible for producing the AI-2 signals, while Gram-negative bacteria are more likely to respond to AI-2. Evolutionary analysis shows that the AI-2 receptor CahR is mainly derived from the methyl-accepting chemotaxis protein (MCP). Based on the length difference of the ligand-binding domains, we further speculate that AI-2 binding activates CahR through either allostery or aggregation mode. Using human intestinal metagenomic data, we found a strong correlation between AI-2 signaling and c-di-GMP signaling. These findings will have an important impact on the AI-2 QS research and accelerate its development.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e024"},"PeriodicalIF":3.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of Biocontrol of Mycosphaerella fijiensis by five Species of Rhizosphere Fungi of Plantain.
IF 3.5 4区 生物学
Journal of Basic Microbiology Pub Date : 2025-03-23 DOI: 10.1002/jobm.70020
Ousmane Fofana, Rosin Don Rodrigue Voko Bi, Kouassi Clément Kouassi
{"title":"Evaluation of Biocontrol of Mycosphaerella fijiensis by five Species of Rhizosphere Fungi of Plantain.","authors":"Ousmane Fofana, Rosin Don Rodrigue Voko Bi, Kouassi Clément Kouassi","doi":"10.1002/jobm.70020","DOIUrl":"https://doi.org/10.1002/jobm.70020","url":null,"abstract":"<p><p>This study assessed the efficacy of certain rhizosphere fungi isolated from banana plantations in the Haut-Sassandra region as biocontrol agents against Mycosphaerella fijiensis, responsible for banana black spot. It involved characterizing banana leaf and rhizosphere fungi and studying the antagonistic activity of rhizosphere fungi on M. fijiensis. Analyses identified isolates M9 and M10 as M. fijiensis. Five fungal species were isolated from the rhizosphere: T. ghanense, T. longibrachiatum, T. reesei, Neurospora dictyophora and H. terricola. In vitro tests revealed that these fungi inhibit the growth of M. fijiensis, using antagonistic mechanisms such as competition, antibiosis and mycoparasitism. The strongest antagonistic activity was observed with N. dictyophora. Moreover, the extracts derived from these antagonists showed high antifungal activity, with total inhibition of M. fijiensis from 2.5 mg/mL upwards. The minimum fungicidal concentration (MFC) for all extracts was 5 mg/mL, except for T. ghanense which showed an MFC of 10 mg/mL. These results suggest that the rhizospheric fungi studied are promising candidates for the development of M. fijiensis biocontrol strategies.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70020"},"PeriodicalIF":3.5,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Biological Characteristics of Nematophagous Fungus Esteya vermicola Fxy121 Strain and Its Specific Detection.
IF 3.5 4区 生物学
Journal of Basic Microbiology Pub Date : 2025-03-23 DOI: 10.1002/jobm.70019
Xiaojian Wen, Yongxia Li, Fangtong Chen, Xuan Wang, Wei Zhang, Lin Huang, Xingyao Zhang
{"title":"The Biological Characteristics of Nematophagous Fungus Esteya vermicola Fxy121 Strain and Its Specific Detection.","authors":"Xiaojian Wen, Yongxia Li, Fangtong Chen, Xuan Wang, Wei Zhang, Lin Huang, Xingyao Zhang","doi":"10.1002/jobm.70019","DOIUrl":"https://doi.org/10.1002/jobm.70019","url":null,"abstract":"<p><p>Esteya vermicola Fxy121 strain, isolated from live pine trees, exhibited high adhesion and infection activity against Bursaphelenchus xylophilus. In this study, we studied the biological characteristics of Esteya vermicola Fxy121, and screened species-specific primers used for its colonization detection on pine trees. The liquid fermentation formula with higher blastospore production was obtained by adjusting the ratio of carbon and nitrogen and adding mineral salts, blastospore concentration was 5.5 × 10<sup>8</sup> spores mL<sup>-1</sup> cultured for 6 days using new formula. The germination of blastospores was not affected when suspension was placed at 4°C-30°C for 1-8 h. The number of lunate spores increased first and then decreased with the increase of initial blastospore concentration of E. vermicola Fxy121, medium concentration of blastospores suspension showed higher infectivity. The evF/evR primer pair successfully amplified the except fragment size of 522 bp from isolates of E. vermicola and from pine needles sprayed with blastospores suspension of E. vermicola. This study suggests that blastospores concentration of E. vermicola Fxy121 significantly affect the production of lunate spores and infectivity against B. xylophilus. The species-specific primers allowed fast and reliable detection of E. vermicola directly from tissues of pine trees sprayed and injected with spore suspension.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70019"},"PeriodicalIF":3.5,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bioprospecting Cold-Adapted Indole Acetic Acid-Producing Bacteria With Multifaceted Plant Growth Promoting Traits for Wheat Growth From Ghulkin Glacier, Pakistan. 对巴基斯坦古尔金冰川中具有促进小麦生长的多方面植物生长性状的冷适应吲哚乙酸生产细菌进行生物勘探。
IF 3.5 4区 生物学
Journal of Basic Microbiology Pub Date : 2025-03-20 DOI: 10.1002/jobm.70017
Muhammad Irfan, Naseer Ahmad, Muhammad Rafiq, Jemma L Wadham, Christopher J Williamson, Abdul Haleem, Noor Hassan, Aamer Ali Shah, Fariha Hasan
{"title":"Bioprospecting Cold-Adapted Indole Acetic Acid-Producing Bacteria With Multifaceted Plant Growth Promoting Traits for Wheat Growth From Ghulkin Glacier, Pakistan.","authors":"Muhammad Irfan, Naseer Ahmad, Muhammad Rafiq, Jemma L Wadham, Christopher J Williamson, Abdul Haleem, Noor Hassan, Aamer Ali Shah, Fariha Hasan","doi":"10.1002/jobm.70017","DOIUrl":"https://doi.org/10.1002/jobm.70017","url":null,"abstract":"<p><p>Agriculture in high altitude regions is challenged by low temperatures, and the use of chemical fertilizers turned hazardous in the long run. In addition, cost-effective, eco-friendly biofertilizers developed globally are less effective in cold regions due to temperature-sensitive mesophilic homologs. The current study evaluated the potential of a cold-adapted indole acetic acid (IAA)-producing bacterial consortium isolated from Ghulkin glacier, Hunza Valley, Pakistan, to promote the growth of the crop, wheat, under cold stress. Several IAA-producing bacteria were isolated and tested further for siderophore production, zinc-solubilizing activity, phosphate solubilizing activity, ability to utilize 1-aminocyclopropane-1-carboxylate and for nitrogen fixation. A microbial consortium was constructed and evaluated for its effectiveness in promoting wheat growth in pot experiments under ambient conditions (2°C-6°C at nighttime and 15°C-20°C at daytime) and with diverse soil and glacial flour matrices. The cold-adapted bacteria showed positive plant growth promoting attributes and with remarkable positive impacts on root length, shoot length, root and shoot fresh, and dry weight in pot trials in both garden soil and the glacial flour. An increase in wheat plant chlorophyll content was also observed in both pot experiments of glacial flour and garden soil. It was concluded that a multistrain bacterial consortium of Nocardiooides zeae GB3, Arthrobacter flavus GA9, and Pseudomonas sp. GB29 may have potential as an efficient biofertilizer for promoting mountainous crops, in the Gilgit Baltistan region, Pakistan.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70017"},"PeriodicalIF":3.5,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Context-Dependent Food Preferences and Comparative Decision-Making in Slime Mold Physarella oblonga.
IF 3.5 4区 生物学
Journal of Basic Microbiology Pub Date : 2025-03-17 DOI: 10.1002/jobm.70023
Hao-Yun Yin, Pin-Jhu Wang, Dong-Sheng Yang, Jui-Yu Chou
{"title":"Context-Dependent Food Preferences and Comparative Decision-Making in Slime Mold Physarella oblonga.","authors":"Hao-Yun Yin, Pin-Jhu Wang, Dong-Sheng Yang, Jui-Yu Chou","doi":"10.1002/jobm.70023","DOIUrl":"https://doi.org/10.1002/jobm.70023","url":null,"abstract":"<p><p>Slime molds, despite their unicellular nature, exhibit complex behaviors and problem-solving abilities that have fascinated researchers. They are widely utilized as model organisms in behavioral studies. In this study, we examined the feeding preferences of the acellular slime mold Physarella oblonga when exposed to different yeast species as food sources. Our findings reveal significant preferences shown by P. oblonga for specific yeast strains. When Wickerhamomyces anomalus JYC2560 was another option, P. oblonga significantly favored Hanseniaspora osmophila JYC2504 (p < 0.05). Similarly, when Kazachstania exigua JYC2539 was another choice, P. oblonga exhibited a significant preference for Dekkera bruxellensis JYC2592 (p < 0.05). However, no significant preference was observed in other pair experiments. Further investigations highlighted variations in responses among different slime mold strains. Additionally, introducing a third yeast species as a decoy showed that P. oblonga preferred Hanseniaspora osmophila JYC2504 over Dekkera bruxellensis JYC2592 (p < 0.001), indicating a decoy effect. The preference can be changed if different yeast strains are used as decoys. Notably, this effect persisted even when the decoy yeast was fed to the slime mold in advance of the choice experiment, suggesting memory retention in slime molds. These findings contribute to our understanding of the intricate interactions between slime molds and their environment, emphasizing the diverse responses observed across different strains.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70023"},"PeriodicalIF":3.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Screening and Identification of Biocontrol Bacteria for Anthracnose in Mahonia eurybracteata Fedde.
IF 3.5 4区 生物学
Journal of Basic Microbiology Pub Date : 2025-03-11 DOI: 10.1002/jobm.70021
Qingyin Sun, Lisha Jiao, Qidan Chen, Huilin Shu, Xiyang Li, Zilong Li
{"title":"Screening and Identification of Biocontrol Bacteria for Anthracnose in Mahonia eurybracteata Fedde.","authors":"Qingyin Sun, Lisha Jiao, Qidan Chen, Huilin Shu, Xiyang Li, Zilong Li","doi":"10.1002/jobm.70021","DOIUrl":"https://doi.org/10.1002/jobm.70021","url":null,"abstract":"<p><p>Mahonia eurybracteata exhibits characteristics related to heat dissipation, nutritional supplementation, and possesses antimicrobial and anticancer properties. Despite its widespread cultivation through artificial means, the detrimental impact of anthracnose on its growth has led to extensive reliance on chemical pesticides for disease control. Unscientific pesticide application and the use of outdated spraying techniques contribute to the presence of pesticide residues in both plants and the environment, thereby posing potential risks. This study utilized tissue isolation to segregate the pathogenic fungus causing anthracnose. The isolated strains, identified through 18S rRNA, were identified as Colletotrichum fructicola and Colletotrichum gloeosporioides. Koch's postulates were employed to confirm the pathogenicity of these strains. Simultaneously, soil samples from the rhizosphere of healthy M. eurybracteata were collected. Using the dilution plate method, 14 bacterial strains were isolated, and molecular biology techniques were employed for preliminary and subsequent screening via the agar confrontation method. Results indicated that selected strains of Pseudomonas gessardii and Pseudomonas fluorescens exhibited inhibitory effects on C. fructicola and C. gloeosporioides, respectively. Through in vitro detached leaf experiments, the biocontrol effectiveness of these two bacterial strains was validated. This study establishes a theoretical foundation for the development and application of antagonistic bacteria.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70021"},"PeriodicalIF":3.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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