{"title":"Actinobacteria From Bryophytes for Growth Promotion of Tropical Moss Species.","authors":"Mathurin Meethangdee, Narin Printarakul, Wasu Pathom-Aree","doi":"10.1002/jobm.70071","DOIUrl":null,"url":null,"abstract":"<p><p>Actinobacteria have shown significant potential in promoting the growth of various plant species, especially those that inhabit in association with plants. However, previous research has mainly focused on higher plant species. Bryophytes are a large group of non-vascular, non-flowering land plants without true vascular bundles, roots, stems, and leaves. Despite the extensive diversity of bryophytes in Thailand, research on the diversity of actinobacteria associated with bryophytes remains scarce. This study aimed to isolate actinobacteria from three bryophyte species in northern Thailand: Koponobryum papillosum Printarakul & Chantanaorr, Pseudotrismegistia undulata, and Sphagnum cuspidatulum C. Müll. A total of 52 isolates were identified, including 6 endophytic and 46 epiphytic isolates. These isolates were assigned to four genera: Brevibacterium (1 isolate), Microbacterium (1 isolate), Rhodococcus (6 isolates), and Streptomyces (44 isolates). All isolates were assessed for their plant-growth promoting abilities both in vitro and in planta. Most actinobacteria exhibited the in vitro ability to produce indole-3-acetic acid (IAA), siderophores, and solubilize tricalcium phosphate. Three isolates-Streptomyces brevispora RHP2, S. halstedii WS1, and Rhodococcus triatomae RHK22-were selected to evaluate their potential to enhance the growth of S. cuspidatulum C. Müll in soil. R. triatomae RHK22 was highly effective in promoting the regeneration of new shoots and significantly enhancing thallus height, fresh weight, dry weight, total chlorophyll content, and total carotenoid content (p < 0.05) of S. cuspidatulum C. Müll when applied as cell suspension and cell free supernatant.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70071"},"PeriodicalIF":3.5000,"publicationDate":"2025-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Basic Microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1002/jobm.70071","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
Actinobacteria have shown significant potential in promoting the growth of various plant species, especially those that inhabit in association with plants. However, previous research has mainly focused on higher plant species. Bryophytes are a large group of non-vascular, non-flowering land plants without true vascular bundles, roots, stems, and leaves. Despite the extensive diversity of bryophytes in Thailand, research on the diversity of actinobacteria associated with bryophytes remains scarce. This study aimed to isolate actinobacteria from three bryophyte species in northern Thailand: Koponobryum papillosum Printarakul & Chantanaorr, Pseudotrismegistia undulata, and Sphagnum cuspidatulum C. Müll. A total of 52 isolates were identified, including 6 endophytic and 46 epiphytic isolates. These isolates were assigned to four genera: Brevibacterium (1 isolate), Microbacterium (1 isolate), Rhodococcus (6 isolates), and Streptomyces (44 isolates). All isolates were assessed for their plant-growth promoting abilities both in vitro and in planta. Most actinobacteria exhibited the in vitro ability to produce indole-3-acetic acid (IAA), siderophores, and solubilize tricalcium phosphate. Three isolates-Streptomyces brevispora RHP2, S. halstedii WS1, and Rhodococcus triatomae RHK22-were selected to evaluate their potential to enhance the growth of S. cuspidatulum C. Müll in soil. R. triatomae RHK22 was highly effective in promoting the regeneration of new shoots and significantly enhancing thallus height, fresh weight, dry weight, total chlorophyll content, and total carotenoid content (p < 0.05) of S. cuspidatulum C. Müll when applied as cell suspension and cell free supernatant.
放线菌在促进各种植物的生长方面显示出巨大的潜力,特别是那些与植物共生的植物。然而,以往的研究主要集中在高等植物物种上。苔藓植物是一大群没有维管束、根、茎和叶的无维管束、不开花的陆地植物。尽管泰国苔藓植物多样性广泛,但对与苔藓植物相关的放线菌多样性的研究仍然很少。本研究旨在从泰国北部3种苔藓植物中分离放线菌:Koponobryum papillosum Printarakul & chantanorr、Pseudotrismegistia undulata和Sphagnum cuspidatulum C. m ll。共分离到52株,其中内生分离株6株,附生分离株46株。这些分离株被划分为4个属:短杆菌(1株)、微杆菌(1株)、红球菌(6株)和链霉菌(44株)。对所有分离株进行了体外和体内促生长能力评价。大多数放线菌在体外表现出产生吲哚-3-乙酸(IAA)、铁载体和溶解磷酸三钙的能力。选取短孢链霉菌RHP2、halstedii链霉菌WS1和三角红球菌rhk22三种分离菌株,研究其对cuspidatulum C. m ll在土壤中的生长促进作用。RHK22在促进新梢再生和显著提高组织高、鲜重、干重、总叶绿素含量和总类胡萝卜素含量方面具有显著的促进作用
期刊介绍:
The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions.
Papers published deal with:
microbial interactions (pathogenic, mutualistic, environmental),
ecology,
physiology,
genetics and cell biology/development,
new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications)
novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).