Journal of Basic Microbiology最新文献_第3页

Optimized Production and Characterization of Auxin by Bacillus amyloliquefaciens (TSP11) for Plant Growth Promotion.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-06 DOI: 10.1002/jobm.70010

Thangarasu Suganya Devi, Karuppiah Vijay, Raja Mythili, Mohammad Ahmad Wadaan, Vasubalan Manivinoth, Woong Kim, Thangavel Kavitha

{"title":"Optimized Production and Characterization of Auxin by Bacillus amyloliquefaciens (TSP11) for Plant Growth Promotion.","authors":"Thangarasu Suganya Devi, Karuppiah Vijay, Raja Mythili, Mohammad Ahmad Wadaan, Vasubalan Manivinoth, Woong Kim, Thangavel Kavitha","doi":"10.1002/jobm.70010","DOIUrl":"https://doi.org/10.1002/jobm.70010","url":null,"abstract":"This study was aimed at isolating and recognizing the plant growth promoting rhizobacteria that secretes indole-3-acetic acid/auxin hormones. Among the 82 pure cultured bacterial morphotypes, the strain TSP11 showed high auxin production utilizing rice husk as a cheap substitute in the production medium. The minimal medium supplemented with 0.1 g/mL L-tryptophan and 1.29% rice husk produced the highest amount of auxin. Synthesized auxin was purified and quantified by chromatographic techniques. 120.08 µg/mL of synthetic medium and 135 µg/mL of agro-waste medium were the observed yields in HPLC quantification. Further GC-MS/MS and LC-MS/MS spectra exposed an existence of derived compounds such as 1H-indole, 7-methyl, indole, 3-methyl-, 1H-indole-3-carboxaldehyde (1H-indol-3-YL)-acetic acid. It was characterized by X-ray diffraction to reveal its crystallinity. Thermo gravimetric analysis showed the thermal stability of auxin over the range between 200°C and 300°C. Structural predictions of extracted auxin through HR-NMR revealed chemical shifts for Indole and acetate derivatives. The potent producer was identified as Bacillus amyloliquefaciens strain TSP11based on 16SrDNA gene sequencing. Increased seed germination percentage was observed when compared to untreated control. The research findings explore the possibility of exploiting B. amyloliquefaciens strain TSP11for bioformulations. This study represents an initial investigation into the plant growth-promoting potential of B. amyloliquefaciens strain TSP11, particularly regarding its auxin production using rice husk as a medium. We will explore the exact mechanism of auxin synthesis and its impact on various plant species in further studies.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70010"},"PeriodicalIF":3.5,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Issue Information: Journal of Basic Microbiology. 3/2025

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-05 DOI: 10.1002/jobm.202570006

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Cover: Journal of Basic Microbiology. 3/2025

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-05 DOI: 10.1002/jobm.202570005

引用次数: 0

Harnessing Microbial Consortia for Sustainable Management of Corm Rot of Saffron.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-02-25 DOI: 10.1002/jobm.70009

Ayushi Sharma, Vishal Gupta, Gayatri Jamwal, Sushil K Gupta, Vinod Pandit, Zakir Amin, Fayaz A Mohiddin, Khalid E Hamed, Suhail Ashraf

{"title":"Harnessing Microbial Consortia for Sustainable Management of Corm Rot of Saffron.","authors":"Ayushi Sharma, Vishal Gupta, Gayatri Jamwal, Sushil K Gupta, Vinod Pandit, Zakir Amin, Fayaz A Mohiddin, Khalid E Hamed, Suhail Ashraf","doi":"10.1002/jobm.70009","DOIUrl":"https://doi.org/10.1002/jobm.70009","url":null,"abstract":"Saffron (Crocus Sativus L.) is a highly prized spice crop renowned for its significant culinary and therapeutic applications, largely attributed to its bioactive constituents such as crocin, picrocrocin, and safranal. Despite its production is severely threatened by corm rot, particularly caused by the pathogenic fungus Fusarium oxysporum, which leads to substantial yield losses in major saffron-producing regions. This review highlights the promising role of microbial consortia as an eco-friendly and sustainable strategy for effectively managing corm rot disease. The presence of diverse microbial communities can improve defense mechanisms of a plant against pathogens and support its overall growth and productivity. The mechanisms by which these microbial interactions occur, including nutrient competition, biofilm formation, and the activation of systemic resistance, contribute to their combined efficacy in inhibiting fungal pathogens. The necessity for innovative microbial strategies that harness the combined benefits of diverse microorganisms to mitigate corm rot and improve saffron cultivation sustainably. As sustainable agricultural practices increasingly gain prominence, the development and application of tailored microbial consortia gave a promising alternative to chemical fungicides, ensuring both economic viability and ecological balance in saffron production.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70009"},"PeriodicalIF":3.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Harnessing Fungal Bioagents Rich in Volatile Metabolites for Sustainable Crop Protection: A Critical Review

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-02-25 DOI: 10.1002/jobm.70003

Dheeraj Kumar, Somnath Roy, Azariah Babu, Abhay K. Pandey

{"title":"Harnessing Fungal Bioagents Rich in Volatile Metabolites for Sustainable Crop Protection: A Critical Review","authors":"Dheeraj Kumar, Somnath Roy, Azariah Babu, Abhay K. Pandey","doi":"10.1002/jobm.70003","DOIUrl":"10.1002/jobm.70003","url":null,"abstract":"<div>\u0000 \u0000 Pests and diseases have a significant impact on crop health and yields, posing a serious threat to global agriculture. Effective management strategies, such as integrated pest management (IPM), including crop rotation, use of synthetic pesticides, biological control, and resistant/tolerant crop varieties, are essential to mitigate these risks and ensure sustainable agricultural practices. Fungal bioagents play an important role in managing phytopathogens and insect pests by acting as biological agents. They promote healthy plant growth by enhancing the uptake of nutrients and combating systemic resistance in plants. Furthermore, fungal bioagents are environmentally friendly, reducing application of fungicides and insecticides and minimizing their negative impact on the crops and environment. Their use in IPM promotes sustainable agriculture and ensures high-quality crops while maintaining soil health and microbial biodiversity. These fungal bioagents are rich sources of volatile organic compounds (VOCs), which play an important role in biological communication during interaction with insect pests and phytopathogens. In pest management, VOC production by beneficial fungi is accountable for their efficacy against pests and pathogens. Thus, this review discusses the important fungal bioagents producing VOCs, extraction methods of VOC, and the use of VOC-producing fungi in pest and disease management, knowledge gaps, and future research areas.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143501112","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolomic Profiling and Genome-Wide Analysis of Bacillus subtilis NBAIR-BSWG1 Reveals Cyclic Lipopeptides as Key Antagonists.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-02-23 DOI: 10.1002/jobm.70007

Ankitha Karegowdru Surendra, Radha Tumkur Kumaraswamy, Ruqiya Seegenahalli, Aditya Kukreti, Lavanya Siddanahalli Manjunatha, Kandan Aravindaram, Sivakumar Gopalsamy, Ramya Ramesan Syamala, Manjunatha Channappa

{"title":"Metabolomic Profiling and Genome-Wide Analysis of Bacillus subtilis NBAIR-BSWG1 Reveals Cyclic Lipopeptides as Key Antagonists.","authors":"Ankitha Karegowdru Surendra, Radha Tumkur Kumaraswamy, Ruqiya Seegenahalli, Aditya Kukreti, Lavanya Siddanahalli Manjunatha, Kandan Aravindaram, Sivakumar Gopalsamy, Ramya Ramesan Syamala, Manjunatha Channappa","doi":"10.1002/jobm.70007","DOIUrl":"https://doi.org/10.1002/jobm.70007","url":null,"abstract":"Understanding bacterial genetics and metabolism is vital for developing biopesticides. This study investigates Bacillus subtilis NBAIR-BSWG1, a strain well known for its antagonistic potential. Crude lipopeptides extracted from the strain were evaluated for in vitro activity, showing complete inhibition of Rhizoctonia solani at a concentration of 50 μL/mL potato dextrose agar. To delve deeper into its antagonistic mechanisms, we conducted whole-genome sequencing of NBAIR-BSWG1 using Illumina NextSeq 500. Subsequent analysis with the BlastX diamond tool revealed 19 key biosurfactant genes, including surfactin (srfAA, srfAC, srfAD, srfP), fengycin (ppsE, ppsD, ppsC, ppsB), and putisolvin (dnaK), which were further confirmed by PCR using specific primers. Meanwhile, antiSMASH analysis revealed gene clusters with 100% similarity to those responsible for the synthesis of fengycin, bacilaene, bacillibactin, subtilosin A, and bacilysin, as well as clusters with 82% similarity to surfactin synthesis genes. Additionally, liquid chromatography-mass spectrometry was performed to analyze the cell-free extract produced by NBAIR-BSWG1, revealing the presence of various cyclic lipopeptides, including multiple peaks corresponding to surfactin, iturin, and several novel lipopeptide compounds. This study highlights B. subtilis NBAIR-BSWG1 cyclic lipopeptides as a key to broad-spectrum bio-control and establishes the strain as highly potent.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70007"},"PeriodicalIF":3.5,"publicationDate":"2025-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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The Interactive Dynamics of Pseudomonas aeruginosa in Global Ecology

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-02-19 DOI: 10.1002/jobm.70004

Telma de Sousa, Catarina Silva, Gilberto Igrejas, Michel Hébraud, Patrícia Poeta

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Comparative Analysis of phaC1 and phaC2 Genes Encoding Polyhydroxyalkanoate Synthase in Cupriavidus malaysiensis USMAA1020^T.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-02-18 DOI: 10.1002/jobm.70008

Nur Asilla Hani Shafie, Nor Azura Azami, Nyok Sean Lau, Siti Nor Syairah Anis, Amirul Al-Ashraf Abdullah

{"title":"Comparative Analysis of phaC1 and phaC2 Genes Encoding Polyhydroxyalkanoate Synthase in Cupriavidus malaysiensis USMAA1020T.","authors":"Nur Asilla Hani Shafie, Nor Azura Azami, Nyok Sean Lau, Siti Nor Syairah Anis, Amirul Al-Ashraf Abdullah","doi":"10.1002/jobm.70008","DOIUrl":"https://doi.org/10.1002/jobm.70008","url":null,"abstract":"Two polyhydroxyalkanoate synthase genes, phaC1 and phaC2, were identified in three strains of Cupriavidus malaysiensis (C. malaysiensis): C. malaysiensis USMAA1020T, C. malaysiensis USMAHM13, and C. malaysiensis USMAA2-4. Interestingly, the genome of C. malaysiensis USMAA1020T revealed the presence of the polyhydroxyalkanoate granule-associated protein (phaF), which was not present in C. malaysiensis USMAHM13 and C. malaysiensis USMAA2-4. A Maximum Likelihood phylogenetic analysis shows that the phaC genes were classified into Class I synthases. The phaC1 and phaC2 genes in the three C. malaysiensis strains formed a separate, distinct cluster. To further examine the function of phaC, both phaC genes were cloned from C. malaysiensis USMAA1020T and individually expressed in Cupriavidus necator (C. necator) PHB-4, which serves as a benchmark of functionality for other strains. Using γ-butyrolactone as the sole carbon source, the poly(3-hydroxybutyrate-co-4-hydroxybutyrate) contains up to 83.00 mol% 4-hydroxybutyrate (4HB) and 26.50% PHA content. However, the transformant C. necator PHB-4 with phaC2 produced only 2.30% PHA content and no 4HB monomer. The phaC2 transformant produces up to 100 mol% 3HB monomer and 41.90% PHA content, while the phaC1 transformant produces only 25.80% PHA content when using oleic acid as the sole carbon source. When provided with a mixed substrate of oleic acid and 1-pentanol, the transconjugants accumulated up to 20% PHA content but produced a low 3HV content of only 4%-5%. These findings significantly contribute to the scientific literature by improving the understanding of the genetic and biochemical diversity of the two PHA synthases, phaC1 and phaC2, in Cupriavidus species.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70008"},"PeriodicalIF":3.5,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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DGK1 as a Target of Gemfibrozil to Induce Lipid Accumulation via the Transcription Factors TUP1/CYC8 in Saccharomyces cerevisiae.

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-02-18 DOI: 10.1002/jobm.70005

Xiaojuan Zhang, Yao Liu, Weiwei Liu, Yuhu Shen, Fangqi Cao, Yunxia Deng, Zhangjun Cao, Zhiwei Huang

{"title":"DGK1 as a Target of Gemfibrozil to Induce Lipid Accumulation via the Transcription Factors TUP1/CYC8 in Saccharomyces cerevisiae.","authors":"Xiaojuan Zhang, Yao Liu, Weiwei Liu, Yuhu Shen, Fangqi Cao, Yunxia Deng, Zhangjun Cao, Zhiwei Huang","doi":"10.1002/jobm.70005","DOIUrl":"https://doi.org/10.1002/jobm.70005","url":null,"abstract":"Gemfibrozil (GEM) is a phenoxy aromatic acid-based lipid-lowering drug. It activates peroxisome proliferator-activated receptor alpha (PPAR-α), which leads to altered lipid metabolism and lowers serum triglyceride levels by modulating lipoprotein lipase. However, the action of the mode of GEM is still unclear. Herein, the model organism Saccharomyces cerevisiae was applied to explore the molecular mechanism of GEM regulating lipid metabolism. The results showed that the triacylglycerol (TAG) content and the number of lipid droplets of yeast increased significantly after GEM treatment in the wild-type BY4741. Screening of mutations related to lipid metabolism pathways (PAH1, DGK1, TGL3, TGL4, LRO1, ARE1, ARE2, and DGA1) showed that dgk1Δ had no change in lipid accumulation under GEM. In the wild type, GEM inhibited the expression of DGK1, resulting in a significant decrease in the contents of phospholipids (phosphatidylcholine (PC), phosphatidylethanolamine (PE), and phosphatidylserine (PS)) and neutral lipids (TAG and diacylglycerol (DAG)). However, their abundances could not be changed in dgk1Δ after the treatment with GEM Luciferase assay further showed that DGK1 may be a target of GEM to induce lipid accumulation via TUP1/CYC8, which could act on the DGK1 promoter-TATA highly conserved element (-400 bp - 200 bp). Altogether, the effect of GEM on lipid metabolism was associated with the upregulation of TUP1/CYC8, leading to a decrease in the expression of DGK1, thereby increasing the TAG content in yeast cells. It is expected that the data will help to clarify the molecular mechanism of GEM regulating lipid metabolism in humans.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70005"},"PeriodicalIF":3.5,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143440787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Issue Information: Journal of Basic Microbiology. 2/2025

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-02-12 DOI: 10.1002/jobm.202570004

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