{"title":"Genetic Adaptations and Mechanistic Insights Into Bacterial Bioremediation in Ecosystems","authors":"Yamini Vinayagam, Vijayarangan Devi Rajeswari","doi":"10.1002/jobm.202400387","DOIUrl":"10.1002/jobm.202400387","url":null,"abstract":"<div>\u0000 \u0000 <p>Metal pollution poses significant threats to the ecosystem and human health, demanding effective remediation strategies. Bioremediation, which leverages the unique metal-resistant genes found in bacteria, offers a cost-effective and efficient solution to heavy metal contamination. Genes such as Cad, Chr, Cop, and others provide pathways to improve the detoxification of the ecosystem. Through multiple techniques, genetic engineering makes bacterial genomes more capable of improving metal detoxification; nonetheless, there are still unanswered questions regarding the nature of new metal-resistant genes. This article examines bacteria's complex processes to detoxify toxic metals, including biosorption, bioaccumulation, bio-precipitation, and bioleaching. It also explores essential genes, proteins, signaling mechanisms, and bacterial biomarkers involved in breaking toxic metals.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142154075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Athira G. Menon, Haseena Bhaskar, K. Surendra Gopal, Rajkumar M, Smitha M. Subramanian
{"title":"Symbiotic and Nonsymbiotic Bacteria Associated With the Entomo-Pathogenic Nematode, Heterorhabditis spp (Rhabditida: Heterorhabditidae) From South India","authors":"Athira G. Menon, Haseena Bhaskar, K. Surendra Gopal, Rajkumar M, Smitha M. Subramanian","doi":"10.1002/jobm.202400108","DOIUrl":"10.1002/jobm.202400108","url":null,"abstract":"<div>\u0000 \u0000 <p>Sixteen isolates of bacteria obtained from the entomopathogenic nematode (<i>Heterorhabditis</i> sp.) infected cadavers of <i>Galleria mellonella</i> larvae were identified following phenotypic characterization and molecular analysis of <i>16S rRNA</i>. Two isolates were identified as the symbiotic bacterium, <i>Photothabdus luminescens</i>, while 14 other isolates were represented by nine species of nonsymbiotic bacteria viz., <i>Stenotrophomonas maltophilia</i>, <i>Alcaligenes aquatilis, Brevundimonas diminuta</i>, <i>Brucella pseudointermedia</i>, <i>Ochrobactrum</i> sp., <i>Brucella pseudogrignonensis</i>, <i>Brucella anthropic</i>, <i>Pseudomonas azatoformans</i> and <i>Pseudomonas lactis</i>. The phylogenetic analysis confirmed the evolutionary relationship between <i>P. luminescens</i> and <i>Pseudomonas</i> spp. The study also found a close relationship among the nonsymbiotic bacteria such as <i>A. aquatilis</i>, <i>B. diminuta, Ochrobactrum</i> sp., and <i>Brucell</i>a spp. <i>P. luminescens</i> has been documented for its insecticidal effects against a wide range of insects. The two local isolates obtained in this study may be explored for their biocontrol potential against major pests of the region. Further, the association of nonsymbiotic bacteria with the EPN may be investigated.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142140171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cover: Journal of Basic Microbiology. 9/2024","authors":"","doi":"10.1002/jobm.202470081","DOIUrl":"https://doi.org/10.1002/jobm.202470081","url":null,"abstract":"<p><b>Cover illustration:</b></p><p>Surface morphology of the lichen <i>Dirinaria aegilita</i>. The lichen has a smooth, crustose thallus with a distinct white or gray color. It spreads across the rock surface in a circular pattern.</p><p>(Photo: Shyam Kumar Rajaram, Department of Biotechnology, Kamaraj College of Engineering and Technology, Tamil Nadu, India)\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-09-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.202470081","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142123208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Optimization of Fermentation Conditions of Cordyceps militaris and In Silico Analysis of Antifungal Property of Cordycepin Against Plant Pathogens","authors":"Mansoor Showkat, Nagesha Narayanappa, Nagaraju Umashankar, Benherlal Palayyan Saraswathy, Shobha Doddanagappa, Suhail Ashraf, Saima Gani, Nusrat Fatimah, Asha Nabi, Kahkashan Perveen, Najat A. Bukhari, Jayanthi Barasarathi, Riyaz Z. Sayyed","doi":"10.1002/jobm.202400409","DOIUrl":"10.1002/jobm.202400409","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Cordyceps militaris</i>, a medicinal fungus, has gained considerable attention owing to its potential health benefits, notably the production of bioactive compounds such as cordycepin. Cordycepin possesses significant antifungal, antibacterial, and antiviral properties. The present study focused on optimizing the fermentation conditions for <i>C. militaris</i> to boost the production of mycelia and cordycepin, alongside investigating its antifungal properties using in silico and in vitro approaches. The optimal conditions, yielding the highest cordycepin and mycelial biomass, were a temperature of 20°C and a pH range of 4–6, with glucose and sucrose as carbon sources and yeast extract and casein hydrolysate as nitrogen sources. Under these conditions, cordycepin production peaked at low pH (600–1000 mg/L) and with carbon and maltose (400–500 mg/L). The low temperature favored cordycepin production (400 mg/L), whereas casein hydrolysate as a nitrogen source boosted cordycepin yield (600 mg/L). The docking analysis indicated that cordycepin had the highest binding affinity for the tubulin beta chain 2 (−10.4 kcal/mol) compared to the fungicide tebuconazole (−7.9 kcal/mol for both targets). The in silico results were corroborated by in vitro studies, where the mycelial extract of <i>C. militaris</i> inhibited approximately 75% of fungal growth at a concentration of 6000 ppm. These findings suggest that optimizing fermentation conditions significantly enhances cordycepin production, and cordycepin shows antifungal solid activity, making it a promising agent for biocontrol in agriculture.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"New Aspects of the Effects of Climate Change on Interactions Between Plants and Microbiomes: A Review","authors":"Nilanjan Chakraborty, Sunanda Halder, Chetan Keswani, Jessica Vaca, Aurelio Ortiz, Estibaliz Sansinenea","doi":"10.1002/jobm.202400345","DOIUrl":"10.1002/jobm.202400345","url":null,"abstract":"<div>\u0000 \u0000 <p>One of the most talked about issues of the 21st century is climate change, as it affects not just our health but also forestry, agriculture, biodiversity, the ecosystem, and the energy supply. Greenhouse gases are the primary cause of climate change, having dramatic effects on the environment. Climate change has an impact on the function and composition of the terrestrial microbial community both directly and indirectly. Changes in the prevailing climatic conditions brought about by climate change will lead to modifications in plant physiology, root exudation, signal alteration, and the quantity, makeup, and diversity of soil microbial communities. Microbiological activity is very crucial in organic production systems due to the organic origin of microorganisms. Microbes that benefit crop plants are known as plant growth-promoting microorganisms. Thus, the effects of climate change on the environment also have an impact on the abilities of beneficial bacteria to support plant growth, health, and root colonization. In this review, we have covered the effects of temperature, precipitation, drought, and CO<sub>2</sub> on plant–microbe interactions, as well as some physiological implications of these changes. Additionally, this paper highlights the ways in which bacteria in plants' rhizosphere react to the dominant climatic conditions in the soil environment. The goal of this study is to analyze the effects of climate change on plant–microbe interactions.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142107748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Humaira, Irfan Ahmad, Hafiz Abdullah Shakir, Muhammad Khan, Marcelo Franco, Muhammad Irfan
{"title":"Bacterial Extracellular Vesicles: Potential Therapeutic Applications, Challenges, and Future Prospects","authors":"Humaira, Irfan Ahmad, Hafiz Abdullah Shakir, Muhammad Khan, Marcelo Franco, Muhammad Irfan","doi":"10.1002/jobm.202400221","DOIUrl":"10.1002/jobm.202400221","url":null,"abstract":"<div>\u0000 \u0000 <p>Almost all cell types naturally secret extracellular vesicles (EVs) in the extracellular space with variable metabolic cargo facilitating intracellular communication, posing immune-modulation capacity. Thus, “bacterial extracellular vesicles” (BEVs), with their great immunoregulatory, immune response stimulation and disease condition-altering potential, have gained importance in the medical and therapeutic industry. Various subtypes of BEVs were observed and reported in the literature, such as exosomes (30–150 nm), microvesicles (100–1000 nm), apoptotic bodies (1000–5000 nm), and oncosomes (1000–10,000 nm). As biological systems are complex entities, inserting BEVs requires extra high purity. Various techniques for BEV isolation have been employed alone or with other strategies, such as ultracentrifugation, precipitation, size-exclusion chromatography, affinity-based separation, ultrafiltration, and field-flow fractionation. But to date, no BEV isolation method is considered perfect as the lack of standard protocols limits their scale-up. Medical research has focused on BEVs to explore their diverse therapeutic potential. This review particularly focused on the recent advancements in the potential medical application of BEVs, current challenges, and prospects associated with their scale-up.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141988035","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Cyanobacterial Silver Nanoparticles and Their Potential Utility—Recent Progress and Prospects: A Review","authors":"Maheswari Behera, Prateek Ranjan Behera, Gangadhar Sethi, Biswajita Pradhan, Varanasi Adarsh, Omar Abdurahman Alkilayh, Devi Prasad Samantaray, Lakshmi Singh","doi":"10.1002/jobm.202400256","DOIUrl":"10.1002/jobm.202400256","url":null,"abstract":"<div>\u0000 \u0000 <p>The current situation involves an increase in interest in nanotechnology, in particular the ways in which it can be applied in the commercial and medical fields. However, traditional methods of synthesizing nanoparticles have some drawbacks, including the generation of harmful byproducts, high energy consumption, and cost. As a result, researchers have shifted their focus to “green” nanoparticle synthesis to circumvent these drawbacks. Because of their exceptional physiochemical properties, silver nanoparticles (Ag Nps) are the noble metal nanoparticles that are used most frequently. The green approach to Ag NP synthesis is environmentally friendly, non-toxic, and cost-effective, and it makes use of a variety of biological entities. Cyanobacteria, in particular, have garnered the most attention because of the abundance of bioactive substances that they contain, which serve both as reducing agents and as stabilizing agents during the process of biosynthesis. This review article discusses the current state of cyanobacteria-mediated Ag NP synthesis, the potential mechanisms that are involved, nanoparticle characterization, the various applications of Ag NP in different fields, and their prospects.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901857","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effect of Integrated Nutrient Management on Soil Health, Soil Quality, and Production of Cowpea (Vigna unguiculata L.)","authors":"Gurpreet Kaur, Jupinder Kaur, Sohan Singh Walia","doi":"10.1002/jobm.202400225","DOIUrl":"10.1002/jobm.202400225","url":null,"abstract":"<div>\u0000 \u0000 <p>The integrated application of inorganic fertilizers, organic fertilizers, and biofertilizers helps sustain the nutrient pool and benefits the soil quality, thereby boosting plant health. The effect of different combinations of biofertilizers (consortium biofertilizer [CBF]—non-rhizobial PGPR), inorganic fertilizers, and organic fertilizers on soil health, growth, and yield of cowpea was evaluated by conducting a field experiment. The application of N<sub>100 </sub>FYM + CBF resulted in significantly higher populations of bacteria, fungi, PSB, and diazotroph, as well as soil dehydrogenase and alkaline phosphatase enzyme activities. However, the application of N<sub>100</sub> FYM recorded a significantly higher actinomycetes population. The application of N<sub>100</sub> FYM + CBF resulted in significantly higher soil OC, available nitrogen, phosphorus, and potassium. The soil pH was recorded to be highest in control, and soil EC was recorded to be lowest in control. The plant uptake of nitrogen, phosphorus, and potassium was significantly higher with N<sub>50</sub> FYM + NP<sub>50</sub> + CBF. The root–shoot biomass, number of leaves, nodules/plant, number of pods/plants, pod biomass, pod length, and pod width were significantly higher in treatment having N<sub>50</sub> FYM + NP<sub>50</sub> + CBF. However, the height of the plant, number of branches, and biomass of leaves were highest in treatment with N<sub>25</sub> FYM + NP<sub>75</sub> + CBF. The pod and stover yield were significantly higher in treatment with N<sub>50</sub> FYM + NP<sub>50</sub> + CBF. The results showed that the integrated application of non-rhizobial PGPR along with organic and inorganic fertilizer helps to improve overall soil health, quality, and plant growth of forage cowpea contributing to an increase in crop yield.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141901858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}