Journal of Basic Microbiology最新文献_第4页

Molecular Characterization of Bacterial Cellulose Producing Bacillus Strains Isolated From Soil. 土壤中产纤维素芽孢杆菌的分子特性研究。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-28 DOI: 10.1002/jobm.70026

Idris Bektas, Nazli Betul Yildirim

{"title":"Molecular Characterization of Bacterial Cellulose Producing Bacillus Strains Isolated From Soil.","authors":"Idris Bektas, Nazli Betul Yildirim","doi":"10.1002/jobm.70026","DOIUrl":"https://doi.org/10.1002/jobm.70026","url":null,"abstract":"Bacterial cellulose (BC) a remarkable natural biopolymer, offering diverse applications across various industries. This study aimed to identify bacteria that are capable of producing bacterial cellulose through the utilization of various carbon sources isolated from soil. BC producing strains were identified as Bacillus amyloliquefaciens B1, Bacillus subtilis B2, Bacillus velezensis B3, Bacillus siamensis B4, and B. amyloliquefaciens B5 by 16 s rDNA sequencing analysis. The highest amount of BC production observed was when Sucrose was used as the carbon source, the trend B. siamensis B4 (3.0 ± 0.35 g L-1) > B. subtilis B2 (2.8 ± 0.57 g L-1) > B. velezensis B3 (2.5 ± 0.85 g L-1) > B. amyloliquefaciens B1 (2.3 ± 0.68 g L-1) > B. amyloliquefaciens B5 (2 ± 0.71 g L-1). The antibacterial activity of BCs was examined against Escherichia coli and Staphylococcus aureus. The BC produced by B. amyloliquefaciens B5 showed the highest antibacterial activity against S. aureus and and E. coli by producing a clear inhibition zone of 11 and 9 mm, respectively. The water retention capability (WRC %) of the BCs demonstrated a notable range, measuring between 177.7% and 150%. Additionally, the moisture content (MC %) of these samples varied, falling within a range of 64%-60%. The formation of BCs was detected by FTIR analysis. The BC membrane were exhibited definitive characteristics, including a robust nanodimensional network and microfibrils, as evidenced by scanning electron microscopy (SEM). Furthermore, investigation molecular basis of cellulose synthesis of Bacillus strains during cellulose synthesis could offer valuable insights for optimizing production processes.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70026"},"PeriodicalIF":3.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Autoinducer-2 Quorum Sensing Is an Active Universal Signaling System in Sociomicrobiology. 自诱导-2群体感应是社会微生物学中一种主动的通用信号系统。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-28 DOI: 10.1002/jobm.70024

Dingqi Liu, Wei Zhang, Li Zhu, Jinghai Gong, Yi Huang, Zhou Li, Jin He

{"title":"Autoinducer-2 Quorum Sensing Is an Active Universal Signaling System in Sociomicrobiology.","authors":"Dingqi Liu, Wei Zhang, Li Zhu, Jinghai Gong, Yi Huang, Zhou Li, Jin He","doi":"10.1002/jobm.70024","DOIUrl":"https://doi.org/10.1002/jobm.70024","url":null,"abstract":"Autoinducer-2 (AI-2) is a bacterial quorum sensing (QS) signaling molecule that regulates inter-specific and intraspecific bacterial communication in complex ecological environments. Here, we systematically analyzed the distribution of AI-2 QS-related proteins (synthases and receptors) in the domain bacteria, explored the evolution and development of AI-2 receptors, and analyzed the AI-2 regulatory networks using human intestinal metagenomic data. The results show that AI-2 QS-related proteins are distributed in 17 bacterial phyla, accounting for approximately 36.80% of the total genomes. Based on the ability of bacteria to synthesize and receive AI-2 signals, we divided bacteria into four major categories, namely Prosumer, Producer, Monitor, and Immunizer. The Gram-positive bacteria are mainly responsible for producing the AI-2 signals, while Gram-negative bacteria are more likely to respond to AI-2. Evolutionary analysis shows that the AI-2 receptor CahR is mainly derived from the methyl-accepting chemotaxis protein (MCP). Based on the length difference of the ligand-binding domains, we further speculate that AI-2 binding activates CahR through either allostery or aggregation mode. Using human intestinal metagenomic data, we found a strong correlation between AI-2 signaling and c-di-GMP signaling. These findings will have an important impact on the AI-2 QS research and accelerate its development.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e024"},"PeriodicalIF":3.5,"publicationDate":"2025-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143730250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Biocontrol of Mycosphaerella fijiensis by five Species of Rhizosphere Fungi of Plantain. 五种车前草根际真菌对斐济分枝杆菌的生物防治效果评价。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-23 DOI: 10.1002/jobm.70020

Ousmane Fofana, Rosin Don Rodrigue Voko Bi, Kouassi Clément Kouassi

{"title":"Evaluation of Biocontrol of Mycosphaerella fijiensis by five Species of Rhizosphere Fungi of Plantain.","authors":"Ousmane Fofana, Rosin Don Rodrigue Voko Bi, Kouassi Clément Kouassi","doi":"10.1002/jobm.70020","DOIUrl":"https://doi.org/10.1002/jobm.70020","url":null,"abstract":"This study assessed the efficacy of certain rhizosphere fungi isolated from banana plantations in the Haut-Sassandra region as biocontrol agents against Mycosphaerella fijiensis, responsible for banana black spot. It involved characterizing banana leaf and rhizosphere fungi and studying the antagonistic activity of rhizosphere fungi on M. fijiensis. Analyses identified isolates M9 and M10 as M. fijiensis. Five fungal species were isolated from the rhizosphere: T. ghanense, T. longibrachiatum, T. reesei, Neurospora dictyophora and H. terricola. In vitro tests revealed that these fungi inhibit the growth of M. fijiensis, using antagonistic mechanisms such as competition, antibiosis and mycoparasitism. The strongest antagonistic activity was observed with N. dictyophora. Moreover, the extracts derived from these antagonists showed high antifungal activity, with total inhibition of M. fijiensis from 2.5 mg/mL upwards. The minimum fungicidal concentration (MFC) for all extracts was 5 mg/mL, except for T. ghanense which showed an MFC of 10 mg/mL. These results suggest that the rhizospheric fungi studied are promising candidates for the development of M. fijiensis biocontrol strategies.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70020"},"PeriodicalIF":3.5,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Biological Characteristics of Nematophagous Fungus Esteya vermicola Fxy121 Strain and Its Specific Detection. 噬线虫真菌线虫芽孢杆菌Fxy121的生物学特性及其特异性检测。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-23 DOI: 10.1002/jobm.70019

Xiaojian Wen, Yongxia Li, Fangtong Chen, Xuan Wang, Wei Zhang, Lin Huang, Xingyao Zhang

{"title":"The Biological Characteristics of Nematophagous Fungus Esteya vermicola Fxy121 Strain and Its Specific Detection.","authors":"Xiaojian Wen, Yongxia Li, Fangtong Chen, Xuan Wang, Wei Zhang, Lin Huang, Xingyao Zhang","doi":"10.1002/jobm.70019","DOIUrl":"https://doi.org/10.1002/jobm.70019","url":null,"abstract":"Esteya vermicola Fxy121 strain, isolated from live pine trees, exhibited high adhesion and infection activity against Bursaphelenchus xylophilus. In this study, we studied the biological characteristics of Esteya vermicola Fxy121, and screened species-specific primers used for its colonization detection on pine trees. The liquid fermentation formula with higher blastospore production was obtained by adjusting the ratio of carbon and nitrogen and adding mineral salts, blastospore concentration was 5.5 × 108 spores mL-1 cultured for 6 days using new formula. The germination of blastospores was not affected when suspension was placed at 4°C-30°C for 1-8 h. The number of lunate spores increased first and then decreased with the increase of initial blastospore concentration of E. vermicola Fxy121, medium concentration of blastospores suspension showed higher infectivity. The evF/evR primer pair successfully amplified the except fragment size of 522 bp from isolates of E. vermicola and from pine needles sprayed with blastospores suspension of E. vermicola. This study suggests that blastospores concentration of E. vermicola Fxy121 significantly affect the production of lunate spores and infectivity against B. xylophilus. The species-specific primers allowed fast and reliable detection of E. vermicola directly from tissues of pine trees sprayed and injected with spore suspension.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70019"},"PeriodicalIF":3.5,"publicationDate":"2025-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143692252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioprospecting Cold-Adapted Indole Acetic Acid-Producing Bacteria With Multifaceted Plant Growth Promoting Traits for Wheat Growth From Ghulkin Glacier, Pakistan. 对巴基斯坦古尔金冰川中具有促进小麦生长的多方面植物生长性状的冷适应吲哚乙酸生产细菌进行生物勘探。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-20 DOI: 10.1002/jobm.70017

Muhammad Irfan, Naseer Ahmad, Muhammad Rafiq, Jemma L Wadham, Christopher J Williamson, Abdul Haleem, Noor Hassan, Aamer Ali Shah, Fariha Hasan

{"title":"Bioprospecting Cold-Adapted Indole Acetic Acid-Producing Bacteria With Multifaceted Plant Growth Promoting Traits for Wheat Growth From Ghulkin Glacier, Pakistan.","authors":"Muhammad Irfan, Naseer Ahmad, Muhammad Rafiq, Jemma L Wadham, Christopher J Williamson, Abdul Haleem, Noor Hassan, Aamer Ali Shah, Fariha Hasan","doi":"10.1002/jobm.70017","DOIUrl":"https://doi.org/10.1002/jobm.70017","url":null,"abstract":"Agriculture in high altitude regions is challenged by low temperatures, and the use of chemical fertilizers turned hazardous in the long run. In addition, cost-effective, eco-friendly biofertilizers developed globally are less effective in cold regions due to temperature-sensitive mesophilic homologs. The current study evaluated the potential of a cold-adapted indole acetic acid (IAA)-producing bacterial consortium isolated from Ghulkin glacier, Hunza Valley, Pakistan, to promote the growth of the crop, wheat, under cold stress. Several IAA-producing bacteria were isolated and tested further for siderophore production, zinc-solubilizing activity, phosphate solubilizing activity, ability to utilize 1-aminocyclopropane-1-carboxylate and for nitrogen fixation. A microbial consortium was constructed and evaluated for its effectiveness in promoting wheat growth in pot experiments under ambient conditions (2°C-6°C at nighttime and 15°C-20°C at daytime) and with diverse soil and glacial flour matrices. The cold-adapted bacteria showed positive plant growth promoting attributes and with remarkable positive impacts on root length, shoot length, root and shoot fresh, and dry weight in pot trials in both garden soil and the glacial flour. An increase in wheat plant chlorophyll content was also observed in both pot experiments of glacial flour and garden soil. It was concluded that a multistrain bacterial consortium of Nocardiooides zeae GB3, Arthrobacter flavus GA9, and Pseudomonas sp. GB29 may have potential as an efficient biofertilizer for promoting mountainous crops, in the Gilgit Baltistan region, Pakistan.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70017"},"PeriodicalIF":3.5,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143669956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Context-Dependent Food Preferences and Comparative Decision-Making in Slime Mold Physarella oblonga. 黏菌长绒泡菌环境依赖的食物偏好和比较决策

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-17 DOI: 10.1002/jobm.70023

Hao-Yun Yin, Pin-Jhu Wang, Dong-Sheng Yang, Jui-Yu Chou

{"title":"Context-Dependent Food Preferences and Comparative Decision-Making in Slime Mold Physarella oblonga.","authors":"Hao-Yun Yin, Pin-Jhu Wang, Dong-Sheng Yang, Jui-Yu Chou","doi":"10.1002/jobm.70023","DOIUrl":"https://doi.org/10.1002/jobm.70023","url":null,"abstract":"Slime molds, despite their unicellular nature, exhibit complex behaviors and problem-solving abilities that have fascinated researchers. They are widely utilized as model organisms in behavioral studies. In this study, we examined the feeding preferences of the acellular slime mold Physarella oblonga when exposed to different yeast species as food sources. Our findings reveal significant preferences shown by P. oblonga for specific yeast strains. When Wickerhamomyces anomalus JYC2560 was another option, P. oblonga significantly favored Hanseniaspora osmophila JYC2504 (p < 0.05). Similarly, when Kazachstania exigua JYC2539 was another choice, P. oblonga exhibited a significant preference for Dekkera bruxellensis JYC2592 (p < 0.05). However, no significant preference was observed in other pair experiments. Further investigations highlighted variations in responses among different slime mold strains. Additionally, introducing a third yeast species as a decoy showed that P. oblonga preferred Hanseniaspora osmophila JYC2504 over Dekkera bruxellensis JYC2592 (p < 0.001), indicating a decoy effect. The preference can be changed if different yeast strains are used as decoys. Notably, this effect persisted even when the decoy yeast was fed to the slime mold in advance of the choice experiment, suggesting memory retention in slime molds. These findings contribute to our understanding of the intricate interactions between slime molds and their environment, emphasizing the diverse responses observed across different strains.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70023"},"PeriodicalIF":3.5,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143648444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Screening and Identification of Biocontrol Bacteria for Anthracnose in Mahonia eurybracteata Fedde. 蕨菜炭疽病生物防治菌的筛选与鉴定。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-11 DOI: 10.1002/jobm.70021

Qingyin Sun, Lisha Jiao, Qidan Chen, Huilin Shu, Xiyang Li, Zilong Li

{"title":"Screening and Identification of Biocontrol Bacteria for Anthracnose in Mahonia eurybracteata Fedde.","authors":"Qingyin Sun, Lisha Jiao, Qidan Chen, Huilin Shu, Xiyang Li, Zilong Li","doi":"10.1002/jobm.70021","DOIUrl":"https://doi.org/10.1002/jobm.70021","url":null,"abstract":"Mahonia eurybracteata exhibits characteristics related to heat dissipation, nutritional supplementation, and possesses antimicrobial and anticancer properties. Despite its widespread cultivation through artificial means, the detrimental impact of anthracnose on its growth has led to extensive reliance on chemical pesticides for disease control. Unscientific pesticide application and the use of outdated spraying techniques contribute to the presence of pesticide residues in both plants and the environment, thereby posing potential risks. This study utilized tissue isolation to segregate the pathogenic fungus causing anthracnose. The isolated strains, identified through 18S rRNA, were identified as Colletotrichum fructicola and Colletotrichum gloeosporioides. Koch's postulates were employed to confirm the pathogenicity of these strains. Simultaneously, soil samples from the rhizosphere of healthy M. eurybracteata were collected. Using the dilution plate method, 14 bacterial strains were isolated, and molecular biology techniques were employed for preliminary and subsequent screening via the agar confrontation method. Results indicated that selected strains of Pseudomonas gessardii and Pseudomonas fluorescens exhibited inhibitory effects on C. fructicola and C. gloeosporioides, respectively. Through in vitro detached leaf experiments, the biocontrol effectiveness of these two bacterial strains was validated. This study establishes a theoretical foundation for the development and application of antagonistic bacteria.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70021"},"PeriodicalIF":3.5,"publicationDate":"2025-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143604925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genome Mining and Antagonism of Stenotrophomonas geniculata MK-1, Against Peanut Foliage Fungus Diseases. 花生叶面真菌病嗜少养单胞菌MK-1的基因组挖掘及拮抗作用

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-10 DOI: 10.1002/jobm.70016

Taswar Ahsan, Mahnoor, Sulaiman Ali Alharbi, Maha A Alshiekheid, Amal Abdullah A Sabour, Ismail Khan, Mohammad Javed Ansari

{"title":"Genome Mining and Antagonism of Stenotrophomonas geniculata MK-1, Against Peanut Foliage Fungus Diseases.","authors":"Taswar Ahsan, Mahnoor, Sulaiman Ali Alharbi, Maha A Alshiekheid, Amal Abdullah A Sabour, Ismail Khan, Mohammad Javed Ansari","doi":"10.1002/jobm.70016","DOIUrl":"https://doi.org/10.1002/jobm.70016","url":null,"abstract":"Stenotrophomonas geniculata, a bacterium, has been recognized as an eco-friendly substitute for chemical fungicides in managing peanut foliar diseases, web blotch, and early leaf spot. Core genome and pan-genome analysis identified that strain MK-1 belongs to Stenotrophomonas geniculata, and nucleotide polymorphism (SNP) analysis confirmed that strain belongs to Stenotrophomonas maltophilia. The research revealed that S. geniculata MK-1 had a notable antagonistic impact on Peyronellaea arachidicola and Cercospora arachidicola and demonstrated a biocontrol efficacy of over 95% against peanut early leaf spot and web blotch disease. The nonredundant protein sequences (NR) database identified 4324 annotations related to S. geniculata, with 2682 genes similar to strain MK-1. The COG database categorized 3041 annotations into 22 functional groups, and 33 distinct metabolic pathways associated with 1851 Kyoto Encyclopedia of Genes and Genomes (KEGG) annotations. Most genes linked with metabolism are found in S. geniculata, with 380 genes related to carbohydrate metabolism and 44 genes related to secondary metabolite biosynthesis. The Carbohydrate-Active enZYmes (CAZy) database identified 194 annotations are linked to non-ribosomal synthesis of secondary metabolites. The Pathogen-Host Interactions (PHI) database showed reduced virulence in strain MK-1, while unaffected pathogenicity protein counts were 52. The MK-1 strain can produce antifungal siderophores secondary metabolites, non-ribosomal peptide synthetase (NRPS), and siderophores.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70016"},"PeriodicalIF":3.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143597002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Beneficial Tripartite Association of Pseudomonas Protegens, AM Fungi and Rice Promotes Growth Under Aerobic Conditions. 假单胞菌蛋白质、AM真菌和水稻有益的三方结合促进有氧条件下的生长。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-10 DOI: 10.1002/jobm.70014

Ekta Narwal, Kannepalli Annapurna, Jairam Choudhary, Yudh Vir Singh, Vijai Pal Bhadana

{"title":"Beneficial Tripartite Association of Pseudomonas Protegens, AM Fungi and Rice Promotes Growth Under Aerobic Conditions.","authors":"Ekta Narwal, Kannepalli Annapurna, Jairam Choudhary, Yudh Vir Singh, Vijai Pal Bhadana","doi":"10.1002/jobm.70014","DOIUrl":"https://doi.org/10.1002/jobm.70014","url":null,"abstract":"The positive impact of microbial inoculants on plants is well documented. In this study, we examined two rice varieties - PB 1509 (lowland) and Satyabhama (upland) - to investigate the effects of co-inoculating AMF and Pseudomonas protegens strain Ekn 03, under aerobic conditions. Co-inoculation with Pseudomonas protegens Ekn 03 significantly enhanced root mycorrhizal colonization in both varieties, while also increasing root surface area. In dually inoculated plants, shoot fresh weight, shoot dry weight, and root dry weight were 28.5%, 87.5%, and 22.2% higher, respectively, compared to non-inoculated control plants. Dually inoculated plants showed a 31.6% increase in chlorophyll content compared to control plants. Nitrate reductase (NR) activity was 28% higher in dually inoculated plants, comparable to those treated with RDF. Additionally, a significant correlation was observed between plant biomass, NR, and glutamine synthetase (GS) activity. This tripartite association improves phosphorus and nitrogen acquisition, indicating the potential for reduced chemical input use, with P. protegens Ekn 03 identified as an effective PGPR.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70014"},"PeriodicalIF":3.5,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143596999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Convergent Evolution of Coenzyme Metabolism in Methanosarcina mazei: Insights Into Primitive Life and Metabolic Adaptations. mazei甲烷藻辅酶代谢的趋同进化：对原始生命和代谢适应的见解。

IF 3.5 4区生物学

Journal of Basic Microbiology Pub Date : 2025-03-06 DOI: 10.1002/jobm.70015

Sivashanmuga Vadivel Saranya, Paulchamy Chellapandi

{"title":"Convergent Evolution of Coenzyme Metabolism in Methanosarcina mazei: Insights Into Primitive Life and Metabolic Adaptations.","authors":"Sivashanmuga Vadivel Saranya, Paulchamy Chellapandi","doi":"10.1002/jobm.70015","DOIUrl":"https://doi.org/10.1002/jobm.70015","url":null,"abstract":"The convergent evolution of coenzyme metabolism in methanogens provides critical insights into primitive life and metabolic adaptations. This study investigated the molecular evolution and functional dynamics of eight coenzymes and cofactors in Methanosarcina mazei, a model methanogen essential for methane production and energy conservation in anaerobic environments. Phylogenetic and genetic diversity analyses of the 706 protein sequences revealed conserved evolutionary trajectories interspersed with lineage-specific adaptations driven by gene duplication, horizontal gene transfer, and selective pressures. Key findings included the purifying selection of methanofuran (Tajima's D = -2.9589) and coenzyme A (Tajima's D = -2.8555), indicating the conservation of critical metabolic functions. The coenzyme B biosynthesis pathway showed balanced selection (Tajima's D = 2.38602), reflecting its evolutionary plasticity. Phylogenetic analyses linked coenzyme F420 biosynthetic enzymes closely to Methanosarcina horonobensis, while coenzyme F430 enzymes highlighted prokaryotic specialization distinct from their eukaryotes. Coenzyme M biosynthetic genes have demonstrated unique evolutionary connections with species across domains, such as Methanothermobacter thermautotrophicus and Gekko japonicus, emphasizing their broad adaptive significance. These evolutionary trajectories reveal how M. mazei optimized its metabolic pathways to thrive in extreme anaerobic environments, bridging ancient metabolic systems from the Last Universal Common Ancestor with contemporary ecological adaptations.","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70015"},"PeriodicalIF":3.5,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143573061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0