Journal of Basic Microbiology最新文献_第4页

Microbial Enzyme Production: Critical Bottlenecks and Integrated Engineering Solutions 微生物酶生产：关键瓶颈和综合工程解决方案。

IF 3.3 4区生物学

Journal of Basic Microbiology Pub Date : 2026-07-06 DOI: 10.1002/jobm.70180

Poulami Maji, Anannya Sengupta, Paramita Ghosh, Prashant Shukla

{"title":"Microbial Enzyme Production: Critical Bottlenecks and Integrated Engineering Solutions","authors":"Poulami Maji, Anannya Sengupta, Paramita Ghosh, Prashant Shukla","doi":"10.1002/jobm.70180","DOIUrl":"10.1002/jobm.70180","url":null,"abstract":"<div>\u0000 \u0000 Microbial enzymes are central to numerous industrial applications, yet their large-scale production remains constrained by persistent biological and process-level limitations. These include restricted secretion capacity, insufficient operational stability, scale-up inefficiencies, and downstream processing costs that can account for 45–65% of total manufacturing expenses. This review critically examines integrated genetic, physiological, and bioprocess engineering strategies developed to overcome these interconnected bottlenecks. Comparative analysis across major enzyme classes—cellulases, proteases, lipases, and amylases—reveals both universal constraints and enzyme-specific production challenges. Quantitative benchmarks indicate that multiplex genome engineering typically delivers 3–8-fold productivity improvements in optimized strains, while adaptive laboratory evolution enhances industrial robustness by 15–30%. Process intensification and continuous production approaches reduce processing times by 40–55%, albeit with increased capital requirements, and immobilization strategies enable 50–200 operational cycles with 65–85% retention of native activity. By synthesizing advances in strain engineering, secretion optimization, fermentation design, enzyme stabilization, and downstream integration within a techno-economic framework, this review highlights how coordinated interventions yield multiplicative gains over isolated strategies. Key future directions include systems-level characterization of secretion limits, AI-assisted enzyme stability engineering, and integrated manufacturing platforms. Collectively, these insights provide practical guidance for translating laboratory advances into economically viable microbial enzyme production systems.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 7","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148396345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Journal of Basic Microbiology. 7/2026 期刊信息：Journal of Basic Microbiology. 7/2026

IF 3.3 4区生物学

Journal of Basic Microbiology Pub Date : 2026-07-01 DOI: 10.1002/jobm.70182

引用次数: 0

Synergistic Biodegradation of Monocrotophos and Plant Growth Stimulation by Soil Bacterial Strains 土壤细菌菌株对单效磷的协同生物降解及对植物生长的促进作用。

IF 3.3 4区生物学

Journal of Basic Microbiology Pub Date : 2026-06-21 DOI: 10.1002/jobm.70178

Chinnadagudihundi Parashiva Harshitha, Hunasanalu Kuchela Narendra Kumar, Nagabhushana Chandra Mohana, Sreedharamurthy Satish

{"title":"Synergistic Biodegradation of Monocrotophos and Plant Growth Stimulation by Soil Bacterial Strains","authors":"Chinnadagudihundi Parashiva Harshitha, Hunasanalu Kuchela Narendra Kumar, Nagabhushana Chandra Mohana, Sreedharamurthy Satish","doi":"10.1002/jobm.70178","DOIUrl":"10.1002/jobm.70178","url":null,"abstract":"<div>\u0000 \u0000 Monocrotophos (MCP) is a widely used insecticide in agriculture, but its high toxicity poses significant environmental and health risks. The biodegradation of monocrotophos by indigenous microbes is crucial in reducing its toxicity. The present study was aimed at assessing plant growth- stimulating factors and the biodegradable ability of monocrotophos of two indigenous soil bacterial strains, CAB1 and SD8, isolated from monocrotophos-contaminated agricultural soils in Mysuru and Mandya, Karnataka, India. Biodegradation was evaluated using spectroscopy and chromatography, with substrate degradation confirmed by the disappearance of the main peak in HPLC profiles and intermediate metabolites identified through FTIR and LC-MS analyses. Molecular characterization identified CAB1 as Stenotrophomonas pavanii (OQ861163) and SD8 as Brevibacillus parabrevis (OQ881083). Results showed that the two bacterial strains utilized MCP as their sole carbon source and exhibited biodegradation rates of 88% and 90%, respectively, under optimal conditions. Results further showed that S. pavanii CAB1 and B. parabrevis SD8 produced ammonia, IAA, catalase, and cellulase, and demonstrated phosphorus and zinc solubilization. The degradation ability of CAB1 and SD8 is attributed to the presence of the opd gene and the opdA gene. Trimethyl phosphate, dimethyl phosphate, cyclohexanone, 2- cyclohexylidene, oxalic acid, and isohexyl pentyl esters were the non-toxic intermediates produced during the biodegradation of MCP. The strains S. pavanii CAB1 and B. parabrevis SD8 can be employed, alongside a microbial consortium formulation, for the detoxification of hazardous pollutants in contaminated soils and promotion of plant growth.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 6","pages":""},"PeriodicalIF":3.3,"publicationDate":"2026-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148295185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biofertilizers 生物肥料。

IF 3.3 4区生物学

Journal of Basic Microbiology Pub Date : 2026-06-21 DOI: 10.1002/jobm.70179

Erika Kothe

引用次数: 0

Microbial Shifts Across Endosphere and Rhizosphere as Strategy for Drought Adaptation in Shrub Ammopiptanthus mongolicus 沙冬青灌丛内、根际微生物迁移及其干旱适应策略

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-06-08 DOI: 10.1002/jobm.70177

Mingxia Shu, Yiling Zuo, Jingru Wang, Xueli He

{"title":"Microbial Shifts Across Endosphere and Rhizosphere as Strategy for Drought Adaptation in Shrub Ammopiptanthus mongolicus","authors":"Mingxia Shu, Yiling Zuo, Jingru Wang, Xueli He","doi":"10.1002/jobm.70177","DOIUrl":"10.1002/jobm.70177","url":null,"abstract":"<div>\u0000 \u0000 Although plant-associated microbes influence plant performance and ecological adaptability, knowledge of microbial variation between the root endosphere and rhizosphere of desert relict plants remains limited. Ammopiptanthus mongolicus, a relict species native to the hyper-arid deserts of northwestern China, was used to investigate microbial community structure across ecological niches and their soil ecological responses, aiming to elucidate adaptive plant–microbe symbioses and microbial distribution patterns. Root and rhizosphere soil samples were collected from Wuhai and Alxa in July 2021 to characterize microbial assemblages. Pronounced differences in community composition were observed between niches, with significantly higher species richness and α-diversity in the rhizosphere. PCoA indicated that niche type was the primary driver of microbial differentiation, outweighing site-level effects. Although more enriched taxa occurred in the rhizosphere, differentially abundant fungal taxa were far fewer than bacterial taxa. Co-occurrence network analysis showed that positive interactions dominated microbial associations, with the rhizosphere network exhibiting greater complexity, while endosphere fungal communities showed positive associations. Six low-abundance OTUs (< 0.1%) functioned as keystone taxa supporting network stability. AP, SOC, NH4+–N strongly shaped bacterial and fungal communities. Pathotrophs and saprotrophs dominated fungal guilds, whereas intracellular parasites and chemoheterotrophs prevailed among bacteria, with symbiotic fungi enriched in the endosphere.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148199049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Using Trichoderma spp. to Biological Control of Rhizoctonia solani Root Rot in Faba Beans 利用木霉对蚕豆根腐病的生物防治。

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-06-05 DOI: 10.1002/jobm.70175

Kamal A. M. Abo-Elyousr, Esmat F. Ali, Abeer S. Alqurashi, Ahmed M. K. Abdel-Aal, Nashwa M. A. Sallam

{"title":"Using Trichoderma spp. to Biological Control of Rhizoctonia solani Root Rot in Faba Beans","authors":"Kamal A. M. Abo-Elyousr, Esmat F. Ali, Abeer S. Alqurashi, Ahmed M. K. Abdel-Aal, Nashwa M. A. Sallam","doi":"10.1002/jobm.70175","DOIUrl":"10.1002/jobm.70175","url":null,"abstract":"<div>\u0000 \u0000 Root rot disease incited by Rhizoctonia solani, is one of destructive soil-borne diseases which constrains faba bean (Vicia faba L.) productivity. In the present research, pathogenicity of R. solani isolates was determined and potential of putative Trichoderma species were evaluated as a biological control in both in vitro as well greenhouse conditions. The most aggressive isolate of R. solani was determined with morphological characterization and sequencing of ITS rDNA. In vitro antagonistic potential of Trichoderma atroviride and Trichoderma asperellum against R. solani was evaluated by dual culture technique, and in greenhouse trials compared with a standard fungicide recommendation for comparison of efficacy. Both Trichoderma spp., strongly inhibited mycelia growth of R. solani in vitro, with inhibition ranging from over 85% for the most effective isolates. Trichoderma spp. applied to the soil under greenhouse conditions had significant influences on these trends significantly less damping-off and root rot disease incidence with an increase in the percentage of healthy surviving plants than the infected control. T. asperellum provided more efficient disease suppression than T. atroviride, whereas the chemical fungicide resulted in the greatest reduction of disease. Furthermore, Trichoderma-treated plants had higher activities of defense-related enzymes such as catalase, peroxidase and polyphenol oxidase which indicated the induction of systemic resistance. These results indicate that Trichoderma spp., mainly T. asperellum, can be a good alternative or supplement to chemical fungicides for the integrated disease management of R. solani-mediated root rot disease in V. faba L. under field conditions.\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-06-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148163409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A One-Step Dual Chromogenic Plate Assay for Reliable Screening of Naringinase-Producing Microorganisms 一步双显色板法可靠筛选柚皮酶产生微生物

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-06-01 DOI: 10.1002/jobm.70174

Satish V. Patil, Jitendra D. Salunkhe, Robert J. Mitchell

{"title":"A One-Step Dual Chromogenic Plate Assay for Reliable Screening of Naringinase-Producing Microorganisms","authors":"Satish V. Patil, Jitendra D. Salunkhe, Robert J. Mitchell","doi":"10.1002/jobm.70174","DOIUrl":"https://doi.org/10.1002/jobm.70174","url":null,"abstract":"<div>\u0000 \u0000 Naringinase is an industrially important enzyme system with applications in citrus debittering, aroma enhancement, and the production of bioactive flavonoid aglycones. Accurate identification of true naringinase-producing microorganisms remains challenging due to the dual-enzyme nature of naringinase, which requires coordinated α-L-rhamnosidase and β-D-glucosidase activities. Conventional screening methods based on naringin agar or single chromogenic substrates often generate false-positive results, as they fail to distinguish partial enzyme producers from microorganisms capable of complete naringin hydrolysis. In this study, a single-plate, one-step dual chromogenic screening assay was developed for reliable identification of true naringinase producers. The method employs p-nitrophenyl-α-L-rhamnopyranoside and X-gal to simultaneously detect α-L-rhamnosidase and β-glycosidase-like activities, producing distinct yellow and blue signals, respectively. The spatial separation of these chromogenic products enables unambiguous differentiation of microbial enzymatic profiles on a single agar plate. The assay was validated using bacterial strains with known enzymatic characteristics and further confirmed by spectrophotometric enzyme assays and thin-layer chromatography. Only isolates exhibiting both chromogenic responses demonstrated complete biotransformation of naringin to naringenin. A positive correlation between chromogenic halo diameter and measured enzyme activity was also observed. The proposed assay provides a simple, reliable, and scalable platform for high-throughput screening of naringinase-producing microorganisms in applied microbiology and biotechnology.</div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148128204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Issue Information: Journal of Basic Microbiology. 6/2026 期刊信息：Journal of Basic Microbiology. 6/2026

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-06-01 DOI: 10.1002/jobm.70176

引用次数: 0

Issue Information: Journal of Basic Microbiology. 6/2026 期刊信息：Journal of Basic Microbiology. 6/2026

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-06-01 DOI: 10.1002/jobm.70176

引用次数: 0

A One-Step Dual Chromogenic Plate Assay for Reliable Screening of Naringinase-Producing Microorganisms 一步双显色板法可靠筛选柚皮酶产生微生物

IF 2.7 4区生物学

Journal of Basic Microbiology Pub Date : 2026-06-01 DOI: 10.1002/jobm.70174

Satish V. Patil, Jitendra D. Salunkhe, Robert J. Mitchell

{"title":"A One-Step Dual Chromogenic Plate Assay for Reliable Screening of Naringinase-Producing Microorganisms","authors":"Satish V. Patil, Jitendra D. Salunkhe, Robert J. Mitchell","doi":"10.1002/jobm.70174","DOIUrl":"https://doi.org/10.1002/jobm.70174","url":null,"abstract":"<div>\u0000 \u0000 Naringinase is an industrially important enzyme system with applications in citrus debittering, aroma enhancement, and the production of bioactive flavonoid aglycones. Accurate identification of true naringinase-producing microorganisms remains challenging due to the dual-enzyme nature of naringinase, which requires coordinated α-L-rhamnosidase and β-D-glucosidase activities. Conventional screening methods based on naringin agar or single chromogenic substrates often generate false-positive results, as they fail to distinguish partial enzyme producers from microorganisms capable of complete naringin hydrolysis. In this study, a single-plate, one-step dual chromogenic screening assay was developed for reliable identification of true naringinase producers. The method employs p-nitrophenyl-α-L-rhamnopyranoside and X-gal to simultaneously detect α-L-rhamnosidase and β-glycosidase-like activities, producing distinct yellow and blue signals, respectively. The spatial separation of these chromogenic products enables unambiguous differentiation of microbial enzymatic profiles on a single agar plate. The assay was validated using bacterial strains with known enzymatic characteristics and further confirmed by spectrophotometric enzyme assays and thin-layer chromatography. Only isolates exhibiting both chromogenic responses demonstrated complete biotransformation of naringin to naringenin. A positive correlation between chromogenic halo diameter and measured enzyme activity was also observed. The proposed assay provides a simple, reliable, and scalable platform for high-throughput screening of naringinase-producing microorganisms in applied microbiology and biotechnology.</div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"66 6","pages":""},"PeriodicalIF":2.7,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148128150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0