Journal of Basic Microbiology最新文献

{"title":"Cover: Journal of Basic Microbiology. 2/2025","authors":"","doi":"10.1002/jobm.202570003","DOIUrl":"https://doi.org/10.1002/jobm.202570003","url":null,"abstract":"<p><b>Cover illustration:</b></p><p>Actinomycetes isolated from a metal rich environment in a former uranium mining area near Ronneburg, Thuringia. The strains show high metal resistance.</p><p>(Photo: Götz Haferburg, Friedrich-Schiller-University, Jena, Germany)\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 2","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.202570003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389392","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultivation Strategies to Improve Chlamydomonas reinhardtii Growth and Recombinant Mcherry Expression.","authors":"Jassiara da Silva Pessoa, Bruno Guzzo da Silva, Ednilson Donisete de França Júnior, Isac José da Silva Filho, João Vitor Dutra Molino, João Carlos Monteiro de Carvalho, Livia Seno Ferreira-Camargo","doi":"10.1002/jobm.70006","DOIUrl":"https://doi.org/10.1002/jobm.70006","url":null,"abstract":"<p><p>Chlamydomonas reinhardtii is a promising model microalga for recombinant molecules production. Nonetheless, low yield is a challenge for its industrial use. This work investigated the influence of ammonium chloride (NH₄Cl) concentration and temperature on the growth of transgenic C. reinhardtii expressing the fluorescent protein mCherry on a laboratory scale. A Central Composite Rotatable Design was used to establish the cultivation conditions. NH₄Cl concentrations ranging from 400 to 647.49 mg/L and temperatures between 25°C and 32.1°C resulted in maximum values of cell concentration and mCherry fluorescence. Lower temperatures (15°C-17°C) were found to be more suitable for the accumulation of total soluble proteins. These results demonstrate that cultivation conditions can positively affect C. reinhardtii growth, with a range of conditions that can be used. Unlike genetic approaches, this study provides a solution to enhance both growth and recombinant protein expression in C. reinhardtii. These findings pave the way for scaling up the use of C. reinhardtii as a biofactory in industry and can be applied to other microalgal systems.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70006"},"PeriodicalIF":3.5,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the Red Dye From Arthrinium phaeospermum and Its Application in Wood Dyeing.","authors":"Mengqi Wu, Taize Song, Tingwei Pang, Pengyan Zhuang, Jiashun Niu, Yalan Li, Jianping Sun","doi":"10.1002/jobm.70001","DOIUrl":"https://doi.org/10.1002/jobm.70001","url":null,"abstract":"<p><p>The aim of this study was to investigate the production, stability and applicability of dyes produced by filamentous fungi isolated from wood. First, the effect of culture conditions on fungal growth and dye production was investigated. The dyes were experimentally studied for solubility and stability and chemically characterized by FT-IR and UPLC-Q-EXACTIVE-MS. Finally, the dye was used to evaluate its industrial applicability by staining a variety of woods. The results showed that the filamentous fungus Arthrinium phaeospermum was able to produce red water-soluble pigments, which were thermally and ultraviolet stable and remained stable in pH 1-9. Chemical analysis showed that the red dye contained the chromogenic substances bostrycin and about 7.01% of griseofulvin. Dyeing experiments showed that the red dye was able to give the wood a red color and a natural grain. The results of various experiments indicate that A. phaeospermum has the potential to produce dyes for use in the wood dyeing and textile industries.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70001"},"PeriodicalIF":3.5,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143398979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Effects of Bacillus strains applied Via Seed Biopriming and Soil Drenching Applications on the Morpho-Physiological and Transcriptional Aspects of Cotton","authors":"Tahir Mahmood,&nbsp;Anam Moosa,&nbsp;Faisal Zulfiqar,&nbsp;Muhammad Naveed Aslam,&nbsp;Hongwei Zhao,&nbsp;Meisam Mohammadi,&nbsp;Tolga İzgü,&nbsp;Taner Bozkurt,&nbsp;Temoor Ahmed,&nbsp;Doaa Bahaa Eldin Darwish","doi":"10.1002/jobm.202400665","DOIUrl":"10.1002/jobm.202400665","url":null,"abstract":"<div>\u0000 \u0000 <p>Biofertilizers are considered as a sustainable solution for promoting the growth and productivity of crops while reducing the dependence on chemical fertilizers. There is a growing need for the sustainable agricultural solutions to lessen the reliance on chemical fertilizers; thus, evaluating <i>Bacillus</i> strains as biofertilizers for cotton growth promotion can support eco-friendly and economically viable crop production. Therefore, the growth promoting potential of endophytic <i>Bacillus altitudinis</i> strain TM22 and <i>B. atrophaeus</i> strain MCM61 applied as soil drenching or seed treatment, was evaluated on cotton cv<i>. '</i>SS32'. <i>In vitro</i>, the qualitative assay both TM22 and MCM61 showed proteolytic, amylolytic, lipolytic, cellulolytic, and chitinolytic activity. TM22 and MCM61 strains also demonstrated the ability to produce siderophores, indole 3-acetic acid (IAA), and phosphate solubilization. In the pot experiment, seed biopriming with TM22 and MCM61 had better performance regarding plant growth and biomass, photosynthetic pigments, stomatal conductance, and relative leaf water contents than the soil drenching application. The gene expression analysis of growth hormones-related genes demonstrated that TM22 and MCM61 showed an upregulated expression of <i>ARF1, ARF18, EXP6, IAA9, GIB1b</i>, and <i>CKX6</i> while <i>ERF</i> and <i>ERF17</i> genes were downregulated. Overall, these findings suggest that seed biopriming with <i>B. altitudinis</i> TM22 and <i>B. atrophaeus</i> MCM61 is an effective method with the potential to enhance the biomass of cotton.</p></div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143364775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Carbon-Based Nanomaterials Alter the Behavior and Gene Expression Patterns of Bacteria.","authors":"Shima Afrasiabi, Alireza Partoazar, Ramin Goudarzi, Ahmad Reza Dehpour","doi":"10.1002/jobm.202400545","DOIUrl":"https://doi.org/10.1002/jobm.202400545","url":null,"abstract":"<p><p>One of the most dangerous characteristics of bacteria is their propensity to form biofilms and their resistance to the drugs used in clinical practice today. The total number of genes that can be categorized as virulence genes ranges from a few hundred to more than a thousand. The bacteria employ a variety of mechanisms to regulate the expression of these genes in a coordinated manner during infection. The search for new agents with anti-virulence capacity is therefore crucial. Nanotechnology provides safe platforms for targeted therapies to combat a broad spectrum of microbial infections. As a new class of innovative materials, carbon-based nanomaterials (CBNs), which include carbon dots, carbon nanotubes, graphene, and fullerenes can have strong antibacterial activity. Exposure to CBNs has been shown to affect bacterial gene expression patterns. This study investigated the effect of CBNs on the repression of specific genes related to bacterial virulence/pathogenicity.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e2400545"},"PeriodicalIF":3.5,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Stress-Induced Response and Adaptation Mechanisms in Bacillus licheniformis PSKA1 Exposed With Abiotic and Antibiotic Stresses","authors":"Arihant Jayawant Kadapure,&nbsp;Nagarjuna Prakash Dalbanjan,&nbsp;Praveen Kumar S.K.","doi":"10.1002/jobm.202500016","DOIUrl":"10.1002/jobm.202500016","url":null,"abstract":"<div>\u0000 \u0000 <p>Soil ecosystems consist of diverse microbial communities with great potential for ecological and biotechnological applications. These communities encounter various abiotic stresses, which expedite the activation of transient overexpression of heat shock proteins (HSPs). In the present study, a soil bacterium was isolated and identified as <i>Bacillus licheniformis</i> strain PSK.A1, and its growth parameters were optimized before exposing it to heat, salt, pH, and antibiotic stress conditions. Comparative protein expression was analyzed using SDS-PAGE, protein stabilization via protein aggregation assays, and survival through single spot dilution and colony-counting methods under various stress conditions. The pre-treatment of short stress dosage showed endured overall tolerance of bacterium to lethal conditions, as evidenced by moderately enhanced total soluble intracellular protein content, better protein stabilization, comparatively over-expressed HSPs, and relatively enhanced cell survival. The findings highlighted that cells grown under optimal conditions were more susceptible to lethal environments than stressed cells, with their enhanced tolerance linked to the overexpression of 20 distinct HSPs of 17–91 kD. These insights offer the potential for developing strategies to enhance microbial resilience for various applications including bacterial bioprocessing, bio-remediation, and infectious disease management.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 3","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and Ultrastructural Impact of Metarhizium anisopliae and Metarhizium robertsii on Myllocerus subfasciatus","authors":"Udhayakumar Monisha,&nbsp;Pagalahalli Sankaran Shanmugam,&nbsp;Marimuthu Murugan,&nbsp;Subramanian Jeyarani,&nbsp;Nandagopal Geetha,&nbsp;Thulasy Srinivasan,&nbsp;Angappan Suganthi,&nbsp;Rajasekaran Raghu,&nbsp;Kabirdoss Indhumathi,&nbsp;Rajendran Yamini,&nbsp;Murugesan Naveen,&nbsp;Perumal Vivekanandhan","doi":"10.1002/jobm.70000","DOIUrl":"10.1002/jobm.70000","url":null,"abstract":"<div>\u0000 \u0000 <p>The ash weevil, <i>Myllocerus subfasciatus</i>, is a significant insect pest that infests brinjal. Both the adults and grubs feed on the leaves and roots, respectively, leading to considerable yield loss. The subterranean habits of the larvae limit the effectiveness of insecticide applications, necessitating the implementation of integrated pest management programs that utilize entomopathogenic fungi. This investigation aimed to identify potential <i>Metarhizium</i> species against ash weevil larvae through scanning electron microscopy (SEM) and histopathology. The ash weevils were mass-cultured on brinjal plants under insect-proof conditions. Eleven <i>Metarhizium</i> sourced from the departmental repository were subjected to pathogenicity tests on second-instar ash weevil larvae, revealing that a concentration of 1 × 10⁶ conidia/mL was optimal for SEM and histological studies. Among the 11 <i>Metarhizium</i> strains examined, the TNAU ENTMA TDM 8 strain produced spores measuring 5.8 µm in length and 2.4 µm in width in both potato dextrose agar (PDA) medium and larvae. SEM analysis indicated that the mycelial adherence and penetration of <i>Metarhizium</i> were most pronounced in the larvae 5 days post inoculation (DPI). Histopathological investigations demonstrated that the TNAU ENTMA TDM 8 strain caused degradation of fat bodies and hemocytes at 3 DPI, and complete body distortion at 7 DPI, while the untreated control exhibited no such effects. The <i>M. robertsii</i> strain TNAU ENTMR GYU 1 displayed slower infectivity compared to the <i>M. anisopliae</i> strains. The TNAU ENTMA TDM 8 strain was determined to be the most effective against <i>M. subfasciatus</i> larvae and can be utilized for managing ash weevil populations.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143080126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adaptive Development of Soil Bacterial Communities to Ecological Processes Caused by Mining Subsidence.","authors":"Yan Yu, Yuanjia Li, Jiangning Zhou, Jie Zhang, Wen Li","doi":"10.1002/jobm.70002","DOIUrl":"https://doi.org/10.1002/jobm.70002","url":null,"abstract":"<p><p>Subsidence from coal mining is a major environmental issue, causing significant damage to soil structure. Soil microorganisms, highly sensitive to environmental changes, adapt accordingly. This study focused on four areas of the Burdai coal mine: a non-subsidence area (CK), half-yearly (HY), 1-year (OY), and 2-year (TY) subsidence areas. Using high-throughput sequencing and molecular ecological network analysis, we examined soil microbial community diversity and structure across these zones, exploring microbial community assembly and functional predictions. Results showed that compared to the control, subsidence areas experienced reduced soil water content, organic matter, available phosphorus, and alkaline nitrogen, with the lowest levels observed at 1 year. These values began to rise after 1 year, suggesting natural recovery after subsidence stabilized. Microbial communities were closely related to soil organic matter, water content, and alkaline nitrogen. At the 1-year mark, soil property changes significantly reduced microbial diversity, which then began to recover after 2 years. The microbial network during 1-year subsidence was simpler, with 102 nodes, 179 edges, and an average degree of 3.51, indicating that early subsidence was unstable, and the microbial community was still adapting. By 1 year, community structure and interactions had begun to stabilize. Stochastic processes played a key role in microbial variability during short-term subsidence.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e70002"},"PeriodicalIF":3.5,"publicationDate":"2025-01-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065828","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Tolerance to NSAIDs in Actinobacteria From a Mexican Volcano Crater: Genomics and Bioremediation Potential","authors":"Claudia Soria-Camargo,&nbsp;Lorna Catalina Can-Ubando,&nbsp;Gauddy Lizeth Manzanares-Leal,&nbsp;Ayixon Sánchez-Reyes,&nbsp;Sonia Dávila-Ramos,&nbsp;Ramón Alberto Batista-García,&nbsp;Ninfa Ramírez-Durán","doi":"10.1002/jobm.202400772","DOIUrl":"10.1002/jobm.202400772","url":null,"abstract":"<div>\u0000 \u0000 <p>Non-steroidal anti-inflammatory drugs (NSAIDs) are emerging contaminants that pose significant health and environmental risks due to their persistence, including their presence in drinking water. Bioremediation, particularly through microorganisms such as actinobacteria, offers a sustainable approach to mitigate these pollutants. Actinobacteria from poly-extreme environments exhibit unique genetic and metabolic adaptations, enabling resistance to and degradation of various contaminants. This study aimed to evaluate the tolerance of actinobacteria to NSAIDs and conduct a genomic analysis of a selected strain. Actinobacteria were isolated from the crater of the Chichonal volcano [Chiapas, Mexico), resulting in 16 isolates. Among these, <i>Micrococcus luteus</i> P8SUE1, <i>Micrococcus yunnanensis</i> P9AGU1, and <i>Kocuria rhizophila</i> P1AGU3 demonstrated tolerance to diclofenac, ibuprofen, and paracetamol at concentrations of 1 ppm, 10 ppm, and 100 ppm, respectively. Whole-genome sequencing of <i>M. yunnanensis</i> P9AGU1 identified genes linked to the degradation of aromatic compounds and adaptations to extreme environmental conditions, highlighting its potential for bioremediation applications.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143065830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metacaspases-Like Proteases of Trichomonas vaginalis: In Silico Identification and Characterization.","authors":"Julio César Torres-Romero, María Elizbeth Alvarez-Sánchez, Marcos Morales-Reyna, Andrea Bellavista-Caballero, Rodrigo Arreola, Leidi C Alvarez-Sánchez, Julio Lara-Riegos","doi":"10.1002/jobm.202400786","DOIUrl":"https://doi.org/10.1002/jobm.202400786","url":null,"abstract":"<p><p>Metacaspases (MCA), are cysteine-dependent proteases closely related to caspases. In protozoa, MCA plays an important role in programmed cell death (PCD). In Trichomonas vaginalis, a kind of PCD that resembles apoptosis has been described, but the activators of this mechanism have not been demonstrated. We performed a genome-wide in silico analysis in the T. vaginalis database using consensus MCA domains. A total of 15 protein annotations for MCA-like sequences were retrieved. Only 7/15 (TvMCA1-6 and TvMCA9) of the sequences were annotated as putative MCA and exhibited a similar range of amino acid length in comparison to the consensus sequences used for the query. By in silico analysis, we found that they are thermostable, hydrophilic proteins with molecular weights ranging from 27 to 33 KDa and their theoretical isoelectric points are in a 5.08-8.57 range. The phylogenetic analysis showed the similarity of conserved motifs for the predicted TvMCA proteins. 3D structure prediction by homology modeling demonstrated that TvMCA proteins show a similar conformation to crystallized MCA proteins. Taken together, our results indicate that these trichomonad proteins have conserved sequences like MCA proteins and suggest that they may be responsible for proteolytic activity during a PCD-like mechanism in this parasite.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e2400786"},"PeriodicalIF":3.5,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}