Journal of Basic Microbiology最新文献

{"title":"Special Focus: Antibiotics and Antibiotic Resistance Crisis","authors":"Erika Kothe","doi":"10.1002/jobm.202500050","DOIUrl":"10.1002/jobm.202500050","url":null,"abstract":"","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 3","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143046868","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Combination of α-Fe2O3 NP and Trichoderma sp. Improves Antifungal Activity Against Fusarium Wilt","authors":"Sushma Sharma,&nbsp;Poonam Kumari,&nbsp;Mamta Shandilya,&nbsp;Sapna Thakur,&nbsp;Kahkashan Perveen,&nbsp;Imran Sheikh,&nbsp;Zubair Ahmed,&nbsp;Riyaz Sayyed,&nbsp;Andrea Mastinu","doi":"10.1002/jobm.202400613","DOIUrl":"10.1002/jobm.202400613","url":null,"abstract":"<p>Soil-borne plant pathogens are the most damaging pathogens responsible for severe crop damage. A conventional chemotherapy approach to these pathogens has numerous environmental issues, while biological control agents (BCAs) are less promising under field conditions. There is an immediate need to develop an integrated strategy for utilizing nanoparticles and biocontrol to manage soil-borne pathogens, such as <i>Fusarium</i> wilt, effectively. Simulation of BCA metabolites to nanoparticle biocontrol metabolites is considered the most effective biocontrol approach. Combining Fe₂O₃ nanoparticles and <i>Trichoderma</i> in nursery and field conditions manages pathogens and increases plant growth characteristics. The present study evaluated the commercial biocontrol strains and the use of NPFe in combination with <i>Trichoderma harzianum</i> to enhance the biocontrol potential of <i>T. harzianum</i> against soil-borne pathogens. The effectiveness of (NPFe + <i>T. harzianum</i>) was evaluated under in vitro conditions where combination was found most effective upto (87.63%) mycelial growth inhibition of pathogen and under field conditions lowest pooled <i>Fusarium</i> wilt incidence (19.54%) was recorded. Nanocomposites are beneficial for agricultural sustainability and environmental safety by upregulating the expression of genes linked to these processes, Fe NPs can activate plant defense mechanisms and increase plant resistance to pathogenic invasions. Additionally, as iron is a necessary component for plant growth and development, Fe NPs promote better nutrient uptake.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.202400613","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005841","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development, Shelf Stability, and In-Vitro Evaluation of Liquid Bacterial Inoculant Acinetobacter lwoffii Strain PAU_31LN","authors":"Jagjot Kaur,&nbsp;Gulab Pandove,&nbsp;Vineet Kumar,&nbsp;Amal Abdullah A. Sabour,&nbsp;Maha Alshiekheid","doi":"10.1002/jobm.202400617","DOIUrl":"10.1002/jobm.202400617","url":null,"abstract":"<div>\u0000 \u0000 <p><i>Acinetobacter</i> has been recognized as a versatile plant growth promoting (PGP) rhizobacteria (PGPR) that produce multiple PGP traits. The present study was conducted to formulate an efficient and stable liquid bacterial inoculant (LBI) of <i>Acinetobacter lwoffii</i> strain PAU_31LN. In the current investigation, total 16 endophytic bacteria were isolated from cotton leaves and evaluated for plant growth-promoting features such as production of phytohormones, mineral solubilization, siderophore production, hydrogen cyanide (HCN) production, and 1-aminocyclopropane-1-carboxylate (ACC) deaminase activity. The leaf endophytic bacteria designated as 31LN was found promising for all the PGP traits and it was identified as <i>A. lwoffii</i> strain PAU_31LN by 16S rRNA gene sequencing. For the development of LBI of <i>A. lwoffii</i> strain PAU_31LN, 4.5 g/L yeast extract, 5 g/L NaCl, 5 g/L peptone, and 12.5 mM food-grade trehalose was optimized as appropriate medium composition using response surface methodology (RSM) and Box–Behnken design. Further, the viability of <i>A. lwoffii</i> strain PAU_31LN in the optimized formulation was observed as 1.1 folds higher over the control after 180 days of storage at room temperature. Moreover, nonsignificant variation was recorded in the functional traits of 180 days old LBI of <i>A. lwoffii</i> strain PAU_31LN and freshly prepared LBI. The in-vitro plant growth parameters such as length and seed vigor index of 7-day-old cotton seedlings were enhanced by the seed bio-priming with LBI of <i>A. lwoffii</i> strain PAU_31LN over the control. The results of the present study signify the importance of endophytes and statistical methods to formulate prominent LBI.</p></div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143005837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Synthesis of Silver-Doped ZnO Nanoparticles From Adiantum venustum D. Don (Pteridaceae): Antimicrobial and Antioxidant Evaluation","authors":"Adil Noor,&nbsp;Nisar Ahmad,&nbsp;Amjad Ali,&nbsp;Musarat Ali,&nbsp;Majid Iqbal,&nbsp;Muhammad Nauman Khan,&nbsp;Marzia Batool Laila,&nbsp;Syed Nasar Shah,&nbsp;Alevcan Kaplan,&nbsp;Sezai Ercişli,&nbsp;Mohamed Soliman Elshikh","doi":"10.1002/jobm.202400543","DOIUrl":"10.1002/jobm.202400543","url":null,"abstract":"<div>\u0000 \u0000 <p>One of the main difficulties in nanotechnology is the development of an environmentally friendly, successful method of producing nanoparticles from biological sources. Silver-doped zinc oxide nanoparticles (Ag-ZnO NPs), with antibacterial and antioxidant properties, were produced using <i>Adiantum venustum</i> extract as a green technique. Fresh <i>A. venustum</i> plants were gathered, then their bioactive elements were extracted with cold water and processed into nanoparticles. The main goal was to develop Ag-ZnO NPs (nanoparticles) for medical applications, especially with regard to their antifungal and antibacterial properties. Pathogens such as <i>Fusarium oxysporum</i>, <i>Escherichia coli</i>, and <i>Staphylococcus aureus</i> were tested against the synthesized nanoparticles. While FTIR spectroscopy revealed functional groups, X-ray diffraction validated the crystalline structure. The scanning electron microscope analysis revealed that the Ag-ZnO NPs had an average size of 30.16 nm and an irregular shape. Additionally, energy dispersive X-ray analysis) confirmed the elemental composition. The bioactive compounds present in <i>A. venustum</i> significantly stabilized the nanoparticles. Strong antioxidant and antibacterial activity of the Ag-ZnO nanoparticles was demonstrated. In particular, this work shows that the Ag-ZnO nanoparticles produced by green synthesis could be used in biomedical drug delivery and therapy.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142978483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent Advancements and Strategies for Omega-3 Fatty Acid Production in Yeast","authors":"Priya Kumari,&nbsp;Juhi Sharma,&nbsp;Prashant Khare","doi":"10.1002/jobm.202400491","DOIUrl":"10.1002/jobm.202400491","url":null,"abstract":"<div>\u0000 \u0000 <p>Recently, the biosynthesis of omega-3 fatty acids (ω3 FAs) in yeast has witnessed significant advancements. Notably, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) play crucial roles in overall human growth, encompassing neurological development, cardiovascular health, and immune function. However, traditional sources of ω3 FAs face limitations such as environmental concerns. Yeast, as a genetically tractable organism, offers a promising alternative for its sustainable production. Recent advancements and strategies in yeast through metabolic engineering led to significant improvements in ω3 FA production, including the optimization of metabolic pathways, enhancement of precursor supplies, and manipulation of gene expression. Moreover, innovative bioprocess approaches, such as fermentation conditions and bioreactor design, have been devised to further maximize its yields. This review aims to comprehensively summarize recent strategies in ω3 FA production within yeast systems, highlighting their contribution to meeting global ω3 FA demand while mitigating environmental impact and ensuring food security.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 3","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142970949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Issue Information: Journal of Basic Microbiology. 1/2025","authors":"","doi":"10.1002/jobm.202570002","DOIUrl":"https://doi.org/10.1002/jobm.202570002","url":null,"abstract":"","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.202570002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143112478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cover: Journal of Basic Microbiology. 1/2025","authors":"","doi":"10.1002/jobm.202570001","DOIUrl":"https://doi.org/10.1002/jobm.202570001","url":null,"abstract":"<p><b>Cover illustration:</b></p><p>Biocontrol to combat <i>Ganoderma</i> using soil-derived fungal isolates identified strains of <i>Talaromyces</i> and <i>Penicillium</i> sp., which possess additional plant growth-promoting traits. The micrograph shows conidiophore bearing conidia of this new biocontrol agent.</p><p>(Photo: Muhammad Salahudin Kheirel Anuar, Department of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, Serdang, Selangor, Malaysia)\u0000\u0000 <figure>\u0000 <div><picture>\u0000 <source></source></picture><p></p>\u0000 </div>\u0000 </figure></p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 1","pages":""},"PeriodicalIF":3.5,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/jobm.202570001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143112479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autophagy Activated by Atg1 Interacts With Atg9 Promotes Biofilm Formation and Resistance of Candida albicans.","authors":"Yun Huang, Shenjun Yu, Siqi Liu, Xiao Zhao, Xueyi Chen, Xin Wei","doi":"10.1002/jobm.202400603","DOIUrl":"https://doi.org/10.1002/jobm.202400603","url":null,"abstract":"<p><p>Autophagy regulates the development of Candida albicans (C. albicans) biofilms and their sensitivity to antifungals. Atg1, a serine/threonine protein kinase, recruits autophagy-related proteins for autophagosome formation. Atg9, the only transmembrane protein, is phosphorylated by Atg1 during autophagy. The specific roles of Atg1 and Atg9 in biofilm formation and resistance of C. albicans remain unclear. The study used RT-qPCR and Western blotting to assess the correlation between Atg1, Atg9 and biofilm formation, XTT reduction assays to evaluate biofilm formation and antifungal resistance, commercial kits to detect reactive oxygen species (ROS), mitochondrial membrane potential (MMP), and autophagy activity, transmission electron microscopy (TEM) to study the morphological changes, protein-protein interaction (PPI) analysis to analyze the interaction between Atg1 and Atg9. Results demonstrated that Atg1 and Atg9 were highly expressed in biofilms than planktonic cells. Biofilm formation, antifungal resistance, MMP and autophagy activity decreased and ROS increased in atg1Δ/Δ and atg9Δ/Δ. TORC1 inhibition with rapamycin rescued the reduced biofilm formation of atg1Δ/Δ and increased antifungal resistance of atg1Δ/Δ and atg9Δ/Δ. PPI analysis and TEM observation indicated that Atg1 interacted with Atg9, which was certified by RT-qPCR and Western blotting. This study suggested that Atg1 interacts with Atg9, activates the autophagy regulating the formation and sensitivity of C. albicans biofilms.</p>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":" ","pages":"e2400603"},"PeriodicalIF":3.5,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142894537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Discovery of Novel ER-Localized Cellobiose Transporters Involved in Cellulase Biosynthesis in Trichoderma reesei","authors":"Haiyan Wang,&nbsp;Xiaotong Shi,&nbsp;Liujie Huo,&nbsp;Jing Tu,&nbsp;Chengcheng Li,&nbsp;Fu-Gen Wu,&nbsp;Fengming Lin","doi":"10.1002/jobm.202400573","DOIUrl":"10.1002/jobm.202400573","url":null,"abstract":"<div>\u0000 \u0000 <p>Sugar transporters are of great importance in sensing and transporting varied sugars for cellulase biosynthesis of lignocellulolytic fungi. Nevertheless, the function and the relevant mechanism of sugar transporters in fungal cellulase biosynthesis remain to be explored. Here, putative maltose transporters Mal1, Mal2, Mal3, Mal4, and Mal5 in <i>Trichoderma reesei</i> were investigated. Despite that only the transcriptional abundance of <i>Mal1</i> was upregulated under cellulase-generating condition, the individual deletion of <i>Mal1</i>, <i>Mal2</i>, <i>Mal3</i>, <i>Mal4</i>, and <i>Mal5</i> all impaired cellulase biosynthesis. The possible reason for this is that the individual knockout of <i>Mal2</i>, <i>Mal3</i>, <i>Mal4</i>, and <i>Mal5</i> resulted in no gene expression of <i>Mal1</i> at 24 h during the cellulase production. The transcriptional analysis showed that the absence of these transporters noticeably inhibited cellulase genes at 24 h, which was then relieved. Interestingly, the individual missing of these maltose transporters significantly retarded the cellular consumption of cellobiose, rather than maltose, and they were distributed in cytoplasm, largely in endoplasmic reticulum (ER). These findings manifested that these putative maltose transporters may be in fact endomembrane cellobiose transporters, influencing fungal cellulase generation probably through Mal1 at the early stage. This research advances the knowledge of endomembrane sugar transporters in fungal cellulase biosynthesis.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142894538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodegradation of Organophosphorus Insecticides by Bacillus Species Isolated From Soil","authors":"Subramanian Muthukumaravel,&nbsp;Balakrishnan Sivalaxmi,&nbsp;Shriram Ananganallur Nagarajan,&nbsp;Natesan Sivakumar,&nbsp;Ashwani Kumar,&nbsp;Sugeerappa Laxmanappa Hoti","doi":"10.1002/jobm.202400597","DOIUrl":"10.1002/jobm.202400597","url":null,"abstract":"<div>\u0000 \u0000 <p>This study investigates the biodegradation of methyl parathion, an organophosphate pesticide used in paddy fields. Microbial degradation transforms toxic pesticides into less harmful compounds, influenced by the microbial community in the soil. To isolate different microbial colonies, soil samples from an organophosphorus-treated groundnut field were plated on nutrient agar and MSM with 1% glucose and 0.25 mM methyl parathion. Biodegradation efficiency was determined by estimating the OP hydrolase enzyme activity spectrophotometrically. HPLC was used to quantify residual methyl parathion concentrations in the culture medium. The identified isolate effectively degraded methyl parathion in MSM with 0.25 mM methyl parathion which showed peak hydrolase activity (2.02 µmol/min/mg) after 96 h of incubation and the residual methyl parathion level was determined as 6.2 µmol by HPLC quantification. The efficient isolate was identified as <i>Bacillus cereus</i> by using a 16S rRNA molecular marker and the sequence was subjected to MEGA11 phylogenetic tree construction. The results show that the SM6 clade shared with <i>B. cereus</i> 16S rRNA sequence. <i>B. cereus</i> (SM6) showed substantial enzyme activity and the specific reported opdA gene-coded protein is involved in ATP hydrolysis. This OP hydrolase makes it a strong candidate for bioremediation of methyl parathion. Molecular analysis suggested that the <i>opdA</i> gene, likely chromosomally located, plays a key role in degradation, with potential involvement of the “Cell division protein FtsK” gene responsible for hydrolase activity. Organophosphorus compounds, widely used in agriculture, pose environmental concerns due to their persistence. This study focuses on isolating pesticide-degrading bacteria to expedite bioremediation, aiming for efficient degradation. This study highlights the cross-adaptation phenomenon, where <i>B. cereus</i> strains degrade similar compounds, improving bioremediation strategies.</p>\u0000 </div>","PeriodicalId":15101,"journal":{"name":"Journal of Basic Microbiology","volume":"65 4","pages":""},"PeriodicalIF":3.5,"publicationDate":"2024-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142877036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}