Function and Regulatory Mechanism of a Two-Component Signal Transduction System cgtRS5 in Corynebacterium glutamicum

IF 2.7 4区生物学 Q2 MICROBIOLOGY

Journal of Basic Microbiology Pub Date : 2025-07-27 DOI:10.1002/jobm.70085

Zhaoxin Peng, Can Chen, Runge Xu, Ruobing Wang, Ruixue Wang, Jinshuo Liu, Ting Zou, Meiru Si, Tao Su

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Abstract

The putative two-component system (TCS) CgtRS5, encoded by the ncgl2572–ncgl2573 gene cluster, plays a critical role in environmental adaptation in Corynebacterium glutamicum. Comparative transcriptomic analysis between the wild-type strain and a ΔcgtRS5 mutant revealed three upregulated and 107 downregulated genes in the mutant. Among these, genes involved in iron metabolism (ncgl1959), aromatic compound degradation (ncgl2320), and drug resistance (cssR) were significantly downregulated. Phenotypic assays demonstrated that the ΔcgtRS5 mutant exhibited impaired growth in media supplemented with the divalent form of iron (Fe²⁺) or the alkylating agent iodoacetamide (IAM), but showed no significant differences under benzoate, resorcinol, or antibiotic stress. These results suggest that CgtRS5 specifically regulates the stress response to iron and IAM in C. glutamicum, providing new insights into TCS-mediated regulatory mechanisms in this organism.

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谷氨酸棒状杆菌双组分信号转导系统cgtRS5的功能及调控机制

由ncgl2572-ncgl2573基因簇编码的双组分系统（双组分系统）CgtRS5在谷氨酸棒状杆菌的环境适应中起关键作用。野生型菌株和ΔcgtRS5突变株的转录组学比较分析显示，突变株中有3个基因上调，107个基因下调。其中，涉及铁代谢（ncgl1959）、芳香族化合物降解（ncgl2320）和耐药（cssR）的基因显著下调。表型分析表明ΔcgtRS5突变体在添加了二价铁（Fe 2 +）或烷基化剂碘乙酰胺（IAM）的培养基中表现出生长受损，但在苯甲酸盐、间苯二酚或抗生素胁迫下没有表现出显著差异。这些结果表明，CgtRS5特异性调控谷氨酸丙氨酸对铁和IAM的应激反应，为研究tcs介导的谷氨酸丙氨酸调控机制提供了新的思路。

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来源期刊

Journal of Basic Microbiology 生物-微生物学

CiteScore

6.10

自引率

0.00%

发文量

134

审稿时长

1.8 months

期刊介绍： The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions. Papers published deal with: microbial interactions (pathogenic, mutualistic, environmental), ecology, physiology, genetics and cell biology/development, new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications) novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).