Physiological and Molecular Responses of Anabaena laxa to Rac-Metalaxyl Fungicide: Exploring Metabolic and Oxidative Changes.

IF 3.5 4区 生物学 Q2 MICROBIOLOGY
Wael A Fathy, Emad A Alsherif, Shereen M Korany, Abeer S Aloufi, Samy Selim, Amira M Shaban
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引用次数: 0

Abstract

Rac-metalaxyl is a widely used fungicide for managing plant diseases; however, its environmental persistence and potential toxicity to aquatic microorganisms raise significant ecological concerns. Despite its widespread application, there is limited understanding of how cyanobacteria, which play vital ecological roles in aquatic systems, respond to such chemical stressors. This study addresses this gap by investigating the physiological and metabolic responses of the cyanobacterium Anabaena laxa to rac-metalaxyl exposure. Cultures were treated with 100 mg/L and 200 mg/L concentrations of the fungicide. Results showed increased intracellular accumulation of rac-metalaxyl at 200 mg/L, leading to significant reductions in cell growth, photosynthetic pigments, and the activities of key enzymes such as phosphoenolpyruvate carboxylase and ribulose-1,5-bisphosphate carboxylase/oxygenase. Further elevated lipid peroxidation levels indicated oxidative damage. Consequently, rac-metalaxyl triggered substantial metabolic shifts, total soluble sugars increased by 7.66% at 100 mg/L and 67.48% at 200 mg/L; malic acid rose to 76.23% at 200 mg/L; amino acids increased by 27.14% at 100 mg/L and 48.8% at 200 mg/L; total fatty acids rose by 10.11% at 100 mg/L and 35.06% at 200 mg/L. These findings suggest that Anabaena laxa exhibits coordinated oxidative and metabolic responses to mitigate rac-metalaxyl toxicity, highlighting its potential resilience and role in the bioremediation of contaminated aquatic environments.

疏水水藻对rac -甲氨酯杀菌剂的生理和分子反应:探讨代谢和氧化变化。
rac -甲螨灵是一种广泛应用于植物病害防治的杀菌剂;然而,它的环境持久性和对水生微生物的潜在毒性引起了重大的生态问题。尽管它的广泛应用,有有限的了解蓝藻,发挥重要的生态作用,在水生系统,如何应对这些化学应激源。本研究解决了这一差距,通过调查蓝藻Anabaena laxa的生理和代谢反应的rac-甲axyl暴露。用浓度分别为100 mg/L和200 mg/L的杀菌剂处理培养物。结果表明,当浓度为200 mg/L时,胞内乙酰甲axyl积累增加,导致细胞生长、光合色素和关键酶如磷酸烯醇丙酮酸羧化酶和核酮糖-1,5-二磷酸羧化酶/加氧酶活性显著降低。进一步升高的脂质过氧化水平表明氧化损伤。因此,丙甲axyl引发了大量的代谢变化,在100 mg/L和200 mg/L处理下,总可溶性糖增加了7.66%和67.48%;苹果酸在200 mg/L时上升到76.23%;氨基酸含量在100 mg/L和200 mg/L处理下分别增加了27.14%和48.8%;在100 mg/L和200 mg/L处理下,总脂肪酸含量分别上升10.11%和35.06%。这些发现表明,疏水水藻表现出协调的氧化和代谢反应,以减轻rac-metalaxyl的毒性,突出了其潜在的恢复能力和在污染水生环境的生物修复中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of Basic Microbiology
Journal of Basic Microbiology 生物-微生物学
CiteScore
6.10
自引率
0.00%
发文量
134
审稿时长
1.8 months
期刊介绍: The Journal of Basic Microbiology (JBM) publishes primary research papers on both procaryotic and eucaryotic microorganisms, including bacteria, archaea, fungi, algae, protozoans, phages, viruses, viroids and prions. Papers published deal with: microbial interactions (pathogenic, mutualistic, environmental), ecology, physiology, genetics and cell biology/development, new methodologies, i.e., new imaging technologies (e.g. video-fluorescence microscopy, modern TEM applications) novel molecular biology methods (e.g. PCR-based gene targeting or cassettes for cloning of GFP constructs).
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