Journal of Applied Phycology最新文献

{"title":"Growth and fatty acid profile of Nannochloropsis oceanica cultivated on nano-filtered whey permeate","authors":"Hossein Kiani, Qinge Ma, Mengsong Xiao, Yuchen Li, Felix Joel Brooke, Shane Mulcahy, Svitlana Miros, Ronald Halim","doi":"10.1007/s10811-024-03287-x","DOIUrl":"https://doi.org/10.1007/s10811-024-03287-x","url":null,"abstract":"<p>Nano-filtered whey permeate (WP), a major by-product of dairy industry, is produced by membrane filtration of whey. The oleaginous microalga <i>Nannochloropsis oceanica</i> was successfully cultivated on WP without salinity and nutrient amendments. Growth, cell characteristics, and fatty acid profile of the cultures were analyzed using microscopy, flow cytometry, and GC analysis. WP was nitrogen limited, comprising primarily protein as a nitrogen source and only small amounts of free inorganic nitrogen (in the form of nitrate). <i>Nannochloropsis oceanica</i> (and associated bacteria) efficiently removed nitrate (100%), protein (87%), and phosphate (74%) from the whey permeate. Microscopic and flow cytometric analysis revealed diverse size distributions in whey permeate cultures, with significant cell aggregation attributed to low-salinity acclimatization and nitrogen limitation. Autofluorescence analysis revealed reduced photosynthetic activity in whey permeate-grown cells, possibly as a consequence of heightened mixotrophic activities on carbon source in the medium. Low nitrogen availability in whey permeate resulted in biomass with a fatty acid profile enriched in saturated fatty acids. Despite this, a considerable level of the omega-3 polyunsaturated fatty acid (in the form of eicosapentanoic acid or EPA) was detected at ca. 16% of total fatty acids. Whey permeate proved beneficial for the growth of <i>N. oceanica</i> and yielded high concentrations of eicosapentaenoic acid in the extracted lipids for potential applications in the feed/food industries.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"44 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141547021","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Utilization of Sargassum crassifolium seaweed powder as a functional ingredient in wheat noodles","authors":"Dayani Imansa Egodavitharana, Sonali Manikkrama, Bambaranda Vidana Arachchilage Suneetha Manori Bambaranda, Deshani Chirajeevi Mudannayake","doi":"10.1007/s10811-024-03290-2","DOIUrl":"https://doi.org/10.1007/s10811-024-03290-2","url":null,"abstract":"<p>Edible brown seaweeds of the genus <i>Sargassum</i> have the potential to be utilized as nutrient-rich food components yielding beneficial health effects upon consumption. This study aimed to characterise <i>Sargassum crassifolium</i> for its phytochemical content and antioxidant capacity followed by its utilization as a dry powder in wheat noodles as a flour replacement. <i>Sargassum crassifolium</i> dehydrated powder contained considerable amounts of total phenols (1.13±0.05 mg GAE g^-1 DW), flavonoids (21.07±0.79 mg RE g^-1 DW), fucoidan (2.9±0.48 mg g^-1 DW), fucoxanthin (0.963±0.00 µg g^-1 DW), carotenoids (1.88±0.002 µg g^-1 DW) and tannins (1.88±0.002 mg g^-1 DW), while displaying significant DPPH (2,2-diphenyl-picrylhydrazyl hydrate) radical scavenging activity (23.20±1.73%). The use of 2.5% <i>S. crassifolium</i> as a flour replacement enhanced the sensory properties, nutrient composition and phytochemical content of the noodles. The DPPH radical scavenging activity of 2.5% <i>S. crassifolium</i> noodles (19.88±1.56%) in the cooked form was significantly higher than that of the control noodles containing no seaweed (13.29±2.02%). The results of this study revealed that <i>S. crassifolium</i> can be successfully incorporated in noodles to yield acceptable products that have improved nutrient content and potential functionality.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"1 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141523948","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unlocking the potential of Euglena gracilis cultivated in piggery wastewater: biomass production, nutrient removal, and biostimulant potential in lettuce and tomato plants","authors":"Valéria Louzada Leal Butzke, Alice Ferreira, Diego de Oliveira Corrêa, Júnior Mendes Furlan, Luisa Gouveia, Rosana de Cássia de Souza Schneider, Valeriano Antonio Corbellini","doi":"10.1007/s10811-024-03286-y","DOIUrl":"https://doi.org/10.1007/s10811-024-03286-y","url":null,"abstract":"<p>Microalgae are increasingly recognized as a valuable resource for bolstering sustainability in agriculture. However, current research and patents primarily focus on <i>Chlorella</i> spp., <i>Scenedesmus</i> spp., and <i>Spirulina</i> spp., thus leaving the vast diversity of microalgae relatively unexplored for agricultural applications. <i>Euglena gracilis</i> (Euglenophyta) is a microalga renowned for its resilience to diverse environmental stressors and capability to produce a variety of bioactive metabolites. This study investigated the potential of cultivating <i>E. gracilis</i> in piggery wastewater for nutrient recycling and as a source of beneficial biomolecules, particularly for biostimulant use. Utilizing raw wastewater diluted to 25% (P25) and pre-treated wastewater with photo-Fenton (PF), the research found that <i>E. gracilis</i> exhibited elevated cell density, biomass concentration, and overall cell health in both wastewaters compared to a synthetic medium (BG11-NPK). This was due to its efficient removal of nutrients, especially ammoniacal-nitrogen and phosphate, resulting in a biomass rich in polyunsaturated fatty acids, amino acids, and paramylon content. The whole-cell biomass significantly enhanced the germination index of lettuce and tomato seeds compared to the water control. Additionally, it promoted cell expansion and root formation in cucumber cotyledons, exhibiting similarities to phytohormones such as gibberellin, cytokinin, and auxin. Furthermore, it is suggested that <i>E. gracilis</i> biomass contains molecules related to resistance to environmental stresses, particularly in tomatoes, given the enhancement in the seedling vigor index. <i>E. gracilis</i> exhibited remarkable adaptability to piggery wastewater, recycling nutrients and yielding biomass rich in bioactive molecules with potential as plant biostimulants. These findings significantly contribute to understanding <i>E. gracilis</i>'s potential applications in agriculture and developing a circular bioeconomy.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"56 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-06-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141523949","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-assisted extraction of alginate from Ecklonia maxima with and without the addition of alkaline cellulase – factorial and kinetic analysis","authors":"Dylan van Sittert, Robert Lufu, Zwonaka Mapholi, Neill Jurgens Goosen","doi":"10.1007/s10811-024-03276-0","DOIUrl":"https://doi.org/10.1007/s10811-024-03276-0","url":null,"abstract":"<p>Ultrasound-assisted extraction and enzyme-assisted extraction are two methods that can be employed to recover valuable compounds from seaweeds at milder operating conditions than conventional chemical extraction methods. The aim of the study was to determine whether pH, enzyme addition and extraction temperature significantly impacted extraction of alginate from <i>Ecklonia maxima</i> in the presence of ultrasonication and to model extraction kinetics. Firstly, a 2³ factorial experimental design was employed at constant sonication rate, with pH (8 and 10), temperature (50 and 60 °C), and enzyme-to-substrate ratio (E:S 0 and 1 %) as the independent variables. Secondly, extraction kinetics of alginate were investigated at different extraction conditions, through fitting of the Peleg and Power models. Highest extraction for alginate was achieved at pH 10, 60 °C, E:S = 0 % within the first 60 min of extraction. The addition of enzyme improved solubilised dry matter content, but it had a less clear impact on the alginate yield. Both the Peleg (R² = 0.893 – 0.997) and Power function (R² = 0.861 – 0.987) models provided good fit to the experimental data and can used to describe extraction kinetics of alginate during combined ultrasound-enzymatic assisted extraction.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"77 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141193040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Ecklonia cava subspp. kurome and stolonifera ethanolic/aqueous extracts on caecal microbiota in mice fed a high-sucrose and low-dietary fibre diet","authors":"Sae Fujita, Kazuya Koga, Ayaka Nakamura, Hajime Takahashi, Yumeng Xia, Takashi Kuda","doi":"10.1007/s10811-024-03278-y","DOIUrl":"https://doi.org/10.1007/s10811-024-03278-y","url":null,"abstract":"<p>The traditional and local edible brown algae <i>Ecklonia cava</i> subspp. <i>kurome</i> (EK) and <i>stolonifera</i> (ES) are rich in minerals, phlorotannins (brown algal polyphenols), and water-soluble dietary fibres (alginate, laminaran, and fucoidans). These dried powders increase not only alginate- and laminaran-degrading <i>Bacteroides acidifaciens-</i> and <i>B. intestinalis-</i>like bacteria but also <i>Faecalibaculum</i>. To elucidate the effect of EK and ES ingredients, other than dietary fibres, on gut microbiota, 100 mL of ethanolic/aqueous extract solutions (EK-S, ES-S; final solvent was distilled water) were prepared from 100 g of dried samples. EK-S and ES-S contained total phenolic contents of 160 and 120 μmol phloroglucinol equivalent mL^-1, respectively. A diet containing 5% (v/w) EK-S or ES-S was fed to ICR mice for 14 days. Amplicon sequencing of the 16S rDNA (V4) gene revealed that EK-S and ES-S increased <i>Faecalibaculum</i> abundance 2-fold and more, and this was the predominant genus (37–38%) in mice. However, the abundances of <i>B. acidifaciens-</i> and <i>B. intestinalis-</i>like bacteria did not increase. <i>Akkermansia muciniphila</i>-like bacteria were higher in EK-S-fed mice. <i>Faecalibaculum</i> and <i>Akkermansia</i> are regarded as desirable gut commensals correlated with anti-inflammatory and immunomodulatory effects on the host’s health. These results suggest that phlorotannins have beneficial functional effects on indigenous gut bacteria responsible for host health.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"102 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141193037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"FlaskLED: an additive manufacturing approach for low-cost illuminated culture flask bioreactors","authors":"Omri Landschaft, Asher Wishkerman","doi":"10.1007/s10811-024-03260-8","DOIUrl":"https://doi.org/10.1007/s10811-024-03260-8","url":null,"abstract":"<p>This methodology paper introduces an illuminated culture flask-based 3D-printed bioreactor system (FlaskLED) based on Arduino, Grove ecosystem and LED sticks. The Arduino microcontroller allows extensibility and future upgrades via programming and other modifications. We provide the 3D printer files, video presentation of assembly instructions and a detailed description of the INO code and wiring scheme. The INO code presented can be used to produce different light colors and patterns of varying intensity and durations (intermittent/flashing/pulsing light). The system was validated by examining the toxicity of Au³⁺ ions on <i>Phaeodactylum tricornutum</i> growth. The capabilities of the system offer unique applications for laboratory and industrial research. Our aim is to provide a low-cost and open-source tool to promote and improve cultivation of microalgae and other photosynthetic organisms under different laboratory and experimental setups.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"47 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141193237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cultivation of edible Caulerpa species in Malaysia: Current status and future prospects for sustainable aquaculture","authors":"Mohd Fakhrulddin Ismail, Farah Izana Abdullah, Illisriyani Ismail, Murni Marlina Abd Karim, Shiamala Devi Ramaiya, Mohammad Amil Zulhilmi Benjamin, Mohd Azrie Awang, Muta Harah Zakaria","doi":"10.1007/s10811-024-03271-5","DOIUrl":"https://doi.org/10.1007/s10811-024-03271-5","url":null,"abstract":"<p>The deficiency in the worldwide protein provision from marine capture fisheries has led the Malaysian government to reevaluate its aquaculture approach, prioritising three commodities: seaweed, fish, and marine shrimp. However, comprehensive documentation of the performance of the Malaysian aquaculture sector, particularly in seaweed production, is lacking. <i>Caulerpa </i>(Chlorophyta), a seaweed genus abundantly available in Malaysia, holds the potential to emerge as a primary alternative food source in the future. This paper offers an overview of <i>Caulerpa</i> aquaculture, specifically focusing on <i>C. lentillifera</i> and <i>C. racemosa</i>, covering aspects such as taxonomy, phytomorphology, geographical distribution and habitat, cultivation system, chemical composition, pharmacological properties, and future prospects for sustainable aquaculture. Although the cultivation system has been implemented on a small scale in various districts in Malaysia, it is anticipated to escalate production and productivity due to the substantial demand for <i>Caulerpa</i> spp. both domestically and globally. The cultivation of <i>Caulerpa</i> spp. in Malaysia, in alignment with the National Agrofood Policy 2021–2030 (NAP 2.0), signals a trajectory toward enhancing the nation's food security in aquaculture and meeting the economic requirements for seaweed production.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"20 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141193152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A methylene blue assay for the quantification of fucoidan – A specific application for seaweed extracts","authors":"Gina Lauren Wilkinson, Zwonaka Mapholi, Neill Jurgens Goosen","doi":"10.1007/s10811-024-03257-3","DOIUrl":"https://doi.org/10.1007/s10811-024-03257-3","url":null,"abstract":"<p>Fucoidan is a fucose-rich sulfated polysaccharide found mainly in brown seaweeds. Fucoidan has recently gained much attention due to its bioactive and medicinal properties, hence its high potential to be utilized in the pharmaceutical industry. Therefore, an accurate, reliable, and simple method for the specific determination of fucoidan would be a useful tool when looking to recover this valuable polysaccharide. This research focused on developing an assay utilizing the thiazine dye, methylene blue, for the quantification of fucoidan, with a specific application for fucoidan found in algal extracts. Fucoidan could be specifically determined at pH 1, to maximum concentrations of 4 g L^-1 in solution without the interference of alginate and other seaweed extract contaminants. At pH 7 and 11, quantification of fucoidan led to interference from alginate and gallic acid. A conversion factor of 1.8 allowed the quantification of fucoidan from <i>Ecklonia maxima</i> extracts using a commercial standard from <i>Fucus vesiculosis.</i> An <i>Ecklonia maxima</i> extract was found to contain 5.47 ± 0.061 g L^-1 of fucoidan. Measuring the fucoidan and L-fucose concentration in crude seaweed extract through the methylene blue assay and modified Dische and Shettles J Biol Chem 175:595–603, (1948) method, respectively, confirmed the accuracy and specificity of the method. The limit of quantification of the methylene blue assay at pH 1 was 0.62 g L^-1 of fucoidan.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"131 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141061215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advances of high-throughput sequencing for unraveling biotechnological potential of microalgal-bacterial communities","authors":"Petr A. Zaytsev, Vladimir A. Rodin, Anna A. Zaytseva, Maria I. Zvereva, Alexei E. Solovchenko","doi":"10.1007/s10811-024-03267-1","DOIUrl":"https://doi.org/10.1007/s10811-024-03267-1","url":null,"abstract":"<p>Although established biotechnological applications of microalgae e.g., the production of high-value metabolites is based on axenic cultures, exploitation of the mutualistic consortia of microalgae and bacteria quickly comes to foreground, especially in bioremediation and wastewater treatment. This trend shifts the focus from genomic research of certain microalgal species to metagenomic studies of interactions between microalgae and bacteria in natural communities and in artificial consortia. Dissection of the genetic determinants of the robustness and productivity of the consortia become a hot research direction, too. Admirable contribution to this topic had been made by high-throughput sequencing (HTS), while recent breakthrough in this field was entailed by the advent and rapid development of the 3rd generation nanopore sequencing which becomes increasingly accurate while providing unprecedented sequencing performance. Recent progress of the Oxford Nanopore Technologies (ONT) enabled both classical metagenomic analysis of microalgal-bacterial communities based on whole metagenome sequencing as well as taxonomic and genetic profiling based on the amplicon sequencing. The parallel emergence of novel bioinformatic algorithms for processing the metagenomic datasets opened new opportunities for the analysis of structure and physiology of microalgal-bacterial communities. From the practical perspective, the new HTS techniques became a time- and labor-savers in discovery of new microalgae with a high potential for the accumulation of valuable metabolites, biodegradation of hazardous micropollutants, and biosequestration of nutrients from waste streams. Search for prokaryotic species boosting the biotechnological potential of eukaryotic microalgae via mutualistic interactions with them is another important goal. The insights from the both short-read and long-read metagenomics will form a solid foundation for the rational design of microalgal-bacterial consortia for biotechnology. In this review, we briefly outline the benefits of the long-read sequencing for structural and functional investigation of algal-bacterial consortia and summarize recent reports on using this approach for achieving the biotechnology-related goals.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"28 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141060866","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Concise review of the kelp genus Eisenia Areschoug","authors":"Jose Avila-Peltroche, Maria-Lourdes Avalos, Jasmin Scholl Chirinos","doi":"10.1007/s10811-024-03264-4","DOIUrl":"https://doi.org/10.1007/s10811-024-03264-4","url":null,"abstract":"<p><i>Eisenia</i> (order Laminariales) is a genus of warm-tolerant kelps found in the Northern and Southern Hemispheres of the East Pacific, as well as in the North West Pacific, where species form beds in rocky bottoms along the sea coast from the intertidal and subtidal zones. There are seven recognized species in the genus, including the endemic and vulnerable <i>E. galapagensis</i>. Despite some remarkable morphological variations, <i>Eisenia</i> species are characterized by two twisted stipe-like parts arising from the main stipe. Genetic data have recently contributed to the understanding of <i>Eisenia</i> systematics; however, additional studies on <i>E. desmarestioides</i>, <i>E. cokeri</i>, and <i>E. gracilis</i> are needed. Aside from economic benefits, <i>Eisenia</i> farming has various other essential benefits, such as resource sustainability and ecosystem benefits, which are especially important in climate change scenarios. Spore-based cultivation has been reported only for <i>E. arborea, E. bicyclis,</i> and <i>E. nipponica</i> (formerly known as <i>E. arborea sensu</i> Arasaki). This kelp is widely consumed in Korea and Japan and utilized as a raw material for alginate production and feed for abalone cultures. Because of its nutritional composition and mineral concentration, <i>Eisenia</i> is also a potential food source outside Asia. Moreover, various pharmacological applications of <i>Eisenia</i> have been reported, particularly those related to antioxidant, antidiabetic, and anti-inflammatory properties. Phlorotannins (brown algal polyphenols) derived from <i>E. bicyclis</i> are linked to these bioactivities. In this work, we present a comprehensive synthesis of the current state of knowledge on <i>Eisenia</i>, offering insights into its taxonomy, distribution, ecology, cultivation, and various applications.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"60 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141061305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}