Journal of Applied Phycology最新文献

{"title":"Assessing the potential of C-phycocyanin as a natural colorant for non-alcoholic carbonated beverages","authors":"Tonmoy Ghosh, Ismail Rawat, Kiran Bala, Sandhya Mishra, Faizal Bux","doi":"10.1007/s10811-024-03235-9","DOIUrl":"https://doi.org/10.1007/s10811-024-03235-9","url":null,"abstract":"<p>The multibillion-dollar carbonated beverage industry is currently facing questions from health-conscious consumers over negative health effects of such beverages. Decreasing consumption trends have forced companies to look for healthier choices for their products. C-phycocyanin CPC, a bright blue cyanobacterial pigment with anti-oxidant and other health benefits has been proposed as a candidate in edible drinks. We found that CPC is stable in a wide pH and temperature regime. Reaction kinetics for 12 weeks at 4 °C in non-alcoholic carbonated beverages (B1-B4) showed that B3 (sweetened, ~30 % degradation) best preserved CPC integrity while B1 (non-sweetened, ~87 % degradation) was ineffective. Other beverages (sweetened) could preserve ~ 49 % CPC integrity. Behnajady-Modirshahla-Ghanbary and first order kinetic models explained CPC degradation with and without preservative (sucrose), respectively. The ’consume-by’ times suggest possible refrigeration from ~ 13 hours to 27 days for various CPC-containing beverages. Results suggest CPC could be filter-sterilized and added to non-alcoholic beverages before being packaged in cans or tetra packs to avoid light exposure.</p><h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>\u0000","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"8 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140559922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fast screening method to identify salinity tolerant strains of foliose Ulva species. Low salinity leads to increased organic matter of the biomass","authors":"Clara Simon, Antoine Fort, Diane Jouanneau, Marcus McHale, Ronan Sulpice","doi":"10.1007/s10811-024-03222-0","DOIUrl":"https://doi.org/10.1007/s10811-024-03222-0","url":null,"abstract":"<p>Sea lettuce (<i>Ulva</i>) is recognised for its potential in food, pharmaceutical, nutraceutical, biorefinery and bioremediation industries and is increasingly being cultivated. The requirements of those industries vary widely in terms of biomass composition. <i>Ulva</i> biomass composition and growth is known to be directly influenced by environmental factors, e.g., temperature, light, salinity, nutrient availability as well as by genetic factors and likely by microbiome composition. In order to select for the highest yielding strains in a given environment, we tested the suitability of common-garden experiments, i.e., the co-cultivation of different strains grown under shared conditions. Fifteen strains from six different foliose <i>Ulva</i> species were grown together under two different salinities, 35 ppt and 15 ppt. After 32 days, only <i>U. australis</i> strains remained at both salinities. If selection at low salinity was mostly based on survival, the selection process at seawater salinity was driven by competition, largely based on growth performance. Growth rates after a month were very similar at both salinities, suggesting the <i>U. australis</i> strains cope equally well in either condition. However, the composition of the biomass produced in both environments varied, with the content of all organic compounds being higher at low salinity, and the ash content being reduced in average by 66%. To summarize, this study provides an established bulk-selection protocol for efficiently screening large numbers of locally-sourced strains and highlights the potential of low salinity treatments for increased organic matter content, particularly in carbohydrates.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"91 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140599441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The commercial potential of Aphanizomenon flos-aquae, a nitrogen-fixing edible cyanobacterium","authors":"Gabriel D. Scoglio, Harry O. Jackson, Saul Purton","doi":"10.1007/s10811-024-03214-0","DOIUrl":"https://doi.org/10.1007/s10811-024-03214-0","url":null,"abstract":"<p><i>Aphanizomenon flos-aquae (AFA)</i> is a Gram-negative nitrogen-fixing freshwater filamentous cyanobacterium with a rich nutritional profile that is approved for human consumption by both the Food and Drug Administration and the European Food and Safety Authorities. It has a high protein content (60-70%) and contains numerous vitamins, minerals and trace elements together with several high-value compounds with nutraceutical properties, such as C-phycocyanin and β-phenylethylamine. 500-1000 t of <i>AFA</i> dry biomass are currently wild harvested from natural blooms that occur seasonally in Klamath Lake, Oregon, USA, and distributed as a nutritional supplement worldwide. The requirements and unreliability of wild harvesting, owing to the dependence of <i>AFA</i> growth on environmental conditions and potential contamination by microcystin toxins, threaten the availability of biomass supply and restricts commercial expansion. In this review we propose <i>AFA</i> cultivation in open ponds or closed photobioreactors to obtain a reliable production of unialgal biomass to resolve the supply issue and enhance <i>AFA</i> as a feedstock for specific high-value by-products. Furthermore, we explore potential strategies for maximising overall yield and seasonal robustness by using a synergistic combination of cultivation and wild harvesting. Controlled <i>AFA</i> cultivation would also facilitate the use of genetic manipulation to generate bespoke strains with improved commercial applications, such as increasing the cyanobacterium’s nitrogen-fixation rate to enhance its value as a biofertiliser. Ultimately, realising the untapped biotechnological potential of <i>AFA</i> requires a better understanding of its fundamental biology, robust methodologies for laboratory and large-scale cultivation, and the development of <i>AFA</i>-specific genetic engineering techniques.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"15 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140599429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of microalgae feed supplementation on growth performance and feeding efficiency of tilapia fry","authors":"","doi":"10.1007/s10811-024-03232-y","DOIUrl":"https://doi.org/10.1007/s10811-024-03232-y","url":null,"abstract":"<h3>Abstract</h3> <p>Search for novel best-performing starter feed ingredients is one of the main challenges of modern aquaculture industry. The goal of this article is to evaluate the effect of microalgae supplementation enriched with omega-3 polyunsaturated eicosapentaenoic acid and the powerful antioxidant fucoxanthin on growth performance of Red tilapia fry (<em>Oreochromis mossambicus</em> × <em>Oreochromis niloticus</em>). We formulated three experimental diets, using two strains of microalgae from our culture collection – <em>Vischeria magna</em> SBV-108 (Eustigmatophyceae), rich in eicosapentaenoic acid and <em>Mallomonas furtiva</em> SBV-13 (Chrysophyceae), rich in fucoxanthin – and their combination. <em>Vischeria magna</em> SBV-108 is a new strain which biochemical properties and growth characteristics have not been previously studied. Coppens Supreme-15 was used as a control diet. The best growth performance was recorded in eicosapentainoic acid enriched Diet 1 (10% w/w <em>V. magna</em>), that significantly (P&lt;0.05) overperformed control feed on absolute growth, average growth, specific growth rate, feed conversion rate, protein efficiency ratio, protein productive value and energy productive value. Diet 3 (5% w/w <em>V. magna</em> and 5% w/w <em>M. furtiva</em>) and Diet 2 (10% w/w <em>M. furtiva</em>) were not significantly different from control at most parameters. Generally, supplementation of feed with microalgae rich in omega-3 eicosapentaenoic acid significantly improved Red tilapia fry growth parameters and overall feed performance, while supplementation of feed with microalgae rich in antioxidant fucoxanthin was not beneficial compared to the control.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"300 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140599440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural biocontrol of a Porphyra sp. pest on farmed Gracilaria chilensis by a pythiosis outbreak","authors":"Liliana Muñoz, David J. Patiño, Pedro Murúa","doi":"10.1007/s10811-024-03228-8","DOIUrl":"https://doi.org/10.1007/s10811-024-03228-8","url":null,"abstract":"<p><i>Gracilaria chilensis</i> (a.k.a. pelillo) is the most produced seaweed in Chile and Latin America, yet its cultivation has historically faced lots of pest-associated constrains that threat its profitability and sustainability. Pests show temporal cycles of recruitment, growth and death/senescence, variation normally linked with sharp changes in environmental factors occurring in estuarine areas whereby <i>Gracilaria</i> is cultivated. Here we report the appearance of a bladed Bangiales species epiphytic on long-line farmed <i>Gracilaria</i> and identified as <i>Porphyra</i>. This species recruits to cover up to 50–72% of <i>G. chilensis</i> early in a suspended set-up in spring, until a filamentous fungal-like organism colonizes <i>Porphyra</i> blades, infecting a wide proportion of its tissue. After this outbreak, <i>Porphyra</i> recruits collapse, disappearing in few weeks from farmed <i>Gracilaria</i>. Observations of diseased individuals, and subsequent isolation and marker-assisted taxonomy of the pathogen, provide evidence for the identification of this organism as <i>Pythium porphyrae</i>, the aetiological agent for the red rot disease in commercial nori/gim in Asia. This is the first reported case for <i>P. porphyrae</i> in Chile and the Southeastern Pacific as well as for a disease-driven natural biocontrol of a <i>Gracilaria</i> pest alga, suggesting an unknown -yet considerable- cryptic biodiversity acting as natural regulators of natural pests during a <i>Gracilaria</i> cultivation cycle.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"96 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140599577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The 24th International Seaweed Symposium - ‘Seaweeds in a changing world’","authors":"Daniel Robledo","doi":"10.1007/s10811-024-03217-x","DOIUrl":"https://doi.org/10.1007/s10811-024-03217-x","url":null,"abstract":"<p>The 24th edition of the International Seaweed Symposium (ISS), an academic-industry event that dates back to 1952, was held for the first time in Oceania. The local organizing committee chaired by Professor Catriona Hurd, Institute for Marine and Antarctic Studies, University of Tasmania, and Professor Michael A. Borowitzka, Murdoch University, Western Australia worked tirelessly to curate a program that balanced scientific rigor with engaging discussions, providing ample opportunities for networking and collaboration. “<i>Seaweeds in a changing world</i>” was the theme of this symposium, participants from 48 countries from all over the world attended this symposium from 19-24 February 2023. More than 576 on-site and 207 virtual participants registered for the Symposium who contributed to the success of this event. The International Seaweed Association (ISA) ensures the continued development of seaweed and its benefits and serves as a bridge between research academia and industry. The ISA is ready to celebrate the Silver Jubilee of the ISS and is also proud to announce that the 25th International Seaweed Symposium (ISS) will be held in Victoria, British Columbia, Canada, 4-9 May, 2025 with the Local support of Ocean Networks Canada, Cascadia Seaweed, and the Pacific Seaweed Industry Association (https://iss25.com).</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"38 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140599435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can Ascophyllum nodosum extract application before or at drought stress trigger different metabolic adaptation responses in soybean plants?","authors":"","doi":"10.1007/s10811-024-03231-z","DOIUrl":"https://doi.org/10.1007/s10811-024-03231-z","url":null,"abstract":"<h3>Abstract</h3> <p>Soybean is essential for food security worldwide and water restriction is a rising challenge for its production. In this scenario the use of natural sources such as <em>Ascophyllum nodosum</em> extracts (ANE) which contribute to the better adaptation of plants to environmental stresses, gains relevance. Nonetheless, understanding the metabolic effect of ANE according to the time of application could be better explored, such as, if the application before or during the stress occurrence can trigger different metabolic responses. Thus, this work aimed to identify the physiological and biochemical effects of foliar application of ANE at different times, in soybean plants subjected to water restriction. The experiment was carried out in pots with plants at the vegetative stage, subjected to foliar sprays of ANE in the following treatments: (i) application of ANE at adequate humidity with 80% of the substrate water holding capacity (WHC); (ii) application of ANE three days before the humidity reached 50% of the substrate WHC; (iii) application of ANE on the day that WHC reached 50% (established as drought stress condition); (iv) positive control without application of ANE and WHC 50%; (v) absolute control, without application of ANE and without water restriction. The results allow to point out that ANE application three days before drought stress improved nitrate reductase enzyme activity, chlorophyll and proline content, decrease lipid peroxidation and presented more effective improvement of peroxidase enzyme activity; while application at stress condition resulting in more effective reduction of stomata resistance, long term expressive proline content increment, more effective improvement of superoxide dismutase and catalase enzymes activity and reduce chlorophyll degradation. This works results also could help to answer some field practice questions, such as; it is worth applying ANE when plants are already under drought stress.</p> <span> <h3>Graphical Abstract</h3> <p><em>Ascophyllum nodosum</em> extracts (ANE) applied three days before drought stress (A) improved nitrate reductase enzyme activity (NR), proline (PR) and chlorophyll content (Chl), decreased lipid peroxidation (LP) and presented more effective improvement of peroxidase enzyme activity (POD). Application at stress condition (B) resulting in more effective reduction of stomata resistance (SR) and chlorophyll (Chl) degradation, long term proline content increment (PR), more effective improvement of superoxide dismutase (SOD) and catalase (CAT) enzymes activity.</p> <p> <span> <span> <img alt=\"\" src=\"https://static-content.springer.com/image/MediaObjects/10811_2024_3231_Figa_HTML.png\"/> </span> </span></p> </span>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"30 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140599580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cyanobacterial biofertilizer inoculation has a distinctive effect on the key genes of carbon and nitrogen cycling in paddy rice","authors":"Babanpreet Kour, Preeti Sharma, S. Ramya, Sandeep Gawdiya, K Sudheer, Balasubramanian Ramakrishnan","doi":"10.1007/s10811-024-03230-0","DOIUrl":"https://doi.org/10.1007/s10811-024-03230-0","url":null,"abstract":"<p>Cyanobacterial biofertilizers provide soil fertility and productivity gains at varying levels in paddy rice cultivation. The colonization and influences of introduced strains in different soil types with characteristic compositions of native cyanobacteria remain largely unknown. In this work, seven paddy rice soils with the composition of indigenous cyanobacteria described by amplicon sequencing analysis were inoculated with the cyanobacterial biofertilizer. The microbial abundance and the cyanophage concentrations were evaluated under light-dark or continuous dark cycles using quantitative polymerase chain reaction (qPCR) assays. The copies of cyanobacterial-16S rRNA gene markers varied from 5.65 × 10⁶ to 9.22 × 10⁷ g^-1 soil, and their abundance increased significantly in the inoculated soils. The cyanophage concentrations, quantified using the capsid assembly protein gene <i>g20</i> in the soils tested, ranged from 3.04 × 10⁸ to 9.24× 10⁸ g^-1 soil on 30 days after incubation. There were significant increases in the abundance of the <i>nifH</i> gene copies, about 1.54×10⁵ to 1.35×10⁶ g^-1, in the inoculated soils, albeit with soil type-specific responses. The gene markers of C and N cycling (i.e., <i>cbbL</i> and <i>nifH,</i> respectively), taxonomic markers (of archaea, bacteria, and cyanobacteria), and cyanophage-specific gene copies showed strong and positive correlation with the cyanobacterial biofertilizer inoculation. However, the genes related to nitrification (bacterial and archaeal <i>amoA</i>) and denitrification (<i>nirS, nirK, narG,</i> and <i>nosZ</i>) were clustered together in the uninoculated soils. The rice rhizospheres in three representative paddy soil types, using metatranscriptomics analysis, showed distinctive colonization by cyanobacteria, with several members yet to be described. These results indicate the potential for improving cyanobacterial biofertilizers for their contributions to plant growth and fertility gains in a soil-specific way.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"50 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140198901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of different methods for extraction of phycocyanin from the cyanobacterium Arthrospira maxima (Spirulina)","authors":"Jan Kuhnholz, Till Glockow, Verena Siebecke, Anh Thu Le, Long-Dinh Tran, Anja Noke","doi":"10.1007/s10811-024-03224-y","DOIUrl":"https://doi.org/10.1007/s10811-024-03224-y","url":null,"abstract":"<p>Phycocyanin is an interesting alternative to synthetic food colorants. Various methods to obtain phycocyanin from <i>Arthrospira</i> (Spirulina) biomass have been described in the literature, including ultrasonication, glass bead extraction and freeze-thawing. In this study, three optimized procedures were implemented to assess their efficacy in obtaining phycocyanin from <i>Arthrospira maxima</i> biomass, facilitating a comparative analysis of their effectiveness. After harvesting the biomass, extraction processes were conducted utilizing ultrasonication followed by flocculation with chitosan in various organic acid solutions, as well as glass bead extraction and freeze-thawing techniques, each followed by centrifugation. The obtained extracts were analyzed spectrophotometrically across the wavelength range of 280 to 800 nm. The freeze-thawing method yielded the highest C-PC contents at 17.03 ± 0.53%, followed closely by the ultrasonication method at 15.21 ± 0.41%. The highest purity of 2.02 ± 0.01 was attained through ultrasonication and subsequent flocculation with chitosan in acetic acid. Conversely, employing chitosan dissolved in citric or lactic acid for flocculation resulted in greenish extracts containing high amounts of chlorophyll.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"2 7 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140198894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of freezing and thawing on Alaria esculenta","authors":"Randi Sund, Turid Rustad, Arne Duinker, Dagbjørn Skipnes","doi":"10.1007/s10811-024-03226-w","DOIUrl":"https://doi.org/10.1007/s10811-024-03226-w","url":null,"abstract":"<p>Seaweeds must be stabilised shortly after harvesting to avoid rapid deterioration. To handle large amounts harvested during a short period, freezing and frozen storage until utilisation or further processing is one of the methods used industrially. The aim of this study was to assess the effects of different freezing and thawing procedures on <i>Alaria esculenta</i> by analysis of the chemical composition of the seaweed and the drip loss expelled during thawing. Thawing of industrially frozen <i>A. esculenta</i> resulted in a drip loss of 57% of wet weight. The drip loss had a dry matter content of 7% of wet weight, of which 71% was mineral content. Analysis showed that, of the dry matter excluding ash, alanine, aspartic acid, and mannitol were the main components lost to the drip loss. Experiments with a second batch of <i>A. esculenta</i> looking at quick and slow freezing and thawing showed that quick freezing resulted in a significantly lower drip loss than slow freezing; 20% compared to up to 42% of wet weight. Dry matter and mineral content of the drip loss of these samples were all around 6% of wet weight and 31% of dry weight. For some applications it might be of interest to reduce the concentration of potentially toxic elements such as iodine and heavy metals, but due to a high loss of other biomass this was not very effectively done by freezing and thawing. For preservation purposes, quick freezing is the best alternative to retain seaweed biomass.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"142 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-03-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140198756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}