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引用次数: 0
摘要
本方法论论文介绍了基于 Arduino、Grove 生态系统和 LED 灯棒的发光培养瓶 3D 打印生物反应器系统（FlaskLED）。Arduino 微控制器允许通过编程和其他修改进行扩展和未来升级。我们提供 3D 打印机文件、组装说明视频演示以及 INO 代码和布线方案的详细说明。所介绍的 INO 代码可用于产生不同颜色、不同强度和持续时间（间歇光/闪烁光/脉冲光）的光模式。该系统通过检测 Au3+ 离子对 Phaeodactylum tricornutum 生长的毒性进行了验证。该系统的功能为实验室和工业研究提供了独特的应用。我们的目标是提供一种低成本、开源的工具，以促进和改善不同实验室和实验装置下微藻类和其他光合生物的培养。
FlaskLED: an additive manufacturing approach for low-cost illuminated culture flask bioreactors
This methodology paper introduces an illuminated culture flask-based 3D-printed bioreactor system (FlaskLED) based on Arduino, Grove ecosystem and LED sticks. The Arduino microcontroller allows extensibility and future upgrades via programming and other modifications. We provide the 3D printer files, video presentation of assembly instructions and a detailed description of the INO code and wiring scheme. The INO code presented can be used to produce different light colors and patterns of varying intensity and durations (intermittent/flashing/pulsing light). The system was validated by examining the toxicity of Au3+ ions on Phaeodactylum tricornutum growth. The capabilities of the system offer unique applications for laboratory and industrial research. Our aim is to provide a low-cost and open-source tool to promote and improve cultivation of microalgae and other photosynthetic organisms under different laboratory and experimental setups.
期刊介绍：
The Journal of Applied Phycology publishes work on the rapidly expanding subject of the commercial use of algae.
The journal accepts submissions on fundamental research, development of techniques and practical applications in such areas as algal and cyanobacterial biotechnology and genetic engineering, tissues culture, culture collections, commercially useful micro-algae and their products, mariculture, algalization and soil fertility, pollution and fouling, monitoring, toxicity tests, toxic compounds, antibiotics and other biologically active compounds.
Each issue of the Journal of Applied Phycology also includes a short section for brief notes and general information on new products, patents and company news.
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