{"title":"FlaskLED: an additive manufacturing approach for low-cost illuminated culture flask bioreactors","authors":"Omri Landschaft, Asher Wishkerman","doi":"10.1007/s10811-024-03260-8","DOIUrl":null,"url":null,"abstract":"<p>This methodology paper introduces an illuminated culture flask-based 3D-printed bioreactor system (FlaskLED) based on Arduino, Grove ecosystem and LED sticks. The Arduino microcontroller allows extensibility and future upgrades via programming and other modifications. We provide the 3D printer files, video presentation of assembly instructions and a detailed description of the INO code and wiring scheme. The INO code presented can be used to produce different light colors and patterns of varying intensity and durations (intermittent/flashing/pulsing light). The system was validated by examining the toxicity of Au<sup>3+</sup> ions on <i>Phaeodactylum tricornutum</i> growth. The capabilities of the system offer unique applications for laboratory and industrial research. Our aim is to provide a low-cost and open-source tool to promote and improve cultivation of microalgae and other photosynthetic organisms under different laboratory and experimental setups.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"47 1","pages":""},"PeriodicalIF":2.8000,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Phycology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10811-024-03260-8","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
This methodology paper introduces an illuminated culture flask-based 3D-printed bioreactor system (FlaskLED) based on Arduino, Grove ecosystem and LED sticks. The Arduino microcontroller allows extensibility and future upgrades via programming and other modifications. We provide the 3D printer files, video presentation of assembly instructions and a detailed description of the INO code and wiring scheme. The INO code presented can be used to produce different light colors and patterns of varying intensity and durations (intermittent/flashing/pulsing light). The system was validated by examining the toxicity of Au3+ ions on Phaeodactylum tricornutum growth. The capabilities of the system offer unique applications for laboratory and industrial research. Our aim is to provide a low-cost and open-source tool to promote and improve cultivation of microalgae and other photosynthetic organisms under different laboratory and experimental setups.
期刊介绍：
The Journal of Applied Phycology publishes work on the rapidly expanding subject of the commercial use of algae.
The journal accepts submissions on fundamental research, development of techniques and practical applications in such areas as algal and cyanobacterial biotechnology and genetic engineering, tissues culture, culture collections, commercially useful micro-algae and their products, mariculture, algalization and soil fertility, pollution and fouling, monitoring, toxicity tests, toxic compounds, antibiotics and other biologically active compounds.
Each issue of the Journal of Applied Phycology also includes a short section for brief notes and general information on new products, patents and company news.
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