Journal of Applied Phycology最新文献

{"title":"Successful growth of coastal marine microalgae in wastewater from a salmon recirculating aquaculture system","authors":"Stian Borg-Stoveland, Vukasin Draganovic, Kristian Spilling, Tove M. Gabrielsen","doi":"10.1007/s10811-024-03310-1","DOIUrl":"https://doi.org/10.1007/s10811-024-03310-1","url":null,"abstract":"<p>As global demand for seafood increases, recirculating aquaculture systems (RAS) have gained prominence for sustainable fish rearing. The sustainability of RAS still requires improvement, particularly managing the fish waste. Here we investigated the growth and nutrient removal capabilities of three microalgal species (<i>Isocrysis galbana, Phaeodactylum tricornutum</i> and <i>Skeletonema marinoi</i>) in aquaculture wastewater (AWW) mixed at different concentrations with cultivation medium. All three microalgae showed growth in different concentrations of the AWW obtained from an Atlantic salmon RAS facility in Agder, Norway. The average growth rates for <i>I. galbana</i>, <i>S. marinoi</i> and <i>P. tricornutum</i> at 75% AWW concentration were 0.31± 0.00 day^-1, 0.34 ± 0.00 day^-1, 0.25 ± 0.02 day^-1, respectively. All three species effectively contributed to nitrate, ammonium and phosphate removal. When cultivated in 75% AWW, the microalgae achieved nearly complete removal of nitrite, nitrate, and phosphate, while approximately 90% of ammonium was also removed. Our results confirm the viability of AWW for microalgal cultivation on a laboratory scale suggesting this presents a sustainable route to further develop a circular bioeconomy in aquaculture.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"32 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141609816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Uses and applications of the red seaweed Kappaphycus alvarezii: a systematic review","authors":"Aline Nunes, Gadiel Zilto Azevedo, Felipe de Souza Dutra, Beatriz Rocha dos Santos, Alex Ricardo Schneider, Eva Regina Oliveira, Sidnei Moura, Fábio Vianello, Marcelo Maraschin, Giuseppina Pace Pereira Lima","doi":"10.1007/s10811-024-03270-6","DOIUrl":"https://doi.org/10.1007/s10811-024-03270-6","url":null,"abstract":"<p><i>Kappaphycus alvarezii</i> is the fifth most cultivated macroalga in the world, with many industrial applications. Understanding the advances in research on <i>K. alvarezii</i> is essential to further biotechnological applications. Using PRISMA criteria a study was carried out on the CAPES Journals Portal database, between 2017-2023, with “<i>Kappaphycus alvarezii</i>” as single descriptor. Results (<i>n</i> = 559) were obtained and after defining the criteria, 152 manuscripts were subdivided into the following areas: cosmetics (<i>n</i> = 5), animal nutrition (<i>n</i> = 12), human food (<i>n</i> = 20), health/medicine (<i>n</i> = 33), agriculture (<i>n</i> = 34), and general industry (<i>n</i> = 48). From the results, the use of <i>K. alvarezii</i> biomass in the formulation of cosmetics for skin treatment and as a curative can be highlighted. Broad benefits were reported to humans and animals in food and feed supplementation. Moreover, in view of its bioactivities, certain metabolites have the potential to be used for developing new drugs due to their antitumor, antidiabetic, anti-inflammatory and neuroprotective activities. In agriculture, using various processes to make an extract, increases of plant yield and quality have been observed, reducing biotic and abiotic stresses and as a resistance elicitor. <i>Kappaphycus alvarezii</i> biomass has been used for producing biofuel and nanomaterials developments, and for the production of biofilms, especially for the food packaging industry. Thus, <i>K. alvarezii</i> presents wide industrial applications, not only due to the use of carrageenan. Therefore, it deserves further innovations, as it represents a matrix rich in bioactive compounds and is environmentally friendly, possibly replacing harmful ingredients currently used by industry.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"20 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141585133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extraction and optimization of biomolecules recovery from Chlorella sp. NITT 02: Unleashing potential through ultrasound and triphasic system of extraction","authors":"Susaimanickam Anto, Manickam Premalatha, Thangavel Mathimani","doi":"10.1007/s10811-024-03303-0","DOIUrl":"https://doi.org/10.1007/s10811-024-03303-0","url":null,"abstract":"<p>Microalgae are photosynthetic microorganisms that have the capacity to synthesize a diverse range of biomolecules depending on their phylogenetic classification. Microalgae hold promise as sustainable and renewable energy resources; however, their full potential remains untapped due to the costliness and time-intensive nature of downstream processing. The objective of this study is to overcome these challenges by utilizing a single processing unit for the extraction of three major biomolecule classes namely, lipids, proteins, and carbohydrates. These biomolecules of marine <i>Chlorella</i> sp. NITT 02 were recovered in a single extraction process using the triphasic system. The parameters defining the triphasic system were optimized for the maximum recovery of biomolecules using Response Surface Methodology (RSM) coupled Genetic Algorithm (GA) optimization. The simultaneous complete recovery of lipids with 86.46 % of total fatty acids, 97 % protein recovery and 97.1 % carbohydrate recovery were obtained with i) 2.5:1 of t-butanol:culture volume, ii) 70 % t-butanol, iii) 32.9 % ammonium sulfate, and iv) 55 min sonication time. All three extracted biomolecule classes were characterized using FTIR, GC-MS, UV-Visible spectrophotometry, and XRD. From the current investigation, the recovery efficiency of the triphasic system is higher than the conventional sequential extraction of biomolecules and the time taken for extraction is 1.25 h with energy consumption of 0.7833 kWh. Hence, the adoption of this biorefinery approach is suitable for making microalgae commercially viable for producing valuable products from the biomolecules.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"31 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141585134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Staining to machine learning: An emerging technology for determination of microalgal cell viability","authors":"Taehee Kim, Biswajita Pradhan, Jang-Seu Ki","doi":"10.1007/s10811-024-03274-2","DOIUrl":"https://doi.org/10.1007/s10811-024-03274-2","url":null,"abstract":"<p>Microalgae are unicellular photosynthetic microorganisms typically found in aquatic environments and they play a vital role in the global carbon and energy cycles. Discrimination of dead and live cells is an important factor in microalgae research and environmental monitoring. Numerous research on the effects of various microalgae has been conducted concerning cell viability. Recently, dyes such as Trypan Blue (TB), Evans Blue (EB), and Neutral Red (NR) have been employed to assess the viability of microalgae. Existing approaches for identifying dead and living microalgal cells all have flaws, such as the requirement for staining and pre-treatment. A machine learning method was created to distinguish the living and dead microalgal cells by using of a digital holography microscopy, and the accuracy of this technique was greater. The machine learning method offers a new way of studying both freshwater and marine microalgal cell cultures. This review focuses on the existing methods and emerging technology for determining dead and living microalgae cells. This review work will enlighten the new research for the detection of live or dead microalgae.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"39 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141585135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal variation in biomass availability and biochemical composition of Gayralia brasiliensis (Ulotrichales, Chlorophyta) from Shirgaon estuary west coast of India","authors":"Mitesh Grewal, Kanchan Sambwani, Kanchan Udata, Murali Krishna Paidi, Tejal Gajaria, V. Veeragurunathan, Monica Gajanan Kavale","doi":"10.1007/s10811-024-03295-x","DOIUrl":"https://doi.org/10.1007/s10811-024-03295-x","url":null,"abstract":"<p>The present study investigated the impact of seasonal variation on biomass availability, proximate composition, fatty acid profile, minerals content, pigments, moisture content, and elemental composition of an edible green alga <i>Gayralia brasiliensis</i> collected from Shirgaon estuary Maharashtra, west coast of India. Additionally, the physico-chemical parameters of the Shirgaon estuary were analyzed and correlated with biomass abundance and biochemical composition of <i>G</i>. <i>brasiliensis</i>. The findings of the present study demonstrated a significant variation in the physico-chemical parameters of seawater throughout the seasons. During the summer season, the biomass abundance (258.23 ± 23.06 g m^-2 FW), and the total protein content (11.12 ±1.37 % DW) were found highest while the total lipid content declined considerably. Nonetheless, the contents <i>n-6</i> and <i>n-3</i> PUFA increased significantly over the winter season. The concentration of micro-elements was highest during the winter season while the macro-elements found plenty during the monsoon season. The correlogram analysis revealed that biomass, total protein, total carbohydrate, chlorophyll pigments, and tissue C, H, N, and S were positively correlated with each other throughout all three seasons. Based on Pearson corelation analysis it is confirmed that among the environmental parameters, irradiance and temperature were found the most limiting factors for the growth of <i>G</i>. <i>brasiliensis</i>. Further, dissolved inorganic nitrate significantly influence the growth of <i>G</i>. <i>brasiliensis</i> negatively. Overall, the results of this study imply that <i>G</i>. <i>brasiliensis</i> should be harvested for desired metabolites during the respective seasons. The findings of the present study are expected to be extremely valuable for future cultivation strategies and edible applications of <i>G</i>. <i>brasiliensis</i> in India.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"9 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141570859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Cyanobacteria (Nostoc sp.) immobilized rGO/PPy/ ITO-PET bio-anode for enhanced electrocatalytic and energy conversion for a Photo-bio-electrochemical cell","authors":"Pinki Choudhary, Neha Thakur, Sunita Mishra","doi":"10.1007/s10811-024-03305-y","DOIUrl":"https://doi.org/10.1007/s10811-024-03305-y","url":null,"abstract":"<p>This study aimed to develop anode with improved performance for potential use in energy applications, particularly in bio-photovoltaic applications. The study comprises the chemical synthesis of a conducting nanocomposite based on reduced graphene oxide and polypyrrole (rGO/PPy) by incorporating PPy into the rGO sheets along with the addition of an aerogel synthesis phase to improve the composite's overall characteristics. A comparative electrochemical analysis was conducted on cyanobacteria (<i>Nostoc</i> sp.) immobilised ITO-PET and modified rGO/PPy/ITO-PET anodes to investigate the photocurrent output of both. The rGO/PPy nanocomposite was further used to develop a cyanobacteria immobilised biofuel cell anode, and the electrochemical characterization of the fabricated bio-anode (rGO/PPy/ITO-PET) was carried out in a lab-made rudimentary electrochemical cell for the bio-electrocatalytic photolysis of water (light) and oxidation of stored organic matter (night). The results show that the modified bio-anode, for the bio-electrocatalytic reaction in the photo-bio-electrochemical cell configuration, attained a maximum current density of 0.132 mA cm^-2 in light, and 0.069 mA cm^-2 in dark at 0.0 V, and 0.375 mA cm^-2 in light, and 0.207 mA cm^-2in dark at an applied voltage of 1.45 V. Therefore, the electrocatalytic photolysis and oxidation of organic materials were accomplished by the proposed bio-anode via the direct electron transfer mechanism. The amperometric photocurrent response of the developed bio-electrode remained relatively stable for approximately 10 days in the rudimentary designed bio-electrochemical cell. The study demonstrates the potential of rGO/PPy/ITO-PET based bio-electrode for possible application in developing the bio-photovoltaic cells for energy generation.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"37 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-07-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141570860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryopreservation of vegetative thalli of Ulva species","authors":"Clara Simon, Antoine Fort, Ronan Sulpice","doi":"10.1007/s10811-024-03300-3","DOIUrl":"https://doi.org/10.1007/s10811-024-03300-3","url":null,"abstract":"<p>Sea lettuce (<i>Ulva</i>) is a genus of green macroalgae present along all the coasts of the world's oceans. It represents about 100 species with diverse habitats. Inter- and intra-species natural variation is very large, both in terms of growth characteristics and biomass biochemical composition. As a result, <i>Ulva</i> biomass has a wide range of applications and strain selection can achieve significant increases in yield(s). Establishing solid, long term and cost-effective methodologies for the conservation of <i>Ulva</i> genetic diversity is then required to safeguard and reuse selected strains. Here, we report a cryopreservation-based protocol for the long-term preservation of foliose <i>Ulva</i> strains. Strains from seven different <i>Ulva</i> species were cryopreserved for 15 and/or 120 days in liquid nitrogen, and of the 3 replicates cryopreserved, at least one survived, allowing us to successfully recover all strains. On average, among all specimen cryo-preserved, 82% of them survived and grew post cryo-preservation.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"38 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141570861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Internal and external spectral light conversion amplifying growth/bio-products formation of Dunaliella salina","authors":"Mojgan Mohebi Najafabadi, Fereshteh Naeimpoor","doi":"10.1007/s10811-024-03304-z","DOIUrl":"https://doi.org/10.1007/s10811-024-03304-z","url":null,"abstract":"<p>Rhodamine-6-G (R6G) was used to investigate the effect of internal/external light conversion on <i>Dunaliella salina</i> in a 1000 mL double-layer flat plate photobioreactor (DLF-PBR) and a 900 mL cylindrical photobioreactor (C-PBR). External spectrum conversion was examined in the DLF-PBR where the outer layer was filled with four different dye solutions (water/ethanol as solvents, dye at 25/50 ppm) to select the appropriate dye level and solvent type. The highest biomass (2.1 g L^-1) and productivity (116 mg L^-1day^-1) were obtained at low dye level in ethanol, while the highest productivities of storage materials (lipid:7.5, starch:18.73 mg L^-1day^-1) belonged to high level of dye in water. To benefit from conventional bioreactors with lower cost, internal conversion was carried out in C-PBR by addition of dye into medium aiming to compare growth and product formation with/without spectrum conversion. The highest level of biomass (3.49 g L^-1) was achieved by light conversion in C-PBR. Additionally, the effect of adaption of cells to dye was investigated in the C-PBR. A long lag-phase was observed in the case of non-adapted cells while this considerably reduced in the case of adapted cells. Moreover, a two-stage cultivation was studied in the C-PBR in which mixed red-blue LEDs exposure allowing enhanced growth without dye was used in the 1^st-stage and this was followed by addition of R6G and shifting to fluorescent exposure in the 2^nd-stage, which resulted in 146 and 293% higher productivities of lipid and starch, respectively, compared to control. The highest cell contents of lipid and starch (9.91, 22.15% g g^-1DCW) also corresponded to this strategy.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"6 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141547099","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Response of maize to seed priming with seaweed extracts and plant growth promoting rhizobacteria","authors":"Grishma Gandhi, Vijay Anand K. Gopalakrishnan, Arup Ghosh","doi":"10.1007/s10811-024-03306-x","DOIUrl":"https://doi.org/10.1007/s10811-024-03306-x","url":null,"abstract":"<p>Plant growth-promoting rhizobacteria (PGPR) and seaweed extracts (SWE) individually improve crop performances, but the response of crops upon their combined use is less understood. A field experiment on maize was carried out to evaluate the effect of seed priming with PGPR and undiluted SWE on the growth, yield, and nutritional quality of maize and soil properties. A split-plot design comprising 15 treatment combinations was laid out with SWE as the main factor (no SWE, <i>Kappaphycus alvarezii</i> and <i>Sargassum cinctum</i> extract, and PGPR as sub-plot factor (no PGPR, <i>Bacillus megaterium</i>, <i>B. subtilis</i>, <i>Paenibacillus polymyxa</i> and <i>Azotobacter chroococcum</i>). The composition and properties of SWE and PGPR were assessed. Without SWE, seed priming with all PGPR enhanced cob yield, the maximum improvement (32%) over no PGPR being recorded with <i>A. chroococcum</i> treatment. However, SWE priming along with PGPRs had no additional yield advantage compared to PGPR used alone. In the presence of either SWEs, <i>A. chroococcum</i> treatment was superior to other PGPR for most of the crop parameters like yield, carbohydrates and micro-nutrients in grains. The higher nutrient uptake agreed well with soil nutrient status after harvest. There were no deleterious effects on any parameters due to co-priming with <i>K. alvarezii</i> or <i>S. cinctum</i> extract with <i>A. chroococcum</i>, which was not the case with <i>B. subtilis</i> treatment where there was a significant decrease in growth and yield parameters despite evidence of increasing bacterial colony count. The study suggests the need for assessing optimal SWE doses for combinatorial PGPR treatments.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"47 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141547097","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alleviating the water deficit stress effect on Zea mays L. using an extract of the seaweed Sargassum boveanum","authors":"Farnaz Alasvandyari, Batool Mahdavi, Asghar Rahimi","doi":"10.1007/s10811-024-03282-2","DOIUrl":"https://doi.org/10.1007/s10811-024-03282-2","url":null,"abstract":"<p>In this study a liquid extract from the seaweed <i>Sargassum boveanum</i> (LE_Sb) was prepared using four different methods and solvents. The optimal method, where the extract was prepared using microwave digestion, was selected, and its spray solution was applied to maize (SC704) plants at different concentrations (0.0%, 1.0%, 1.5%, 2.0%, and 2.5% LE_Sb (_V/_V)) and three irrigation levels: non-stress (80% of field capacity (FC), 60% FC, and 40% FC). Water stress (40% and 60% FC) limited plant growth by disrupting physiological and biochemical functions. However, the concentration of 1.5% LE_Sb demonstrated a significant improvement in various traits compared to the 0.0% LE_Sb. This improvement included an increase in shoot dry weight by 15.46%, 27.55%, and 57.52% under conditions of 80%, 60%, and 40% FC. Additionally, there was an increase in the activity of antioxidant enzymes, such as superoxide dismutase (SOD), by 13.27%, 30.42%, and 32.19% under conditions of 80%, 60%, and 40% FC, respectively, which could effectively reduce malondialdehyde (MDA) levels. At the concentration of 1.5% there was an increase in proline content by 20.07%, 19.50%, and 10.40% under conditions of 80%, 60%, and 40% FC, respectively, as well as an increase in soluble sugar content under conditions of 60% and 40% FC compared to 0.0% LE_Sb. The relative water content (RWC) also improved with the 1.5% LE_Sb under 60% and 40% FC conditions, by 29.23% and 25.97%, respectively, compared to 0.0% LE_Sb. These results indicate that a concentration of 1.5% LE_Sb can effectively alleviate the negative effects of water stress on maize plants.</p>","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":"23 1","pages":""},"PeriodicalIF":3.3,"publicationDate":"2024-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141547098","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}