Journal of Biotechnology and Biomedicine最新文献_第5页

Recent Developments in High Spatial-Temporal Image-Based Tracking of Proteins in Subcellular Spatial Proteomics Applications 基于高时空图像的亚细胞空间蛋白质组学蛋白质跟踪研究进展

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280092

Wenfa Ng

{"title":"Recent Developments in High Spatial-Temporal Image-Based Tracking of Proteins in Subcellular Spatial Proteomics Applications","authors":"Wenfa Ng","doi":"10.26502/jbb.2642-91280092","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280092","url":null,"abstract":"Dawn of the omics revolution in biological sciences meant that we seek to understand more and in greater detail the molecular constituents of cells and biological systems. While we have gained significant insights from conventional omics tools, we now seek to understand the spatial dimensions of the data where subcellular localisation may impact on cellular physiology and phenotype. This review paper seeks to address current questions in the new field of image-based spatial proteomics as well as outline future challenges of the field. At first glance, spatial proteomics offers enormous potential to expand our understanding of different cell types in different disease and cell states. But limitations in types of fluorophores and issues with spectral overlap significantly hampers the practical implementation of the technique. On the other hand, while we have super-resolution microscopy techniques such as STED, PALM and STORM able to achieve 10 to 20 nm spatial resolution in single molecule localisation, problems with slow image acquisition limits high temporal resolution tracking of multiple protein targets in live cell imaging. Hence, the field of spatial proteomics is a mix of promises and challenges where we could image, in multi-colour, upwards of 10 well-chosen proteins that could inform on the molecular mechanisms of selected biological processes, but, at present, the method could not tackle larger scale questions. In essence, current implementation of image-based spatial proteomics is useful, but it is unable to fulfil the mission of large-scale projects such as the Human Protein Atlas or Human Cell Atlas. Future challenges in the field includes the development of more fluorophores (especially photo switchable and photoactivable ones) for single molecule localisation microscopy, as well as seeking to improve temporal resolutions to the sub-millisecond range.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82739197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Home Monitoring for Fever: An Inexpensive Screening Method to Prevent Household Spread of COVID-19 家庭发热监测:预防COVID-19家庭传播的一种廉价筛查方法

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280101

Justin Kim, Marcus A Threadcraft, Wei Xue, Sijie Yue, Richard P Wenzel, Frederick S Southwick

{"title":"Home Monitoring for Fever: An Inexpensive Screening Method to Prevent Household Spread of COVID-19","authors":"Justin Kim, Marcus A Threadcraft, Wei Xue, Sijie Yue, Richard P Wenzel, Frederick S Southwick","doi":"10.26502/jbb.2642-91280101","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280101","url":null,"abstract":"The COVID-19 pandemic surge has exceeded testing capacities in many parts of the world. We investigated the effectiveness of home temperature monitoring for early identification of COVID-19 patients. Study Design: We compared home temperature measurements from a convenience sample of 1180 individuals who reported being test positive for SARS-CoV-2 to an age, sex, and location matched control group of 1249 individuals who had not tested positive. Methods: All individuals monitored their temperature at home using an electronic smartphone thermometer that relayed temperature measurements and symptoms to a centralized cloud based, de-identified data bank. Results: Individuals varied in the number of times they monitored their temperature. When temperature was monitored for over 72 hours fever (> 37.6°C or 99.7°F or a change in temperature of > 1°C or 1.8°F) was detected in 73% of test positive individuals, a sensitivity comparable to rapid SARS-CoV-2 antigen tests. When compared our control group the specificity of fever for COVID-19 was 0.70. However, when fever was combined with complaints of loss of taste and smell, difficulty breathing, fatigue, chills, diarrhea, or stuffy nose the odds ratio of having COVID-19 was sufficiently high as to obviate the need to employ RTPCR or antigen testing to screen for and isolate coronavirus infected cases. Conclusions: Our findings suggest that home temperature monitoring could serve as an inexpensive convenient screen for the onset of COVID-19, encourage earlier isolation of potentially infected individuals, and more effectively reduce the spread of infection in closed spaces.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135959527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Immunomodulatory Effect of Electromagnetic Field in the Treatment of Traumatic Brain Injury. 电磁场在治疗创伤性脑损伤中的免疫调节作用。

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 Epub Date: 2023-02-01 DOI: 10.26502/jbb.2642-91280069

Tye Patchana, Devendra K Agrawal, David Connett, David Baron, Dan Miulli

引用次数: 0

Automated Synthesis Method to Produce the PET Tracer [68Ga]Ga-FAPI-46 for Clinical Applications: Development, Optimization and Validation 用于临床应用的PET示踪剂[68Ga]Ga-FAPI-46的自动合成方法:开发、优化和验证

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280096

S. Migliari, M. Scarlattei, G. Baldari, Livia Ruffini

引用次数: 0

A Probiotic to Improve Sleep Quality during COVID-19 Pandemic: A Questionnaire Study 一项益生菌改善COVID-19大流行期间睡眠质量的问卷研究

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280073

A. Horvath, J. Wagner-Skacel, V. Stiegelbauer, V. Stadlbauer

{"title":"A Probiotic to Improve Sleep Quality during COVID-19 Pandemic: A Questionnaire Study","authors":"A. Horvath, J. Wagner-Skacel, V. Stiegelbauer, V. Stadlbauer","doi":"10.26502/jbb.2642-91280073","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280073","url":null,"abstract":"Sleep quality has been related to the gut microbiome and probiotics were shown to improve sleep quality, especially in people under physical or mental stress. COVID-19 results in such mental stress, leading to sleeping disorders, especially in people working in health care. A large dataset of quality of life data from people working in pharmacies or doctors´ offices was collected before and after intake of a free sample of a multi-species probiotic during a marketing survey. 9841 anonymous datasets were available for analysis. Group differences and univariate and multivariate logistic regression analysis was performed. COVID-19 related data were obtained from publicly available datasets and matched to quality of life data via the postal code of the participants. We aimed to relate self-reported quality of life, especially sleep quality data from a marketing survey to objective criteria of COVID-19, such as incidence and mortality figures at the time of the survey. The intake of only a two-week course of a multi-species probiotic markedly improved sleep quality, mental health, vitality, stress resistance and performance. Improvement in the different categories of quality of life was influenced by sex, age, initial intestinal complaints, the initial stress level, the initial sleep quality, initial vitality and mental health. Quality of life was reduced during COVID-19 compared to pre-pandemic values of the general population. We also found some associations of changes in quality of life in relation to COVID-19 incidence rates in employees of pharmacies and doctors´ offices, however to a lesser extent than expected. Although not derived from a clinical trial, this analysis of marketing survey data provides evidence that there is a link between the gut microbiome, sleep and quality of life suggesting that gut microbiome modulation could be a valuable therapeutic approach in sleeping disorders.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82053721","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Double Trouble Mutations Underlie Mitochondrial Dynamics Disorders in a Severe Form of Charcot-Marie-Tooth Disease 双重麻烦突变是严重形式的沙科-玛丽-图斯病线粒体动力学紊乱的基础

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280109

Angela Puma, Samuel Guilbault, Emmanuelle C Genin, Christophe Duranton, Isabelle Rubera, Nathalie Bonello-Palot, Véronique Paquis-Flucklinger, Sabrina Sacconi, Saïd Bendahhou

{"title":"Double Trouble Mutations Underlie Mitochondrial Dynamics Disorders in a Severe Form of Charcot-Marie-Tooth Disease","authors":"Angela Puma, Samuel Guilbault, Emmanuelle C Genin, Christophe Duranton, Isabelle Rubera, Nathalie Bonello-Palot, Véronique Paquis-Flucklinger, Sabrina Sacconi, Saïd Bendahhou","doi":"10.26502/jbb.2642-91280109","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280109","url":null,"abstract":"Charcot-Marie-Tooth disease type 2A (CMT2A) is an inherited axonal peripheral neuropathy mainly caused by mutations in the mitofusin 2 (MFN2) gene encoding for the MFN2 protein, a GTPase involved in the mitochondrial dynamics and bioenergetics. We identified a novel mutation, that was inherited from her mother, in the MFN2 protein (Met234Ile) in a Charcot-Marie-Tooth patient. She has also a variant of uncertain significance (Thr36Ala), in the PMP22 gene, encoding for peripheral myelin protein 22, inherited from her father. The patient presented severe sensorimotor neuropathy with early onset. The mother presented a distal muscle atrophy in the legs, the father was asymptomatic. To show the pathogenicity of these mutations, we characterized the structure, adenosine triphosphate (ATP) content, bioelectric characteristics and functions of mitochondria in cultured primary fibroblasts obtained from the proband and her parents. Under normal culture conditions, mitochondria showed normal morphology. Under oxidative stress conditions, ATP production was reduced and the proband cells showed a decrease of the mitochondrial fusion with small connected networks and a decrease of the mitochondrial volume. The alteration of the mitochondrial network only when cells are challenged in aerobiosis testifies to the fragility of mitochondria, which are unable to meet the metabolic needs of neurons. Interestingly, fibroblasts derived from the two parents did not show any change. These results support the hypothesis that the mutation in the MFN2 gene altering mitochondrial bioenergetics and fusion causes axonal sensory-motor neuropathy. We speculate that PMP22 may promote mitochondrial dysfunction, myelin having a role in mitochondrial metabolism.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136257961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Quantised Cyclin-Based Cell Cycle Model 基于周期蛋白的细胞周期定量模型

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280098

Chris Emerson, Lindsey Bennie, Niall M. Byrne, Dermot Green, Fred Currell, Jonathan A. Coulter

引用次数: 0

Accelerating High-Performance Classification of Bacterial Proteins Secreted Via Non-Classical Pathways: No Needing for Deepness 加速通过非经典途径分泌的细菌蛋白质的高效分类:不需要深度

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280113

Luiz Gustavo de Sousa Oliveira, Gabriel Chagas Lanes, Anderson Rodrigues dos Santos

引用次数: 0

Perception and knowledge of artificial intelligence in healthcare, therapy and diagnostics: A population-representative survey 人工智能在医疗保健、治疗和诊断中的感知和知识:一项人口代表性调查

Journal of Biotechnology and Biomedicine Pub Date : 2022-12-06 DOI: 10.1101/2022.12.01.22282960

Cornelius G Wittal, Doerte Hammer, Farina Klein, Joachim Rittchen

引用次数: 0

Bee Venom Phospholipase A2 reduces Tau phosphorylation through inhibition of GSK3β expression 蜂毒磷脂酶A2通过抑制GSK3β表达减少Tau磷酸化

Journal of Biotechnology and Biomedicine Pub Date : 2022-02-21 DOI: 10.21203/rs.3.rs-1356322/v1

Seung Sik Yoo, S. Han, J. Yun, I. Yeo, Hyeon Joo Ham, Yeon-Jung Kim, D. Son, E. Park, H. Rhee, D. Hwang, Pil-Hoon Park, D. Choi, Won-Kyu Lee, Jin T. Hong

{"title":"Bee Venom Phospholipase A2 reduces Tau phosphorylation through inhibition of GSK3β expression","authors":"Seung Sik Yoo, S. Han, J. Yun, I. Yeo, Hyeon Joo Ham, Yeon-Jung Kim, D. Son, E. Park, H. Rhee, D. Hwang, Pil-Hoon Park, D. Choi, Won-Kyu Lee, Jin T. Hong","doi":"10.21203/rs.3.rs-1356322/v1","DOIUrl":"https://doi.org/10.21203/rs.3.rs-1356322/v1","url":null,"abstract":"\u0000 Background\u0000 Alzheimer's disease (AD) is characterized by to neuronal cell death and neuroinflammation. Neurofibrillary tangle (NFTs) is one of the neuropathological hallmarker of AD. Also our previous study indicated that bee venom leads to neuroprotective effects in a lipopolysaccharide (LPS)-induced AD mouse model. Thus, in this study we investigated whether that phospholipase A2 (PLA2) reduces tau phosphorylation and neuroinflammation, and thus ameliorates AD development.\u0000Results\u0000 To validate pathological activities in in vivo, we examined of the inhibitory effect of bvPLA2 on memory loss and tau phosphorylation as well as neuroinflammation by subcutaneous injection of bvPLA2 (0.5 mg/kg) to Tg2576 mice. For in vitro study, we examined the effect of bvPLA2 on cell death, tau pathology and neuroinflammation by treatment of bvPLA2 in LPS-activated PC12 cells. Our study showed that bvPLA2 mitigated memory impairment and spatial memory in Tg2576 mice, Agreed with the memory improvement, tau level and phosphorylation of tau were decreased by bvPLA2 treatment. Expression level of pro-inflammatory cytokines and inflammation-related proteins were also decreased in the brain of bvPLA2-treated Tg2576 mice.\u0000Conclusions\u0000 Consideration of reduced tau level and phosphorylation of tau, GSK3β phosphorylation was studied. Phosphorylated GSK3β on Ser9 was significantly increased by treatment of bvPLA2, but a phosphorylated GSK3β on Tyr216 was significantly decreased in the Tg2576 mice brains. These data thus indicate that bvPLA2 prevents memory impairment through reduction of tau phosphorylation.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"87 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75421435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0