Journal of Biotechnology and Biomedicine最新文献_第4页

Extracellular Matrix Glycoprotein Thrombospondin 1: An Overlooked Pathological Mediator in Vasculopathy of Systemic Sclerosis-Secondary Raynaud’s Phenomena 细胞外基质糖蛋白-血小板反应蛋白1:在系统性硬化症-继发性雷诺现象血管病变中被忽视的病理介质

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280093

Zulfa Allaf, Megan Yao

{"title":"Extracellular Matrix Glycoprotein Thrombospondin 1: An Overlooked Pathological Mediator in Vasculopathy of Systemic Sclerosis-Secondary Raynaud’s Phenomena","authors":"Zulfa Allaf, Megan Yao","doi":"10.26502/jbb.2642-91280093","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280093","url":null,"abstract":"Systemic sclerosis (SSc) is an autoimmune connective tissue disorder characterized by a widespread vasculopathy, autoimmunity, and fibrosis of the skin and internal organs such as the lung and kidneys. Raynaud's phenomenon is the earliest recognized symptom of SSc. Unlike the primary Raynaud's disease, the condition responds well to management with non-pharmacological measures and pharmacological agents, Raynaud's phenomenon presented complex and progressive vasculopathy in the superficial vasculature embedded under the skin. The mechanisms underlying the development and progress of Raynaud's phenomenon are still unclear. Repeated Raynaud's phenomenon attacks are characteristic of ischemia-reperfusion episodes with different durations. What occurred to the superficial vascular bed highly possibly replicates at the vasculature in the vulnerable internal organ(s) at a later time and a distinctive pace. Elevated extracellular matrix glycoprotein thrombospondin 1 (TSP1) levels are found in circulation and throughout the skin in patients with SSc. TSP1-mediated vascular pathologies have been extensively investigated in multiple conditions, including hypertension, pulmonary arterial hypertension, and renal ischemia-reperfusion injury. TSP1 vasculopathy in these conditions is evidenced by a variety of function-modulating and vascular remodeling effects, specifically enhancing vasoconstrictive tone, platelet hyperaggregation, and inflammatory cell infiltration, inducing capillary rarefaction and promoting intimal thickening. In addition, the well-known anti-angiogenic property of TSP1 significantly impairs the self-repair and self-renewal capacity of growth factor-or stem cell-based regenerative therapies. Thanks to the growing knowledge of TSP1 vasculopathy in affected internal organs and adverse effects on regrowth, it is proposed that pathological levels of TSP1 may exert a similar pattern of multifaceted effects on the vasculature in SSc and regenerative therapies under investigation.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"89 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83871546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of Handing a Written Document to Share Concerns and Values with Physicians When Making Decisions about Starting Drug Treatment among Patients: A Pre-post Quasi-Experimental Study with Propensity Score Matching 在决定患者开始药物治疗时，提供书面文件与医生分享关注和价值观的影响:一项倾向评分匹配的前后准实验研究

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280086

S. Bito, Tomomi Iioka, Yasuhiro Yamada, E. Hiraoka, Taiju Miyagami

{"title":"Effect of Handing a Written Document to Share Concerns and Values with Physicians When Making Decisions about Starting Drug Treatment among Patients: A Pre-post Quasi-Experimental Study with Propensity Score Matching","authors":"S. Bito, Tomomi Iioka, Yasuhiro Yamada, E. Hiraoka, Taiju Miyagami","doi":"10.26502/jbb.2642-91280086","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280086","url":null,"abstract":"Background: There is lim ited evidence on the effectiveness of decision aids in promoting clinicians' understanding of patients' subjective perceptions. The aim of this study was to assess the impact of using a standard patient-completed template to express patients' views, preferences or concerns to their clinicians in a clinical decision-making setting on patients' perceived decisional conflict and post-decision regret. Methods: A pre-post quasi-experimental study with a six-month control period followed by a 12-month intervention period was conducted. Participants were recruited from six teaching hospitals and two clinics in Japan. The target population included 150 patients with diabetes mellitus, hypertension, and/or dyslipidaemia whose physicians had recently suggested drug treatment as a medical option. In the control period, a general informed consent booklet was distributed, and in the intervention period, a shared decision-making template was also provided. Patients were asked to complete the template, which was then attached to their electronic medical records. Results: Two months after enrolment, the decision conflict and regret scales were mailed to patients. Three months after enrolment, the decision status for starting drug treatment and the concordance between the patients' decision statuses and the initial medical recommendations were observed. Seventy-nine and seventy-one participants assigned to the control and intervention groups were enrolled. Fifty-five patient pairs generated by propensity score matching were analysed. No significant difference was observed between the two groups in the subscale scores of the decision conflict scale and the decision regret scale. The relative risk of patients with a decision status of ‘still considering’ starting drug treatment was 2.2 (95% CI, 1.02-4.9) in the intervention group. The concordance","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88675126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heideggerian Hermeneutic Phenomenological Study of Lived Experiences of Nursing Care during Childbirth 分娩期间护理生活经验的海德格尔解释学现象学研究

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280079

Nicholas Yakubu Danzima, Bismark Nantomah, Vivian Kapio Abem

引用次数: 0

Short-Chain Fructooligosaccharides Improve Gut Microbiota Composition in Patients with Type 2 Diabetes. A randomized, Open-Label, Controlled Pilot Clinical Trial 短链低聚果糖改善2型糖尿病患者肠道菌群组成一项随机、开放标签、对照的先导临床试验

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280087

G. García, D. Martínez, Josanne Soto, L. Rodríguez, M. Nuez

引用次数: 1

Outpatient Administration of Blinatumomab Infusion and Hematopoietic Stem Cell Transplantation as Treatment for Patients with Refractory Acute Lymphoblastic Leukemia in a Pediatric Oncologic Center in Mexico 在墨西哥儿科肿瘤中心，布利纳单抗输注和造血干细胞移植治疗难治性急性淋巴细胞白血病的门诊管理

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280106

Godoy-Fernández JF, Cubría-Juárez MP, Zapata-Tarrés M, Taveras-Rodríguez O, Briseño-Rebollar EV

{"title":"Outpatient Administration of Blinatumomab Infusion and Hematopoietic Stem Cell Transplantation as Treatment for Patients with Refractory Acute Lymphoblastic Leukemia in a Pediatric Oncologic Center in Mexico","authors":"Godoy-Fernández JF, Cubría-Juárez MP, Zapata-Tarrés M, Taveras-Rodríguez O, Briseño-Rebollar EV","doi":"10.26502/jbb.2642-91280106","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280106","url":null,"abstract":"Acute Lymphoblastic Leukemia (ALL) is the most common pediatric cancer and the most common subtype of pediatric leukemia, the high toxicity of chemotherapy, the relapse and refractory disease of many patients require the development of novel therapeutic approaches such as targeted immunotherapy in combination with Hematopoietic Stem Cell Transplantation (HSCT). These new therapeutic approaches must be taken in count to revolutionize the management of numerous ALL subtypes, improve treatment outcomes and maintain low toxicity without losing the effectiveness of the treatment. Our retrospective, longitudinal, observational, descriptive study evaluated a novel therapeutic approach in a Pediatric Oncologic Center in Mexico, the outpatient administration of blinatumomab infusion in patients with relapsed/refractory acute lymphoblastic leukemia (R/R ALL). The study demonstrated that 100% of the patients went into remission after Blinatumomab infusion and there was no mortality associated.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135959523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterozygote Germline Mutations in Homologous Recombination Core Genes Can Predict for Pathologic Complete Response in Early Triple Negative Breast Cancer 同源重组核心基因杂合子种系突变可预测早期三阴性乳腺癌的病理完全缓解

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280110

C Fontaine, S De Brakeleer, E Teugels, V Renard, H Van den Bulck, P Vuylsteke, P. Glorieux, C Dopchie, S Joris, L Decoster, A Awada, K Punie, H Wildiers, J De Grève

引用次数: 0

A Cost-Benefit Analysis of Bivalent Covid-19 Vaccines 新型冠状病毒二价疫苗的成本效益分析

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280116

Dan-Yu Lin, Yangjianchen Xu, Donglin Zeng, Shadia Khan Sunny

引用次数: 0

The ERK Signaling Cascade Inhibits Gonadotropin-Stimulated Steroidogenesis. ERK信号级联抑制促性腺激素刺激的类固醇生成。

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280066

Rony Seger, Tamar Hanoch, Revital Rosenberg, Ada Dantes, Wolfgang E Merz, Jerome F Strauss, Abraham Amsterdam

{"title":"The ERK Signaling Cascade Inhibits Gonadotropin-Stimulated Steroidogenesis.","authors":"Rony Seger, Tamar Hanoch, Revital Rosenberg, Ada Dantes, Wolfgang E Merz, Jerome F Strauss, Abraham Amsterdam","doi":"10.26502/jbb.2642-91280066","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280066","url":null,"abstract":"<p><p>The response of granulosa cells to Luteinizing Hormone (LH) and Follicle- Stimulating Hormone (FSH) is mediated mainly by cAMP/protein kinase A (PKA) signaling. Notably, the activity of the extracellular signal-regulated kinase (ERK) signaling cascade is elevated in response to these stimuli as well. We studied the involvement of the ERK cascade in LH- and FSH-induced steroidogenesis in two granulosa-derived cell lines, rLHR-4 and rFSHR-17, respectively. We found that stimulation of these cells with the appropriate gonadotropin induced ERK activation as well as progesterone production downstream of PKA. Inhibition of ERK activity enhanced gonadotropin-stimulated progesterone production, which was correlated with increased expression of the Steroidogenic Acute Regulatory Protein (StAR), a key regulator of progesterone synthesis. Therefore, it is likely that gonadotropin-stimulated progesterone formation is regulated by a pathway that includes PKA and StAR, and this process is down-regulated by ERK, due to attenuation of StAR expression. Our results suggest that activation of PKA signaling by gonadotropins not only induces steroidogenesis but also activates down-regulation machinery involving the ERK cascade. The activation of ERK by gonadotropins as well as by other agents may be a key mechanism for the modulation of gonadotropin-induced steroidogenesis.</p>","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"6 1","pages":"1-12"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10035566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9246460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 27

Recent Developments in High Spatial-Temporal Image-Based Tracking of Proteins in Subcellular Spatial Proteomics Applications 基于高时空图像的亚细胞空间蛋白质组学蛋白质跟踪研究进展

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280092

Wenfa Ng

{"title":"Recent Developments in High Spatial-Temporal Image-Based Tracking of Proteins in Subcellular Spatial Proteomics Applications","authors":"Wenfa Ng","doi":"10.26502/jbb.2642-91280092","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280092","url":null,"abstract":"Dawn of the omics revolution in biological sciences meant that we seek to understand more and in greater detail the molecular constituents of cells and biological systems. While we have gained significant insights from conventional omics tools, we now seek to understand the spatial dimensions of the data where subcellular localisation may impact on cellular physiology and phenotype. This review paper seeks to address current questions in the new field of image-based spatial proteomics as well as outline future challenges of the field. At first glance, spatial proteomics offers enormous potential to expand our understanding of different cell types in different disease and cell states. But limitations in types of fluorophores and issues with spectral overlap significantly hampers the practical implementation of the technique. On the other hand, while we have super-resolution microscopy techniques such as STED, PALM and STORM able to achieve 10 to 20 nm spatial resolution in single molecule localisation, problems with slow image acquisition limits high temporal resolution tracking of multiple protein targets in live cell imaging. Hence, the field of spatial proteomics is a mix of promises and challenges where we could image, in multi-colour, upwards of 10 well-chosen proteins that could inform on the molecular mechanisms of selected biological processes, but, at present, the method could not tackle larger scale questions. In essence, current implementation of image-based spatial proteomics is useful, but it is unable to fulfil the mission of large-scale projects such as the Human Protein Atlas or Human Cell Atlas. Future challenges in the field includes the development of more fluorophores (especially photo switchable and photoactivable ones) for single molecule localisation microscopy, as well as seeking to improve temporal resolutions to the sub-millisecond range.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82739197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atopy and Food Allergies among Children Attending Outpatient Department of a Selected Tertiary Care Hospital in Dhaka City 达卡市某三级医院门诊儿童的特应性和食物过敏

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280088

Biplob Biswas, Wahida Khanam, D. Hossain, Mohammad Ahad Adnan, U. Datta, A. K. Naseruzzaman, Zamil Ahmed Manik, Tauhidul Islam, Kumar Datta, Ahmed Manik

{"title":"Atopy and Food Allergies among Children Attending Outpatient Department of a Selected Tertiary Care Hospital in Dhaka City","authors":"Biplob Biswas, Wahida Khanam, D. Hossain, Mohammad Ahad Adnan, U. Datta, A. K. Naseruzzaman, Zamil Ahmed Manik, Tauhidul Islam, Kumar Datta, Ahmed Manik","doi":"10.26502/jbb.2642-91280088","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280088","url":null,"abstract":"Background: Atopic dermatitis (AD), also called atopic eczema, is a common chronic or recurrent inflammatory skin disease and affects 15-20% of children. Objective: The aim of the study was to determine the proportion of atopy and food allergies among children attending outpatient department of a selected tertiary care hospital in Dhaka city. Methodology: This was a cross-sectional study. It was carried out on 384 children from June 2022 to December 2022 at pediatric outpatient department in Institute of child and mother health (ICMH), Dhaka, Bangladesh. The statistical evaluation of the results by using a window-based computer software program devised with Statistical Packages for Social Sciences (SPSS-24). Result: Total 384 children were enrolled among them Mean age was (1.15±0.43) years, 52% were female and 92.18% had a family history of atopy. Regarding symptoms 4.3% had lip or tongue swelling, 8.5% had difficulty in swallowing, 39.30% had urticaria, 13.7% had dyspnea and 5.1% had anaphylaxis. 82.8% of the respondents had mild illness, 11.98% had moderate illness and 5.21% had severe illness. 48.6% had Cow's Milk allergy, 19.5% to beef, 3.3% to egg ,2.6%to hilsa fish and prawn respectively and 22.3% to others. Mean Eosinophil count was (11.62±2.14), IgE was (747.40±314.2) IU and the skin prick test was positive 90.0%. Conclusion : The predominant sign of food allergy in young children was atopic dermatitis. Allergic conjunctivitis, oral allergy syndrome and respiratory distress appeared later in the progression of symptoms. Additionally, anaphylaxis is usually used to affect older children.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85938524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0