Journal of Biotechnology and Biomedicine最新文献

{"title":"Cellular Mechanisms of Electromagnetic Field in Traumatic Brain Injury.","authors":"Imran Siddiqi,&nbsp;Maxwell Marino,&nbsp;Devendra K Agrawal,&nbsp;Dave Baron,&nbsp;David A Connett,&nbsp;Daniel Miulli","doi":"10.26502/jbb.2642-91280075","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280075","url":null,"abstract":"<p><p>This paper presents a comprehensive review of the extant literature from 1980 through 2023 on the role and utility of Electromagnetic Fields (EMF) in the treatment of brain trauma and brain neuropathology resulting from disease. Brain trauma resulting from accident, injury and disease is a significant contributor to short and long-term morbidity, as well as a leading cause of mortality globally. To date, limited effective treatments strategies exist, and are focused primarily on symptom relief, not restoring primary preinjury function and structure. Much of the current clinical literature is based on retrospective case reports and limited animal model prospective trials exploring core etiology and alterations in post-injury clinical phenotypes. The current findings reported in the scientific literature suggest that electromagnetic therapy may hold promise as a potential non-invasive treatment for traumatic brain injury and neuropathology. Although promising, well designed clinical trials are needed to better determine its potential clinical effectiveness in this diverse patient population. Future trials will need to determine the impact of clinical variables, such as sex, age, type and extent of injury and pathology, pre-injury baseline health status and a comprehensive biopsychosocial assessment to determine a more effective personalized approach to patient care. Although initially showing promise, much work needs to be done.</p>","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"6 2","pages":"95-104"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310312/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9743321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cholesterol: An Important Determinant of Muscle Atrophy in Astronauts.","authors":"Hoangvi Le,&nbsp;Vikrant Rai,&nbsp;Devendra K Agrawal","doi":"10.26502/jbb.2642-91280072","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280072","url":null,"abstract":"<p><p>Since cholesterol is not routinely measured in astronauts before and after their return from space, there is no data on the role of blood cholesterol level in muscle atrophy and microgravity. Since the first moon landing, aerospace medicine became outdated and has not pushed boundaries like its rocket engineering counterpart. Since the 2019 astronaut twin study, there has yet to be another scientific breakthrough for aerospace medicine. Microgravity-induced muscle atrophy is the most known consequence of spaceflight. Yet, so far, there is no therapeutic solution to prevent it or any real efforts in understanding it on a cellular or molecular level. The most obvious reason to this unprecedented level of research is due to the small cohort of astronauts. With the establishment of private space industries and exponential recruitment of astronauts, there is more reason to push forward spaceflight-related health guidelines and ensure the safety of the brave humans who risk their lives for the progression of mankind. Spaceflight is considered the most challenging job and the failure to prevent injury or harm should be considered reckless negligence by the institutions that actively prevented sophistication of aerospace medicine. In this critical review, role of cholesterol is analyzed across the NASA-established parameters of microgravity-induced muscle atrophy with a focus on potential therapeutic targets for research.</p>","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"6 1","pages":"67-79"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10062007/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10348768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Molecular Pathogen Testing for Identifying the Etiology of Febrile Illness in Immunocompromised Children","authors":"Fabian JS van der Velden, Priyen Shah, Marie Voice, Emma Lim","doi":"10.26502/jbb.2642-91280100","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280100","url":null,"abstract":"Background: Diagnosing febrile illness in immunocompromised children at presentation to hospital remains a challenge. Serious bacterial infection can cause significant mortality and morbidity, but conventional diagnostics using culture-based technology results are often negative. Molecular pathogen testing might increase the yield of pathogen detection in this population, subsequently potentially altering clinical management and improving outcome.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"269 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135445759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Scientific Evidence for the Cancer Treatment of Human-Natured Infrared Radiation and Stimulated Electron Radiation","authors":"Enkhmaa Erdene-Ochir, Batsuuri Jamiyan, Munkhbaatar Chinbat","doi":"10.26502/jbb.2642-91280104","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280104","url":null,"abstract":"The treatments of surgery in the early stages of cancer, physical radiation and chemotherapy in severe metastases are now commonplace around the world. When the cancer has spread (metastasis) within the organ of its first formation and to other parts of the body, these methods can increase life expectancy by a maximum of five years. At the current level of scientific progress, no perfect cure for cancer has yet been found. Here, we demonstrate the result of our study of how Purevsuren Begzjav, a Mongolian citizen and healer, uses her natural bioenergy to completely cure late-stage cancer. It was found that B.Purevsuren’s palms (body) emit hot photons of stimulated electrons saturated with infrared light at 40°C to 45°C, which regenerate the patient’s bioplasma (energy) body and restrain the cancer foci and eradicate it. B.Purevsuren’s innate infrared energy reaches the organs and organ systems of the human body in appropriate doses, is harmless, and the level of her bioenergy parameters is quite stable. For example, the mean pre-treatment bioenergy area of the healer (¯X±S = 11549.3±672.3) is maintained at 98.4 percent compared to the post-treatment average (¯X±S = 11368.0±650.2). However, the mean pre-treatment bioenergy intensity of the healer (¯X±S = 75.42±1.48) is maintained at 99.6 percent compared to the post-treatment average (¯X±S = 75.14±1.53). The result of B. Purevsuren’s treatment is providing evidence that there is a scientific approach to cure cancer with photons of human-natured, high-temperature infrared energy-stimulated electrons completely. Trial registration (NIRT20121127), November 27, 2012.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"140 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135959522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Whole Exome Sequence of Pakistani Acute Lymphocytic Leukemia Patient from Pakhtuns Ancestry Reveal the Novel Genetic Variant Characterization in the GLDC Gene","authors":"Shahid Ullah, Alex Tonks, Maryam A Halawi, Alruwaili A M","doi":"10.26502/jbb.2642-91280103","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280103","url":null,"abstract":"Background: Acute Lymphoblastic Leukemia (ALL) is the most common malignant disease in children and often involves numerical chromosomal abnormalities, fusion genes, or minor localized deletions that are significant in the development of leukemia. Glycine Decarboxylase (GLDC) gene overexpression and mutation is associated with oncogenic activity in various cancers. However, the pathophysiological roles and structural consequences of GLDC in acute lymphocytic leukemia have not been investigated.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"41 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135959524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Discovery of Potential RAGE inhibitors using Receptor-Based Pharmacophore Modeling, High Throughput Virtual Screening and Docking Studies","authors":"Harbinder Singh, Devendra K Agrawal","doi":"10.26502/jbb.2642-91280112","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280112","url":null,"abstract":"Receptor for Advanced Glycation End products (RAGE) is a transmembrane receptor that can bind to various endogenous and exogenous ligands and initiate the inflammatory downstream signaling pathways. So far RAGE has been involved in various disorders including cardiovascular and neurodegenerative diseases, cancer, and diabetes. Blocking the interactions between RAGE and its ligands is a therapeutic approach to treat these conditions. In this context, we effectively utilized the receptor-based-pharmacophore modeling to discover structurally diverse molecular compounds having potential to effectively bind with RAGE. Two pharmacophore models were developed on V-domain of RAGE using Phase application of Schrodinger suite. The developed pharmacophoric features were used for screening of 1.8 million drug-like molecules downloaded from ChEMBL database. The molecules were scrutinized according to their molecular weight as well as clogP values. Phase screening was performed to find out the molecules that matched the developed pharmacophoric features that were further selected to analyze their binding modes using high-throughput virtual screening, extra precision docking studies and MM-GBSA ΔG binding calculations. These analyses provided ten hit RAGE inhibitory molecules that can bind to two different shallow binding sites on the V-domain of RAGE. Among the obtained compounds two compounds ChEMBL501494 and ChEMBL4081874 were found with best binding free energies that proved their receptor-ligand complex stability within their respective binding cavity on RAGE. Therefore, these molecules could be utilized for further designing and optimizing the future class of potential RAGE inhibitors.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134883918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Potential of \"Smart\" Exosomes in Peripheral Nerve Regeneration.","authors":"Rajiv Supra,&nbsp;Daniel R Wilson,&nbsp;Devendra K Agrawal","doi":"10.26502/jbb.2642-91280082","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280082","url":null,"abstract":"<p><p>Peripheral nerve injury results in severe loss of motor and sensory function in the affected limb. The gold standard for peripheral nerve repair is autologous nerve grafts, but their inherent drawbacks limit their use. Satisfactory clinical data are yet to be obtained using tissue engineered nerve grafts with neurotrophic factors introduced in these grafts for nerve repair. Therefore, peripheral nerve regeneration still remains a challenge for clinicians. Exosomes are secreted nanovesicles from the extracellular membrane. They are critical for communication within the cell and play a crucial role in the pathologic process of the peripheral nervous system. Recent research supports the role of exosomes in exhibiting neurotherapeutic effects through axonal growth, Schwann cell activation, and regulating inflammation. Indeed, the use of \"smart\" exosomes by reprogramming or manipulating the secretome contents and functions are rising as a therapeutic option for treating peripheral nerve defects. This review provides an overview on the promising role of exosomes in the process of peripheral nerve regeneration.</p>","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"6 2","pages":"189-196"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10310314/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10120222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and Characterization of Alginate/Collagen Composite Bioinks for Bone Tissue Engineering","authors":"T. Arahira, Kurumi Takimoto, M. Todo","doi":"10.26502/jbb.2642-91280080","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280080","url":null,"abstract":"Tissue engineering aims to construct artificial organs based on cells, growth factors, and scaffold materials. Scaffolds should promote cellular activity, provide sufficient nutrition, and remove waste products. In recent years, 3D bioprinters, which can suspend cells using bioink to fabricate 3D structures, are attracting considerable attention as a technology that enables uniform cell distribution. However, few studies have examined the mechanical properties of 3D scaffold by bioink. This study aims to develop a bioink with good mechanical properties that is easy to produce. As the result, compared with commercially available bioinks, the fabricated bioink is easy to prepare by mixing Alg-Na with a collagen solution and has superior mechanical properties. Our results indicate that some material properties, such as compressive modulus and surface structure, can be controlled by varying the ratio of collagen","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"154 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75735037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Study on Efficacy of Azithromycin and Minocycline in the Treatment of Acne Vulgaris","authors":"Farzana Afroz, R. Hasan, Rajib Shahriar","doi":"10.26502/jbb.2642-91280089","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280089","url":null,"abstract":"Background: Acne is a chronic inflammatory condition of the pilosebaceous units that is also a multifactorial skin lesion. Dermatologists treat acne vulgaris, one of the most prevalent skin conditions, which primarily affects teens but can occur at any age. Objective: The aim of the study to compare the efficacy of azithromycin and minocycline in the treatment of acne vulgaris. Methodology: This cross-sectional study was conducted at tertiary care hospital Dhaka District from June 2021 to July 2022. A total of 120 patients diagnosed with acne regardless of age and sex. Data collection was done after approval of protocol using a semi-structured questionnaire through face-to-face interview. Data were analyzed using a computer program SPSS 25.0 version. Result: The mean (±SD) of the patients. The mean (±SD) age of the total sample was 27.26(±4.97). In Azithromycin group it was 25.97(±3.86), In Minocycline group 23.87(±7.84). The minimum age of the total sample was 16 years, maximum age was 45. Among all the patients 65% were female. The reduction in the percentage, in the number of inflammatory lesions, non-inflammatory and total lesions of post six weeks was better with azithromycin group as compared to minocycline. Conclusion: Patients with acne who received azithromycin and minocycline separately in the current study, showed improvements in a number of criteria measuring the severity of the lesion. Affiliation: 1Associate Professor and Head, Department of Dermatology, Medical College for Women and Hospital, Dhaka, Bangladesh 2Associate Professor & Head, Department of Dermatology, US Bangla Medical College & Hospital, Narayanganj, Bangladesh 3Lieutenant Colonel, Department of Neurosurgery, Combined Military Hospital, Dhaka *Corresponding author: Dr. Farzana Afroz, Associate Professor and Head, Department of Skin and Venereal Diseases, Medical College for Women and Hospital, Dhaka, Bangladesh. Citation: Farzana Afroz, Rashidul Hasan, Rajib Shahriar. A Comparative Study on Efficacy of Azithromycin and Minocycline in the Treatment of Acne Vulgaris.Journal of Biotechnology and Biomedicine. 6 (2023): 265-269. Received: May 25, 2023 Accepted: June 05, 2023 Published: June 21, 2023","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"69 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76528825","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Emergency Contraception from Historical Myth to Modern Reality: A Historical Timeline and Updated Interpretation","authors":"Norman D Goldstuck","doi":"10.26502/jbb.2642-91280114","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280114","url":null,"abstract":"Introduction: Emergency contraception is the use of a birth control method after coitus has taken place and there is a fear that it may lead to a pregnancy. Historical attempts were more likely to be harmful rather than effective. Oral estrogens, progestins, anti- progesterone, and partial agonist/antagonists of progesterone have all been used with varying degrees of efficacy. Currently ethinyl estradiol/levonorgestrel combinations, levonorgestrel alone, ulipristal acetate, and mifepristone are the usual oral methods depending on availability. Copper carrying and more recently levonorgestrel releasing intrauterine devices have also been used successfully. The intrauterine devices appear to be more effective than the oral methods and are also regular contraceptive methods and in addition have therapeutic properties.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134889755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}