Journal of Biotechnology and Biomedicine最新文献_第2页

The Prevalence and Severity of Psychological Stress and Anxiety among Nurses of Tamale Teaching Hospital-Ghana 加纳塔梅尔教学医院护士心理压力和焦虑的发生率及严重程度

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280115

Nicholas Yakubu Danzima, Peter Mintir Amadu, Rejoice Enyonam Hoedoafia, Maliche Hawa, Vivian Kapio Abem, Samuel Paapa Kofi Paintsil

引用次数: 0

In vivo-like Culture of Monophagous Animal Organ using Dietary Components. 利用膳食成分进行单食性动物器官的类活体培养

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 Epub Date: 2023-02-10 DOI: 10.26502/jbb.2642-91280070

Norichika Ogata, Shogo Konishi, Takeshi Yokoyama

引用次数: 0

Taiyuanostachya: An Abominable Angiosperm from the Early Permian of China 太原石竹:一种来自中国早二叠世的可恶的被子植物

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280099

Xin Wang, Qiang Fu

{"title":"Taiyuanostachya: An Abominable Angiosperm from the Early Permian of China","authors":"Xin Wang, Qiang Fu","doi":"10.26502/jbb.2642-91280099","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280099","url":null,"abstract":"Although angiosperms are clearly and strictly defined by their enclosed seeds and enclosed ovules, how old angiosperms are remains controversial. To solve this problem, the only reliable way is digging fossils. The currently widely accepted earliest age for angiosperms is the Early Cretaceous, although this view is facing increasing challenges from pre-Cretaceous fossil evidence of angiosperms as well as molecular clock estimates. Here we report a Palaeozoic angiosperm, Taiyuanostachya gen. nov. This fossil plant has both enclosed ovules and enclosed seeds, features characteristic of angiosperms. Especially, enclosed ovules are idiosyncratic of angiosperms. The occurrences of both characters in Taiyuanostachya declare that angiosperms, the single most diversified plant group on the Earth, have occurred on the Earth in the Palaeozoic, and the origination of angiosperm appears to be much earlier than assumed previously. Although appearing astonishing, this conclusion is in line with the outcomes of molecular clock estimates done decades ago. The discovery of Taiyuanostachya gen. nov is an abominable challenge for botanists who believe groundlessly that angiosperms cannot exist before the Cretaceous.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"78 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85917616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

A flow Cytometry Gating Strategy for Profiling CD4+CD25+/highCD127-/low Regulatory T Cells in the Rheumatoid Arthritis Scenario 类风湿关节炎中CD4+CD25+/高cd127 -/低调节性T细胞的流式细胞术门控策略

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280083

Martínez Rosales Ricardo Ulises, Crespo Oliva Celia del Carmen, Chávez Valdés Sheila, Vazquez Arteaga Amalia, Coizeau Rodríguez Edelgis, Ávila Díaz Lismary, Freyre Corrales Giselle, Gonzalez Formental Hany Lianet, Chacón Quintero Yahima, Lemos Perez Gilda

引用次数: 0

Multi-Omics Analyses Revealed Transcriptional Regulators associated with Immune Checkpoint Inhibitor Treatment in Advanced Bladder Cancer 多组学分析揭示了与免疫检查点抑制剂治疗晚期膀胱癌相关的转录调节因子

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280071

F. Xu, Zuheng Wang, D. Fu, Xiuquan Shi, Jie Huang, Yuhao Chen, Jianping Da, Tinglin Zhang, Jingping Ge, Xiao-feng Xu, Wen Cheng

{"title":"Multi-Omics Analyses Revealed Transcriptional Regulators associated with Immune Checkpoint Inhibitor Treatment in Advanced Bladder Cancer","authors":"F. Xu, Zuheng Wang, D. Fu, Xiuquan Shi, Jie Huang, Yuhao Chen, Jianping Da, Tinglin Zhang, Jingping Ge, Xiao-feng Xu, Wen Cheng","doi":"10.26502/jbb.2642-91280071","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280071","url":null,"abstract":". Abstract Background: Urothelial Bladder Cancer (UBC) is one of the most lethal cancers worldwide, the 5-year survival rate remains poor with platinum-based chemotherapy regimens as the standard of cancer treatment protocol. Recent FDA approval of a programmed death ligand-1 (PD-L1) inhibitor, atezolizumab, in advanced UBC patients is changing the therapeutic landscape. Although the response to anti-PD-L1 is correlated to PD-L1 expression and tumor mutation burden, the molecule determinants of responsiveness or non-responsiveness to Immune Checkpoint Inhibitor (ICI) is largely unknown. Methods: R package maftools was used for genomic characterization and differential mutational analysis. EdgeR and DysRegSig algorithm were used for differential gene expression and dysregulator analysis. ConcensusTME algorithm was used for deconvolution of cell types within tumor microenvironment from bulk RNAseq data. Result: A published immunotherapy cohort with whole exome sequencing, RNAseq and clinic outcome data for 29 metastatic urothelial cancer patients was used, paralleled with The Cancer Genome Altas (TCGA) Bladder Cancer cohort,","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88082368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Metabolic Shifting Probiotic in Type 2 Diabetes Mellitus Management: Randomized Clinical Trial 代谢转移益生菌治疗2型糖尿病:随机临床试验

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280090

G. García, Josanne Soto, L. Rodríguez, M. Nuez, N. Dominguez, Emilio F. Buchaca, D. Martínez, Rolando J Gómez, Y. Ávila, Martha R. Carlin, Raúl J. Cano

{"title":"Metabolic Shifting Probiotic in Type 2 Diabetes Mellitus Management: Randomized Clinical Trial","authors":"G. García, Josanne Soto, L. Rodríguez, M. Nuez, N. Dominguez, Emilio F. Buchaca, D. Martínez, Rolando J Gómez, Y. Ávila, Martha R. Carlin, Raúl J. Cano","doi":"10.26502/jbb.2642-91280090","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280090","url":null,"abstract":"Objective: The objective of this study is to assess the efficacy of BiotiQuest™ Sugar Shift, a probiotic formulated to transform monosaccharides, rehabilitated human gut microbiota, stabilize HbA1c and blood glucose, improve insulin resistance and reduce inflammation Methods: A double-blind, placebo-controlled study was carried out over 12 weeks with 64 Cubans, aged 30 to 65 with Type 2 Diabetes Mellitus diagnosed, 18 of whom being treated with insulin. Participants were randomly assigned to take either two capsules of the probiotic supplement or of placebo. Clinical measures were evaluated at 28-day intervals, included fasting and post-prandial glucose, HbA1c, lipid panel, insulin, creatinine, and serum lipopolysaccharide levels were assessed. Results: The treated group demonstrated a stabilization in their fasting blood glucose and postprandial glucose levels in treated group respect to the Placebo cohort. The HbA1c levels did not show significant changes in the treated group. The insulin levels decreased significantly in the treated group by day 84 compared to day 1 and to day 84 of the Placebo cohort (p=0.024 and p=0.015, respectively). Serum LPS levels also decreased significantly in the treated group (p=0.001). Conclusions: BiotiQuest™ Sugar Shift is suitable as adjunct therapy for the control of T2D. However, the 12-week trial period was not sufficient to detect significant reductions in all clinical parameters measured and a longer study is recommended.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83877294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

SARS-Cov-2 Spike RBD and Nucleocapsid Encoding DNA Vaccine Elicits T cell and Neutralising Antibody Responses that Cross React with Variants SARS-Cov-2刺突RBD和核衣壳编码DNA疫苗引发T细胞和中和抗体反应，与变体交叉反应

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280085

Brentville Va, Vankemmelbeke M, Metheringham Rl, Symonds P

引用次数: 1

Gating Of the Channel Pore of Ionotropic Glutamate Receptors with Bacterial Substrate Binding Proteins: Functional Coupling of the Ectoine Binding Protein Ehub to Glur0 细菌底物结合蛋白对离子型谷氨酸受体通道孔的门控:外托因结合蛋白Ehub与Glur0的功能偶联

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280117

Max Bernhard, Bodo Laube

{"title":"Gating Of the Channel Pore of Ionotropic Glutamate Receptors with Bacterial Substrate Binding Proteins: Functional Coupling of the Ectoine Binding Protein Ehub to Glur0","authors":"Max Bernhard, Bodo Laube","doi":"10.26502/jbb.2642-91280117","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280117","url":null,"abstract":"Mammalian neuronal tetrameric ionotropic glutamate receptors (iGluRs) are thought to have originally arisen from the fusion of a bacterial substrate binding protein (SBP) with an inverted potassium channel. This hypothesis is based on structural and sequential similarities between the ligand binding and channel domains of iGluR subunits with SBPs and potassium channels. Ligand binding occurs at the interface between two lobed domains in both ligand binding domains (LBDs) and leads to closure of the shell-like structure, which is considered to be a key element in the transition from ligand recognition to ion channel gating in iGluRs. Here we report the functional coupling of the ectoine-binding protein EhuB to the channel pore of the GluR0 receptor. Fusion of an unmodified EhuB-binding protein to the transmembrane domain of GluR0 did not result in activation of the channel pore. Only by stabilizing the inserted EhuB-binding domain with a dimerization interface the resulting chimera was activated by ectoine, resembling the activation properties of other iGluRs. These results demonstrate the functional compatibility of SBPs to the gate of the channel pore of an iGluR and highlight the role of LBD dimerization in the functional evolution of iGluRs. Based on the high specificity and affinity of SBPs for an incredible variety of substrates, our results demonstrate the competence of SBP/ion channel chimeras for the development of new Biosensors for specific recognition of analytes by functionally linking a bacterial binding protein to the channel pore of an iGluR.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134889763","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic Polymorphism in Porphyria: Resolution of A Case of Tetraplegia by Administration of Hemin During a Porphyric Crisis and Subsequent Therapeutic Procedure with the use of Givosiran 卟啉症的遗传多态性:在卟啉危机期间给予Hemin和随后使用Givosiran的治疗过程中解决一个四肢瘫痪病例

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280095

Guida Cc, Nardella M, Perez Ys A, Recchia A, Tonti Mp, Savino M, Crisetti A, Napolitano F, Ferrara G, Aucella F, Manuali A

{"title":"Genetic Polymorphism in Porphyria: Resolution of A Case of Tetraplegia by Administration of Hemin During a Porphyric Crisis and Subsequent Therapeutic Procedure with the use of Givosiran","authors":"Guida Cc, Nardella M, Perez Ys A, Recchia A, Tonti Mp, Savino M, Crisetti A, Napolitano F, Ferrara G, Aucella F, Manuali A","doi":"10.26502/jbb.2642-91280095","DOIUrl":"https://doi.org/10.26502/jbb.2642-91280095","url":null,"abstract":"Porphyria is a very rare genetic pathology due to congenital defects of heme metabolism with deficiency of one of the enzymes that determine specific forms of porphyria. Acute hepatic porphyrias (AEP) includes four different clinical forms: a) from ALA D deficiency, b) acute intermittent porphyria, c) hereditary coproporphyria and d) variegate porphyria. The forms of porphyria are characterized by elevated levels of delta aminolevulinic acid (ALA) and porphobilinogen (PBG). Acute intermittent porphyria (AIP) is caused by a genetic mutation of hydroxymethylbilane synthetase (HMBS), which in a good percentage (about 20% of cases) does not show a positivity for hepatic porphyria on biomolecular examination, so that diagnosis with relative therapy is determined by the clinical condition. Our study describes a 54-year-old woman who, due to a porphyria crisis, presented serious symptoms with progressive aggravation that lead her to be hospitalized in an intensive care unit where she was intubated due to tetraplegia and severe respiratory failure. Treatment with human emin first and Givosiran subsequently allowed progressive amelioration of the clinical conditions.","PeriodicalId":15066,"journal":{"name":"Journal of Biotechnology and Biomedicine","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78524132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crambe Abyssinica Oil as Raw Material for Renewable Fuel Production-Review 深海石油作为可再生燃料生产原料的研究进展

Journal of Biotechnology and Biomedicine Pub Date : 2023-01-01 DOI: 10.26502/jbb.2642-91280094

Francisco Aléxis Dntas Maia, Amanda Duarte Godim, Anne Gabriella Dias Santos, Márcio Cleivo de Morais Souza, Vasco de Lima Pinto, Aruzza Mabel de Morais Araújo

引用次数: 0