A flow Cytometry Gating Strategy for Profiling CD4+CD25+/highCD127-/low Regulatory T Cells in the Rheumatoid Arthritis Scenario

Abstract

Regulatory T cells (Tregs) has been consider as a potential biomarker in rheumatologic clinical trials as this cell population has been shown to be involved in the autoimmune diseases outcome. Human Tregs are defined as CD4 + CD25 +/high CD127 -/low cells; however its accurate identification by flow cytometry has been argued, especially as a result of different gating strategies. A flow cytometry gating strategy was settled to measure human Tregs from PBMC isolated via density gradient using CPT tubes. With this method we performed a standardized procedure for Treg profiling, useful for immunomonitoring in clinicals assessment