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Nucleolin Transports Hsp72 to the Plasma Membrane Preparatory to itsRelease into the Microenvironment 核蛋白将Hsp72转运到质膜，准备释放到微环境中
Journal of Cell Science and Therapy Pub Date : 2016-10-28 DOI: 10.4172/2157-7013.1000254
Appukuttan Nr Pradeep, Alexz, er Asea, P. Kaur
{"title":"Nucleolin Transports Hsp72 to the Plasma Membrane Preparatory to itsRelease into the Microenvironment","authors":"Appukuttan Nr Pradeep, Alexz, er Asea, P. Kaur","doi":"10.4172/2157-7013.1000254","DOIUrl":"https://doi.org/10.4172/2157-7013.1000254","url":null,"abstract":"Purpose: Our previous studies demonstrated that thermal stress induces the release of Hsp72 from cells by a mechanism independent of the classical protein transport pathway. However, the exact mechanism by which Hsp72, a leaderless protein, gains access to the extracellular milieu remains unknown. \u0000Materials and methods: This study is designed to determine the mechanism by which intracellular Hsp72 trafficking and release occurs. The data presented in this study suggests intracellular Hsp72 trafficking using Western blotting and Flow Cytometry. Also, unknown membrane bound proteins were identified doing in-gel digestion and LC-MS/MS. \u0000Results: We demonstrate that within 60 minutes after first exposure of cells to heat shock treatment, plasma membrane bound Hsp72 is shed and redistributed into cytosolic compartments. Inhibition of active cell transport by pre-treatment of cells with Cytochalasin B completely abrogated Hsp72 redistribution from the plasma membrane into the cytosol. Cross-linking of plasma membrane bound proteins with Hsp72 followed by Western blot analysis and LC-MS/MS analysis revealed at least seven interacting partners with Hsp72, including nucleolin, Hsp90, gp96, CAP2, TLR2, 4 and 7. Transfection of cells with nucleolin-siRNA completely inhibited baseline and heat shockinduced Hsp72 release. \u0000Conclusions: Taken together, this study for the first time demonstrates that the plasma membrane acts as a reservoir for Hsp72 and suggests that nucleolin plays an important role in Hsp72 trafficking and release.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"65 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125958850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Stem Cells and Cancer 干细胞与癌症
Journal of Cell Science and Therapy Pub Date : 2016-10-28 DOI: 10.4172/2157-7013.1000255
A. Ali
{"title":"Stem Cells and Cancer","authors":"A. Ali","doi":"10.4172/2157-7013.1000255","DOIUrl":"https://doi.org/10.4172/2157-7013.1000255","url":null,"abstract":"Stem cells are undifferentiated cells that can differentiate into specialized cell and can divide to produce more stem cells. The concept of cancer stem cells has been discussed in the scientific literature since the 19th century. Circumstantial evidence suggests that most tumors are heterogeneous and contain a small population of cancer stem cells that exhibit distinctive self-renewal, proliferation and differentiation capabilities, which are believed to play a crucial role in tumor progression, drug resistance, recurrence and metastasis in multiple malignancies. Unlike normal adult stem cells that remain constant in number, cancer stem cells can increase in number as tumors grow, and give rise to progeny that can be both locally invasive and colonize distant sites-the two hallmarks of malignancy. Rapid advances in the cancer stem cell feld have provided cause for optimism for the development of more reliable cancer therapies in the future. Strategies aimed at effcient targeting of cancer stem cells are becoming important for monitoring the progress of cancer therapy and for evaluating new therapeutic approaches.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"172 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131946169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Cardiac Output and Renal Function: An Association 心输出量与肾功能:一种关联
Journal of Cell Science and Therapy Pub Date : 2016-09-29 DOI: 10.4172/2157-7013.1000252
Ibad Ghori, I. Ahmed, Fareeha Bukhari, Hassaan Tohid
{"title":"Cardiac Output and Renal Function: An Association","authors":"Ibad Ghori, I. Ahmed, Fareeha Bukhari, Hassaan Tohid","doi":"10.4172/2157-7013.1000252","DOIUrl":"https://doi.org/10.4172/2157-7013.1000252","url":null,"abstract":"In this article, we discussed a clinical problem known as cardio renal syndrome (CRS). In CRS, both, the affected cardiovascular system, either low output or high output failure leads to the renal dysfunction or vice versa. As already known, the heart failure is the leading cause of readmission in the US and worldwide. The patients suffering with cardiac failure need extra care, as they may develop various complications. Cardiac output is affected in heart failure and does have an impact on the renal function. High cardiac output and low cardiac output, both can lead to renal dysfunction. In conclusion, we will also highlight some information about the prevention of the renal damage associated with the CVS. However, future research is recommended to understand more about the exact pathophysiology involved in this association.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114888871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Dissociative Identity Disorder: A Pathophysiological Phenomenon 分离性身份障碍:一种病理生理现象
Journal of Cell Science and Therapy Pub Date : 2016-09-29 DOI: 10.4172/2157-7013.1000251
A. Ashraf, R. Krishnan, Eden Wudneh, Ajita Acharya, Hassaan Tohid
{"title":"Dissociative Identity Disorder: A Pathophysiological Phenomenon","authors":"A. Ashraf, R. Krishnan, Eden Wudneh, Ajita Acharya, Hassaan Tohid","doi":"10.4172/2157-7013.1000251","DOIUrl":"https://doi.org/10.4172/2157-7013.1000251","url":null,"abstract":"Various hollywood movies have shown a character who switches his personality, one personality does not remember the other at all. In the psychiatric world such a condition is called dissociative identity disorder (DID). The current study shows a collection of some studies showing the pathologic nature of the phenomenon. In this review, we have shown some studies including MRI studies, that demonstrated that the limbic system of DID patients decreases in size (especially hippocampus and amygdala). Moreover, a reduction in the functioning of orbitofrontal cortex is also observed with a reduced cerebral blood in DID patients. In the end, we recommend more studies in the future, to gain more insight about the pathophysiology of this mysterious condition, that will be helpful to treat and manage the patients with DID successfully in the near future.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-09-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129740746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Charles Bonnet Syndrome: A Case Study 邦纳症候群:个案研究
Journal of Cell Science and Therapy Pub Date : 2016-08-31 DOI: 10.4172/2157-7013.1000250
M. Siddiqui, Khan Tj, B. Smith, S. Travis, Hassaan Tohid
{"title":"Charles Bonnet Syndrome: A Case Study","authors":"M. Siddiqui, Khan Tj, B. Smith, S. Travis, Hassaan Tohid","doi":"10.4172/2157-7013.1000250","DOIUrl":"https://doi.org/10.4172/2157-7013.1000250","url":null,"abstract":"We present a rare case of visual release hallucinations also referred to as Charles bonnet syndrome. The disorder is rare and no officially recognized mode of treatment has been developed. However, we hope that as the time goes by, and more cases of CBS are found. It will be possible for the scientists and the clinicians to find a permanent and a better cure for this rare mystery.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-08-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114452964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The Lack of Trained Child Psychiatrists Leading to Underreported Casesof ADHD in Pakistan 缺乏训练有素的儿童精神科医生导致巴基斯坦少报ADHD病例
Journal of Cell Science and Therapy Pub Date : 2016-08-26 DOI: 10.4172/2157-7013.1000E125
A. Ashraf, Hassaan Tohid
{"title":"The Lack of Trained Child Psychiatrists Leading to Underreported Casesof ADHD in Pakistan","authors":"A. Ashraf, Hassaan Tohid","doi":"10.4172/2157-7013.1000E125","DOIUrl":"https://doi.org/10.4172/2157-7013.1000E125","url":null,"abstract":"The dearth of child psychiatrists in Pakistan is becoming one of the major causes of many cases of attention deficit hyperactivity disorder (ADHD) remain undiagnosed. Some important measures must be taken at the private and the governmental levels, so that the ADHD cases do not remain diagnosed in the future.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"70 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126491132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Chromothripsis in Lipoblastoma 脂肪母细胞瘤的染色体脱落
Journal of Cell Science and Therapy Pub Date : 2016-08-26 DOI: 10.4172/2157-7013.1000249
F. Pederiva, C. Vanessa, ilera, L. Cleva, V. Pecile
{"title":"Chromothripsis in Lipoblastoma","authors":"F. Pederiva, C. Vanessa, ilera, L. Cleva, V. Pecile","doi":"10.4172/2157-7013.1000249","DOIUrl":"https://doi.org/10.4172/2157-7013.1000249","url":null,"abstract":"Lipoblastoma is a benign tumor that often has rearrangements of the 8q11-13 region targeting the PLAG1 gene, which is involved in the process of tumourigenesis. We described a signature of chromotripsis in a thigh lipoblastoma, as first report of chromotripsis in a benign tumor.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"384 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131857436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Human Cementoblastoma- Derived Cells Express Cartilage- LikePhenotype In vitro and In vivo and Induce Bone Formation 人成骨水泥细胞瘤来源的细胞在体外和体内表达软骨样表型并诱导骨形成
Journal of Cell Science and Therapy Pub Date : 2016-08-25 DOI: 10.4172/2157-7013.1000248
L. Hoz-Rodriguez, Ana L Garcia-Hern, Ez, Enrique Romo-Arévalo, Fabiola Salgado-Chavarría, G. Montoya-Ayala, M. Zeichner‐David, Rodrigo Correa-Prado, Sonia Lopez-Letayf, H. Arzate
{"title":"Human Cementoblastoma- Derived Cells Express Cartilage- LikePhenotype In vitro and In vivo and Induce Bone Formation","authors":"L. Hoz-Rodriguez, Ana L Garcia-Hern, Ez, Enrique Romo-Arévalo, Fabiola Salgado-Chavarría, G. Montoya-Ayala, M. Zeichner‐David, Rodrigo Correa-Prado, Sonia Lopez-Letayf, H. Arzate","doi":"10.4172/2157-7013.1000248","DOIUrl":"https://doi.org/10.4172/2157-7013.1000248","url":null,"abstract":"The molecular mechanisms that regulate proliferation and differentiation of cementoblasts, have not been elucidated to date. In this paper, it is shown that human cementoblastoma-derived cells (HCDC) express greater levels of cartilage markers such as type II and X collagens, aggrecan (ACAN) and SRY-box 9 (SOX9) stem cell markers; MCAM (melanoma cell adhesion molecule; synonym: CD146) and STRO-1 than human gingival fibroblasts (HGF). Our in vivo studies demonstrate that HCDC induce bone formation through endochondral ossification as observed 14 days after HCDC were implanted in rat critical-size calvarial defects. At 30 and 60 days post-implantation, the defects treated with HCDC were filled with 70 ± 1.6 and 91 ± 1.3% of newly formed bone. To confirm the identity of this tissue, we analyzed the newly formed bone using histomorphology and immunostaining. The results showed the expression of bone sialoprotein (BSP) and osteocalcin (OCN). Immmunostaining of human periodontal structures showed that cementoblasts and periodontal ligament cells express cementum protein 1 (CEMP1), cartilage markers, type II and X collagens and CD146 which has been identified as a marker for chondroprogenitor cells. Altogether these results indicate that HCDC has the capacity of multilineage differentiation and induces regeneration of mineralized tissues other than cementum.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122788147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pathogenicity of Entamoeba Species Depends on Cell Line Conversion,Genome Reprogramming and Epigenetic Gene Regulation 内阿米巴原虫的致病性取决于细胞系转化、基因组重编程和表观遗传基因调控
Journal of Cell Science and Therapy Pub Date : 2016-06-29 DOI: 10.4172/2157-7013.1000245
V. Niculescu
{"title":"Pathogenicity of Entamoeba Species Depends on Cell Line Conversion,Genome Reprogramming and Epigenetic Gene Regulation","authors":"V. Niculescu","doi":"10.4172/2157-7013.1000245","DOIUrl":"https://doi.org/10.4172/2157-7013.1000245","url":null,"abstract":"The protist life cycle is certainly much more than a simple sequence of trophic and non-trophic stages (cysts), and trophic cells (trophozoites) do not divide categorically into two identical daughter cells. The pathogenic amoebae Entamoeba histolytica and Entamoeba invadens exhibit complex life cycles including stem cells and cell lines following various biological tasks such as virulence and encystment. Intrinsic and extrinsic molecular mechanisms controlled by environmental cues, develop in both species a PST stem cell lineage that consists of primary, secondary and tertiary self-renewing cell lines. Oxygen gradients controlled by the host intestine and bacteria initiate the stem cell lineage machinery and are responsible for cell line conversions. Entamoeba dispar has a similar PST stem cell lineage despite being less pathogenic. These three amoebic species begin their life cycle with a primary multipotent stem cell line (p-SRL) that starts from metacystic amoebulae. The p-SRL line converts to progenitor cell lines depending on the environmental oxygen content. Progenitor cell lines are of reduced potency. The secondary s-SRL line produce mitotic arrested MAS cells (cyst precursor cells) committed for terminal differentiation; they continue development entering the endopolyploid cell cycle, a developmental cycle opponent to mitosis and form cysts. The tertiary t-SRL line does not form cysts. It produces mitotic quiescent MAT cells that enter a state of G0 and mature to invasive cells of variable genotypic virulence. MAT cells reentering mitotic cycle form new t-SRL variants. In hypoxic conditions the t-SRL line changes to symmetric cell fate","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115111860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
A Rat Tail Model for Osseointegration Studies and New Bone Formation Follow-up 用于骨整合研究和新骨形成随访的大鼠尾巴模型
Journal of Cell Science and Therapy Pub Date : 2016-06-27 DOI: 10.4172/2157-7013.1000244
Matthieu Renaud, or Farkasdi, A. Desoutter, G. Varga, F. Cuisinier, P. Bousquet
{"title":"A Rat Tail Model for Osseointegration Studies and New Bone Formation Follow-up","authors":"Matthieu Renaud, or Farkasdi, A. Desoutter, G. Varga, F. Cuisinier, P. Bousquet","doi":"10.4172/2157-7013.1000244","DOIUrl":"https://doi.org/10.4172/2157-7013.1000244","url":null,"abstract":"Animal studies are necessary to precede clinical studies. Recently the rat tail model has been proposed by us as a model for studying bone regeneration with easily surgical approach, effective control of post-operative pain and a decrease of animal number. The present study aimed to widen the rat tail model indication to implant osseointegration. Special titanium implants were inserted through tail vertebrae. A good primary stability was observed three month after implant placement. X-ray microtomography (Micro-CT) and histology were used to visualize bone formation and to calculate bone implant contact. Micro-CT showed osseointegrated implants in caudal vertebra. This illustrates the possibility to obtain bone implant-contact by micro-CT measurements. The results suggest that the rat caudal vertebrae may serve as a good preclinical model for studying implant osseointegration with the possibility of multiple testing within the same experimental animal and the potential to decrease number of experimental animals.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131114925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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