Journal of Cell Science and Therapy最新文献_第4页

A Review on Pyridazinone Compounds ABT-963 as Selective CyclooxygenaseInhibitor 吡嗪酮类化合物ABT-963作为选择性环氧化酶抑制剂的研究进展

Journal of Cell Science and Therapy Pub Date : 2016-05-30 DOI: 10.4172/2157-7013.1000243

M. Asif

引用次数: 2

Application of Hypoxia to Establish Epithelial-Mesenchymal Transition Models inRenal Cell Carcinoma Cell Lines 缺氧在建立肾细胞癌上皮-间质转化模型中的应用

Journal of Cell Science and Therapy Pub Date : 2016-05-30 DOI: 10.4172/2157-7013.1000242

B. Hong, Changhua Zhou, Xin Du, Siqi Chen, Xiaohu Deng, Shayiremu Duoerkun, Qing Li, Yong Yang, K. Gong, Ning Zhang

{"title":"Application of Hypoxia to Establish Epithelial-Mesenchymal Transition Models inRenal Cell Carcinoma Cell Lines","authors":"B. Hong, Changhua Zhou, Xin Du, Siqi Chen, Xiaohu Deng, Shayiremu Duoerkun, Qing Li, Yong Yang, K. Gong, Ning Zhang","doi":"10.4172/2157-7013.1000242","DOIUrl":"https://doi.org/10.4172/2157-7013.1000242","url":null,"abstract":"Purpose: To establish epithelial-mesenchymal transition (EMT) models in renal cell carcinoma cell lines by CoCl2-induced hypoxia. \u0000Materials and methods: The renal cell carcinoma cell lines A498 and 786-O were used in the experiment and CoCl2 was used to simulate hypoxia. The cancer cells were cultured with different concentrations of CoCl2. Morphology and cyto-activity changes were detected to obtain the optimal concentration of CoCl2 for simulating hypoxia. After CoCl2 treatment, the cells were subjected to Western blot analysis to test the expression of HIF-1α and the changes of EMT-related molecules (E-cadherin, fibronectin). \u0000Results: Cell conjunctions of CoCl2-treated groups were loose and scattered compared to the control. The effect of CoCl2 on A498 cell viability was not distinct at a low dosage, but when the concentration of CoCl2 reached 250 mM, cell activity gradually declined. In contrast, CoCl2 induced 786-O cell proliferation in the range of 50 mM- 200 mM, but it inhibited cell growth at dosages higher than 200 mM. The expression of E-cadherin was significantly down-regulated, and fibronectin was up-regulated in both A498 and 786-O cell lines under CoCl2-simulated hypoxia in comparison with normoxic conditions (P<0.01). \u0000Conclusions: EMT models of the renal cell carcinoma cell lines were successfully established by CoCl2-induced hypoxia. The models will help us further study the mechanisms of EMT and investigate novel therapeutic targets to inhibit tumor invasion and metastasis.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"18 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125707761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Melatonin: Antiproliferative Actions and Protection of Normal Tissue andEnhancement of Radiosensitivity of Breast Cancer Cells 褪黑素:对正常组织的抗增殖作用和保护作用，以及增强乳腺癌细胞的放射敏感性

Journal of Cell Science and Therapy Pub Date : 2016-04-25 DOI: 10.4172/2157-7013.1000241

C. Campa, C. Alonso-González, Alicia González, Alicia González-González, Javier Menéndez-Menéndez, S. Cos

引用次数: 8

Evaluation of the Role of Adipose-Derived Stem Cells in the Healing ofIndomethacin-Induced Gastric Ulceration in Rats 脂肪源性干细胞在吲哚美辛诱导大鼠胃溃疡愈合中的作用评价

Journal of Cell Science and Therapy Pub Date : 2016-03-29 DOI: 10.4172/2157-7013.1000246

Sally M Abd-Elmenm, Sahar M Greish, M. Atwa, M. Fathelbab

{"title":"Evaluation of the Role of Adipose-Derived Stem Cells in the Healing ofIndomethacin-Induced Gastric Ulceration in Rats","authors":"Sally M Abd-Elmenm, Sahar M Greish, M. Atwa, M. Fathelbab","doi":"10.4172/2157-7013.1000246","DOIUrl":"https://doi.org/10.4172/2157-7013.1000246","url":null,"abstract":"Purpose: To study the effect adipose derived stem cells (ADSCs) in a rat model of gastric ulceration. \u0000Methods: 72 albino rats divided into 3 groups: Group N (Negative control), Group D (positive control); and Group T (ADSCs treated group). Six animals of each group were sacrificed 1, 3, 4 and 5 days after ulcer induction. Each stomach was extracted for macroscopical and histopathological assessment. Vascular endothelial growth factor (VEGF), Prostaglandin E2 (PGE2) and polymerase chain reaction (PCR) for human alu sequences in gastric tissue homogenate were done. \u0000Results: ADSCs transplantation improved the histopathology of the gastric tissue in stem cell treated group. Also the result of ulcer index was significantly decreased in stem cell treated group at day 3, 4 and 5 of the study comparing with the indomethacin challenged group. Regarding ELISA results, ADSCs restored the levels of PGE2 to the normal levels and increased the VEGF levels significantly to above normal levels. PCR revealed that stem cells successfully engulfed into the gastric wall by the third day and continued to persist until the 5th day. \u0000Conclusion: Homing of stem cells, release of growth factors that induce angiogenesis like VEGF and other substances as PGE2 may be the underlying mechanisms of the improved gastric ulceration healing.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122689221","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Mechanisms of cancer progression: Roles of osteopontin variants 癌症进展机制:骨桥蛋白变异的作用

Journal of Cell Science and Therapy Pub Date : 2016-03-29 DOI: 10.4172/2157-7013.C1.034

G. Weber

引用次数: 0

Macroscopic and Microscopic Study of Allium sativum (Garlic) Oil Effects onPost-Operative Intra-Abdominal Adhesion in Diabetic and Non-diabetic Male Rats 大蒜油对糖尿病和非糖尿病雄性大鼠术后腹腔粘连影响的宏观和微观研究

Journal of Cell Science and Therapy Pub Date : 2016-03-16 DOI: 10.4172/2157-7013.1000240

Saleh N Ali, M. Badawi, Rania A. Galhom, F. Badr

{"title":"Macroscopic and Microscopic Study of Allium sativum (Garlic) Oil Effects onPost-Operative Intra-Abdominal Adhesion in Diabetic and Non-diabetic Male Rats","authors":"Saleh N Ali, M. Badawi, Rania A. Galhom, F. Badr","doi":"10.4172/2157-7013.1000240","DOIUrl":"https://doi.org/10.4172/2157-7013.1000240","url":null,"abstract":"Backgrounds: Development of peritoneal adhesions has been studied extensively, but to date, no definitive strategy has been implemented to prevent their formation. In the present study, garlic oil has been investigated as a possible option. \u0000Aim: To prevent post-operative intra-abdominal adhesion in both diabetic and non-diabetic rats by using garlic oil. \u0000Methodology: An experimental study including sixty adult male albino rats weighing 200-250 gm. were divided to six equal groups. Diabetes was induced in rats of three groups using a single dose of 120 mg alloxan/kg/BW. All rats underwent laparotomy at which cecal wall abrasion and abdominal wall injuries were induced. Group A (negative control) underwent no procedures; Groups B (positive control) underwent diabetic induction. Group C underwent cecal abrasion procedure and received saline 5 ml/kg/BW intraperitoneally. Group D underwent abrasion and received garlic oil 5 ml/kg/BW intraperitoneally. Group E underwent abrasion, diabetes induction and received saline 5 ml/kg/ BW. Group F underwent abrasion, diabetes induction and received garlic oil 5 ml/kg/BW. All rats were sacrificed on day 14 postoperative, and the severity of adhesions was evaluated using histo-pathological fibrosis parameters and immune-histochemical staining to identify the fibro vascular tissue nature. \u0000Results: Significant differences were found between the groups regarding macroscopic adhesion scores, inflammation, fibrosis and neo-vascularization (p<0.001, p<0.001, p<0.001, p<0.005, respectively). Macroscopic and histo-pathologic adhesion scores were the lowest in the garlic oil-treated diabetic and non-diabetic groups. \u0000Conclusion: The results of our study revealed the effectiveness of garlic oil in reducing postoperative adhesions in both non-diabetic and diabetic rats.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129969591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Reappraisal of the Significance of Inducible Nitric Oxide Synthase inColorectal Cancer 诱导型一氧化氮合酶在结直肠癌中的意义

Journal of Cell Science and Therapy Pub Date : 2016-03-11 DOI: 10.4172/2157-7013.1000239

Chung-Ta Lee, Peiran Su, P. Lin, H. Tsai, C. Lam, B. Lin, Shao‐Chieh Lin, N. Chow, J. Lee

{"title":"Reappraisal of the Significance of Inducible Nitric Oxide Synthase inColorectal Cancer","authors":"Chung-Ta Lee, Peiran Su, P. Lin, H. Tsai, C. Lam, B. Lin, Shao‐Chieh Lin, N. Chow, J. Lee","doi":"10.4172/2157-7013.1000239","DOIUrl":"https://doi.org/10.4172/2157-7013.1000239","url":null,"abstract":"Backgrounds: Inducible nitric oxide synthase (iNOS), which produces high levels of nitric oxide (NO), is overexpressed in activated macrophages and some cancer cells. Although iNOS was thought to be involved in promoting colorectal cancer, contradictory reports supporting its tumoricidal effect exist. Methods: We first examined the iNOS enzyme activity in colorectal cancer tissue and immunohistochemical expression of iNOS in cancer cells and tumor-infiltrating macrophages. Then, association of iNOS activity or its protein expression was analyzed in relation to various clinicopathological covariates. \u0000Results: Four groups of patients were classified based on their iNOS expression status. Univariate and multivariate analyses showed that group 1 patients (low iNOS in both types of cells) and group 4 patients (high iNOS in both types of cells) had a shorter disease-free survival. Patients with extremely high or low iNOS enzyme activity tended to have a lower disease-free survival rate (p = 0.059). \u0000Conclusion: Macrophage/stroma-derived NO negatively regulates colorectal cancer development when cancer cells express low levels of iNOS, but might synergistically contribute to tumor progression in the presence of high levels of cancer-cell derived NO. The dual effects of NO should be considered in the design of anti-iNOS/NO therapy for colorectal cancer patients.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2016-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116007964","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Regenerative Surgery in the Management of the Leg Ulcers 再生手术治疗腿部溃疡

Journal of Cell Science and Therapy Pub Date : 2016-03-09 DOI: 10.4172/2157-7013.1000238

L. Trovato, G. Failla, S. Serantoni, F. Palumbo

引用次数: 24

Inflammatory Fibroid Polyps of Large Bowel with PDGFRA Mutation 大肠炎性肌瘤息肉伴PDGFRA突变

Journal of Cell Science and Therapy Pub Date : 2016-02-18 DOI: 10.4172/2157-7013.1000234

Kanazu Ariyasu, R. Kitazawa, Ryuma Haraguchi, Y. Ueda, Y. Kawanami, Yukiko Nishi, Y. Kameoka, Y. Mizuno, S. Kitazawa

引用次数: 2

The Asterias Rubens Complement System: Comparisons with LowerVertebrates 星形补体系统:与低等脊椎动物的比较

Journal of Cell Science and Therapy Pub Date : 2016-02-15 DOI: 10.4172/2157-7013.1000236

M. Leclerc, Nicolas Kresdorn

引用次数: 2