Journal of Cell Science and Therapy最新文献

Schwann Cell Supplementation in Neurosurgical Procedures after Neurotrauma 神经外伤后补充雪旺细胞在神经外科手术中的应用

Journal of Cell Science and Therapy Pub Date : 2018-03-16 DOI: 10.4172/2157-7013.1000281

Santiago R. Unda

引用次数: 0

Holistic Healing- A Current Need of Holistic Medicine 整体治疗-整体医学的当前需求

Journal of Cell Science and Therapy Pub Date : 2018-02-19 DOI: 10.4172/2157-7013.1000280

Shrihari Tg

引用次数: 0

Analysis of Pluripotent Cell in Post-circumcised Preputial Skin using Cold Transport Methods (Ice and Dry Ice) with Oct-4 Expression 冷运输法(冰和干冰)对包皮环切术后多能细胞Oct-4表达的分析

Journal of Cell Science and Therapy Pub Date : 2017-12-26 DOI: 10.4172/2157-7013.1000279

A. Clarissa, R. Antarianto

{"title":"Analysis of Pluripotent Cell in Post-circumcised Preputial Skin using Cold Transport Methods (Ice and Dry Ice) with Oct-4 Expression","authors":"A. Clarissa, R. Antarianto","doi":"10.4172/2157-7013.1000279","DOIUrl":"https://doi.org/10.4172/2157-7013.1000279","url":null,"abstract":"The discovery of pluripotent cells in preputial skin, a previously discarded tissue, means prepuce can become a new source of stem cell banking which can be beneficial for the donor and his family. In transporting preputial skin to the biobank, we wanted to see simpler and inexpensive cold transport methods using dry ice and ice rather than liquid nitrogen to preserve the stem cells. The preputial skins were obtained from mass circumcision with informed consents and transported into the laboratory with dry ice or ice. In the laboratory, the skin samples underwent histotechnique process, Hematoxylin-Eosin (HE) staining, and Immunohistochemistry (IHC) with Oct-4 antibody staining. The data was analyzed using microscope, OptiLab™, Image Raster™, and SPSS. Oct-4 positive expression was counted and the data was examined with SPSS. The mean of Oct-4 expression in samples transported with dry ice was 2.30 and ice was 2.38. Our study resulted in no significant difference between dry ice and ice (P value is 0.901) in the Oct-4 expression. Thus, dry ice and ice have equal function as cold transport method for preputial skin and can be used as a bridge towards liquid nitrogen freezing.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"2007 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128587316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biologically Synthesized Gold Nanoparticles using Ocimum sanctum (Tulsi Leaf Extract) Induced Anti-Tumor Response in a T Cell Daltons Lymphoma 利用杜鹃花提取物生物合成金纳米颗粒诱导T细胞道尔顿淋巴瘤的抗肿瘤反应

Journal of Cell Science and Therapy Pub Date : 2017-12-19 DOI: 10.4172/2157-7013.1000278

P. K. Gautam, Sanjay Kumar, M. Tomar, R. Singh, A. Acharya, K. RitisShyanti, Anita, Sonal Swaroop, B. Ram

{"title":"Biologically Synthesized Gold Nanoparticles using Ocimum sanctum (Tulsi Leaf Extract) Induced Anti-Tumor Response in a T Cell Daltons Lymphoma","authors":"P. K. Gautam, Sanjay Kumar, M. Tomar, R. Singh, A. Acharya, K. RitisShyanti, Anita, Sonal Swaroop, B. Ram","doi":"10.4172/2157-7013.1000278","DOIUrl":"https://doi.org/10.4172/2157-7013.1000278","url":null,"abstract":"The characterizations of green synthesized gold nanoparticles (AuNPs) prepared using Ocimum sanctum leaf extract were done by UV-Spectrophotometer at 500-540 nm. The XRD data obtained were found similar to gold JCPDS File No- 04-0784. SEM and TEM analysis of AuNPs revealed spherical shape and size of 200 nm. Further FT-IR data indicated the various biomolecules present in Ocimum sanctum leaf extract provides stability to gold nanoparticles synthesis. The AuNPs were studied for their anti-cancer activity on Dalton’s lymphoma (DL) cells and the results obtained with IC50 value of < 50 ng/ml performed by MTT assay. Further, to confirm anti-tumor potential and the mode of action of the synthesized AuNPs, cell viability assay, nuclear morphology, DNA fragmentation assay, mitochondrial membrane potential (ΔΨm) analysis, and cell cycle analysis were done using DL cells. DL cells treated with the AuNPs showed reduced cell viability, altered nuclear morphology, typical apoptotic DNA ladder formation and apoptosis. From the above finding it can be concluded that the AuNPs have potential to decrease the proliferation of tumor cells and enhanced the production of ROS. Gold nanoparticles used in cancer detection and diagnosis/treatment are mainly in preclinical stages of cancer development.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129821772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Stem Cell Therapeutics of Acute Liver Diseases, Transplantation, andRegeneration 干细胞治疗急性肝脏疾病，移植，再生

Journal of Cell Science and Therapy Pub Date : 2017-08-03 DOI: 10.4172/2157-7013.1000275

R. Upadhyay

{"title":"Stem Cell Therapeutics of Acute Liver Diseases, Transplantation, andRegeneration","authors":"R. Upadhyay","doi":"10.4172/2157-7013.1000275","DOIUrl":"https://doi.org/10.4172/2157-7013.1000275","url":null,"abstract":"Present review article explains various causes of acute liver diseases and their therapeutics. This article describes major reasons of hepatic pathophysiological conditions and diseases including hepatitis, cholestasis, alcoholic and non-alcoholic steatohepatitis, jaundice, liver cirrhosis, carcinogenesis and many others. This article emphasizes use of proliferating hepatocytes, hepatic oval cells, adult human liver mesenchymal stem/progenitor cells, induced pluripotent stem cells (iPSCs) and hematopoietic stem cells in cell transplantation for restoration of liver structure and function. It also justified the therapeutic role of cell secreted growth factors and dietary factors required during natural healing and regeneration liver after surgery or liver transplantation. It sketches out regulatory roles of signaling pathways, expression of cell cycle regulators, growth factors, cytokines, and role of different mitogens in induction of liver stem/progenitor cells (LSPCs) after organ/stem cell transplantation. This article suggests a need for development of new advanced biomaterials, methods, technologies and stem cells for development of targeted therapies to combat and cure liver related diseases and disorders. This article also advice people for avoiding excessive use of alcohol, drugs, fats, salt, high energy diets, and iron as all are responsible of liver cirrhosis, damage and failure.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"71 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-08-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132457361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Modification Of Rat Testis Carbohydrate Determinants In PostnatalMorphogenesis As Detected By Lectin Probes 凝集素探针检测大鼠睾丸出生后形态发生中碳水化合物决定因素的改变

Journal of Cell Science and Therapy Pub Date : 2017-07-03 DOI: 10.4172/2157-7013.1000274

A. Shegedin, A. Yashchenko, A. Lutsyk

{"title":"Modification Of Rat Testis Carbohydrate Determinants In PostnatalMorphogenesis As Detected By Lectin Probes","authors":"A. Shegedin, A. Yashchenko, A. Lutsyk","doi":"10.4172/2157-7013.1000274","DOIUrl":"https://doi.org/10.4172/2157-7013.1000274","url":null,"abstract":"A panel of 15 peroxidase labeled lectins, supplemented with haematoxylin-eosin staining and PAS-reaction, were used to study modifications of carbohydrates in rat testis during postnatal morphogenesis, including prenatal day 20th, postnatal days 1st, 20th, 40th, in comparison to the adult rat testis. Tissue samples were fixed in Bouin’s fluid and embedded in paraffin. Lectin panel included Con A, PSA, GNA, NPA, PNA, VAA, PIFA, CNFA, CCRA, SBA, HPA, MPFA, WGA, SNA and LABA. It was detected that postnatal morphogenesis of rat testis is accompanied by active glycoconjugates rearrangement. In late prenatal and early postnatal development most intense lectin labeling was characteristic for fetal Leydig cells, number of which reduced significantly on the 1st postnatal day. Highest selectivity of these cells binding was documented with PSA, GNA and NPA. On postnatal day 20th was detected strong reactivity of developing spermatocytes with PSA, CNFA, HPA and WGA, and to a lesser extent– with other used lectins. This lectin binding increased on the postnatal day 40th, covering all subsets of multilayered spermatogenic epithelium in within the seminiferous tubules. Since postnatal day 40th onwards granule- and capstage pro-acrosomes, early and late acrosomes demonstrated strong reactivity with PNA, VAA, SBA, HPA, CNFA and SNA; most selective acrosomes labeling was detected with PNA and SBA. Majority of used lectins strongly reacted with glycoconjugate deposits, located in the adlumenal compartments of seminiferous tubules. Three original lectin preparartions (LABA, MPFA, PIFA) proved to be useful tools in andrology research.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121282048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

OSM-Stimulated Cardiomyocytes Release a C-Terminal Fragment ofFGF23 osm刺激心肌细胞释放c端片段ofFGF23

Journal of Cell Science and Therapy Pub Date : 2017-06-14 DOI: 10.4172/2157-7013.1000273

R. Maringanti, T. Kubin, A. Cetinkaya, M. Schönburg, Andres Beiras Fernandez, T. Braun, T. Walther, S. Kostin, M. Richter

{"title":"OSM-Stimulated Cardiomyocytes Release a C-Terminal Fragment ofFGF23","authors":"R. Maringanti, T. Kubin, A. Cetinkaya, M. Schönburg, Andres Beiras Fernandez, T. Braun, T. Walther, S. Kostin, M. Richter","doi":"10.4172/2157-7013.1000273","DOIUrl":"https://doi.org/10.4172/2157-7013.1000273","url":null,"abstract":"Background: Recent studies emphasize a correlation of increased FGF23 with the pathogenesis of heart diseases. Although it is widely assumed that the bone and not the heart is the major source of FGF23 we previously demonstrated that oncostatin M (OSM) activated cardiomyocytes strongly secrete FGF23. This phosphatonin can be released as intact molecule (iFGF23) as well as C-terminal (cFGF23) and N-terminal (nFGF23) fragments. Since cleavage does not only inactivate iFGF23 but might also exert antagonizing activity we wanted to determine which form is secreted by cardiomyocytes. \u0000Methods: Adult cultured cardiomyocytes were stimulated with OSM or albumin as control. Supernatant and cell lysate were analyzed by Western blot (WB) and specific ELISAs against cFGF23 as well as iFGF23. Expression of FGF23 in cardiomyocytes of 6 patients with coronary heart diseases (CHD) was analyzed by confocal microscopy because OSM signaling cascades are activated after myocardial infarction. \u0000Results: WB analysis identified cFGF23 as well as nFGF23 while iFGF23 was hardly detectable in the supernatant of OSM-stimulated cardiomyocytes. Analysis of the supernatant by ELISAs revealed that less than 3% of this secreted phosphatonin was intact. In patients with CHD the number of FGF23 positive cardiomyocytes increased from 0.2% in the remote zone to 4.4% in the border zone. \u0000Conclusions: The expression and release of FGF23 by cardiomyocytes indicate local as well as systemic functions. The determination of the ratio of iFGF23/cFGF23 will be essential to understand the functional role of this growth factor in patients with cardiac diseases.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126479847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

DNA Methylation in Cancer Tissues 癌症组织中的DNA甲基化

Journal of Cell Science and Therapy Pub Date : 2017-05-29 DOI: 10.4172/2157-7013.1000272

Daud Faran Asif, M. Naveed, U. Rashid

引用次数: 2

Dry Powder Inhaler Devices 干粉吸入器装置

Journal of Cell Science and Therapy Pub Date : 2017-05-26 DOI: 10.4172/2157-7013.1000271

Daud Faran Asif

引用次数: 3

Lithium Treatment in Clinical Medicine: History, Current Status andFuture Use 临床医学中的锂治疗:历史、现状和未来应用

Journal of Cell Science and Therapy Pub Date : 2017-05-18 DOI: 10.4172/2157-7013.1000270

Amy E Duvall, V. Gallicchio

{"title":"Lithium Treatment in Clinical Medicine: History, Current Status andFuture Use","authors":"Amy E Duvall, V. Gallicchio","doi":"10.4172/2157-7013.1000270","DOIUrl":"https://doi.org/10.4172/2157-7013.1000270","url":null,"abstract":"Lithium treatment was present in the first half of the twentieth century, but little to no attention was given to lithium in psychiatric literature at that time. This noticeable decline in the popularity of lithium could be attributed to the inquiries into lithia water and lithium tablets, which were frequently used in the late 1800s and early 1900s as lithium treatment delivery options. Lithium’s noticeable reappearance began in 1949 when John Cade used lithium to treat maniac patients, hypothesizing their sporadic excitement to be a result of a uric acid condition, which was also preventing normal discharge functioning. Lithium is a versatile drug that can be used in a variety of ways to reduce and prevent symptoms of various disorders. There are some areas of lithium research, however, that seem more promising in terms of yield ground-breaking treatment options for serious and currently incurable illnesses. Though ample research has been conducted to understand lithium’s abilities and effects, more is required to comprehensively assess how lithium works to counteract abnormalities contributing to disorders and diseases. Hematology, as well as many other fields of science, benefit from lithium use and need to continue to observe its capabilities.","PeriodicalId":150547,"journal":{"name":"Journal of Cell Science and Therapy","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2017-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133473917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8